Rh4DG086900

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017643
Physical Location & Seq
Reverse (-)
13294463 .. 13295839
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG086900.1

Sequence Viewer

Length: 1377 bp
ATGGCAGCAGCTTCCCAACCCGTTTTAATCTTTTTCCTCATTGCAGTCTCTGCATTCCATCAAACACTTTCAAAACCTAATGGTTTCAGCCTCAAGCTCATCCCCAGAGACTCCCCCGAGTCTCCTCTGTACCCAGGAAACCTCACTACACTTGAACGAATCGAAAGATTGATCGAGTTCTCCCGAGCCAGGGCTCGCTATGTGGAACTAACTTCGTCTCCAAATGCAACCATCTTGTACCCCGAAAACATACGTCTCACTTTGCTTCGTGATAACTTCTTCTACACGGTGCAGGTAGGTGTTGGAACTCCAGCCACAGGAGTGTTCCTGCTTCTAGACACTGGCGGTGGCCTAATCTGGACGCAGTGCCTACCTTGCTTAAATTGCTATCCGCAAAGAACTCCCATGTTTGATTCGAGTAAATCCAGCACTTATCAAAAGCTTCCTTGCAGCCACCGTTTTTGTCAAGGCGACCGTCCCCTCTACAAATGTGTCAATGATGAGTGTGTCTACAACATTAACTACGGTGGAGGGGCATCGACTGCAGGCATCGCTTCCATGGAAACATTCACATTTCCAGTCGACACCGCCACTACTACTCGAATTGGTCAAATTGTTTTTGGTTGCTCAAAGTATAGCCGAAACGTTCAATTTGCCAAAGGTGGTGTCATTTCAGGGGTCATGGGATTGAGCTTGTCTCCGGATTCATTGGCTTCACAGCTGTCTGATGTGACACAGAAACGTTTCTCATATTGCTTGGTACCTTTCACTCAAGCACAGCAGGCACCAAGTCTGGTAAGGTTTGGGGATGATATTCCCCACCCATCCTCAAATATTCAGACAACTCCTTTTGTACCAACACCCGGATCCCATTACTTTCAATTAAATTTGTTGGACATAAGTGTCGGATTTCGCAAGCTAGGGTTTCCCCCAGGTACCTTTACGGCGAGGCCAGATGGTTCAGGCGGCTGCATCGTTGACTCCGGTGCACTGATACCTCAGATCGATCAAAATGCCCAAGGCGGACGCAACGCCTACAGGCAGGTGATGTCTGCGTTTCAGAGCTATTATAATTCTTTCCAGCAGCTTACGAGGATCGGTAAAGTGCCTCAAGGGTTTGCATTGTGTTACAAGTATCCCCAAGATTTTAATCAATTTACCAGCATGACATATCATTTTGAGGATGCGGATTATATTGTGGATCCGAAATATGTGAATTTTTATGATCAGCAGGGAGGCTACTTTTGTGTTGCGTTGTTGCCTGGAAATGGGAAGAGTATACTTGGAGCATGGCATCAGCAAAATATGAGGGTGACGTACGATGGCAACATTAACTCACTCCAATTCTCTGTTGAGACTTGTTCTTATGATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

458

Amino Acids

50.62

Weight (kDa)

7.88

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 95 - 270 2e-42 Xylanase inhibitor N-terminal
Asp PF00026 95 - 449 1.3e-10 Eukaryotic aspartyl protease
TAXi_C PF14541 295 - 450 8e-16 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017643)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07191
malus_domestica MD11G1223600.v1.1
prunus_persica Prupe.4G207600_v2.0.a1 Prupe.4G207700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399441
rosa_laevigata RLG00000009268
rosa_roxburghii Rroxscaffold_5G00344340
rosa_rugosa Rorug04G0016900
