FvH4_4g12850

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007064
Physical Location & Seq
Forward (+)
16508027 .. 16516435
8409 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12850.t1

Sequence Viewer

Length: 2313 bp
ATGTCGGAGATGGCGAACACCGACCCCGAGGGCATCGACGGCGTCCGCATGACGTGGAACGTGTGGCCCCGCACGAAGGTCGAGGCCAGCAAGTGCGTGATCCCACTCGCCGCCTGCATCGCCCCGATCCGGTTCCACACCGACATCCCCACGCTCCCCTACGCGCCTCTCCGCTGCAAGACCTGCTCCGCCGTCCTCAACCCTTTCTCCCGCGTCGACTTCACCGCCAAGATCTGGATCTGCCCATTCTGCTACCAACGCAATCACTTCCCTCCTCACTACTCTCTCATCTCCGAGACCAATCTCCCCGGCGAGCTCTACCCCCAGTACACCACCGTCCAGTACTCCCTCCCCGCCGATTCCACCTCCCCGCCGGCACCGGTGTTCGTCTTCGTGCTCGACACGTGCATGATCGAGGAGGAGATGGGGTATGTCAAATCGGCGCTGAAGCAGGCGATCGGGCTGCTGCCGGAGAATGCTCTGGTGGGGTTCATCTCCTTCGGGACTCAGGTGCAGGTCCATGAGCTTGGGTTCGCCGAATTGAGCAAGGTCTATGTGTTTCGGGGGACTAAGGAGATTACCAAAGATCAGGTGCTGGAGCAATTGGGACTTGGGAGCTCCGGCCGGAGGCCTGGTCCTATTGGTGGAGGGTATAATCAGCCTAAGGGGATTCAGAATGGGTTTCCTAATTCGGGGGTCACCAGATTTCTCTTGCCCGCTTCCGAGTGCGAATTTACTCTCAATTCGTTGTTGGATGAATTGCAAAGCGACCAGTGGCCTGTGCCTCCTGGGAATAGGTCGTCTAGGTGCACCGGAGTGGCGGTGAGTGTTGCAGCCGGTTTGCTAGGGGCTTGTGTGGCAGGAACTGGGGCAAGGATTGTAGCCTTGGTTGGTGGTCCTTGCACTGAAGGGCCAGGCGCGATCGTTTCAAAAGATCTATCAGAACCTGTACGTTCCCATAAAGATCTGGATAAGGATGCGGCTCCGTACTTCAAGAAAGCAGTCAAATTTTATGATAGTCTTGCGAAGCAGCTGGTTAGCCAGGGTCATGTCTTAGACCTATTTGCATCTGCTCTTGATCAGGTTGGTGTTGCTGAAATGAAAGTTGCAATTGAGAAAACAGGTGGACTTGTTGTTCTGGCAGAAAGTTTTGGTCATTCTGTATTCAAAGACTCTTTCAGGCGTTTCTTTGAACAAGGGGAGCAGTCTCTTGGCCTTTGTTTTAATGGCACCCTTGAGATCAACTGTTCAAAGGACATCAAAATTCAGGGGGTCATCGGACCGTGCACATCATTGGAGAAGAAAGGACCTGCTGTTGCCGATACTTCTATAGGGGAAGGGAATACAACAGCATGGAAAATGTGTAGCCTTGACATGAGTACTTGCTTGACAGTTTTCTTTGATCTATCATCAAGTGATCAGTCAAATACACCAGGAACAGTAAACCCACAATTGTATTTGCAGTTCCTTACCAGCTTTCAAAATCCTCAAGGTCAAATGATGCTTCGAGTAACAACAATTACTAGACGATGGATAGATAGTTCTGTTAGCTCAGAGGAACTGGTTCAAGGATTTGACCAAGAGACTGCTGCTGTAGTAATGGCCAGAAAAACTTCTTTGAAAATGGAGACAGAGGAGTCATTTGATGCTACCCGATGGCTGGATCGGGGACTTATCCGTCTCTGTTCCAAATTTGGTGACTATCGGAAAGATGATCCAGCATCCTTTACTTTGAATCCATGTTTTTCTTTATTCCCTCAATTTATGTTCAATCTACGCCGCTCGCAATTTGTACAGGTTTTCAACAACAGTCCAGACGAAACTGCCTATTTTCGCATGCTGTTGAATCGGGAAAATATCACCAATGCTGCTGTCATGATACAACCATCATTAATATCATACTCATTTAATTCTCTACCTCAGCCAGCCTTGTTGGATGTTGCTTCCATTGCAGCTGACCGTATTCTGTTGTTGGATTCATATTTTAGTATAGTCATTTTCCATGGAATGACAATAGCACAGTGGCGTAACATGGGATACCAGAATCAGCAAGAACACGTGGCATTTGCACAATTATTGCAAGCCCCTCATGATGATACACAGATGATCATCCGTGAACGTTTCCCTGTCCCTAGGTTGGTCGTCTGCGACCAGCATGGTTCCCAGGCGAGGTTCTTATTAGCAAAGTTGAACCCTTCAGCAACGTACAACAATGCACATGAGATGGCTGCTGGATCAGATGTGATCTTCACAGATGATGTAAGTCTTCAGGTCTTTTTCGAGCATCTTCAGAGGCTGGCAGTGCAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

