MD04G1022100.v1.1

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007064
Physical Location & Seq
Forward (+)
2600080 .. 2608054
7975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1022100.v1.1.491

Sequence Viewer

Length: 2322 bp
ATGTCGGAGATGGCGAACACAGACCCGGAAGGGATCGACGGAGTTCGGATGACGTGGAACGTCTGGCCGCGCACCAAGGTGGAGGCCAGCAAGTGCGTGATCCCACTGGCCGCCTGCATCTCCCCGATCCGCTCCCACCCTGACATCCCCACGCTCCCCTACGCGCCGCTCCGCTGCAAGACCTGCTCTGCCGCTCTCAACCCCTTTGCGCGCGTCGACTTCACCGCCAAGATCTGGATCTGCCCTTTCTGCTACCAGCGCAACCACTTCCCGCCTCACTACGCCGCGATCTCCGAGACCAATCTCCCCGGTGAGCTATACCCTCAGTACACCACCCTCCAATACGCTTTGCCGACGGATTCCGCTGCCCCTCAATCTCTGCCGTCCCCGGTCTTTCTCTTCGTGCTCGACACGTGTATGATCGAGGAGGAGATGGGATATGTCAAATCGGCGCTCAAAAGGGCAATTGGGTTGCTGCCCGATAATGCGCTTGTTGGGTTCGTTTCGTATGGGACCCAAGTTCAGGTCCATGAATTGGGGTTCTCTGATTTGTCGAAAGTGTATGTCTTTCGGGGGAGTAAGGAGATTTCAAAGGAACAGGTTTTGGAGCAATTGGGTCTCGGCAATTTGGGTCGGAGACCTGGTCCAATTGGTGGCGGAGGGTACCAGCTGAAGGGGGTACAAAATGGGCTTCCCAGTGCTGGTGTGACCCGGTTCTTGTTGCCCGCTTCGGATTGCGAATACACACTGAACACGTTGTTGGATGAATTGCAAACGGACCAGTGGCCTGTGCCGCAAGGAAGCCGTGCTTCCAGGTGCACAGGAGTGGCATTGAGTGTTGCTGCCGGGTTACTTGGAGCTTGTGTGCCTGGAACTGGGGCTCGCATTGTAGCTTTGGTTGGCGGTCCATGCACTGAAGGGCCTGGCGCGATTGTATCGAAAGATTTGTCAGACCCAGTACGCTCCCATAAGGATCTGGATAAGGATGCAGCACCATACTTTAAGAAAGCAATCAAATTTTATGAAAATCTTGCAAAGCAGCTGGTTAGCCAGGGGCATGTCTTGGACCTATTTGCATCTGCACTCGATCAGGTTGGGGTTGCAGAAATGAAAGTTGCAATTGAAAGAACTGGTGGGCTTGTTGTTCTGGCAGAAAGTTTTGGTCATTCTGTATTCAAAGACTCTTTCAAGCGTGTTTTTGAAGAAGGGGAACAGTCGCTTGGCCTTTGTTTCAATGGTATGCTTGAGATCAACTGTTCTAAGGACATAAAAATTCAAGGGATTATTGGACCTTGCACATCTTTGGAGAAGAAAGGACCTGCTGTTGCTGATACTGCTATAGGGGAGGGGAATACAACAGCTTGGAAAATGTGTGGCCTTGACAAGAGTACTTGCTTGACAGTTTTCTTTGATTTGTCATCTAGTGATCAGTCAAATACACCGGGAACTGTAAATCCACAATTGTATCTGCAGTTTCTTACAAGTTTTCAAAACCCTGAAGGTCAAATGATGCTTCGAGTAACAACAATTACTAGACAATGGATAGATAGTTCAGTTAGCTCAGAGGAATTGGTTCAAGGATTTGACCAAGAGACTGCTGCTGTAGTAATGGCAAGAAAAACTTCCTTGAAAATGGAGTCAGAGGAATCTTTTGATGCTACACGATGGTTGGATCGAGGACTTATTCGCCTCTGTTCGAAATTTGGTGATTATCGAAAAGATGATCCATCATCCTTTACTTTAAATCCCTCTTTCTCATTATTCCCTCAATTTATGTTTAACCTCCGACGCTCACAATTTGTGCAGGTTTTCAACAACAGCCCAGATGAAACAGCATATTTTCGCGTGTTGTTAAATCGGGAAAATATCAGCAATGCTGCTGTCATGATACAACCATCATTAATATCGTATTCATTTAATTCGCTGCCTCAGCCAGCATTGTTGGATGTGGCATCCATTGCAGCCGACCGTATTCTCTTGTTGGATTCATATTTTAGTATAGTCATTTTCCATGGAATGACAATAGCACAGTGGCGTAACATGGGATACCAGAATCAGCCAGAACACCAGGCATTTGCACAACTACTGCAAGCTCCCCACGATGATACACAATTGATTGTTCGGGACCGTTTCCCTGTCCCTAGATTGGTGGTGTGCGATCAGCATGGCTCCCAGGCAAGGTTCTTGTTAGCAAAGTTGAACCCGTCAGCAACATACAACAATGCGCATGAAATGGCTGCCGGTTCAGATGTTATCTTCACAGATGATGTAAGCCTTCAAGTTTTCTTTGAGCATCTTCAGAGGCTGGCAGTGCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

