Rroxscaffold_5G00340310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006079
Physical Location & Seq
Forward (+)
8766265 .. 8768015
1751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00340310.1

Sequence Viewer

Length: 1263 bp
ATGTGGCGCAGAAGAGCTGGATGGAACATCCGGAAGTTCTCAACGGCAATACGCCGCCGTGTTAACGACGAGGGCGACTGGTCCTACGCCTCCGAATGGTGGGGCACCGAATCGGACGGCCGCATCGTCCTCCGCTCCACATCCGACAAGGGAAACGGCGTCGTCTCCGTCGCCGCTTTCCCTGCCTCCAATCCCAGCAAGCTTCACTGGGCAGCAACAGAGAGATGGCTAGAGCAAAGGTACGCAGAGATAAACAAAACCCAGTGTTACAATGAGAAGTTCAGAGTGCTTGGATATGAATGGCGCGCTCTTCGTTTTAACGACGACACCCGCCAAAGCACGGTCAAAATCTTGGCTGCTTTCCGGCAATCGGAGCCGGCCTCTTTTGCTCTTATGCAGCAACCTCATTGCCTCGCTGTTCCATATCTGAAGAGTATGTTATCAGTTGGGTTGGCTACTATAGCTTGTTGTGATTTCAATGTGAAGAAAGTGGCGACTGGGAAGGAAAAGATGAGTATTTTATGCATTGGCCATGGAGGAGGGAGCTTGCCATTGTTTTTGGCTAGTAAAATTCAAGGTGCTACGGTTGACATAGTTGAAATCGATCCTGTTGTAATCTCAGCCTCAGTTCAAGCTATGGGGTTTCCTGCTTACTCAGTTATGAATCCGTCAGGCAAGCGCGCTTTTTCAAAACCCGATATCATGGATGAGGTTCTGTGGAAAGGCATCCACGAGAGGCTGTTTCTCTATGAATCAGATGCTGAGGATTTCATTCTCAAAACCACCAATGTGTATGATATGATCTTCATTGATGCTTATGACGGGGAAGACATATTCCCTCGCAAGTTGTGGGACACAGACTCACCATTTATGAAAACTCTCAGCAGTCGGCTTCACCCCGATCATGGAACGGTTGTGGTGAACCTTCATTCAGATTCTGAGGTTTTGAATCCTGATGGCTCTGTCCCGTCCATTCTGGAGCAAGTTTTGCCAATGGGAAAGTACGTCTCCCAGGTTTGCCAAGCATACAAGGATGTGGTAGTGGGCAGTCGAGACTCTGGTTTGGGGTTTACTGTTTCCGTTCCTTGGATCTGCAACATGTCTATGGTTGTTTGCAGAGGTTTTGGGATTAAGGGTGGGTACACGAACAGGGATGTGATGTTGAATACTCTCATGTCCAAATCATTTGAAGTTAAAAATGTTCTCAACTTACCCTTCTCATGTTTGCAATATGTAAAGAGAGGTTTTAGACTTCTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

420

Amino Acids

47.2

Weight (kDa)

8.46

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccBSI CCGCTC 1 cut(s) 135
AccII CGCG 2 cut(s) 306, 681
AccIII TCCGGA 1 cut(s) 30
AciI CCGC 5 cut(s) 55, 121, 133, 174, 331
AclWI GGATC 2 cut(s) 599, 1097
AcoI YGGCCR 2 cut(s) 118, 529
AcsI RAATTY 1 cut(s) 570
AcuI CTGAAG 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 159
AfaI GTAC 3 cut(s) 242, 1004, 1142
AfiI CCNNNNNNNGG 6 cut(s) 96, 99, 340, 370, 905, 1086
AflIII ACRYGT 1 cut(s) 1098
AgsI TTSAA 8 cut(s) 478, 575, 599, 632, 690, 949, 1165, 1190
AjnI CCWGG 1 cut(s) 1011
AleI CACNNNNGTG 1 cut(s) 788
AluBI AGCT 5 cut(s) 17, 202, 464, 546, 635
AluI AGCT 5 cut(s) 17, 202, 464, 546, 635
Alw26I GTCTC 3 cut(s) 169, 1012, 1047
AlwI GGATC 2 cut(s) 599, 1097
AlwNI CAGNNNCTG 2 cut(s) 761, 938
Aor13HI TCCGGA 1 cut(s) 30
AoxI GGCC 3 cut(s) 118, 378, 529
ApeKI GCWGC 3 cut(s) 212, 356, 397
ApoI RAATTY 1 cut(s) 570
AspLEI GCGC 5 cut(s) 9, 306, 308, 681, 683
AspS9I GGNCC 1 cut(s) 81