771

Amino Acids

84.94

Weight (kDa)

5.65

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 56 - 94 8.7e-17 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 124 - 396 6e-67 Sec23/Sec24 trunk domain
Sec23_BS PF08033 408 - 511 4.4e-23 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 526 - 623 9.8e-26 Sec23/Sec24 helical domain
Gelsolin PF00626 638 - 726 1.1e-14 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1636, 1677
Acc36I ACCTGC 3 cut(s) 191, 505, 1318
AccB1I GGYRCC 2 cut(s) 376, 1229
AccB7I CCANNNNNTGG 1 cut(s) 234
AccBSI CCGCTC 1 cut(s) 1782
AccI GTMKAC 1 cut(s) 216
AccII CGCG 3 cut(s) 164, 213, 920
AclI AACGTT 1 cut(s) 2119
AclWI GGATC 6 cut(s) 94, 121, 245, 1671, 1709, 2242
AcoI YGGCCR 2 cut(s) 622, 1602
AcsI RAATTY 4 cut(s) 731, 1007, 1263, 1691
AcuI CTGAAG 5 cut(s) 467, 927, 2181, 2252, 2273
AcvI CACGTG 2 cut(s) 405, 2059
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 7 cut(s) 329, 344, 951, 989, 1381, 1794, 2207
AfiI CCNNNNNNNGG 9 cut(s) 76, 129, 234, 627, 644, 692, 1660, 2137, 2169
AflIII ACRYGT 3 cut(s) 60, 402, 2056
AgeI ACCGGT 1 cut(s) 379
AjiI CACGTC 1 cut(s) 54
AjnI CCWGG 6 cut(s) 631, 787, 913, 1041, 1432, 2163
AleI CACNNNNGTG 1 cut(s) 815
AloI GAACNNNNNNTCC 4 cut(s) 1119, 1151, 1525, 1557
AluBI AGCT 7 cut(s) 316, 526, 618, 1033, 1476, 1551, 1955
AluI AGCT 7 cut(s) 316, 526, 618, 1033, 1476, 1551, 1955
Alw21I GWGCWC 5 cut(s) 318, 399, 620, 812, 1289
Alw26I GTCTC 5 cut(s) 290, 1212, 1577, 1622, 1685
Alw44I GTGCAC 2 cut(s) 808, 1285
AlwI GGATC 6 cut(s) 94, 121, 245, 1671, 1709, 2242
AlwNI CAGNNNCTG 4 cut(s) 595, 866, 947, 2296
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 8 cut(s) 65, 84, 622, 629, 776, 911, 1213, 1602
ApaLI GTGCAC 2 cut(s) 808, 1285
ApeKI GCWGC 9 cut(s) 174, 463, 466, 833, 1030, 1589, 1868, 1952, 2228
ApoI RAATTY 4 cut(s) 731, 1007, 1263, 1691
AseI ATTAAT 1 cut(s) 1892
AsiGI ACCGGT 1 cut(s) 379
Asp700I GAANNNNTTC 2 cut(s) 1563, 1612
AspA2I CCTAGG 1 cut(s) 2132
AspLEI GCGC 3 cut(s) 166, 445, 920
AspS9I GGNCC 7 cut(s) 66, 517, 635, 896, 911, 1280, 1307
