MD07G1181100.v1.1

Regulator of nonsense transcripts

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007975
Physical Location & Seq
Reverse (-)
26100511 .. 26117139
16629 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1181100.v1.1.491

Sequence Viewer

Length: 3558 bp
ATGGATCACCATGAGGAGGAATGCCGTGCTGGCAATGAACCTCATGGGAAGCAAGATGATGAGGAAGCTGCTGCTCGGCTTGAGGAAATCAGGAAGTCTATTGAGGGAAAGATGGCTCTTCGTCAAAGCAATCTGAACCCCGAGCGGCCTGACACAGGATTCCTTAGGACACTGGATTCTAGCATTAAGCGCAACACAGCGGTTATTAAAAAACTGAAGCAGATAAATGAAGAGCAGCGGGAAGGGTTGATGGATGACTTGAGAGTTGTCAACCTGAGCAAATTTGTCAGTGAAGCTGTTACTGCTATTTGTGATGCCAAGCTTAGATCGTCAGACATACAAGCAGCAGTCCAGATCTGCTCTCTGCTCCATCAAAGGTACAAAGACTTCTCCCCAACTCTGCTTCAAGGTCTTTTGAAAGTCTTCTTTCCAGGGAAGTCTGGGGATGACTTAGAAGCAGACAAGAACCTGAGGGCCATGAAGAAGCGAAGCACTTTGAAACTCGTTTTGGAACTTTTCTTTGTTGGAGTTATAGAAGATGGTGGTATTTTCGTGAATATCATAAAGGATCTTGCCAGTGGAGAACACTTAAAGGATCGGGATACTACTCAGACAAATTTGACTCTTCTTGCTAGTTTTGCTCGTCAGGGAAGAATGTTTCTTGGCCTTCCACTCGCTGGGCCAGAAATTCATGAAGAGTTTTTTAAGGGCCTTAATATCACGACAGATCAAAAGAAATTCTTCAGGAAGGCATTTCAAACATATTATGCTGCTGCTGCAGAACTGCTGCAATCCGAGCATACTTCACTTCGCCAAATGGAGCATGAAAATGCTAAAATCCTTAATGCTAAAGGAGAGCTCAGTGATGAAAGTGTCTCATCATATGAGAAGCTGCGGAAATCTTATGAACATTTGTACCGCAATGTCTCATCCTTAGCAGAAGCACTTGATATGCAGCCCCCAGTAATGCCAGAGGATGGTCACACAACAAGGGTTACTAGTGGGGAGGATGCTTCATCTCCTGTTGCTGGAAAGGATTCTTCGGCACTCGAAGCCATATGGGATGATGAAGATACAAGGGCTTTCTATGAATGCTTACCAGATCTTAGAGCATTTGTTCCGGCAGTATTATTAGGAGAAGCAGAGTCTAAAAGCAGTGATCAATCTGCAAAGACCCAGGAACAACCAACTGAACCCGCACTTGAATCAGACCAAACTCAACAAATTACAGAAGATACTGGAGAGGCTTCTGCAGATGTTGGTGCTTTACAAGAGGCCAAAAGCACAGAGAAAGGAAAGGACAAGGAAGAAAAAGATAAAGACAAGATCAAAGATCCAGACAAAGAGAAAGTTGACAGAAAAGGAGAGAACGACAAAGAGAAGCTCAAAAGTATTGAAGGGACAAATTTGGATGCTTTACTGCAGAGGCTTCCTGGTTGCGTGAGCCGTGATCTTATTGATCAGTTGACTGTGGAGTTTTGTTATTTAAATTCAAAAGCAAATCGGAAAAAGCTTGTGAGGGCATTATTTAATGTCCCTAGAACATCATTGGAGCTACTAGCTTACTACTCGCGTTTGGTTGCCACACTCTCGACTTGTATGAAGGACGTCTCATCCATGCTCCTAGCGATGGTGGAAGAAGAGTTCAACTTCTTAATTAATAAAAAGGACCAAATGAACATTGAAACGAAGATTAGGAACATTAGATTTATTGGAGAACTCTGCAAGTTTAAGATTGCACCAGCTGGTCTTGTCTTTAGTTGTTTGAAGGCTTGTTTGGATGATTTCAGTCATCATAACATTGATGTTGCTTGCAATCTTCTTGAGACTTGTGGGCGTTTTCTGTATCGGTCTCCTGAAACTACTGTGCGGATGGCTAACATGTTGGAAATATTAATGCGATTGAAGAACGTAAAAAATTTAGACCCTCGGCACAGCACCCTTGTTGAAAATGCTTACTACCTATGCAAACCACCTGAGAGATCTGCACGGGTCACTAAAGTCCGTCCTCCATTGCATCAGTATATAAGAAAGTTGCTATTCTCAGATCTTGATAAGTCTACCATAGAGCATGTGCTCAGGCAACTGCGTAAATTACCATGGGGTGAATGTGAGCCGTATCTTTTAAAATGCTTTCTGAAGGTTCACAAAGGGAAGTATGGTCAGATCCACTTGATTGCTTCTCTCACCGCTGGTTTGAGCCGCTATCATGATGAATTTGCTGTATCTGTTGTTGACGAGGTTCTAGAGGAGATACGGCTTGGCCTAGAATTAAATGAGTATGGGATGCAACAAAGACGGATTGCTCATATGCGATTTTTAGGAGAGCTATACAACTATGAGCATGTAGATTCTTCTGTTATTTTTGAAACTCTGTACTTGGTTCTTGCCTTTGGCCATGGCACACCAGAGCAAGATGTACTGGATCCACCAGAAGATTGTTTCCGCATGAGGATGGTTATTACTCTTCTTGAAACCTGCGGGCACTACTTTGACCGAGGCTCTTCCAAGAGGAAACTTGATCGATTCTTGATGAACTTTCAGAGATATATACTAAGTAAAGGTGTACTACCGCTTGATGTGGAATTTGACTTACAGGACTTATTTGCCGATCTACGCCCCAACATGGCTCGGTACTCTTCCATTGAAGAAGTTAATGCAGCTCTTGTAGAACTTGAGGAGCGTGATCGTACCGTCTCCATTGAGAAGCACTCTGACACTGAAAAGCCTTCAAGGAGGAACACCTCCGACGAAATGTCAGCGAATGGAAAGAGTATTGTAAATGGAACTGGGGAAAATGGTGTAGTTCATGGAAACCTTGGAGATAGTGACAGTGATTCGGGAAGTGGCACCATTGATCGAGATAGACATAATGAAGAAGGATTGGGTGAAGAGAATCATGATGGTGGATCTGACAGTGATGAGGATGATGGTGATGGTGGTGGACCGGCATCCGATGAGGACGATGAAGTCCATGTCAGACAGAAGGTTGCAGAGGTGGACCCTCAAGAAGCAGAAAAGTTTGAGCTGGATCTCAAGGCTGTTATGCAGGAAAGCATGGAGCAACGTAGGCTAGAGCTGAGAGGCCGACCAACATTAAACATGATGATACCGATGAATGTGTTTGAGGGGTCCACCAAGGACCATCATGGACGAGGGGTTGGAGGGGAAAGTGGAGATGAGGCGTTGGATGAAGAGTCTGGAGGAAGCAAGGAGGTCCAGGTGAAAGTTCTTGTCAAGCGTGGTAACAAGCAACAAACAAAGCAGATGTACATCCCTCGGGATTGCTCACTTATCCAGAGTACGAAACAGAAAGAAGCAGAAGAGCTTGAAGAGAAACAAGACATCAAGAGGTTGGTTTTGGAATACAATGACAGAGAAGAGGAGGAGCTTAATGGATTGGGGAACCAAACTCTGAACCATATGCAGAGTGGTGGTCATAGACTTGCCGGCCGTGGCAGCACTTGGGAAGGAGCTAGCGGTAGAGGTGGTGGAGCACGTCATAGATATCACAGTAGTTATTCCGGTGGGGGAGCTTACTACAGCAGGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0031503 GO:0031974 GO:0031981 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034655 GO:0035145 GO:0035690 GO:0035770 GO:0036464 GO:0042162 GO:0042221 GO:0042493 GO:0042742 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046677 GO:0046700 GO:0046907 GO:0048519 GO:0048571 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071426 GO:0071427 GO:0071446 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
MIF4G Upf2

Protein Analysis

1186

