FvH4_4g04570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006079
Physical Location & Seq
Forward (+)
3909387 .. 3911762
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04570.t1

Sequence Viewer

Length: 1275 bp
ATGTGGCGCAGAAGACCTGGACTCCGCGCCGTTCGGAACCTCCGGCAGCTCTCAACAGCAATACGCCGCCGTATCACTGACGAGGGCAACTGGTCCTACGCCTCCGAATGGTGGGGCACCGAATCCGACGGCCGCGTCGTCCTCCGCTCCACATCCGAAAAGGGAAACGGCGTCGTCTCCGTCGCCGCTTTCCCCGCCTCTAAACCCAGCAAGCTTCACTGGGCAGAGACCGAGAGATGGCTAGAGAAGAGGTACAAAGAGATAGACGAATCCCATGATTACAATGAGAAGTTCAGAGTTGTTGGATATGAATGGCGCTGTCTTCGTTTTAACGACGACACTCGCCAGAGCACTGTCAAGGTCTTGGCCGCTTTCCGGCAATCGGAGCCGGCGTGTTTTGCTCTTATGCAGCAACCTCATTGCCTCGCTGTTCCATATCTTAAGAGTATGTTATCAGTTGGGTTGGCTAGTATAGCTTGTTGTGATTACAATCTGAAGAAGGTGGCGAATGGGAAGGATACGATGCGGGTTTTATGCATCGGCCATGGTGGAGGGAGCTTGCCATTGTTTTTGGCTAGTAAACTTCAAGGTGCTATGGTTGATATAGTTGAAATTGATCCTGTTGTGATCTCAGCCTCGGTTCAAGCTATGGGGTTTCCTGCTTACTCGGTTATGAATCCGTCAGGCGAGCGTGCTGTTTCAAAACCTGATATTATGGATGAGGTAATGTGGAAAGGCATCCATGAGAGGCTGTTTCTCTATGAATCAGATGCTGAGGATTTCATTCTCAAAACTAACAATGTGTATGATATGATCTTCATTGATGCTTATGACGGCGAAGACATATTCCCTCGCAAGTTGTGGGACACAGATTCACCGTTTATGAAAGCTTTAAGCAGTCGGCTTCACCCCGATCATGGAACGGTTGTGGTGAACCTTCATTCAGATTGTGAGGTTTTGAATCATGATGGCTCTGTTCCTTCCATTCTTGAGCAAGTTTTGCCGATGGGAAAGTATGTCTCCCAGGTTTGCCAAGCATACAAAAATGTGGTAGTGGGCAGTCGAGATTCTGGTTTGGGGTTTACTGTTTCGGTTCCTTGGATCTGCAATATGTCTATGGTTGTTTGCAGAGGTTTTGGGATTAACAGTGGGTACACAAACAGGGATGTGATGTTGCATACCCTCATGTCCAAATCATTTGAAGTTGAAAATATTCTCAACTTACCATTCTCATGTTTGCAATATGTAAAGAGAGGTTTTAGACTTTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

425

Amino Acids

47.93

Weight (kDa)

7.12

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 116
AccBSI CCGCTC 1 cut(s) 147
AccII CGCG 2 cut(s) 27, 135
AciI CCGC 8 cut(s) 25, 67, 133, 145, 186, 195, 369, 526
AclWI GGATC 2 cut(s) 611, 1109
AcoI YGGCCR 3 cut(s) 130, 366, 541
AcuI CTGAAG 1 cut(s) 515
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 2 cut(s) 254, 1154
AfiI CCNNNNNNNGG 6 cut(s) 108, 111, 237, 375, 382, 917
AflII CTTAAG 1 cut(s) 440
AgsI TTSAA 7 cut(s) 587, 611, 644, 702, 961, 1202, 1208
AjnI CCWGG 2 cut(s) 16, 1023
AluBI AGCT 6 cut(s) 49, 214, 476, 558, 647, 890
AluI AGCT 6 cut(s) 49, 214, 476, 558, 647, 890
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 3 cut(s) 181, 221, 1024
AlwI GGATC 2 cut(s) 611, 1109
AlwNI CAGNNNCTG 1 cut(s) 773
AoxI GGCC 3 cut(s) 130, 366, 541
ApeKI GCWGC 2 cut(s) 46, 409
Asp700I GAANNNNTTC 1 cut(s) 1212