rosa_samantha Rh4BG092000 Rh4CG102900 Rh4DG086900
rosa_wichuraiana Rw4G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1071
AarI CACCTGC 1 cut(s) 1033
AasI GACNNNNNNGTC 1 cut(s) 902
Acc36I ACCTGC 2 cut(s) 283, 1033
Acc65I GGTACC 2 cut(s) 760, 935
AccB1I GGYRCC 3 cut(s) 760, 784, 935
AccI GTMKAC 3 cut(s) 510, 582, 1279
AccIII TCCGGA 1 cut(s) 700
AciI CCGC 6 cut(s) 345, 392, 588, 966, 1023, 1187
AclI AACGTT 2 cut(s) 645, 742
AclWI GGATC 5 cut(s) 861, 874, 1103, 1196, 1209
AcsI RAATTY 2 cut(s) 886, 1216
AfaI GTAC 6 cut(s) 131, 239, 762, 855, 937, 1319
AfiI CCNNNNNNNGG 5 cut(s) 189, 190, 317, 863, 1268
AgsI TTSAA 4 cut(s) 72, 155, 650, 881
AjnI CCWGG 4 cut(s) 133, 188, 931, 1261
AleI CACNNNNGTG 1 cut(s) 320
AluBI AGCT 8 cut(s) 11, 97, 442, 693, 721, 919, 1065, 1087
AluI AGCT 8 cut(s) 11, 97, 442, 693, 721, 919, 1065, 1087
Alw21I GWGCWC 1 cut(s) 991
Alw26I GTCTC 7 cut(s) 52, 102, 126, 222, 260, 702, 1349
Alw44I GTGCAC 1 cut(s) 987
AlwI GGATC 5 cut(s) 861, 874, 1103, 1196, 1209
AlwNI CAGNNNCTG 1 cut(s) 50
Ama87I CYCGRG 2 cut(s) 116, 183
Aor13HI TCCGGA 1 cut(s) 700
AoxI GGCC 2 cut(s) 349, 950
ApaLI GTGCAC 1 cut(s) 987
ApeKI GCWGC 5 cut(s) 5, 8, 450, 969, 1084
ApoI RAATTY 2 cut(s) 886, 1216
ArsI GACNNNNNNTTYG 2 cut(s) 651, 683
Asp700I GAANNNNTTC 1 cut(s) 743
Asp718I GGTACC 2 cut(s) 760, 935
AsuC2I CCSGG 1 cut(s) 864
AsuHPI GGTGA 2 cut(s) 1057, 1324
AvaI CYCGRG 2 cut(s) 116, 183
BaeGI GKGCMC 1 cut(s) 991
BamHI GGATCC 2 cut(s) 866, 1201
BanI GGYRCC 3 cut(s) 760, 784, 935
BanII GRGCYC 1 cut(s) 196
Bbv12I GWGCWC 1 cut(s) 991
BbvI GCAGC 5 cut(s) 17, 20, 462, 956, 1096
BccI CCATC 5 cut(s) 66, 239, 832, 950, 1316
BceAI ACGGC 1 cut(s) 960
BcgI CGANNNNNNTGC 2 cut(s) 995, 1029
BciT130I CCWGG 4 cut(s) 135, 190, 933, 1263
BciVI GTATCC 1 cut(s) 1146
BclI TGATCA 1 cut(s) 1225
BcnI CCSGG 1 cut(s) 864
BcoDI GTCTC 7 cut(s) 52, 102, 126, 222, 260, 702, 1349
BfaI CTAG 2 cut(s) 335, 920
BfmI CTRYAG 2 cut(s) 543, 1036
BfuAI ACCTGC 2 cut(s) 283, 1033
BfuI GTATCC 1 cut(s) 1146
BisI GCNGC 6 cut(s) 6, 9, 451, 967, 970, 1085
BlsI GCNGC 6 cut(s) 7, 10, 452, 968, 971, 1086
Bme1390I CCNGG 5 cut(s) 135, 190, 864, 933, 1263
BmeT110I CYCGRG 2 cut(s) 116, 183
BmiI GGNNCC 5 cut(s) 762, 786, 868, 937, 1203
BmrFI CCNGG 5 cut(s) 135, 190, 864, 933, 1263
