MD14G1124000.v1.1

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007064
Physical Location & Seq
Reverse (-)
19946559 .. 19953952
7394 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1124000.v1.1.491

Sequence Viewer

Length: 2322 bp
ATGTCGGAGATGGCGAACACAGACCCGGAAGGGATCGACGGAGTTCGGATGAGCTGGAACGTCTGGCCACGCACCAAGGTGGAGGCCAGCAAGTGCGTGATCCCACTGGCCGCCTGCATCTCCCCGATCCGGTCACACCCCGACATCCCCACGCTCCCCTACGCGCCGCTCCGCTGCAAGACCTGCTCTGCCGTGCTGAACCCCTTTGCGCGCGTCGACTTCACCGCCAAGATCTGGATCTGCCCCTTCTGCTACCAGCGCAATCACTTCCCGCCTCACTACGCCGCGATCTCTGAGACCAATCTCCCTGGTGAGCTCTACCCTCAGTACACCACCCTCCAATACGCTCTGCCGACTGATTCCGCCGCCCCTCAATCTCTGCCGTCCCCGGTTTTTGTCTTCGTGCTCGACACGTGTATGATCGAGGAGGAGATGGAATATGTCAAATCGGCGCTCAAAAGGGCAATTGGGTTGCTGCCCGATAATGCGCTTGTTGGGTTCGTTTCGTATGGAACCCAAGTTCAGGTCCATGAATTGGGGTTCTCTGATTTGTCGAAAGTGTATGTCTTTCGGGGTAGTAAGGAGATTTCAAAGGAACAAGTTTTGGAGCAATTGGGTCTCAGCGTTCTTGGTCGGAGACCTGGTCCAATTGGTGGCGGAGGGTACCAGCCGAAGGGGGTGCAAATGGGAATTCCCAATGCTGGTGTAACCCGCTTTTTGTTGCCTGCTTCGGATTGCGAATACACACTGAACACGCTGTTGGATGAATTGCAAACGGACCAGTGGCCTGTGCCACAAGGAAGTCGTGCTTCCAGGTGCACAGGAGTGGCGTTGAGTGTTGCTGCAGGGTTACTTGGAGCTTGTCTACCTGGAACTGGGGCTCACATTGTAGCTTTGGTTGGCGGTCCATGCACTGAGGGGCCTGGCGCGATTGTATCGAAAGATTTGTCAGACCCAGTACGTTCCCATAAAGATCTAGATAAGGATGCGGCACCGTACTTTAAGAAAGCAATCAAGTTTTATGAAAATCTTGCAAAACAGCTGGTTAGCCAAGGCCATGTCTTAGACCTGTTTGCATCTGCACTCGATCAGGTTGGGGTTGCAGAAATGAAAGTTGCAATTGAAAGAACTGGTGGGCTTGTTGTTCTAGCAGAAAGTTTTGGTCATTCTGTATTCAAAGATTCTTTCAAGCGTGTTTTTGAAGAAGGGGAACAATCGCTTGGCCTATGTTTCAATGGTACACTTGAGATCAACTGTTCGAAGGACATAAAAATTCAGGGGGTCATTGGACCCTGCACATCTTTGGAGAAGAAAGGACCTGCTGTTGCTGATACTGCTATAGGGGAGGGAAATACAACAGCTTGGAAAATGTGTGGCCTTGACAGGAGTACTTGCTTGACAGTTTTCTTTGATCTCTCATCTAGTGATCAGTCAAATACACCAGGAACTGTAAATCCACAATTGTATCTGCAGTTTCTTACAAGCTTTCAAAACCCTGAAGGTCAAATGATGCTTCGAGTAACAACAATCACTAGACGATGGATAGATAGTTCCGTTAGCCCAGAGGAATTGGTTCAAGGATTTGACCAAGAGACTGCTGCTGTAGTGATGGCAAGAAAAACTTCCTTGAAAATGGAGTCAGAGGAATCTTTTGATGCTACACGATGGTTGGATCGAGGACTTATTCGCCTCTGTTCGAAATTTGGTGACTATCGAAAAGATGATCCATCATCCTTTACTTTAAATCCCTGTTTCTCATTATTCCCTCAATTTATGTTTAACCTCCGACGCTCACAATTTGTGCAGGTTTTCAACAACAGCCCAGATGAAACTGCATATTTTCGCATGTTGTTAAATCGGGAAAATATCAGCAATGCTGCTGTCATGATACAACCATCATTAATATCATATTCATTTAATTCGCTGCCTCAGCCAGCATTGTTGGATGTGGCATCCATTGCAGCTGACCGTATTCTCTTGTTGGATTCATATTTTAGTATAGTCATTTTCCATGGAATGACAATAGCACAGTGGCGTAACATGGGATACCAGAATCAGCCAGAACACCAGGCATTTGCACAACTACTGCAAGCTCCTCATGATGATACACAATTGATTGTTCGGGACCGTTTCCCTGTCCCTAGATTGGTGGTGTGCGATCAGCATGGTTCCCAGGCAAGATTCTTGTTAGCAAAGTTAAACCCATCAGCAACATACAACAATGCCCATGAAATGGCCGCCGGTTCAGATGTAATCTTCACGGACGATGTGAGCCTTCAAGTTTTCTTTGAGCATCTTCAGAGGCTGGCTGTACAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

