MD01G1113500.v1.1

Regulator of nonsense transcripts

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007975
Physical Location & Seq
Reverse (-)
22757365 .. 22769681
12317 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1113500.v1.1.491

Sequence Viewer

Length: 3579 bp
ATGGAGCACCATGAGGAGGAAGGCGGTGCTGGCAGTGAACCTCATGGGAAGCAAGATGATGAGGAAGCTGCTGCTCGGCTTGAGGAAGTCAAGAAATCTATTGAGGCAAAGATGGCTCTTCGTCAAAGCAATCTGAACCCTGAGCGGCCTGACACAGGATTTCTGAGGACATTGGATTCTAGCATTAAGCGCAACACAGCGGTTATTAAAAAACTGAAGCAGATAAATGAAGAGCAGCGGGAAGGTTTGATGGATGACTTGAGAGGTGTCAACCTAAGCAAATTTGTCAGTGAAGCTGTTACTGCTATTTGTGATGCCAAGCTTAGATCGTCAGACATACAAGCAGCAGTCCAGATCTGCTCTCTGCTTCATCAAAGGTACAAAGACTTCTCCCCAACTCTGCTTCAAGGTCTTTTGAAAGTATTTTTTCCGGGAAAGTCTGGGGATGACTTAGAAGCAGACAAGAACTTGAGGGCCATGAAGAAGCGAAGCACTTTGAAACTCCTTGTGGAACTTTTCTTTGTTGGAGTTATAGAAGACGGTGGTGTTTTCTTGAATATCATTAAGGATCTTACCAGTGGAGAACACTTAAAGGATCGGGATACTACTCAGACAAATTTGACTCTTCTTGCTAGTTTTGCTCGTCAGGGAAGAATGTTTCTTGGCCTTCCACTCTCTGGGCCGGAAATTCATGAAGAGTTTTTTAAGGGCCTTAGTATCACGACAGATCAAAAGAAATTCTTCAGGAAGGCATTTCAAACATATTATGTTGCTGCTGCAGAACTGTTGCAATCCGAGCATACTTCACTTCGCCAAATGGAGCATGAAAATGCTAAAATCCTTAATGCTAAAGGAGAGCTCAGTGATGAAAGTGTCTCGTCATACGAGAAGCTGCGGAAATCATATGAACATTTGTACCGCAATGTCTCATCTTTAGCAGAAGCACTTGATATGCAGCCCCCAGTAATGCCAGAGGATGGTCACACAACAAGGGTTACTAGTGGGGAGGATGCTTCATCCGCTGCTGCTGGAAAGGATTCTTCGGCACTTGAAGCCATATGGGATGATGAAGATACAAGGGCTTTCTACGAATGCTTACCAGATCTTAGAGCATTTGTTCCGGCAGTATTATTGGGAGAAGCAGAGTCTAAAAGCAGTGATCAATCTGCAAAGACCCAGGAACAATCAACTGAACCCGCACTTGAATCAGACCAAAGTCAACAAACTACAGAAGATACTGGAGAGGCTTCTGCAGATGTTGGTGCTTTACAAGAGGGCAAAACCATAGAGAAAGGAAAGGACAAGGAAGAAAAAGAAAAAGACAAGATCAAAGATCCAGACAAAGAGAAAGGTGATAGAAAAGGAGAGCATGAGAAAGAGAAGCTCAAAAGTATTGAAGGGACAAATTTGGATGCTTTACTGCAGAGGCTTCCTGGTTGCGTGAGCCGTGATCTTATTGATCAGTTGACTGTAGAGTTTTGTTATTTAAATTCAAAAGCAAATCGGAAAAAGCTTGTGAGGGCAGTATTTAATGTCCCTAGAACCTCATTGGAGCTACTAGCTTACTATTCGCGTTTGGTTGCCACACTCTCAACTTGTATGAAGGACGTCTCATCCATGCTCCTAGCAATGTTGGAAGAAGAGTTCAACTTCTTAATTAATAAAAAGGATCAAATGAACATTGAAACAAAGATTAGGAACATTAGATTTATTGGAGAACTCTGCAAGTTTAAGATTGCACCAGCTGGTCTTGTCTTTAGTTGTTTGAAGGCTTGTTTGGATGATTTCAGTCATCATAACATTGATGTTGCTTGCAATCTTCTTGAGACTTGTGGGCGCTTTCTGTATCGATCTCCTGAAACTACTGTGCGGATGGCAAACATGTTGGAAATATTAATGCGCTTGAAGAATGTAAAAAATTTAGACCCTCGGCACAGCACCCTTGTTGAAAATGCCTACTACCTATGCAAACCACCTGAGAGATCCGCACGGGTCACTAAAGTCCGTCCTCCATTGCATCAGTATATAAGAAAGTTGCTCTTCTCAGATCTTGACAAGTCTACCATAGAGCATGTGCTCAGGCAACTGCGTAAACTACCATGGGGTGAATGTGAGCCGTATCTTTTAAAATGCTTTCTGAAGGTTCACAAGGGGAAGTATGGTCAGATCCACTTGATTGCTTCTCTCACCGCTGGTTTGAGCCGCTATCATGATGAATTTGCTGTATCTGTTGTTGACGAGGTTCTAGAGGAGATACGGCTTGGCCTAGAATTAAATGAGTATGGGATGCAGCAAAGACGGATTGCCCATATGCGATTCTTAGGAGAGCTATACAACTATGAGCATGTAGATTCTTCTGTTATTTTTGAGACTCTGTATTTGACTCTTGCCTTTGGCCACGGCACACCAGAGCAAGATGTACTAGATCCACCAGAAGATTGTTTCCGCATGAGGATGGTCATTACTCTTCTTGAAACCTGTGGTCACTACTTTGATCGAGGCTCTTCCAAGAGGAAACTTGATCGATTCTTGATGCACTTTCAGAGATATATACTAAGTAAAGGTGTACTACCGCTTGATGTTGAATTTGACTTACAGGACTTGTTTGCCGATCTACGCCCCAACATGACTCGATACTCTTCCATTGAAGAAGTTAATGCAGCTCTTGTAGAACTTGAGGAGCGCGATCATACTGTCTCCATTGACAAGAACAATAATGAGAAGCACTCCGACACTGAAAAACCTTCAAGGAGGAACACCTCCAACAAAATGTCAGTGAATGGAAAGAGTGTTGTGAATGGAACCGAGGAAAATGGTGTTGATCATGGAGACCTTGGAGATAGTGACAGTGATTCGGGAGGTGGCACCATTAATCGAGATAGACATGATGAAGAAGGATTGGGTGAAGAGAATCATGATGGTGGATCTGACAGTAATGAGGATGATGATGATGATGGTGGTGGAGGACCGGTATCCGATGAGGACGATGAAGTCCATGTCAGACAGAAGGTTGCAGAAGTGGACCCTCAAGAAGCAGAAAAGTTTGAGCTGGATCTCAAAGCTGTTATGCAGGAAAGCATGGAGCAACGTAGGCTAGAGCTGAGAAGCCGACCAACATTAAACATGACAATACCGATGAATGTGTTTGAGGGGTCCACCAAGGACCATCATGGAAGAGTGGTTGGAGGGGAAAGTGGAGATGAGGCGTTGGATGAAGAGTCTGGAGGAAGCAAGGAGGTCCAGGTGAAAGTTCTTGTCAAGCGTGGTAACAAGCAACAAACGAAGCAGATGTACATCCCTCGGGATTGCTCACTTATCCAGAGTACGAAACAGAAAGAAGCAGCAGAGCTTGAAGAGAAACAAGACATCAAGAGGTTGGTTTTGGAATACAATGACAGAGAAGAAGAGGAGCTTAATGGATTGGGGAACCAAACTCTGAACTATATGCAGAGTGGTGGCAATAGACTTGCAGGCCGTGGCAGCACTTGGGAAGGAACTAGCGGTAGAGGTGGTGGAGCACGTCATAGATATCACAGTAGTTATTCCGGCGGGGGAGTTTACTACAGCAGGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0031503 GO:0031974 GO:0031981 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034655 GO:0035145 GO:0035690 GO:0035770 GO:0036464 GO:0042162 GO:0042221 GO:0042493 GO:0042742 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046677 GO:0046700 GO:0046907 GO:0048519 GO:0048571 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071426 GO:0071427 GO:0071446 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