Amino Acids

133.65

Weight (kDa)

5.42

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF4G PF02854 63 - 238 2.8e-07 MIF4G domain
MIF4G PF02854 473 - 659 6.5e-41 MIF4G domain
MIF4G PF02854 675 - 870 1.2e-42 MIF4G domain
Upf2 PF04050 987 - 1122 6.1e-31 Up-frameshift suppressor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2969
AatII GACGTC 1 cut(s) 1611
Acc36I ACCTGC 1 cut(s) 2486
AccB1I GGYRCC 1 cut(s) 2849
AccB7I CCANNNNNTGG 2 cut(s) 677, 977
AccBSI CCGCTC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 2060
AccII CGCG 1 cut(s) 1573
AclWI GGATC 9 cut(s) 12, 576, 603, 1328, 2161, 2420, 2433, 2917, 3039
AcoI YGGCCR 2 cut(s) 2395, 3451
AcsI RAATTY 9 cut(s) 281, 616, 687, 737, 1405, 1489, 1918, 2216, 2585
AcuI CTGAAG 3 cut(s) 236, 727, 2159
AcyI GRCGYC 1 cut(s) 1608
AfaI GTAC 9 cut(s) 380, 917, 2378, 2421, 2568, 2636, 2692, 3272, 3304
AfiI CCNNNNNNNGG 7 cut(s) 16, 155, 677, 977, 1028, 1630, 2626
AflIII ACRYGT 1 cut(s) 1881
AhdI GACNNNNNGTC 1 cut(s) 2755
AhlI ACTAGT 1 cut(s) 998
AjiI CACGTC 1 cut(s) 3500
AjnI CCWGG 4 cut(s) 430, 1176, 1432, 3219
AjuI GAANNNNNNNTTGG 4 cut(s) 491, 523, 1760, 1792
AloI GAACNNNNNNTCC 2 cut(s) 1628, 1660
Alw21I GWGCWC 3 cut(s) 861, 2079, 3499
Alw26I GTCTC 6 cut(s) 880, 931, 1615, 1820, 1857, 2701
AlwI GGATC 9 cut(s) 12, 576, 603, 1328, 2161, 2420, 2433, 2917, 3039
Ama87I CYCGRG 2 cut(s) 140, 3279
ApoI RAATTY 9 cut(s) 281, 616, 687, 737, 1405, 1489, 1918, 2216, 2585
AseI ATTAAT 2 cut(s) 1659, 1895
Asp700I GAANNNNTTC 3 cut(s) 422, 740, 1036
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 9 cut(s) 474, 680, 709, 1669, 2945, 3001, 3132, 3142, 3217
AsuHPI GGTGA 5 cut(s) 2117, 2179, 2900, 2945, 3235
AsuNHI GCTAGC 1 cut(s) 3476
AvaI CYCGRG 2 cut(s) 140, 3279
AvaII GGWCC 6 cut(s) 1669, 2945, 3001, 3132, 3142, 3217
AxyI CCTNAGG 2 cut(s) 164, 470
BaeGI GKGCMC 1 cut(s) 2487
BaeI ACNNNNGTAYC 2 cut(s) 899, 932
BalI TGGCCA 1 cut(s) 2397
BamHI GGATCC 1 cut(s) 2425
BanI GGYRCC 1 cut(s) 2849
BanII GRGCYC 1 cut(s) 861
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
BbsI GAAGAC 1 cut(s) 415
Bbv12I GWGCWC 3 cut(s) 861, 2079, 3499
BceAI ACGGC 5 cut(s) 9, 1431, 2101, 2273, 3438
BciT130I CCWGG 4 cut(s) 432, 1178, 1434, 3221
