FvH4_4g12630

Regulator of nonsense transcripts

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007975
Physical Location & Seq
Forward (+)
16292604 .. 16303583
10980 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12630.t2

Sequence Viewer

Length: 3561 bp
ATGTCAGTGAATACGAATAGTGAGATGGATCACAATGAGGAGGAATCCGGTGGTGGCGCCGGAGCTGAGCCTCATGGGAAGCAAGATGATGAGGAAGCTGTTGCTCGGCTTGAGGAAATGAAGAAATCAATCGAGTCGAAGATGGCTCTTCGTCAAAGCAATTTGAACCCTGAGAGGCCTGACTCGGGATTTCTGAGGACATTGGATTCCAGTATTAAACGCAACACAGCAGTTATTAAAAAACTAAAGCAGATAAATGAGGAGCAGCGAGAAGGGCTGATGGACGACTTGAGGAGTGTCAATCTTAGCAAATTTGTTAGTGAAGCTGTTACTGCGATTTGTGATGCCAAACTTAGATCGTCCGACATACAAGCAGCAGTTCAAATCTGCTCTCTGCTTCATCAAAGATACAAAGATTTCTCCCCAACCCTTGTTCAAGGTCTTTTGAAGGTGTTCTTTCCTGGAAAGTCTGGGGATGACCCAGATGCAGATAGGAGCCTGAGGGCCATGAAGAAGCGTAGCACTTTGAAACTCCTTTTGGAACTTTTCTTTGTTGGAGTTATAGAAGACGGTGGTATTTTTGTGAATGTTATCAAAGACCTTACCAGTGGAGATCACTTAAAGGACCGGGAAACTACTCAGACAAATTTGACTCTTCTTGCTAGTTTTGCTCGGCAGGGAAGAATGTTTCTTGGTCTTCCACTCTCTGGGCCAGAAATATATGAAGAGTTTTTTAAGGGCCTTAATATCACACCGGATCAAAAGAAGTTCTTTAAGAAGGCATTTCAAACTTATTATGAAGCTGCAGCAGAACTTCTTCAATCTGAGCATAATTCACTTCGCCAAATGGAGCATGAAAATGCAAGAATCATTAACGCTAAAGGAGAGCTCAGTGATGATAGTGCTTCTTCATATGAAAAGTTGCGGAAATCTTATGACCATTTGTACCGCAATGTCTCAACTTTAGCAGAAGCACTTGATATGCAGCCTCCAGTAATGCCAGAGGATGGTCACACAACTAGGGTTACTAGCGGAGAGGATGCTTCATCTCCAGCTGCTGGAAAGGATTCTTCTGCACTTGAAGCCATATGGGACGATGAAGATACTAGGGCTTTCTATGAATGTTTGCCAGATCTTAGAGCATTTGTTCCAGCTGTGTTACTTGGAGAAGCAGAGTCAAAAGTCAATGAGCAATCTGCAAAGACCCAAGAGCAACCAACTGCTACAGAAGAGGCTGCAGAACCTTCTGCAGAAGTTGGTGCTTTACAGGAAGGAAAAATCAGAGAGAAAGGAAAGGATAAGGAAGAAAAAGAAAAAGAAAAAGACAAGAGTAAAGATGCTGACAAAGAGAAAGGTGACAGGAAGGGAGAAAATGAGAAGGAGAAACTAAAAAGTATTGAAGGCACGAATTTGGACGCTTTACTACAAAGGCTCCCAGGTTGTGTGAGCCGTGATCTCATCGACCAATTAACTGTGGAGTTTTGTTATCTAAATTCTAAAGCAAATCGGAAAAAGCTTGTGAGGGCAGTGTTTAATGTCCCCAGGACATCATTGGAGCTACTGCCGTATTACTCACGCATGGTCGCCACTCTGTCAACTTGTATGAAGGATGTATCATCCATGCTCCTGGCTATGTTGGAGGAGGAGTTCAATTTCTTAATTAATAAGAAGGATCAAATGAATATTGAAACAAAGATTAGGAATATTAGATTTATTGGAGAGCTTTGCAAGTTCAGAATTGCGCCAGCTGGCCTTGTGTTTAGTTGTTTGAAGGCTTGTCTGGATGATTTCTCTCATCATAATATTGATGTTGCTTGCAATCTTCTTGAGACCTGTGGGCGTTTTCTGTATCGGTCTCCTGAAACTACTGTGAGGATGGCTAACATGTTGGAAATATTAATGCGCTTAAAGAATGTAAAAAATTTGGATCCTCGGCACAGCACTCTTGTTGAAAATGCTTACTACCTGTGCAAACCGCCTGAGAGATCTGCACGAGTTGCTAAAGTCCGTCCTCCATTGCATCAGTATATAAGAAAGTTGCTATTCTCAGATCTGGATAAATCCACCCTTGAGCATGTGCTGAGGCAACTGCGTAAACTACCATGGGGTGAATGTGAGCCATATCTTTTGAAATGCTTTTTGAAGGTTCACAAAGGGAAGTATGGTCAGATCCATTTGATTGCTTCTCTCACTGCTGGCCTGAGTCGCTATCATGATGAATTTGCTGTTTCTGTTGTTGACGAGGTTTTAGAGGAGATCAGACTTGGCCTAGAGTTAAATGAGTATGGAATGCAGCAAAGACGAATTGCCCATATGCGATTCCTAGGAGAGCTCTACAACTATGAACATGTTGATTCTTCTGTTATATTTGAGACTCTATACTTGATTCTCATATTTGGCCATGGCACAACAGAGCAAGACGCACTAGATCCACCAGAAGATTGTTTCCGCATGAGGATGGTTATTACTCTTCTTGAAACTTGTGGACATTACTTTGACCGAGGCTCTTCTAAGAGGAAACTTGATCGCTTCTTGATTCACTTCCAGAGATATATACTAAGCAAAGGTGTACTACCACTAGATGTTGAGTTTGACTTACAGGACTTATTTGCAGAATTGCGTCCCAACATGACTCGATACTCATCCCTTGAAGAAGTTAATGCGGCTCTAGTAGAACTTGAGGAACATGAGCGTACTGTCTCTACTGACAAGGCAAATAATGAGAAGCACTCGGACACTGAAAAGTCTTCAAGGAGAACTACCCCCAATAAAACGACAGTGAATGGACAGAGTGTTGTGAATGGTACAGAGGAAAATGGTGTGGTCCATGAAGACCATAGAGATAGTGACAGTGATTCAGGAAGTGGCACTGTTGATCCAGACAGGCATGAAGAAGAATTGGATGAAGAGAATCATGATGATGGATCAGAGAGTGAGGACGACGATGATGATGGGGGTGGACCTGCTTCCGATGAGGACGATGAAGTCCATGTGAGACAGAAGGTTGCAGAGGTAGATCCTCAGGAAGAAGCAGATTTTGAGCTGGATCTCAAGGCTGTAATGCAGGAAAGCATGGAACAACGAAGGCAAGAGCTGCGAGGTCGGCCTACTTTGAATATGATGATACCGATGAACTTGTTTGAGGGGTCCATCAAGGATCATCATGGACGTGTCGGAGGGGACAGTGGTGATGATGGGGATGAAGAGTCTGGAGGAAGCAAGGAGGTCCAAGTCAAAGTTCTTGTGAAGCGCGGTAACAAGCAACAGACAAAGCAGATGTCCATCCCTCGGGATTGCTCACTTGTACAGAGCACGAAACAGAAAGAAGCAGCAGAGCTTGAAGAGAAACAAGACATCAAGAGGCTGGTCTTGGAATATAATGATAGAGAAGAGGAGGAACTTAATGGATTAGGGAACCAAACTTTGAACTATGCGCAGAGTGGGGGCAATAGATTTGGCGGTCGTGGCGGCACCTGGGAAGGAACTAGTGGCAGAGGTGGACCACGTCGATATCATAGTAGCTATTCTGGAGCCGGAGTTTATTACAGCAGGAGAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0031503 GO:0031974 GO:0031981 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034655 GO:0035145 GO:0035690 GO:0035770 GO:0036464 GO:0042162 GO:0042221 GO:0042493 GO:0042742 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046677 GO:0046700 GO:0046907 GO:0048519 GO:0048571 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071426 GO:0071427 GO:0071446 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
MIF4G Upf2

Protein Analysis

1187

Amino Acids

134.08

Weight (kDa)

5.27

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF4G PF02854 73 - 263 6.6e-08 MIF4G domain