774

Amino Acids

85.15

Weight (kDa)

5.57

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 56 - 94 8.1e-17 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 129 - 400 7.6e-65 Sec23/Sec24 trunk domain
Sec23_BS PF08033 411 - 514 2.5e-23 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 529 - 626 4.3e-26 Sec23/Sec24 helical domain
Gelsolin PF00626 641 - 729 9.8e-16 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 191, 1327, 1795
Acc65I GGTACC 1 cut(s) 663
AccB1I GGYRCC 2 cut(s) 663, 991
AccB7I CCANNNNNTGG 4 cut(s) 234, 535, 653, 2233
AccBSI CCGCTC 1 cut(s) 169
AccI GTMKAC 2 cut(s) 216, 865
AccII CGCG 5 cut(s) 164, 211, 213, 287, 929
AclWI GGATC 6 cut(s) 41, 94, 121, 245, 1680, 1718
AcoI YGGCCR 3 cut(s) 65, 108, 2235
AcsI RAATTY 3 cut(s) 690, 1272, 1700
AcuI CTGAAG 2 cut(s) 1518, 2282
AcvI CACGTG 1 cut(s) 414
AfaI GTAC 7 cut(s) 329, 665, 960, 998, 1240, 1390, 2313
AflIII ACRYGT 2 cut(s) 411, 413
AjnI CCWGG 8 cut(s) 307, 640, 812, 868, 922, 1441, 2067, 2172
AjuI GAANNNNNNNTTGG 4 cut(s) 743, 775, 1203, 1235
AleI CACNNNNGTG 2 cut(s) 77, 824
AloI GAACNNNNNNTCC 6 cut(s) 504, 536, 1438, 1470, 1534, 1566
AluBI AGCT 9 cut(s) 54, 316, 860, 893, 1042, 1361, 1485, 1964, 2093
AluI AGCT 9 cut(s) 54, 316, 860, 893, 1042, 1361, 1485, 1964, 2093
Alw21I GWGCWC 3 cut(s) 318, 408, 821
Alw26I GTCTC 4 cut(s) 290, 623, 631, 1586
Alw44I GTGCAC 1 cut(s) 817
AlwI GGATC 6 cut(s) 41, 94, 121, 245, 1680, 1718
AlwNI CAGNNNCTG 2 cut(s) 1448, 2305
AoxI GGCC 9 cut(s) 65, 84, 108, 785, 920, 1054, 1222, 1375, 2235
ApaLI GTGCAC 1 cut(s) 817
ApeKI GCWGC 7 cut(s) 174, 475, 842, 1598, 1877, 1924, 1961
ApoI RAATTY 3 cut(s) 690, 1272, 1700
ArsI GACNNNNNNTTYG 2 cut(s) 932, 964
AseI ATTAAT 1 cut(s) 1901
Asp700I GAANNNNTTC 2 cut(s) 1572, 1621
Asp718I GGTACC 1 cut(s) 663
AspLEI GCGC 7 cut(s) 166, 211, 213, 261, 454, 490, 929
AspS9I GGNCC 8 cut(s) 526, 644, 778, 905, 920, 1289, 1316, 2125
AsuC2I CCSGG 2 cut(s) 26, 389
AsuHPI GGTGA 3 cut(s) 214, 323, 1718
AsuII TTCGAA 2 cut(s) 1259, 1697
AvaII GGWCC 7 cut(s) 526, 644, 778, 905, 1289, 1316, 2125
BaeGI GKGCMC 1 cut(s) 821
BaeI ACNNNNGTAYC 2 cut(s) 1448, 1481
