Rw4G002040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011197
Physical Location & Seq
Forward (+)
3759209 .. 3763122
3914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G002040.1

Sequence Viewer

Length: 660 bp
ATGGCAGTCTGCTCCTCTCCACTCGCAGTTGTCTCTCCCAACCCAGCACCACTCCCCAGACTCAGCTCTAAGCTATCGCAAACTAGGCTTAACGTTTCCTCGGGTCACATTCACAGCCCCTCCAAATTCCTAAACTTGCCTAAGAGAAGTTTCACACGGTTGAACGCTGCTGGGTTCGCAGAGATCGAGCCTGACCTCAATGAAGACCCCACTAACCGTTGGGAAACTAACAGTATCTCCGCGGAGGATTTTAAGTATGGAGACTATGATGACCACCATACTTACCATGAAGGAGAGGAGACAGGAACCTTCTGGGGATCAATAGCAGAAGATGTTGAAGCAGTAGGACCCCCTACAGGTTTTCAGGGTTTAATATCATGGCTTTTCCCCCCTGCAGTTGCTGCCGCAATGTATTTTCATGCCCCGGGGGAGTACTTGTTCATTGGAGCGGGTCTATTTACGATTGTATTTTGTATTATTGAGATGGATAAGCCAGATCAACCCCACAACTTTGAGCCTCAGATATACAATATGGAGAGGGGATCTCGTGACAAGTTGATAGCTGATTACAATACCATGGATATCTGGGATTTCAATGAGAAATATGGGGACCTATGGGATTTCACTGTGAAGGCGAAGGATGATATAACAAAGAGATAA

Protein Analysis

219

Amino Acids

24.65

Weight (kDa)