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 5 cut(s) 214, 691, 835, 1709, 1852
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 5 cut(s) 517, 635, 896, 1280, 1307
AvrII CCTAGG 1 cut(s) 2132
AxyI CCTNAGG 1 cut(s) 663
BaeGI GKGCMC 2 cut(s) 812, 1289
BalI TGGCCA 1 cut(s) 1604
BanI GGYRCC 2 cut(s) 376, 1229
BanII GRGCYC 2 cut(s) 318, 620
BbrPI CACGTG 2 cut(s) 405, 2059
BbsI GAAGAC 2 cut(s) 382, 2258
Bbv12I GWGCWC 5 cut(s) 318, 399, 620, 812, 1289
BbvCI CCTCAGC 1 cut(s) 1920
BbvI GCAGC 9 cut(s) 161, 450, 453, 845, 1042, 1576, 1855, 1964, 2215
BccI CCATC 6 cut(s) 4, 418, 1524, 1650, 1894, 2218
BceAI ACGGC 2 cut(s) 55, 176
BcgI CGANNNNNNTGC 4 cut(s) 61, 95, 445, 479
BciT130I CCWGG 6 cut(s) 633, 789, 915, 1043, 1434, 2165
BciVI GTATCC 1 cut(s) 2030
BclI TGATCA 3 cut(s) 1078, 1417, 2106
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 5 cut(s) 290, 1212, 1577, 1622, 1685
BfaI CTAG 4 cut(s) 804, 845, 1524, 2133
BfmI CTRYAG 2 cut(s) 1329, 1593
BfoI RGCGCY 1 cut(s) 446
BfuAI ACCTGC 3 cut(s) 191, 505, 1318
BfuI GTATCC 1 cut(s) 2030
BglII AGATCT 3 cut(s) 231, 934, 964
BlnI CCTAGG 1 cut(s) 2132
BmcAI AGTACT 2 cut(s) 344, 1381
Bme1390I CCNGG 7 cut(s) 309, 633, 789, 915, 1043, 1434, 2165
Bme18I GGWCC 5 cut(s) 517, 635, 896, 1280, 1307
BmeT110I CYCGRG 1 cut(s) 26
BmgBI CACGTC 1 cut(s) 54
BmgT120I GGNCC 7 cut(s) 66, 517, 635, 896, 911, 1280, 1307
BmiI GGNNCC 6 cut(s) 68, 134, 378, 984, 1231, 2161
BmrFI CCNGG 7 cut(s) 309, 633, 789, 915, 1043, 1434, 2165
BmrI ACTGGG 2 cut(s) 319, 876
BmsI GCATC 8 cut(s) 42, 126, 967, 1076, 1491, 1636, 1730, 2293
BmuI ACTGGG 2 cut(s) 319, 876
BpiI GAAGAC 2 cut(s) 382, 2258
BpmI CTGGAG 1 cut(s) 617
Bpu10I CCTNAGC 1 cut(s) 1920
BpuEI CTTGAG 2 cut(s) 1256, 1473
BpuMI CCSGG 1 cut(s) 309
BsaAI YACGTR 2 cut(s) 405, 2059
BsaBI GATNNNNATC 2 cut(s) 236, 2108
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 8 cut(s) 27, 307, 788, 885, 1042, 2002, 2132, 2163
BsaWI WCCGGW 3 cut(s) 129, 379, 812
BsaXI ACNNNNNCTCC 8 cut(s) 191, 221, 413, 443, 619, 649, 807, 837
Bsc4I CCNNNNNNNGG 9 cut(s) 76, 129, 234, 627, 644, 692, 1660, 2137, 2169
Bse118I RCCGGY 3 cut(s) 373, 379, 836
Bse1I ACTGG 5 cut(s) 325, 340, 772, 871, 1566
Bse21I CCTNAGG 1 cut(s) 663
Bse3DI GCAATG 1 cut(s) 1947
Bse8I GATNNNNATC 2 cut(s) 236, 2108
BseBI CCWGG 6 cut(s) 633, 789, 915, 1043, 1434, 2165