774

Amino Acids

84.97

Weight (kDa)

5.58

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 56 - 94 3.3e-16 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 129 - 400 4.7e-65 Sec23/Sec24 trunk domain
Sec23_BS PF08033 411 - 514 4e-23 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 529 - 626 2.1e-25 Sec23/Sec24 helical domain
Gelsolin PF00626 641 - 729 9.8e-16 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2226
Acc36I ACCTGC 3 cut(s) 191, 1327, 1795
Acc65I GGTACC 1 cut(s) 663
AccB1I GGYRCC 1 cut(s) 663
AccB7I CCANNNNNTGG 3 cut(s) 234, 535, 653
AccBSI CCGCTC 3 cut(s) 132, 169, 194
AccI GTMKAC 1 cut(s) 216
AccII CGCG 7 cut(s) 70, 164, 211, 213, 287, 929, 1845
AclWI GGATC 7 cut(s) 41, 94, 121, 245, 981, 1680, 1718
AcoI YGGCCR 2 cut(s) 65, 108
AcsI RAATTY 3 cut(s) 1016, 1272, 1700
AcuI CTGAAG 4 cut(s) 692, 936, 1518, 2282
AcvI CACGTG 1 cut(s) 414
AfaI GTAC 5 cut(s) 329, 665, 681, 960, 1390
AfiI CCNNNNNNNGG 9 cut(s) 234, 523, 535, 653, 673, 701, 875, 2146, 2178
AflIII ACRYGT 3 cut(s) 411, 413, 753
AjiI CACGTC 1 cut(s) 54
AjnI CCWGG 7 cut(s) 640, 812, 868, 922, 1050, 2067, 2172
AjuI GAANNNNNNNTTGG 4 cut(s) 743, 775, 1203, 1235
AleI CACNNNNGTG 2 cut(s) 77, 824
AloI GAACNNNNNNTCC 4 cut(s) 1438, 1470, 1534, 1566
AluBI AGCT 8 cut(s) 316, 670, 860, 893, 1042, 1361, 1560, 2093
AluI AGCT 8 cut(s) 316, 670, 860, 893, 1042, 1361, 1560, 2093
Alw21I GWGCWC 2 cut(s) 408, 821
Alw26I GTCTC 4 cut(s) 290, 623, 631, 1586
Alw44I GTGCAC 1 cut(s) 817
AlwI GGATC 7 cut(s) 41, 94, 121, 245, 981, 1680, 1718
AlwNI CAGNNNCTG 1 cut(s) 2305
AoxI GGCC 7 cut(s) 65, 84, 108, 785, 920, 1222, 1375
ApaLI GTGCAC 1 cut(s) 817
ApoI RAATTY 3 cut(s) 1016, 1272, 1700
ArsI GACNNNNNNTTYG 2 cut(s) 932, 964
AseI ATTAAT 1 cut(s) 1901
Asp700I GAANNNNTTC 2 cut(s) 1572, 1621
Asp718I GGTACC 1 cut(s) 663
AspLEI GCGC 9 cut(s) 72, 166, 211, 213, 261, 454, 490, 929, 2227
AsuC2I CCSGG 6 cut(s) 26, 309, 389, 712, 847, 1443
AsuHPI GGTGA 3 cut(s) 214, 323, 1718
AsuII TTCGAA 1 cut(s) 1697
AvaII GGWCC 9 cut(s) 513, 526, 644, 778, 905, 1066, 1289, 1316, 2125