MIF4G Upf2

Protein Analysis

1193

Amino Acids

134.39

Weight (kDa)

5.39

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF4G PF02854 63 - 224 1.2e-08 MIF4G domain
MIF4G PF02854 473 - 659 1.2e-40 MIF4G domain
MIF4G PF02854 675 - 870 1.4e-42 MIF4G domain
Upf2 PF04050 994 - 1129 5.2e-31 Up-frameshift suppressor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2990
AatII GACGTC 1 cut(s) 1611
AccB1I GGYRCC 1 cut(s) 2864
AccB7I CCANNNNNTGG 2 cut(s) 677, 977
AccBSI CCGCTC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 2060
AccII CGCG 2 cut(s) 1573, 2685
AclWI GGATC 9 cut(s) 576, 603, 1328, 1677, 1977, 2161, 2420, 2932, 3060
AcoI YGGCCR 1 cut(s) 2395
AcsI RAATTY 9 cut(s) 281, 616, 687, 737, 1405, 1489, 1918, 2216, 2585
AcuI CTGAAG 3 cut(s) 236, 727, 2159
AcyI GRCGYC 1 cut(s) 1608
AfaI GTAC 6 cut(s) 380, 917, 2421, 2568, 3293, 3325
AfiI CCNNNNNNNGG 4 cut(s) 16, 155, 677, 977
AflIII ACRYGT 1 cut(s) 1881
AgeI ACCGGT 1 cut(s) 2968
AhlI ACTAGT 1 cut(s) 998
AjiI CACGTC 1 cut(s) 3521
AjnI CCWGG 3 cut(s) 1176, 1432, 3240
AjuI GAANNNNNNNTTGG 2 cut(s) 1760, 1792
AloI GAACNNNNNNTCC 2 cut(s) 1628, 1660
Alw21I GWGCWC 4 cut(s) 9, 861, 2079, 3520
Alw26I GTCTC 7 cut(s) 880, 931, 1615, 1820, 2363, 2701, 2823
AlwI GGATC 9 cut(s) 576, 603, 1328, 1677, 1977, 2161, 2420, 2932, 3060
Ama87I CYCGRG 1 cut(s) 3300
AoxI GGCC 8 cut(s) 146, 474, 664, 680, 709, 2263, 2395, 3472
ApoI RAATTY 9 cut(s) 281, 616, 687, 737, 1405, 1489, 1918, 2216, 2585
ArsI GACNNNNNNTTYG 2 cut(s) 2362, 2394
AseI ATTAAT 3 cut(s) 1659, 1895, 2871
AsiGI ACCGGT 1 cut(s) 2968
Asp700I GAANNNNTTC 2 cut(s) 740, 1036
AspLEI GCGC 4 cut(s) 192, 1839, 1902, 2685
AspS9I GGNCC 8 cut(s) 474, 680, 709, 2966, 3022, 3153, 3163, 3238
AsuC2I CCSGG 1 cut(s) 432
AsuHPI GGTGA 5 cut(s) 1364, 2117, 2179, 2915, 3256
AvaI CYCGRG 1 cut(s) 3300
AvaII GGWCC 5 cut(s) 2966, 3022, 3153, 3163, 3238
BaeI ACNNNNGTAYC 2 cut(s) 899, 932
BalI TGGCCA 1 cut(s) 2397
BanI GGYRCC 1 cut(s) 2864
BanII GRGCYC 1 cut(s) 861
BarI GAAGNNNNNNTAC 4 cut(s) 371, 403, 3275, 3307
BbsI GAAGAC 1 cut(s) 543
Bbv12I GWGCWC 4 cut(s) 9, 861, 2079, 3520