AspLEI GCGC 3 cut(s) 9, 29, 318
AspS9I GGNCC 1 cut(s) 93
AsuHPI GGTGA 3 cut(s) 867, 899, 943
AvaII GGWCC 1 cut(s) 93
BaeGI GKGCMC 1 cut(s) 119
BanI GGYRCC 1 cut(s) 116
BbsI GAAGAC 3 cut(s) 19, 314, 846
Bbv12I GWGCWC 1 cut(s) 353
BbvCI CCTCAGC 1 cut(s) 774
BbvI GCAGC 2 cut(s) 58, 421
BccI CCATC 3 cut(s) 231, 962, 1000
BceAI ACGGC 5 cut(s) 14, 54, 145, 184, 850
BciT130I CCWGG 2 cut(s) 18, 1025
BciVI GTATCC 1 cut(s) 511
BcoDI GTCTC 3 cut(s) 181, 221, 1024
BfaI CTAG 3 cut(s) 242, 468, 576
BfoI RGCGCY 1 cut(s) 319
BfrI CTTAAG 1 cut(s) 440
BfuI GTATCC 1 cut(s) 511
BisI GCNGC 6 cut(s) 47, 67, 133, 186, 369, 410
BlsI GCNGC 6 cut(s) 48, 68, 134, 187, 370, 411
Bme1390I CCNGG 2 cut(s) 18, 1025
Bme18I GGWCC 1 cut(s) 93
BmgT120I GGNCC 1 cut(s) 93
BmiI GGNNCC 4 cut(s) 38, 118, 387, 1095
BmrFI CCNGG 2 cut(s) 18, 1025
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 5 cut(s) 513, 546, 747, 760, 814
BmuI ACTGGG 1 cut(s) 229
BpiI GAAGAC 3 cut(s) 19, 314, 846
Bpu10I CCTNAGC 1 cut(s) 774
BpuEI CTTGAG 1 cut(s) 1010
BsaBI GATNNNNATC 1 cut(s) 489
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 4 cut(s) 544, 636, 1023, 1097
BsaXI ACNNNNNCTCC 4 cut(s) 6, 36, 377, 407
Bsc4I CCNNNNNNNGG 6 cut(s) 108, 111, 237, 375, 382, 917
Bse118I RCCGGY 1 cut(s) 388
Bse1I ACTGG 2 cut(s) 95, 224
Bse3DI GCAATG 1 cut(s) 418
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 2 cut(s) 18, 1025
BseDI CCNNGG 4 cut(s) 544, 636, 1023, 1097
BseGI GGATG 4 cut(s) 152, 724, 738, 1171
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 6 cut(s) 108, 111, 237, 375, 382, 917
BseMI GCAATG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 645, 765
BseNI ACTGG 2 cut(s) 95, 224
BseSI GKGCMC 1 cut(s) 119
BseX3I CGGCCG 1 cut(s) 130
BseXI GCAGC 2 cut(s) 58, 421
BseYI CCCAGC 1 cut(s) 206
Bsh1236I CGCG 2 cut(s) 27, 135
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 3 cut(s) 132, 368, 543
BshNI GGYRCC 1 cut(s) 116
BsiEI CGRYCG 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 3 cut(s) 43, 376, 389
BslFI GGGAC 1 cut(s) 878
BslI CCNNNNNNNGG 6 cut(s) 108, 111, 237, 375, 382, 917
BsmAI GTCTC 3 cut(s) 181, 221, 1024
BsmBI CGTCTC 1 cut(s) 181
BsmFI GGGAC 1 cut(s) 878
BsnI GGCC 3 cut(s) 132, 368, 543
Bso31I GGTCTC 1 cut(s) 221
Bsp1286I GDGCHC 2 cut(s) 119, 353
Bsp143I GATC 5 cut(s) 616, 627, 813, 913, 1101
Bsp19I CCATGG 1 cut(s) 544
BspACI CCGC 8 cut(s) 25, 67, 133, 145, 186, 195, 369, 526