BaeGI GKGCMC 1 cut(s) 821
BaeI ACNNNNGTAYC 2 cut(s) 1448, 1481
BanI GGYRCC 1 cut(s) 663
BanII GRGCYC 1 cut(s) 883
BbrPI CACGTG 1 cut(s) 414
Bbv12I GWGCWC 2 cut(s) 408, 821
BbvCI CCTCAGC 1 cut(s) 1929
BccI CCATC 5 cut(s) 4, 427, 1659, 1735, 1903
BceAI ACGGC 2 cut(s) 367, 789
BciT130I CCWGG 7 cut(s) 642, 814, 870, 924, 1052, 2069, 2174
BciVI GTATCC 1 cut(s) 2039
BclI TGATCA 1 cut(s) 1426
BcnI CCSGG 6 cut(s) 26, 309, 389, 712, 847, 1443
BcoDI GTCTC 4 cut(s) 290, 623, 631, 1586
BfaI CTAG 3 cut(s) 1422, 1533, 2142
BfmI CTRYAG 3 cut(s) 1338, 1469, 1602
BfoI RGCGCY 1 cut(s) 455
BfuAI ACCTGC 3 cut(s) 191, 1327, 1795
BfuI GTATCC 1 cut(s) 2039
BglII AGATCT 1 cut(s) 231
BmcAI AGTACT 1 cut(s) 1390
Bme18I GGWCC 9 cut(s) 513, 526, 644, 778, 905, 1066, 1289, 1316, 2125
BmgBI CACGTC 1 cut(s) 54
BmiI GGNNCC 5 cut(s) 514, 515, 665, 2126, 2170
BmrI ACTGGG 3 cut(s) 690, 885, 950
BmsI GCATC 7 cut(s) 126, 976, 1085, 1500, 1645, 1961, 2302
BmuI ACTGGG 3 cut(s) 690, 885, 950
Bpu10I CCTNAGC 1 cut(s) 1929
Bpu14I TTCGAA 1 cut(s) 1697
BpuEI CTTGAG 1 cut(s) 1265
BpuMI CCSGG 6 cut(s) 26, 309, 389, 712, 847, 1443
BsaAI YACGTR 1 cut(s) 414
BsaBI GATNNNNATC 1 cut(s) 236
BsaI GGTCTC 3 cut(s) 290, 623, 631
BsaJI CCNNGG 6 cut(s) 75, 307, 387, 1051, 2011, 2172
BsaXI ACNNNNNCTCC 4 cut(s) 628, 658, 849, 879
Bsc4I CCNNNNNNNGG 9 cut(s) 234, 523, 535, 653, 673, 701, 875, 2146, 2178
Bse118I RCCGGY 1 cut(s) 2240
Bse1I ACTGG 6 cut(s) 111, 696, 781, 880, 956, 1135
Bse3DI GCAATG 2 cut(s) 1879, 1956
Bse8I GATNNNNATC 1 cut(s) 236
BseBI CCWGG 7 cut(s) 642, 814, 870, 924, 1052, 2069, 2174
BseDI CCNNGG 6 cut(s) 75, 307, 387, 1051, 2011, 2172
BseGI GGATG 7 cut(s) 54, 144, 769, 991, 1730, 1951, 1952
BseJI GATNNNNATC 1 cut(s) 236
BseLI CCNNNNNNNGG 9 cut(s) 234, 523, 535, 653, 673, 701, 875, 2146, 2178
BseMI GCAATG 2 cut(s) 1879, 1956
BseMII CTCAG 3 cut(s) 338, 1575, 1943
BseNI ACTGG 6 cut(s) 111, 696, 781, 880, 956, 1135
BsePI GCGCGC 1 cut(s) 209
BseRI GAGGAG 2 cut(s) 440, 443
BseSI GKGCMC 1 cut(s) 821
BsgI GTGCAG 2 cut(s) 1065, 1823
Bsh1236I CGCG 7 cut(s) 70, 164, 211, 213, 287, 929, 1845
Bsh1285I CGRYCG 1 cut(s) 1969
BshFI GGCC 7 cut(s) 67, 86, 110, 787, 922, 1224, 1377
BshNI GGYRCC 1 cut(s) 663
BsiEI CGRYCG 1 cut(s) 1969
BsiHKAI GWGCWC 2 cut(s) 408, 821
BsiSI CCGG 7 cut(s) 26, 309, 389, 712, 846, 1442, 2241
