MD04G1039500.v1.1

Gamma-secretase subunit APH1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012099
Physical Location & Seq
Forward (+)
4324009 .. 4328222
4214 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1039500.v1.1.491

Sequence Viewer

Length: 750 bp
ATGACGATAGCAGCAGGGATCGGCTACGCCTTGCTAGCGCTAGGCCCCTCCCTCTCCCTCTTCGTCTCTGTCATCTCCAAGAAACCCTTTCTGGTTCTTACCGTCCTTTCAAGTACATTGTTATGGCTTATAAGTCTCATCGTGTTGTCGGGATTGTGGAGGGCATTCCTACCTCTGAAATCATCGGCATGGTGGCCGTATGGCATTCTCATATTCACATCGGTTGTGTTTCAAGAAGGGCTTCGGGTTCTCTTCTGGAAGGTCTACAAGAAGTTGGAAGATACATTGGATGCTTTTGCTGATAGAGTCTCTAAACCACGGCTATATTTAACTGATAAGATGCAAATCGCTCTGGCTGGAGGTCTGGGTCATGGTGTGGCACATGCTGTGTTTTTCTGTCTCAGCCTATTAACACCAGCATTTGGTCCAGCAACCTTCTTCGTTGACAGGTGCTCGAAGATACCATTTTTTCTCCTCTCTGCACTCATCGCCCTTGCGTTTGTAACAATTCATACTTTCTCCATGGTCATTGCATTCAATGGATATGCAGAAGGGAACAAGGTGGACCAGATTTTTGTTCCTGTAGTTCATCTTGTAGCAGGCCTGGTGACACTTGTTAATTTTGCATCTGGGGGTTGTATCATCGGTATTCCGCTCCTTTTCTTCATCGCAATCCTGACGTTATTGCATTGTGGGAAGATGGTGTGGAGGAGGTTGACAGAGAACCGACACAGACAGGGTAGCATGTAG

Protein Analysis

250

Amino Acids

27.38

Weight (kDa)

9.73

Isoelectric Point (pI)