AsuHPI GGTGA 3 cut(s) 855, 887, 931
AvaII GGWCC 1 cut(s) 81
BaeGI GKGCMC 1 cut(s) 107
BalI TGGCCA 1 cut(s) 531
BanI GGYRCC 1 cut(s) 104
BauI CACGAG 1 cut(s) 731
BbsI GAAGAC 1 cut(s) 834
BbvCI CCTCAGC 1 cut(s) 762
BbvI GCAGC 3 cut(s) 224, 343, 409
BccI CCATC 3 cut(s) 15, 219, 950
BceAI ACGGC 4 cut(s) 42, 60, 133, 172
BciT130I CCWGG 1 cut(s) 1013
BcoDI GTCTC 3 cut(s) 169, 1012, 1047
BfaI CTAG 2 cut(s) 230, 564
BfmI CTRYAG 1 cut(s) 459
BisI GCNGC 6 cut(s) 55, 121, 174, 213, 357, 398
BlsI GCNGC 6 cut(s) 56, 122, 175, 214, 358, 399
Bme1390I CCNGG 1 cut(s) 1013
Bme18I GGWCC 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 106, 375
BmrFI CCNGG 1 cut(s) 1013
BmrI ACTGGG 3 cut(s) 217, 256, 507
BmsI GCATC 4 cut(s) 132, 735, 748, 802
BmuI ACTGGG 3 cut(s) 217, 256, 507
BpiI GAAGAC 1 cut(s) 834
BplI GAGNNNNNCTC 2 cut(s) 365, 397
BpmI CTGGAG 1 cut(s) 998
Bpu10I CCTNAGC 1 cut(s) 762
Bsa29I ATCGAT 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 3 cut(s) 532, 1011, 1085
BsaWI WCCGGW 1 cut(s) 30
Bsc4I CCNNNNNNNGG 6 cut(s) 96, 99, 340, 370, 905, 1086
Bse118I RCCGGY 1 cut(s) 376
Bse1I ACTGG 4 cut(s) 83, 212, 262, 502
Bse3DI GCAATG 1 cut(s) 406
BseAI TCCGGA 1 cut(s) 30
BseBI CCWGG 1 cut(s) 1013
BseCI ATCGAT 1 cut(s) 603
BseDI CCNNGG 3 cut(s) 532, 1011, 1085
BseGI GGATG 7 cut(s) 26, 27, 140, 712, 726, 1039, 1159
BseLI CCNNNNNNNGG 6 cut(s) 96, 99, 340, 370, 905, 1086
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 6 cut(s) 633, 639, 669, 753, 895, 930
BseNI ACTGG 4 cut(s) 83, 212, 262, 502
BsePI GCGCGC 2 cut(s) 304, 679
BseRI GAGGAG 1 cut(s) 552
BseSI GKGCMC 1 cut(s) 107
BseX3I CGGCCG 1 cut(s) 118
BseXI GCAGC 3 cut(s) 224, 343, 409
BseYI CCCAGC 1 cut(s) 194
Bsh1236I CGCG 2 cut(s) 306, 681
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 3 cut(s) 120, 380, 531
BshNI GGYRCC 1 cut(s) 104
BshVI ATCGAT 1 cut(s) 603
BsiEI CGRYCG 1 cut(s) 121
BsiSI CCGG 3 cut(s) 31, 364, 377
BslFI GGGAC 2 cut(s) 866, 950
BslI CCNNNNNNNGG 6 cut(s) 96, 99, 340, 370, 905, 1086
BsmAI GTCTC 3 cut(s) 169, 1012, 1047
BsmBI CGTCTC 2 cut(s) 169, 1012
BsmFI GGGAC 2 cut(s) 866, 950
BsnI GGCC 3 cut(s) 120, 380, 531
Bsp1286I GDGCHC 1 cut(s) 107
Bsp13I TCCGGA 1 cut(s) 30
Bsp143I GATC 4 cut(s) 604, 801, 901, 1089
Bsp19I CCATGG 1 cut(s) 532
BspACI CCGC 5 cut(s) 55, 121, 133, 174, 331
BspANI GGCC 3 cut(s) 120, 380, 531
BspCNI CTCAG 6 cut(s) 632, 638, 668, 754, 894, 931
BspDI ATCGAT 1 cut(s) 603
BspEI TCCGGA 1 cut(s) 30
BspFNI CGCG 2 cut(s) 306, 681
BspLI GGNNCC 2 cut(s) 106, 375
BspPI GGATC 2 cut(s) 599, 1097