BslFI GGGAC 4 cut(s) 370, 526, 2123, 2138
BslI CCNNNNNNNGG 9 cut(s) 234, 523, 535, 653, 673, 701, 875, 2146, 2178
BsmAI GTCTC 4 cut(s) 290, 623, 631, 1586
BsmFI GGGAC 4 cut(s) 370, 526, 2123, 2138
BsnI GGCC 7 cut(s) 67, 86, 110, 787, 922, 1224, 1377
Bso31I GGTCTC 3 cut(s) 290, 623, 631
Bsp119I TTCGAA 1 cut(s) 1697
Bsp1286I GDGCHC 3 cut(s) 408, 821, 883
Bsp19I CCATGG 1 cut(s) 2011
BspANI GGCC 7 cut(s) 67, 86, 110, 787, 922, 1224, 1377
BspCNI CTCAG 3 cut(s) 337, 1574, 1942
BspFNI CGCG 7 cut(s) 70, 164, 211, 213, 287, 929, 1845
BspHI TCATGA 1 cut(s) 1884
BspLI GGNNCC 5 cut(s) 514, 515, 665, 2126, 2170
BspMAI CTGCAG 1 cut(s) 1473
BspMI ACCTGC 3 cut(s) 191, 1327, 1795
BspPI GGATC 7 cut(s) 41, 94, 121, 245, 981, 1680, 1718
BspT104I TTCGAA 1 cut(s) 1697
BspT107I GGYRCC 1 cut(s) 663
BspTNI GGTCTC 3 cut(s) 290, 623, 631
BsrBI CCGCTC 3 cut(s) 132, 169, 194
BsrDI GCAATG 2 cut(s) 1879, 1956
BsrFI RCCGGY 1 cut(s) 2240
BsrI ACTGG 6 cut(s) 111, 696, 781, 880, 956, 1135
BssAI RCCGGY 1 cut(s) 2240
BssECI CCNNGG 6 cut(s) 75, 307, 387, 1051, 2011, 2172
BssHII GCGCGC 1 cut(s) 209
BssT1I CCWWGG 2 cut(s) 75, 2011
Bst2UI CCWGG 7 cut(s) 642, 814, 870, 924, 1052, 2069, 2174
Bst4CI ACNGT 7 cut(s) 1215, 1256, 1402, 1450, 1970, 2031, 2129
Bst6I CTCTTC 1 cut(s) 404
BstAPI GCANNNNNTGC 2 cut(s) 183, 1958
BstBAI YACGTR 1 cut(s) 414
BstBI TTCGAA 1 cut(s) 1697
BstC8I GCNNGC 8 cut(s) 88, 115, 211, 726, 883, 1935, 2091, 2307
BstDEI CTNAG 4 cut(s) 324, 1260, 1561, 1929
BstDSI CCRYGG 1 cut(s) 2011
BstF5I GGATG 7 cut(s) 54, 144, 769, 991, 1730, 1951, 1952
BstFNI CGCG 7 cut(s) 70, 164, 211, 213, 287, 929, 1845
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 9 cut(s) 72, 166, 211, 213, 261, 454, 490, 929, 2227
BstMAI GTCTC 4 cut(s) 290, 623, 631, 1586
BstMCI CGRYCG 1 cut(s) 1969
BstMWI GCNNNNNNNGC 9 cut(s) 183, 249, 258, 793, 909, 1334, 1930, 1958, 2311
BstNI CCWGG 7 cut(s) 642, 814, 870, 924, 1052, 2069, 2174
BstNSI RCATGY 1 cut(s) 1061
BstSFI CTRYAG 3 cut(s) 1338, 1469, 1602
BstSLI GKGCMC 1 cut(s) 821
BstUI CGCG 7 cut(s) 70, 164, 211, 213, 287, 929, 1845
BstX2I RGATCY 3 cut(s) 231, 237, 973
BstYI RGATCY 3 cut(s) 231, 237, 973
BsuI GTATCC 1 cut(s) 2039
BsuRI GGCC 7 cut(s) 67, 86, 110, 787, 922, 1224, 1377
BtgI CCRYGG 1 cut(s) 2011
BtrI CACGTC 1 cut(s) 54
BtsCI GGATG 7 cut(s) 54, 144, 769, 991, 1730, 1951, 1952
BtsI GCAGTG 1 cut(s) 2316
