pycom13g19570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006079
Physical Location & Seq
Forward (+)
15535632 .. 15537362
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g19570.2

Sequence Viewer

Length: 774 bp
ATGGGATCCACATTCCCCATTTCTAAAGCCCTCAGCAGCCGGCTTCACCCGGATCATGGCACAGTTGTGAACCTTCATTCAGATTCTGATATCTCGGACCCTGACGGCTCTCCTCCATCCGTTCTGCAGCAAACTTTGCCGATGGGGAAGTACGTGTCCAGGGTTTGCCGGGCATACAAGGATGTGGTAGTGGGAGGGAAAGATTCTGGTTTGGGGTTTACAGTTTCAGTCCCGTGGGTGTGCAATTCGTCTCTGGTCGTATGCAGAGGTTTTGGGGTTAAGGGTGGGGATAGCAACAGCGATGTAATCACGGACGCTCTGATATCCAAACAGAGCACAAGGAAATCAAAGAATGGCGTCAGAGGGGAGAAAGCGACTGTCGCTCCGAAGCAACGGCGAAGAACAGAGCATGCTGTCGAGGGTGTCGCAATCGGAGATCGTGCAGCAGACGAAGCGCGTGGCGATCGACGCGTCGTTGGTGACGAAGAAGCTGGCGAAGAGCACGGGGAAGGCGGCGTGGATATTGGGAACGACGTTCCTGATCTTGGGGGTCCCACTCATCATCGCCATGGATCGCGAGCAGCAGTTGAACGAGTTGGAGTTGCAGCAGGCCAGCATCCTCGGTTCTCCTCCCCCTCCCCCCGCTTTCAACTAAAAACGCAAACCCTGACCTCCGCCTTCCCTCAGCTCCTCACGGAGGCTCTTCCTCTCCGTTGGTCGCCTTTTCTTTTTCACTTTTTGTTTGTGTTAAATTGTAGTTTTTATGGATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

27.5

Weight (kDa)

8.3

Isoelectric Point (pI)

