Prupe.4G207600_v2.0.a1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0017643
Physical Location & Seq
Reverse (-)
12923231 .. 12924610
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G207600.1

Sequence Viewer

Length: 1380 bp
ATGGCTGGAGTCTCATCTGTTCTTGACATAACTCTCTTTCTTTGTCTCATCACAATCTCTGCATTCCATCCAACATATTCAAAACCAAATGGTTTCAGCCTCAAGCTCATCCCCAGAGACGCTCCTGAATCTCCTTTATACCCTGGAAACCTCACTCTACTAGAACGAATTCAAAGAATGATCAAGTTCTCCGAAGCCAGATCTCACTATTTGGACTTAATTTCATCTTCAAATTCCACCGTAGAAATTGACAATATAAGTCTTACTTTGCTTCGTGACAACTTCTTCTACATGGTGCAAGTCGGCATTGGATCCCCAGCCACACTTGTGTTCCTACTTTTGGATACCGGCGGCAGCCTAATTTGGACGCAGTGCAAACCTTGTCAAAATTGTTACATACAAGCAACTCCCATTTACGATTCAACACAATCATGGAGTTATCGAAAGCTTGCTTGTAACCACCCCTTTTGTCAAGGTCCCCGGGCCCTTTACCAATGTGTTAACGATGAATGTGTCTACAACATCAATTATGGTGGTGGGGCATTGACTAGAGGTATAGCTTCTATTGAAATATTTACATTTCCATTTAACATCACAACCAGTGTACGCATCGCTGACATAGTCTTTGGTTGTTCGAACGACAATCGAAACATTCAATTTGCGAAAAATGGTGTCATTTCGGGGGTGTTGGGGTTAAGCTTGAACCCTGATTCACTGTTGTCCCAGTTGGCTGATCAAACCCAAAGACGTTTCTCATATTGCTTGGTTCCTTTTACTGAAGCACAAATGGCTCCTAGTGTGGTAAGGTTTGGGGATGATATACCCGCACCACCTGGAGGTATTAGCACAACTCCTTTTGTTACACGACCAGGATCTCCATTTTACAACTTAAATCTGTTGGATATAAGTGTTGGATCACGCAAGTTAGGGTTTCCACCGGGTACCTTTGCATATAGTCAAAGTGGTACCCGTGGTTTCTTCATTGATTCAGGTGCCCTAATCTCTCAACTTGATCAAAATGCCAATGGACGTAACACGTACCGGGAGGTTATGGGGGCATTTCAAAACTATTATGATTCTCTCCATCTTACAAGAATTGGTCGAGTGCCTGAAGGGTTTCAATTGTGTTACAGCTATACGCCGCAATTTACTCAATTTGCCTCCATGACATACCATTTTGAGGGTTCTAATTATGTTGTTGATCCAAGATATGTGAACTTTTATAATCATGAGGCAGGTTATTTTTGTGTAGCAATGATGCCAGGAAATGGGAAATCTATACTAGGAGCATGGCATCAGCAAAATATGAGGCTCATCTACGATCTCAACACCCATGCGCTCCAATTTACTCCTGAAATTTGTGCTAACGACAATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

460

Amino Acids

51.05

Weight (kDa)

6.24

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017643)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07191
malus_domestica MD11G1223600.v1.1
prunus_persica Prupe.4G207600_v2.0.a1 Prupe.4G207700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399441
rosa_laevigata RLG00000009268
rosa_roxburghii Rroxscaffold_5G00344340
rosa_rugosa Rorug04G0016900
rosa_samantha Rh4BG092000 Rh4CG102900 Rh4DG086900
rosa_wichuraiana Rw4G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1224
Acc36I ACCTGC 1 cut(s) 1226
Acc65I GGTACC 2 cut(s) 941, 965
AccB1I GGYRCC 3 cut(s) 941, 965, 992
AccB7I CCANNNNNTGG 1 cut(s) 1268
AccI GTMKAC 1 cut(s) 516
AciI CCGC 3 cut(s) 351, 825, 1142
AclWI GGATC 5 cut(s) 306, 319, 880, 922, 1196
AcsI RAATTY 3 cut(s) 168, 232, 1356
AcuI CTGAAG 2 cut(s) 798, 1131
AfaI GTAC 4 cut(s) 606, 943, 967, 1040
AfiI CCNNNNNNNGG 4 cut(s) 340, 836, 1180, 1268