BtsIMutI CAGTG 7 cut(s) 104, 703, 746, 788, 912, 2036, 2316
BveI ACCTGC 3 cut(s) 191, 1327, 1795
Cac8I GCNNGC 8 cut(s) 88, 115, 211, 726, 883, 1935, 2091, 2307
CaiI CAGNNNCTG 1 cut(s) 2305
CciI TCATGA 1 cut(s) 1884
CfoI GCGC 9 cut(s) 72, 166, 211, 213, 261, 454, 490, 929, 2227
Cfr10I RCCGGY 1 cut(s) 2240
CseI GACGC 2 cut(s) 202, 1797
CsiI ACCWGGT 1 cut(s) 640
Csp6I GTAC 5 cut(s) 328, 664, 680, 959, 1389
CviAII CATG 8 cut(s) 530, 909, 1058, 1885, 2012, 2041, 2165, 2228
CviQI GTAC 5 cut(s) 328, 664, 680, 959, 1389
DdeI CTNAG 4 cut(s) 324, 1260, 1561, 1929
DraI TTTAAA 1 cut(s) 1743
EaeI YGGCCR 2 cut(s) 65, 108
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
EciI GGCGGA 1 cut(s) 672
Eco130I CCWWGG 2 cut(s) 75, 2011
Eco24I GRGCYC 1 cut(s) 883
Eco31I GGTCTC 3 cut(s) 290, 623, 631
Eco47I GGWCC 9 cut(s) 513, 526, 644, 778, 905, 1066, 1289, 1316, 2125
Eco57I CTGAAG 4 cut(s) 692, 936, 1518, 2282
Eco72I CACGTG 1 cut(s) 414
EcoO109I RGGNCCY 3 cut(s) 513, 920, 1316
EcoRII CCWGG 7 cut(s) 640, 812, 868, 922, 1050, 2067, 2172
EcoT14I CCWWGG 2 cut(s) 75, 2011
EcoT38I GRGCYC 1 cut(s) 883
ErhI CCWWGG 2 cut(s) 75, 2011
FaeI CATG 8 cut(s) 533, 912, 1061, 1888, 2015, 2044, 2168, 2231
FalI AAGNNNNNCTT 4 cut(s) 793, 825, 1606, 1638
FaqI GGGAC 4 cut(s) 370, 526, 2123, 2138
FatI CATG 8 cut(s) 529, 908, 1057, 1884, 2011, 2040, 2164, 2227
FauI CCCGC 2 cut(s) 279, 733
FbaI TGATCA 1 cut(s) 1426
FblI GTMKAC 1 cut(s) 216
FokI GGATG 7 cut(s) 61, 131, 776, 998, 1717, 1939, 1958
FriOI GRGCYC 1 cut(s) 883
FspAI RTGCGCAY 1 cut(s) 2226
FspBI CTAG 3 cut(s) 1422, 1533, 2142
FspI TGCGCA 1 cut(s) 2226
GlaI GCGC 9 cut(s) 71, 165, 210, 212, 260, 453, 489, 928, 2226
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 7 cut(s) 67, 86, 110, 787, 922, 1224, 1377
HapII CCGG 7 cut(s) 26, 309, 389, 712, 846, 1442, 2241
HgaI GACGC 2 cut(s) 202, 1797
HhaI GCGC 9 cut(s) 72, 166, 211, 213, 261, 454, 490, 929, 2227
Hin1II CATG 8 cut(s) 533, 912, 1061, 1888, 2015, 2044, 2168, 2231
Hin6I GCGC 9 cut(s) 70, 164, 209, 211, 259, 452, 488, 927, 2225
HinP1I GCGC 9 cut(s) 70, 164, 209, 211, 259, 452, 488, 927, 2225
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 6 cut(s) 359, 1181, 1637, 1646, 1985, 2053
HpaII CCGG 7 cut(s) 26, 309, 389, 712, 846, 1442, 2241
HphI GGTGA 3 cut(s) 214, 323, 1718
Hpy166II GTNNAC 3 cut(s) 217, 330, 819