BalI TGGCCA 1 cut(s) 67
BanI GGYRCC 2 cut(s) 663, 991
BanII GRGCYC 2 cut(s) 318, 883
BbrPI CACGTG 1 cut(s) 414
BbsI GAAGAC 1 cut(s) 391
Bbv12I GWGCWC 3 cut(s) 318, 408, 821
BbvCI CCTCAGC 1 cut(s) 1929
BbvI GCAGC 7 cut(s) 161, 462, 829, 1585, 1864, 1911, 1973
BccI CCATC 8 cut(s) 4, 427, 1533, 1603, 1659, 1735, 1903, 2212
BceAI ACGGC 2 cut(s) 176, 367
BcgI CGANNNNNNTGC 2 cut(s) 661, 695
BciT130I CCWGG 8 cut(s) 309, 642, 814, 870, 924, 1443, 2069, 2174
BciVI GTATCC 1 cut(s) 2039
BclI TGATCA 1 cut(s) 1426
BcnI CCSGG 2 cut(s) 26, 389
BcoDI GTCTC 4 cut(s) 290, 623, 631, 1586
BfaI CTAG 5 cut(s) 977, 1148, 1422, 1533, 2142
BfmI CTRYAG 4 cut(s) 843, 1338, 1469, 1602
BfoI RGCGCY 1 cut(s) 455
BfuAI ACCTGC 3 cut(s) 191, 1327, 1795
BfuI GTATCC 1 cut(s) 2039
BglII AGATCT 2 cut(s) 231, 973
BmcAI AGTACT 1 cut(s) 1390
Bme18I GGWCC 7 cut(s) 526, 644, 778, 905, 1289, 1316, 2125
BmgT120I GGNCC 8 cut(s) 526, 644, 778, 905, 920, 1289, 1316, 2125
BmiI GGNNCC 7 cut(s) 514, 665, 921, 993, 1291, 2126, 2170
BmrI ACTGGG 2 cut(s) 885, 950
BmsI GCATC 7 cut(s) 126, 976, 1085, 1500, 1645, 1961, 2302
BmuI ACTGGG 2 cut(s) 885, 950
BpiI GAAGAC 1 cut(s) 391
Bpu10I CCTNAGC 1 cut(s) 1929
Bpu14I TTCGAA 2 cut(s) 1259, 1697
BpuEI CTTGAG 1 cut(s) 1265
BpuMI CCSGG 2 cut(s) 26, 389
BsaAI YACGTR 1 cut(s) 414
BsaBI GATNNNNATC 1 cut(s) 236
BsaI GGTCTC 3 cut(s) 290, 623, 631
BsaJI CCNNGG 6 cut(s) 75, 307, 387, 1051, 2011, 2172
BsaWI WCCGGW 1 cut(s) 129
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bse118I RCCGGY 1 cut(s) 2240
Bse1I ACTGG 5 cut(s) 111, 781, 880, 956, 1135
Bse3DI GCAATG 2 cut(s) 1879, 1956
Bse8I GATNNNNATC 1 cut(s) 236
BseBI CCWGG 8 cut(s) 309, 642, 814, 870, 924, 1443, 2069, 2174
BseDI CCNNGG 6 cut(s) 75, 307, 387, 1051, 2011, 2172
BseGI GGATG 7 cut(s) 54, 144, 769, 991, 1730, 1951, 1952
BseJI GATNNNNATC 1 cut(s) 236
BseMI GCAATG 2 cut(s) 1879, 1956
BseMII CTCAG 5 cut(s) 285, 338, 634, 906, 1943
BseNI ACTGG 5 cut(s) 111, 781, 880, 956, 1135
BsePI GCGCGC 1 cut(s) 209
BseRI GAGGAG 3 cut(s) 440, 443, 2085
BseSI GKGCMC 1 cut(s) 821
BseXI GCAGC 7 cut(s) 161, 462, 829, 1585, 1864, 1911, 1973
BsgI GTGCAG 3 cut(s) 1065, 1279, 1823
Bsh1236I CGCG 5 cut(s) 164, 211, 213, 287, 929
BshFI GGCC 9 cut(s) 67, 86, 110, 787, 922, 1056, 1224, 1377, 2237
BshNI GGYRCC 2 cut(s) 663, 991
BsiHKAI GWGCWC 3 cut(s) 318, 408, 821
BsiSI CCGG 4 cut(s) 26, 130, 389, 2241
BslFI GGGAC 3 cut(s) 370, 2123, 2138
BsmAI GTCTC 4 cut(s) 290, 623, 631, 1586
BsmFI GGGAC 3 cut(s) 370, 2123, 2138
BsnI GGCC 9 cut(s) 67, 86, 110, 787, 922, 1056, 1224, 1377, 2237
Bso31I GGTCTC 3 cut(s) 290, 623, 631
Bsp119I TTCGAA 2 cut(s) 1259, 1697
Bsp1286I GDGCHC 4 cut(s) 318, 408, 821, 883
Bsp1407I TGTACA 1 cut(s) 2311
Bsp19I CCATGG 1 cut(s) 2011
BspANI GGCC 9 cut(s) 67, 86, 110, 787, 922, 1056, 1224, 1377, 2237
BspCNI CTCAG 5 cut(s) 286, 337, 633, 907, 1942
BspFNI CGCG 5 cut(s) 164, 211, 213, 287, 929
BspHI TCATGA 2 cut(s) 1884, 2098
BspLI GGNNCC 7 cut(s) 514, 665, 921, 993, 1291, 2126, 2170
BspMAI CTGCAG 2 cut(s) 847, 1473
BspMI ACCTGC 3 cut(s) 191, 1327, 1795
BspPI GGATC 6 cut(s) 41, 94, 121, 245, 1680, 1718
BspT104I TTCGAA 2 cut(s) 1259, 1697
BspT107I GGYRCC 2 cut(s) 663, 991
BspTNI GGTCTC 3 cut(s) 290, 623, 631
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 2 cut(s) 1879, 1956
BsrFI RCCGGY 1 cut(s) 2240
BsrGI TGTACA 1 cut(s) 2311
BsrI ACTGG 5 cut(s) 111, 781, 880, 956, 1135
BssAI RCCGGY 1 cut(s) 2240
BssECI CCNNGG 6 cut(s) 75, 307, 387, 1051, 2011, 2172
BssHII GCGCGC 1 cut(s) 209
BssT1I CCWWGG 3 cut(s) 75, 1051, 2011
Bst2UI CCWGG 8 cut(s) 309, 642, 814, 870, 924, 1443, 2069, 2174
Bst4CI ACNGT 7 cut(s) 996, 1256, 1402, 1450, 1970, 2031, 2129
BstAPI GCANNNNNTGC 2 cut(s) 183, 1958
BstAUI TGTACA 1 cut(s) 2311
BstBAI YACGTR 1 cut(s) 414
BstBI TTCGAA 2 cut(s) 1259, 1697
BstC8I GCNNGC 7 cut(s) 88, 115, 211, 726, 1935, 2091, 2307
BstDEI CTNAG 6 cut(s) 294, 324, 620, 915, 1063, 1929
BstDSI CCRYGG 1 cut(s) 2011
BstF5I GGATG 7 cut(s) 54, 144, 769, 991, 1730, 1951, 1952
BstFNI CGCG 5 cut(s) 164, 211, 213, 287, 929
BstH2I RGCGCY 1 cut(s) 455
BstHHI GCGC 7 cut(s) 166, 211, 213, 261, 454, 490, 929
BstMAI GTCTC 4 cut(s) 290, 623, 631, 1586
BstMWI GCNNNNNNNGC 7 cut(s) 183, 249, 258, 909, 1334, 1930, 1958
BstNI CCWGG 8 cut(s) 309, 642, 814, 870, 924, 1443, 2069, 2174
BstNSI RCATGY 1 cut(s) 1849
BstSFI CTRYAG 4 cut(s) 843, 1338, 1469, 1602
BstSLI GKGCMC 1 cut(s) 821
BstUI CGCG 5 cut(s) 164, 211, 213, 287, 929
BstV1I GCAGC 7 cut(s) 161, 462, 829, 1585, 1864, 1911, 1973
BstV2I GAAGAC 1 cut(s) 391
BstX2I RGATCY 3 cut(s) 231, 237, 973
BstYI RGATCY 3 cut(s) 231, 237, 973
BsuI GTATCC 1 cut(s) 2039
BsuRI GGCC 9 cut(s) 67, 86, 110, 787, 922, 1056, 1224, 1377, 2237
BtgI CCRYGG 1 cut(s) 2011
BtsCI GGATG 7 cut(s) 54, 144, 769, 991, 1730, 1951, 1952