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 9 cut(s) 81, 173, 231, 423, 569, 656, 703, 1064, 1121
AjnI CCWGG 4 cut(s) 142, 832, 868, 1261
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 5 cut(s) 106, 448, 560, 699, 1134
AluI AGCT 5 cut(s) 106, 448, 560, 699, 1134
Alw26I GTCTC 3 cut(s) 16, 50, 111
AlwI GGATC 5 cut(s) 306, 319, 880, 922, 1196
Ama87I CYCGRG 1 cut(s) 480
AoxI GGCC 1 cut(s) 483
ApaI GGGCCC 1 cut(s) 487
ApeKI GCWGC 1 cut(s) 354
ApoI RAATTY 3 cut(s) 168, 232, 1356
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
Asp700I GAANNNNTTC 2 cut(s) 168, 1116
Asp718I GGTACC 2 cut(s) 941, 965
AspLEI GCGC 1 cut(s) 1339
AspS9I GGNCC 3 cut(s) 476, 483, 484
AsuC2I CCSGG 4 cut(s) 481, 482, 939, 1043
AsuII TTCGAA 1 cut(s) 635
AvaI CYCGRG 1 cut(s) 480
AvaII GGWCC 1 cut(s) 476
BaeGI GKGCMC 2 cut(s) 487, 997
BamHI GGATCC 1 cut(s) 311
BanI GGYRCC 3 cut(s) 941, 965, 992
BanII GRGCYC 1 cut(s) 487
BbvI GCAGC 1 cut(s) 366
BccI CCATC 2 cut(s) 75, 1092
BciT130I CCWGG 4 cut(s) 144, 834, 870, 1263
BciVI GTATCC 1 cut(s) 337
BclI TGATCA 3 cut(s) 180, 733, 1012
BcnI CCSGG 4 cut(s) 481, 482, 939, 1043
BcoDI GTCTC 3 cut(s) 16, 50, 111
BfaI CTAG 4 cut(s) 161, 549, 795, 1283
BfuAI ACCTGC 1 cut(s) 1226
BfuI GTATCC 1 cut(s) 337
BglII AGATCT 1 cut(s) 200
BisI GCNGC 3 cut(s) 352, 355, 1142
BlsI GCNGC 3 cut(s) 353, 356, 1143
Bme1390I CCNGG 8 cut(s) 144, 481, 482, 834, 870, 939, 1043, 1263
Bme18I GGWCC 1 cut(s) 476
BmeT110I CYCGRG 1 cut(s) 480
BmgT120I GGNCC 3 cut(s) 476, 483, 484
BmiI GGNNCC 8 cut(s) 313, 478, 485, 768, 792, 943, 967, 994
BmrFI CCNGG 8 cut(s) 144, 481, 482, 834, 870, 939, 1043, 1263
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 3 cut(s) 618, 1248, 1303
BmuI ACTGGG 1 cut(s) 718
BpmI CTGGAG 2 cut(s) 27, 855
Bpu14I TTCGAA 1 cut(s) 635
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 4 cut(s) 481, 482, 939, 1043
BsaAI YACGTR 1 cut(s) 1038
BsaJI CCNNGG 4 cut(s) 142, 479, 480, 970
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 836, 1180, 1268
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 2 cut(s) 600, 724
Bse3DI GCAATG 1 cut(s) 1260
BseBI CCWGG 4 cut(s) 144, 834, 870, 1263
BseDI CCNNGG 4 cut(s) 142, 479, 480, 970
BseGI GGATG 3 cut(s) 67, 108, 820
BseLI CCNNNNNNNGG 4 cut(s) 340, 836, 1180, 1268
BseMI GCAATG 1 cut(s) 1260
BseNI ACTGG 2 cut(s) 600, 724
BseSI GKGCMC 2 cut(s) 487, 997
BseXI GCAGC 1 cut(s) 366
BseYI CCCAGC 1 cut(s) 316
BshFI GGCC 1 cut(s) 485
BshNI GGYRCC 3 cut(s) 941, 965, 992
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 4 cut(s) 348, 481, 938, 1042
BslFI GGGAC 2 cut(s) 462, 706
BslI CCNNNNNNNGG 4 cut(s) 340, 836, 1180, 1268
