Rw4G009730

At4g14100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0015595
Physical Location & Seq
Forward (+)
21990523 .. 21992145
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009730.1

Sequence Viewer

Length: 579 bp
ATGAAGCAACTCAGTCGACTTATGCAACTATGCTTAGCAACCTCAACCTGGCTAATCCTAGTGACTTGCTCAAACCTAACCCCAACACCTTCACCATGGCCAGAGCAATTCCACGCGCTTCTGTACATGAACATCACTTCCTCAGCCCATCTCCAGCTCAGTGATGTATGGTACGACTGGCCTAAAGGCCGAAACGTCAACATATTTCAGAAGCAGCTAGGTGAGCTTTTGTATGATGTAGAGTGGAACAACGGCACGTCGTACTACTACACCTTGGGTGAGGGAGGACATTGCCAGGTTATGGCGTTTGAAGTGGGTATTCCTCGGCCTGATTTTCTTGATGGAGCAAACTACCTTGGGACTGAGGTCACTGATGGGTTTCTCTGCAATGTGTGGGAGAAGGTGGATTTCATTTGGTACTATGAGGATGTTCAGACTAAGAGGCCAGTTCGTTGGGATTTCTATGATGGAATAACTAGTCACGTCATGACATTTGAGGTTGGTGCAGTCTTGCCGGATTCAGTTACTCAAGCACCTGCGCACTGTTTCAATCAAAACAGAAATGCTGCTGCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.97

Weight (kDa)