BspQI GCTCTTC 2 cut(s) 7, 315
BspT107I GGYRCC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 135
BsrDI GCAATG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 376
BsrI ACTGG 4 cut(s) 83, 212, 262, 502
BssAI RCCGGY 1 cut(s) 376
BssECI CCNNGG 3 cut(s) 532, 1011, 1085
BssHII GCGCGC 2 cut(s) 304, 679
BssMI GATC 4 cut(s) 604, 801, 901, 1089
BssNI GRCGYC 1 cut(s) 159
BssSI CACGAG 1 cut(s) 731
BssT1I CCWWGG 2 cut(s) 532, 1085
Bst2BI CACGAG 1 cut(s) 731
Bst2UI CCWGG 1 cut(s) 1013
Bst4CI ACNGT 4 cut(s) 343, 586, 913, 1075
Bst6I CTCTTC 3 cut(s) 7, 315, 425
BstACI GRCGYC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 988
BstC8I GCNNGC 6 cut(s) 200, 306, 378, 548, 677, 681
BstDEI CTNAG 6 cut(s) 619, 625, 655, 762, 881, 939
BstDSI CCRYGG 1 cut(s) 532
BstF5I GGATG 7 cut(s) 26, 27, 140, 712, 726, 1039, 1159
BstFNI CGCG 2 cut(s) 306, 681
BstHHI GCGC 5 cut(s) 9, 306, 308, 681, 683
BstKTI GATC 4 cut(s) 607, 804, 904, 1092
BstMAI GTCTC 3 cut(s) 169, 1012, 1047
BstMBI GATC 4 cut(s) 604, 801, 901, 1089
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 5 cut(s) 182, 373, 386, 461, 988
BstNI CCWGG 1 cut(s) 1013
BstNSI RCATGY 1 cut(s) 1102
BstSCI CCNGG 1 cut(s) 1011
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 2 cut(s) 306, 681
BstV1I GCAGC 3 cut(s) 224, 343, 409
BstV2I GAAGAC 1 cut(s) 834
BstX2I RGATCY 1 cut(s) 1089
BstYI RGATCY 1 cut(s) 1089
BstZI CGGCCG 1 cut(s) 118
Bsu15I ATCGAT 1 cut(s) 603
BsuRI GGCC 3 cut(s) 120, 380, 531
BsuTUI ATCGAT 1 cut(s) 603
BtgI CCRYGG 1 cut(s) 532
BtsCI GGATG 7 cut(s) 26, 27, 140, 712, 726, 1039, 1159
BtsIMutI CAGTG 2 cut(s) 205, 269
Cac8I GCNNGC 6 cut(s) 200, 306, 378, 548, 677, 681
CaiI CAGNNNCTG 2 cut(s) 761, 938
CfoI GCGC 5 cut(s) 9, 306, 308, 681, 683
Cfr10I RCCGGY 1 cut(s) 376
Cfr13I GGNCC 1 cut(s) 81
ClaI ATCGAT 1 cut(s) 603
CseI GACGC 1 cut(s) 148
Csp6I GTAC 3 cut(s) 241, 1003, 1141
CviAII CATG 6 cut(s) 533, 703, 905, 1099, 1174, 1221
CviQI GTAC 3 cut(s) 241, 1003, 1141
DdeI CTNAG 6 cut(s) 619, 625, 655, 762, 881, 939
DpnI GATC 4 cut(s) 606, 803, 903, 1091
DpnII GATC 4 cut(s) 604, 801, 901, 1089
EaeI YGGCCR 2 cut(s) 118, 529
EagI CGGCCG 1 cut(s) 118
Eam1104I CTCTTC 3 cut(s) 7, 315, 425
EarI CTCTTC 3 cut(s) 7, 315, 425
EclXI CGGCCG 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 532, 1085
Eco32I GATATC 1 cut(s) 700
Eco47I GGWCC 1 cut(s) 81
Eco52I CGGCCG 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 1011
EcoRV GATATC 1 cut(s) 700
EcoT14I CCWWGG 2 cut(s) 532, 1085
EcoT22I ATGCAT 1 cut(s) 527
ErhI CCWWGG 2 cut(s) 532, 1085