BmsI GCATC 5 cut(s) 545, 558, 981, 1174, 1303
BplI GAGNNNNNCTC 2 cut(s) 682, 714
BpmI CTGGAG 1 cut(s) 294
BpuEI CTTGAG 3 cut(s) 77, 756, 1095
BpuMI CCSGG 1 cut(s) 864
Bsa29I ATCGAT 1 cut(s) 1005
BsaBI GATNNNNATC 1 cut(s) 1149
BsaJI CCNNGG 5 cut(s) 133, 189, 558, 931, 1018
BsaWI WCCGGW 2 cut(s) 700, 983
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 5 cut(s) 189, 190, 317, 863, 1268
Bse1I ACTGG 2 cut(s) 346, 578
Bse3DI GCAATG 1 cut(s) 39
Bse8I GATNNNNATC 1 cut(s) 1149
BseAI TCCGGA 1 cut(s) 700
BseBI CCWGG 4 cut(s) 135, 190, 933, 1263
BseCI ATCGAT 1 cut(s) 1005
BseDI CCNNGG 5 cut(s) 133, 189, 558, 931, 1018
BseGI GGATG 4 cut(s) 99, 814, 824, 1189
BseJI GATNNNNATC 1 cut(s) 1149
BseLI CCNNNNNNNGG 5 cut(s) 189, 190, 317, 863, 1268
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 1 cut(s) 1013
BseNI ACTGG 2 cut(s) 346, 578
BseRI GAGGAG 1 cut(s) 114
BseSI GKGCMC 1 cut(s) 991
BseXI GCAGC 5 cut(s) 17, 20, 462, 956, 1096
BsgI GTGCAG 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 475
BshFI GGCC 2 cut(s) 351, 952
BshNI GGYRCC 3 cut(s) 760, 784, 935
BshVI ATCGAT 1 cut(s) 1005
BsiEI CGRYCG 1 cut(s) 475
BsiHKAI GWGCWC 1 cut(s) 991
BsiHKCI CYCGRG 2 cut(s) 116, 183
BsiSI CCGG 3 cut(s) 701, 864, 984
BsiWI CGTACG 1 cut(s) 1317
BslFI GGGAC 1 cut(s) 462
BslI CCNNNNNNNGG 5 cut(s) 189, 190, 317, 863, 1268
BsmAI GTCTC 7 cut(s) 52, 102, 126, 222, 260, 702, 1349
BsmBI CGTCTC 2 cut(s) 222, 260
BsmFI GGGAC 1 cut(s) 462
BsmI GAATGC 1 cut(s) 53
BsnI GGCC 2 cut(s) 351, 952
BsoBI CYCGRG 2 cut(s) 116, 183
Bsp1286I GDGCHC 2 cut(s) 196, 991
Bsp13I TCCGGA 1 cut(s) 700
Bsp143I GATC 7 cut(s) 171, 866, 1002, 1006, 1095, 1201, 1225
Bsp19I CCATGG 1 cut(s) 558
BspACI CCGC 6 cut(s) 345, 392, 588, 966, 1023, 1187
BspANI GGCC 2 cut(s) 351, 952
BspCNI CTCAG 1 cut(s) 1012
BspDI ATCGAT 1 cut(s) 1005
BspEI TCCGGA 1 cut(s) 700
BspLI GGNNCC 5 cut(s) 762, 786, 868, 937, 1203
BspMAI CTGCAG 1 cut(s) 547
BspMI ACCTGC 2 cut(s) 283, 1033
BspPI GGATC 5 cut(s) 861, 874, 1103, 1196, 1209
BspT107I GGYRCC 3 cut(s) 760, 784, 935
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 2 cut(s) 346, 578
BssECI CCNNGG 5 cut(s) 133, 189, 558, 931, 1018
BssMI GATC 7 cut(s) 171, 866, 1002, 1006, 1095, 1201, 1225
BssNAI GTATAC 1 cut(s) 1280
BssT1I CCWWGG 2 cut(s) 558, 1018
