Rroxscaffold_5G00344340

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017643
Physical Location & Seq
Reverse (-)
14373534 .. 14380749
7216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00344340.1

Sequence Viewer

Length: 1839 bp
ATGCCGACGCTGAACCTGTTCACGAATGTTCCAGTGGATGCAGTGATAGCCTCTGACATTCTCAAGGACGCCACCAAAGCCGTCGCCAAAATCATCGGCAAACCCGAGTCCGTTAAGTCTCTTCCTTTCCTCCCTCTCTCATCTTCCATCTTTTCTCTTGTTCTGGGTGTGCACACAAGGTGTTCGATGAAATATGTGATGATTTTGCTCAATGGGAGCGTGCCGATTGCGTTTGCCGGCACGGAAGAGCCCGCTGCGTATGGGGAGTTGATCTCCATTGGAGGTATTGGACCCAGTGTTAATGGGAAGCTGAGTTCAACTATTGCGGAGATTCTGGAGACCAAGCTTTCAATAGATAGCTCCCGCTTCTACATCAAATTTTATGACGTTCAGCCTCAAGCTCATCCCCAGAGACTCCCCCGAGTCTCCTCTGTACCAGGAAACCTCACTACACTTGAACGAATCGAAAGATTGATCGAGTTCTCCCGAGCCAGGGCTCGCTATGTGGAACTAACTTCGTCTCCAAATGCAACCATCTTGTATCCCGAAAACATACGTCTCACTTTGCTTCGTGATAACTTCTTCTACACGGTGCAGGTAGGTGTTGGAACTCCAGCCACAGGAGTGTTCCTGCTTCTAGACACTGGCGGTGGCCTAATCTGGACGCAGTGCCTACCTTGCTTAAATTGCTACCCGCAAAGAACTCCCATGTTCGATTCGAGTAAATCCAGCACTTATCAAAAGCTTCCTTGCAGCCACCCTTTTTGTCAAGGCGACCGTCCCCTCTACAAATGTGTCAATGATGAGTGTGTCTACAACATTAACTACGGTGGAGGGGCATCGACTGCAGGCATCGCTTCCATGGAAACATTCACATTTCCAGTCGACACCGCCACTACTACTCGAATTGGTCAAATTGTTTTTGGTTGCTCAAAGTATAGCCGAAACGTTCAATTTGCCAAAGGTGGTGTCATTTCAGGGGTCATGGGATTGAGCTTGTCTCCAGATTCATTGGTTCCACAGTTGTCTGATGTGACACACAAACGTTTCTCATATTGCTTGGTACCTTTCACTCAAGCACAGCAGGCACCAAGTCTGGTAAGGTTTGGGGATGATATTCCCCACCCATCCTCGAATATTCAGACAACTCCTTTTGTACCAACACCCAGATCCCATTACTTTCAATTAAATTTGTTGGACATAAGTGTCGGATATCGCAAGCTAGGGTTTCCCCCAGGTACCTTTACGGCGAGGCCAGATGGTTCAGGCGGCTGCATCGTTGACTCCGGTGCACTGATACCTCAGATCGATCAACATGCCCAAGGCGGACGCAACGCCTACAGGCAGGTGATGTCTGCGTTTCAGAGCTATTATAATTCTTTCCAGCAGCTTACGAGGATCGGTAAAGTGCCTCAAGGGTTTGCATTGTGTTACAAGTATCCCCAAGATTTTAATCAATTTACCAGCATGACATATCATTTTGAGGATGCGGATTATATTGTGGATCCGAAATATGTGAATTTTTATGATCAGCAGGGAGGCTACTTTTGTGTTGCGTTGTTGCCTGGAAATGGGAAGAGTATACTTGGAGCATGGCATCAGCAAAATATGAGGGTGACGTACGATGGCAACATTAACTCACTCCAATTCTCTGTGGAGACTTGTTCTTATGATGATATTCCAGGAGCAAGACTTGTTCATGCTCCTCTTTTATGTAATTCAATAGCACATAGACTTGCTGCTATTGCTTTTGAAACTTTCCAGCAAGAGGTCTGGTTCCATCAGCCTCCTAGACTTATACTCGATGTACTGCAGTATGACTCTACTCATACTACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

612

Amino Acids

67.49

Weight (kDa)

7.51

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF PF01187 64 - 133 1.2e-09 Macrophage migration inhibitory factor (MIF)