BseDI CCNNGG 8 cut(s) 27, 307, 788, 885, 1042, 2002, 2132, 2163
BseGI GGATG 6 cut(s) 144, 760, 982, 1721, 1942, 2109
BseJI GATNNNNATC 2 cut(s) 236, 2108
BseLI CCNNNNNNNGG 9 cut(s) 76, 129, 234, 627, 644, 692, 1660, 2137, 2169
BseMI GCAATG 1 cut(s) 1947
BseMII CTCAG 3 cut(s) 521, 1566, 1934
BseNI ACTGG 5 cut(s) 325, 340, 772, 871, 1566
BseRI GAGGAG 4 cut(s) 264, 431, 434, 1649
BseSI GKGCMC 2 cut(s) 812, 1289
BseX3I CGGCCG 1 cut(s) 622
BseXI GCAGC 9 cut(s) 161, 450, 453, 845, 1042, 1576, 1855, 1964, 2215
BsgI GTGCAG 1 cut(s) 533
Bsh1236I CGCG 3 cut(s) 164, 213, 920
Bsh1285I CGRYCG 3 cut(s) 459, 625, 924
BshFI GGCC 8 cut(s) 67, 86, 624, 631, 778, 913, 1215, 1604
BshNI GGYRCC 2 cut(s) 376, 1229
BshTI ACCGGT 1 cut(s) 379
BsiEI CGRYCG 3 cut(s) 459, 625, 924
BsiHKAI GWGCWC 5 cut(s) 318, 399, 620, 812, 1289
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 9 cut(s) 130, 309, 374, 380, 470, 621, 625, 813, 837
BslFI GGGAC 5 cut(s) 517, 580, 621, 1683, 2114
BslI CCNNNNNNNGG 9 cut(s) 76, 129, 234, 627, 644, 692, 1660, 2137, 2169
BsmAI GTCTC 5 cut(s) 290, 1212, 1577, 1622, 1685
BsmBI CGTCTC 1 cut(s) 1685
BsmFI GGGAC 5 cut(s) 517, 580, 621, 1683, 2114
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 8 cut(s) 67, 86, 624, 631, 778, 913, 1215, 1604
Bso31I GGTCTC 1 cut(s) 290
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 5 cut(s) 318, 399, 620, 812, 1289
Bsp1407I TGTACA 1 cut(s) 1792
Bsp19I CCATGG 1 cut(s) 2002
BspANI GGCC 8 cut(s) 67, 86, 624, 631, 778, 913, 1215, 1604
BspCNI CTCAG 3 cut(s) 520, 1565, 1933
BspFNI CGCG 3 cut(s) 164, 213, 920
BspHI TCATGA 2 cut(s) 1875, 2089
BspLI GGNNCC 6 cut(s) 68, 134, 378, 984, 1231, 2161
BspMI ACCTGC 3 cut(s) 191, 505, 1318
BspPI GGATC 6 cut(s) 94, 121, 245, 1671, 1709, 2242
BspT107I GGYRCC 2 cut(s) 376, 1229
BspTNI GGTCTC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 1782
BsrDI GCAATG 1 cut(s) 1947
BsrFI RCCGGY 3 cut(s) 373, 379, 836
BsrGI TGTACA 1 cut(s) 1792
BsrI ACTGG 5 cut(s) 325, 340, 772, 871, 1566
BssAI RCCGGY 3 cut(s) 373, 379, 836
BssECI CCNNGG 8 cut(s) 27, 307, 788, 885, 1042, 2002, 2132, 2163
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 3 cut(s) 885, 2002, 2132
Bst2UI CCWGG 6 cut(s) 633, 789, 915, 1043, 1434, 2165
Bst4CI ACNGT 8 cut(s) 337, 1247, 1284, 1393, 1441, 1811, 1961, 2022
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 183