43.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 457, 471, 577
AciI CCGC 3 cut(s) 513, 643, 675
AclWI GGATC 3 cut(s) 13, 60, 580
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 3 cut(s) 56, 545, 697
AflIII ACRYGT 2 cut(s) 153, 469
AgsI TTSAA 2 cut(s) 590, 650
AjnI CCWGG 1 cut(s) 158
AleI CACNNNNGTG 1 cut(s) 65
AluBI AGCT 2 cut(s) 491, 688
AluI AGCT 2 cut(s) 491, 688
Alw21I GWGCWC 2 cut(s) 338, 504
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 3 cut(s) 13, 60, 580
AlwNI CAGNNNCTG 1 cut(s) 86
AoxI GGCC 1 cut(s) 610
ApeKI GCWGC 5 cut(s) 36, 127, 443, 581, 605
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 2 cut(s) 97, 551
AsuC2I CCSGG 2 cut(s) 50, 170
AsuHPI GGTGA 2 cut(s) 38, 491
AvaII GGWCC 2 cut(s) 97, 551
BamHI GGATCC 1 cut(s) 5
Bbv12I GWGCWC 2 cut(s) 338, 504
BbvCI CCTCAGC 2 cut(s) 32, 684
BbvI GCAGC 5 cut(s) 48, 139, 455, 593, 617
BccI CCATC 2 cut(s) 124, 136
BceAI ACGGC 2 cut(s) 121, 410
BciT130I CCWGG 1 cut(s) 160
BcnI CCSGG 2 cut(s) 50, 170
BcoDI GTCTC 1 cut(s) 255
BfmI CTRYAG 1 cut(s) 125
BisI GCNGC 6 cut(s) 37, 128, 444, 514, 582, 606
BlsI GCNGC 6 cut(s) 38, 129, 445, 515, 583, 607
Bme1390I CCNGG 3 cut(s) 50, 160, 170
Bme18I GGWCC 2 cut(s) 97, 551
BmgT120I GGNCC 2 cut(s) 97, 551
BmiI GGNNCC 4 cut(s) 7, 99, 552, 553
BmrFI CCNGG 3 cut(s) 50, 160, 170
BmsI GCATC 1 cut(s) 625
Bpu10I CCTNAGC 2 cut(s) 32, 684
BpuMI CCSGG 2 cut(s) 50, 170
BsaAI YACGTR 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 4 cut(s) 159, 233, 568, 620
BsaXI ACNNNNNCTCC 2 cut(s) 367, 397
Bsc4I CCNNNNNNNGG 3 cut(s) 56, 545, 697
Bse118I RCCGGY 1 cut(s) 39
BseBI CCWGG 1 cut(s) 160
BseDI CCNNGG 4 cut(s) 159, 233, 568, 620
BseGI GGATG 4 cut(s) 116, 187, 616, 773
BseLI CCNNNNNNNGG 3 cut(s) 56, 545, 697
BseMII CTCAG 2 cut(s) 46, 698
BseRI GAGGAG 3 cut(s) 102, 619, 680
BseXI GCAGC 5 cut(s) 48, 139, 455, 593, 617
BsgI GTGCAG 1 cut(s) 462
Bsh1236I CGCG 3 cut(s) 457, 471, 577
Bsh1285I CGRYCG 1 cut(s) 466
BshFI GGCC 1 cut(s) 612
BsiEI CGRYCG 1 cut(s) 466
BsiHKAI GWGCWC 2 cut(s) 338, 504
BsiSI CCGG 3 cut(s) 40, 50, 169
BslFI GGGAC 2 cut(s) 215, 537
BslI CCNNNNNNNGG 3 cut(s) 56, 545, 697
BsmAI GTCTC 1 cut(s) 255
BsmBI CGTCTC 1 cut(s) 255
BsmFI GGGAC 2 cut(s) 215, 537
BsnI GGCC 1 cut(s) 612
Bsp1286I GDGCHC 2 cut(s) 338, 504
Bsp143I GATC 6 cut(s) 5, 52, 436, 463, 541, 572
Bsp19I CCATGG 1 cut(s) 568
Bsp68I TCGCGA 1 cut(s) 577
BspACI CCGC 3 cut(s) 513, 643, 675
BspANI GGCC 1 cut(s) 612
BspCNI CTCAG 2 cut(s) 45, 697
BspFNI CGCG 3 cut(s) 457, 471, 577
BspLI GGNNCC 4 cut(s) 7, 99, 552, 553
BspMAI CTGCAG 1 cut(s) 129
BspPI GGATC 3 cut(s) 13, 60, 580
BspQI GCTCTTC 2 cut(s) 492, 708
BsrFI RCCGGY 1 cut(s) 39
BssAI RCCGGY 1 cut(s) 39
BssECI CCNNGG 4 cut(s) 159, 233, 568, 620
BssMI GATC 6 cut(s) 5, 52, 436, 463, 541, 572
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 568
Bst2UI CCWGG 1 cut(s) 160
Bst4CI ACNGT 3 cut(s) 64, 223, 379
Bst6I CTCTTC 2 cut(s) 492, 708
BstACI GRCGYC 1 cut(s) 357
BstAPI GCANNNNNTGC 1 cut(s) 136
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 6 cut(s) 41, 411, 493, 579, 610, 614