TAXi_N PF14543 196 - 371 3.1e-42 Xylanase inhibitor N-terminal
TAXi_C PF14541 396 - 551 1.4e-15 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017643)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07191
malus_domestica MD11G1223600.v1.1
prunus_persica Prupe.4G207600_v2.0.a1 Prupe.4G207700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399441
rosa_laevigata RLG00000009268
rosa_roxburghii Rroxscaffold_5G00344340
rosa_rugosa Rorug04G0016900
rosa_samantha Rh4BG092000 Rh4CG102900 Rh4DG086900
rosa_wichuraiana Rw4G007780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1374
AarI CACCTGC 1 cut(s) 1336
AasI GACNNNNNNGTC 1 cut(s) 1205
Acc36I ACCTGC 2 cut(s) 586, 1336
Acc65I GGTACC 2 cut(s) 1063, 1238
AccB1I GGYRCC 3 cut(s) 1063, 1087, 1238
AccI GTMKAC 3 cut(s) 813, 885, 1582
AciI CCGC 9 cut(s) 252, 326, 364, 648, 695, 891, 1269, 1326, 1490
AclI AACGTT 2 cut(s) 948, 1045
AclWI GGATC 4 cut(s) 1164, 1406, 1499, 1512
AcsI RAATTY 3 cut(s) 377, 1189, 1519
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 180
AfaI GTAC 6 cut(s) 435, 1065, 1158, 1240, 1622, 1809
AfiI CCNNNNNNNGG 5 cut(s) 492, 493, 620, 1571, 1768
AgsI TTSAA 7 cut(s) 318, 351, 458, 953, 1184, 1722, 1754
AjnI CCWGG 5 cut(s) 436, 491, 1234, 1564, 1681
AleI CACNNNNGTG 1 cut(s) 623
AloI GAACNNNNNNTCC 2 cut(s) 298, 330
AluBI AGCT 9 cut(s) 310, 346, 360, 401, 745, 996, 1222, 1368, 1390
AluI AGCT 9 cut(s) 310, 346, 360, 401, 745, 996, 1222, 1368, 1390
Alw21I GWGCWC 2 cut(s) 174, 1294
Alw26I GTCTC 8 cut(s) 123, 332, 406, 430, 525, 563, 1005, 1652
Alw44I GTGCAC 2 cut(s) 170, 1290
AlwI GGATC 4 cut(s) 1164, 1406, 1499, 1512
Ama87I CYCGRG 3 cut(s) 104, 420, 486
AoxI GGCC 2 cut(s) 652, 1253
ApaLI GTGCAC 2 cut(s) 170, 1290
ApeKI GCWGC 5 cut(s) 254, 753, 1272, 1387, 1739
ApoI RAATTY 3 cut(s) 377, 1189, 1519
ArsI GACNNNNNNTTYG 2 cut(s) 954, 986
Asp700I GAANNNNTTC 1 cut(s) 17
Asp718I GGTACC 2 cut(s) 1063, 1238
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 2 cut(s) 1360, 1627
AvaI CYCGRG 3 cut(s) 104, 420, 486
AvaII GGWCC 1 cut(s) 290
BaeGI GKGCMC 2 cut(s) 174, 1294
BamHI GGATCC 1 cut(s) 1504
BanI GGYRCC 3 cut(s) 1063, 1087, 1238
BanII GRGCYC 2 cut(s) 252, 499
Bbv12I GWGCWC 2 cut(s) 174, 1294
BbvI GCAGC 5 cut(s) 241, 765, 1259, 1399, 1726
BccI CCATC 6 cut(s) 155, 542, 1135, 1253, 1619, 1788
