FvH4_4g05200

Small acidic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014128
Physical Location & Seq
Forward (+)
4464268 .. 4467420
3153 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05200.t1

Sequence Viewer

Length: 318 bp
ATGTCGCAATTCACGAGACAAAAAGCGAGCACTCCAGCGGAAATCCAATACGCTCGACGCTCAGCGGCGATAGCTTCAGTGAGACAGAGACGTTCTTCTTCTTCAGCTGCGACGACGACGACGATGAGGCCCACCGACTTCTTCGGCGACATGGACGACCAGGGCTCCACCATGGCCATGGACGTCGACGACCTCGACCCCCTCGAGTACCTCGGCGGCGACGGCGTCATCGGTGACAACAAGCTCACCGACCTCGACTTCTTCAACTCCTTCGAGGACGACTTCGACGAGTCCGACATGACCGCCCCCGCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

106

Amino Acids

11.54

Weight (kDa)

4.06

Isoelectric Point (pI)

58.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014128)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24280 AT4G13520 AT4G13520
fragaria_vesca FvH4_4g05200 FvH4_4g05200
prunus_persica Prupe.1G052700_v2.0.a1
pyrus_communis pycom16g19280
rosa_chinensis RchiOBHm_Chr4g0396111
rosa_laevigata RLG00000009571
rosa_multiflora Rmu_co8348141.1_g000001 Rmu_sc0007010.1_g000012 Rmu_sc0009634.1_g000009
rosa_roxburghii Rroxscaffold_5G00340910
rosa_rugosa Rorug03G0352300
rosa_samantha Rh4BG064300 Rh4CG070900
rosa_wichuraiana Rw4G005340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 186
AciI CCGC 5 cut(s) 38, 65, 216, 303, 309
AcoI YGGCCR 1 cut(s) 174
AcuI CTGAAG 2 cut(s) 60, 87
AcyI GRCGYC 2 cut(s) 183, 225
AfaI GTAC 1 cut(s) 209
AgsI TTSAA 1 cut(s) 265
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 3 cut(s) 74, 107, 244
AluI AGCT 3 cut(s) 74, 107, 244
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 3 cut(s) 10, 76, 82
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 2 cut(s) 128, 174
ApeKI GCWGC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 129
AsuHPI GGTGA 2 cut(s) 238, 245
AvaI CYCGRG 1 cut(s) 203
BalI TGGCCA 1 cut(s) 176
BanII GRGCYC 1 cut(s) 167
BauI CACGAG 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 94
BceAI ACGGC 1 cut(s) 238
BciT130I CCWGG 1 cut(s) 161
BcoDI GTCTC 3 cut(s) 10, 76, 82
BfaI CTAG 1 cut(s) 316
BisI GCNGC 3 cut(s) 66, 108, 217
BlpI GCTNAGC 1 cut(s) 61
BlsI GCNGC 3 cut(s) 67, 109, 218
Bme1390I CCNGG 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 129
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 1 cut(s) 161
BpmI CTGGAG 1 cut(s) 18
Bpu1102I GCTNAGC 1 cut(s) 61
BsaHI GRCGYC 2 cut(s) 183, 225
BsaJI CCNNGG 4 cut(s) 160, 171, 177, 211
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 4 cut(s) 160, 171, 177, 211
BseMII CTCAG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 94
BshFI GGCC 2 cut(s) 130, 176
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 203
BsmAI GTCTC 3 cut(s) 10, 76, 82
BsmBI CGTCTC 1 cut(s) 82
BsnI GGCC 2 cut(s) 130, 176
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 32, 167
Bsp1720I GCTNAGC 1 cut(s) 61
Bsp19I CCATGG 2 cut(s) 171, 177
BspACI CCGC 5 cut(s) 38, 65, 216, 303, 309
BspANI GGCC 2 cut(s) 130, 176
BspCNI CTCAG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 166
BssECI CCNNGG 4 cut(s) 160, 171, 177, 211
BssNI GRCGYC 2 cut(s) 183, 225
BssSI CACGAG 1 cut(s) 13
BssT1I CCWWGG 2 cut(s) 171, 177
Bst2BI CACGAG 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 161
BstACI GRCGYC 2 cut(s) 183, 225
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 1 cut(s) 61
BstDSI CCRYGG 2 cut(s) 171, 177
BstMAI GTCTC 3 cut(s) 10, 76, 82
BstMWI GCNNNNNNNGC 2 cut(s) 71, 222
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 1 cut(s) 159
BstV1I GCAGC 1 cut(s) 94
BstXI CCANNNNNNTGG 1 cut(s) 178
BsuRI GGCC 2 cut(s) 130, 176
BtgI CCRYGG 2 cut(s) 171, 177
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 28