Bst1107I GTATAC 1 cut(s) 1280
Bst2UI CCWGG 4 cut(s) 135, 190, 933, 1263
Bst4CI ACNGT 4 cut(s) 289, 458, 476, 527
Bst6I CTCTTC 1 cut(s) 1268
BstAPI GCANNNNNTGC 2 cut(s) 50, 542
BstC8I GCNNGC 4 cut(s) 196, 547, 783, 917
BstDEI CTNAG 1 cut(s) 999
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 4 cut(s) 99, 814, 824, 1189
BstKTI GATC 7 cut(s) 174, 869, 1005, 1009, 1098, 1204, 1228
BstMAI GTCTC 7 cut(s) 52, 102, 126, 222, 260, 702, 1349
BstMBI GATC 7 cut(s) 171, 866, 1002, 1006, 1095, 1201, 1225
BstMCI CGRYCG 1 cut(s) 475
BstMWI GCNNNNNNNGC 6 cut(s) 50, 375, 384, 542, 551, 782
BstNI CCWGG 4 cut(s) 135, 190, 933, 1263
BstSCI CCNGG 5 cut(s) 133, 188, 862, 931, 1261
BstSFI CTRYAG 2 cut(s) 543, 1036
BstSLI GKGCMC 1 cut(s) 991
BstV1I GCAGC 5 cut(s) 17, 20, 462, 956, 1096
BstX2I RGATCY 2 cut(s) 866, 1201
BstYI RGATCY 2 cut(s) 866, 1201
BstZ17I GTATAC 1 cut(s) 1280
Bsu15I ATCGAT 1 cut(s) 1005
BsuI GTATCC 1 cut(s) 1146
BsuRI GGCC 2 cut(s) 351, 952
BsuTUI ATCGAT 1 cut(s) 1005
BtgI CCRYGG 1 cut(s) 558
BtgZI GCGATG 1 cut(s) 535
BtsCI GGATG 4 cut(s) 99, 814, 824, 1189
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 3 cut(s) 339, 371, 989
BveI ACCTGC 2 cut(s) 283, 1033
Cac8I GCNNGC 4 cut(s) 196, 547, 783, 917
CaiI CAGNNNCTG 1 cut(s) 50
ClaI ATCGAT 1 cut(s) 1005
CseI GACGC 2 cut(s) 370, 1035
Csp6I GTAC 6 cut(s) 130, 238, 761, 854, 936, 1318
CspCI CAANNNNNGTGG 2 cut(s) 443, 478
CviAII CATG 5 cut(s) 406, 559, 682, 1165, 1290
CviQI GTAC 6 cut(s) 130, 238, 761, 854, 936, 1318
DdeI CTNAG 1 cut(s) 999
DpnI GATC 7 cut(s) 173, 868, 1004, 1008, 1097, 1203, 1227
DpnII GATC 7 cut(s) 171, 866, 1002, 1006, 1095, 1201, 1225
DrdI GACNNNNNNGTC 1 cut(s) 902
DseDI GACNNNNNNGTC 1 cut(s) 902
Eam1104I CTCTTC 1 cut(s) 1268
EarI CTCTTC 1 cut(s) 1268
EciI GGCGGA 1 cut(s) 1038
Eco130I CCWWGG 2 cut(s) 558, 1018
Eco24I GRGCYC 1 cut(s) 196
Eco88I CYCGRG 2 cut(s) 116, 183
EcoRII CCWGG 4 cut(s) 133, 188, 931, 1261
EcoT14I CCWWGG 2 cut(s) 558, 1018
EcoT38I GRGCYC 1 cut(s) 196
ErhI CCWWGG 2 cut(s) 558, 1018
Esp3I CGTCTC 2 cut(s) 222, 260
FaeI CATG 5 cut(s) 409, 562, 685, 1168, 1293
FaqI GGGAC 1 cut(s) 462
FatI CATG 5 cut(s) 405, 558, 681, 1164, 1289
FbaI TGATCA 1 cut(s) 1225
FblI GTMKAC 3 cut(s) 510, 582, 1279
Fnu4HI GCNGC 6 cut(s) 6, 9, 451, 967, 970, 1085
