FvH4_4g05040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011146
Physical Location & Seq
Forward (+)
4359794 .. 4363482
3689 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05040.t1

Sequence Viewer

Length: 471 bp
ATGAATCACCAAAACAACAAAGGAATAGGAATGGAGGGACAGCAGCAATGGAGGCCCAGACTGTCGTTCAGGAGCGCCACCATAATGATGTGCTTGCTGAACCTCATCACTGCTCTTCTTTTGCTTCAGGGCTTTCTCTCATCTGCTTCTTCCCGCTCCAAATCCTCACCCACTTCAGCCACACTCAGGTATGTTAGAGAATCTGAAGAGATCCGTCTTGCTATGCAACCTTTGGAACTAATGAAAAGGGTGAGGGAAATCCAACGAGAAGCATCTGGAGAACCAGAAACAGGCCAAGAGAAAGAGACAAGGCAAACAGTTGCAGTTGACCTCTCAAAAAGATTGAAGGATCTGCATTCAGCTAACGATGCTAACAGCTTGAAAGCCTTGGAAGAGTGGCGTAAACGGAAGATGGAACGAGCAAGACTACGTGACCTGGAGAAAAATGGAACACTCACGTCTCAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.84

Weight (kDa)

10.06

Isoelectric Point (pI)

50.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 156
AciI CCGC 1 cut(s) 154
AclWI GGATC 2 cut(s) 205, 357
AcuI CTGAAG 3 cut(s) 110, 159, 225
AfiI CCNNNNNNNGG 2 cut(s) 186, 290
AgsI TTSAA 2 cut(s) 346, 382
AjiI CACGTC 1 cut(s) 459
AjnI CCWGG 1 cut(s) 435
AluBI AGCT 3 cut(s) 362, 378, 467
AluI AGCT 3 cut(s) 362, 378, 467
Alw26I GTCTC 2 cut(s) 299, 465
AlwI GGATC 2 cut(s) 205, 357
AoxI GGCC 2 cut(s) 53, 292
ApeKI GCWGC 1 cut(s) 43
AspLEI GCGC 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 2 cut(s) 159, 262
BbvI GCAGC 1 cut(s) 55
BccI CCATC 1 cut(s) 406
BciT130I CCWGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 299, 465
BfoI RGCGCY 1 cut(s) 78
BisI GCNGC 1 cut(s) 44
BlsI GCNGC 1 cut(s) 45
Bme1390I CCNGG 1 cut(s) 437
BmgBI CACGTC 1 cut(s) 459
BmgT120I GGNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 437
BmsI GCATC 2 cut(s) 281, 358
BpmI CTGGAG 2 cut(s) 297, 458
BpuEI CTTGAG 1 cut(s) 447
BsaAI YACGTR 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 387
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 290
Bse3DI GCAATG 1 cut(s) 53
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 1 cut(s) 387
BseLI CCNNNNNNNGG 2 cut(s) 186, 290
BseMI GCAATG 1 cut(s) 53
BseMII CTCAG 1 cut(s) 199
BseXI GCAGC 1 cut(s) 55
BshFI GGCC 2 cut(s) 55, 294
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 2 cut(s) 186, 290
BsmAI GTCTC 2 cut(s) 299, 465
BsmBI CGTCTC 1 cut(s) 465
BsmFI GGGAC 1 cut(s) 51
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 2 cut(s) 55, 294
Bsp143I GATC 2 cut(s) 210, 349
BspACI CCGC 1 cut(s) 154
BspANI GGCC 2 cut(s) 55, 294
BspCNI CTCAG 1 cut(s) 198
BspPI GGATC 2 cut(s) 205, 357
BspQI GCTCTTC 1 cut(s) 120
BsrBI CCGCTC 1 cut(s) 156
BsrDI GCAATG 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 2 cut(s) 210, 349
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 2 cut(s) 63, 319
Bst6I CTCTTC 3 cut(s) 120, 201, 387
BstBAI YACGTR 1 cut(s) 431
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 185
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 1 cut(s) 77
BstKTI GATC 2 cut(s) 213, 352
BstMAI GTCTC 2 cut(s) 299, 465
BstMBI GATC 2 cut(s) 210, 349
BstMWI GCNNNNNNNGC 2 cut(s) 52, 368
BstNI CCWGG 1 cut(s) 437
BstSCI CCNGG 1 cut(s) 435
BstV1I GCAGC 1 cut(s) 55
BstX2I RGATCY 2 cut(s) 210, 349
BstYI RGATCY 2 cut(s) 210, 349
