FvH4_4g02220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011197
Physical Location & Seq
Forward (+)
2046827 .. 2052755
5929 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02220.t2

Sequence Viewer

Length: 669 bp
ATGGCAGTCTGCTCTTCACCACTCGCAGTTGTCTCTCCCAAACCATCCAAACTCACCTCTAAGCTATCGAAAACTAGAGTTACGGTTTCCTCACCTCACATTTACAGCCCCACCAAATCATCATTGCTCGGAATAAACTACTTGTCTAAGACAAGTTTCACGCGGTTGAACGCTGCTGGGTTCGCAGAGATCGAGCCTGATCTCAATGAAGACCCCACTGATCGTTGGGCGACCAACAGTGTCTCTGCTGAGGATTTTAAGTATGGAAAGTATGATGAGCACCACACTTACTATGAAGGAGAGGAGGAGAAAGGAACCTTTTGGGGATCAATAGCGGAAGACGTTGAAGGAGTAGGACCCCCTACAGGTTTTCAGGGTTTAATATCATGGCTTTTTCCACCTGCAATTGCTGCCGCAATGTATTTTCATGCACCGGGGGAGTACTTGTTCATTGGAGCCGGCCTATTTACAATTGTATTTTGTATTATTGAGATGGATAAGCCAGATCAACCCCACAACTTTGAGCCTCAGATATACAATATGGAGAGGGGATCTCGTGACAAGTTGATAGCTGATTACAATACCATGGACATCTGGGATTTTAATGAGAAATACGGGGACCTATGGGATTTCACCGTGAAGCCAAAGGATGATATAACAAAGAGATAA

Protein Analysis

223

Amino Acids

24.99

Weight (kDa)