Cfr13I GGNCC 1 cut(s) 129
CseI GACGC 2 cut(s) 66, 214
Csp6I GTAC 1 cut(s) 208
CviAII CATG 4 cut(s) 151, 172, 178, 298
CviJI RGCY 6 cut(s) 74, 107, 130, 165, 176, 244
CviKI_1 RGCY 6 cut(s) 74, 107, 130, 165, 176, 244
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 174
Eco130I CCWWGG 2 cut(s) 171, 177
Eco24I GRGCYC 1 cut(s) 167
Eco57I CTGAAG 2 cut(s) 60, 87
Eco88I CYCGRG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 159
EcoT14I CCWWGG 2 cut(s) 171, 177
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 2 cut(s) 171, 177
Esp3I CGTCTC 1 cut(s) 82
FaeI CATG 4 cut(s) 154, 175, 181, 301
FaiI YATR 4 cut(s) 152, 173, 179, 299
FatI CATG 4 cut(s) 150, 171, 177, 297
FauI CCCGC 1 cut(s) 316
FblI GTMKAC 1 cut(s) 186
Fnu4HI GCNGC 3 cut(s) 66, 108, 217
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 3 cut(s) 66, 108, 217
FspBI CTAG 1 cut(s) 316
GluI GCNGC 3 cut(s) 66, 108, 217
GsuI CTGGAG 1 cut(s) 18
HaeIII GGCC 2 cut(s) 130, 176
HgaI GACGC 2 cut(s) 66, 214
Hin1I GRCGYC 2 cut(s) 183, 225
Hin1II CATG 4 cut(s) 154, 175, 181, 301
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 1 cut(s) 290
HphI GGTGA 2 cut(s) 238, 245
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 1 cut(s) 295
Hpy188III TCNNGA 1 cut(s) 13
Hpy8I GTNNAC 1 cut(s) 187
Hpy99I CGWCG 9 cut(s) 60, 115, 118, 121, 124, 188, 191, 224, 290
HpyAV CCTTC 1 cut(s) 280
HpyCH4IV ACGT 2 cut(s) 91, 183
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 222
HpyF3I CTNAG 1 cut(s) 61
HpySE526I ACGT 2 cut(s) 91, 183
Hsp92I GRCGYC 2 cut(s) 183, 225
Hsp92II CATG 4 cut(s) 154, 175, 181, 301
LmnI GCTCC 1 cut(s) 170
LpnPI CCDG 3 cut(s) 48, 146, 173
Lsp1109I GCAGC 1 cut(s) 94
MaeI CTAG 1 cut(s) 316
MaeII ACGT 2 cut(s) 91, 183
MaeIII GTNAC 1 cut(s) 233
MboII GAAGA 5 cut(s) 87, 90, 93, 133, 253
MhlI GDGCHC 2 cut(s) 32, 167
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 1 cut(s) 8
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 318
MnlI CCTC 6 cut(s) 120, 203, 212, 221, 263, 268
Mox20I TGGCCA 1 cut(s) 176
MscI TGGCCA 1 cut(s) 176
MslI CAYNNNNRTG 1 cut(s) 176
Msp20I TGGCCA 1 cut(s) 176
MspA1I CMGCKG 3 cut(s) 38, 65, 107
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 2 cut(s) 71, 222
NcoI CCATGG 2 cut(s) 171, 177
NlaIII CATG 4 cut(s) 154, 175, 181, 301
NlaIV GGNNCC 1 cut(s) 166
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 1 cut(s) 233
PaeR7I CTCGAG 1 cut(s) 203
PcsI WCGNNNNNNNCGW 6 cut(s) 11, 119, 192, 201, 285, 291
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 3 cut(s) 67, 109, 218
PleI GAGTC 1 cut(s) 298
PpsI GAGTC 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 1 cut(s) 129
PspXI VCTCGAGB 1 cut(s) 203
PsyI GACNNNGTC 1 cut(s) 224
PvuII CAGCTG 1 cut(s) 107
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 176
SalI GTCGAC 1 cut(s) 185
SatI GCNGC 3 cut(s) 66, 108, 217
Sau96I GGNCC 1 cut(s) 129
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 1 cut(s) 161
SduI GDGCHC 2 cut(s) 32, 167
SetI ASST 8 cut(s) 76, 94, 109, 186, 195, 213, 246, 255
Sfr274I CTCGAG 1 cut(s) 203
SgrDI CGTCGACG 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 1 cut(s) 8
SsiI CCGC 5 cut(s) 38, 65, 216, 303, 309
SspMI CTAG 1 cut(s) 316
StyD4I CCNGG 1 cut(s) 159
StyI CCWWGG 2 cut(s) 171, 177
TaiI ACGT 2 cut(s) 94, 186
TaqI TCGA 7 cut(s) 55, 186, 195, 204, 255, 273, 285
TasI AATT 1 cut(s) 8
TauI GCSGC 2 cut(s) 68, 219
TscAI CASTG 1 cut(s) 84
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 233
TspRI CASTG 1 cut(s) 84
Tth111I GACNNNGTC 1 cut(s) 224
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 1 cut(s) 316
ZraI GACGTC 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.