FvH4_4g03370

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012397
Physical Location & Seq
Forward (+)
3053357 .. 3055924
2568 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g03370.t1

Sequence Viewer

Length: 570 bp
ATGAAGGATTTTCCTGGGACTCCTGGGACTTTGACTGCGCTTCTTCTCAGGATTTCACAATGCGTCTTAGCTGCTGCCTCTATCGCTTCCATGGCCACCAGTTCCAGCTTCTTCAATTTCACAGCTTTCTGCTACCTGATTGCTTCAATGGGTCTGCAAGTGATTTGGAGTTTTGTTCTGGCATTGTTAGATGCATACTCCTTGATGAAAAAGAAGGCCCTCCACAACCCTGTATTGGTCAGCCTCTTTGTAGTTGGAGACTGGGTTACTGCAACACTGTCTCTAGCAGCTGCTTCTGCTTCAGCTGGAATAACTGTCTTGTACTTCAGTGACTTGAGCCACTGCAGTTTTGGAGAGGAATGCCAGAAATACCAGATGGCAGTTGCTTTTGCTTACCTGAGTTGGGTAACAATTGCTATTTCTTCTCTAATCATGCTCTGGCTATTGGCAGCAGATCAAGCCTATGTTTCAAGGAGAACGCAGATCAAGCCTTTGATTCTTTCTCCAATTCAAGCTTCATCGCCAATATCAACCATCAACCCAACACCAAAATCACTAGTTCAAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

190

Amino Acids

20.34

Weight (kDa)

8.72

Isoelectric Point (pI)