BccI CCATC 8 cut(s) 106, 244, 971, 1867, 2449, 2912, 2948, 3174
BceAI ACGGC 5 cut(s) 1431, 2101, 2273, 2416, 3459
BciT130I CCWGG 3 cut(s) 1178, 1434, 3242
BciVI GTATCC 2 cut(s) 595, 2983
BclI TGATCA 3 cut(s) 1159, 1459, 2821
BcnI CCSGG 1 cut(s) 432
BcoDI GTCTC 7 cut(s) 880, 931, 1615, 1820, 2363, 2701, 2823
BcuI ACTAGT 1 cut(s) 998
BfmI CTRYAG 6 cut(s) 777, 1227, 1251, 1421, 1470, 3562
BfoI RGCGCY 1 cut(s) 1840
BfuI GTATCC 2 cut(s) 595, 2983
BglII AGATCT 3 cut(s) 354, 1102, 2047
Bme1390I CCNGG 4 cut(s) 432, 1178, 1434, 3242
Bme18I GGWCC 5 cut(s) 2966, 3022, 3153, 3163, 3238
BmeT110I CYCGRG 1 cut(s) 3300
BmgBI CACGTC 1 cut(s) 3521
BmgT120I GGNCC 8 cut(s) 474, 680, 709, 2966, 3022, 3153, 3163, 3238
BmiI GGNNCC 5 cut(s) 2803, 2866, 3024, 3154, 3428
BmrFI CCNGG 4 cut(s) 432, 1178, 1434, 3242
BmrI ACTGGG 1 cut(s) 956
BmsI GCATC 6 cut(s) 304, 1000, 1402, 2026, 2277, 2523
BmuI ACTGGG 1 cut(s) 956
BoxI GACNNNNGTC 1 cut(s) 1215
BpiI GAAGAC 1 cut(s) 543
BplI GAGNNNNNCTC 2 cut(s) 2711, 2743
BpmI CTGGAG 2 cut(s) 1260, 3243
Bpu10I CCTNAGC 3 cut(s) 141, 275, 2078
BpuEI CTTGAG 6 cut(s) 101, 280, 490, 1844, 2696, 3012
BpuMI CCSGG 1 cut(s) 432
Bsa29I ATCGAT 2 cut(s) 1849, 2524
BsaBI GATNNNNATC 1 cut(s) 3293
BsaHI GRCGYC 1 cut(s) 1608
BsaI GGTCTC 1 cut(s) 2823
BsaJI CCNNGG 9 cut(s) 1176, 1928, 2099, 2398, 2805, 2833, 3159, 3299, 3475
BsaWI WCCGGW 1 cut(s) 2968
BsaXI ACNNNNNCTCC 2 cut(s) 2955, 2985
Bsc4I CCNNNNNNNGG 4 cut(s) 16, 155, 677, 977
Bse118I RCCGGY 1 cut(s) 2968
Bse1I ACTGG 3 cut(s) 576, 962, 1243
Bse3DI GCAATG 3 cut(s) 928, 1635, 2012
Bse8I GATNNNNATC 1 cut(s) 3293
BseBI CCWGG 3 cut(s) 1178, 1434, 3242
BseCI ATCGAT 2 cut(s) 1849, 2524
BseDI CCNNGG 9 cut(s) 1176, 1928, 2099, 2398, 2805, 2833, 3159, 3299, 3475
BseJI GATNNNNATC 1 cut(s) 3293
BseLI CCNNNNNNNGG 4 cut(s) 16, 155, 677, 977
BseMI GCAATG 3 cut(s) 928, 1635, 2012
BseMII CTCAG 8 cut(s) 132, 155, 623, 874, 1968, 2058, 2092, 3092
BseNI ACTGG 3 cut(s) 576, 962, 1243
BseRI GAGGAG 4 cut(s) 29, 2264, 2693, 3422
Bsh1236I CGCG 2 cut(s) 1573, 2685
BshFI GGCC 8 cut(s) 148, 476, 666, 682, 711, 2265, 2397, 3474
BshNI GGYRCC 1 cut(s) 2864
BshTI ACCGGT 1 cut(s) 2968