BstDEI CTNAG 2 cut(s) 32, 684
BstDSI CCRYGG 2 cut(s) 233, 568
BstENI CCTNNNNNAGG 1 cut(s) 695
BstF5I GGATG 4 cut(s) 116, 187, 616, 773
BstFNI CGCG 3 cut(s) 457, 471, 577
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 6 cut(s) 8, 55, 439, 466, 544, 575
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 6 cut(s) 5, 52, 436, 463, 541, 572
BstMCI CGRYCG 1 cut(s) 466
BstMWI GCNNNNNNNGC 4 cut(s) 136, 380, 452, 468
BstNI CCWGG 1 cut(s) 160
BstNSI RCATGY 1 cut(s) 413
BstSCI CCNGG 3 cut(s) 48, 158, 168
BstSFI CTRYAG 1 cut(s) 125
BstUI CGCG 3 cut(s) 457, 471, 577
BstV1I GCAGC 5 cut(s) 48, 139, 455, 593, 617
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
BsuRI GGCC 1 cut(s) 612
BtgI CCRYGG 2 cut(s) 233, 568
BtgZI GCGATG 2 cut(s) 315, 548
BtsCI GGATG 4 cut(s) 116, 187, 616, 773
BtuMI TCGCGA 1 cut(s) 577
Cac8I GCNNGC 6 cut(s) 41, 411, 493, 579, 610, 614
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 457
Cfr10I RCCGGY 1 cut(s) 39
Cfr13I GGNCC 2 cut(s) 97, 551
CseI GACGC 4 cut(s) 323, 346, 460, 477
Csp6I GTAC 1 cut(s) 151
CviAII CATG 3 cut(s) 56, 410, 569
CviJI RGCY 8 cut(s) 29, 39, 43, 108, 491, 612, 688, 701
CviKI_1 RGCY 8 cut(s) 29, 39, 43, 108, 491, 612, 688, 701
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 2 cut(s) 32, 684
DpnI GATC 6 cut(s) 7, 54, 438, 465, 543, 574
DpnII GATC 6 cut(s) 5, 52, 436, 463, 541, 572
Eam1104I CTCTTC 2 cut(s) 492, 708
EarI CTCTTC 2 cut(s) 492, 708
EciI GGCGGA 1 cut(s) 664
Eco130I CCWWGG 1 cut(s) 568
Eco32I GATATC 2 cut(s) 91, 324
Eco47I GGWCC 2 cut(s) 97, 551
EcoNI CCTNNNNNAGG 1 cut(s) 695
EcoO109I RGGNCCY 1 cut(s) 551
EcoRII CCWGG 1 cut(s) 158
EcoRV GATATC 2 cut(s) 91, 324
EcoT14I CCWWGG 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 568
Esp3I CGTCTC 1 cut(s) 255
FaeI CATG 3 cut(s) 59, 413, 572
FaiI YATR 6 cut(s) 57, 175, 262, 411, 570, 765
FaqI GGGAC 2 cut(s) 215, 537
FatI CATG 3 cut(s) 55, 409, 568
FauI CCCGC 1 cut(s) 650
Fnu4HI GCNGC 6 cut(s) 37, 128, 444, 514, 582, 606
FokI GGATG 3 cut(s) 103, 194, 603
Fsp4HI GCNGC 6 cut(s) 37, 128, 444, 514, 582, 606
GlaI GCGC 1 cut(s) 456
GluI GCNGC 6 cut(s) 37, 128, 444, 514, 582, 606
HaeIII GGCC 1 cut(s) 612
HapII CCGG 3 cut(s) 40, 50, 169
HgaI GACGC 4 cut(s) 323, 346, 460, 477
HhaI GCGC 1 cut(s) 457
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 3 cut(s) 59, 413, 572
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HinfI GANTC 2 cut(s) 83, 203
HpaII CCGG 3 cut(s) 40, 50, 169
HphI GGTGA 2 cut(s) 38, 491
Hpy166II GTNNAC 2 cut(s) 70, 219
Hpy188I TCNGA 7 cut(s) 82, 88, 97, 321, 362, 387, 434
Hpy188III TCNNGA 2 cut(s) 539, 576
Hpy8I GTNNAC 2 cut(s) 70, 219
Hpy99I CGWCG 3 cut(s) 471, 476, 536
HpyAV CCTTC 3 cut(s) 83, 503, 688
HpyCH4III ACNGT 3 cut(s) 64, 223, 379
HpyCH4IV ACGT 2 cut(s) 153, 534
HpyCH4V TGCA 5 cut(s) 127, 243, 264, 443, 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 136, 380, 452, 468
HpyF3I CTNAG 2 cut(s) 32, 684
HpySE526I ACGT 2 cut(s) 153, 534
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 3 cut(s) 59, 413, 572