4.89

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43750
fragaria_vesca FvH4_4g02220 FvH4_4g02220
malus_domestica MD11G1069500.v1.1
prunus_persica Prupe.1G026900_v2.0.a1
pyrus_communis pycom11g05790
rosa_chinensis RchiOBHm_Chr4g0389531
rosa_laevigata RLG00000009976
rosa_multiflora Rmu_sc0002498.1_g000005 Rmu_sc0016342.1_g000010
rosa_roxburghii Rroxscaffold_5G00335490
rosa_rugosa Rorug03G0323700 Rorug03G0323700
rosa_samantha Rh4AG028100 Rh4BG021200 Rh4CG030000 Rh4DG021800
rosa_wichuraiana Rw0G017320 Rw4G002040 Rw4G002080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 409
Acc36I ACCTGC 1 cut(s) 409
AccII CGCG 1 cut(s) 163
AciI CCGC 3 cut(s) 163, 335, 414
AclWI GGATC 2 cut(s) 334, 559
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 365
AgsI TTSAA 2 cut(s) 169, 347
AloI GAACNNNNNNTCC 2 cut(s) 431, 463
AluBI AGCT 2 cut(s) 64, 572
AluI AGCT 2 cut(s) 64, 572
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 2 cut(s) 37, 247
AlwI GGATC 2 cut(s) 334, 559
AoxI GGCC 1 cut(s) 460
ApeKI GCWGC 2 cut(s) 173, 410
AspS9I GGNCC 2 cut(s) 356, 619
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 4 cut(s) 9, 46, 84, 625
AvaII GGWCC 2 cut(s) 356, 619
BauI CACGAG 1 cut(s) 555
BbsI GAAGAC 2 cut(s) 216, 345
Bbv12I GWGCWC 1 cut(s) 282
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 160, 397
BccI CCATC 2 cut(s) 52, 487
BcnI CCSGG 1 cut(s) 435
BcoDI GTCTC 2 cut(s) 37, 247
BfaI CTAG 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 363
BfuAI ACCTGC 1 cut(s) 409
BisI GCNGC 3 cut(s) 174, 411, 414
BlsI GCNGC 3 cut(s) 175, 412, 415
BmcAI AGTACT 1 cut(s) 443
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 2 cut(s) 356, 619
BmgT120I GGNCC 2 cut(s) 356, 619
BmiI GGNNCC 4 cut(s) 316, 358, 457, 620
BmrFI CCNGG 1 cut(s) 435
BpiI GAAGAC 2 cut(s) 216, 345
BplI GAGNNNNNCTC 2 cut(s) 538, 570
Bpu10I CCTNAGC 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 435
BsaJI CCNNGG 2 cut(s) 434, 585
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 1 cut(s) 365
Bse118I RCCGGY 1 cut(s) 458
Bse3DI GCAATG 2 cut(s) 122, 423
BseDI CCNNGG 2 cut(s) 434, 585
BseGI GGATG 2 cut(s) 44, 655
BseLI CCNNNNNNNGG 1 cut(s) 365
BseMI GCAATG 2 cut(s) 122, 423
BseMII CTCAG 2 cut(s) 240, 542
BseRI GAGGAG 2 cut(s) 317, 320
BseXI GCAGC 2 cut(s) 160, 397
BseYI CCCAGC 1 cut(s) 176
Bsh1236I CGCG 1 cut(s) 163
BshFI GGCC 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 2 cut(s) 434, 459
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 1 cut(s) 365
BsmAI GTCTC 2 cut(s) 37, 247
BsmFI GGGAC 1 cut(s) 632
BsnI GGCC 1 cut(s) 462
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 6 cut(s) 189, 199, 220, 326, 505, 551
Bsp19I CCATGG 1 cut(s) 585
BspACI CCGC 3 cut(s) 163, 335, 414
BspANI GGCC 1 cut(s) 462
BspCNI CTCAG 2 cut(s) 241, 541
BspFNI CGCG 1 cut(s) 163
BspLI GGNNCC 4 cut(s) 316, 358, 457, 620
BspMI ACCTGC 1 cut(s) 409
BspPI GGATC 2 cut(s) 334, 559
BspQI GCTCTTC 1 cut(s) 19
BsrDI GCAATG 2 cut(s) 122, 423
BsrFI RCCGGY 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 2 cut(s) 434, 585
BssMI GATC 6 cut(s) 189, 199, 220, 326, 505, 551
BssSI CACGAG 1 cut(s) 555
BssT1I CCWWGG 1 cut(s) 585
Bst2BI CACGAG 1 cut(s) 555
Bst4CI ACNGT 3 cut(s) 85, 239, 637
Bst6I CTCTTC 1 cut(s) 19
BstAPI GCANNNNNTGC 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 460
BstDEI CTNAG 4 cut(s) 60, 147, 249, 528
BstDSI CCRYGG 1 cut(s) 585
BstF5I GGATG 2 cut(s) 44, 655
BstFNI CGCG 1 cut(s) 163
BstKTI GATC 6 cut(s) 192, 202, 223, 329, 508, 554
BstMAI GTCTC 2 cut(s) 37, 247
BstMBI GATC 6 cut(s) 189, 199, 220, 326, 505, 551
BstMWI GCNNNNNNNGC 2 cut(s) 182, 410
BstSCI CCNGG 1 cut(s) 433
BstSFI CTRYAG 1 cut(s) 363
BstUI CGCG 1 cut(s) 163
BstV1I GCAGC 2 cut(s) 160, 397
BstV2I GAAGAC 2 cut(s) 216, 345
BstX2I RGATCY 1 cut(s) 551
BstYI RGATCY 1 cut(s) 551
BsuRI GGCC 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 585
BtsCI GGATG 2 cut(s) 44, 655
BtsIMutI CAGTG 2 cut(s) 216, 244