BstAUI TGTACA 1 cut(s) 1792
BstBAI YACGTR 2 cut(s) 405, 2059
BstDEI CTNAG 7 cut(s) 507, 570, 663, 1054, 1552, 1920, 2310
BstDSI CCRYGG 1 cut(s) 2002
BstEII GGTNACC 1 cut(s) 697
BstF5I GGATG 6 cut(s) 144, 760, 982, 1721, 1942, 2109
BstFNI CGCG 3 cut(s) 164, 213, 920
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 3 cut(s) 166, 445, 920
BstMAI GTCTC 5 cut(s) 290, 1212, 1577, 1622, 1685
BstMCI CGRYCG 3 cut(s) 459, 625, 924
BstMWI GCNNNNNNNGC 8 cut(s) 39, 119, 183, 249, 258, 857, 1949, 2302
BstNI CCWGG 6 cut(s) 633, 789, 915, 1043, 1434, 2165
BstNSI RCATGY 1 cut(s) 1840
BstPI GGTNACC 1 cut(s) 697
BstSCI CCNGG 7 cut(s) 307, 631, 787, 913, 1041, 1432, 2163
BstSFI CTRYAG 2 cut(s) 1329, 1593
BstSLI GKGCMC 2 cut(s) 812, 1289
BstUI CGCG 3 cut(s) 164, 213, 920
BstV1I GCAGC 9 cut(s) 161, 450, 453, 845, 1042, 1576, 1855, 1964, 2215
BstV2I GAAGAC 2 cut(s) 382, 2258
BstX2I RGATCY 4 cut(s) 231, 237, 934, 964
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 4 cut(s) 231, 237, 934, 964
BstZI CGGCCG 1 cut(s) 622
Bsu36I CCTNAGG 1 cut(s) 663
BsuI GTATCC 1 cut(s) 2030
BsuRI GGCC 8 cut(s) 67, 86, 624, 631, 778, 913, 1215, 1604
BtgI CCRYGG 1 cut(s) 2002
BtgZI GCGATG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 6 cut(s) 144, 760, 982, 1721, 1942, 2109
BtsI GCAGTG 1 cut(s) 2307
BtsIMutI CAGTG 4 cut(s) 779, 903, 2027, 2307
BveI ACCTGC 3 cut(s) 191, 505, 1318
CaiI CAGNNNCTG 4 cut(s) 595, 866, 947, 2296
CciI TCATGA 2 cut(s) 1875, 2089
CfoI GCGC 3 cut(s) 166, 445, 920
Cfr10I RCCGGY 3 cut(s) 373, 379, 836
Cfr13I GGNCC 7 cut(s) 66, 517, 635, 896, 911, 1280, 1307
CpoI CGGWCCG 1 cut(s) 1280
CseI GACGC 2 cut(s) 31, 202
Csp6I GTAC 7 cut(s) 328, 343, 950, 988, 1380, 1793, 2206
CspAI ACCGGT 1 cut(s) 379
CspI CGGWCCG 1 cut(s) 1280
CviQI GTAC 7 cut(s) 328, 343, 950, 988, 1380, 1793, 2206
DdeI CTNAG 7 cut(s) 507, 570, 663, 1054, 1552, 1920, 2310
DrdI GACNNNNNNGTC 2 cut(s) 1636, 1677
DseDI GACNNNNNNGTC 2 cut(s) 1636, 1677
EaeI YGGCCR 2 cut(s) 622, 1602
EagI CGGCCG 1 cut(s) 622
EciI GGCGGA 1 cut(s) 178
Ecl136II GAGCTC 2 cut(s) 316, 618
EclXI CGGCCG 1 cut(s) 622
Eco130I CCWWGG 3 cut(s) 885, 2002, 2132
Eco147I AGGCCT 1 cut(s) 631
Eco24I GRGCYC 2 cut(s) 318, 620
Eco31I GGTCTC 1 cut(s) 290
Eco47I GGWCC 5 cut(s) 517, 635, 896, 1280, 1307
Eco52I CGGCCG 1 cut(s) 622
Eco53kI GAGCTC 2 cut(s) 316, 618