MIF4G PF02854 485 - 671 8.9e-41 MIF4G domain
MIF4G PF02854 687 - 882 1.8e-43 MIF4G domain
Upf2 PF04050 1003 - 1136 7e-34 Up-frameshift suppressor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2984
Acc16I TGCGCA 1 cut(s) 3435
Acc36I ACCTGC 1 cut(s) 2971
AccB1I GGYRCC 2 cut(s) 56, 3470
AccB7I CCANNNNNTGG 3 cut(s) 707, 1007, 1058
AccII CGCG 1 cut(s) 3252
AciI CCGC 9 cut(s) 925, 949, 1032, 1976, 2449, 2663, 3252, 3459, 3468
AcoI YGGCCR 1 cut(s) 2398
AcsI RAATTY 6 cut(s) 311, 646, 1408, 1492, 1921, 2219
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 5 cut(s) 947, 2571, 2695, 2806, 3306
AfiI CCNNNNNNNGG 5 cut(s) 185, 707, 1007, 1058, 3288
AflIII ACRYGT 3 cut(s) 1884, 2347, 3169
AhlI ACTAGT 1 cut(s) 3485
AjiI CACGTC 2 cut(s) 3170, 3506
AjnI CCWGG 5 cut(s) 460, 1435, 1541, 1626, 3473
AjuI GAANNNNNNNTTGG 4 cut(s) 521, 553, 3222, 3254
AloI GAACNNNNNNTCC 2 cut(s) 1631, 1663
Alw21I GWGCWC 3 cut(s) 891, 2334, 3314
Alw26I GTCTC 6 cut(s) 961, 1823, 1860, 2366, 2704, 2989
AlwNI CAGNNNCTG 1 cut(s) 1058
Ama87I CYCGRG 2 cut(s) 184, 3288
AoxI GGCC 9 cut(s) 176, 504, 710, 739, 1750, 2197, 2266, 2398, 3104
ApoI RAATTY 6 cut(s) 311, 646, 1408, 1492, 1921, 2219
ArsI GACNNNNNNTTYG 2 cut(s) 3263, 3295
AseI ATTAAT 2 cut(s) 1662, 1898
Asp700I GAANNNNTTC 3 cut(s) 452, 816, 1066
AspA2I CCTAGG 1 cut(s) 2323
AspLEI GCGC 5 cut(s) 59, 1744, 1905, 3252, 3436
AspS9I GGNCC 9 cut(s) 504, 625, 710, 739, 2824, 2960, 3147, 3226, 3500
AsuC2I CCSGG 1 cut(s) 629
AsuHPI GGTGA 3 cut(s) 1367, 2120, 3200
AvaI CYCGRG 2 cut(s) 184, 3288
AvaII GGWCC 6 cut(s) 625, 2824, 2960, 3147, 3226, 3500
AvrII CCTAGG 1 cut(s) 2323
AxyI CCTNAGG 2 cut(s) 500, 3021
BalI TGGCCA 1 cut(s) 2400
BamHI GGATCC 1 cut(s) 1927
BanI GGYRCC 2 cut(s) 56, 3470
BanII GRGCYC 2 cut(s) 891, 2334
BauI CACGAG 1 cut(s) 1992
BbsI GAAGAC 4 cut(s) 573, 689, 2739, 2838
Bbv12I GWGCWC 3 cut(s) 891, 2334, 3314
BbvCI CCTCAGC 1 cut(s) 2081
BceAI ACGGC 2 cut(s) 1434, 1549
BciT130I CCWGG 5 cut(s) 462, 1437, 1543, 1628, 3475
BcnI CCSGG 1 cut(s) 629
BcoDI GTCTC 6 cut(s) 961, 1823, 1860, 2366, 2704, 2989
BcuI ACTAGT 1 cut(s) 3485
BfmI CTRYAG 4 cut(s) 804, 1224, 1236, 1248
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 2971