4.79

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011197)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43750
fragaria_vesca FvH4_4g02220 FvH4_4g02220
malus_domestica MD11G1069500.v1.1
prunus_persica Prupe.1G026900_v2.0.a1
pyrus_communis pycom11g05790
rosa_chinensis RchiOBHm_Chr4g0389531
rosa_laevigata RLG00000009976
rosa_multiflora Rmu_sc0002498.1_g000005 Rmu_sc0016342.1_g000010
rosa_roxburghii Rroxscaffold_5G00335490
rosa_rugosa Rorug03G0323700 Rorug03G0323700
rosa_samantha Rh4AG028100 Rh4BG021200 Rh4CG030000 Rh4DG021800
rosa_wichuraiana Rw0G017320 Rw4G002040 Rw4G002080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 449
AccII CGCG 1 cut(s) 242
AciI CCGC 4 cut(s) 240, 242, 405, 449
AclI AACGTT 1 cut(s) 93
AclWI GGATC 2 cut(s) 325, 550
AcsI RAATTY 1 cut(s) 125
AfaI GTAC 1 cut(s) 434
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 3 cut(s) 163, 338, 595
AloI GAACNNNNNNTCC 2 cut(s) 422, 454
AluBI AGCT 3 cut(s) 66, 73, 563
AluI AGCT 3 cut(s) 66, 73, 563
Alw26I GTCTC 3 cut(s) 37, 255, 293
AlwI GGATC 2 cut(s) 325, 550
AlwNI CAGNNNCTG 1 cut(s) 401
Ama87I CYCGRG 2 cut(s) 100, 424
ApeKI GCWGC 2 cut(s) 167, 401
ApoI RAATTY 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 347, 610
AsuC2I CCSGG 2 cut(s) 425, 426
AvaI CYCGRG 2 cut(s) 100, 424
AvaII GGWCC 2 cut(s) 347, 610
BauI CACGAG 1 cut(s) 546
BbsI GAAGAC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 154, 388
BccI CCATC 1 cut(s) 478
BcnI CCSGG 2 cut(s) 425, 426
BcoDI GTCTC 3 cut(s) 37, 255, 293
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 2 cut(s) 354, 393
BisI GCNGC 3 cut(s) 168, 402, 405
BlsI GCNGC 3 cut(s) 169, 403, 406
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 2 cut(s) 425, 426
Bme18I GGWCC 2 cut(s) 347, 610
BmeT110I CYCGRG 2 cut(s) 100, 424
BmgT120I GGNCC 2 cut(s) 347, 610
BmiI GGNNCC 3 cut(s) 307, 349, 611
BmrFI CCNGG 2 cut(s) 425, 426
BpiI GAAGAC 1 cut(s) 210
BplI GAGNNNNNCTC 2 cut(s) 529, 561
BpuMI CCSGG 2 cut(s) 425, 426
BsaJI CCNNGG 6 cut(s) 99, 240, 423, 424, 425, 576
BsaXI ACNNNNNCTCC 6 cut(s) 104, 134, 221, 251, 422, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 414
BseDI CCNNGG 6 cut(s) 99, 240, 423, 424, 425, 576
BseGI GGATG 1 cut(s) 646
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 2 cut(s) 76, 533
BseRI GAGGAG 2 cut(s) 4, 311
BseXI GCAGC 2 cut(s) 154, 388
BseYI CCCAGC 2 cut(s) 43, 170
Bsh1236I CGCG 1 cut(s) 242
BsiHKCI CYCGRG 2 cut(s) 100, 424
BsiSI CCGG 1 cut(s) 425
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 3 cut(s) 37, 255, 293
BsmFI GGGAC 1 cut(s) 623
BsoBI CYCGRG 2 cut(s) 100, 424
Bsp143I GATC 4 cut(s) 183, 317, 496, 542
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 4 cut(s) 240, 242, 405, 449
BspCNI CTCAG 2 cut(s) 75, 532
BspFNI CGCG 1 cut(s) 242
BspLI GGNNCC 3 cut(s) 307, 349, 611
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 2 cut(s) 325, 550
BsrBI CCGCTC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 414
BssECI CCNNGG 6 cut(s) 99, 240, 423, 424, 425, 576
BssMI GATC 4 cut(s) 183, 317, 496, 542
BssSI CACGAG 1 cut(s) 546
BssT1I CCWWGG 1 cut(s) 576
Bst2BI CACGAG 1 cut(s) 546
Bst4CI ACNGT 4 cut(s) 159, 218, 233, 628
BstAPI GCANNNNNTGC 1 cut(s) 401
BstDEI CTNAG 4 cut(s) 62, 69, 141, 519
BstDSI CCRYGG 2 cut(s) 240, 576
BstF5I GGATG 1 cut(s) 646
BstFNI CGCG 1 cut(s) 242
BstKTI GATC 4 cut(s) 186, 320, 499, 545
BstMAI GTCTC 3 cut(s) 37, 255, 293
BstMBI GATC 4 cut(s) 183, 317, 496, 542
BstMWI GCNNNNNNNGC 3 cut(s) 85, 176, 401
BstSCI CCNGG 2 cut(s) 423, 424
BstSFI CTRYAG 2 cut(s) 354, 393
BstUI CGCG 1 cut(s) 242
BstV1I GCAGC 2 cut(s) 154, 388
BstV2I GAAGAC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 542
BstYI RGATCY 1 cut(s) 542
BtgI CCRYGG 2 cut(s) 240, 576
BtsCI GGATG 1 cut(s) 646
BtsIMutI CAGTG 1 cut(s) 624
CaiI CAGNNNCTG 1 cut(s) 401
Cfr13I GGNCC 2 cut(s) 347, 610
Cfr42I CCGCGG 1 cut(s) 243
Cfr9I CCCGGG 1 cut(s) 424
Csp6I GTAC 1 cut(s) 433