FokI GGATG 4 cut(s) 86, 811, 821, 1196
FriOI GRGCYC 1 cut(s) 196
Fsp4HI GCNGC 6 cut(s) 6, 9, 451, 967, 970, 1085
FspBI CTAG 2 cut(s) 335, 920
GluI GCNGC 6 cut(s) 6, 9, 451, 967, 970, 1085
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 2 cut(s) 351, 952
HapII CCGG 3 cut(s) 701, 864, 984
HgaI GACGC 2 cut(s) 370, 1035
Hin1II CATG 5 cut(s) 409, 562, 685, 1168, 1293
HincII GTYRAC 2 cut(s) 583, 979
HindII GTYRAC 2 cut(s) 583, 979
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 6 cut(s) 110, 119, 159, 413, 704, 980
HpaII CCGG 3 cut(s) 701, 864, 984
HphI GGTGA 2 cut(s) 1057, 1324
Hpy166II GTNNAC 5 cut(s) 511, 583, 979, 989, 1280
Hpy188I TCNGA 6 cut(s) 727, 840, 908, 1002, 1062, 1206
Hpy188III TCNNGA 5 cut(s) 183, 269, 335, 358, 701
Hpy8I GTNNAC 5 cut(s) 511, 583, 979, 989, 1280
HpyCH4III ACNGT 4 cut(s) 289, 458, 476, 527
HpyCH4IV ACGT 4 cut(s) 253, 645, 742, 1316
HpyCH4V TGCA 9 cut(s) 44, 53, 227, 292, 450, 545, 972, 989, 1121
HpyF10VI GCNNNNNNNGC 6 cut(s) 50, 375, 384, 542, 551, 782
HpyF3I CTNAG 1 cut(s) 999
HpySE526I ACGT 4 cut(s) 253, 645, 742, 1316
Hsp92II CATG 5 cut(s) 409, 562, 685, 1168, 1293
Kpn2I TCCGGA 1 cut(s) 700
KpnI GGTACC 2 cut(s) 764, 939
Ksp22I TGATCA 1 cut(s) 1225
Kzo9I GATC 7 cut(s) 171, 866, 1002, 1006, 1095, 1201, 1225
LmnI GCTCC 1 cut(s) 1286
Lsp1109I GCAGC 5 cut(s) 17, 20, 462, 956, 1096
LweI GCATC 5 cut(s) 545, 558, 981, 1174, 1303
MaeI CTAG 2 cut(s) 335, 920
MaeII ACGT 4 cut(s) 253, 645, 742, 1316
MaeIII GTNAC 3 cut(s) 730, 1127, 1312
MalI GATC 7 cut(s) 173, 868, 1004, 1008, 1097, 1203, 1227
MboI GATC 7 cut(s) 171, 866, 1002, 1006, 1095, 1201, 1225
MboII GAAGA 2 cut(s) 271, 1285
MflI RGATCY 2 cut(s) 866, 1201
MhlI GDGCHC 2 cut(s) 196, 991
MlyI GAGTC 3 cut(s) 104, 128, 974
MmeI TCCRAC 3 cut(s) 283, 873, 886
MroI TCCGGA 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 743
MseI TTAA 7 cut(s) 26, 380, 519, 884, 1149, 1332, 1375
MslI CAYNNNNRTG 1 cut(s) 320
MspA1I CMGCKG 1 cut(s) 721
MspI CCGG 3 cut(s) 701, 864, 984
MspR9I CCNGG 5 cut(s) 135, 190, 864, 933, 1263
Mva1269I GAATGC 1 cut(s) 53
MvaI CCWGG 4 cut(s) 135, 190, 933, 1263
MwoI GCNNNNNNNGC 6 cut(s) 50, 375, 384, 542, 551, 782
NciI CCSGG 1 cut(s) 864
NcoI CCATGG 1 cut(s) 558
NdeII GATC 7 cut(s) 171, 866, 1002, 1006, 1095, 1201, 1225