BshVI ATCGAT 2 cut(s) 1849, 2524
BsiHKAI GWGCWC 4 cut(s) 9, 861, 2079, 3520
BsiHKCI CYCGRG 1 cut(s) 3300
BsiSI CCGG 5 cut(s) 431, 683, 1121, 2969, 3546
BslFI GGGAC 2 cut(s) 1414, 1520
BslI CCNNNNNNNGG 4 cut(s) 16, 155, 677, 977
BsmAI GTCTC 7 cut(s) 880, 931, 1615, 1820, 2363, 2701, 2823
BsmBI CGTCTC 1 cut(s) 1615
BsmFI GGGAC 2 cut(s) 1414, 1520
BsmI GAATGC 1 cut(s) 1097
BsnI GGCC 8 cut(s) 148, 476, 666, 682, 711, 2265, 2397, 3474
Bso31I GGTCTC 1 cut(s) 2823
BsoBI CYCGRG 1 cut(s) 3300
Bsp1286I GDGCHC 4 cut(s) 9, 861, 2079, 3520
Bsp1407I TGTACA 1 cut(s) 3291
Bsp19I CCATGG 1 cut(s) 2099
BspANI GGCC 8 cut(s) 148, 476, 666, 682, 711, 2265, 2397, 3474
BspCNI CTCAG 8 cut(s) 133, 156, 622, 873, 1969, 2057, 2091, 3093
BspDI ATCGAT 2 cut(s) 1849, 2524
BspFNI CGCG 2 cut(s) 1573, 2685
BspHI TCATGA 3 cut(s) 691, 2209, 2914
BspLI GGNNCC 5 cut(s) 2803, 2866, 3024, 3154, 3428
BspMAI CTGCAG 3 cut(s) 781, 1255, 1425
BspPI GGATC 9 cut(s) 576, 603, 1328, 1677, 1977, 2161, 2420, 2932, 3060
BspQI GCTCTTC 4 cut(s) 123, 225, 2045, 2509
BspT107I GGYRCC 1 cut(s) 2864
BspTNI GGTCTC 1 cut(s) 2823
BsrBI CCGCTC 1 cut(s) 145
BsrDI GCAATG 3 cut(s) 928, 1635, 2012
BsrFI RCCGGY 1 cut(s) 2968
BsrGI TGTACA 1 cut(s) 3291
BsrI ACTGG 3 cut(s) 576, 962, 1243
BssAI RCCGGY 1 cut(s) 2968
BssECI CCNNGG 9 cut(s) 1176, 1928, 2099, 2398, 2805, 2833, 3159, 3299, 3475
BssNI GRCGYC 1 cut(s) 1608
BssT1I CCWWGG 3 cut(s) 2099, 2833, 3159
Bst2UI CCWGG 3 cut(s) 1178, 1434, 3242
Bst4CI ACNGT 8 cut(s) 542, 786, 1471, 1867, 2695, 2849, 2933, 3536
BstACI GRCGYC 1 cut(s) 1608
BstAUI TGTACA 1 cut(s) 3291
BstC8I GCNNGC 3 cut(s) 31, 1813, 3472
BstDSI CCRYGG 3 cut(s) 2099, 2398, 3475
BstENI CCTNNNNNAGG 1 cut(s) 153
BstFNI CGCG 2 cut(s) 1573, 2685
BstH2I RGCGCY 1 cut(s) 1840
BstHHI GCGC 4 cut(s) 192, 1839, 1902, 2685
BstMAI GTCTC 7 cut(s) 880, 931, 1615, 1820, 2363, 2701, 2823
BstNI CCWGG 3 cut(s) 1178, 1434, 3242
BstNSI RCATGY 3 cut(s) 1885, 2075, 2348
BstPAI GACNNNNGTC 1 cut(s) 1215
BstSCI CCNGG 4 cut(s) 430, 1176, 1432, 3240
BstSFI CTRYAG 6 cut(s) 777, 1227, 1251, 1421, 1470, 3562
BstUI CGCG 2 cut(s) 1573, 2685
BstV2I GAAGAC 1 cut(s) 543
Bsu15I ATCGAT 2 cut(s) 1849, 2524
