Rh4CG128000

At4g14100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0015595
Physical Location & Seq
Forward (+)
25128538 .. 25131938
3401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG128000.1

Sequence Viewer

Length: 447 bp
ATGAACATCACTTCCTCAGCCCATCTCCAGCTCAGTGATGTATGGTACGACTGGCCTAAAGGCCGAAACGTCAACATATTTCAGAAGCAGCTAGGTGAGCTGTTGTATGATATAGAGTGGAACAACGGCACGTCGTACTACTACACCTTGGGTGAGGGAGGACATTGCCAGGTTATGGCGTTTGAAGTGGGTATTCCTCGGCCTGATTTTCTTGATGGAGCAAACTACCTTGGGACTGAGGTCACTGATGGGTTTCTCTGCAATGTGTGGGAGAAGGTGGATTTCATTTGGTACTATGAGGATGTTCAGACTAAGAGGCCAGTTCGTTGGGATTTCTATGATGGAATAACTAGTCACGTCATGACATTTGAGACCCTCTCTCTCCTTTCCCTCAGGGAGGTACAGGGTTCTGCGTTCAAGATCCGATCTCAGACCCTTAACGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

148

Amino Acids

17.21

Weight (kDa)

4.7

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08920 FvH4_4g08920 FvH4_4g08920
malus_domestica MD13G1264800.v1.1
prunus_persica Prupe.1G092000_v2.0.a1
pyrus_communis pycom13g23700
rosa_chinensis RchiOBHm_Chr4g0403651
rosa_laevigata RLG00000008962
rosa_multiflora Rmu_co8223106.1_g000001
rosa_roxburghii Rroxscaffold_5G00348250
rosa_rugosa Rorug04G0044800
rosa_samantha Rh4AG119800 Rh4BG113100 Rh4CG128000 Rh4DG112500
rosa_wichuraiana Rw4G009730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 175
AclI AACGTT 1 cut(s) 441
AclWI GGATC 1 cut(s) 415
AfaI GTAC 4 cut(s) 47, 137, 293, 402
AfiI CCNNNNNNNGG 2 cut(s) 175, 397
AgsI TTSAA 2 cut(s) 185, 418
AhlI ACTAGT 1 cut(s) 350
AjiI CACGTC 2 cut(s) 132, 358
AjnI CCWGG 1 cut(s) 168
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 3 cut(s) 31, 91, 100
AluI AGCT 3 cut(s) 31, 91, 100
Alw26I GTCTC 1 cut(s) 365
AlwI GGATC 1 cut(s) 415
AoxI GGCC 4 cut(s) 53, 61, 200, 317
ApeKI GCWGC 1 cut(s) 88
AsuHPI GGTGA 2 cut(s) 107, 164
AxyI CCTNAGG 1 cut(s) 392
BbvCI CCTCAGC 1 cut(s) 16
BbvI GCAGC 1 cut(s) 100
BccI CCATC 4 cut(s) 30, 209, 242, 335
BceAI ACGGC 1 cut(s) 142
BciT130I CCWGG 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 365
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 2 cut(s) 92, 351
BisI GCNGC 1 cut(s) 89
BlsI GCNGC 1 cut(s) 90
Bme1390I CCNGG 1 cut(s) 170
BmgBI CACGTC 2 cut(s) 132, 358
BmrFI CCNGG 1 cut(s) 170
BoxI GACNNNNGTC 1 cut(s) 239
BplI GAGNNNNNCTC 2 cut(s) 362, 394
BpmI CTGGAG 1 cut(s) 11
Bpu10I CCTNAGC 1 cut(s) 16
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 3 cut(s) 147, 197, 229
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 397
Bse1I ACTGG 2 cut(s) 56, 320
Bse21I CCTNAGG 1 cut(s) 392
Bse3DI GCAATG 2 cut(s) 163, 268
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 3 cut(s) 147, 197, 229
BseGI GGATG 1 cut(s) 307
BseLI CCNNNNNNNGG 2 cut(s) 175, 397
BseMI GCAATG 2 cut(s) 163, 268
BseMII CTCAG 5 cut(s) 30, 46, 228, 406, 443
BseNI ACTGG 2 cut(s) 56, 320
BseXI GCAGC 1 cut(s) 100
BshFI GGCC 4 cut(s) 55, 63, 202, 319
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 2 cut(s) 175, 397
BsmAI GTCTC 1 cut(s) 365
BsmFI GGGAC 1 cut(s) 247
BsnI GGCC 4 cut(s) 55, 63, 202, 319
Bso31I GGTCTC 1 cut(s) 365
Bsp143I GATC 2 cut(s) 420, 425
BspANI GGCC 4 cut(s) 55, 63, 202, 319
BspCNI CTCAG 5 cut(s) 29, 45, 229, 405, 442
BspHI TCATGA 1 cut(s) 360
BspPI GGATC 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 2 cut(s) 163, 268
BsrI ACTGG 2 cut(s) 56, 320
BssECI CCNNGG 3 cut(s) 147, 197, 229
BssMI GATC 2 cut(s) 420, 425
BssT1I CCWWGG 2 cut(s) 147, 229
Bst2UI CCWGG 1 cut(s) 170
BstDEI CTNAG 6 cut(s) 16, 32, 237, 312, 392, 429