BsmAI GTCTC 3 cut(s) 16, 50, 111
BsmBI CGTCTC 1 cut(s) 111
BsmFI GGGAC 2 cut(s) 462, 706
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 1 cut(s) 485
BsoBI CYCGRG 1 cut(s) 480
Bsp119I TTCGAA 1 cut(s) 635
Bsp120I GGGCCC 1 cut(s) 483
Bsp1286I GDGCHC 2 cut(s) 487, 997
Bsp143I GATC 9 cut(s) 180, 200, 311, 733, 872, 914, 1012, 1201, 1321
BspACI CCGC 3 cut(s) 351, 825, 1142
BspANI GGCC 1 cut(s) 485
BspHI TCATGA 1 cut(s) 1228
BspLI GGNNCC 8 cut(s) 313, 478, 485, 768, 792, 943, 967, 994
BspMI ACCTGC 1 cut(s) 1226
BspPI GGATC 5 cut(s) 306, 319, 880, 922, 1196
BspT104I TTCGAA 1 cut(s) 635
BspT107I GGYRCC 3 cut(s) 941, 965, 992
BsrDI GCAATG 1 cut(s) 1260
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 2 cut(s) 600, 724
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 4 cut(s) 142, 479, 480, 970
BssMI GATC 9 cut(s) 180, 200, 311, 733, 872, 914, 1012, 1201, 1321
Bst2UI CCWGG 4 cut(s) 144, 834, 870, 1263
Bst4CI ACNGT 2 cut(s) 241, 717
BstBAI YACGTR 1 cut(s) 1038
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 450
BstDSI CCRYGG 1 cut(s) 970
BstF5I GGATG 3 cut(s) 67, 108, 820
BstHHI GCGC 1 cut(s) 1339
BstKTI GATC 9 cut(s) 183, 203, 314, 736, 875, 917, 1015, 1204, 1324
BstMAI GTCTC 3 cut(s) 16, 50, 111
BstMBI GATC 9 cut(s) 180, 200, 311, 733, 872, 914, 1012, 1201, 1321
BstMWI GCNNNNNNNGC 1 cut(s) 788
BstNI CCWGG 4 cut(s) 144, 834, 870, 1263
BstSCI CCNGG 8 cut(s) 142, 479, 480, 832, 868, 937, 1041, 1261
BstSLI GKGCMC 2 cut(s) 487, 997
BstV1I GCAGC 1 cut(s) 366
BstX2I RGATCY 3 cut(s) 200, 311, 872
BstYI RGATCY 3 cut(s) 200, 311, 872
BsuI GTATCC 1 cut(s) 337
BsuRI GGCC 1 cut(s) 485
BtgI CCRYGG 1 cut(s) 970
BtgZI GCGATG 1 cut(s) 595
BtsCI GGATG 3 cut(s) 67, 108, 820
BtsI GCAGTG 1 cut(s) 377
BtsIMutI CAGTG 3 cut(s) 377, 607, 713
BveI ACCTGC 1 cut(s) 1226
Cac8I GCNNGC 1 cut(s) 450
CciI TCATGA 1 cut(s) 1228
CfoI GCGC 1 cut(s) 1339
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 3 cut(s) 476, 483, 484
Cfr9I CCCGGG 1 cut(s) 480
CseI GACGC 2 cut(s) 128, 376
Csp6I GTAC 4 cut(s) 605, 942, 966, 1039
CspCI CAANNNNNGTGG 4 cut(s) 449, 484, 514, 549
CviAII CATG 6 cut(s) 292, 432, 1165, 1229, 1290, 1334
CviQI GTAC 4 cut(s) 605, 942, 966, 1039
DpnI GATC 9 cut(s) 182, 202, 313, 735, 874, 916, 1014, 1203, 1323
DpnII GATC 9 cut(s) 180, 200, 311, 733, 872, 914, 1012, 1201, 1321
Eco24I GRGCYC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 476
Eco57I CTGAAG 2 cut(s) 798, 1131
Eco88I CYCGRG 1 cut(s) 480
EcoO109I RGGNCCY 2 cut(s) 476, 484
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 4 cut(s) 142, 832, 868, 1261
EcoT38I GRGCYC 1 cut(s) 487