HspAI GCGC 1 cut(s) 455
KflI GGGWCCC 1 cut(s) 551
KroI GCCGGC 1 cut(s) 39
KroNI GCCGGC 1 cut(s) 41
Kzo9I GATC 6 cut(s) 5, 52, 436, 463, 541, 572
LguI GCTCTTC 2 cut(s) 492, 708
LmnI GCTCC 2 cut(s) 388, 693
Lsp1109I GCAGC 5 cut(s) 48, 139, 455, 593, 617
LweI GCATC 1 cut(s) 625
MaeII ACGT 2 cut(s) 153, 534
MaeIII GTNAC 1 cut(s) 479
MalI GATC 6 cut(s) 7, 54, 438, 465, 543, 574
MboI GATC 6 cut(s) 5, 52, 436, 463, 541, 572
MboII GAAGA 4 cut(s) 411, 497, 509, 695
MflI RGATCY 1 cut(s) 5
MhlI GDGCHC 2 cut(s) 338, 504
MluCI AATT 2 cut(s) 244, 751
MluI ACGCGT 1 cut(s) 469
MmeI TCCRAC 1 cut(s) 577
MroNI GCCGGC 1 cut(s) 39
MseI TTAA 2 cut(s) 279, 749
MslI CAYNNNNRTG 2 cut(s) 65, 567
MspI CCGG 3 cut(s) 40, 50, 169
MspR9I CCNGG 3 cut(s) 50, 160, 170
MvaI CCWGG 1 cut(s) 160
MvnI CGCG 3 cut(s) 457, 471, 577
MwoI GCNNNNNNNGC 4 cut(s) 136, 380, 452, 468
NaeI GCCGGC 1 cut(s) 41
NciI CCSGG 2 cut(s) 50, 170
NcoI CCATGG 1 cut(s) 568
NdeII GATC 6 cut(s) 5, 52, 436, 463, 541, 572
NgoMIV GCCGGC 1 cut(s) 39
NlaIII CATG 3 cut(s) 59, 413, 572
NlaIV GGNNCC 4 cut(s) 7, 99, 552, 553
NmuCI GTSAC 1 cut(s) 479
NruI TCGCGA 1 cut(s) 577
NspI RCATGY 1 cut(s) 413
OliI CACNNNNGTG 1 cut(s) 65
PaeI GCATGC 1 cut(s) 413
PciSI GCTCTTC 2 cut(s) 492, 708
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PdiI GCCGGC 1 cut(s) 41
PfeI GAWTC 2 cut(s) 83, 203
PkrI GCNGC 6 cut(s) 38, 129, 445, 515, 583, 607
Ple19I CGATCG 1 cut(s) 466
Ppu21I YACGTR 1 cut(s) 154
PpuMI RGGWCCY 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 551
Psp6I CCWGG 1 cut(s) 158
PspGI CCWGG 1 cut(s) 158
PspN4I GGNNCC 4 cut(s) 7, 99, 552, 553
PspPI GGNCC 2 cut(s) 97, 551
PspPPI RGGWCCY 1 cut(s) 551
PstI CTGCAG 1 cut(s) 129
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 5
PvuI CGATCG 1 cut(s) 466
RruI TCGCGA 1 cut(s) 577
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 2 cut(s) 65, 567
SapI GCTCTTC 2 cut(s) 492, 708
SaqAI TTAA 2 cut(s) 279, 749
SatI GCNGC 6 cut(s) 37, 128, 444, 514, 582, 606
Sau3AI GATC 6 cut(s) 5, 52, 436, 463, 541, 572
Sau96I GGNCC 2 cut(s) 97, 551
ScrFI CCNGG 3 cut(s) 50, 160, 170
SduI GDGCHC 2 cut(s) 338, 504
SetI ASST 7 cut(s) 75, 156, 271, 493, 537, 674, 690
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 1 cut(s) 125
SinI GGWCC 2 cut(s) 97, 551
SmiMI CAYNNNNRTG 2 cut(s) 65, 567
SphI GCATGC 1 cut(s) 413
Sse9I AATT 2 cut(s) 244, 751
SsiI CCGC 3 cut(s) 513, 643, 675
StyD4I CCNGG 3 cut(s) 48, 158, 168
StyI CCWWGG 1 cut(s) 568
TaaI ACNGT 3 cut(s) 64, 223, 379
TaiI ACGT 2 cut(s) 156, 537
TaqI TCGA 2 cut(s) 417, 466
TasI AATT 2 cut(s) 244, 751
TauI GCSGC 1 cut(s) 516
TfiI GAWTC 2 cut(s) 83, 203
Tru1I TTAA 2 cut(s) 279, 749
Tru9I TTAA 2 cut(s) 279, 749
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 5 cut(s) 36, 127, 443, 581, 605
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 1 cut(s) 65
TspGWI ACGGA 4 cut(s) 109, 326, 701, 710
VpaK11BI GGWCC 2 cut(s) 97, 551
XagI CCTNNNNNAGG 1 cut(s) 695
XceI RCATGY 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.