BspANI GGCC 3 cut(s) 132, 368, 543
BspCNI CTCAG 2 cut(s) 644, 766
BspFNI CGCG 2 cut(s) 27, 135
BspHI TCATGA 1 cut(s) 964
BspLI GGNNCC 4 cut(s) 38, 118, 387, 1095
BspPI GGATC 2 cut(s) 611, 1109
BspT107I GGYRCC 1 cut(s) 116
BspTI CTTAAG 1 cut(s) 440
BspTNI GGTCTC 1 cut(s) 221
BsrBI CCGCTC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 388
BsrI ACTGG 2 cut(s) 95, 224
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 4 cut(s) 544, 636, 1023, 1097
BssMI GATC 5 cut(s) 616, 627, 813, 913, 1101
BssNI GRCGYC 1 cut(s) 171
BssT1I CCWWGG 2 cut(s) 544, 1097
Bst2UI CCWGG 2 cut(s) 18, 1025
Bst4CI ACNGT 5 cut(s) 355, 879, 925, 1087, 1148
Bst6I CTCTTC 1 cut(s) 242
BstACI GRCGYC 1 cut(s) 171
BstAFI CTTAAG 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 1000
BstC8I GCNNGC 5 cut(s) 212, 390, 560, 689, 693
BstDEI CTNAG 2 cut(s) 631, 774
BstDSI CCRYGG 1 cut(s) 544
BstF5I GGATG 4 cut(s) 152, 724, 738, 1171
BstFNI CGCG 2 cut(s) 27, 135
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 3 cut(s) 9, 29, 318
BstKTI GATC 5 cut(s) 619, 630, 816, 916, 1104
BstMAI GTCTC 3 cut(s) 181, 221, 1024
BstMBI GATC 5 cut(s) 616, 627, 813, 913, 1101
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 5 cut(s) 194, 385, 398, 473, 1000
BstNI CCWGG 2 cut(s) 18, 1025
BstSCI CCNGG 2 cut(s) 16, 1023
BstSLI GKGCMC 1 cut(s) 119
BstUI CGCG 2 cut(s) 27, 135
BstV1I GCAGC 2 cut(s) 58, 421
BstV2I GAAGAC 3 cut(s) 19, 314, 846
BstX2I RGATCY 1 cut(s) 1101
BstYI RGATCY 1 cut(s) 1101
BstZI CGGCCG 1 cut(s) 130
BsuI GTATCC 1 cut(s) 511
BsuRI GGCC 3 cut(s) 132, 368, 543
BtgI CCRYGG 1 cut(s) 544
BtsCI GGATG 4 cut(s) 152, 724, 738, 1171
BtsIMutI CAGTG 4 cut(s) 75, 217, 351, 1153
Cac8I GCNNGC 5 cut(s) 212, 390, 560, 689, 693
CaiI CAGNNNCTG 1 cut(s) 773
CciI TCATGA 1 cut(s) 964
CfoI GCGC 3 cut(s) 9, 29, 318
Cfr10I RCCGGY 1 cut(s) 388
Cfr13I GGNCC 1 cut(s) 93
CseI GACGC 2 cut(s) 124, 160
Csp6I GTAC 2 cut(s) 253, 1153
CviAII CATG 7 cut(s) 275, 545, 743, 917, 965, 1186, 1233
CviQI GTAC 2 cut(s) 253, 1153
DdeI CTNAG 2 cut(s) 631, 774
DpnI GATC 5 cut(s) 618, 629, 815, 915, 1103
DpnII GATC 5 cut(s) 616, 627, 813, 913, 1101
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 3 cut(s) 130, 366, 541
EagI CGGCCG 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
EclXI CGGCCG 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 544, 1097
Eco31I GGTCTC 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 93