BceAI ACGGC 2 cut(s) 65, 1263
BcgI CGANNNNNNTGC 2 cut(s) 1298, 1332
BciT130I CCWGG 5 cut(s) 438, 493, 1236, 1566, 1683
BciVI GTATCC 2 cut(s) 552, 1449
BclI TGATCA 1 cut(s) 1528
BcoDI GTCTC 8 cut(s) 123, 332, 406, 430, 525, 563, 1005, 1652
BfaI CTAG 3 cut(s) 638, 1223, 1791
BfmI CTRYAG 3 cut(s) 846, 1339, 1811
BfuAI ACCTGC 2 cut(s) 586, 1336
BfuI GTATCC 2 cut(s) 552, 1449
BisI GCNGC 6 cut(s) 255, 754, 1270, 1273, 1388, 1740
BlsI GCNGC 6 cut(s) 256, 755, 1271, 1274, 1389, 1741
Bme1390I CCNGG 5 cut(s) 438, 493, 1236, 1566, 1683
Bme18I GGWCC 1 cut(s) 290
BmeT110I CYCGRG 3 cut(s) 104, 420, 486
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 7 cut(s) 292, 1017, 1065, 1089, 1240, 1506, 1778
BmrFI CCNGG 5 cut(s) 438, 493, 1236, 1566, 1683
BmrI ACTGGG 1 cut(s) 288
BmsI GCATC 6 cut(s) 28, 848, 861, 1284, 1477, 1606
BmuI ACTGGG 1 cut(s) 288
BplI GAGNNNNNCTC 4 cut(s) 257, 289, 985, 1017
BpmI CTGGAG 3 cut(s) 356, 597, 987
BpuEI CTTGAG 4 cut(s) 47, 381, 1059, 1398
Bsa29I ATCGAT 1 cut(s) 1308
BsaBI GATNNNNATC 1 cut(s) 1452
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 4 cut(s) 492, 861, 1234, 1321
BsaWI WCCGGW 1 cut(s) 1286
BsaXI ACNNNNNCTCC 2 cut(s) 505, 535
Bsc4I CCNNNNNNNGG 5 cut(s) 492, 493, 620, 1571, 1768
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 4 cut(s) 32, 294, 649, 881
Bse8I GATNNNNATC 1 cut(s) 1452
BseBI CCWGG 5 cut(s) 438, 493, 1236, 1566, 1683
BseCI ATCGAT 1 cut(s) 1308
BseDI CCNNGG 4 cut(s) 492, 861, 1234, 1321
BseGI GGATG 5 cut(s) 43, 403, 1117, 1127, 1492
BseJI GATNNNNATC 1 cut(s) 1452
BseLI CCNNNNNNNGG 5 cut(s) 492, 493, 620, 1571, 1768
BseMII CTCAG 2 cut(s) 302, 1316
BseNI ACTGG 4 cut(s) 32, 294, 649, 881
BseRI GAGGAG 2 cut(s) 418, 1695
BseSI GKGCMC 2 cut(s) 174, 1294
BseXI GCAGC 5 cut(s) 241, 765, 1259, 1399, 1726
BsgI GTGCAG 1 cut(s) 614
Bsh1285I CGRYCG 1 cut(s) 778
BshFI GGCC 2 cut(s) 654, 1255
BshNI GGYRCC 3 cut(s) 1063, 1087, 1238
BshVI ATCGAT 1 cut(s) 1308
BsiEI CGRYCG 1 cut(s) 778
BsiHKAI GWGCWC 2 cut(s) 174, 1294
BsiHKCI CYCGRG 3 cut(s) 104, 420, 486
BsiSI CCGG 2 cut(s) 237, 1287
BsiWI CGTACG 1 cut(s) 1620
BslFI GGGAC 1 cut(s) 765
BslI CCNNNNNNNGG 5 cut(s) 492, 493, 620, 1571, 1768
BsmAI GTCTC 8 cut(s) 123, 332, 406, 430, 525, 563, 1005, 1652
BsmBI CGTCTC 2 cut(s) 525, 563
BsmFI GGGAC 1 cut(s) 765
BsnI GGCC 2 cut(s) 654, 1255