Eco57I CTGAAG 5 cut(s) 467, 927, 2181, 2252, 2273
Eco72I CACGTG 2 cut(s) 405, 2059
Eco81I CCTNAGG 1 cut(s) 663
Eco88I CYCGRG 1 cut(s) 26
Eco91I GGTNACC 1 cut(s) 697
EcoICRI GAGCTC 2 cut(s) 316, 618
EcoO109I RGGNCCY 1 cut(s) 1307
EcoO65I GGTNACC 1 cut(s) 697
EcoRII CCWGG 6 cut(s) 631, 787, 913, 1041, 1432, 2163
EcoT14I CCWWGG 3 cut(s) 885, 2002, 2132
EcoT38I GRGCYC 2 cut(s) 318, 620
ErhI CCWWGG 3 cut(s) 885, 2002, 2132
Esp3I CGTCTC 1 cut(s) 1685
FaqI GGGAC 5 cut(s) 517, 580, 621, 1683, 2114
FauI CCCGC 5 cut(s) 77, 218, 361, 378, 724
FbaI TGATCA 3 cut(s) 1078, 1417, 2106
FblI GTMKAC 1 cut(s) 216
FokI GGATG 6 cut(s) 131, 767, 989, 1708, 1949, 2096
FriOI GRGCYC 2 cut(s) 318, 620
FspBI CTAG 4 cut(s) 804, 845, 1524, 2133
GlaI GCGC 3 cut(s) 165, 444, 919
GsuI CTGGAG 1 cut(s) 617
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 8 cut(s) 67, 86, 624, 631, 778, 913, 1215, 1604
HapII CCGG 9 cut(s) 130, 309, 374, 380, 470, 621, 625, 813, 837
HgaI GACGC 2 cut(s) 31, 202
HhaI GCGC 3 cut(s) 166, 445, 920
Hin1I GRCGYC 1 cut(s) 42
Hin6I GCGC 3 cut(s) 164, 443, 918
HinP1I GCGC 3 cut(s) 164, 443, 918
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 9 cut(s) 359, 505, 670, 1172, 1637, 1735, 1846, 1976, 2044
HpaII CCGG 9 cut(s) 130, 309, 374, 380, 470, 621, 625, 813, 837
HphI GGTGA 5 cut(s) 214, 691, 835, 1709, 1852
Hpy166II GTNNAC 7 cut(s) 217, 330, 810, 1127, 1287, 1444, 2117
Hpy188III TCNNGA 9 cut(s) 235, 502, 968, 994, 1076, 1814, 1850, 1876, 2090
Hpy8I GTNNAC 7 cut(s) 217, 330, 810, 1127, 1287, 1444, 2117
Hpy99I CGWCG 2 cut(s) 41, 218
HpyAV CCTTC 5 cut(s) 70, 508, 902, 1331, 2205
HpyCH4III ACNGT 8 cut(s) 337, 1247, 1284, 1393, 1441, 1811, 1961, 2022
HpyCH4IV ACGT 7 cut(s) 53, 60, 404, 952, 2058, 2119, 2204
HpyF10VI GCNNNNNNNGC 8 cut(s) 39, 119, 183, 249, 258, 857, 1949, 2302
HpyF3I CTNAG 7 cut(s) 507, 570, 663, 1054, 1552, 1920, 2310
HpySE526I ACGT 7 cut(s) 53, 60, 404, 952, 2058, 2119, 2204
Hsp92I GRCGYC 1 cut(s) 42
HspAI GCGC 3 cut(s) 164, 443, 918
KroI GCCGGC 1 cut(s) 373
KroNI GCCGGC 1 cut(s) 375
Ksp22I TGATCA 3 cut(s) 1078, 1417, 2106
LmnI GCTCC 7 cut(s) 159, 191, 598, 615, 623, 988, 1201
Lsp1109I GCAGC 9 cut(s) 161, 450, 453, 845, 1042, 1576, 1855, 1964, 2215
LweI GCATC 8 cut(s) 42, 126, 967, 1076, 1491, 1636, 1730, 2293
MaeI CTAG 4 cut(s) 804, 845, 1524, 2133