BsuRI GGCC 2 cut(s) 55, 294
BtrI CACGTC 1 cut(s) 459
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 95
CfoI GCGC 1 cut(s) 77
Cfr13I GGNCC 1 cut(s) 54
CviJI RGCY 8 cut(s) 55, 132, 179, 294, 362, 378, 386, 467
CviKI_1 RGCY 8 cut(s) 55, 132, 179, 294, 362, 378, 386, 467
DdeI CTNAG 1 cut(s) 185
DpnI GATC 2 cut(s) 212, 351
DpnII GATC 2 cut(s) 210, 349
Eam1104I CTCTTC 3 cut(s) 120, 201, 387
EarI CTCTTC 3 cut(s) 120, 201, 387
Eco130I CCWWGG 1 cut(s) 387
Eco57I CTGAAG 3 cut(s) 110, 159, 225
EcoRII CCWGG 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
Esp3I CGTCTC 1 cut(s) 465
FaiI YATR 3 cut(s) 83, 192, 224
FaqI GGGAC 1 cut(s) 51
FauI CCCGC 1 cut(s) 161
Fnu4HI GCNGC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 44
GlaI GCGC 1 cut(s) 76
GluI GCNGC 1 cut(s) 44
GsuI CTGGAG 2 cut(s) 297, 458
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 2 cut(s) 55, 294
HhaI GCGC 1 cut(s) 77
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HindIII AAGCTT 1 cut(s) 465
HinfI GANTC 2 cut(s) 4, 200
HphI GGTGA 2 cut(s) 159, 262
Hpy166II GTNNAC 2 cut(s) 328, 404
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 2 cut(s) 70, 276
Hpy8I GTNNAC 2 cut(s) 328, 404
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 2 cut(s) 63, 319
HpyCH4IV ACGT 2 cut(s) 430, 458
HpyCH4V TGCA 3 cut(s) 226, 323, 355
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 368
HpyF3I CTNAG 1 cut(s) 185
HpySE526I ACGT 2 cut(s) 430, 458
HspAI GCGC 1 cut(s) 75
Kzo9I GATC 2 cut(s) 210, 349
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 2 cut(s) 72, 161
LpnPI CCDG 9 cut(s) 55, 70, 113, 172, 261, 276, 297, 422, 449
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 2 cut(s) 281, 358
MaeII ACGT 2 cut(s) 430, 458
MaeIII GTNAC 1 cut(s) 431
MalI GATC 2 cut(s) 212, 351
MbiI CCGCTC 1 cut(s) 156
MboI GATC 2 cut(s) 210, 349
MboII GAAGA 5 cut(s) 107, 141, 218, 404, 421
MflI RGATCY 2 cut(s) 210, 349
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 6 cut(s) 28, 45, 113, 175, 246, 341
MslI CAYNNNNRTG 2 cut(s) 83, 86
MspR9I CCNGG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 355
MvaI CCWGG 1 cut(s) 437
MwoI GCNNNNNNNGC 2 cut(s) 52, 368
NdeII GATC 2 cut(s) 210, 349
NmuCI GTSAC 1 cut(s) 431
PciSI GCTCTTC 1 cut(s) 120
PctI GAATGC 1 cut(s) 355
PfeI GAWTC 2 cut(s) 4, 200
PkrI GCNGC 1 cut(s) 45
Ppu21I YACGTR 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspPI GGNCC 1 cut(s) 54
PsuI RGATCY 2 cut(s) 210, 349
RseI CAYNNNNRTG 2 cut(s) 83, 86
SapI GCTCTTC 1 cut(s) 120
SatI GCNGC 1 cut(s) 44
Sau3AI GATC 2 cut(s) 210, 349
Sau96I GGNCC 1 cut(s) 54
ScrFI CCNGG 1 cut(s) 437
SfaNI GCATC 2 cut(s) 281, 358
SmiMI CAYNNNNRTG 2 cut(s) 83, 86
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
SsiI CCGC 1 cut(s) 154
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 2 cut(s) 63, 319
TaiI ACGT 2 cut(s) 433, 461
TfiI GAWTC 2 cut(s) 4, 200
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 1 cut(s) 431
TseI GCWGC 1 cut(s) 43
Tsp45I GTSAC 1 cut(s) 431
TspDTI ATGAA 2 cut(s) 17, 257
TspGWI ACGGA 2 cut(s) 203, 421
TspRI CASTG 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.