FvH4_4g11160

Gamma-secretase subunit APH1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012099
Physical Location & Seq
Reverse (-)
14960478 .. 14963118
2641 bp
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UTR
Exon/CDS
Intron
FvH4_4g11160.t2

Sequence Viewer

Length: 600 bp
ATGCTGTCCGGTTTCTGGAGGGCATTTCTGCCCCTCAACTCGTGGTGGCCCTATGCTCTTCTCATCTTCACCTCCGTCCTTTTTCAAGAAGCCCTTCGCATTCTCTTCTGGAAGGTCTATAAGAAGTTGGAAGATACGTTGGATGCATTTGCTGATAGAGTATCTAAGCCACGCCTCTTTCTGACTGATAAGATGCAAATTGCTCTGGCTGGTGGTTTGGGTCACGGCGTGGCACATGCTGTGTTCTTTTGTGTGAGTCTCTTAACGCCAGCTTTTGGTCCAGGAACTTTTTTTGTGGATAGGTGCTCAAAGATACCTTTTTTTCTTATTTCTGCTATCATTGCACTTGCATTTGTTACAATTCACACATTTTCAATGGTCATTGCATTTAATGGATATGCAGAAGGCAGCAAAGTGGACCAGATTGTTGTACCTTTAGTTCATCTTGTTGCAGGGATGGTGACACTAGTTAATTTTGCATCTGGGGGATGTATTATCGGTATTCCTCTCCTCTACTTCATTGCTGTGTTCACCTTATTGCATTGTGGGAAGATGGTGTGGAGAAGGTTAACAGAGAACAGACACAGGCCACCCATGTAG

Protein Analysis

200

Amino Acids

22.29

Weight (kDa)

9.51

Isoelectric Point (pI)