BsuI GTATCC 2 cut(s) 595, 2983
BsuRI GGCC 8 cut(s) 148, 476, 666, 682, 711, 2265, 2397, 3474
BsuTUI ATCGAT 2 cut(s) 1849, 2524
BtgI CCRYGG 3 cut(s) 2099, 2398, 3475
BtrI CACGTC 1 cut(s) 3521
BtsI GCAGTG 2 cut(s) 40, 1162
BtsIMutI CAGTG 8 cut(s) 40, 295, 583, 868, 1162, 2733, 2781, 2854
Cac8I GCNNGC 3 cut(s) 31, 1813, 3472
CciI TCATGA 3 cut(s) 691, 2209, 2914
CfoI GCGC 4 cut(s) 192, 1839, 1902, 2685
Cfr10I RCCGGY 1 cut(s) 2968
Cfr13I GGNCC 8 cut(s) 474, 680, 709, 2966, 3022, 3153, 3163, 3238
ClaI ATCGAT 2 cut(s) 1849, 2524
Csp6I GTAC 6 cut(s) 379, 916, 2420, 2567, 3292, 3324
CspAI ACCGGT 1 cut(s) 2968
CviQI GTAC 6 cut(s) 379, 916, 2420, 2567, 3292, 3324
DraI TTTAAA 2 cut(s) 1488, 2127
DrdI GACNNNNNNGTC 1 cut(s) 2990
DseDI GACNNNNNNGTC 1 cut(s) 2990
EaeI YGGCCR 1 cut(s) 2395
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 3 cut(s) 2099, 2833, 3159
Eco24I GRGCYC 1 cut(s) 861
Eco31I GGTCTC 1 cut(s) 2823
Eco32I GATATC 1 cut(s) 3530
Eco47I GGWCC 5 cut(s) 2966, 3022, 3153, 3163, 3238
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 3 cut(s) 236, 727, 2159
Eco88I CYCGRG 1 cut(s) 3300
EcoICRI GAGCTC 1 cut(s) 859
EcoNI CCTNNNNNAGG 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 709
EcoRII CCWGG 3 cut(s) 1176, 1432, 3240
EcoRV GATATC 1 cut(s) 3530
EcoT14I CCWWGG 3 cut(s) 2099, 2833, 3159
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 3 cut(s) 2099, 2833, 3159
Esp3I CGTCTC 1 cut(s) 1615
FalI AAGNNNNNCTT 6 cut(s) 725, 757, 2024, 2056, 3396, 3428
FaqI GGGAC 2 cut(s) 1414, 1520
FauI CCCGC 3 cut(s) 231, 1204, 3542
FauNDI CATATG 3 cut(s) 904, 1058, 2310
FbaI TGATCA 3 cut(s) 1159, 1459, 2821
FblI GTMKAC 1 cut(s) 2060
FriOI GRGCYC 1 cut(s) 861
GlaI GCGC 4 cut(s) 191, 1838, 1901, 2684
GsuI CTGGAG 2 cut(s) 1260, 3243
HaeII RGCGCY 1 cut(s) 1840
HaeIII GGCC 8 cut(s) 148, 476, 666, 682, 711, 2265, 2397, 3474
HapII CCGG 5 cut(s) 431, 683, 1121, 2969, 3546
HhaI GCGC 4 cut(s) 192, 1839, 1902, 2685
Hin1I GRCGYC 1 cut(s) 1608
Hin6I GCGC 4 cut(s) 190, 1837, 1900, 2683
HinP1I GCGC 4 cut(s) 190, 1837, 1900, 2683
HincII GTYRAC 4 cut(s) 271, 1220, 1467, 2236
HindII GTYRAC 4 cut(s) 271, 1220, 1467, 2236