BglII AGATCT 3 cut(s) 1132, 1985, 2050
BlnI CCTAGG 1 cut(s) 2323
BlpI GCTNAGC 1 cut(s) 66
Bme1390I CCNGG 6 cut(s) 462, 629, 1437, 1543, 1628, 3475
Bme18I GGWCC 6 cut(s) 625, 2824, 2960, 3147, 3226, 3500
BmeT110I CYCGRG 2 cut(s) 184, 3288
BmgBI CACGTC 2 cut(s) 3170, 3506
BmgT120I GGNCC 9 cut(s) 504, 625, 710, 739, 2824, 2960, 3147, 3226, 3500
BmiI GGNNCC 8 cut(s) 58, 497, 1433, 1929, 3148, 3416, 3472, 3532
BmrFI CCNGG 6 cut(s) 462, 629, 1437, 1543, 1628, 3475
BmsI GCATC 5 cut(s) 334, 475, 1030, 1327, 2029
BpiI GAAGAC 4 cut(s) 573, 689, 2739, 2838
BplI GAGNNNNNCTC 2 cut(s) 2714, 2746
BpmI CTGGAG 4 cut(s) 975, 1035, 3231, 3549
Bpu10I CCTNAGC 1 cut(s) 2081
Bpu1102I GCTNAGC 1 cut(s) 66
BpuEI CTTGAG 6 cut(s) 131, 310, 1847, 2090, 2699, 3035
BpuMI CCSGG 1 cut(s) 629
BsaBI GATNNNNATC 2 cut(s) 2641, 3281
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 2 cut(s) 1823, 1860
BsaJI CCNNGG 9 cut(s) 1435, 1541, 1931, 2102, 2323, 2401, 2500, 3287, 3474
BsaWI WCCGGW 2 cut(s) 47, 754
BsaXI ACNNNNNCTCC 6 cut(s) 1416, 1446, 1631, 1661, 3522, 3552
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 707, 1007, 1058, 3288
Bse1I ACTGG 3 cut(s) 210, 606, 992
Bse21I CCTNAGG 2 cut(s) 500, 3021
Bse3DI GCAATG 2 cut(s) 958, 2015
Bse8I GATNNNNATC 2 cut(s) 2641, 3281
BseBI CCWGG 5 cut(s) 462, 1437, 1543, 1628, 3475
BseDI CCNNGG 9 cut(s) 1435, 1541, 1931, 2102, 2323, 2401, 2500, 3287, 3474
BseJI GATNNNNATC 2 cut(s) 2641, 3281
BseLI CCNNNNNNNGG 5 cut(s) 185, 707, 1007, 1058, 3288
BseMI GCAATG 2 cut(s) 958, 2015
BseNI ACTGG 3 cut(s) 210, 606, 992
BseRI GAGGAG 7 cut(s) 53, 275, 307, 1655, 1658, 2267, 3407
BsgI GTGCAG 2 cut(s) 1059, 1974
Bsh1236I CGCG 1 cut(s) 3252
Bsh1285I CGRYCG 1 cut(s) 3463
BshFI GGCC 9 cut(s) 178, 506, 712, 741, 1752, 2199, 2268, 2400, 3106
BshNI GGYRCC 2 cut(s) 56, 3470
BsiEI CGRYCG 1 cut(s) 3463
BsiHKAI GWGCWC 3 cut(s) 891, 2334, 3314
BsiHKCI CYCGRG 2 cut(s) 184, 3288
BsiSI CCGG 5 cut(s) 48, 60, 628, 755, 3534
BslFI GGGAC 4 cut(s) 1106, 1523, 2607, 3194
BslI CCNNNNNNNGG 5 cut(s) 185, 707, 1007, 1058, 3288
BsmAI GTCTC 6 cut(s) 961, 1823, 1860, 2366, 2704, 2989
BsmFI GGGAC 4 cut(s) 1106, 1523, 2607, 3194
BsmI GAATGC 1 cut(s) 2295
BsnI GGCC 9 cut(s) 178, 506, 712, 741, 1752, 2199, 2268, 2400, 3106