BciVI GTATCC 1 cut(s) 595
BclI TGATCA 2 cut(s) 1159, 1459
BcoDI GTCTC 6 cut(s) 880, 931, 1615, 1820, 1857, 2701
BcuI ACTAGT 1 cut(s) 998
BfmI CTRYAG 4 cut(s) 777, 1251, 1421, 3541
BfuAI ACCTGC 1 cut(s) 2486
BfuI GTATCC 1 cut(s) 595
BglI GCCNNNNNGGC 1 cut(s) 30
BglII AGATCT 4 cut(s) 354, 1102, 1982, 2047
Bme1390I CCNGG 4 cut(s) 432, 1178, 1434, 3221
Bme18I GGWCC 6 cut(s) 1669, 2945, 3001, 3132, 3142, 3217
BmeRI GACNNNNNGTC 1 cut(s) 2755
BmeT110I CYCGRG 2 cut(s) 140, 3279
BmgBI CACGTC 1 cut(s) 3500
BmgT120I GGNCC 9 cut(s) 474, 680, 709, 1669, 2945, 3001, 3132, 3142, 3217
BmiI GGNNCC 5 cut(s) 2427, 2851, 3003, 3133, 3407
BmrFI CCNGG 4 cut(s) 432, 1178, 1434, 3221
BmrI ACTGGG 2 cut(s) 956, 2799
BmsI GCATC 6 cut(s) 304, 1000, 1402, 2026, 2277, 2960
BmtI GCTAGC 1 cut(s) 3480
BmuI ACTGGG 2 cut(s) 956, 2799
BpiI GAAGAC 1 cut(s) 415
BplI GAGNNNNNCTC 2 cut(s) 2696, 2728
BpmI CTGGAG 2 cut(s) 1260, 3222
Bpu10I CCTNAGC 3 cut(s) 275, 934, 2078
BpuEI CTTGAG 6 cut(s) 101, 280, 1844, 2696, 2991, 3020
Bsa29I ATCGAT 1 cut(s) 2524
BsaBI GATNNNNATC 1 cut(s) 3272
BsaHI GRCGYC 1 cut(s) 1608
BsaI GGTCTC 1 cut(s) 1857
BsaWI WCCGGW 1 cut(s) 3524
Bsc4I CCNNNNNNNGG 7 cut(s) 16, 155, 677, 977, 1028, 1630, 2626
Bse118I RCCGGY 2 cut(s) 2947, 3449
Bse1I ACTGG 6 cut(s) 177, 576, 962, 1243, 2427, 2794
Bse21I CCTNAGG 2 cut(s) 164, 470
Bse3DI GCAATG 3 cut(s) 40, 928, 2012
Bse8I GATNNNNATC 1 cut(s) 3272
BseBI CCWGG 4 cut(s) 432, 1178, 1434, 3221
BseCI ATCGAT 1 cut(s) 2524
BseJI GATNNNNATC 1 cut(s) 3272
BseLI CCNNNNNNNGG 7 cut(s) 16, 155, 677, 977, 1028, 1630, 2626
BseMI GCAATG 3 cut(s) 40, 928, 2012
BseMII CTCAG 8 cut(s) 266, 461, 623, 874, 1968, 2058, 2092, 3071
BseNI ACTGG 6 cut(s) 177, 576, 962, 1243, 2427, 2794
BseRI GAGGAG 5 cut(s) 29, 2264, 2693, 3398, 3401
BseSI GKGCMC 1 cut(s) 2487
BseX3I CGGCCG 1 cut(s) 3451
BseYI CCCAGC 1 cut(s) 677
BsgI GTGCAG 1 cut(s) 1971
Bsh1236I CGCG 1 cut(s) 1573
Bsh1285I CGRYCG 1 cut(s) 3454
BshNI GGYRCC 1 cut(s) 2849
BshVI ATCGAT 1 cut(s) 2524
BsiEI CGRYCG 1 cut(s) 3454
BsiHKAI GWGCWC 3 cut(s) 861, 2079, 3499
BsiHKCI CYCGRG 2 cut(s) 140, 3279
BsiSI CCGG 4 cut(s) 1121, 2948, 3450, 3525
BslFI GGGAC 2 cut(s) 1414, 1520