4.82

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08920 FvH4_4g08920 FvH4_4g08920
malus_domestica MD13G1264800.v1.1
prunus_persica Prupe.1G092000_v2.0.a1
pyrus_communis pycom13g23700
rosa_chinensis RchiOBHm_Chr4g0403651
rosa_laevigata RLG00000008962
rosa_multiflora Rmu_co8223106.1_g000001
rosa_roxburghii Rroxscaffold_5G00348250
rosa_rugosa Rorug04G0044800
rosa_samantha Rh4AG119800 Rh4BG113100 Rh4CG128000 Rh4DG112500
rosa_wichuraiana Rw4G009730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 544
Acc16I TGCGCA 1 cut(s) 540
Acc36I ACCTGC 1 cut(s) 544
AccB7I CCANNNNNTGG 1 cut(s) 301
AccI GTMKAC 1 cut(s) 16
AccII CGCG 1 cut(s) 116
AcoI YGGCCR 1 cut(s) 98
AfaI GTAC 4 cut(s) 125, 173, 263, 419
AfiI CCNNNNNNNGG 2 cut(s) 48, 301
AgsI TTSAA 2 cut(s) 311, 550
AhlI ACTAGT 1 cut(s) 476
AjiI CACGTC 2 cut(s) 258, 484
AjnI CCWGG 2 cut(s) 47, 294
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 3 cut(s) 157, 217, 226
AluI AGCT 3 cut(s) 157, 217, 226
AoxI GGCC 5 cut(s) 98, 179, 187, 326, 443
ApeKI GCWGC 4 cut(s) 214, 566, 569, 572
AspLEI GCGC 2 cut(s) 118, 541
AsuHPI GGTGA 3 cut(s) 84, 233, 290
BalI TGGCCA 1 cut(s) 100
BbvCI CCTCAGC 1 cut(s) 142
BbvI GCAGC 4 cut(s) 226, 553, 556, 559
BccI CCATC 4 cut(s) 156, 335, 368, 461
BceAI ACGGC 1 cut(s) 268
BcgI CGANNNNNNTGC 1 cut(s) 30
BciT130I CCWGG 2 cut(s) 49, 296
BcuI ACTAGT 1 cut(s) 476
BfaI CTAG 3 cut(s) 59, 218, 477
BfuAI ACCTGC 1 cut(s) 544
BisI GCNGC 4 cut(s) 215, 567, 570, 573
BlpI GCTNAGC 1 cut(s) 34
BlsI GCNGC 4 cut(s) 216, 568, 571, 574
Bme1390I CCNGG 2 cut(s) 49, 296
BmgBI CACGTC 2 cut(s) 258, 484
BmrFI CCNGG 2 cut(s) 49, 296
BoxI GACNNNNGTC 1 cut(s) 365
BpmI CTGGAG 1 cut(s) 137
Bpu10I CCTNAGC 1 cut(s) 142
Bpu1102I GCTNAGC 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 513
BsaJI CCNNGG 4 cut(s) 95, 273, 323, 355
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 301
Bse1I ACTGG 2 cut(s) 182, 446
Bse3DI GCAATG 2 cut(s) 289, 394
BseBI CCWGG 2 cut(s) 49, 296
BseDI CCNNGG 4 cut(s) 95, 273, 323, 355
BseGI GGATG 1 cut(s) 433
BseLI CCNNNNNNNGG 2 cut(s) 48, 301
BseMI GCAATG 2 cut(s) 289, 394
BseMII CTCAG 4 cut(s) 25, 156, 172, 354
BseNI ACTGG 2 cut(s) 182, 446
BseXI GCAGC 4 cut(s) 226, 553, 556, 559
BsgI GTGCAG 1 cut(s) 525
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 5 cut(s) 100, 181, 189, 328, 445
BsiSI CCGG 1 cut(s) 515
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 2 cut(s) 48, 301
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 5 cut(s) 100, 181, 189, 328, 445
Bsp1407I TGTACA 1 cut(s) 123
Bsp1720I GCTNAGC 1 cut(s) 34
Bsp19I CCATGG 1 cut(s) 95
BspANI GGCC 5 cut(s) 100, 181, 189, 328, 445
BspCNI CTCAG 4 cut(s) 24, 155, 171, 355
BspFNI CGCG 1 cut(s) 116
BspHI TCATGA 1 cut(s) 486
BspMI ACCTGC 1 cut(s) 544
BsrDI GCAATG 2 cut(s) 289, 394
BsrGI TGTACA 1 cut(s) 123
BsrI ACTGG 2 cut(s) 182, 446
BssECI CCNNGG 4 cut(s) 95, 273, 323, 355
BssT1I CCWWGG 3 cut(s) 95, 273, 355
Bst2UI CCWGG 2 cut(s) 49, 296
Bst4CI ACNGT 1 cut(s) 545
BstAUI TGTACA 1 cut(s) 123
BstDEI CTNAG 6 cut(s) 11, 34, 142, 158, 363, 438
BstDSI CCRYGG 1 cut(s) 95
BstF5I GGATG 1 cut(s) 433
BstFNI CGCG 1 cut(s) 116
BstHHI GCGC 2 cut(s) 118, 541
BstMWI GCNNNNNNNGC 2 cut(s) 223, 572
BstNI CCWGG 2 cut(s) 49, 296
BstPAI GACNNNNGTC 1 cut(s) 365
BstSCI CCNGG 2 cut(s) 47, 294
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 4 cut(s) 226, 553, 556, 559
BstXI CCANNNNNNTGG 1 cut(s) 453
BsuRI GGCC 5 cut(s) 100, 181, 189, 328, 445
BtgI CCRYGG 1 cut(s) 95
BtrI CACGTC 2 cut(s) 258, 484
BtsCI GGATG 1 cut(s) 433
BtsIMutI CAGTG 3 cut(s) 166, 369, 541
