FvH4_4g08920

At4g14100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015595
Physical Location & Seq
Forward (+)
9882168 .. 9887459
5292 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g08920.t1

Sequence Viewer

Length: 519 bp
ATGAAGCAACTGAATCGACCGGTACAACTATGCTTAGCAACCTCAACCTGGCTAATCGTAGTGACTGGCTCAAACCTAACCCCAACACCTACACCATGGCCAGAGCAGTTTCACGCTCTTCTGTACATGAACCTCACTTCCTCAGGCCATCTCCAGCTTAGTGATGTATGGTACGACTGGCCTAGAGGCAGAAACGTAAACATATTTCAGAAGCAGCTGGGTGAGCTTTTGTATGATGTAGAGTGGAACAACGGCACGTCGTATTACTTCACCTTGGGGGAGGGAGGGCATTGCCAGGTTATGGAGTTTGAAGTGGGTATTCCTCGGCCTGATTTTCTTGATGGAGCAAACTACCTTGGGACAGAGGTCACTGATGGGTTTCTGTGCAACGTGTGGGAGAAGGTGGATTTCATTTGGTACTATGAAGATGTTCAAACTAAGAGACCTGTCCGTTGGGATTTCTATGATGGTCGTGAGGAGGCTCGAGCTGACGAGGCCCCAGATCGACATCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

20.05

Weight (kDa)

4.64

Isoelectric Point (pI)