MaeII ACGT 7 cut(s) 53, 60, 404, 952, 2058, 2119, 2204
MaeIII GTNAC 4 cut(s) 697, 1510, 1697, 2027
MbiI CCGCTC 1 cut(s) 1782
MboII GAAGA 5 cut(s) 382, 1312, 2239, 2258, 2279
MfeI CAATTG 3 cut(s) 602, 1110, 1451
MflI RGATCY 4 cut(s) 231, 237, 934, 964
MhlI GDGCHC 5 cut(s) 318, 399, 620, 812, 1289
MlsI TGGCCA 1 cut(s) 1604
MluNI TGGCCA 1 cut(s) 1604
MlyI GAGTC 3 cut(s) 499, 1166, 1646
MmeI TCCRAC 3 cut(s) 732, 1914, 1953
Mox20I TGGCCA 1 cut(s) 1604
MroNI GCCGGC 1 cut(s) 373
MroXI GAANNNNTTC 2 cut(s) 1563, 1612
MscI TGGCCA 1 cut(s) 1604
MseI TTAA 3 cut(s) 1224, 1892, 1908
MslI CAYNNNNRTG 2 cut(s) 407, 815
Msp20I TGGCCA 1 cut(s) 1604
MspA1I CMGCKG 3 cut(s) 174, 1033, 1955
MspI CCGG 9 cut(s) 130, 309, 374, 380, 470, 621, 625, 813, 837
MspR9I CCNGG 7 cut(s) 309, 633, 789, 915, 1043, 1434, 2165
MunI CAATTG 3 cut(s) 602, 1110, 1451
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 6 cut(s) 633, 789, 915, 1043, 1434, 2165
MvnI CGCG 3 cut(s) 164, 213, 920
MwoI GCNNNNNNNGC 8 cut(s) 39, 119, 183, 249, 258, 857, 1949, 2302
NaeI GCCGGC 1 cut(s) 375
NciI CCSGG 1 cut(s) 309
NcoI CCATGG 1 cut(s) 2002
NgoMIV GCCGGC 1 cut(s) 373
NlaIV GGNNCC 6 cut(s) 68, 134, 378, 984, 1231, 2161
NmuCI GTSAC 2 cut(s) 697, 1697
NspI RCATGY 1 cut(s) 1840
OliI CACNNNNGTG 1 cut(s) 815
PaeI GCATGC 1 cut(s) 1840
PagI TCATGA 2 cut(s) 1875, 2089
PceI AGGCCT 1 cut(s) 631
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 1 cut(s) 481
PdiI GCCGGC 1 cut(s) 375
PdmI GAANNNNTTC 2 cut(s) 1563, 1612
PfeI GAWTC 6 cut(s) 359, 670, 1735, 1846, 1976, 2044
PflFI GACNNNGTC 1 cut(s) 41
PflMI CCANNNNNTGG 1 cut(s) 234
PinAI ACCGGT 1 cut(s) 379
Ple19I CGATCG 2 cut(s) 459, 924
PleI GAGTC 3 cut(s) 499, 1166, 1645
PmaCI CACGTG 2 cut(s) 405, 2059
PmlI CACGTG 2 cut(s) 405, 2059
PpsI GAGTC 3 cut(s) 499, 1166, 1645
Ppu21I YACGTR 2 cut(s) 405, 2059
PpuMI RGGWCCY 1 cut(s) 1307
PshBI ATTAAT 1 cut(s) 1892
Psp124BI GAGCTC 2 cut(s) 318, 620
Psp1406I AACGTT 1 cut(s) 2119
Psp5II RGGWCCY 1 cut(s) 1307
Psp6I CCWGG 6 cut(s) 631, 787, 913, 1041, 1432, 2163
PspCI CACGTG 2 cut(s) 405, 2059
PspEI GGTNACC 1 cut(s) 697
PspGI CCWGG 6 cut(s) 631, 787, 913, 1041, 1432, 2163
PspN4I GGNNCC 6 cut(s) 68, 134, 378, 984, 1231, 2161
PspPI GGNCC 7 cut(s) 66, 517, 635, 896, 911, 1280, 1307
PspPPI RGGWCCY 1 cut(s) 1307
PsrI GAACNNNNNNTAC 2 cut(s) 1448, 1480
PstNI CAGNNNCTG 4 cut(s) 595, 866, 947, 2296