BslI CCNNNNNNNGG 7 cut(s) 16, 155, 677, 977, 1028, 1630, 2626
BsmAI GTCTC 6 cut(s) 880, 931, 1615, 1820, 1857, 2701
BsmBI CGTCTC 2 cut(s) 1615, 2701
BsmFI GGGAC 2 cut(s) 1414, 1520
BsmI GAATGC 2 cut(s) 26, 1097
Bso31I GGTCTC 1 cut(s) 1857
BsoBI CYCGRG 2 cut(s) 140, 3279
Bsp1286I GDGCHC 4 cut(s) 861, 2079, 2487, 3499
Bsp1407I TGTACA 1 cut(s) 3270
Bsp19I CCATGG 2 cut(s) 2099, 2398
BspCNI CTCAG 8 cut(s) 267, 462, 622, 873, 1969, 2057, 2091, 3072
BspDI ATCGAT 1 cut(s) 2524
BspFNI CGCG 1 cut(s) 1573
BspHI TCATGA 3 cut(s) 691, 2209, 2899
BspLI GGNNCC 5 cut(s) 2427, 2851, 3003, 3133, 3407
BspMAI CTGCAG 3 cut(s) 781, 1255, 1425
BspMI ACCTGC 1 cut(s) 2486
BspOI GCTAGC 1 cut(s) 3480
BspPI GGATC 9 cut(s) 12, 576, 603, 1328, 2161, 2420, 2433, 2917, 3039
BspQI GCTCTTC 4 cut(s) 123, 225, 2509, 3318
BspT107I GGYRCC 1 cut(s) 2849
BspTNI GGTCTC 1 cut(s) 1857
BsrBI CCGCTC 1 cut(s) 145
BsrDI GCAATG 3 cut(s) 40, 928, 2012
BsrFI RCCGGY 2 cut(s) 2947, 3449
BsrGI TGTACA 1 cut(s) 3270
BsrI ACTGG 6 cut(s) 177, 576, 962, 1243, 2427, 2794
BssAI RCCGGY 2 cut(s) 2947, 3449
BssNI GRCGYC 1 cut(s) 1608
BssT1I CCWWGG 4 cut(s) 2099, 2398, 2818, 3138
Bst2UI CCWGG 4 cut(s) 432, 1178, 1434, 3221
Bst4CI ACNGT 6 cut(s) 1471, 1867, 2695, 2834, 2918, 3515
BstACI GRCGYC 1 cut(s) 1608
BstAUI TGTACA 1 cut(s) 3270
BstC8I GCNNGC 5 cut(s) 31, 1813, 2483, 3451, 3478
BstDSI CCRYGG 3 cut(s) 2099, 2398, 3454
BstENI CCTNNNNNAGG 1 cut(s) 153
BstFNI CGCG 1 cut(s) 1573
BstHHI GCGC 1 cut(s) 192
BstMAI GTCTC 6 cut(s) 880, 931, 1615, 1820, 1857, 2701
BstMCI CGRYCG 1 cut(s) 3454
BstMWI GCNNNNNNNGC 9 cut(s) 30, 189, 302, 638, 776, 796, 1052, 3070, 3459
BstNI CCWGG 4 cut(s) 432, 1178, 1434, 3221
BstNSI RCATGY 3 cut(s) 1885, 2075, 2348
BstSCI CCNGG 4 cut(s) 430, 1176, 1432, 3219
BstSFI CTRYAG 4 cut(s) 777, 1251, 1421, 3541
BstSLI GKGCMC 1 cut(s) 2487
BstUI CGCG 1 cut(s) 1573
BstV2I GAAGAC 1 cut(s) 415
BstZI CGGCCG 1 cut(s) 3451
Bsu15I ATCGAT 1 cut(s) 2524
Bsu36I CCTNAGG 2 cut(s) 164, 470
BsuI GTATCC 1 cut(s) 595
BsuTUI ATCGAT 1 cut(s) 2524
BtgI CCRYGG 3 cut(s) 2099, 2398, 3454
BtgZI GCGATG 1 cut(s) 1643
BtrI CACGTC 1 cut(s) 3500
BtsI GCAGTG 1 cut(s) 1162
BtsIMutI CAGTG 8 cut(s) 170, 295, 583, 868, 1162, 2718, 2839, 2923