Bso31I GGTCTC 2 cut(s) 1823, 1860
BsoBI CYCGRG 2 cut(s) 184, 3288
Bsp1286I GDGCHC 3 cut(s) 891, 2334, 3314
Bsp1407I TGTACA 1 cut(s) 3304
Bsp1720I GCTNAGC 1 cut(s) 66
Bsp19I CCATGG 2 cut(s) 2102, 2401
BspACI CCGC 9 cut(s) 925, 949, 1032, 1976, 2449, 2663, 3252, 3459, 3468
BspANI GGCC 9 cut(s) 178, 506, 712, 741, 1752, 2199, 2268, 2400, 3106
BspFNI CGCG 1 cut(s) 3252
BspHI TCATGA 2 cut(s) 2212, 2914
BspLI GGNNCC 8 cut(s) 58, 497, 1433, 1929, 3148, 3416, 3472, 3532
BspMAI CTGCAG 3 cut(s) 808, 1240, 1252
BspMI ACCTGC 1 cut(s) 2971
BspQI GCTCTTC 2 cut(s) 153, 2512
BspT107I GGYRCC 2 cut(s) 56, 3470
BspTNI GGTCTC 2 cut(s) 1823, 1860
BsrDI GCAATG 2 cut(s) 958, 2015
BsrGI TGTACA 1 cut(s) 3304
BsrI ACTGG 3 cut(s) 210, 606, 992
BssECI CCNNGG 9 cut(s) 1435, 1541, 1931, 2102, 2323, 2401, 2500, 3287, 3474
BssNI GRCGYC 1 cut(s) 57
BssSI CACGAG 1 cut(s) 1992
BssT1I CCWWGG 3 cut(s) 2102, 2323, 2401
Bst2BI CACGAG 1 cut(s) 1992
Bst2UI CCWGG 5 cut(s) 462, 1437, 1543, 1628, 3475
Bst4CI ACNGT 8 cut(s) 572, 1474, 1870, 2698, 2779, 2852, 2872, 3185
BstACI GRCGYC 1 cut(s) 57
BstAPI GCANNNNNTGC 2 cut(s) 1997, 3094
BstAUI TGTACA 1 cut(s) 3304
BstC8I GCNNGC 4 cut(s) 1746, 1750, 1816, 2197
BstDSI CCRYGG 2 cut(s) 2102, 2401
BstFNI CGCG 1 cut(s) 3252
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 5 cut(s) 59, 1744, 1905, 3252, 3436
BstMAI GTCTC 6 cut(s) 961, 1823, 1860, 2366, 2704, 2989
BstMCI CGRYCG 1 cut(s) 3463
BstMWI GCNNNNNNNGC 9 cut(s) 274, 332, 668, 1082, 1997, 2205, 3094, 3103, 3465
BstNI CCWGG 5 cut(s) 462, 1437, 1543, 1628, 3475
BstNSI RCATGY 3 cut(s) 1888, 2078, 2351
BstSCI CCNGG 6 cut(s) 460, 627, 1435, 1541, 1626, 3473
BstSFI CTRYAG 4 cut(s) 804, 1224, 1236, 1248
BstUI CGCG 1 cut(s) 3252
BstV2I GAAGAC 4 cut(s) 573, 689, 2739, 2838
BstX2I RGATCY 8 cut(s) 1132, 1927, 1985, 2050, 2169, 2428, 3016, 3046
BstXI CCANNNNNNTGG 1 cut(s) 1627
BstYI RGATCY 8 cut(s) 1132, 1927, 1985, 2050, 2169, 2428, 3016, 3046
Bsu36I CCTNAGG 2 cut(s) 500, 3021
BsuRI GGCC 9 cut(s) 178, 506, 712, 741, 1752, 2199, 2268, 2400, 3106
BtgI CCRYGG 2 cut(s) 2102, 2401
BtrI CACGTC 2 cut(s) 3170, 3506
BtsI GCAGTG 2 cut(s) 1533, 2190
BveI ACCTGC 1 cut(s) 2971
Cac8I GCNNGC 4 cut(s) 1746, 1750, 1816, 2197