35.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08920 FvH4_4g08920 FvH4_4g08920
malus_domestica MD13G1264800.v1.1
prunus_persica Prupe.1G092000_v2.0.a1
pyrus_communis pycom13g23700
rosa_chinensis RchiOBHm_Chr4g0403651
rosa_laevigata RLG00000008962
rosa_multiflora Rmu_co8223106.1_g000001
rosa_roxburghii Rroxscaffold_5G00348250
rosa_rugosa Rorug04G0044800
rosa_samantha Rh4AG119800 Rh4BG113100 Rh4CG128000 Rh4DG112500
rosa_wichuraiana Rw4G009730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 98
AfaI GTAC 4 cut(s) 24, 125, 173, 419
AfiI CCNNNNNNNGG 2 cut(s) 48, 301
AflIII ACRYGT 1 cut(s) 390
AgeI ACCGGT 1 cut(s) 19
AgsI TTSAA 2 cut(s) 311, 434
AjiI CACGTC 1 cut(s) 258
AjnI CCWGG 2 cut(s) 47, 294
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 4 cut(s) 157, 217, 226, 488
AluI AGCT 4 cut(s) 157, 217, 226, 488
Alw26I GTCTC 1 cut(s) 436
Ama87I CYCGRG 1 cut(s) 483
AoxI GGCC 5 cut(s) 98, 145, 179, 326, 495
ApeKI GCWGC 1 cut(s) 214
AsiGI ACCGGT 1 cut(s) 19
Asp700I GAANNNNTTC 1 cut(s) 429
AspS9I GGNCC 1 cut(s) 496
AsuHPI GGTGA 2 cut(s) 233, 262
AvaI CYCGRG 1 cut(s) 483
AxyI CCTNAGG 1 cut(s) 142
BalI TGGCCA 1 cut(s) 100
BbvI GCAGC 1 cut(s) 226
BccI CCATC 4 cut(s) 156, 335, 368, 461
BceAI ACGGC 1 cut(s) 268
BcgI CGANNNNNNTGC 1 cut(s) 30
BciT130I CCWGG 2 cut(s) 49, 296
BcoDI GTCTC 1 cut(s) 436
BfaI CTAG 1 cut(s) 183
BisI GCNGC 1 cut(s) 215
BlpI GCTNAGC 1 cut(s) 34
BlsI GCNGC 1 cut(s) 216
Bme1390I CCNGG 2 cut(s) 49, 296
BmeT110I CYCGRG 1 cut(s) 483
BmgBI CACGTC 1 cut(s) 258
BmgT120I GGNCC 1 cut(s) 496
BmiI GGNNCC 1 cut(s) 498
BmrFI CCNGG 2 cut(s) 49, 296
BoxI GACNNNNGTC 1 cut(s) 365
BpmI CTGGAG 1 cut(s) 137
Bpu1102I GCTNAGC 1 cut(s) 34
BsaBI GATNNNNATC 1 cut(s) 507
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 4 cut(s) 95, 273, 323, 355
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 301
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 70, 182
Bse21I CCTNAGG 1 cut(s) 142
Bse3DI GCAATG 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 507
BseBI CCWGG 2 cut(s) 49, 296
BseDI CCNNGG 4 cut(s) 95, 273, 323, 355
BseJI GATNNNNATC 1 cut(s) 507
BseLI CCNNNNNNNGG 2 cut(s) 48, 301
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 2 cut(s) 70, 182
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 1 cut(s) 226
BseYI CCCAGC 1 cut(s) 217
Bsh1285I CGRYCG 1 cut(s) 20
BshFI GGCC 5 cut(s) 100, 147, 181, 328, 497
BshTI ACCGGT 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 20
BsiHKCI CYCGRG 1 cut(s) 483
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 2 cut(s) 48, 301
BsmAI GTCTC 1 cut(s) 436
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 5 cut(s) 100, 147, 181, 328, 497
Bso31I GGTCTC 1 cut(s) 436
BsoBI CYCGRG 1 cut(s) 483
Bsp1407I TGTACA 1 cut(s) 123
Bsp143I GATC 1 cut(s) 502
Bsp1720I GCTNAGC 1 cut(s) 34
Bsp19I CCATGG 1 cut(s) 95
BspANI GGCC 5 cut(s) 100, 147, 181, 328, 497
BspCNI CTCAG 1 cut(s) 155
BspLI GGNNCC 1 cut(s) 498
BspQI GCTCTTC 1 cut(s) 123
BspTNI GGTCTC 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 19
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 2 cut(s) 70, 182
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 4 cut(s) 95, 273, 323, 355
BssMI GATC 1 cut(s) 502
BssT1I CCWWGG 3 cut(s) 95, 273, 355
Bst2UI CCWGG 2 cut(s) 49, 296
Bst6I CTCTTC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 123
BstDEI CTNAG 5 cut(s) 34, 142, 158, 438, 516
BstDSI CCRYGG 1 cut(s) 95
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 1 cut(s) 436
BstMBI GATC 1 cut(s) 502
BstMCI CGRYCG 1 cut(s) 20
BstMWI GCNNNNNNNGC 2 cut(s) 223, 494
BstNI CCWGG 2 cut(s) 49, 296
BstPAI GACNNNNGTC 1 cut(s) 365
BstSCI CCNGG 2 cut(s) 47, 294
BstV1I GCAGC 1 cut(s) 226
Bsu36I CCTNAGG 1 cut(s) 142