PsuI RGATCY 4 cut(s) 231, 237, 934, 964
PsyI GACNNNGTC 1 cut(s) 41
PvuI CGATCG 2 cut(s) 459, 924
PvuII CAGCTG 2 cut(s) 1033, 1955
RsaI GTAC 7 cut(s) 329, 344, 951, 989, 1381, 1794, 2207
RsaNI GTAC 7 cut(s) 328, 343, 950, 988, 1380, 1793, 2206
RseI CAYNNNNRTG 2 cut(s) 407, 815
Rsr2I CGGWCCG 1 cut(s) 1280
RsrII CGGWCCG 1 cut(s) 1280
SacI GAGCTC 2 cut(s) 318, 620
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 3 cut(s) 1224, 1892, 1908
Sau96I GGNCC 7 cut(s) 66, 517, 635, 896, 911, 1280, 1307
ScaI AGTACT 2 cut(s) 344, 1381
SchI GAGTC 3 cut(s) 499, 1166, 1646
ScrFI CCNGG 7 cut(s) 309, 633, 789, 915, 1043, 1434, 2165
SduI GDGCHC 5 cut(s) 318, 399, 620, 812, 1289
SfaNI GCATC 8 cut(s) 42, 126, 967, 1076, 1491, 1636, 1730, 2293
SfcI CTRYAG 2 cut(s) 1329, 1593
SgrAI CRCCGGYG 1 cut(s) 379
SinI GGWCC 5 cut(s) 517, 635, 896, 1280, 1307
SmiMI CAYNNNNRTG 2 cut(s) 407, 815
SmlI CTYRAG 2 cut(s) 1235, 1488
SmoI CTYRAG 2 cut(s) 1235, 1488
SphI GCATGC 1 cut(s) 1840
SseBI AGGCCT 1 cut(s) 631
SspMI CTAG 4 cut(s) 804, 845, 1524, 2133
SstI GAGCTC 2 cut(s) 318, 620
StuI AGGCCT 1 cut(s) 631
StyD4I CCNGG 7 cut(s) 307, 631, 787, 913, 1041, 1432, 2163
StyI CCWWGG 3 cut(s) 885, 2002, 2132
TaaI ACNGT 8 cut(s) 337, 1247, 1284, 1393, 1441, 1811, 1961, 2022
TaiI ACGT 7 cut(s) 56, 63, 407, 955, 2061, 2122, 2207
TaqI TCGA 7 cut(s) 36, 81, 216, 399, 414, 1507, 2280
TatI WGTACW 4 cut(s) 327, 342, 1379, 1792
TauI GCSGC 3 cut(s) 113, 983, 1782
TfiI GAWTC 6 cut(s) 359, 670, 1735, 1846, 1976, 2044
Tru1I TTAA 3 cut(s) 1224, 1892, 1908
Tru9I TTAA 3 cut(s) 1224, 1892, 1908
TscAI CASTG 4 cut(s) 779, 910, 2027, 2307
TseFI GTSAC 2 cut(s) 697, 1697
TseI GCWGC 9 cut(s) 174, 463, 466, 833, 1030, 1589, 1868, 1952, 2228
Tsp45I GTSAC 2 cut(s) 697, 1697
TspDTI ATGAA 4 cut(s) 481, 771, 1115, 1968
TspGWI ACGGA 3 cut(s) 975, 1667, 2102
TspRI CASTG 4 cut(s) 779, 910, 2027, 2307
Tth111I GACNNNGTC 1 cut(s) 41
Van91I CCANNNNNTGG 1 cut(s) 234
VneI GTGCAC 2 cut(s) 808, 1285
VpaK11BI GGWCC 5 cut(s) 517, 635, 896, 1280, 1307
VspI ATTAAT 1 cut(s) 1892
XapI RAATTY 4 cut(s) 731, 1007, 1263, 1691
XceI RCATGY 1 cut(s) 1840
XmaJI CCTAGG 1 cut(s) 2132
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 2 cut(s) 1563, 1612
XspI CTAG 4 cut(s) 804, 845, 1524, 2133
ZrmI AGTACT 2 cut(s) 344, 1381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.