BveI ACCTGC 1 cut(s) 2486
Cac8I GCNNGC 5 cut(s) 31, 1813, 2483, 3451, 3478
CciI TCATGA 3 cut(s) 691, 2209, 2899
CfoI GCGC 1 cut(s) 192
Cfr10I RCCGGY 2 cut(s) 2947, 3449
Cfr13I GGNCC 9 cut(s) 474, 680, 709, 1669, 2945, 3001, 3132, 3142, 3217
ClaI ATCGAT 1 cut(s) 2524
Csp6I GTAC 9 cut(s) 379, 916, 2377, 2420, 2567, 2635, 2691, 3271, 3303
CviQI GTAC 9 cut(s) 379, 916, 2377, 2420, 2567, 2635, 2691, 3271, 3303
DraI TTTAAA 2 cut(s) 1488, 2127
DrdI GACNNNNNNGTC 1 cut(s) 2969
DriI GACNNNNNGTC 1 cut(s) 2755
DseDI GACNNNNNNGTC 1 cut(s) 2969
EaeI YGGCCR 2 cut(s) 2395, 3451
EagI CGGCCG 1 cut(s) 3451
Eam1105I GACNNNNNGTC 1 cut(s) 2755
Ecl136II GAGCTC 1 cut(s) 859
EclXI CGGCCG 1 cut(s) 3451
Eco130I CCWWGG 4 cut(s) 2099, 2398, 2818, 3138
Eco24I GRGCYC 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 1857
Eco32I GATATC 1 cut(s) 3509
Eco47I GGWCC 6 cut(s) 1669, 2945, 3001, 3132, 3142, 3217
Eco52I CGGCCG 1 cut(s) 3451
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 3 cut(s) 236, 727, 2159
Eco81I CCTNAGG 2 cut(s) 164, 470
Eco88I CYCGRG 2 cut(s) 140, 3279
EcoICRI GAGCTC 1 cut(s) 859
EcoNI CCTNNNNNAGG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 709
EcoRII CCWGG 4 cut(s) 430, 1176, 1432, 3219
EcoRV GATATC 1 cut(s) 3509
EcoT14I CCWWGG 4 cut(s) 2099, 2398, 2818, 3138
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 4 cut(s) 2099, 2398, 2818, 3138
Esp3I CGTCTC 2 cut(s) 1615, 2701
FalI AAGNNNNNCTT 2 cut(s) 725, 757
FaqI GGGAC 2 cut(s) 1414, 1520
FauI CCCGC 3 cut(s) 231, 1204, 2474
FauNDI CATATG 4 cut(s) 883, 1058, 2310, 3423
FbaI TGATCA 2 cut(s) 1159, 1459
FblI GTMKAC 1 cut(s) 2060
FriOI GRGCYC 1 cut(s) 861
GlaI GCGC 1 cut(s) 191
GsaI CCCAGC 1 cut(s) 681
GsuI CTGGAG 2 cut(s) 1260, 3222
HapII CCGG 4 cut(s) 1121, 2948, 3450, 3525
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 1608
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HincII GTYRAC 4 cut(s) 271, 1354, 1467, 2236
HindII GTYRAC 4 cut(s) 271, 1354, 1467, 2236
HindIII AAGCTT 2 cut(s) 320, 1511
HpaII CCGG 4 cut(s) 1121, 2948, 3450, 3525
HphI GGTGA 5 cut(s) 2117, 2179, 2900, 2945, 3235
Hpy99I CGWCG 1 cut(s) 2753
HpyCH4III ACNGT 6 cut(s) 1471, 1867, 2695, 2834, 2918, 3515
