FvH4_4g07191

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017643
Physical Location & Seq
Reverse (-)
6624486 .. 6625448
963 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07191.t1

Sequence Viewer

Length: 963 bp
ATGGTGCAGGTAGGTGTTGGAACTCCAGCCACAGGAGTGTTCTTGCTTCTAGACACCGGCGGTGGCCTAATCTGGACGCAGTGCCTACCTTGCTTAAACTGCTACCCCCAAAGAACTCCCATGTTCGACTCGAGTAAATCCAGCACGTATCAAAAGCTTCCTTGCAGCCACCCTTTTTGTCAGGGAGACCATCCCCTCTACCAATGTGTCAGTAATGAATGTGTCTACAATATTAACTACGGTGGAGGGGCATCAACTGCAGGCATCGCTTCCATAGAAACATTCACATTCCCCGTTGACACCGCCACAACTGTAAGACTTGGTCCAGTTGTTTTCGGCTGCTCAAAGTATACCAAAAACGTTCAATTTGCCAAAGGTGGTGCCATTTCAGGGATCTTGGGATTGAGCTTGTCTCCGGATTCGTTGGCTTCACAATTGGGTGCAGTGACACAGAAACGTTTCTCATATTGCTTAGTACCGTTCACTCAAGCACAGCAGGCACCAAGTCTTGTAAGGTTTGGGGATGATATTCCCCACCCATCCTCAAATATTCAGATAACTCCTTTTGTACCTACACCCGGATCTCATTACTTTCAGTTAAATTTGTTGGATATAAGTGTCGGATTTAACAAGCTAGGGTTACCCCCGGGTACCTTTGCCGTGGCGCCAGACGGTTCAGGCGGCTGCATCATCGACTCCGGTGCGCTGATTCCTCAGATCAATCAAAATGTTGTAGGAGGAATCAATGCCTACAGGAAGGTGATGTTTGCATTTCAAAGCTTCTATAATTCTTTCCAGCAGCTTACCAGGATCGGTAAAGTGCCTCAAGGGTTTGCATTGTGTTATAAGTATCCTCCAGATTTCAATCAATTCACCACTATGACATATCATTTTGAGGATGCGGATTATACTGTGGATTCCAAGTATGTGAATTTTTATGACGAGCAGGGAGGCTACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

321

Amino Acids

34.66

Weight (kDa)