Bso31I GGTCTC 1 cut(s) 332
BsoBI CYCGRG 3 cut(s) 104, 420, 486
Bsp1286I GDGCHC 4 cut(s) 174, 252, 499, 1294
Bsp143I GATC 8 cut(s) 270, 474, 1169, 1305, 1309, 1398, 1504, 1528
Bsp19I CCATGG 1 cut(s) 861
BspACI CCGC 9 cut(s) 252, 326, 364, 648, 695, 891, 1269, 1326, 1490
BspANI GGCC 2 cut(s) 654, 1255
BspCNI CTCAG 2 cut(s) 303, 1315
BspDI ATCGAT 1 cut(s) 1308
BspLI GGNNCC 7 cut(s) 292, 1017, 1065, 1089, 1240, 1506, 1778
BspMAI CTGCAG 2 cut(s) 850, 1815
BspMI ACCTGC 2 cut(s) 586, 1336
BspPI GGATC 4 cut(s) 1164, 1406, 1499, 1512
BspQI GCTCTTC 1 cut(s) 240
BspT107I GGYRCC 3 cut(s) 1063, 1087, 1238
BspTNI GGTCTC 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 4 cut(s) 32, 294, 649, 881
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 4 cut(s) 492, 861, 1234, 1321
BssMI GATC 8 cut(s) 270, 474, 1169, 1305, 1309, 1398, 1504, 1528
BssNAI GTATAC 1 cut(s) 1583
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 861, 1321
Bst1107I GTATAC 1 cut(s) 1583
Bst2UI CCWGG 5 cut(s) 438, 493, 1236, 1566, 1683
Bst4CI ACNGT 4 cut(s) 592, 779, 830, 1023
Bst6I CTCTTC 3 cut(s) 126, 240, 1571
BstACI GRCGYC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 845
BstC8I GCNNGC 7 cut(s) 221, 238, 252, 499, 850, 1086, 1220
BstDEI CTNAG 3 cut(s) 311, 1302, 1836
BstDSI CCRYGG 1 cut(s) 861
BstF5I GGATG 5 cut(s) 43, 403, 1117, 1127, 1492
BstKTI GATC 8 cut(s) 273, 477, 1172, 1308, 1312, 1401, 1507, 1531
BstMAI GTCTC 8 cut(s) 123, 332, 406, 430, 525, 563, 1005, 1652
BstMBI GATC 8 cut(s) 270, 474, 1169, 1305, 1309, 1398, 1504, 1528
BstMCI CGRYCG 1 cut(s) 778
BstMWI GCNNNNNNNGC 8 cut(s) 47, 77, 678, 687, 845, 854, 1085, 1745
BstNI CCWGG 5 cut(s) 438, 493, 1236, 1566, 1683
BstNSI RCATGY 1 cut(s) 1319
BstSCI CCNGG 5 cut(s) 436, 491, 1234, 1564, 1681
BstSFI CTRYAG 3 cut(s) 846, 1339, 1811
BstSLI GKGCMC 2 cut(s) 174, 1294
BstV1I GCAGC 5 cut(s) 241, 765, 1259, 1399, 1726
BstX2I RGATCY 2 cut(s) 1169, 1504
BstYI RGATCY 2 cut(s) 1169, 1504
BstZ17I GTATAC 1 cut(s) 1583
Bsu15I ATCGAT 1 cut(s) 1308
BsuI GTATCC 2 cut(s) 552, 1449
BsuRI GGCC 2 cut(s) 654, 1255
BsuTUI ATCGAT 1 cut(s) 1308
BtgI CCRYGG 1 cut(s) 861
BtgZI GCGATG 1 cut(s) 838
BtsCI GGATG 5 cut(s) 43, 403, 1117, 1127, 1492
BtsI GCAGTG 2 cut(s) 48, 674
BtsIMutI CAGTG 6 cut(s) 39, 48, 301, 642, 674, 1292
BveI ACCTGC 2 cut(s) 586, 1336
