pycom07g17420

Regulator of nonsense transcripts

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007975
Physical Location & Seq
Reverse (-)
19979567 .. 19988970
9404 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g17420.5

Sequence Viewer

Length: 3447 bp
ATGGCTCTTCGTCAAAGCAATCTGAACCCCGAGCGGCCTGACACAGGATTCCTTAGGACACTGGATTCTAGCATTAAGCGCAACACAGCGGTTATTAAAAAACTGAAGCAGATAAACGAAGAGCAGCGGGAAGGGTTGATGGATGACTTGAGAGTTGTCAACCTGAGCAAATTTGTCAGTGAAGCTGTTACTGCTATTTGTGATGCCAAGCTTAGATCGTCAGACATACAAGCAGCAGTCCAGATCTGCTCTCTGCTCCATCAAAGGTACAAAGACTTCTCCCCAACTCTGCTTCAAGGTCTTTTGAAAGTCTTTTTTCCTGGGAAGTCTGGGGATGACTTAGAAGCAGACAAGAACTTGAGGGCCATGAAGAAGCGAAGCACTTTGAAACTCCTGTTGGAACTTTTCTTTGTTGGAGTTATAGAAGATGGTGGTATTTTCGTGAATATCATTAAGGATCTTGCCAGTGGAGAACACTTAAGGGATCGGGATACTACTCAGACAAATTTGACTCTTCTTGCTAGTTTTGCTCGTCAGGGAAGAATCTTTCTTGGCCTTCCACTCGCTGGGCCAGAACTTCATGAAGAGTTTTTTAAGGGCCTTAATATTACGACAGATCAAAAGAAATTCTTCAGGAAGGCATTTCAAACATATTCTGCTGCTGCTGCCGAACTGCTGCAATCCGAGCATACTTCACTTCGCCAAATGGAGCATGAAAATGCTAAAATCCTTAATGCTAAAGGAGAGCTCAGTGATGAAAGTGTCTCATCATACGAGAAGCTGCGGAAATCTTATGAACATTTGTACCGCAATGTCTCATCTTTAGCAGAAGCACTTGATATGCAGCCCCCAGTAATGCCAGAGGATGGTCACACAACAAGGGTTACTAGTGGGGAGGATGCTTCATCTCCTGTTGCTGGAAAGGATTCTTCGGCACTCGAAGCCATATGGGATGATGAAGATACAAGGGCTTTCTATGAATGCTTACCAGATCTTAGAGCATTTGTTCCGGCAGTATTATTGGGAGAAGCAGAGTCTAAAAGCATTGATCAATCTGCAAAGACCCAGGAACAACCAACTGAACCCGCACTTGAATCAGATCAAACTCAACAAATTACAGAAGATACTGGAGAGGCTTCTACAGATGTTGGTGCTTTACAAGAGGCCAAAAGCACAGAGAAAGGAAAGGACAAGGAAGAAAAAGATAAAGACAAGATCAAAGATCCAGACAAAGAGAAAGTTGACAGAAAAGGAGAGAATGACAAAGAGAAGCTCAAAAGTATTGAAGGGACAAATTTGGATGCTTTACTGCAGAGGCTTCCTGGTTGCGTGAGCCGTGATCTTATTGATCAGTTGACTGTGGAGTTTTGTTATTTAAATTCAAAAGCAAATCGGAAAAAGCTTGTGAGGGCATTATTTAATGTCCCTAGAACATCATTGGAGCTACTAGCTTACTACTCGCGTTTGGTTGCCACACTCTCGACTTGTATGAAGGACGTCTCATCCATGCTCCTAGCGATGTTGGAAGAAGAGTTCAACTTCTTAATTAATAAAAAGGACCAAATGAACATTGAAACGAAGATTAGGAACATTAGATTTATTGGAGAACTCTGCAAGTTTAAGATTGCACCAGCTGGTCTTGTCTTTAGTTGTTTGAAGGCTTGTTTGGATGATTTCAGTCATCATAACATTGATGTTGCTTGCAATCTTCTTGAGACTTGTGGGCGTTTTCTGTATCGGTCTCCTGAAACTACTGTGCGGATGGCTAACATGTTGGAAATATTAATGCGATTGAAGAACGTAAAAAATTTAGACCCTCGGCACAGCACCCTTGTTGAAAATGCATACTACCTTTGCAAACCACCTGAGAGATCTGCACGGGTCACTAAAGTCCGTCCTCCATTGCATCAGTATATAAGAAAGTTGCTATTCTCAGATCTTGATAAGTCTACCATAGAGCATGTGCTCAGGCAACTGCGTAAATTACCATGGGGTGAATGTGAGCCGTATCTTTTAAAATGCTTTCTGAAGGTTCACAAAGGGAAGTATGGTCAGATCCATTTGATTGCTTCTCTCACCGCTGGTTTGAGCCGCTATCATGATGAATTTGCTGTATCTGTTGTTGATGAGGTTCTAGAGGAGATACGGCTTGGCCTAGAATTAAATGAGTATGGGATGCAGCAAAGACGGATTGCCCATATGCGATTTTTAGGAGAGCTATACAACTATGAGCATGTAGATTCTTCTGTTATTTTTGAAACTCTGTACTTGGTTCTTGCCTTTGGCCATGGCACACCAGAGCAAGATGTACTCGATCCACCAGAAGATTGTTTCCGCATGAGGATGGTTATTACTCTTCTTGAAACCTGCGGGCACTACTTTGACCGAGGCTCTTCCAAGAGGAAACTTGATCGATTCTTGATGCACTTTCAGAGATATATACTAAGTAAAGGTGTACTACCGCTTGATGTGGAATTTGACTTACAGGACTTATTTGCTGATCTACGCCCCAACATGGCTCGGTACTCTTCCATTGAAGAAGTTAATGCAGCTCTTTTAGAACTTGAGGAGCGTGATCGTACTGTCTCCATTGAGAAGCACTCTGACACTGAAAAGCCTTCAAGGAGGAATACCTCCGATGAAATGTCAGTGAATGGAAAGAGTATTGTAAATGGAACCGGGGAAAATGGTGTAGTTCATGGAAACCTTGGAGATAGTGACAGTGATTCGGGAAGTGGCACCATTGATCGAGATAGACATAATGAAGAAGGATTGGGTGAAGAGAATCATGATGGTGGATCTGACAGTGATGAGGATGATGATGATGGTGGTGGACCGGCATCCGATGAGGACGATGAAGTCCATGTCAGACAGAGGGTTGCTGAGGTCGACCCTCAAGAAGCAGAAAAGTTTGAGCTGGATCTCAAGGCTGTTATGCAGGAAAGCATGGAGCAACGTAGGCTAGAGCTGAGAGGTCGACCAACATTAAACATGATGATACCGATGAATGTGTTTGAGGGGTCCACCAAGGACCATCATGGAAGAGGGTTTGGAGGGGAAAGTGGAGATGAGGCATTGGATGAAGAGTCTGGAGGAAGCAAGGAGGTCCAGGTGAAAGTTCTTGTCAAGCGTGGTAACAAGCAACAAACAAAGCAGATGTACATCCCTCGGGATTGCTCACTTATCCAGAGTACGAAACAGAAAGAAGCAGAAGAGCTTGAAGAGAAACAAGACATCAAGAGGTTGGTTTTGGAATACAATGACAGAGAAGAGGAGGAGCTTAATGGATTGGGGAACCAGACTCTGAACTATATGCAGAGTGGCGGTAATAGACTTGCCGGCCGTGGCAGCACTTGGGAAGGAGCTAGCGGTAGAGGTGGTGGAGCACGTCATAGATATCACAGTAGTTATTCCGGTGGGGGAGCTTTCTACAGCAGGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0031503 GO:0031974 GO:0031981 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034655 GO:0035145 GO:0035690 GO:0035770 GO:0036464 GO:0042162 GO:0042221 GO:0042493 GO:0042742 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046677 GO:0046700 GO:0046907 GO:0048519 GO:0048571 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071426 GO:0071427 GO:0071446 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
MIF4G Upf2

Protein Analysis

1149

Amino Acids

129.63

Weight (kDa)

5.44

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2858
AatII GACGTC 1 cut(s) 1500
Acc36I ACCTGC 1 cut(s) 2375
AccB1I GGYRCC 1 cut(s) 2738
AccB7I CCANNNNNTGG 2 cut(s) 566, 866