BtsIMutI CAGTG 5 cut(s) 104, 746, 788, 912, 2036
BveI ACCTGC 3 cut(s) 191, 1327, 1795
Cac8I GCNNGC 7 cut(s) 88, 115, 211, 726, 1935, 2091, 2307
CaiI CAGNNNCTG 2 cut(s) 1448, 2305
CciI TCATGA 2 cut(s) 1884, 2098
CfoI GCGC 7 cut(s) 166, 211, 213, 261, 454, 490, 929
Cfr10I RCCGGY 1 cut(s) 2240
Cfr13I GGNCC 8 cut(s) 526, 644, 778, 905, 920, 1289, 1316, 2125
CseI GACGC 2 cut(s) 202, 1797
CsiI ACCWGGT 1 cut(s) 640
Csp6I GTAC 7 cut(s) 328, 664, 959, 997, 1239, 1389, 2312
CviQI GTAC 7 cut(s) 328, 664, 959, 997, 1239, 1389, 2312
DdeI CTNAG 6 cut(s) 294, 324, 620, 915, 1063, 1929
DraI TTTAAA 1 cut(s) 1743
EaeI YGGCCR 3 cut(s) 65, 108, 2235
EciI GGCGGA 2 cut(s) 352, 672
Ecl136II GAGCTC 1 cut(s) 316
Eco130I CCWWGG 3 cut(s) 75, 1051, 2011
Eco24I GRGCYC 2 cut(s) 318, 883
Eco31I GGTCTC 3 cut(s) 290, 623, 631
Eco47I GGWCC 7 cut(s) 526, 644, 778, 905, 1289, 1316, 2125
Eco53kI GAGCTC 1 cut(s) 316
Eco57I CTGAAG 2 cut(s) 1518, 2282
Eco72I CACGTG 1 cut(s) 414
EcoICRI GAGCTC 1 cut(s) 316
EcoO109I RGGNCCY 2 cut(s) 920, 1316
EcoRI GAATTC 1 cut(s) 690
EcoRII CCWGG 8 cut(s) 307, 640, 812, 868, 922, 1441, 2067, 2172
EcoT14I CCWWGG 3 cut(s) 75, 1051, 2011
EcoT38I GRGCYC 2 cut(s) 318, 883
ErhI CCWWGG 3 cut(s) 75, 1051, 2011
FalI AAGNNNNNCTT 4 cut(s) 793, 825, 1606, 1638
FaqI GGGAC 3 cut(s) 370, 2123, 2138
FauI CCCGC 2 cut(s) 279, 719
FbaI TGATCA 1 cut(s) 1426
FblI GTMKAC 2 cut(s) 216, 865
FokI GGATG 7 cut(s) 61, 131, 776, 998, 1717, 1939, 1958
FriOI GRGCYC 2 cut(s) 318, 883
FspBI CTAG 5 cut(s) 977, 1148, 1422, 1533, 2142
GlaI GCGC 7 cut(s) 165, 210, 212, 260, 453, 489, 928
HaeII RGCGCY 1 cut(s) 455
HaeIII GGCC 9 cut(s) 67, 86, 110, 787, 922, 1056, 1224, 1377, 2237
HapII CCGG 4 cut(s) 26, 130, 389, 2241
HgaI GACGC 2 cut(s) 202, 1797
HhaI GCGC 7 cut(s) 166, 211, 213, 261, 454, 490, 929
Hin6I GCGC 7 cut(s) 164, 209, 211, 259, 452, 488, 927
HinP1I GCGC 7 cut(s) 164, 209, 211, 259, 452, 488, 927
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 1483
HinfI GANTC 7 cut(s) 359, 1181, 1637, 1646, 1985, 2053, 2181
HpaII CCGG 4 cut(s) 26, 130, 389, 2241
HphI GGTGA 3 cut(s) 214, 323, 1718
Hpy166II GTNNAC 5 cut(s) 217, 330, 819, 866, 1241
Hpy188III TCNNGA 7 cut(s) 235, 977, 1859, 1885, 2099, 2123, 2319
Hpy8I GTNNAC 5 cut(s) 217, 330, 819, 866, 1241
Hpy99I CGWCG 3 cut(s) 41, 218, 1791
HpyAV CCTTC 7 cut(s) 23, 256, 667, 1199, 1255, 1493, 2285