Cac8I GCNNGC 7 cut(s) 221, 238, 252, 499, 850, 1086, 1220
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 290
ClaI ATCGAT 1 cut(s) 1308
CseI GACGC 4 cut(s) 16, 77, 673, 1338
Csp6I GTAC 6 cut(s) 434, 1064, 1157, 1239, 1621, 1808
CspCI CAANNNNNGTGG 2 cut(s) 746, 781
CviAII CATG 7 cut(s) 709, 862, 985, 1316, 1468, 1593, 1700
CviQI GTAC 6 cut(s) 434, 1064, 1157, 1239, 1621, 1808
DdeI CTNAG 3 cut(s) 311, 1302, 1836
DpnI GATC 8 cut(s) 272, 476, 1171, 1307, 1311, 1400, 1506, 1530
DpnII GATC 8 cut(s) 270, 474, 1169, 1305, 1309, 1398, 1504, 1528
DraIII CACNNNGTG 1 cut(s) 180
DrdI GACNNNNNNGTC 1 cut(s) 1205
DseDI GACNNNNNNGTC 1 cut(s) 1205
Eam1104I CTCTTC 3 cut(s) 126, 240, 1571
EarI CTCTTC 3 cut(s) 126, 240, 1571
EciI GGCGGA 1 cut(s) 1341
Eco130I CCWWGG 2 cut(s) 861, 1321
Eco24I GRGCYC 2 cut(s) 252, 499
Eco31I GGTCTC 1 cut(s) 332
Eco32I GATATC 1 cut(s) 1214
Eco47I GGWCC 1 cut(s) 290
Eco88I CYCGRG 3 cut(s) 104, 420, 486
EcoRII CCWGG 5 cut(s) 436, 491, 1234, 1564, 1681
EcoRV GATATC 1 cut(s) 1214
EcoT14I CCWWGG 2 cut(s) 861, 1321
EcoT38I GRGCYC 2 cut(s) 252, 499
ErhI CCWWGG 2 cut(s) 861, 1321
Esp3I CGTCTC 2 cut(s) 525, 563
FaeI CATG 7 cut(s) 712, 865, 988, 1319, 1471, 1596, 1703
FaqI GGGAC 1 cut(s) 765
FatI CATG 7 cut(s) 708, 861, 984, 1315, 1467, 1592, 1699
FauI CCCGC 3 cut(s) 259, 371, 702
FbaI TGATCA 1 cut(s) 1528
FblI GTMKAC 3 cut(s) 813, 885, 1582
Fnu4HI GCNGC 6 cut(s) 255, 754, 1270, 1273, 1388, 1740
FokI GGATG 5 cut(s) 50, 390, 1114, 1124, 1499
FriOI GRGCYC 2 cut(s) 252, 499
Fsp4HI GCNGC 6 cut(s) 255, 754, 1270, 1273, 1388, 1740
FspBI CTAG 3 cut(s) 638, 1223, 1791
GluI GCNGC 6 cut(s) 255, 754, 1270, 1273, 1388, 1740
GsuI CTGGAG 3 cut(s) 356, 597, 987
HaeIII GGCC 2 cut(s) 654, 1255
HapII CCGG 2 cut(s) 237, 1287
HgaI GACGC 4 cut(s) 16, 77, 673, 1338
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 7 cut(s) 712, 865, 988, 1319, 1471, 1596, 1703
HincII GTYRAC 2 cut(s) 886, 1282
HindII GTYRAC 2 cut(s) 886, 1282
HindIII AAGCTT 2 cut(s) 344, 743
HinfI GANTC 9 cut(s) 107, 331, 414, 423, 462, 716, 1007, 1283, 1820
HpaII CCGG 2 cut(s) 237, 1287
HphI GGTGA 2 cut(s) 1360, 1627
Hpy166II GTNNAC 7 cut(s) 21, 172, 814, 886, 1282, 1292, 1583
Hpy188I TCNGA 7 cut(s) 55, 1030, 1143, 1211, 1305, 1365, 1509
Hpy188III TCNNGA 8 cut(s) 22, 335, 486, 545, 572, 638, 661, 1004