AccBSI CCGCTC 1 cut(s) 34
AccI GTMKAC 3 cut(s) 1949, 2889, 2977
AccII CGCG 1 cut(s) 1462
AclWI GGATC 7 cut(s) 465, 492, 1217, 2050, 2309, 2806, 2928
AcoI YGGCCR 2 cut(s) 2284, 3340
AcsI RAATTY 8 cut(s) 170, 505, 626, 1294, 1378, 1807, 2105, 2474
AcuI CTGAAG 3 cut(s) 125, 616, 2048
AcyI GRCGYC 1 cut(s) 1497
AfaI GTAC 9 cut(s) 269, 806, 2267, 2310, 2457, 2525, 2581, 3161, 3193
AfiI CCNNNNNNNGG 5 cut(s) 44, 566, 866, 917, 2515
AflII CTTAAG 1 cut(s) 478
AflIII ACRYGT 1 cut(s) 1770
AhlI ACTAGT 1 cut(s) 887
AjiI CACGTC 1 cut(s) 3389
AjnI CCWGG 4 cut(s) 319, 1065, 1321, 3108
AjuI GAANNNNNNNTTGG 4 cut(s) 380, 412, 1649, 1681
AloI GAACNNNNNNTCC 2 cut(s) 1517, 1549
Alw21I GWGCWC 3 cut(s) 750, 1968, 3388
Alw26I GTCTC 6 cut(s) 769, 820, 1504, 1709, 1746, 2590
AlwI GGATC 7 cut(s) 465, 492, 1217, 2050, 2309, 2806, 2928
AlwNI CAGNNNCTG 1 cut(s) 3304
Ama87I CYCGRG 2 cut(s) 29, 3168
AoxI GGCC 9 cut(s) 35, 363, 553, 569, 598, 1164, 2152, 2284, 3340
ApoI RAATTY 8 cut(s) 170, 505, 626, 1294, 1378, 1807, 2105, 2474
AseI ATTAAT 2 cut(s) 1548, 1784
Asp700I GAANNNNTTC 2 cut(s) 629, 925
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 8 cut(s) 363, 569, 598, 1558, 2834, 3021, 3031, 3106
AsuC2I CCSGG 1 cut(s) 2680
AsuHPI GGTGA 4 cut(s) 2006, 2068, 2789, 3124
AsuNHI GCTAGC 1 cut(s) 3365
AvaI CYCGRG 2 cut(s) 29, 3168
AvaII GGWCC 5 cut(s) 1558, 2834, 3021, 3031, 3106
AxyI CCTNAGG 1 cut(s) 53
BaeGI GKGCMC 1 cut(s) 2376
BaeI ACNNNNGTAYC 2 cut(s) 788, 821
BalI TGGCCA 1 cut(s) 2286
BanI GGYRCC 1 cut(s) 2738
BanII GRGCYC 1 cut(s) 750
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
Bbv12I GWGCWC 3 cut(s) 750, 1968, 3388
BbvCI CCTCAGC 1 cut(s) 2883
BccI CCATC 9 cut(s) 133, 267, 422, 860, 1756, 2338, 2786, 2819, 3042
BceAI ACGGC 4 cut(s) 1320, 1990, 2162, 3327
BciT130I CCWGG 4 cut(s) 321, 1067, 1323, 3110
BciVI GTATCC 1 cut(s) 484
BclI TGATCA 2 cut(s) 1048, 1348
BcnI CCSGG 1 cut(s) 2680
BcoDI GTCTC 6 cut(s) 769, 820, 1504, 1709, 1746, 2590
BcuI ACTAGT 1 cut(s) 887
BfmI CTRYAG 3 cut(s) 1140, 1310, 3430
BfrI CTTAAG 1 cut(s) 478
BfuAI ACCTGC 1 cut(s) 2375
BfuI GTATCC 1 cut(s) 484
BglII AGATCT 4 cut(s) 243, 991, 1871, 1936
Bme1390I CCNGG 5 cut(s) 321, 1067, 1323, 2680, 3110