Esp3I CGTCTC 2 cut(s) 169, 1012
FaeI CATG 6 cut(s) 536, 706, 908, 1102, 1177, 1224
FaqI GGGAC 2 cut(s) 866, 950
FatI CATG 6 cut(s) 532, 702, 904, 1098, 1173, 1220
FauI CCCGC 1 cut(s) 338
Fnu4HI GCNGC 6 cut(s) 55, 121, 174, 213, 357, 398
FokI GGATG 7 cut(s) 14, 33, 127, 713, 719, 1046, 1166
Fsp4HI GCNGC 6 cut(s) 55, 121, 174, 213, 357, 398
FspBI CTAG 2 cut(s) 230, 564
GlaI GCGC 5 cut(s) 8, 305, 307, 680, 682
GluI GCNGC 6 cut(s) 55, 121, 174, 213, 357, 398
GsaI CCCAGC 1 cut(s) 198
GsuI CTGGAG 1 cut(s) 998
HaeIII GGCC 3 cut(s) 120, 380, 531
HapII CCGG 3 cut(s) 31, 364, 377
HgaI GACGC 1 cut(s) 148
HhaI GCGC 5 cut(s) 9, 306, 308, 681, 683
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 6 cut(s) 536, 706, 908, 1102, 1177, 1224
Hin6I GCGC 5 cut(s) 7, 304, 306, 679, 681
HinP1I GCGC 5 cut(s) 7, 304, 306, 679, 681
HincII GTYRAC 2 cut(s) 64, 589
HindII GTYRAC 2 cut(s) 64, 589
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 7 cut(s) 110, 664, 752, 860, 935, 949, 1055
HpaI GTTAAC 1 cut(s) 64
HpaII CCGG 3 cut(s) 31, 364, 377
HphI GGTGA 3 cut(s) 855, 887, 931
Hpy166II GTNNAC 5 cut(s) 64, 589, 922, 1071, 1143
Hpy188I TCNGA 9 cut(s) 94, 115, 145, 284, 373, 429, 757, 934, 940
Hpy188III TCNNGA 5 cut(s) 31, 953, 977, 1052, 1256
Hpy8I GTNNAC 5 cut(s) 64, 589, 922, 1071, 1143
Hpy99I CGWCG 4 cut(s) 71, 164, 173, 326
HpyAV CCTTC 3 cut(s) 496, 935, 1225
HpyCH4III ACNGT 4 cut(s) 343, 586, 913, 1075
HpyCH4IV ACGT 1 cut(s) 1005
HpyCH4V TGCA 5 cut(s) 397, 525, 1095, 1116, 1228
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 373, 386, 461, 988
HpyF3I CTNAG 6 cut(s) 619, 625, 655, 762, 881, 939
HpySE526I ACGT 1 cut(s) 1005
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 6 cut(s) 536, 706, 908, 1102, 1177, 1224
HspAI GCGC 5 cut(s) 7, 304, 306, 679, 681
Kpn2I TCCGGA 1 cut(s) 30
KroI GCCGGC 1 cut(s) 376
KroNI GCCGGC 1 cut(s) 378
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 4 cut(s) 604, 801, 901, 1089
LguI GCTCTTC 2 cut(s) 7, 315
LmnI GCTCC 4 cut(s) 140, 373, 543, 979
Lsp1109I GCAGC 3 cut(s) 224, 343, 409
LweI GCATC 4 cut(s) 132, 735, 748, 802
MaeI CTAG 2 cut(s) 230, 564
MaeII ACGT 1 cut(s) 1005
MaeIII GTNAC 1 cut(s) 266
MalI GATC 4 cut(s) 606, 803, 903, 1091
MbiI CCGCTC 1 cut(s) 135
MboI GATC 4 cut(s) 604, 801, 901, 1089
MboII GAAGA 6 cut(s) 24, 302, 442, 496, 796, 839
MflI RGATCY 1 cut(s) 1089
MhlI GDGCHC 1 cut(s) 107
MlsI TGGCCA 1 cut(s) 531
MluCI AATT 1 cut(s) 570
MluNI TGGCCA 1 cut(s) 531
MlyI GAGTC 2 cut(s) 854, 1049
MmeI TCCRAC 1 cut(s) 168
Mox20I TGGCCA 1 cut(s) 531