CaiI CAGNNNCTG 1 cut(s) 1058
CciI TCATGA 2 cut(s) 2212, 2914
CfoI GCGC 5 cut(s) 59, 1744, 1905, 3252, 3436
Cfr13I GGNCC 9 cut(s) 504, 625, 710, 739, 2824, 2960, 3147, 3226, 3500
CseI GACGC 3 cut(s) 1424, 2429, 2609
Csp6I GTAC 5 cut(s) 946, 2570, 2694, 2805, 3305
CviQI GTAC 5 cut(s) 946, 2570, 2694, 2805, 3305
DinI GGCGCC 1 cut(s) 58
DrdI GACNNNNNNGTC 1 cut(s) 2984
DseDI GACNNNNNNGTC 1 cut(s) 2984
EaeI YGGCCR 1 cut(s) 2398
Ecl136II GAGCTC 2 cut(s) 889, 2332
Eco130I CCWWGG 3 cut(s) 2102, 2323, 2401
Eco147I AGGCCT 1 cut(s) 178
Eco24I GRGCYC 2 cut(s) 891, 2334
Eco31I GGTCTC 2 cut(s) 1823, 1860
Eco32I GATATC 1 cut(s) 3512
Eco47I GGWCC 6 cut(s) 625, 2824, 2960, 3147, 3226, 3500
Eco53kI GAGCTC 2 cut(s) 889, 2332
Eco81I CCTNAGG 2 cut(s) 500, 3021
Eco88I CYCGRG 2 cut(s) 184, 3288
EcoICRI GAGCTC 2 cut(s) 889, 2332
EcoO109I RGGNCCY 1 cut(s) 739
EcoRII CCWGG 5 cut(s) 460, 1435, 1541, 1626, 3473
EcoRV GATATC 1 cut(s) 3512
EcoT14I CCWWGG 3 cut(s) 2102, 2323, 2401
EcoT38I GRGCYC 2 cut(s) 891, 2334
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
ErhI CCWWGG 3 cut(s) 2102, 2323, 2401
FalI AAGNNNNNCTT 4 cut(s) 440, 472, 755, 787
FaqI GGGAC 4 cut(s) 1106, 1523, 2607, 3194
FauNDI CATATG 3 cut(s) 913, 1088, 2313
FriOI GRGCYC 2 cut(s) 891, 2334
FspI TGCGCA 1 cut(s) 3435
GlaI GCGC 5 cut(s) 58, 1743, 1904, 3251, 3435
GsuI CTGGAG 4 cut(s) 975, 1035, 3231, 3549
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 9 cut(s) 178, 506, 712, 741, 1752, 2199, 2268, 2400, 3106
HapII CCGG 5 cut(s) 48, 60, 628, 755, 3534
HgaI GACGC 3 cut(s) 1424, 2429, 2609
HhaI GCGC 5 cut(s) 59, 1744, 1905, 3252, 3436
Hin1I GRCGYC 1 cut(s) 57
Hin6I GCGC 5 cut(s) 57, 1742, 1903, 3250, 3434
HinP1I GCGC 5 cut(s) 57, 1742, 1903, 3250, 3434
HincII GTYRAC 2 cut(s) 1596, 2239
HindII GTYRAC 2 cut(s) 1596, 2239
HindIII AAGCTT 1 cut(s) 1514
HpaII CCGG 5 cut(s) 48, 60, 628, 755, 3534
HphI GGTGA 3 cut(s) 1367, 2120, 3200
Hpy166II GTNNAC 8 cut(s) 1596, 2096, 2149, 2239, 2486, 2570, 2960, 3500
Hpy8I GTNNAC 8 cut(s) 1596, 2096, 2149, 2239, 2486, 2570, 2960, 3500
Hpy99I CGWCG 2 cut(s) 2945, 3510
HpyCH4III ACNGT 8 cut(s) 572, 1474, 1870, 2698, 2779, 2852, 2872, 3185
HpyCH4IV ACGT 2 cut(s) 3169, 3505