BsuRI GGCC 5 cut(s) 100, 147, 181, 328, 497
BtgI CCRYGG 1 cut(s) 95
BtrI CACGTC 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 369
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 1 cut(s) 496
Csp6I GTAC 4 cut(s) 23, 124, 172, 418
CspAI ACCGGT 1 cut(s) 19
CviAII CATG 2 cut(s) 96, 127
CviQI GTAC 4 cut(s) 23, 124, 172, 418
DdeI CTNAG 5 cut(s) 34, 142, 158, 438, 516
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
EaeI YGGCCR 1 cut(s) 98
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco130I CCWWGG 3 cut(s) 95, 273, 355
Eco31I GGTCTC 1 cut(s) 436
Eco81I CCTNAGG 1 cut(s) 142
Eco88I CYCGRG 1 cut(s) 483
EcoO109I RGGNCCY 1 cut(s) 496
EcoRII CCWGG 2 cut(s) 47, 294
EcoT14I CCWWGG 3 cut(s) 95, 273, 355
ErhI CCWWGG 3 cut(s) 95, 273, 355
FaeI CATG 2 cut(s) 99, 130
FaiI YATR 9 cut(s) 31, 97, 128, 169, 203, 234, 302, 423, 465
FaqI GGGAC 1 cut(s) 373
FatI CATG 2 cut(s) 95, 126
Fnu4HI GCNGC 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 215
FspBI CTAG 1 cut(s) 183
GluI GCNGC 1 cut(s) 215
GsaI CCCAGC 1 cut(s) 221
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 5 cut(s) 100, 147, 181, 328, 497
HapII CCGG 1 cut(s) 20
Hin1II CATG 2 cut(s) 99, 130
HinfI GANTC 1 cut(s) 13
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 233, 262
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 1 cut(s) 210
Hpy188III TCNNGA 2 cut(s) 338, 473
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 394
HpyCH4IV ACGT 3 cut(s) 195, 257, 390
HpyCH4V TGCA 1 cut(s) 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 494
HpyF3I CTNAG 5 cut(s) 34, 142, 158, 438, 516
HpySE526I ACGT 3 cut(s) 195, 257, 390
Hsp92II CATG 2 cut(s) 99, 130
Kzo9I GATC 1 cut(s) 502
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 1 cut(s) 344
Lsp1109I GCAGC 1 cut(s) 226
MaeI CTAG 1 cut(s) 183
MaeII ACGT 3 cut(s) 195, 257, 390
MaeIII GTNAC 2 cut(s) 61, 367
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 2 cut(s) 110, 437
MlsI TGGCCA 1 cut(s) 100
MluNI TGGCCA 1 cut(s) 100
Mox20I TGGCCA 1 cut(s) 100
MroXI GAANNNNTTC 1 cut(s) 429
MscI TGGCCA 1 cut(s) 100
Msp20I TGGCCA 1 cut(s) 100
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 49, 296
MvaI CCWGG 2 cut(s) 49, 296
MwoI GCNNNNNNNGC 2 cut(s) 223, 494
NcoI CCATGG 1 cut(s) 95
NdeII GATC 1 cut(s) 502
NlaIII CATG 2 cut(s) 99, 130
NlaIV GGNNCC 1 cut(s) 498
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 2 cut(s) 61, 367
PaeR7I CTCGAG 1 cut(s) 483
PciSI GCTCTTC 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 429
PfeI GAWTC 1 cut(s) 13
PflMI CCANNNNNTGG 1 cut(s) 301
PinAI ACCGGT 1 cut(s) 19
PkrI GCNGC 1 cut(s) 216
PshAI GACNNNNGTC 1 cut(s) 365
Psp6I CCWGG 2 cut(s) 47, 294
PspFI CCCAGC 1 cut(s) 217
PspGI CCWGG 2 cut(s) 47, 294
PspN4I GGNNCC 1 cut(s) 498
PspPI GGNCC 1 cut(s) 496
PspXI VCTCGAGB 1 cut(s) 483
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 4 cut(s) 24, 125, 173, 419
RsaNI GTAC 4 cut(s) 23, 124, 172, 418
SapI GCTCTTC 1 cut(s) 123
SatI GCNGC 1 cut(s) 215
Sau3AI GATC 1 cut(s) 502
Sau96I GGNCC 1 cut(s) 496
ScrFI CCNGG 2 cut(s) 49, 296
Sfr274I CTCGAG 1 cut(s) 483
SlaI CTCGAG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 483
SmoI CTYRAG 1 cut(s) 483
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 2 cut(s) 47, 294
StyI CCWWGG 3 cut(s) 95, 273, 355
TaiI ACGT 3 cut(s) 198, 260, 393
TaqI TCGA 3 cut(s) 16, 484, 505
TatI WGTACW 1 cut(s) 123
TfiI GAWTC 1 cut(s) 13
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 2 cut(s) 61, 367
TseI GCWGC 1 cut(s) 214
Tsp45I GTSAC 2 cut(s) 61, 367
TspDTI ATGAA 4 cut(s) 17, 143, 400, 438
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 1 cut(s) 376
Van91I CCANNNNNTGG 1 cut(s) 301
XhoI CTCGAG 1 cut(s) 483
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.