CviAII CATG 4 cut(s) 287, 378, 419, 577
CviJI RGCY 9 cut(s) 66, 73, 88, 117, 190, 382, 493, 517, 563
CviKI_1 RGCY 9 cut(s) 66, 73, 88, 117, 190, 382, 493, 517, 563
CviQI GTAC 1 cut(s) 433
DdeI CTNAG 4 cut(s) 62, 69, 141, 519
DpnI GATC 4 cut(s) 185, 319, 498, 544
DpnII GATC 4 cut(s) 183, 317, 496, 542
Eco130I CCWWGG 1 cut(s) 576
Eco32I GATATC 1 cut(s) 583
Eco47I GGWCC 2 cut(s) 347, 610
Eco88I CYCGRG 2 cut(s) 100, 424
EcoO109I RGGNCCY 2 cut(s) 347, 610
EcoRV GATATC 1 cut(s) 583
EcoT14I CCWWGG 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 576
FaeI CATG 4 cut(s) 290, 381, 422, 580
FaqI GGGAC 1 cut(s) 623
FatI CATG 4 cut(s) 286, 377, 418, 576
FauI CCCGC 1 cut(s) 442
Fnu4HI GCNGC 3 cut(s) 168, 402, 405
FokI GGATG 1 cut(s) 653
Fsp4HI GCNGC 3 cut(s) 168, 402, 405
FspBI CTAG 1 cut(s) 84
GluI GCNGC 3 cut(s) 168, 402, 405
GsaI CCCAGC 2 cut(s) 47, 174
HapII CCGG 1 cut(s) 425
Hin1II CATG 4 cut(s) 290, 381, 422, 580
HinfI GANTC 1 cut(s) 60
HpaII CCGG 1 cut(s) 425
Hpy188I TCNGA 1 cut(s) 522
Hpy188III TCNNGA 1 cut(s) 548
HpyAV CCTTC 4 cut(s) 284, 319, 625, 631
HpyCH4III ACNGT 4 cut(s) 159, 218, 233, 628
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 1 cut(s) 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 176, 401
HpyF3I CTNAG 4 cut(s) 62, 69, 141, 519
HpySE526I ACGT 1 cut(s) 93
Hsp92II CATG 4 cut(s) 290, 381, 422, 580
KspI CCGCGG 1 cut(s) 243
Kzo9I GATC 4 cut(s) 183, 317, 496, 542
LmnI GCTCC 2 cut(s) 17, 446
Lsp1109I GCAGC 2 cut(s) 154, 388
MaeI CTAG 1 cut(s) 84
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 2 cut(s) 104, 548
MalI GATC 4 cut(s) 185, 319, 498, 544
MbiI CCGCTC 1 cut(s) 449
MboI GATC 4 cut(s) 183, 317, 496, 542
MboII GAAGA 2 cut(s) 215, 341
MflI RGATCY 1 cut(s) 542
MluCI AATT 1 cut(s) 125
MlyI GAGTC 1 cut(s) 54
MnlI CCTC 8 cut(s) 25, 109, 130, 206, 238, 289, 528, 531
MseI TTAA 3 cut(s) 90, 252, 371
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 2 cut(s) 425, 426
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 3 cut(s) 85, 176, 401
NciI CCSGG 2 cut(s) 425, 426
NcoI CCATGG 1 cut(s) 576
NdeII GATC 4 cut(s) 183, 317, 496, 542
NlaIII CATG 4 cut(s) 290, 381, 422, 580
NlaIV GGNNCC 3 cut(s) 307, 349, 611
NmuCI GTSAC 2 cut(s) 104, 548
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PkrI GCNGC 3 cut(s) 169, 403, 406
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PpuMI RGGWCCY 2 cut(s) 347, 610
Psp1406I AACGTT 1 cut(s) 93
Psp5II RGGWCCY 2 cut(s) 347, 610
PspFI CCCAGC 2 cut(s) 43, 170
PspN4I GGNNCC 3 cut(s) 307, 349, 611
PspPI GGNCC 2 cut(s) 347, 610
PspPPI RGGWCCY 2 cut(s) 347, 610
PstI CTGCAG 1 cut(s) 397
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 1 cut(s) 542
RsaI GTAC 1 cut(s) 434
RsaNI GTAC 1 cut(s) 433
SacII CCGCGG 1 cut(s) 243
SaqAI TTAA 3 cut(s) 90, 252, 371
SatI GCNGC 3 cut(s) 168, 402, 405
Sau3AI GATC 4 cut(s) 183, 317, 496, 542
Sau96I GGNCC 2 cut(s) 347, 610
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 2 cut(s) 425, 426
SetI ASST 8 cut(s) 68, 75, 96, 198, 311, 361, 565, 615
SfcI CTRYAG 2 cut(s) 354, 393
Sfr303I CCGCGG 1 cut(s) 243
SgrBI CCGCGG 1 cut(s) 243
SinI GGWCC 2 cut(s) 347, 610
SmaI CCCGGG 1 cut(s) 426
Sse9I AATT 1 cut(s) 125
SsiI CCGC 4 cut(s) 240, 242, 405, 449
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 423, 424
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 4 cut(s) 159, 218, 233, 628
TaiI ACGT 1 cut(s) 96
TaqI TCGA 1 cut(s) 186
TasI AATT 1 cut(s) 125
TatI WGTACW 1 cut(s) 432
TauI GCSGC 1 cut(s) 407
Tru1I TTAA 3 cut(s) 90, 252, 371
Tru9I TTAA 3 cut(s) 90, 252, 371
TscAI CASTG 1 cut(s) 631
TseFI GTSAC 2 cut(s) 104, 548
TseI GCWGC 2 cut(s) 167, 401
Tsp45I GTSAC 2 cut(s) 104, 548
TspDTI ATGAA 4 cut(s) 216, 303, 407, 430
TspMI CCCGGG 1 cut(s) 424
TspRI CASTG 1 cut(s) 631
VpaK11BI GGWCC 2 cut(s) 347, 610
XapI RAATTY 1 cut(s) 125
XmaI CCCGGG 1 cut(s) 424
XspI CTAG 1 cut(s) 84
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.