HpyCH4III ACNGT 7 cut(s) 996, 1256, 1402, 1450, 1970, 2031, 2129
HpyCH4IV ACGT 3 cut(s) 60, 413, 961
HpyF10VI GCNNNNNNNGC 7 cut(s) 183, 249, 258, 909, 1334, 1930, 1958
HpyF3I CTNAG 6 cut(s) 294, 324, 620, 915, 1063, 1929
HpySE526I ACGT 3 cut(s) 60, 413, 961
HspAI GCGC 7 cut(s) 164, 209, 211, 259, 452, 488, 927
KpnI GGTACC 1 cut(s) 667
Ksp22I TGATCA 1 cut(s) 1426
LmnI GCTCC 5 cut(s) 159, 174, 607, 857, 2098
Lsp1109I GCAGC 7 cut(s) 161, 462, 829, 1585, 1864, 1911, 1973
LweI GCATC 7 cut(s) 126, 976, 1085, 1500, 1645, 1961, 2302
MabI ACCWGGT 1 cut(s) 640
MaeI CTAG 5 cut(s) 977, 1148, 1422, 1533, 2142
MaeII ACGT 3 cut(s) 60, 413, 961
MaeIII GTNAC 6 cut(s) 132, 706, 849, 1519, 1706, 2036
MbiI CCGCTC 1 cut(s) 169
MboII GAAGA 5 cut(s) 391, 1214, 1321, 2248, 2288
MfeI CAATTG 6 cut(s) 465, 611, 648, 1119, 1460, 2111
MflI RGATCY 3 cut(s) 231, 237, 973
MhlI GDGCHC 4 cut(s) 318, 408, 821, 883
MlsI TGGCCA 1 cut(s) 67
MluNI TGGCCA 1 cut(s) 67
MlyI GAGTC 1 cut(s) 1646
MmeI TCCRAC 6 cut(s) 614, 741, 1650, 1810, 1923, 1962
Mox20I TGGCCA 1 cut(s) 67
MroXI GAANNNNTTC 2 cut(s) 1572, 1621
MscI TGGCCA 1 cut(s) 67
MseI TTAA 7 cut(s) 1002, 1742, 1779, 1853, 1901, 1917, 2198
MslI CAYNNNNRTG 3 cut(s) 77, 416, 824
Msp20I TGGCCA 1 cut(s) 67
MspA1I CMGCKG 3 cut(s) 174, 1042, 1964
MspI CCGG 4 cut(s) 26, 130, 389, 2241
MunI CAATTG 6 cut(s) 465, 611, 648, 1119, 1460, 2111
MvaI CCWGG 8 cut(s) 309, 642, 814, 870, 924, 1443, 2069, 2174
MvnI CGCG 5 cut(s) 164, 211, 213, 287, 929
MwoI GCNNNNNNNGC 7 cut(s) 183, 249, 258, 909, 1334, 1930, 1958
NciI CCSGG 2 cut(s) 26, 389
NcoI CCATGG 1 cut(s) 2011
NlaIV GGNNCC 7 cut(s) 514, 665, 921, 993, 1291, 2126, 2170
NmuCI GTSAC 2 cut(s) 132, 1706
NspI RCATGY 1 cut(s) 1849
NspV TTCGAA 2 cut(s) 1259, 1697
OliI CACNNNNGTG 2 cut(s) 77, 824
PagI TCATGA 2 cut(s) 1884, 2098
PauI GCGCGC 1 cut(s) 209
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PdmI GAANNNNTTC 2 cut(s) 1572, 1621
PfeI GAWTC 6 cut(s) 359, 1181, 1646, 1985, 2053, 2181
PflFI GACNNNGTC 1 cut(s) 642
PflMI CCANNNNNTGG 4 cut(s) 234, 535, 653, 2233
PleI GAGTC 1 cut(s) 1645
PmaCI CACGTG 1 cut(s) 414
PmlI CACGTG 1 cut(s) 414
PpsI GAGTC 1 cut(s) 1645
Ppu21I YACGTR 1 cut(s) 414
PpuMI RGGWCCY 1 cut(s) 1316
PshBI ATTAAT 1 cut(s) 1901
Psp124BI GAGCTC 1 cut(s) 318
Psp5II RGGWCCY 1 cut(s) 1316
Psp6I CCWGG 8 cut(s) 307, 640, 812, 868, 922, 1441, 2067, 2172
PspCI CACGTG 1 cut(s) 414