24.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aph-1 PF06105 5 - 238 5.4e-74 Aph-1 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012099)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31440 AT2G31440
fragaria_vesca FvH4_4g11160 FvH4_4g11160
malus_domestica MD04G1039500.v1.1
prunus_persica Prupe.1G190300_v2.0.a1 Prupe.1G190300_v2.0.a1 Prupe.1G190300_v2.0.a1
pyrus_communis pycom04g03260
rosa_chinensis RchiOBHm_Chr4g0407291
rosa_laevigata RLG00000008735
rosa_multiflora Rmu_sc0001860.1_g000011
rosa_roxburghii Rroxscaffold_5G00351830
rosa_rugosa Rorug04G0078500
rosa_samantha Rh4AG143200 Rh4BG140700 Rh4CG149600 Rh4DG137100
rosa_wichuraiana Rw4G011640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AccB7I CCANNNNNTGG 1 cut(s) 422
AccBSI CCGCTC 1 cut(s) 655
AccI GTMKAC 1 cut(s) 264
AciI CCGC 1 cut(s) 653
AclWI GGATC 1 cut(s) 26
AcoI YGGCCR 1 cut(s) 194
AfaI GTAC 1 cut(s) 115
AfeI AGCGCT 1 cut(s) 39
AfiI CCNNNNNNNGG 1 cut(s) 422
AgsI TTSAA 3 cut(s) 111, 233, 538
AjnI CCWGG 1 cut(s) 603
Alw21I GWGCWC 1 cut(s) 455
Alw26I GTCTC 4 cut(s) 70, 140, 313, 404
AlwI GGATC 1 cut(s) 26
Aor51HI AGCGCT 1 cut(s) 39
AoxI GGCC 3 cut(s) 43, 194, 601
ApeKI GCWGC 1 cut(s) 11
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
Asp700I GAANNNNTTC 1 cut(s) 240
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 3 cut(s) 44, 425, 565
AsuHPI GGTGA 1 cut(s) 619
AsuNHI GCTAGC 1 cut(s) 34
AvaII GGWCC 2 cut(s) 425, 565
Bbv12I GWGCWC 1 cut(s) 455
BbvI GCAGC 1 cut(s) 23
BccI CCATC 1 cut(s) 694
BceAI ACGGC 2 cut(s) 181, 335
BciT130I CCWGG 1 cut(s) 605
BcoDI GTCTC 4 cut(s) 70, 140, 313, 404
BfaI CTAG 2 cut(s) 35, 41
BfmI CTRYAG 1 cut(s) 582
BfoI RGCGCY 1 cut(s) 41
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 605
Bme18I GGWCC 2 cut(s) 425, 565
BmgT120I GGNCC 3 cut(s) 44, 425, 565
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 1 cut(s) 605
BmsI GCATC 3 cut(s) 280, 330, 635
BmtI GCTAGC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 378
BsaBI GATNNNNATC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 317, 522
Bsc4I CCNNNNNNNGG 1 cut(s) 422
Bse3DI GCAATG 1 cut(s) 528
Bse8I GATNNNNATC 1 cut(s) 344
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 317, 522
BseGI GGATG 1 cut(s) 295
BseJI GATNNNNATC 1 cut(s) 344
BseLI CCNNNNNNNGG 1 cut(s) 422
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 1 cut(s) 415
BseRI GAGGAG 2 cut(s) 464, 724
BseXI GCAGC 1 cut(s) 23
BsgI GTGCAG 1 cut(s) 465
BshFI GGCC 3 cut(s) 45, 196, 603
BsiHKAI GWGCWC 1 cut(s) 455
BslI CCNNNNNNNGG 1 cut(s) 422
BsmAI GTCTC 4 cut(s) 70, 140, 313, 404
BsmBI CGTCTC 1 cut(s) 70
BsmI GAATGC 3 cut(s) 164, 204, 533
BsnI GGCC 3 cut(s) 45, 196, 603
Bsp1286I GDGCHC 1 cut(s) 455
Bsp143I GATC 1 cut(s) 18
Bsp19I CCATGG 1 cut(s) 522
BspACI CCGC 1 cut(s) 653
BspANI GGCC 3 cut(s) 45, 196, 603
BspCNI CTCAG 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 46
BspOI GCTAGC 1 cut(s) 38
BspPI GGATC 1 cut(s) 26
BsrBI CCGCTC 1 cut(s) 655
BsrDI GCAATG 1 cut(s) 528
BssECI CCNNGG 2 cut(s) 317, 522
BssMI GATC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 2 cut(s) 65, 257
BstC8I GCNNGC 2 cut(s) 36, 601
BstDEI CTNAG 1 cut(s) 401
BstDSI CCRYGG 2 cut(s) 317, 522
BstF5I GGATG 1 cut(s) 295
BstH2I RGCGCY 1 cut(s) 41
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 1 cut(s) 21
BstMAI GTCTC 4 cut(s) 70, 140, 313, 404
BstMBI GATC 1 cut(s) 18
BstMWI GCNNNNNNNGC 2 cut(s) 35, 488
BstNI CCWGG 1 cut(s) 605
BstNSI RCATGY 2 cut(s) 386, 748
BstSCI CCNGG 1 cut(s) 603
BstSFI CTRYAG 1 cut(s) 582
BstV1I GCAGC 1 cut(s) 23
BsuRI GGCC 3 cut(s) 45, 196, 603
BtgI CCRYGG 2 cut(s) 317, 522
BtgZI GCGATG 2 cut(s) 472, 652
BtsCI GGATG 1 cut(s) 295
Cac8I GCNNGC 2 cut(s) 36, 601
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 44, 425, 565
Csp6I GTAC 1 cut(s) 114
CviAII CATG 5 cut(s) 189, 371, 383, 523, 745
CviJI RGCY 9 cut(s) 24, 45, 127, 196, 241, 322, 356, 405, 603
CviKI_1 RGCY 9 cut(s) 24, 45, 127, 196, 241, 322, 356, 405, 603