Eco52I CGGCCG 1 cut(s) 130
Eco57I CTGAAG 1 cut(s) 515
EcoRII CCWGG 2 cut(s) 16, 1023
EcoT14I CCWWGG 2 cut(s) 544, 1097
EcoT22I ATGCAT 1 cut(s) 539
ErhI CCWWGG 2 cut(s) 544, 1097
Esp3I CGTCTC 1 cut(s) 181
FaeI CATG 7 cut(s) 278, 548, 746, 920, 968, 1189, 1236
FaqI GGGAC 1 cut(s) 878
FatI CATG 7 cut(s) 274, 544, 742, 916, 964, 1185, 1232
FauI CCCGC 2 cut(s) 202, 519
Fnu4HI GCNGC 6 cut(s) 47, 67, 133, 186, 369, 410
FokI GGATG 4 cut(s) 139, 725, 731, 1178
Fsp4HI GCNGC 6 cut(s) 47, 67, 133, 186, 369, 410
FspBI CTAG 3 cut(s) 242, 468, 576
GlaI GCGC 3 cut(s) 8, 28, 317
GluI GCNGC 6 cut(s) 47, 67, 133, 186, 369, 410
GsaI CCCAGC 1 cut(s) 210
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 3 cut(s) 132, 368, 543
HapII CCGG 3 cut(s) 43, 376, 389
HgaI GACGC 2 cut(s) 124, 160
HhaI GCGC 3 cut(s) 9, 29, 318
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 7 cut(s) 278, 548, 746, 920, 968, 1189, 1236
Hin6I GCGC 3 cut(s) 7, 27, 316
HinP1I GCGC 3 cut(s) 7, 27, 316
HindIII AAGCTT 2 cut(s) 212, 888
HinfI GANTC 8 cut(s) 21, 122, 269, 676, 764, 872, 961, 1067
HpaII CCGG 3 cut(s) 43, 376, 389
HphI GGTGA 3 cut(s) 867, 899, 943
Hpy166II GTNNAC 4 cut(s) 581, 934, 1083, 1155
Hpy188I TCNGA 9 cut(s) 36, 106, 127, 157, 296, 385, 495, 769, 946
Hpy188III TCNNGA 3 cut(s) 965, 989, 1064
Hpy8I GTNNAC 4 cut(s) 581, 934, 1083, 1155
Hpy99I CGWCG 5 cut(s) 131, 140, 176, 185, 338
HpyAV CCTTC 4 cut(s) 493, 508, 947, 990
HpyCH4III ACNGT 5 cut(s) 355, 879, 925, 1087, 1148
HpyCH4V TGCA 6 cut(s) 409, 537, 1107, 1128, 1177, 1240
HpyF10VI GCNNNNNNNGC 5 cut(s) 194, 385, 398, 473, 1000
HpyF3I CTNAG 2 cut(s) 631, 774
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 7 cut(s) 278, 548, 746, 920, 968, 1189, 1236
HspAI GCGC 3 cut(s) 7, 27, 316
KroI GCCGGC 1 cut(s) 388
KroNI GCCGGC 1 cut(s) 390
Kzo9I GATC 5 cut(s) 616, 627, 813, 913, 1101
LmnI GCTCC 3 cut(s) 152, 385, 555
Lsp1109I GCAGC 2 cut(s) 58, 421
LweI GCATC 5 cut(s) 513, 546, 747, 760, 814
MaeI CTAG 3 cut(s) 242, 468, 576
MalI GATC 5 cut(s) 618, 629, 815, 915, 1103
MbiI CCGCTC 1 cut(s) 147
MboI GATC 5 cut(s) 616, 627, 813, 913, 1101
MboII GAAGA 6 cut(s) 24, 259, 314, 508, 808, 851
MflI RGATCY 1 cut(s) 1101
MhlI GDGCHC 2 cut(s) 119, 353
MluCI AATT 1 cut(s) 612
MlyI GAGTC 1 cut(s) 15
MmeI TCCRAC 2 cut(s) 150, 283
Mph1103I ATGCAT 1 cut(s) 539
MroNI GCCGGC 1 cut(s) 388
MroXI GAANNNNTTC 1 cut(s) 1212
MseI TTAA 5 cut(s) 330, 441, 893, 1143, 1273