Hpy188III TCNNGA 6 cut(s) 235, 977, 1859, 1885, 2123, 2319
Hpy8I GTNNAC 3 cut(s) 217, 330, 819
Hpy99I CGWCG 4 cut(s) 41, 218, 358, 1791
HpyAV CCTTC 6 cut(s) 23, 667, 911, 1199, 1493, 2285
HpyCH4III ACNGT 7 cut(s) 1215, 1256, 1402, 1450, 1970, 2031, 2129
HpyCH4IV ACGT 4 cut(s) 53, 60, 413, 755
HpyF10VI GCNNNNNNNGC 9 cut(s) 183, 249, 258, 793, 909, 1334, 1930, 1958, 2311
HpyF3I CTNAG 4 cut(s) 324, 1260, 1561, 1929
HpySE526I ACGT 4 cut(s) 53, 60, 413, 755
Hsp92II CATG 8 cut(s) 533, 912, 1061, 1888, 2015, 2044, 2168, 2231
HspAI GCGC 9 cut(s) 70, 164, 209, 211, 259, 452, 488, 927, 2225
KflI GGGWCCC 1 cut(s) 513
KpnI GGTACC 1 cut(s) 667
Ksp22I TGATCA 1 cut(s) 1426
LmnI GCTCC 8 cut(s) 137, 159, 174, 607, 857, 968, 2098, 2174
LweI GCATC 7 cut(s) 126, 976, 1085, 1500, 1645, 1961, 2302
MabI ACCWGGT 1 cut(s) 640
MaeI CTAG 3 cut(s) 1422, 1533, 2142
MaeII ACGT 4 cut(s) 53, 60, 413, 755
MaeIII GTNAC 4 cut(s) 706, 849, 1519, 2036
MbiI CCGCTC 3 cut(s) 132, 169, 194
MboII GAAGA 5 cut(s) 391, 1214, 1321, 2248, 2288
MfeI CAATTG 6 cut(s) 465, 611, 648, 1119, 1460, 2111
MflI RGATCY 3 cut(s) 231, 237, 973
MhlI GDGCHC 3 cut(s) 408, 821, 883
MlyI GAGTC 2 cut(s) 1175, 1646
MmeI TCCRAC 6 cut(s) 614, 741, 1650, 1810, 1923, 1962
MroXI GAANNNNTTC 2 cut(s) 1572, 1621
MseI TTAA 6 cut(s) 1002, 1742, 1779, 1853, 1901, 1917
MslI CAYNNNNRTG 3 cut(s) 77, 416, 824
MspA1I CMGCKG 4 cut(s) 174, 365, 670, 1042
MspI CCGG 7 cut(s) 26, 309, 389, 712, 846, 1442, 2241
MunI CAATTG 6 cut(s) 465, 611, 648, 1119, 1460, 2111
MvaI CCWGG 7 cut(s) 642, 814, 870, 924, 1052, 2069, 2174
MvnI CGCG 7 cut(s) 70, 164, 211, 213, 287, 929, 1845
MwoI GCNNNNNNNGC 9 cut(s) 183, 249, 258, 793, 909, 1334, 1930, 1958, 2311
NciI CCSGG 6 cut(s) 26, 309, 389, 712, 847, 1443
NcoI CCATGG 1 cut(s) 2011
NlaIII CATG 8 cut(s) 533, 912, 1061, 1888, 2015, 2044, 2168, 2231
NlaIV GGNNCC 5 cut(s) 514, 515, 665, 2126, 2170
NmeAIII GCCGAG 1 cut(s) 600
NmuCI GTSAC 1 cut(s) 706
NsbI TGCGCA 1 cut(s) 2226
NspI RCATGY 1 cut(s) 1061
NspV TTCGAA 1 cut(s) 1697
OliI CACNNNNGTG 2 cut(s) 77, 824
PagI TCATGA 1 cut(s) 1884
PauI GCGCGC 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdmI GAANNNNTTC 2 cut(s) 1572, 1621
PfeI GAWTC 4 cut(s) 359, 1646, 1985, 2053
PflFI GACNNNGTC 1 cut(s) 642
PflMI CCANNNNNTGG 3 cut(s) 234, 535, 653
PleI GAGTC 2 cut(s) 1175, 1645