BveI ACCTGC 1 cut(s) 544
CciI TCATGA 1 cut(s) 486
CfoI GCGC 2 cut(s) 118, 541
Csp6I GTAC 4 cut(s) 124, 172, 262, 418
CviAII CATG 3 cut(s) 96, 127, 487
CviQI GTAC 4 cut(s) 124, 172, 262, 418
DdeI CTNAG 6 cut(s) 11, 34, 142, 158, 363, 438
EaeI YGGCCR 1 cut(s) 98
Eco130I CCWWGG 3 cut(s) 95, 273, 355
EcoRII CCWGG 2 cut(s) 47, 294
EcoT14I CCWWGG 3 cut(s) 95, 273, 355
ErhI CCWWGG 3 cut(s) 95, 273, 355
FaeI CATG 3 cut(s) 99, 130, 490
FaqI GGGAC 1 cut(s) 373
FatI CATG 3 cut(s) 95, 126, 486
FblI GTMKAC 1 cut(s) 16
Fnu4HI GCNGC 4 cut(s) 215, 567, 570, 573
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 4 cut(s) 215, 567, 570, 573
FspBI CTAG 3 cut(s) 59, 218, 477
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 2 cut(s) 117, 540
GluI GCNGC 4 cut(s) 215, 567, 570, 573
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 5 cut(s) 100, 181, 189, 328, 445
HapII CCGG 1 cut(s) 515
HhaI GCGC 2 cut(s) 118, 541
Hin1II CATG 3 cut(s) 99, 130, 490
Hin6I GCGC 2 cut(s) 116, 539
HinP1I GCGC 2 cut(s) 116, 539
HincII GTYRAC 2 cut(s) 17, 199
HindII GTYRAC 2 cut(s) 17, 199
HinfI GANTC 1 cut(s) 518
HpaII CCGG 1 cut(s) 515
HphI GGTGA 3 cut(s) 84, 233, 290
Hpy166II GTNNAC 2 cut(s) 17, 199
Hpy188I TCNGA 2 cut(s) 210, 435
Hpy188III TCNNGA 2 cut(s) 338, 487
Hpy8I GTNNAC 2 cut(s) 17, 199
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 2 cut(s) 99, 394
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4IV ACGT 3 cut(s) 195, 257, 483
HpyCH4V TGCA 4 cut(s) 25, 387, 506, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 223, 572
HpyF3I CTNAG 6 cut(s) 11, 34, 142, 158, 363, 438
HpySE526I ACGT 3 cut(s) 195, 257, 483
Hsp92II CATG 3 cut(s) 99, 130, 490
HspAI GCGC 2 cut(s) 116, 539
LmnI GCTCC 1 cut(s) 344
Lsp1109I GCAGC 4 cut(s) 226, 553, 556, 559
MaeI CTAG 3 cut(s) 59, 218, 477
MaeII ACGT 3 cut(s) 195, 257, 483
MaeIII GTNAC 4 cut(s) 61, 367, 479, 523
MlsI TGGCCA 1 cut(s) 100
MluCI AATT 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 100
MnlI CCTC 9 cut(s) 52, 151, 274, 278, 333, 358, 418, 435, 490
Mox20I TGGCCA 1 cut(s) 100
MscI TGGCCA 1 cut(s) 100
Msp20I TGGCCA 1 cut(s) 100
MspI CCGG 1 cut(s) 515
MspR9I CCNGG 2 cut(s) 49, 296
MvaI CCWGG 2 cut(s) 49, 296
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 223, 572
NcoI CCATGG 1 cut(s) 95
NlaIII CATG 3 cut(s) 99, 130, 490
NmeAIII GCCGAG 1 cut(s) 304
NmuCI GTSAC 3 cut(s) 61, 367, 479
NsbI TGCGCA 1 cut(s) 540
PagI TCATGA 1 cut(s) 486
PaqCI CACCTGC 1 cut(s) 544
PfeI GAWTC 1 cut(s) 518
PflMI CCANNNNNTGG 1 cut(s) 301
PkrI GCNGC 4 cut(s) 216, 568, 571, 574
PshAI GACNNNNGTC 1 cut(s) 365
Psp6I CCWGG 2 cut(s) 47, 294
PspGI CCWGG 2 cut(s) 47, 294
RsaI GTAC 4 cut(s) 125, 173, 263, 419
RsaNI GTAC 4 cut(s) 124, 172, 262, 418
SalI GTCGAC 1 cut(s) 15
SatI GCNGC 4 cut(s) 215, 567, 570, 573
ScrFI CCNGG 2 cut(s) 49, 296
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
SpeI ACTAGT 1 cut(s) 476
Sse9I AATT 1 cut(s) 107
SspMI CTAG 3 cut(s) 59, 218, 477
StyD4I CCNGG 2 cut(s) 47, 294
StyI CCWWGG 3 cut(s) 95, 273, 355
TaaI ACNGT 1 cut(s) 545
TaiI ACGT 3 cut(s) 198, 260, 486
TaqI TCGA 1 cut(s) 16
TasI AATT 1 cut(s) 107
TatI WGTACW 1 cut(s) 123
TfiI GAWTC 1 cut(s) 518
TscAI CASTG 3 cut(s) 166, 376, 548
TseFI GTSAC 3 cut(s) 61, 367, 479
TseI GCWGC 4 cut(s) 214, 566, 569, 572
Tsp45I GTSAC 3 cut(s) 61, 367, 479
TspDTI ATGAA 3 cut(s) 17, 143, 400
TspRI CASTG 3 cut(s) 166, 376, 548
Van91I CCANNNNNTGG 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 16
XspI CTAG 3 cut(s) 59, 218, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.