Bme18I GGWCC 5 cut(s) 1558, 2834, 3021, 3031, 3106
BmeT110I CYCGRG 2 cut(s) 29, 3168
BmgBI CACGTC 1 cut(s) 3389
BmgT120I GGNCC 8 cut(s) 363, 569, 598, 1558, 2834, 3021, 3031, 3106
BmiI GGNNCC 4 cut(s) 2677, 2740, 3022, 3296
BmrFI CCNGG 5 cut(s) 321, 1067, 1323, 2680, 3110
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 7 cut(s) 193, 889, 1291, 1915, 2166, 2412, 2849
BmtI GCTAGC 1 cut(s) 3369
BmuI ACTGGG 1 cut(s) 845
BplI GAGNNNNNCTC 2 cut(s) 2585, 2617
BpmI CTGGAG 2 cut(s) 1149, 3111
Bpu10I CCTNAGC 3 cut(s) 164, 1967, 2883
BpuEI CTTGAG 6 cut(s) 169, 379, 1733, 2585, 2880, 2909
BpuMI CCSGG 1 cut(s) 2680
Bsa29I ATCGAT 1 cut(s) 2413
BsaBI GATNNNNATC 1 cut(s) 3161
BsaHI GRCGYC 1 cut(s) 1497
BsaI GGTCTC 1 cut(s) 1746
BsaWI WCCGGW 1 cut(s) 3413
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 566, 866, 917, 2515
Bse118I RCCGGY 2 cut(s) 2836, 3338
Bse1I ACTGG 4 cut(s) 66, 465, 851, 1132
Bse21I CCTNAGG 1 cut(s) 53
Bse3DI GCAATG 2 cut(s) 817, 1901
Bse8I GATNNNNATC 1 cut(s) 3161
BseBI CCWGG 4 cut(s) 321, 1067, 1323, 3110
BseCI ATCGAT 1 cut(s) 2413
BseJI GATNNNNATC 1 cut(s) 3161
BseLI CCNNNNNNNGG 5 cut(s) 44, 566, 866, 917, 2515
BseMI GCAATG 2 cut(s) 817, 1901
BseMII CTCAG 8 cut(s) 155, 512, 763, 1857, 1947, 1981, 2874, 2960
BseNI ACTGG 4 cut(s) 66, 465, 851, 1132
BseRI GAGGAG 4 cut(s) 2153, 2582, 3287, 3290
BseSI GKGCMC 1 cut(s) 2376
BseX3I CGGCCG 1 cut(s) 3340
BseYI CCCAGC 1 cut(s) 566
BsgI GTGCAG 1 cut(s) 1860
Bsh1236I CGCG 1 cut(s) 1462
Bsh1285I CGRYCG 1 cut(s) 3343
BshFI GGCC 9 cut(s) 37, 365, 555, 571, 600, 1166, 2154, 2286, 3342
BshNI GGYRCC 1 cut(s) 2738
BshVI ATCGAT 1 cut(s) 2413
BsiEI CGRYCG 1 cut(s) 3343
BsiHKAI GWGCWC 3 cut(s) 750, 1968, 3388
BsiHKCI CYCGRG 2 cut(s) 29, 3168
BsiSI CCGG 5 cut(s) 1010, 2679, 2837, 3339, 3414
BslFI GGGAC 2 cut(s) 1303, 1409
BslI CCNNNNNNNGG 5 cut(s) 44, 566, 866, 917, 2515
BsmAI GTCTC 6 cut(s) 769, 820, 1504, 1709, 1746, 2590
BsmBI CGTCTC 1 cut(s) 1504
BsmFI GGGAC 2 cut(s) 1303, 1409
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 9 cut(s) 37, 365, 555, 571, 600, 1166, 2154, 2286, 3342
Bso31I GGTCTC 1 cut(s) 1746
BsoBI CYCGRG 2 cut(s) 29, 3168
Bsp1286I GDGCHC 4 cut(s) 750, 1968, 2376, 3388
Bsp1407I TGTACA 1 cut(s) 3159