HindIII AAGCTT 2 cut(s) 320, 1511
HpaII CCGG 5 cut(s) 431, 683, 1121, 2969, 3546
HphI GGTGA 5 cut(s) 1364, 2117, 2179, 2915, 3256
HpyCH4III ACNGT 8 cut(s) 542, 786, 1471, 1867, 2695, 2849, 2933, 3536
HpyCH4IV ACGT 3 cut(s) 1608, 3088, 3520
HpySE526I ACGT 3 cut(s) 1608, 3088, 3520
Hsp92I GRCGYC 1 cut(s) 1608
HspAI GCGC 4 cut(s) 190, 1837, 1900, 2683
Ksp22I TGATCA 3 cut(s) 1159, 1459, 2821
LguI GCTCTTC 4 cut(s) 123, 225, 2045, 2509
LmnI GCTCC 8 cut(s) 4, 820, 1552, 1626, 2680, 3082, 3409, 3515
LweI GCATC 6 cut(s) 304, 1000, 1402, 2026, 2277, 2523
MaeII ACGT 3 cut(s) 1608, 3088, 3520
MaeIII GTNAC 7 cut(s) 298, 980, 994, 1993, 2483, 2843, 3266
MbiI CCGCTC 1 cut(s) 145
MhlI GDGCHC 4 cut(s) 9, 861, 2079, 3520
MlsI TGGCCA 1 cut(s) 2397
MluNI TGGCCA 1 cut(s) 2397
MlyI GAGTC 6 cut(s) 616, 1154, 2365, 2377, 2623, 3227
MmeI TCCRAC 7 cut(s) 505, 1614, 1866, 2754, 2787, 3163, 3189
Mox20I TGGCCA 1 cut(s) 2397
MroXI GAANNNNTTC 2 cut(s) 740, 1036
MscI TGGCCA 1 cut(s) 2397
MslI CAYNNNNRTG 3 cut(s) 828, 2453, 2919
Msp20I TGGCCA 1 cut(s) 2397
MspA1I CMGCKG 5 cut(s) 200, 238, 1022, 1745, 2192
MspI CCGG 5 cut(s) 431, 683, 1121, 2969, 3546
MspR9I CCNGG 4 cut(s) 432, 1178, 1434, 3242
Mva1269I GAATGC 1 cut(s) 1097
MvaI CCWGG 3 cut(s) 1178, 1434, 3242
MvnI CGCG 2 cut(s) 1573, 2685
NciI CCSGG 1 cut(s) 432
NcoI CCATGG 1 cut(s) 2099
NdeI CATATG 3 cut(s) 904, 1058, 2310
NlaIV GGNNCC 5 cut(s) 2803, 2866, 3024, 3154, 3428
NmeAIII GCCGAG 2 cut(s) 55, 1909
NmuCI GTSAC 4 cut(s) 980, 1993, 2483, 2843
NspI RCATGY 3 cut(s) 1885, 2075, 2348
PacI TTAATTAA 1 cut(s) 1659
PagI TCATGA 3 cut(s) 691, 2209, 2914
PciI ACATGT 1 cut(s) 1881
PciSI GCTCTTC 4 cut(s) 123, 225, 2045, 2509
PctI GAATGC 1 cut(s) 1097
PdmI GAANNNNTTC 2 cut(s) 740, 1036
PfeI GAWTC 8 cut(s) 176, 1037, 1205, 2316, 2351, 2526, 2852, 2911
PflMI CCANNNNNTGG 2 cut(s) 677, 977
PfoI TCCNGGA 1 cut(s) 430
PinAI ACCGGT 1 cut(s) 2968
PleI GAGTC 6 cut(s) 616, 1153, 2365, 2377, 2623, 3226
PpsI GAGTC 6 cut(s) 616, 1153, 2365, 2377, 2623, 3226
PscI ACATGT 1 cut(s) 1881
PshAI GACNNNNGTC 1 cut(s) 1215
PshBI ATTAAT 3 cut(s) 1659, 1895, 2871
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 3 cut(s) 1176, 1432, 3240