6.17

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 2 - 175 5.4e-42 Xylanase inhibitor N-terminal
TAXi_C PF14541 200 - 307 1.4e-06 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017643)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07191
malus_domestica MD11G1223600.v1.1
prunus_persica Prupe.4G207600_v2.0.a1 Prupe.4G207700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399441
rosa_laevigata RLG00000009268
rosa_roxburghii Rroxscaffold_5G00344340
rosa_rugosa Rorug04G0016900
rosa_samantha Rh4BG092000 Rh4CG102900 Rh4DG086900
rosa_wichuraiana Rw4G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 846
Acc65I GGTACC 1 cut(s) 650
AccB1I GGYRCC 4 cut(s) 380, 499, 650, 664
AccI GTMKAC 2 cut(s) 225, 350
AccIII TCCGGA 1 cut(s) 415
AciI CCGC 4 cut(s) 60, 303, 681, 902
AclI AACGTT 2 cut(s) 360, 457
AclWI GGATC 3 cut(s) 401, 589, 818
AcsI RAATTY 2 cut(s) 601, 931
AcyI GRCGYC 1 cut(s) 665
AfaI GTAC 3 cut(s) 477, 570, 652
AfiI CCNNNNNNNGG 3 cut(s) 32, 390, 578
AgsI TTSAA 3 cut(s) 365, 776, 865
AjnI CCWGG 1 cut(s) 806
AleI CACNNNNGTG 1 cut(s) 35
AluBI AGCT 5 cut(s) 157, 408, 634, 780, 802
AluI AGCT 5 cut(s) 157, 408, 634, 780, 802
Alw26I GTCTC 2 cut(s) 180, 417
AlwI GGATC 3 cut(s) 401, 589, 818
Ama87I CYCGRG 2 cut(s) 130, 646
Aor13HI TCCGGA 1 cut(s) 415
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 4 cut(s) 165, 339, 684, 799
ApoI RAATTY 2 cut(s) 601, 931
Asp700I GAANNNNTTC 1 cut(s) 458
Asp718I GGTACC 1 cut(s) 650
AspLEI GCGC 2 cut(s) 667, 706
AspS9I GGNCC 1 cut(s) 323
AsuC2I CCSGG 3 cut(s) 579, 647, 648
AsuHPI GGTGA 2 cut(s) 772, 865
AvaI CYCGRG 2 cut(s) 130, 646
AvaII GGWCC 1 cut(s) 323
BanI GGYRCC 4 cut(s) 380, 499, 650, 664
BbvI GCAGC 4 cut(s) 177, 326, 671, 811
BccI CCATC 2 cut(s) 198, 547
BceAI ACGGC 1 cut(s) 644
BcgI CGANNNNNNTGC 2 cut(s) 683, 717
BciT130I CCWGG 1 cut(s) 808
BciVI GTATCC 1 cut(s) 861
BcnI CCSGG 3 cut(s) 579, 647, 648
BcoDI GTCTC 2 cut(s) 180, 417
BfaI CTAG 2 cut(s) 50, 635
BfmI CTRYAG 2 cut(s) 258, 751
BfoI RGCGCY 1 cut(s) 668
BfuI GTATCC 1 cut(s) 861
BisI GCNGC 5 cut(s) 166, 340, 682, 685, 800
BlsI GCNGC 5 cut(s) 167, 341, 683, 686, 801
Bme1390I CCNGG 4 cut(s) 579, 647, 648, 808
Bme18I GGWCC 1 cut(s) 323
BmeT110I CYCGRG 2 cut(s) 130, 646
BmgT120I GGNCC 1 cut(s) 323
BmiI GGNNCC 4 cut(s) 382, 501, 652, 666
BmrFI CCNGG 4 cut(s) 579, 647, 648, 808
BmsI GCATC 4 cut(s) 260, 273, 696, 889
BplI GAGNNNNNCTC 2 cut(s) 397, 429
BpmI CTGGAG 2 cut(s) 9, 840
BpuEI CTTGAG 2 cut(s) 471, 810
BpuMI CCSGG 3 cut(s) 579, 647, 648
BsaAI YACGTR 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 864
BsaHI GRCGYC 1 cut(s) 665
BsaI GGTCTC 1 cut(s) 180