Bsp19I CCATGG 2 cut(s) 1988, 2287
BspANI GGCC 9 cut(s) 37, 365, 555, 571, 600, 1166, 2154, 2286, 3342
BspCNI CTCAG 8 cut(s) 156, 511, 762, 1858, 1946, 1980, 2875, 2961
BspDI ATCGAT 1 cut(s) 2413
BspFNI CGCG 1 cut(s) 1462
BspHI TCATGA 3 cut(s) 580, 2098, 2788
BspLI GGNNCC 4 cut(s) 2677, 2740, 3022, 3296
BspMAI CTGCAG 1 cut(s) 1314
BspMI ACCTGC 1 cut(s) 2375
BspOI GCTAGC 1 cut(s) 3369
BspPI GGATC 7 cut(s) 465, 492, 1217, 2050, 2309, 2806, 2928
BspQI GCTCTTC 4 cut(s) 12, 114, 2398, 3207
BspT107I GGYRCC 1 cut(s) 2738
BspTI CTTAAG 1 cut(s) 478
BspTNI GGTCTC 1 cut(s) 1746
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 2 cut(s) 817, 1901
BsrFI RCCGGY 2 cut(s) 2836, 3338
BsrGI TGTACA 1 cut(s) 3159
BsrI ACTGG 4 cut(s) 66, 465, 851, 1132
BssAI RCCGGY 2 cut(s) 2836, 3338
BssNI GRCGYC 1 cut(s) 1497
BssT1I CCWWGG 4 cut(s) 1988, 2287, 2707, 3027
Bst2UI CCWGG 4 cut(s) 321, 1067, 1323, 3110
Bst4CI ACNGT 6 cut(s) 1360, 1756, 2584, 2723, 2807, 3404
BstACI GRCGYC 1 cut(s) 1497
BstAFI CTTAAG 1 cut(s) 478
BstAUI TGTACA 1 cut(s) 3159
BstC8I GCNNGC 4 cut(s) 1702, 2372, 3340, 3367
BstDSI CCRYGG 3 cut(s) 1988, 2287, 3343
BstENI CCTNNNNNAGG 1 cut(s) 42
BstFNI CGCG 1 cut(s) 1462
BstHHI GCGC 1 cut(s) 81
BstMAI GTCTC 6 cut(s) 769, 820, 1504, 1709, 1746, 2590
BstMCI CGRYCG 1 cut(s) 3343
BstMWI GCNNNNNNNGC 8 cut(s) 78, 191, 527, 665, 685, 941, 2959, 3348
BstNI CCWGG 4 cut(s) 321, 1067, 1323, 3110
BstNSI RCATGY 3 cut(s) 1774, 1964, 2237
BstSCI CCNGG 5 cut(s) 319, 1065, 1321, 2678, 3108
BstSFI CTRYAG 3 cut(s) 1140, 1310, 3430
BstSLI GKGCMC 1 cut(s) 2376
BstUI CGCG 1 cut(s) 1462
BstX2I RGATCY 9 cut(s) 243, 457, 991, 1222, 1871, 1936, 2055, 2798, 2920
BstYI RGATCY 9 cut(s) 243, 457, 991, 1222, 1871, 1936, 2055, 2798, 2920
BstZI CGGCCG 1 cut(s) 3340
Bsu15I ATCGAT 1 cut(s) 2413
Bsu36I CCTNAGG 1 cut(s) 53
BsuI GTATCC 1 cut(s) 484
BsuRI GGCC 9 cut(s) 37, 365, 555, 571, 600, 1166, 2154, 2286, 3342
BsuTUI ATCGAT 1 cut(s) 2413
BtgI CCRYGG 3 cut(s) 1988, 2287, 3343
BtgZI GCGATG 1 cut(s) 1532
BtrI CACGTC 1 cut(s) 3389
BtsIMutI CAGTG 8 cut(s) 59, 184, 472, 757, 2607, 2655, 2728, 2812
BveI ACCTGC 1 cut(s) 2375
Cac8I GCNNGC 4 cut(s) 1702, 2372, 3340, 3367
CaiI CAGNNNCTG 1 cut(s) 3304
CciI TCATGA 3 cut(s) 580, 2098, 2788