PmaCI CACGTG 1 cut(s) 414
PmlI CACGTG 1 cut(s) 414
PpsI GAGTC 2 cut(s) 1175, 1645
Ppu21I YACGTR 1 cut(s) 414
PpuMI RGGWCCY 2 cut(s) 513, 1316
PshBI ATTAAT 1 cut(s) 1901
Psp5II RGGWCCY 2 cut(s) 513, 1316
Psp6I CCWGG 7 cut(s) 640, 812, 868, 922, 1050, 2067, 2172
PspCI CACGTG 1 cut(s) 414
PspGI CCWGG 7 cut(s) 640, 812, 868, 922, 1050, 2067, 2172
PspN4I GGNNCC 5 cut(s) 514, 515, 665, 2126, 2170
PspPPI RGGWCCY 2 cut(s) 513, 1316
PstI CTGCAG 1 cut(s) 1473
PstNI CAGNNNCTG 1 cut(s) 2305
PsuI RGATCY 3 cut(s) 231, 237, 973
PsyI GACNNNGTC 1 cut(s) 642
PteI GCGCGC 1 cut(s) 209
PvuII CAGCTG 2 cut(s) 670, 1042
RsaI GTAC 5 cut(s) 329, 665, 681, 960, 1390
RsaNI GTAC 5 cut(s) 328, 664, 680, 959, 1389
RseI CAYNNNNRTG 3 cut(s) 77, 416, 824
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 6 cut(s) 1002, 1742, 1779, 1853, 1901, 1917
ScaI AGTACT 1 cut(s) 1390
SchI GAGTC 2 cut(s) 1175, 1646
SduI GDGCHC 3 cut(s) 408, 821, 883
SexAI ACCWGGT 1 cut(s) 640
SfaNI GCATC 7 cut(s) 126, 976, 1085, 1500, 1645, 1961, 2302
SfcI CTRYAG 3 cut(s) 1338, 1469, 1602
SfuI TTCGAA 1 cut(s) 1697
SinI GGWCC 9 cut(s) 513, 526, 644, 778, 905, 1066, 1289, 1316, 2125
SmiMI CAYNNNNRTG 3 cut(s) 77, 416, 824
SmlI CTYRAG 1 cut(s) 1244
SmoI CTYRAG 1 cut(s) 1244
SspMI CTAG 3 cut(s) 1422, 1533, 2142
StyI CCWWGG 2 cut(s) 75, 2011
TaaI ACNGT 7 cut(s) 1215, 1256, 1402, 1450, 1970, 2031, 2129
TaiI ACGT 4 cut(s) 56, 63, 416, 758
TatI WGTACW 2 cut(s) 327, 1388
TauI GCSGC 6 cut(s) 70, 113, 169, 194, 287, 796
TfiI GAWTC 4 cut(s) 359, 1646, 1985, 2053
Tru1I TTAA 6 cut(s) 1002, 1742, 1779, 1853, 1901, 1917
Tru9I TTAA 6 cut(s) 1002, 1742, 1779, 1853, 1901, 1917
TscAI CASTG 7 cut(s) 111, 703, 753, 788, 919, 2036, 2316
TseFI GTSAC 1 cut(s) 706
Tsp45I GTSAC 1 cut(s) 706
TspDTI ATGAA 8 cut(s) 546, 780, 1038, 1124, 1842, 1902, 1977, 2244
TspGWI ACGGA 3 cut(s) 54, 371, 791
TspRI CASTG 7 cut(s) 111, 703, 753, 788, 919, 2036, 2316
Tth111I GACNNNGTC 1 cut(s) 642
Van91I CCANNNNNTGG 3 cut(s) 234, 535, 653
VneI GTGCAC 1 cut(s) 817
VpaK11BI GGWCC 9 cut(s) 513, 526, 644, 778, 905, 1066, 1289, 1316, 2125
VspI ATTAAT 1 cut(s) 1901
XapI RAATTY 3 cut(s) 1016, 1272, 1700
XceI RCATGY 1 cut(s) 1061
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 2 cut(s) 1572, 1621
XspI CTAG 3 cut(s) 1422, 1533, 2142
ZrmI AGTACT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.