NlaIII CATG 5 cut(s) 409, 562, 685, 1168, 1293
NlaIV GGNNCC 5 cut(s) 762, 786, 868, 937, 1203
NmuCI GTSAC 2 cut(s) 730, 1312
OliI CACNNNNGTG 1 cut(s) 320
PaqCI CACCTGC 1 cut(s) 1033
PctI GAATGC 1 cut(s) 53
PdmI GAANNNNTTC 1 cut(s) 743
PfeI GAWTC 3 cut(s) 159, 413, 704
Pfl23II CGTACG 1 cut(s) 1317
PkrI GCNGC 6 cut(s) 7, 10, 452, 968, 971, 1086
PleI GAGTC 3 cut(s) 104, 127, 974
PpsI GAGTC 3 cut(s) 104, 127, 974
PsiI TTATAA 1 cut(s) 1071
Psp1406I AACGTT 2 cut(s) 645, 742
Psp6I CCWGG 4 cut(s) 133, 188, 931, 1261
PspGI CCWGG 4 cut(s) 133, 188, 931, 1261
PspLI CGTACG 1 cut(s) 1317
PspN4I GGNNCC 5 cut(s) 762, 786, 868, 937, 1203
PstI CTGCAG 1 cut(s) 547
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 2 cut(s) 866, 1201
PvuII CAGCTG 1 cut(s) 721
RsaI GTAC 6 cut(s) 131, 239, 762, 855, 937, 1319
RsaNI GTAC 6 cut(s) 130, 238, 761, 854, 936, 1318
RseI CAYNNNNRTG 1 cut(s) 320
SalI GTCGAC 1 cut(s) 581
SaqAI TTAA 7 cut(s) 26, 380, 519, 884, 1149, 1332, 1375
SatI GCNGC 6 cut(s) 6, 9, 451, 967, 970, 1085
Sau3AI GATC 7 cut(s) 171, 866, 1002, 1006, 1095, 1201, 1225
SchI GAGTC 3 cut(s) 104, 128, 974
ScrFI CCNGG 5 cut(s) 135, 190, 864, 933, 1263
SduI GDGCHC 2 cut(s) 196, 991
SfaNI GCATC 5 cut(s) 545, 558, 981, 1174, 1303
SfcI CTRYAG 2 cut(s) 543, 1036
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 3 cut(s) 92, 771, 1110
SmoI CTYRAG 3 cut(s) 92, 771, 1110
SsiI CCGC 6 cut(s) 345, 392, 588, 966, 1023, 1187
SspI AATATT 1 cut(s) 835
SspMI CTAG 2 cut(s) 335, 920
StyD4I CCNGG 5 cut(s) 133, 188, 862, 931, 1261
StyI CCWWGG 2 cut(s) 558, 1018
TaaI ACNGT 4 cut(s) 289, 458, 476, 527
TaiI ACGT 4 cut(s) 256, 648, 745, 1319
TaqI TCGA 7 cut(s) 162, 174, 416, 539, 582, 601, 1005
TauI GCSGC 1 cut(s) 969
TfiI GAWTC 3 cut(s) 159, 413, 704
Tru1I TTAA 7 cut(s) 26, 380, 519, 884, 1149, 1332, 1375
Tru9I TTAA 7 cut(s) 26, 380, 519, 884, 1149, 1332, 1375
TscAI CASTG 3 cut(s) 346, 371, 996
TseFI GTSAC 2 cut(s) 730, 1312
TseI GCWGC 5 cut(s) 5, 8, 450, 969, 1084
Tsp45I GTSAC 2 cut(s) 730, 1312
TspDTI ATGAA 1 cut(s) 696
TspRI CASTG 3 cut(s) 346, 371, 996
VneI GTGCAC 1 cut(s) 987
XapI RAATTY 2 cut(s) 886, 1216
XbaI TCTAGA 1 cut(s) 334
XmiI GTMKAC 3 cut(s) 510, 582, 1279
XmnI GAANNNNTTC 1 cut(s) 743
XspI CTAG 2 cut(s) 335, 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.