CfoI GCGC 1 cut(s) 81
Cfr10I RCCGGY 2 cut(s) 2836, 3338
Cfr13I GGNCC 8 cut(s) 363, 569, 598, 1558, 2834, 3021, 3031, 3106
ClaI ATCGAT 1 cut(s) 2413
Csp6I GTAC 9 cut(s) 268, 805, 2266, 2309, 2456, 2524, 2580, 3160, 3192
CviQI GTAC 9 cut(s) 268, 805, 2266, 2309, 2456, 2524, 2580, 3160, 3192
DraI TTTAAA 2 cut(s) 1377, 2016
DrdI GACNNNNNNGTC 1 cut(s) 2858
DseDI GACNNNNNNGTC 1 cut(s) 2858
EaeI YGGCCR 2 cut(s) 2284, 3340
EagI CGGCCG 1 cut(s) 3340
Ecl136II GAGCTC 1 cut(s) 748
EclXI CGGCCG 1 cut(s) 3340
Eco130I CCWWGG 4 cut(s) 1988, 2287, 2707, 3027
Eco24I GRGCYC 1 cut(s) 750
Eco31I GGTCTC 1 cut(s) 1746
Eco32I GATATC 1 cut(s) 3398
Eco47I GGWCC 5 cut(s) 1558, 2834, 3021, 3031, 3106
Eco52I CGGCCG 1 cut(s) 3340
Eco53kI GAGCTC 1 cut(s) 748
Eco57I CTGAAG 3 cut(s) 125, 616, 2048
Eco81I CCTNAGG 1 cut(s) 53
Eco88I CYCGRG 2 cut(s) 29, 3168
EcoICRI GAGCTC 1 cut(s) 748
EcoNI CCTNNNNNAGG 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 598
EcoRII CCWGG 4 cut(s) 319, 1065, 1321, 3108
EcoRV GATATC 1 cut(s) 3398
EcoT14I CCWWGG 4 cut(s) 1988, 2287, 2707, 3027
EcoT22I ATGCAT 1 cut(s) 1846
EcoT38I GRGCYC 1 cut(s) 750
ErhI CCWWGG 4 cut(s) 1988, 2287, 2707, 3027
Esp3I CGTCTC 1 cut(s) 1504
FalI AAGNNNNNCTT 2 cut(s) 614, 646
FaqI GGGAC 2 cut(s) 1303, 1409
FauI CCCGC 3 cut(s) 120, 1093, 2363
FauNDI CATATG 2 cut(s) 947, 2199
FbaI TGATCA 2 cut(s) 1048, 1348
FblI GTMKAC 3 cut(s) 1949, 2889, 2977
FriOI GRGCYC 1 cut(s) 750
GlaI GCGC 1 cut(s) 80
GsaI CCCAGC 1 cut(s) 570
GsuI CTGGAG 2 cut(s) 1149, 3111
HaeIII GGCC 9 cut(s) 37, 365, 555, 571, 600, 1166, 2154, 2286, 3342
HapII CCGG 5 cut(s) 1010, 2679, 2837, 3339, 3414
HhaI GCGC 1 cut(s) 81
Hin1I GRCGYC 1 cut(s) 1497
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HincII GTYRAC 5 cut(s) 160, 1243, 1356, 2890, 2978
HindII GTYRAC 5 cut(s) 160, 1243, 1356, 2890, 2978
HindIII AAGCTT 2 cut(s) 209, 1400
HpaII CCGG 5 cut(s) 1010, 2679, 2837, 3339, 3414
HphI GGTGA 4 cut(s) 2006, 2068, 2789, 3124
HpyCH4III ACNGT 6 cut(s) 1360, 1756, 2584, 2723, 2807, 3404
HpyCH4IV ACGT 4 cut(s) 1497, 1800, 2956, 3388
HpyF10VI GCNNNNNNNGC 8 cut(s) 78, 191, 527, 665, 685, 941, 2959, 3348
HpySE526I ACGT 4 cut(s) 1497, 1800, 2956, 3388
Hsp92I GRCGYC 1 cut(s) 1497