HpyF10VI GCNNNNNNNGC 9 cut(s) 274, 332, 668, 1082, 1997, 2205, 3094, 3103, 3465
HpySE526I ACGT 2 cut(s) 3169, 3505
Hsp92I GRCGYC 1 cut(s) 57
HspAI GCGC 5 cut(s) 57, 1742, 1903, 3250, 3434
KasI GGCGCC 1 cut(s) 56
LguI GCTCTTC 2 cut(s) 153, 2512
LmnI GCTCC 8 cut(s) 62, 262, 495, 850, 1437, 1555, 1629, 3530
LweI GCATC 5 cut(s) 334, 475, 1030, 1327, 2029
MaeII ACGT 2 cut(s) 3169, 3505
MaeIII GTNAC 7 cut(s) 328, 1010, 1024, 1158, 1355, 2846, 3254
MflI RGATCY 8 cut(s) 1132, 1927, 1985, 2050, 2169, 2428, 3016, 3046
MhlI GDGCHC 3 cut(s) 891, 2334, 3314
MlsI TGGCCA 1 cut(s) 2400
MluNI TGGCCA 1 cut(s) 2400
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 8 cut(s) 143, 176, 646, 1184, 2212, 2368, 2626, 3215
MmeI TCCRAC 5 cut(s) 387, 535, 1617, 1869, 3154
Mox20I TGGCCA 1 cut(s) 2400
MroXI GAANNNNTTC 3 cut(s) 452, 816, 1066
MscI TGGCCA 1 cut(s) 2400
MslI CAYNNNNRTG 4 cut(s) 858, 2456, 2919, 3168
Msp20I TGGCCA 1 cut(s) 2400
MspA1I CMGCKG 3 cut(s) 1055, 1154, 1748
MspI CCGG 5 cut(s) 48, 60, 628, 755, 3534
MspR9I CCNGG 6 cut(s) 462, 629, 1437, 1543, 1628, 3475
Mva1269I GAATGC 1 cut(s) 2295
MvaI CCWGG 5 cut(s) 462, 1437, 1543, 1628, 3475
MvnI CGCG 1 cut(s) 3252
MwoI GCNNNNNNNGC 9 cut(s) 274, 332, 668, 1082, 1997, 2205, 3094, 3103, 3465
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 1 cut(s) 629
NcoI CCATGG 2 cut(s) 2102, 2401
NdeI CATATG 3 cut(s) 913, 1088, 2313
NlaIV GGNNCC 8 cut(s) 58, 497, 1433, 1929, 3148, 3416, 3472, 3532
NmeAIII GCCGAG 3 cut(s) 85, 652, 1912
NmuCI GTSAC 3 cut(s) 1010, 1355, 2846
NsbI TGCGCA 1 cut(s) 3435
NspI RCATGY 3 cut(s) 1888, 2078, 2351
PacI TTAATTAA 1 cut(s) 1662
PagI TCATGA 2 cut(s) 2212, 2914
PceI AGGCCT 1 cut(s) 178
PciI ACATGT 2 cut(s) 1884, 2347
PciSI GCTCTTC 2 cut(s) 153, 2512
PctI GAATGC 1 cut(s) 2295
PdmI GAANNNNTTC 3 cut(s) 452, 816, 1066
PflFI GACNNNGTC 1 cut(s) 3504
PflMI CCANNNNNTGG 3 cut(s) 707, 1007, 1058
PfoI TCCNGGA 1 cut(s) 460
PleI GAGTC 8 cut(s) 142, 176, 646, 1183, 2211, 2368, 2626, 3214
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 8 cut(s) 142, 176, 646, 1183, 2211, 2368, 2626, 3214
PscI ACATGT 2 cut(s) 1884, 2347
PshBI ATTAAT 2 cut(s) 1662, 1898
Psp124BI GAGCTC 2 cut(s) 891, 2334
Psp6I CCWGG 5 cut(s) 460, 1435, 1541, 1626, 3473