BsaJI CCNNGG 3 cut(s) 645, 646, 660
BsaWI WCCGGW 2 cut(s) 415, 698
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 390, 578
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 326
Bse8I GATNNNNATC 1 cut(s) 864
BseAI TCCGGA 1 cut(s) 415
BseBI CCWGG 1 cut(s) 808
BseDI CCNNGG 3 cut(s) 645, 646, 660
BseGI GGATG 4 cut(s) 190, 529, 539, 904
BseJI GATNNNNATC 1 cut(s) 864
BseLI CCNNNNNNNGG 3 cut(s) 32, 390, 578
BseMII CTCAG 1 cut(s) 728
BseNI ACTGG 1 cut(s) 326
BseXI GCAGC 4 cut(s) 177, 326, 671, 811
BsgI GTGCAG 2 cut(s) 26, 462
BshFI GGCC 1 cut(s) 66
BshNI GGYRCC 4 cut(s) 380, 499, 650, 664
BsiHKCI CYCGRG 2 cut(s) 130, 646
BsiSI CCGG 5 cut(s) 57, 416, 579, 647, 699
BslI CCNNNNNNNGG 3 cut(s) 32, 390, 578
BsmAI GTCTC 2 cut(s) 180, 417
BsnI GGCC 1 cut(s) 66
Bso31I GGTCTC 1 cut(s) 180
BsoBI CYCGRG 2 cut(s) 130, 646
Bsp13I TCCGGA 1 cut(s) 415
Bsp143I GATC 4 cut(s) 393, 581, 717, 810
BspACI CCGC 4 cut(s) 60, 303, 681, 902
BspANI GGCC 1 cut(s) 66
BspCNI CTCAG 1 cut(s) 727
BspEI TCCGGA 1 cut(s) 415
BspLI GGNNCC 4 cut(s) 382, 501, 652, 666
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 3 cut(s) 401, 589, 818
BspT107I GGYRCC 4 cut(s) 380, 499, 650, 664
BspTNI GGTCTC 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 326
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 3 cut(s) 645, 646, 660
BssMI GATC 4 cut(s) 393, 581, 717, 810
BssNAI GTATAC 1 cut(s) 351
BssNI GRCGYC 1 cut(s) 665
Bst1107I GTATAC 1 cut(s) 351
Bst2UI CCWGG 1 cut(s) 808
Bst4CI ACNGT 5 cut(s) 242, 313, 480, 674, 913
BstACI GRCGYC 1 cut(s) 665
BstAPI GCANNNNNTGC 1 cut(s) 257
BstBAI YACGTR 1 cut(s) 147
BstC8I GCNNGC 2 cut(s) 262, 498
BstDEI CTNAG 2 cut(s) 472, 714
BstDSI CCRYGG 1 cut(s) 660
BstEII GGTNACC 1 cut(s) 639
BstF5I GGATG 4 cut(s) 190, 529, 539, 904
BstH2I RGCGCY 1 cut(s) 668
BstHHI GCGC 2 cut(s) 667, 706
BstKTI GATC 4 cut(s) 396, 584, 720, 813
BstMAI GTCTC 2 cut(s) 180, 417
BstMBI GATC 4 cut(s) 393, 581, 717, 810
BstMWI GCNNNNNNNGC 5 cut(s) 90, 99, 257, 266, 497
BstNI CCWGG 1 cut(s) 808
BstPI GGTNACC 1 cut(s) 639
BstSCI CCNGG 4 cut(s) 577, 645, 646, 806
BstSFI CTRYAG 2 cut(s) 258, 751
BstV1I GCAGC 4 cut(s) 177, 326, 671, 811
BstX2I RGATCY 2 cut(s) 393, 581
BstYI RGATCY 2 cut(s) 393, 581
BstZ17I GTATAC 1 cut(s) 351
BsuI GTATCC 1 cut(s) 861
BsuRI GGCC 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 660
BtgZI GCGATG 1 cut(s) 250
BtsCI GGATG 4 cut(s) 190, 529, 539, 904
BtsI GCAGTG 2 cut(s) 86, 450
BtsIMutI CAGTG 2 cut(s) 86, 450
Cac8I GCNNGC 2 cut(s) 262, 498
CfoI GCGC 2 cut(s) 667, 706
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 1 cut(s) 323
Cfr9I CCCGGG 1 cut(s) 646
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 476, 569, 651