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 6 cut(s) 295, 435, 1168, 1232, 1293, 1337
FalI AAGNNNNNCTT 2 cut(s) 250, 282
FaqI GGGAC 2 cut(s) 462, 706
FatI CATG 6 cut(s) 291, 431, 1164, 1228, 1289, 1333
FauI CCCGC 1 cut(s) 832
FbaI TGATCA 3 cut(s) 180, 733, 1012
FblI GTMKAC 1 cut(s) 516
Fnu4HI GCNGC 3 cut(s) 352, 355, 1142
FokI GGATG 3 cut(s) 54, 95, 827
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 3 cut(s) 352, 355, 1142
FspBI CTAG 4 cut(s) 161, 549, 795, 1283
GlaI GCGC 1 cut(s) 1338
GluI GCNGC 3 cut(s) 352, 355, 1142
GsaI CCCAGC 1 cut(s) 320
GsuI CTGGAG 2 cut(s) 27, 855
HaeIII GGCC 1 cut(s) 485
HapII CCGG 4 cut(s) 348, 481, 938, 1042
HgaI GACGC 2 cut(s) 128, 376
HhaI GCGC 1 cut(s) 1339
Hin1II CATG 6 cut(s) 295, 435, 1168, 1232, 1293, 1337
Hin6I GCGC 1 cut(s) 1337
HinP1I GCGC 1 cut(s) 1337
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 2 cut(s) 446, 697
HinfI GANTC 6 cut(s) 9, 128, 419, 710, 986, 1076
HpaI GTTAAC 1 cut(s) 502
HpaII CCGG 4 cut(s) 348, 481, 938, 1042
Hpy166II GTNNAC 4 cut(s) 502, 517, 605, 1216
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 5 cut(s) 23, 125, 275, 1229, 1352
Hpy8I GTNNAC 4 cut(s) 502, 517, 605, 1216
HpyAV CCTTC 1 cut(s) 1106
HpyCH4III ACNGT 2 cut(s) 241, 717
HpyCH4IV ACGT 3 cut(s) 748, 1030, 1037
HpyCH4V TGCA 4 cut(s) 62, 298, 375, 950
HpyF10VI GCNNNNNNNGC 1 cut(s) 788
HpySE526I ACGT 3 cut(s) 748, 1030, 1037
Hsp92II CATG 6 cut(s) 295, 435, 1168, 1232, 1293, 1337
HspAI GCGC 1 cut(s) 1337
KpnI GGTACC 2 cut(s) 945, 969
Ksp22I TGATCA 3 cut(s) 180, 733, 1012
KspAI GTTAAC 1 cut(s) 502
Kzo9I GATC 9 cut(s) 180, 200, 311, 733, 872, 914, 1012, 1201, 1321
LmnI GCTCC 4 cut(s) 127, 796, 1286, 1344
Lsp1109I GCAGC 1 cut(s) 366
LweI GCATC 3 cut(s) 618, 1248, 1303
MaeI CTAG 4 cut(s) 161, 549, 795, 1283
MaeII ACGT 3 cut(s) 748, 1030, 1037
MaeIII GTNAC 6 cut(s) 275, 392, 455, 859, 1031, 1127
MalI GATC 9 cut(s) 182, 202, 313, 735, 874, 916, 1014, 1203, 1323
MboI GATC 9 cut(s) 180, 200, 311, 733, 872, 914, 1012, 1201, 1321
MboII GAAGA 3 cut(s) 219, 277, 970
MfeI CAATTG 1 cut(s) 1121
MflI RGATCY 3 cut(s) 200, 311, 872
MhlI GDGCHC 2 cut(s) 487, 997
MlyI GAGTC 1 cut(s) 18
MmeI TCCRAC 3 cut(s) 95, 879, 892
MnlI CCTC 9 cut(s) 110, 161, 545, 830, 1039, 1171, 1174, 1225, 1302
MroXI GAANNNNTTC 2 cut(s) 168, 1116
MseI TTAA 6 cut(s) 218, 501, 588, 695, 890, 1378
MslI CAYNNNNRTG 2 cut(s) 326, 430
MspI CCGG 4 cut(s) 348, 481, 938, 1042
MspR9I CCNGG 8 cut(s) 144, 481, 482, 834, 870, 939, 1043, 1263
MunI CAATTG 1 cut(s) 1121
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 4 cut(s) 144, 834, 870, 1263
MwoI GCNNNNNNNGC 1 cut(s) 788