MslI CAYNNNNRTG 1 cut(s) 1231
MspCI CTTAAG 1 cut(s) 440
MspI CCGG 3 cut(s) 43, 376, 389
MspR9I CCNGG 2 cut(s) 18, 1025
MvaI CCWGG 2 cut(s) 18, 1025
MvnI CGCG 2 cut(s) 27, 135
MwoI GCNNNNNNNGC 5 cut(s) 194, 385, 398, 473, 1000
NaeI GCCGGC 1 cut(s) 390
NcoI CCATGG 1 cut(s) 544
NdeII GATC 5 cut(s) 616, 627, 813, 913, 1101
NgoMIV GCCGGC 1 cut(s) 388
NlaIII CATG 7 cut(s) 278, 548, 746, 920, 968, 1189, 1236
NlaIV GGNNCC 4 cut(s) 38, 118, 387, 1095
NsiI ATGCAT 1 cut(s) 539
PagI TCATGA 1 cut(s) 964
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 389
PdiI GCCGGC 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 1212
PfeI GAWTC 7 cut(s) 122, 269, 676, 764, 872, 961, 1067
PkrI GCNGC 6 cut(s) 48, 68, 134, 187, 370, 411
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Psp6I CCWGG 2 cut(s) 16, 1023
PspFI CCCAGC 1 cut(s) 206
PspGI CCWGG 2 cut(s) 16, 1023
PspN4I GGNNCC 4 cut(s) 38, 118, 387, 1095
PspPI GGNCC 1 cut(s) 93
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 1 cut(s) 1101
RsaI GTAC 2 cut(s) 254, 1154
RsaNI GTAC 2 cut(s) 253, 1153
RseI CAYNNNNRTG 1 cut(s) 1231
SaqAI TTAA 5 cut(s) 330, 441, 893, 1143, 1273
SatI GCNGC 6 cut(s) 47, 67, 133, 186, 369, 410
Sau3AI GATC 5 cut(s) 616, 627, 813, 913, 1101
Sau96I GGNCC 1 cut(s) 93
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 2 cut(s) 18, 1025
SduI GDGCHC 2 cut(s) 119, 353
SfaNI GCATC 5 cut(s) 513, 546, 747, 760, 814
SinI GGWCC 1 cut(s) 93
SmiMI CAYNNNNRTG 1 cut(s) 1231
SmlI CTYRAG 2 cut(s) 440, 989
SmoI CTYRAG 2 cut(s) 440, 989
Sse9I AATT 1 cut(s) 612
SsiI CCGC 8 cut(s) 25, 67, 133, 145, 186, 195, 369, 526
SspI AATATT 1 cut(s) 1213
SspMI CTAG 3 cut(s) 242, 468, 576
StyD4I CCNGG 2 cut(s) 16, 1023
StyI CCWWGG 2 cut(s) 544, 1097
TaaI ACNGT 5 cut(s) 355, 879, 925, 1087, 1148
TaqI TCGA 1 cut(s) 1063
TaqII GACCGA 1 cut(s) 245
TasI AATT 1 cut(s) 612
TauI GCSGC 4 cut(s) 69, 135, 188, 371
TfiI GAWTC 7 cut(s) 122, 269, 676, 764, 872, 961, 1067
Tru1I TTAA 5 cut(s) 330, 441, 893, 1143, 1273
Tru9I TTAA 5 cut(s) 330, 441, 893, 1143, 1273
TscAI CASTG 4 cut(s) 82, 224, 358, 1153
TseI GCWGC 2 cut(s) 46, 409
TspDTI ATGAA 7 cut(s) 324, 689, 772, 777, 808, 899, 929
TspGWI ACGGA 2 cut(s) 169, 669
TspRI CASTG 4 cut(s) 82, 224, 358, 1153
Vha464I CTTAAG 1 cut(s) 440
VpaK11BI GGWCC 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 1212
XspI CTAG 3 cut(s) 242, 468, 576
Zsp2I ATGCAT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.