CspCI CAANNNNNGTGG 2 cut(s) 158, 193
CviAII CATG 1 cut(s) 121
CviQI GTAC 3 cut(s) 476, 569, 651
DdeI CTNAG 2 cut(s) 472, 714
DinI GGCGCC 1 cut(s) 666
DpnI GATC 4 cut(s) 395, 583, 719, 812
DpnII GATC 4 cut(s) 393, 581, 717, 810
Eco31I GGTCTC 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 323
Eco88I CYCGRG 2 cut(s) 130, 646
Eco91I GGTNACC 1 cut(s) 639
EcoO65I GGTNACC 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 806
EgeI GGCGCC 1 cut(s) 666
EheI GGCGCC 1 cut(s) 666
FaeI CATG 1 cut(s) 124
FatI CATG 1 cut(s) 120
FblI GTMKAC 2 cut(s) 225, 350
Fnu4HI GCNGC 5 cut(s) 166, 340, 682, 685, 800
FokI GGATG 4 cut(s) 177, 526, 536, 911
Fsp4HI GCNGC 5 cut(s) 166, 340, 682, 685, 800
FspBI CTAG 2 cut(s) 50, 635
GlaI GCGC 2 cut(s) 666, 705
GluI GCNGC 5 cut(s) 166, 340, 682, 685, 800
GsuI CTGGAG 2 cut(s) 9, 840
HaeII RGCGCY 1 cut(s) 668
HaeIII GGCC 1 cut(s) 66
HapII CCGG 5 cut(s) 57, 416, 579, 647, 699
HgaI GACGC 1 cut(s) 85
HhaI GCGC 2 cut(s) 667, 706
Hin1I GRCGYC 1 cut(s) 665
Hin1II CATG 1 cut(s) 124
Hin6I GCGC 2 cut(s) 665, 704
HinP1I GCGC 2 cut(s) 665, 704
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HindIII AAGCTT 2 cut(s) 155, 778
HinfI GANTC 6 cut(s) 128, 419, 695, 709, 741, 917
HpaII CCGG 5 cut(s) 57, 416, 579, 647, 699
HphI GGTGA 2 cut(s) 772, 865
Hpy166II GTNNAC 4 cut(s) 226, 298, 351, 483
Hpy188I TCNGA 3 cut(s) 555, 623, 717
Hpy188III TCNNGA 4 cut(s) 50, 73, 416, 857
Hpy8I GTNNAC 4 cut(s) 226, 298, 351, 483
HpyAV CCTTC 1 cut(s) 751
HpyCH4III ACNGT 5 cut(s) 242, 313, 480, 674, 913
HpyCH4IV ACGT 3 cut(s) 146, 360, 457
HpyCH4V TGCA 7 cut(s) 7, 165, 260, 443, 687, 770, 836
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 99, 257, 266, 497
HpyF3I CTNAG 2 cut(s) 472, 714
HpySE526I ACGT 3 cut(s) 146, 360, 457
Hsp92I GRCGYC 1 cut(s) 665
Hsp92II CATG 1 cut(s) 124
HspAI GCGC 2 cut(s) 665, 704
KasI GGCGCC 1 cut(s) 664
Kpn2I TCCGGA 1 cut(s) 415
KpnI GGTACC 1 cut(s) 654
Kzo9I GATC 4 cut(s) 393, 581, 717, 810
Lsp1109I GCAGC 4 cut(s) 177, 326, 671, 811
LweI GCATC 4 cut(s) 260, 273, 696, 889
MaeI CTAG 2 cut(s) 50, 635
MaeII ACGT 3 cut(s) 146, 360, 457
MaeIII GTNAC 2 cut(s) 445, 639
MalI GATC 4 cut(s) 395, 583, 719, 812
MboI GATC 4 cut(s) 393, 581, 717, 810
MfeI CAATTG 1 cut(s) 434
MflI RGATCY 2 cut(s) 393, 581
MluCI AATT 6 cut(s) 365, 434, 601, 787, 869, 931
Mly113I GGCGCC 1 cut(s) 665
MlyI GAGTC 2 cut(s) 122, 689
MmeI TCCRAC 2 cut(s) 588, 601
MnlI CCTC 9 cut(s) 206, 239, 553, 723, 731, 834, 864, 889, 944
MroI TCCGGA 1 cut(s) 415
MroXI GAANNNNTTC 1 cut(s) 458
MseI TTAA 4 cut(s) 95, 234, 599, 627
MslI CAYNNNNRTG 2 cut(s) 35, 878
MspI CCGG 5 cut(s) 57, 416, 579, 647, 699