BstENI CCTNNNNNAGG 1 cut(s) 395
BstF5I GGATG 1 cut(s) 307
BstKTI GATC 2 cut(s) 423, 428
BstMAI GTCTC 1 cut(s) 365
BstMBI GATC 2 cut(s) 420, 425
BstMWI GCNNNNNNNGC 1 cut(s) 97
BstNI CCWGG 1 cut(s) 170
BstPAI GACNNNNGTC 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 100
BstX2I RGATCY 1 cut(s) 420
BstXI CCANNNNNNTGG 1 cut(s) 327
BstYI RGATCY 1 cut(s) 420
Bsu36I CCTNAGG 1 cut(s) 392
BsuRI GGCC 4 cut(s) 55, 63, 202, 319
BtrI CACGTC 2 cut(s) 132, 358
BtsCI GGATG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 40, 243
CciI TCATGA 1 cut(s) 360
Csp6I GTAC 4 cut(s) 46, 136, 292, 401
CviAII CATG 1 cut(s) 361
CviJI RGCY 8 cut(s) 20, 31, 55, 63, 91, 100, 202, 319
CviKI_1 RGCY 8 cut(s) 20, 31, 55, 63, 91, 100, 202, 319
CviQI GTAC 4 cut(s) 46, 136, 292, 401
DdeI CTNAG 6 cut(s) 16, 32, 237, 312, 392, 429
DpnI GATC 2 cut(s) 422, 427
DpnII GATC 2 cut(s) 420, 425
Eco130I CCWWGG 2 cut(s) 147, 229
Eco31I GGTCTC 1 cut(s) 365
Eco81I CCTNAGG 1 cut(s) 392
EcoNI CCTNNNNNAGG 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 168
EcoT14I CCWWGG 2 cut(s) 147, 229
ErhI CCWWGG 2 cut(s) 147, 229
FaeI CATG 1 cut(s) 364
FaiI YATR 8 cut(s) 43, 77, 108, 113, 176, 297, 339, 362
FaqI GGGAC 1 cut(s) 247
FatI CATG 1 cut(s) 360
Fnu4HI GCNGC 1 cut(s) 89
FokI GGATG 1 cut(s) 314
Fsp4HI GCNGC 1 cut(s) 89
FspBI CTAG 2 cut(s) 92, 351
GluI GCNGC 1 cut(s) 89
GsuI CTGGAG 1 cut(s) 11
HaeIII GGCC 4 cut(s) 55, 63, 202, 319
Hin1II CATG 1 cut(s) 364
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HphI GGTGA 2 cut(s) 107, 164
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 4 cut(s) 84, 309, 425, 432
Hpy188III TCNNGA 3 cut(s) 212, 361, 418
Hpy8I GTNNAC 1 cut(s) 73
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 1 cut(s) 268
HpyCH4IV ACGT 4 cut(s) 69, 131, 357, 441
HpyCH4V TGCA 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 1 cut(s) 97
HpyF3I CTNAG 6 cut(s) 16, 32, 237, 312, 392, 429
HpySE526I ACGT 4 cut(s) 69, 131, 357, 441
Hsp92II CATG 1 cut(s) 364
Kzo9I GATC 2 cut(s) 420, 425
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 8 cut(s) 37, 41, 155, 182, 216, 333, 379, 389
Lsp1109I GCAGC 1 cut(s) 100
MaeI CTAG 2 cut(s) 92, 351
MaeII ACGT 4 cut(s) 69, 131, 357, 441
MaeIII GTNAC 2 cut(s) 241, 353
MalI GATC 2 cut(s) 422, 427
MboI GATC 2 cut(s) 420, 425
MflI RGATCY 1 cut(s) 420
MseI TTAA 1 cut(s) 438
MspR9I CCNGG 1 cut(s) 170
MvaI CCWGG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 97
NdeII GATC 2 cut(s) 420, 425
NlaIII CATG 1 cut(s) 364
NmeAIII GCCGAG 1 cut(s) 178
NmuCI GTSAC 2 cut(s) 241, 353
PagI TCATGA 1 cut(s) 360
PflMI CCANNNNNTGG 1 cut(s) 175
PkrI GCNGC 1 cut(s) 90
PshAI GACNNNNGTC 1 cut(s) 239
Psp1406I AACGTT 1 cut(s) 441
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PsuI RGATCY 1 cut(s) 420
RsaI GTAC 4 cut(s) 47, 137, 293, 402
RsaNI GTAC 4 cut(s) 46, 136, 292, 401
SaqAI TTAA 1 cut(s) 438
SatI GCNGC 1 cut(s) 89
Sau3AI GATC 2 cut(s) 420, 425
ScrFI CCNGG 1 cut(s) 170
SpeI ACTAGT 1 cut(s) 350
SspMI CTAG 2 cut(s) 92, 351
StyD4I CCNGG 1 cut(s) 168
StyI CCWWGG 2 cut(s) 147, 229
TaiI ACGT 4 cut(s) 72, 134, 360, 444
Tru1I TTAA 1 cut(s) 438
Tru9I TTAA 1 cut(s) 438
TscAI CASTG 2 cut(s) 40, 250
TseFI GTSAC 2 cut(s) 241, 353
TseI GCWGC 1 cut(s) 88
Tsp45I GTSAC 2 cut(s) 241, 353
TspDTI ATGAA 2 cut(s) 17, 274
TspRI CASTG 2 cut(s) 40, 250
Van91I CCANNNNNTGG 1 cut(s) 175
XagI CCTNNNNNAGG 1 cut(s) 395
XspI CTAG 2 cut(s) 92, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.