NciI CCSGG 4 cut(s) 481, 482, 939, 1043
NdeII GATC 9 cut(s) 180, 200, 311, 733, 872, 914, 1012, 1201, 1321
NlaIII CATG 6 cut(s) 295, 435, 1168, 1232, 1293, 1337
NlaIV GGNNCC 8 cut(s) 313, 478, 485, 768, 792, 943, 967, 994
NmuCI GTSAC 1 cut(s) 275
NspV TTCGAA 1 cut(s) 635
OliI CACNNNNGTG 1 cut(s) 326
PagI TCATGA 1 cut(s) 1228
PctI GAATGC 1 cut(s) 62
PdmI GAANNNNTTC 2 cut(s) 168, 1116
PfeI GAWTC 5 cut(s) 128, 419, 710, 986, 1076
PflFI GACNNNGTC 1 cut(s) 620
PflMI CCANNNNNTGG 1 cut(s) 1268
PkrI GCNGC 3 cut(s) 353, 356, 1143
PleI GAGTC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 17
Ppu21I YACGTR 1 cut(s) 1038
PpuMI RGGWCCY 1 cut(s) 476
PsiI TTATAA 1 cut(s) 1224
Psp5II RGGWCCY 1 cut(s) 476
Psp6I CCWGG 4 cut(s) 142, 832, 868, 1261
PspFI CCCAGC 1 cut(s) 316
PspGI CCWGG 4 cut(s) 142, 832, 868, 1261
PspN4I GGNNCC 8 cut(s) 313, 478, 485, 768, 792, 943, 967, 994
PspOMI GGGCCC 1 cut(s) 483
PspPI GGNCC 3 cut(s) 476, 483, 484
PspPPI RGGWCCY 1 cut(s) 476
PsuI RGATCY 3 cut(s) 200, 311, 872
PsyI GACNNNGTC 1 cut(s) 620
RsaI GTAC 4 cut(s) 606, 943, 967, 1040
RsaNI GTAC 4 cut(s) 605, 942, 966, 1039
RseI CAYNNNNRTG 2 cut(s) 326, 430
SaqAI TTAA 6 cut(s) 218, 501, 588, 695, 890, 1378
SatI GCNGC 3 cut(s) 352, 355, 1142
Sau3AI GATC 9 cut(s) 180, 200, 311, 733, 872, 914, 1012, 1201, 1321
Sau96I GGNCC 3 cut(s) 476, 483, 484
SchI GAGTC 1 cut(s) 18
ScrFI CCNGG 8 cut(s) 144, 481, 482, 834, 870, 939, 1043, 1263
SduI GDGCHC 2 cut(s) 487, 997
SfaNI GCATC 3 cut(s) 618, 1248, 1303
SfuI TTCGAA 1 cut(s) 635
SinI GGWCC 1 cut(s) 476
SmaI CCCGGG 1 cut(s) 482
SmiMI CAYNNNNRTG 2 cut(s) 326, 430
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SsiI CCGC 3 cut(s) 351, 825, 1142
SspI AATATT 1 cut(s) 573
SspMI CTAG 4 cut(s) 161, 549, 795, 1283
StyD4I CCNGG 8 cut(s) 142, 479, 480, 832, 868, 937, 1041, 1261
TaaI ACNGT 2 cut(s) 241, 717
TaiI ACGT 3 cut(s) 751, 1033, 1040
TaqI TCGA 4 cut(s) 442, 635, 646, 1102
TauI GCSGC 2 cut(s) 354, 1144
TfiI GAWTC 5 cut(s) 128, 419, 710, 986, 1076
Tru1I TTAA 6 cut(s) 218, 501, 588, 695, 890, 1378
Tru9I TTAA 6 cut(s) 218, 501, 588, 695, 890, 1378
TscAI CASTG 3 cut(s) 377, 607, 720
TseFI GTSAC 1 cut(s) 275
TseI GCWGC 1 cut(s) 354
Tsp45I GTSAC 1 cut(s) 275
TspDTI ATGAA 3 cut(s) 213, 522, 970
TspMI CCCGGG 1 cut(s) 480
TspRI CASTG 3 cut(s) 377, 607, 720
Tth111I GACNNNGTC 1 cut(s) 620
Van91I CCANNNNNTGG 1 cut(s) 1268
VpaK11BI GGWCC 1 cut(s) 476
XapI RAATTY 3 cut(s) 168, 232, 1356
XmaI CCCGGG 1 cut(s) 480
XmiI GTMKAC 1 cut(s) 516
XmnI GAANNNNTTC 2 cut(s) 168, 1116
XspI CTAG 4 cut(s) 161, 549, 795, 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.