PspGI CCWGG 3 cut(s) 1176, 1432, 3240
PspN4I GGNNCC 5 cut(s) 2803, 2866, 3024, 3154, 3428
PspPI GGNCC 8 cut(s) 474, 680, 709, 2966, 3022, 3153, 3163, 3238
PstI CTGCAG 3 cut(s) 781, 1255, 1425
PvuII CAGCTG 1 cut(s) 1745
RsaI GTAC 6 cut(s) 380, 917, 2421, 2568, 3293, 3325
RsaNI GTAC 6 cut(s) 379, 916, 2420, 2567, 3292, 3324
RseI CAYNNNNRTG 3 cut(s) 828, 2453, 2919
SacI GAGCTC 1 cut(s) 861
SapI GCTCTTC 4 cut(s) 123, 225, 2045, 2509
Sau96I GGNCC 8 cut(s) 474, 680, 709, 2966, 3022, 3153, 3163, 3238
SchI GAGTC 6 cut(s) 616, 1154, 2365, 2377, 2623, 3227
ScrFI CCNGG 4 cut(s) 432, 1178, 1434, 3242
SduI GDGCHC 4 cut(s) 9, 861, 2079, 3520
SfaNI GCATC 6 cut(s) 304, 1000, 1402, 2026, 2277, 2523
SfcI CTRYAG 6 cut(s) 777, 1227, 1251, 1421, 1470, 3562
SinI GGWCC 5 cut(s) 2966, 3022, 3153, 3163, 3238
SmiI ATTTAAAT 1 cut(s) 1488
SmiMI CAYNNNNRTG 3 cut(s) 828, 2453, 2919
SmlI CTYRAG 6 cut(s) 80, 259, 469, 1823, 2675, 3027
SmoI CTYRAG 6 cut(s) 80, 259, 469, 1823, 2675, 3027
SpeI ACTAGT 1 cut(s) 998
SspI AATATT 1 cut(s) 1893
SstI GAGCTC 1 cut(s) 861
StyD4I CCNGG 4 cut(s) 430, 1176, 1432, 3240
StyI CCWWGG 3 cut(s) 2099, 2833, 3159
SwaI ATTTAAAT 1 cut(s) 1488
TaaI ACNGT 8 cut(s) 542, 786, 1471, 1867, 2695, 2849, 2933, 3536
TaiI ACGT 3 cut(s) 1611, 3091, 3523
TaqI TCGA 5 cut(s) 1849, 2497, 2524, 2632, 2875
TatI WGTACW 3 cut(s) 2419, 2566, 3291
TauI GCSGC 2 cut(s) 148, 2205
TfiI GAWTC 8 cut(s) 176, 1037, 1205, 2316, 2351, 2526, 2852, 2911
TscAI CASTG 8 cut(s) 40, 295, 583, 868, 1162, 2740, 2781, 2854
TseFI GTSAC 4 cut(s) 980, 1993, 2483, 2843
Tsp45I GTSAC 4 cut(s) 980, 1993, 2483, 2843
TspGWI ACGGA 2 cut(s) 1994, 2314
TspRI CASTG 8 cut(s) 40, 295, 583, 868, 1162, 2740, 2781, 2854
Van91I CCANNNNNTGG 2 cut(s) 677, 977
VpaK11BI GGWCC 5 cut(s) 2966, 3022, 3153, 3163, 3238
VspI ATTAAT 3 cut(s) 1659, 1895, 2871
XagI CCTNNNNNAGG 1 cut(s) 153
XapI RAATTY 9 cut(s) 281, 616, 687, 737, 1405, 1489, 1918, 2216, 2585
XbaI TCTAGA 1 cut(s) 2245
XceI RCATGY 3 cut(s) 1885, 2075, 2348
XmiI GTMKAC 1 cut(s) 2060
XmnI GAANNNNTTC 2 cut(s) 740, 1036
ZraI GACGTC 1 cut(s) 1609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.