Hpy8I GTNNAC 7 cut(s) 21, 172, 814, 886, 1282, 1292, 1583
Hpy99I CGWCG 2 cut(s) 10, 86
HpyCH4III ACNGT 4 cut(s) 592, 779, 830, 1023
HpyCH4IV ACGT 5 cut(s) 387, 556, 948, 1045, 1619
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 77, 678, 687, 845, 854, 1085, 1745
HpyF3I CTNAG 3 cut(s) 311, 1302, 1836
HpySE526I ACGT 5 cut(s) 387, 556, 948, 1045, 1619
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 7 cut(s) 712, 865, 988, 1319, 1471, 1596, 1703
KpnI GGTACC 2 cut(s) 1067, 1242
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 1528
Kzo9I GATC 8 cut(s) 270, 474, 1169, 1305, 1309, 1398, 1504, 1528
LguI GCTCTTC 1 cut(s) 240
LmnI GCTCC 5 cut(s) 216, 365, 1589, 1685, 1708
Lsp1109I GCAGC 5 cut(s) 241, 765, 1259, 1399, 1726
LweI GCATC 6 cut(s) 28, 848, 861, 1284, 1477, 1606
MaeI CTAG 3 cut(s) 638, 1223, 1791
MaeII ACGT 5 cut(s) 387, 556, 948, 1045, 1619
MaeIII GTNAC 3 cut(s) 1033, 1430, 1615
MalI GATC 8 cut(s) 272, 476, 1171, 1307, 1311, 1400, 1506, 1530
MboI GATC 8 cut(s) 270, 474, 1169, 1305, 1309, 1398, 1504, 1528
MboII GAAGA 5 cut(s) 113, 135, 257, 574, 1588
MflI RGATCY 2 cut(s) 1169, 1504
MhlI GDGCHC 4 cut(s) 174, 252, 499, 1294
MlyI GAGTC 5 cut(s) 116, 408, 432, 1277, 1814
MmeI TCCRAC 3 cut(s) 586, 1176, 1189
MroNI GCCGGC 1 cut(s) 236
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 7 cut(s) 114, 300, 683, 822, 1187, 1452, 1635
MslI CAYNNNNRTG 1 cut(s) 623
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 2 cut(s) 237, 1287
MspR9I CCNGG 5 cut(s) 438, 493, 1236, 1566, 1683
MvaI CCWGG 5 cut(s) 438, 493, 1236, 1566, 1683
MwoI GCNNNNNNNGC 8 cut(s) 47, 77, 678, 687, 845, 854, 1085, 1745
NaeI GCCGGC 1 cut(s) 238
NcoI CCATGG 1 cut(s) 861
NdeII GATC 8 cut(s) 270, 474, 1169, 1305, 1309, 1398, 1504, 1528
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 7 cut(s) 712, 865, 988, 1319, 1471, 1596, 1703
NlaIV GGNNCC 7 cut(s) 292, 1017, 1065, 1089, 1240, 1506, 1778
NmuCI GTSAC 2 cut(s) 1033, 1615
NspI RCATGY 1 cut(s) 1319
OliI CACNNNNGTG 1 cut(s) 623
PaqCI CACCTGC 1 cut(s) 1336
PciSI GCTCTTC 1 cut(s) 240
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PdiI GCCGGC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 4 cut(s) 331, 462, 716, 1007
Pfl23II CGTACG 1 cut(s) 1620
PfoI TCCNGGA 1 cut(s) 1681
PkrI GCNGC 6 cut(s) 256, 755, 1271, 1274, 1389, 1741