Mph1103I ATGCAT 1 cut(s) 527
MroI TCCGGA 1 cut(s) 30
MroNI GCCGGC 1 cut(s) 376
MscI TGGCCA 1 cut(s) 531
MseI TTAA 5 cut(s) 63, 318, 1131, 1194, 1261
MslI CAYNNNNRTG 2 cut(s) 788, 1103
Msp20I TGGCCA 1 cut(s) 531
MspI CCGG 3 cut(s) 31, 364, 377
MspR9I CCNGG 1 cut(s) 1013
MvaI CCWGG 1 cut(s) 1013
MvnI CGCG 2 cut(s) 306, 681
MwoI GCNNNNNNNGC 5 cut(s) 182, 373, 386, 461, 988
NaeI GCCGGC 1 cut(s) 378
NcoI CCATGG 1 cut(s) 532
NdeII GATC 4 cut(s) 604, 801, 901, 1089
NgoMIV GCCGGC 1 cut(s) 376
NlaIII CATG 6 cut(s) 536, 706, 908, 1102, 1177, 1224
NlaIV GGNNCC 2 cut(s) 106, 375
NsiI ATGCAT 1 cut(s) 527
NspI RCATGY 1 cut(s) 1102
OliI CACNNNNGTG 1 cut(s) 788
PauI GCGCGC 2 cut(s) 304, 679
PciI ACATGT 1 cut(s) 1098
PciSI GCTCTTC 2 cut(s) 7, 315
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 123
PdiI GCCGGC 1 cut(s) 378
PfeI GAWTC 5 cut(s) 110, 664, 752, 935, 949
PkrI GCNGC 6 cut(s) 56, 122, 175, 214, 358, 399
PleI GAGTC 2 cut(s) 854, 1049
PpsI GAGTC 2 cut(s) 854, 1049
PscI ACATGT 1 cut(s) 1098
Psp6I CCWGG 1 cut(s) 1011
PspFI CCCAGC 1 cut(s) 194
PspGI CCWGG 1 cut(s) 1011
PspN4I GGNNCC 2 cut(s) 106, 375
PspPI GGNCC 1 cut(s) 81
PstNI CAGNNNCTG 2 cut(s) 761, 938
PsuI RGATCY 1 cut(s) 1089
PteI GCGCGC 2 cut(s) 304, 679
RsaI GTAC 3 cut(s) 242, 1004, 1142
RsaNI GTAC 3 cut(s) 241, 1003, 1141
RseI CAYNNNNRTG 2 cut(s) 788, 1103
SapI GCTCTTC 2 cut(s) 7, 315
SaqAI TTAA 5 cut(s) 63, 318, 1131, 1194, 1261
SatI GCNGC 6 cut(s) 55, 121, 174, 213, 357, 398
Sau3AI GATC 4 cut(s) 604, 801, 901, 1089
Sau96I GGNCC 1 cut(s) 81
SchI GAGTC 2 cut(s) 854, 1049
ScrFI CCNGG 1 cut(s) 1013
SduI GDGCHC 1 cut(s) 107
SfaNI GCATC 4 cut(s) 132, 735, 748, 802
SfcI CTRYAG 1 cut(s) 459
SinI GGWCC 1 cut(s) 81
SmiMI CAYNNNNRTG 2 cut(s) 788, 1103
Sse9I AATT 1 cut(s) 570
SsiI CCGC 5 cut(s) 55, 121, 133, 174, 331
SspMI CTAG 2 cut(s) 230, 564
StyD4I CCNGG 1 cut(s) 1011
StyI CCWWGG 2 cut(s) 532, 1085
TaaI ACNGT 4 cut(s) 343, 586, 913, 1075
TaiI ACGT 1 cut(s) 1008
TaqI TCGA 2 cut(s) 603, 1051
TasI AATT 1 cut(s) 570
TauI GCSGC 3 cut(s) 57, 123, 176
TfiI GAWTC 5 cut(s) 110, 664, 752, 935, 949
Tru1I TTAA 5 cut(s) 63, 318, 1131, 1194, 1261
Tru9I TTAA 5 cut(s) 63, 318, 1131, 1194, 1261
TscAI CASTG 2 cut(s) 212, 269
TseI GCWGC 3 cut(s) 212, 356, 397
TspDTI ATGAA 7 cut(s) 312, 677, 760, 765, 796, 887, 917
TspGWI ACGGA 3 cut(s) 157, 657, 1069
TspRI CASTG 2 cut(s) 212, 269
VpaK11BI GGWCC 1 cut(s) 81
XapI RAATTY 1 cut(s) 570
XceI RCATGY 1 cut(s) 1102
XspI CTAG 2 cut(s) 230, 564
Zsp2I ATGCAT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.