HpyCH4IV ACGT 4 cut(s) 1608, 1911, 3067, 3499
HpyF10VI GCNNNNNNNGC 9 cut(s) 30, 189, 302, 638, 776, 796, 1052, 3070, 3459
HpySE526I ACGT 4 cut(s) 1608, 1911, 3067, 3499
Hsp92I GRCGYC 1 cut(s) 1608
HspAI GCGC 1 cut(s) 190
KroI GCCGGC 1 cut(s) 3449
KroNI GCCGGC 1 cut(s) 3451
Ksp22I TGATCA 2 cut(s) 1159, 1459
LguI GCTCTTC 4 cut(s) 123, 225, 2509, 3318
LweI GCATC 6 cut(s) 304, 1000, 1402, 2026, 2277, 2960
MaeII ACGT 4 cut(s) 1608, 1911, 3067, 3499
MaeIII GTNAC 6 cut(s) 298, 980, 994, 1993, 2828, 3245
MbiI CCGCTC 1 cut(s) 145
MhlI GDGCHC 4 cut(s) 861, 2079, 2487, 3499
MlsI TGGCCA 1 cut(s) 2397
MluNI TGGCCA 1 cut(s) 2397
MlyI GAGTC 3 cut(s) 616, 1154, 3206
MmeI TCCRAC 5 cut(s) 505, 1866, 2772, 3142, 3168
Mox20I TGGCCA 1 cut(s) 2397
MroNI GCCGGC 1 cut(s) 3449
MroXI GAANNNNTTC 3 cut(s) 422, 740, 1036
MscI TGGCCA 1 cut(s) 2397
MslI CAYNNNNRTG 3 cut(s) 828, 2453, 2904
Msp20I TGGCCA 1 cut(s) 2397
MspA1I CMGCKG 4 cut(s) 200, 238, 1745, 2192
MspI CCGG 4 cut(s) 1121, 2948, 3450, 3525
MspR9I CCNGG 4 cut(s) 432, 1178, 1434, 3221
Mva1269I GAATGC 2 cut(s) 26, 1097
MvaI CCWGG 4 cut(s) 432, 1178, 1434, 3221
MvnI CGCG 1 cut(s) 1573
MwoI GCNNNNNNNGC 9 cut(s) 30, 189, 302, 638, 776, 796, 1052, 3070, 3459
NaeI GCCGGC 1 cut(s) 3451
NcoI CCATGG 2 cut(s) 2099, 2398
NdeI CATATG 4 cut(s) 883, 1058, 2310, 3423
NgoMIV GCCGGC 1 cut(s) 3449
NheI GCTAGC 1 cut(s) 3476
NlaIV GGNNCC 5 cut(s) 2427, 2851, 3003, 3133, 3407
NmeAIII GCCGAG 2 cut(s) 55, 1909
NmuCI GTSAC 3 cut(s) 980, 1993, 2828
NspI RCATGY 3 cut(s) 1885, 2075, 2348
PacI TTAATTAA 1 cut(s) 1659
PagI TCATGA 3 cut(s) 691, 2209, 2899
PciI ACATGT 1 cut(s) 1881
PciSI GCTCTTC 4 cut(s) 123, 225, 2509, 3318
PctI GAATGC 2 cut(s) 26, 1097
PdiI GCCGGC 1 cut(s) 3451
PdmI GAANNNNTTC 3 cut(s) 422, 740, 1036
PfeI GAWTC 8 cut(s) 159, 176, 1037, 1205, 2351, 2526, 2837, 2896
PflMI CCANNNNNTGG 2 cut(s) 677, 977
PleI GAGTC 3 cut(s) 616, 1153, 3205
PpsI GAGTC 3 cut(s) 616, 1153, 3205
PscI ACATGT 1 cut(s) 1881
PshBI ATTAAT 2 cut(s) 1659, 1895
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 4 cut(s) 430, 1176, 1432, 3219
PspFI CCCAGC 1 cut(s) 677
PspGI CCWGG 4 cut(s) 430, 1176, 1432, 3219