PspGI CCWGG 8 cut(s) 307, 640, 812, 868, 922, 1441, 2067, 2172
PspN4I GGNNCC 7 cut(s) 514, 665, 921, 993, 1291, 2126, 2170
PspPI GGNCC 8 cut(s) 526, 644, 778, 905, 920, 1289, 1316, 2125
PspPPI RGGWCCY 1 cut(s) 1316
PstI CTGCAG 2 cut(s) 847, 1473
PstNI CAGNNNCTG 2 cut(s) 1448, 2305
PsuI RGATCY 3 cut(s) 231, 237, 973
PsyI GACNNNGTC 1 cut(s) 642
PteI GCGCGC 1 cut(s) 209
PvuII CAGCTG 2 cut(s) 1042, 1964
RsaI GTAC 7 cut(s) 329, 665, 960, 998, 1240, 1390, 2313
RsaNI GTAC 7 cut(s) 328, 664, 959, 997, 1239, 1389, 2312
RseI CAYNNNNRTG 3 cut(s) 77, 416, 824
SacI GAGCTC 1 cut(s) 318
SalI GTCGAC 1 cut(s) 215
SaqAI TTAA 7 cut(s) 1002, 1742, 1779, 1853, 1901, 1917, 2198
Sau96I GGNCC 8 cut(s) 526, 644, 778, 905, 920, 1289, 1316, 2125
ScaI AGTACT 1 cut(s) 1390
SchI GAGTC 1 cut(s) 1646
SduI GDGCHC 4 cut(s) 318, 408, 821, 883
SexAI ACCWGGT 1 cut(s) 640
SfaNI GCATC 7 cut(s) 126, 976, 1085, 1500, 1645, 1961, 2302
SfcI CTRYAG 4 cut(s) 843, 1338, 1469, 1602
SfuI TTCGAA 2 cut(s) 1259, 1697
SinI GGWCC 7 cut(s) 526, 644, 778, 905, 1289, 1316, 2125
SmiMI CAYNNNNRTG 3 cut(s) 77, 416, 824
SmlI CTYRAG 1 cut(s) 1244
SmoI CTYRAG 1 cut(s) 1244
SspMI CTAG 5 cut(s) 977, 1148, 1422, 1533, 2142
SstI GAGCTC 1 cut(s) 318
StyI CCWWGG 3 cut(s) 75, 1051, 2011
TaaI ACNGT 7 cut(s) 996, 1256, 1402, 1450, 1970, 2031, 2129
TaiI ACGT 3 cut(s) 63, 416, 964
TatI WGTACW 3 cut(s) 327, 1388, 2311
TauI GCSGC 6 cut(s) 113, 169, 287, 368, 992, 2240
TfiI GAWTC 6 cut(s) 359, 1181, 1646, 1985, 2053, 2181
Tru1I TTAA 7 cut(s) 1002, 1742, 1779, 1853, 1901, 1917, 2198
Tru9I TTAA 7 cut(s) 1002, 1742, 1779, 1853, 1901, 1917, 2198
TscAI CASTG 5 cut(s) 111, 753, 788, 919, 2036
TseFI GTSAC 2 cut(s) 132, 1706
TseI GCWGC 7 cut(s) 174, 475, 842, 1598, 1877, 1924, 1961
Tsp45I GTSAC 2 cut(s) 132, 1706
TspDTI ATGAA 8 cut(s) 546, 780, 1038, 1124, 1842, 1902, 1977, 2244
TspGWI ACGGA 4 cut(s) 54, 791, 1543, 2276
TspRI CASTG 5 cut(s) 111, 753, 788, 919, 2036
Tth111I GACNNNGTC 1 cut(s) 642
Van91I CCANNNNNTGG 4 cut(s) 234, 535, 653, 2233
VneI GTGCAC 1 cut(s) 817
VpaK11BI GGWCC 7 cut(s) 526, 644, 778, 905, 1289, 1316, 2125
VspI ATTAAT 1 cut(s) 1901
XapI RAATTY 3 cut(s) 690, 1272, 1700
XbaI TCTAGA 1 cut(s) 976
XceI RCATGY 1 cut(s) 1849
XmiI GTMKAC 2 cut(s) 216, 865
XmnI GAANNNNTTC 2 cut(s) 1572, 1621
XspI CTAG 5 cut(s) 977, 1148, 1422, 1533, 2142
ZrmI AGTACT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.