29.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 7 - 136 1.1e-26 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23200
fragaria_vesca FvH4_4g03370 FvH4_4g03370
malus_domestica MD13G1212200.v1.1
prunus_persica Prupe.1G037100_v2.0.a1 Prupe.1G037100_v2.0.a1
pyrus_communis pycom13g18400
rosa_chinensis RchiOBHm_Chr4g0392361
rosa_laevigata RLG00000009817
rosa_multiflora Rmu_sc0001800.1_g000025
rosa_roxburghii Rroxscaffold_5G00337540
rosa_rugosa Rorug03G0338500 Rorug03G0338600 Rorug03G0338600
rosa_samantha Rh4AG042100 Rh4BG037000 Rh4CG044900 Rh4DG039100
rosa_wichuraiana Rw4G003280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 93
AcuI CTGAAG 2 cut(s) 285, 310
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 2 cut(s) 235, 403
AgsI TTSAA 5 cut(s) 115, 147, 471, 512, 563
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 2 cut(s) 13, 22
AjuI GAANNNNNNNTTGG 2 cut(s) 499, 531
AluBI AGCT 6 cut(s) 71, 108, 125, 290, 305, 515
AluI AGCT 6 cut(s) 71, 108, 125, 290, 305, 515
Alw26I GTCTC 2 cut(s) 252, 285
AoxI GGCC 2 cut(s) 93, 216
ApeKI GCWGC 5 cut(s) 71, 74, 287, 290, 449
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 217
BalI TGGCCA 1 cut(s) 95
BbvI GCAGC 5 cut(s) 58, 61, 277, 299, 461
BccI CCATC 2 cut(s) 370, 542
BciT130I CCWGG 2 cut(s) 15, 24
BcoDI GTCTC 2 cut(s) 252, 285
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 2 cut(s) 284, 557
BfmI CTRYAG 1 cut(s) 343
BisI GCNGC 5 cut(s) 72, 75, 288, 291, 450
BlsI GCNGC 5 cut(s) 73, 76, 289, 292, 451
Bme1390I CCNGG 2 cut(s) 15, 24
BmgT120I GGNCC 1 cut(s) 217
BmrFI CCNGG 2 cut(s) 15, 24
BmrI ACTGGG 1 cut(s) 271
BmsI GCATC 1 cut(s) 181
BmuI ACTGGG 1 cut(s) 271
BpuEI CTTGAG 1 cut(s) 355
BsaJI CCNNGG 3 cut(s) 14, 23, 90
BsaXI ACNNNNNCTCC 2 cut(s) 249, 279
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 403
Bse1I ACTGG 2 cut(s) 99, 266
BseBI CCWGG 2 cut(s) 15, 24
BseDI CCNNGG 3 cut(s) 14, 23, 90
BseLI CCNNNNNNNGG 2 cut(s) 235, 403
BseMII CTCAG 2 cut(s) 61, 389
BseNI ACTGG 2 cut(s) 99, 266
BseXI GCAGC 5 cut(s) 58, 61, 277, 299, 461
BshFI GGCC 2 cut(s) 95, 218
BslFI GGGAC 2 cut(s) 31, 40
BslI CCNNNNNNNGG 2 cut(s) 235, 403
BsmAI GTCTC 2 cut(s) 252, 285
BsmFI GGGAC 2 cut(s) 31, 40
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 2 cut(s) 95, 218
Bsp143I GATC 2 cut(s) 454, 483
Bsp19I CCATGG 1 cut(s) 90
BspANI GGCC 2 cut(s) 95, 218
BspCNI CTCAG 2 cut(s) 60, 390
BspMAI CTGCAG 1 cut(s) 347
BsrI ACTGG 2 cut(s) 99, 266
BssECI CCNNGG 3 cut(s) 14, 23, 90
BssMI GATC 2 cut(s) 454, 483
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 2 cut(s) 15, 24
Bst4CI ACNGT 2 cut(s) 279, 316
BstDEI CTNAG 3 cut(s) 47, 67, 398
BstDSI CCRYGG 1 cut(s) 90
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 2 cut(s) 457, 486
BstMAI GTCTC 2 cut(s) 252, 285
BstMBI GATC 2 cut(s) 454, 483
BstMWI GCNNNNNNNGC 5 cut(s) 83, 92, 296, 458, 487
BstNI CCWGG 2 cut(s) 15, 24
BstSCI CCNGG 2 cut(s) 13, 22
BstSFI CTRYAG 1 cut(s) 343
BstV1I GCAGC 5 cut(s) 58, 61, 277, 299, 461
BsuRI GGCC 2 cut(s) 95, 218
BtgI CCRYGG 1 cut(s) 90
BtgZI GCGATG 1 cut(s) 504
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 3 cut(s) 275, 334, 340
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 217
CseI GACGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 322
CviAII CATG 2 cut(s) 91, 433
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 3 cut(s) 47, 67, 398
DpnI GATC 2 cut(s) 456, 485
DpnII GATC 2 cut(s) 454, 483
EaeI YGGCCR 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 90
Eco57I CTGAAG 2 cut(s) 285, 310
EcoO109I RGGNCCY 1 cut(s) 217
EcoRII CCWGG 2 cut(s) 13, 22
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 2 cut(s) 94, 436
FaiI YATR 5 cut(s) 92, 196, 434, 465, 568
FaqI GGGAC 2 cut(s) 31, 40
FatI CATG 2 cut(s) 90, 432
Fnu4HI GCNGC 5 cut(s) 72, 75, 288, 291, 450
Fsp4HI GCNGC 5 cut(s) 72, 75, 288, 291, 450
FspBI CTAG 2 cut(s) 284, 557
GlaI GCGC 1 cut(s) 39
GluI GCNGC 5 cut(s) 72, 75, 288, 291, 450
HaeIII GGCC 2 cut(s) 95, 218
HgaI GACGC 1 cut(s) 52
HhaI GCGC 1 cut(s) 40
Hin1II CATG 2 cut(s) 94, 436
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 513
HinfI GANTC 2 cut(s) 19, 496
Hpy188III TCNNGA 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 2 cut(s) 279, 316
HpyCH4V TGCA 4 cut(s) 157, 194, 272, 345
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 92, 296, 458, 487
HpyF3I CTNAG 3 cut(s) 47, 67, 398
Hsp92II CATG 2 cut(s) 94, 436
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 2 cut(s) 454, 483
Lsp1109I GCAGC 5 cut(s) 58, 61, 277, 299, 461
LweI GCATC 1 cut(s) 181
MaeI CTAG 2 cut(s) 284, 557
MaeIII GTNAC 3 cut(s) 265, 329, 406
MalI GATC 2 cut(s) 456, 485
MboI GATC 2 cut(s) 454, 483
MboII GAAGA 3 cut(s) 35, 103, 414
MfeI CAATTG 1 cut(s) 411
MlsI TGGCCA 1 cut(s) 95
MluCI AATT 3 cut(s) 115, 411, 507
MluNI TGGCCA 1 cut(s) 95
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 235
MnlI CCTC 4 cut(s) 88, 230, 254, 349
Mox20I TGGCCA 1 cut(s) 95
Mph1103I ATGCAT 1 cut(s) 196
MscI TGGCCA 1 cut(s) 95
Msp20I TGGCCA 1 cut(s) 95
MspA1I CMGCKG 2 cut(s) 290, 305
MspR9I CCNGG 2 cut(s) 15, 24
MunI CAATTG 1 cut(s) 411
Mva1269I GAATGC 1 cut(s) 365
MvaI CCWGG 2 cut(s) 15, 24
MwoI GCNNNNNNNGC 5 cut(s) 83, 92, 296, 458, 487
NcoI CCATGG 1 cut(s) 90
NdeII GATC 2 cut(s) 454, 483
NlaIII CATG 2 cut(s) 94, 436
NmuCI GTSAC 1 cut(s) 329
NsiI ATGCAT 1 cut(s) 196
PctI GAATGC 1 cut(s) 365
PfeI GAWTC 1 cut(s) 496
PkrI GCNGC 5 cut(s) 73, 76, 289, 292, 451
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp6I CCWGG 2 cut(s) 13, 22
PspGI CCWGG 2 cut(s) 13, 22
PspPI GGNCC 1 cut(s) 217
PstI CTGCAG 1 cut(s) 347
PvuII CAGCTG 2 cut(s) 290, 305
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SatI GCNGC 5 cut(s) 72, 75, 288, 291, 450
Sau3AI GATC 2 cut(s) 454, 483
Sau96I GGNCC 1 cut(s) 217
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 15, 24
SetI ASST 8 cut(s) 73, 110, 127, 138, 292, 307, 399, 517
SfaNI GCATC 1 cut(s) 181
SfcI CTRYAG 1 cut(s) 343
SmlI CTYRAG 1 cut(s) 334
SmoI CTYRAG 1 cut(s) 334
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 3 cut(s) 115, 411, 507
SspMI CTAG 2 cut(s) 284, 557
StyD4I CCNGG 2 cut(s) 13, 22
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 279, 316
TasI AATT 3 cut(s) 115, 411, 507
TatI WGTACW 1 cut(s) 321
TfiI GAWTC 1 cut(s) 496
TscAI CASTG 3 cut(s) 282, 334, 347
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 5 cut(s) 71, 74, 287, 290, 449
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 3 cut(s) 17, 221, 507
TspRI CASTG 3 cut(s) 282, 334, 347
XcmI CCANNNNNNNNNTGG 1 cut(s) 347
XspI CTAG 2 cut(s) 284, 557
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.