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 1 cut(s) 460
Cfr10I RCCGGY 1 cut(s) 458
Cfr13I GGNCC 2 cut(s) 356, 619
Csp6I GTAC 1 cut(s) 442
CviAII CATG 3 cut(s) 387, 428, 586
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 4 cut(s) 60, 147, 249, 528
DpnI GATC 6 cut(s) 191, 201, 222, 328, 507, 553
DpnII GATC 6 cut(s) 189, 199, 220, 326, 505, 551
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 585
Eco47I GGWCC 2 cut(s) 356, 619
EcoO109I RGGNCCY 2 cut(s) 356, 619
EcoT14I CCWWGG 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 585
FaeI CATG 3 cut(s) 390, 431, 589
FaqI GGGAC 1 cut(s) 632
FatI CATG 3 cut(s) 386, 427, 585
Fnu4HI GCNGC 3 cut(s) 174, 411, 414
FokI GGATG 2 cut(s) 31, 662
Fsp4HI GCNGC 3 cut(s) 174, 411, 414
FspBI CTAG 1 cut(s) 75
GluI GCNGC 3 cut(s) 174, 411, 414
GsaI CCCAGC 1 cut(s) 180
HaeIII GGCC 1 cut(s) 462
HapII CCGG 2 cut(s) 434, 459
Hin1II CATG 3 cut(s) 390, 431, 589
HpaII CCGG 2 cut(s) 434, 459
HphI GGTGA 4 cut(s) 9, 46, 84, 625
Hpy188I TCNGA 2 cut(s) 131, 531
Hpy188III TCNNGA 1 cut(s) 557
HpyAV CCTTC 2 cut(s) 290, 341
HpyCH4III ACNGT 3 cut(s) 85, 239, 637
HpyCH4IV ACGT 1 cut(s) 342
HpyCH4V TGCA 2 cut(s) 404, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 410
HpyF3I CTNAG 4 cut(s) 60, 147, 249, 528
HpySE526I ACGT 1 cut(s) 342
Hsp92II CATG 3 cut(s) 390, 431, 589
KroI GCCGGC 1 cut(s) 458
KroNI GCCGGC 1 cut(s) 460
Kzo9I GATC 6 cut(s) 189, 199, 220, 326, 505, 551
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 1 cut(s) 455
LpnPI CCDG 9 cut(s) 162, 210, 351, 359, 414, 447, 472, 516, 580
Lsp1109I GCAGC 2 cut(s) 160, 397
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 342
MaeIII GTNAC 2 cut(s) 79, 557
MalI GATC 6 cut(s) 191, 201, 222, 328, 507, 553
MboI GATC 6 cut(s) 189, 199, 220, 326, 505, 551
MboII GAAGA 3 cut(s) 6, 221, 350
MfeI CAATTG 2 cut(s) 405, 471
MflI RGATCY 1 cut(s) 551
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 2 cut(s) 405, 471
MnlI CCTC 8 cut(s) 67, 100, 105, 244, 295, 298, 537, 540
MroNI GCCGGC 1 cut(s) 458
MseI TTAA 3 cut(s) 258, 380, 603
MspI CCGG 2 cut(s) 434, 459
MspR9I CCNGG 1 cut(s) 435
MunI CAATTG 2 cut(s) 405, 471
MvnI CGCG 1 cut(s) 163
MwoI GCNNNNNNNGC 2 cut(s) 182, 410
NaeI GCCGGC 1 cut(s) 460
NciI CCSGG 1 cut(s) 435
NcoI CCATGG 1 cut(s) 585
NdeII GATC 6 cut(s) 189, 199, 220, 326, 505, 551
NgoMIV GCCGGC 1 cut(s) 458
NlaIII CATG 3 cut(s) 390, 431, 589
NlaIV GGNNCC 4 cut(s) 316, 358, 457, 620
NmuCI GTSAC 1 cut(s) 557
PaqCI CACCTGC 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 19
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PdiI GCCGGC 1 cut(s) 460
PkrI GCNGC 3 cut(s) 175, 412, 415
PpuMI RGGWCCY 2 cut(s) 356, 619
Psp5II RGGWCCY 2 cut(s) 356, 619
PspFI CCCAGC 1 cut(s) 176
PspN4I GGNNCC 4 cut(s) 316, 358, 457, 620
PspPI GGNCC 2 cut(s) 356, 619
PspPPI RGGWCCY 2 cut(s) 356, 619
PsuI RGATCY 1 cut(s) 551
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 3 cut(s) 258, 380, 603
SatI GCNGC 3 cut(s) 174, 411, 414
Sau3AI GATC 6 cut(s) 189, 199, 220, 326, 505, 551
Sau96I GGNCC 2 cut(s) 356, 619
ScaI AGTACT 1 cut(s) 443
ScrFI CCNGG 1 cut(s) 435
SduI GDGCHC 1 cut(s) 282
SetI ASST 9 cut(s) 59, 66, 97, 320, 345, 370, 403, 574, 624
SfcI CTRYAG 1 cut(s) 363
SinI GGWCC 2 cut(s) 356, 619
Sse9I AATT 2 cut(s) 405, 471
SsiI CCGC 3 cut(s) 163, 335, 414
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 3 cut(s) 85, 239, 637
TaiI ACGT 1 cut(s) 345
TaqI TCGA 2 cut(s) 68, 192
TasI AATT 2 cut(s) 405, 471
TatI WGTACW 1 cut(s) 441
TauI GCSGC 1 cut(s) 416
Tru1I TTAA 3 cut(s) 258, 380, 603
Tru9I TTAA 3 cut(s) 258, 380, 603
TscAI CASTG 2 cut(s) 223, 244
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 2 cut(s) 173, 410
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 4 cut(s) 222, 309, 416, 439
TspRI CASTG 2 cut(s) 223, 244
VpaK11BI GGWCC 2 cut(s) 356, 619
XspI CTAG 1 cut(s) 75
ZrmI AGTACT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.