19.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aph-1 PF06105 5 - 236 1.5e-76 Aph-1 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31440 AT2G31440
fragaria_vesca FvH4_4g11160 FvH4_4g11160
malus_domestica MD04G1039500.v1.1
prunus_persica Prupe.1G190300_v2.0.a1 Prupe.1G190300_v2.0.a1 Prupe.1G190300_v2.0.a1
pyrus_communis pycom04g03260
rosa_chinensis RchiOBHm_Chr4g0407291
rosa_laevigata RLG00000008735
rosa_multiflora Rmu_sc0001860.1_g000011
rosa_roxburghii Rroxscaffold_5G00351830
rosa_rugosa Rorug04G0078500
rosa_samantha Rh4AG143200 Rh4BG140700 Rh4CG149600 Rh4DG137100
rosa_wichuraiana Rw4G011640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 275
AdeI CACNNNGTG 1 cut(s) 229
AfaI GTAC 1 cut(s) 432
AfiI CCNNNNNNNGG 2 cut(s) 15, 275
AgsI TTSAA 2 cut(s) 86, 375
AhlI ACTAGT 1 cut(s) 466
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 1 cut(s) 272
AluI AGCT 1 cut(s) 272
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 1 cut(s) 263
AoxI GGCC 2 cut(s) 47, 587
ApeKI GCWGC 1 cut(s) 408
Asp700I GAANNNNTTC 1 cut(s) 93
AspS9I GGNCC 3 cut(s) 48, 278, 418
AsuHPI GGTGA 3 cut(s) 61, 472, 523
AvaII GGWCC 2 cut(s) 278, 418
BauI CACGAG 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 1 cut(s) 420
BccI CCATC 2 cut(s) 451, 547
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 263
BcuI ACTAGT 1 cut(s) 466
BfaI CTAG 1 cut(s) 467
BisI GCNGC 1 cut(s) 409
BlsI GCNGC 1 cut(s) 410
Bme1390I CCNGG 1 cut(s) 282
Bme18I GGWCC 2 cut(s) 278, 418
BmgT120I GGNCC 3 cut(s) 48, 278, 418
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 133, 183, 488
BpmI CTGGAG 1 cut(s) 37
BsaWI WCCGGW 1 cut(s) 8
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 275
Bse3DI GCAATG 3 cut(s) 339, 381, 519
BseBI CCWGG 1 cut(s) 282
BseGI GGATG 3 cut(s) 148, 462, 494
BseLI CCNNNNNNNGG 2 cut(s) 15, 275
BseMI GCAATG 3 cut(s) 339, 381, 519
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 1 cut(s) 420
BshFI GGCC 2 cut(s) 49, 589
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 1 cut(s) 9
BslI CCNNNNNNNGG 2 cut(s) 15, 275
BsmAI GTCTC 1 cut(s) 263
BsmI GAATGC 1 cut(s) 99
BsnI GGCC 2 cut(s) 49, 589
Bsp1286I GDGCHC 1 cut(s) 308
BspANI GGCC 2 cut(s) 49, 589
BspQI GCTCTTC 1 cut(s) 63
BsrDI GCAATG 3 cut(s) 339, 381, 519
BssSI CACGAG 1 cut(s) 40
Bst2BI CACGAG 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 282
Bst6I CTCTTC 2 cut(s) 63, 110
BstC8I GCNNGC 1 cut(s) 270
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 148, 462, 494
BstMAI GTCTC 1 cut(s) 263
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNI CCWGG 1 cut(s) 282
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 420
BsuRI GGCC 2 cut(s) 49, 589
BtsCI GGATG 3 cut(s) 148, 462, 494
Cac8I GCNNGC 1 cut(s) 270
Cfr13I GGNCC 3 cut(s) 48, 278, 418
Csp6I GTAC 1 cut(s) 431
CviAII CATG 2 cut(s) 236, 595
CviJI RGCY 6 cut(s) 49, 92, 169, 209, 272, 589
CviKI_1 RGCY 6 cut(s) 49, 92, 169, 209, 272, 589
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 1 cut(s) 165
DraIII CACNNNGTG 1 cut(s) 229
Eam1104I CTCTTC 2 cut(s) 63, 110
EarI CTCTTC 2 cut(s) 63, 110
Eco47I GGWCC 2 cut(s) 278, 418
EcoRII CCWGG 1 cut(s) 280
EcoT22I ATGCAT 1 cut(s) 148
FaeI CATG 2 cut(s) 239, 598
FaiI YATR 5 cut(s) 54, 120, 237, 399, 596
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FatI CATG 2 cut(s) 235, 594
Fnu4HI GCNGC 1 cut(s) 409
FokI GGATG 3 cut(s) 155, 469, 501
Fsp4HI GCNGC 1 cut(s) 409
FspBI CTAG 1 cut(s) 467
GluI GCNGC 1 cut(s) 409
GsuI CTGGAG 1 cut(s) 37
HaeIII GGCC 2 cut(s) 49, 589
HapII CCGG 1 cut(s) 9
Hin1II CATG 2 cut(s) 239, 598
HincII GTYRAC 1 cut(s) 570
HindII GTYRAC 1 cut(s) 570
HinfI GANTC 1 cut(s) 256
HpaI GTTAAC 1 cut(s) 570
HpaII CCGG 1 cut(s) 9
HphI GGTGA 3 cut(s) 61, 472, 523
Hpy166II GTNNAC 3 cut(s) 418, 531, 570
Hpy188I TCNGA 1 cut(s) 183
Hpy188III TCNNGA 3 cut(s) 16, 86, 109
Hpy8I GTNNAC 3 cut(s) 418, 531, 570
HpyAV CCTTC 4 cut(s) 104, 106, 398, 558
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 9 cut(s) 146, 196, 344, 350, 386, 401, 452, 479, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 1 cut(s) 165
HpySE526I ACGT 1 cut(s) 137
Hsp92II CATG 2 cut(s) 239, 598
KspAI GTTAAC 1 cut(s) 570
LguI GCTCTTC 1 cut(s) 63
Lsp1109I GCAGC 1 cut(s) 420
LweI GCATC 3 cut(s) 133, 183, 488
MaeI CTAG 1 cut(s) 467
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 3 cut(s) 221, 355, 460
MboII GAAGA 5 cut(s) 50, 58, 97, 143, 562
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 3 cut(s) 198, 360, 472
MlyI GAGTC 1 cut(s) 265
MmeI TCCRAC 2 cut(s) 108, 120
MnlI CCTC 6 cut(s) 12, 44, 82, 185, 516, 521
Mph1103I ATGCAT 1 cut(s) 148
MroXI GAANNNNTTC 1 cut(s) 93
MseI TTAA 4 cut(s) 263, 390, 471, 569
MslI CAYNNNNRTG 1 cut(s) 524
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 99
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 341
NlaIII CATG 2 cut(s) 239, 598
NmuCI GTSAC 2 cut(s) 221, 460
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 1 cut(s) 239
PciSI GCTCTTC 1 cut(s) 63
PctI GAATGC 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 275
PfoI TCCNGGA 1 cut(s) 280
PkrI GCNGC 1 cut(s) 410
PleI GAGTC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 280
PspGI CCWGG 1 cut(s) 280
PspPI GGNCC 3 cut(s) 48, 278, 418
PsrI GAACNNNNNNTAC 2 cut(s) 423, 455
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
RseI CAYNNNNRTG 1 cut(s) 524
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 4 cut(s) 263, 390, 471, 569
SatI GCNGC 1 cut(s) 409
Sau96I GGNCC 3 cut(s) 48, 278, 418
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 1 cut(s) 308
SetI ASST 9 cut(s) 74, 117, 140, 274, 305, 319, 436, 536, 569
SfaNI GCATC 3 cut(s) 133, 183, 488
SinI GGWCC 2 cut(s) 278, 418
SmiMI CAYNNNNRTG 1 cut(s) 524
SpeI ACTAGT 1 cut(s) 466
Sse9I AATT 3 cut(s) 198, 360, 472
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 1 cut(s) 280
TaiI ACGT 1 cut(s) 140
TasI AATT 3 cut(s) 198, 360, 472
Tru1I TTAA 4 cut(s) 263, 390, 471, 569
Tru9I TTAA 4 cut(s) 263, 390, 471, 569
TseFI GTSAC 2 cut(s) 221, 460
TseI GCWGC 1 cut(s) 408
Tsp45I GTSAC 2 cut(s) 221, 460
TspDTI ATGAA 2 cut(s) 431, 508
TspGWI ACGGA 1 cut(s) 64
Van91I CCANNNNNTGG 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 278, 418
XceI RCATGY 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 93
XspI CTAG 1 cut(s) 467
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.