PleI GAGTC 5 cut(s) 115, 408, 431, 1277, 1814
PpsI GAGTC 5 cut(s) 115, 408, 431, 1277, 1814
PsiI TTATAA 1 cut(s) 1374
Psp1406I AACGTT 2 cut(s) 948, 1045
Psp6I CCWGG 5 cut(s) 436, 491, 1234, 1564, 1681
PspGI CCWGG 5 cut(s) 436, 491, 1234, 1564, 1681
PspLI CGTACG 1 cut(s) 1620
PspN4I GGNNCC 7 cut(s) 292, 1017, 1065, 1089, 1240, 1506, 1778
PspPI GGNCC 1 cut(s) 290
PstI CTGCAG 2 cut(s) 850, 1815
PsuI RGATCY 2 cut(s) 1169, 1504
RsaI GTAC 6 cut(s) 435, 1065, 1158, 1240, 1622, 1809
RsaNI GTAC 6 cut(s) 434, 1064, 1157, 1239, 1621, 1808
RseI CAYNNNNRTG 1 cut(s) 623
SalI GTCGAC 1 cut(s) 884
SapI GCTCTTC 1 cut(s) 240
SaqAI TTAA 7 cut(s) 114, 300, 683, 822, 1187, 1452, 1635
SatI GCNGC 6 cut(s) 255, 754, 1270, 1273, 1388, 1740
Sau3AI GATC 8 cut(s) 270, 474, 1169, 1305, 1309, 1398, 1504, 1528
Sau96I GGNCC 1 cut(s) 290
SchI GAGTC 5 cut(s) 116, 408, 432, 1277, 1814
ScrFI CCNGG 5 cut(s) 438, 493, 1236, 1566, 1683
SduI GDGCHC 4 cut(s) 174, 252, 499, 1294
SfaNI GCATC 6 cut(s) 28, 848, 861, 1284, 1477, 1606
SfcI CTRYAG 3 cut(s) 846, 1339, 1811
SinI GGWCC 1 cut(s) 290
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 4 cut(s) 62, 396, 1074, 1413
SmoI CTYRAG 4 cut(s) 62, 396, 1074, 1413
SsiI CCGC 9 cut(s) 252, 326, 364, 648, 695, 891, 1269, 1326, 1490
SspI AATATT 1 cut(s) 1138
SspMI CTAG 3 cut(s) 638, 1223, 1791
StyD4I CCNGG 5 cut(s) 436, 491, 1234, 1564, 1681
StyI CCWWGG 2 cut(s) 861, 1321
TaaI ACNGT 4 cut(s) 592, 779, 830, 1023
TaiI ACGT 5 cut(s) 390, 559, 951, 1048, 1622
TatI WGTACW 1 cut(s) 1807
TauI GCSGC 1 cut(s) 1272
TfiI GAWTC 4 cut(s) 331, 462, 716, 1007
Tru1I TTAA 7 cut(s) 114, 300, 683, 822, 1187, 1452, 1635
Tru9I TTAA 7 cut(s) 114, 300, 683, 822, 1187, 1452, 1635
TscAI CASTG 6 cut(s) 39, 48, 301, 649, 674, 1299
TseFI GTSAC 2 cut(s) 1033, 1615
TseI GCWGC 5 cut(s) 254, 753, 1272, 1387, 1739
Tsp45I GTSAC 2 cut(s) 1033, 1615
TspDTI ATGAA 3 cut(s) 203, 999, 1688
TspGWI ACGGA 2 cut(s) 100, 257
TspRI CASTG 6 cut(s) 39, 48, 301, 649, 674, 1299
VneI GTGCAC 2 cut(s) 170, 1290
VpaK11BI GGWCC 1 cut(s) 290
XapI RAATTY 3 cut(s) 377, 1189, 1519
XbaI TCTAGA 1 cut(s) 637
XceI RCATGY 1 cut(s) 1319
XmiI GTMKAC 3 cut(s) 813, 885, 1582
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 3 cut(s) 638, 1223, 1791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.