PspN4I GGNNCC 5 cut(s) 2427, 2851, 3003, 3133, 3407
PspPI GGNCC 9 cut(s) 474, 680, 709, 1669, 2945, 3001, 3132, 3142, 3217
PstI CTGCAG 3 cut(s) 781, 1255, 1425
PvuII CAGCTG 1 cut(s) 1745
RsaI GTAC 9 cut(s) 380, 917, 2378, 2421, 2568, 2636, 2692, 3272, 3304
RsaNI GTAC 9 cut(s) 379, 916, 2377, 2420, 2567, 2635, 2691, 3271, 3303
RseI CAYNNNNRTG 3 cut(s) 828, 2453, 2904
SacI GAGCTC 1 cut(s) 861
SapI GCTCTTC 4 cut(s) 123, 225, 2509, 3318
Sau96I GGNCC 9 cut(s) 474, 680, 709, 1669, 2945, 3001, 3132, 3142, 3217
SchI GAGTC 3 cut(s) 616, 1154, 3206
ScrFI CCNGG 4 cut(s) 432, 1178, 1434, 3221
SduI GDGCHC 4 cut(s) 861, 2079, 2487, 3499
SfaNI GCATC 6 cut(s) 304, 1000, 1402, 2026, 2277, 2960
SfcI CTRYAG 4 cut(s) 777, 1251, 1421, 3541
SinI GGWCC 6 cut(s) 1669, 2945, 3001, 3132, 3142, 3217
SmiI ATTTAAAT 1 cut(s) 1488
SmiMI CAYNNNNRTG 3 cut(s) 828, 2453, 2904
SmlI CTYRAG 6 cut(s) 80, 259, 1823, 2675, 3006, 3035
SmoI CTYRAG 6 cut(s) 80, 259, 1823, 2675, 3006, 3035
SpeI ACTAGT 1 cut(s) 998
SspI AATATT 1 cut(s) 1893
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 4 cut(s) 430, 1176, 1432, 3219
StyI CCWWGG 4 cut(s) 2099, 2398, 2818, 3138
SwaI ATTTAAAT 1 cut(s) 1488
TaaI ACNGT 6 cut(s) 1471, 1867, 2695, 2834, 2918, 3515
TaiI ACGT 4 cut(s) 1611, 1914, 3070, 3502
TaqI TCGA 4 cut(s) 1050, 1592, 2524, 2860
TaqII GACCGA 2 cut(s) 1839, 2511
TatI WGTACW 4 cut(s) 2376, 2419, 2566, 3270
TauI GCSGC 2 cut(s) 148, 2205
TfiI GAWTC 8 cut(s) 159, 176, 1037, 1205, 2351, 2526, 2837, 2896
TscAI CASTG 8 cut(s) 177, 295, 583, 868, 1162, 2725, 2839, 2923
TseFI GTSAC 3 cut(s) 980, 1993, 2828
Tsp45I GTSAC 3 cut(s) 980, 1993, 2828
TspGWI ACGGA 2 cut(s) 1994, 2314
TspRI CASTG 8 cut(s) 177, 295, 583, 868, 1162, 2725, 2839, 2923
Van91I CCANNNNNTGG 2 cut(s) 677, 977
VpaK11BI GGWCC 6 cut(s) 1669, 2945, 3001, 3132, 3142, 3217
VspI ATTAAT 2 cut(s) 1659, 1895
XagI CCTNNNNNAGG 1 cut(s) 153
XapI RAATTY 9 cut(s) 281, 616, 687, 737, 1405, 1489, 1918, 2216, 2585
XbaI TCTAGA 1 cut(s) 2245
XceI RCATGY 3 cut(s) 1885, 2075, 2348
XcmI CCANNNNNNNNNTGG 1 cut(s) 3428
XmiI GTMKAC 1 cut(s) 2060
XmnI GAANNNNTTC 3 cut(s) 422, 740, 1036
ZraI GACGTC 1 cut(s) 1609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.