MspR9I CCNGG 4 cut(s) 579, 647, 648, 808
MunI CAATTG 1 cut(s) 434
MvaI CCWGG 1 cut(s) 808
MwoI GCNNNNNNNGC 5 cut(s) 90, 99, 257, 266, 497
NarI GGCGCC 1 cut(s) 665
NciI CCSGG 3 cut(s) 579, 647, 648
NdeII GATC 4 cut(s) 393, 581, 717, 810
NlaIII CATG 1 cut(s) 124
NlaIV GGNNCC 4 cut(s) 382, 501, 652, 666
NmuCI GTSAC 1 cut(s) 445
OliI CACNNNNGTG 1 cut(s) 35
PaeR7I CTCGAG 1 cut(s) 130
PdmI GAANNNNTTC 1 cut(s) 458
PfeI GAWTC 4 cut(s) 419, 709, 741, 917
PflFI GACNNNGTC 1 cut(s) 321
PkrI GCNGC 5 cut(s) 167, 341, 683, 686, 801
PleI GAGTC 2 cut(s) 122, 689
PluTI GGCGCC 1 cut(s) 668
PpsI GAGTC 2 cut(s) 122, 689
Ppu21I YACGTR 1 cut(s) 147
PsiI TTATAA 1 cut(s) 846
Psp1406I AACGTT 2 cut(s) 360, 457
Psp6I CCWGG 1 cut(s) 806
PspEI GGTNACC 1 cut(s) 639
PspGI CCWGG 1 cut(s) 806
PspN4I GGNNCC 4 cut(s) 382, 501, 652, 666
PspPI GGNCC 1 cut(s) 323
PspXI VCTCGAGB 1 cut(s) 130
PstI CTGCAG 1 cut(s) 262
PsuI RGATCY 2 cut(s) 393, 581
PsyI GACNNNGTC 1 cut(s) 321
RsaI GTAC 3 cut(s) 477, 570, 652
RsaNI GTAC 3 cut(s) 476, 569, 651
RseI CAYNNNNRTG 2 cut(s) 35, 878
SaqAI TTAA 4 cut(s) 95, 234, 599, 627
SatI GCNGC 5 cut(s) 166, 340, 682, 685, 800
Sau3AI GATC 4 cut(s) 393, 581, 717, 810
Sau96I GGNCC 1 cut(s) 323
SchI GAGTC 2 cut(s) 122, 689
ScrFI CCNGG 4 cut(s) 579, 647, 648, 808
SfaNI GCATC 4 cut(s) 260, 273, 696, 889
SfcI CTRYAG 2 cut(s) 258, 751
SfoI GGCGCC 1 cut(s) 666
Sfr274I CTCGAG 1 cut(s) 130
SgrAI CRCCGGYG 1 cut(s) 56
SinI GGWCC 1 cut(s) 323
SlaI CTCGAG 1 cut(s) 130
SmaI CCCGGG 1 cut(s) 648
SmiMI CAYNNNNRTG 2 cut(s) 35, 878
SmlI CTYRAG 3 cut(s) 130, 486, 825
SmoI CTYRAG 3 cut(s) 130, 486, 825
Sse9I AATT 6 cut(s) 365, 434, 601, 787, 869, 931
SsiI CCGC 4 cut(s) 60, 303, 681, 902
SspDI GGCGCC 1 cut(s) 664
SspI AATATT 2 cut(s) 232, 550
SspMI CTAG 2 cut(s) 50, 635
StyD4I CCNGG 4 cut(s) 577, 645, 646, 806
TaaI ACNGT 5 cut(s) 242, 313, 480, 674, 913
TaiI ACGT 3 cut(s) 149, 363, 460
TaqI TCGA 3 cut(s) 126, 131, 693
TasI AATT 6 cut(s) 365, 434, 601, 787, 869, 931
TauI GCSGC 1 cut(s) 684
TfiI GAWTC 4 cut(s) 419, 709, 741, 917
Tru1I TTAA 4 cut(s) 95, 234, 599, 627
Tru9I TTAA 4 cut(s) 95, 234, 599, 627
TscAI CASTG 2 cut(s) 86, 450
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 4 cut(s) 165, 339, 684, 799
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 1 cut(s) 231
TspMI CCCGGG 1 cut(s) 646
TspRI CASTG 2 cut(s) 86, 450
Tth111I GACNNNGTC 1 cut(s) 321
VpaK11BI GGWCC 1 cut(s) 323
XapI RAATTY 2 cut(s) 601, 931
XbaI TCTAGA 1 cut(s) 49
XhoI CTCGAG 1 cut(s) 130
XmaI CCCGGG 1 cut(s) 646
XmiI GTMKAC 2 cut(s) 225, 350
XmnI GAANNNNTTC 1 cut(s) 458
XspI CTAG 2 cut(s) 50, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.