PspGI CCWGG 5 cut(s) 460, 1435, 1541, 1626, 3473
PspN4I GGNNCC 8 cut(s) 58, 497, 1433, 1929, 3148, 3416, 3472, 3532
PspPI GGNCC 9 cut(s) 504, 625, 710, 739, 2824, 2960, 3147, 3226, 3500
PstI CTGCAG 3 cut(s) 808, 1240, 1252
PstNI CAGNNNCTG 1 cut(s) 1058
PsuI RGATCY 8 cut(s) 1132, 1927, 1985, 2050, 2169, 2428, 3016, 3046
PsyI GACNNNGTC 1 cut(s) 3504
PvuII CAGCTG 3 cut(s) 1055, 1154, 1748
RsaI GTAC 5 cut(s) 947, 2571, 2695, 2806, 3306
RsaNI GTAC 5 cut(s) 946, 2570, 2694, 2805, 3305
RseI CAYNNNNRTG 4 cut(s) 858, 2456, 2919, 3168
SacI GAGCTC 2 cut(s) 891, 2334
SapI GCTCTTC 2 cut(s) 153, 2512
Sau96I GGNCC 9 cut(s) 504, 625, 710, 739, 2824, 2960, 3147, 3226, 3500
SchI GAGTC 8 cut(s) 143, 176, 646, 1184, 2212, 2368, 2626, 3215
ScrFI CCNGG 6 cut(s) 462, 629, 1437, 1543, 1628, 3475
SduI GDGCHC 3 cut(s) 891, 2334, 3314
SfaNI GCATC 5 cut(s) 334, 475, 1030, 1327, 2029
SfcI CTRYAG 4 cut(s) 804, 1224, 1236, 1248
SfoI GGCGCC 1 cut(s) 58
SinI GGWCC 6 cut(s) 625, 2824, 2960, 3147, 3226, 3500
SmiMI CAYNNNNRTG 4 cut(s) 858, 2456, 2919, 3168
SmlI CTYRAG 6 cut(s) 110, 289, 1826, 2069, 2678, 3050
SmoI CTYRAG 6 cut(s) 110, 289, 1826, 2069, 2678, 3050
SpeI ACTAGT 1 cut(s) 3485
SseBI AGGCCT 1 cut(s) 178
SsiI CCGC 9 cut(s) 925, 949, 1032, 1976, 2449, 2663, 3252, 3459, 3468
SspDI GGCGCC 1 cut(s) 56
SspI AATATT 4 cut(s) 1684, 1705, 1804, 1896
SstI GAGCTC 2 cut(s) 891, 2334
StuI AGGCCT 1 cut(s) 178
StyD4I CCNGG 6 cut(s) 460, 627, 1435, 1541, 1626, 3473
StyI CCWWGG 3 cut(s) 2102, 2323, 2401
TaaI ACNGT 8 cut(s) 572, 1474, 1870, 2698, 2779, 2852, 2872, 3185
TaiI ACGT 2 cut(s) 3172, 3508
TaqI TCGA 5 cut(s) 132, 137, 1461, 2635, 3508
TaqII GACCGA 2 cut(s) 1842, 2514
TatI WGTACW 2 cut(s) 2569, 3304
TauI GCSGC 2 cut(s) 2666, 3471
TseFI GTSAC 3 cut(s) 1010, 1355, 2846
Tsp45I GTSAC 3 cut(s) 1010, 1355, 2846
TspGWI ACGGA 1 cut(s) 1997
Tth111I GACNNNGTC 1 cut(s) 3504
Van91I CCANNNNNTGG 3 cut(s) 707, 1007, 1058
VpaK11BI GGWCC 6 cut(s) 625, 2824, 2960, 3147, 3226, 3500
VspI ATTAAT 2 cut(s) 1662, 1898
XapI RAATTY 6 cut(s) 311, 646, 1408, 1492, 1921, 2219
XceI RCATGY 3 cut(s) 1888, 2078, 2351
XcmI CCANNNNNNNNNTGG 1 cut(s) 1549
XmaJI CCTAGG 1 cut(s) 2323
XmnI GAANNNNTTC 3 cut(s) 452, 816, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.