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 1 cut(s) 401
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
EaeI YGGCCR 1 cut(s) 194
Eam1104I CTCTTC 2 cut(s) 65, 257
EarI CTCTTC 2 cut(s) 65, 257
Eco130I CCWWGG 1 cut(s) 522
Eco147I AGGCCT 1 cut(s) 603
Eco47I GGWCC 2 cut(s) 425, 565
Eco47III AGCGCT 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 522
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 5 cut(s) 192, 374, 386, 526, 748
FalI AAGNNNNNCTT 4 cut(s) 71, 103, 225, 257
FatI CATG 5 cut(s) 188, 370, 382, 522, 744
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 1 cut(s) 302
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 2 cut(s) 35, 41
GlaI GCGC 1 cut(s) 39
GluI GCNGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 378
HaeII RGCGCY 1 cut(s) 41
HaeIII GGCC 3 cut(s) 45, 196, 603
HhaI GCGC 1 cut(s) 40
Hin1II CATG 5 cut(s) 192, 374, 386, 526, 748
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 445, 717
HindII GTYRAC 2 cut(s) 445, 717
HinfI GANTC 1 cut(s) 306
HphI GGTGA 1 cut(s) 619
Hpy166II GTNNAC 4 cut(s) 265, 445, 565, 717
Hpy188I TCNGA 1 cut(s) 177
Hpy188III TCNNGA 4 cut(s) 150, 233, 256, 676
Hpy8I GTNNAC 4 cut(s) 265, 445, 565, 717
HpyAV CCTTC 4 cut(s) 230, 253, 445, 545
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 1 cut(s) 680
HpyCH4V TGCA 6 cut(s) 343, 482, 533, 548, 626, 688
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 488
HpyF3I CTNAG 1 cut(s) 401
HpySE526I ACGT 1 cut(s) 680
Hsp92II CATG 5 cut(s) 192, 374, 386, 526, 748
HspAI GCGC 1 cut(s) 38
Kzo9I GATC 1 cut(s) 18
LmnI GCTCC 1 cut(s) 660
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 3 cut(s) 280, 330, 635
MaeI CTAG 2 cut(s) 35, 41
MaeII ACGT 1 cut(s) 680
MaeIII GTNAC 2 cut(s) 502, 607
MalI GATC 1 cut(s) 20
MbiI CCGCTC 1 cut(s) 655
MboI GATC 1 cut(s) 18
MboII GAAGA 7 cut(s) 52, 244, 290, 430, 469, 655, 709
MhlI GDGCHC 1 cut(s) 455
MluCI AATT 2 cut(s) 507, 619
MlyI GAGTC 1 cut(s) 315
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 9 cut(s) 58, 62, 68, 153, 183, 353, 485, 702, 705
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 3 cut(s) 329, 410, 618
MslI CAYNNNNRTG 2 cut(s) 121, 187
MspR9I CCNGG 1 cut(s) 605
Mva1269I GAATGC 3 cut(s) 164, 204, 533
MvaI CCWGG 1 cut(s) 605
MwoI GCNNNNNNNGC 2 cut(s) 35, 488
NcoI CCATGG 1 cut(s) 522
NdeII GATC 1 cut(s) 18
NheI GCTAGC 1 cut(s) 34
NlaIII CATG 5 cut(s) 192, 374, 386, 526, 748
NlaIV GGNNCC 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 607
NspI RCATGY 2 cut(s) 386, 748
PceI AGGCCT 1 cut(s) 603
PctI GAATGC 3 cut(s) 164, 204, 533
PdmI GAANNNNTTC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 422
PkrI GCNGC 1 cut(s) 13
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
PsiI TTATAA 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 3 cut(s) 44, 425, 565
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
RseI CAYNNNNRTG 2 cut(s) 121, 187
SaqAI TTAA 3 cut(s) 329, 410, 618
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 1 cut(s) 18
Sau96I GGNCC 3 cut(s) 44, 425, 565
SchI GAGTC 1 cut(s) 315
ScrFI CCNGG 1 cut(s) 605
SduI GDGCHC 1 cut(s) 455
SetI ASST 8 cut(s) 175, 264, 364, 437, 452, 564, 683, 716
SfaNI GCATC 3 cut(s) 280, 330, 635
SfcI CTRYAG 1 cut(s) 582
SinI GGWCC 2 cut(s) 425, 565
SmiMI CAYNNNNRTG 2 cut(s) 121, 187
Sse9I AATT 2 cut(s) 507, 619
SseBI AGGCCT 1 cut(s) 603
SsiI CCGC 1 cut(s) 653
SspMI CTAG 2 cut(s) 35, 41
StuI AGGCCT 1 cut(s) 603
StyD4I CCNGG 1 cut(s) 603
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 1 cut(s) 683
TaqI TCGA 1 cut(s) 455
TasI AATT 2 cut(s) 507, 619
TatI WGTACW 1 cut(s) 113
Tru1I TTAA 3 cut(s) 329, 410, 618
Tru9I TTAA 3 cut(s) 329, 410, 618
TseFI GTSAC 1 cut(s) 607
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 3 cut(s) 500, 578, 655
Van91I CCANNNNNTGG 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 425, 565
XceI RCATGY 2 cut(s) 386, 748
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 2 cut(s) 35, 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.