HspAI GCGC 1 cut(s) 79
KroI GCCGGC 1 cut(s) 3338
KroNI GCCGGC 1 cut(s) 3340
Ksp22I TGATCA 2 cut(s) 1048, 1348
LguI GCTCTTC 4 cut(s) 12, 114, 2398, 3207
LweI GCATC 7 cut(s) 193, 889, 1291, 1915, 2166, 2412, 2849
MaeII ACGT 4 cut(s) 1497, 1800, 2956, 3388
MaeIII GTNAC 6 cut(s) 187, 869, 883, 1882, 2717, 3134
MbiI CCGCTC 1 cut(s) 34
MflI RGATCY 9 cut(s) 243, 457, 991, 1222, 1871, 1936, 2055, 2798, 2920
MhlI GDGCHC 4 cut(s) 750, 1968, 2376, 3388
MlsI TGGCCA 1 cut(s) 2286
MluNI TGGCCA 1 cut(s) 2286
MlyI GAGTC 4 cut(s) 505, 1043, 3095, 3295
MmeI TCCRAC 4 cut(s) 378, 394, 1503, 1755
Mox20I TGGCCA 1 cut(s) 2286
Mph1103I ATGCAT 1 cut(s) 1846
MroNI GCCGGC 1 cut(s) 3338
MroXI GAANNNNTTC 2 cut(s) 629, 925
MscI TGGCCA 1 cut(s) 2286
MslI CAYNNNNRTG 3 cut(s) 717, 2342, 2793
Msp20I TGGCCA 1 cut(s) 2286
MspA1I CMGCKG 4 cut(s) 89, 127, 1634, 2081
MspCI CTTAAG 1 cut(s) 478
MspI CCGG 5 cut(s) 1010, 2679, 2837, 3339, 3414
MspR9I CCNGG 5 cut(s) 321, 1067, 1323, 2680, 3110
Mva1269I GAATGC 1 cut(s) 986
MvaI CCWGG 4 cut(s) 321, 1067, 1323, 3110
MvnI CGCG 1 cut(s) 1462
MwoI GCNNNNNNNGC 8 cut(s) 78, 191, 527, 665, 685, 941, 2959, 3348
NaeI GCCGGC 1 cut(s) 3340
NciI CCSGG 1 cut(s) 2680
NcoI CCATGG 2 cut(s) 1988, 2287
NdeI CATATG 2 cut(s) 947, 2199
NgoMIV GCCGGC 1 cut(s) 3338
NheI GCTAGC 1 cut(s) 3365
NlaIV GGNNCC 4 cut(s) 2677, 2740, 3022, 3296
NmeAIII GCCGAG 1 cut(s) 1798
NmuCI GTSAC 3 cut(s) 869, 1882, 2717
NsiI ATGCAT 1 cut(s) 1846
NspI RCATGY 3 cut(s) 1774, 1964, 2237
PacI TTAATTAA 1 cut(s) 1548
PagI TCATGA 3 cut(s) 580, 2098, 2788
PciI ACATGT 1 cut(s) 1770
PciSI GCTCTTC 4 cut(s) 12, 114, 2398, 3207
PctI GAATGC 1 cut(s) 986
PdiI GCCGGC 1 cut(s) 3340
PdmI GAANNNNTTC 2 cut(s) 629, 925
PfeI GAWTC 9 cut(s) 48, 65, 543, 926, 1094, 2240, 2415, 2726, 2785
PflMI CCANNNNNTGG 2 cut(s) 566, 866
PleI GAGTC 4 cut(s) 505, 1042, 3094, 3295
PpsI GAGTC 4 cut(s) 505, 1042, 3094, 3295
PscI ACATGT 1 cut(s) 1770
PshBI ATTAAT 2 cut(s) 1548, 1784
Psp124BI GAGCTC 1 cut(s) 750
Psp6I CCWGG 4 cut(s) 319, 1065, 1321, 3108
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 4 cut(s) 319, 1065, 1321, 3108
PspN4I GGNNCC 4 cut(s) 2677, 2740, 3022, 3296
PspPI GGNCC 8 cut(s) 363, 569, 598, 1558, 2834, 3021, 3031, 3106
PstI CTGCAG 1 cut(s) 1314
PstNI CAGNNNCTG 1 cut(s) 3304
PsuI RGATCY 9 cut(s) 243, 457, 991, 1222, 1871, 1936, 2055, 2798, 2920
PvuII CAGCTG 1 cut(s) 1634
RsaI GTAC 9 cut(s) 269, 806, 2267, 2310, 2457, 2525, 2581, 3161, 3193
RsaNI GTAC 9 cut(s) 268, 805, 2266, 2309, 2456, 2524, 2580, 3160, 3192
RseI CAYNNNNRTG 3 cut(s) 717, 2342, 2793
SacI GAGCTC 1 cut(s) 750
SalI GTCGAC 2 cut(s) 2888, 2976
SapI GCTCTTC 4 cut(s) 12, 114, 2398, 3207
Sau96I GGNCC 8 cut(s) 363, 569, 598, 1558, 2834, 3021, 3031, 3106
SchI GAGTC 4 cut(s) 505, 1043, 3095, 3295
ScrFI CCNGG 5 cut(s) 321, 1067, 1323, 2680, 3110
SduI GDGCHC 4 cut(s) 750, 1968, 2376, 3388
SfaNI GCATC 7 cut(s) 193, 889, 1291, 1915, 2166, 2412, 2849
SfcI CTRYAG 3 cut(s) 1140, 1310, 3430
SinI GGWCC 5 cut(s) 1558, 2834, 3021, 3031, 3106
SmiI ATTTAAAT 1 cut(s) 1377
SmiMI CAYNNNNRTG 3 cut(s) 717, 2342, 2793
SmlI CTYRAG 7 cut(s) 148, 358, 478, 1712, 2564, 2895, 2924
SmoI CTYRAG 7 cut(s) 148, 358, 478, 1712, 2564, 2895, 2924
SpeI ACTAGT 1 cut(s) 887
SspI AATATT 2 cut(s) 607, 1782
SstI GAGCTC 1 cut(s) 750
StyD4I CCNGG 5 cut(s) 319, 1065, 1321, 2678, 3108
StyI CCWWGG 4 cut(s) 1988, 2287, 2707, 3027
SwaI ATTTAAAT 1 cut(s) 1377
TaaI ACNGT 6 cut(s) 1360, 1756, 2584, 2723, 2807, 3404
TaiI ACGT 4 cut(s) 1500, 1803, 2959, 3391
TaqI TCGA 7 cut(s) 939, 1481, 2313, 2413, 2749, 2889, 2977
TaqII GACCGA 2 cut(s) 1728, 2400
TatI WGTACW 4 cut(s) 2265, 2308, 2455, 3159
TauI GCSGC 2 cut(s) 37, 2094
TfiI GAWTC 9 cut(s) 48, 65, 543, 926, 1094, 2240, 2415, 2726, 2785
TscAI CASTG 8 cut(s) 66, 184, 472, 757, 2614, 2655, 2728, 2812
TseFI GTSAC 3 cut(s) 869, 1882, 2717
Tsp45I GTSAC 3 cut(s) 869, 1882, 2717
TspGWI ACGGA 2 cut(s) 1883, 2203
TspRI CASTG 8 cut(s) 66, 184, 472, 757, 2614, 2655, 2728, 2812
Van91I CCANNNNNTGG 2 cut(s) 566, 866
Vha464I CTTAAG 1 cut(s) 478
VpaK11BI GGWCC 5 cut(s) 1558, 2834, 3021, 3031, 3106
VspI ATTAAT 2 cut(s) 1548, 1784
XagI CCTNNNNNAGG 1 cut(s) 42
XapI RAATTY 8 cut(s) 170, 505, 626, 1294, 1378, 1807, 2105, 2474
XbaI TCTAGA 1 cut(s) 2134
XceI RCATGY 3 cut(s) 1774, 1964, 2237
XmiI GTMKAC 3 cut(s) 1949, 2889, 2977
XmnI GAANNNNTTC 2 cut(s) 629, 925
ZraI GACGTC 1 cut(s) 1498
Zsp2I ATGCAT 1 cut(s) 1846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.