FvH4_4g13920

SAC3/GANP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007937
Physical Location & Seq
Forward (+)
17438373 .. 17449834
11462 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13920.t5

Sequence Viewer

Length: 5031 bp
ATGTCGTTTAAAAGCTTCTCGGGCCAGGCGGGACCCGCCGGACCCGCCGGAGAAAAACCCATTCCATTCCAATTCGGCACTGCCGGCCGCCGCCCTCCCTCACCTTCTCCCCCAATCACCCCTCCTTTCCCCAACTCCGCCCCTCTCCGATCTCCTCCCAGACCTTCAGCTGTAGGGCAACCGCATTCGCCACAGTTGGCATTTCAAGGTTCTCATCCGGGTGCAACTCATTCTTACCGACCTTCTGGAGTGCAAAGGTCCCCTGAATCTCTTCCTAGTTGGAATGGTGCGCAGAGACCCTTGTTGAAAAATAATGATACCATGGTTCATCAAAGACCATCTGCCGTCACATCATTTGTTGTTTCTCGTAATTCTGGAAATAGTGTTACTGCCAAGATTTCCAGATTCCAAGACACGCGAGGGAACAGATCTCCTCCCTTTGTGTCAAGAGATGTAAATATAAGAAATTCCACTCAAAGTGTTCCGAGAAGTCATGTAGTTCCTCCAAGAACGCAATCACCTACCTTGACTTCATATAACTATCAGCCTGTTGAGGATTTCAATCATGTTGGTGGAGATGAAGGTCACTTGTTTCCTCCAAGAACACGGTCACCGCCCTCATCATCTTACAACTATCATCCTGTTGAGGATTTTGATCGTTTTGGTGGAGTTGACGGTAACTTGGTTACTCCACAAACACGATCACCTCCCTCAGGCTCCTATAACCATCGTCCTGTTGAGGACTTTGATCGTTCTGGTGGAGTTTTAGGGCATGTATTTTCCTCTTCTGAACTGAATAGTTCGCCAAAGTCGATAGGTGATCATGCCAGATTGCAGGCCTATCAAGAACCATCTCGAGTATCACCTTCTGTCAGCTCTGTTGACTCTGGTAGAAGAACTCCCGTCAACTATGATGACGTCCAAGTCCTGAAACGAACTAGGCCTTCTGTATCTCCTGTGGGGTCAAATGCTACTTCAAGTGCAATTTTCAATACTCGCGATTCTCGGGTTCAGCAAAAATCTTTGCAGTCTTCAAACAATACCCTTTCTGAAGCTGTTGCAAACAATTTGACCGACATACCCATTGCCAAAAGAATGAGGTCGCCTCCTTTACTTCCCGAGGATCAAATCTTCAAGGGAGACTCATATGCCACTCAAGACGGTACTGAACGAGAAATGCAAGCAAAAGCAAAGAGATTAGCCCGCTTCAAGGTTGAATTAAGCAAAAGTCCACACAATGGTAATGATATCGTAGAGCAGGGCGTTTCTGCGTCTAGAAATGAACAATCTAATGTTGAGAGGAACAGATCAGTTGCATATAGTTCTACTCAACTGGCTAGGGATGTTACTGATGGAAATGCTGTATCCGAGTGTGAAGGCGTAGAATCCTCTGGTATCATTATTGGTGTATGCCCAGATATGTGTCCAGATTCAGAGAGAGCGGAGCGAGAAAGGAAAGGGGATCTTGATCAGCATGAGCGTGTGGATGGGGATAGAAATCAAACTAGCATGTCCCTTGCTGTTAAAAAGTATAACAGGACAGCTGAGCGGGATGCAAACTTGATAAGACCAATGCCGATCCTGCAGAATACAATGGATTATTTGCTCAGTCTGCTTGATAAACCTTACAACGATACGTTTCTTAGCATATATAACTTCTTGTGGGATAGAATGCGGGCAATTCGAATGGACCTAAGAATGCAACACATCTTTGACCAAGAGGCTATTAATATGTTGGAGCAAATGATAAGACTTCACATAATTGCCATGCACGAGTTATGTGAATACTCAAGAGGAGAGGGGTTTGCTGAAGGATTTGATGCTCACCTCAATATTGAGCAGATGAATAAAACATCAGTAGAATTGTTCCAATTGTACGATGATCACAGAAAGCAGGGAATAAATATTCCTACAGAAAAAGAGTTTCGAGGTTACTATGCGCTTCTCAAGCTGGACAAACATCCTGGGCATATGGTTGAACCTGCTGAGCTCTCACTTGATCTTGCAAAGATGACCCCAGAAATAAGACAAACTTCAGAGGTGCTATTGGCACGTGATGTAGCAAGAGCTTGTAGAACTGGTAATTTCATTGCCTTCTTTCGACTTGCAAGAAAAGCAACTTATCTTCAAGCATGCCTGATGCACGCTCATTTTGCAAAGTTACGAACGCTGGCACTTGCTTCTCTACAAGCAGGTCTCCAGAATAACCAAGGTCTCCCTATTGCGGATGTTGCCAAGTGGCTAGCTATGGAGGAAGAGGAAATTGAAAGCCTTTCAGTGTATCATGGATTCCAGTTGAAATCTTACAATAAGGAGCCATATATTGTAAAGGAAGGTCCATTTCTAAATGGTGATGAAGAATATCCTACAAAGTGTTCTAAACTTGTGGACATGAAAAAATCAAGAAGGATAATGAAGGATGTTATAGCTTCTGGTCAAGTTGTATCCTTGCCTGCAGAAGCTTCAAATGAAACTCAGCTGACTAAGCCAAATACACTTGGAGCAAAAAGTTCGTCATATGGTGAAGGGGGAAGTCTTATCCAGAATGTACTATCTGTTCCTGTAGTAAATTCCATCCCTGAACTTGATGAAGAAATGCCCAATTGTGAAGTTGTTTCATCCCCAAGAGATATCAGTCCAAGGCAGATAAGAATTCCAACATCTATTTTCTCACCCCAAACAGATGTCAGACAAAAGCAGCATATGATTCAAACGCCACTAGCGTTGTCCCCAAAGGATAGCAGAGAGCAGCAAGTGATTAACATGCCATTTGTTGGTCGTCGTCATGATGATAATCCGATGGTTTCTTTGTCTCCTTCCCCTTGGGATCTCTCATCATTTAAACCCCAACCTGATAAAGTTGGTTTAAATGAGAAGGCCAACAGAGATGCTTTTTACTGTAACTTTCCAGAGAAAAGCATGCATTTTGGTATGGAAGCAATGCCATTGCAGATTGTGTCAAAAACATCTCTGCAATCTGCAGTTGGTACAAATAGAGATGAGGCGGAACATTCAGCAGGCCAAATTGTGTCTAATAACTTGGACAATGACGAACCTACAGATCTTCCTCAGGACAATGAAAGTGACGAGGATATGGGAAATTATCAACAGGAGGAAATTGCCGAGGCAAAGCTGAAGTTGTTGTTTAGGTTATGGAGGCGGCGTTCTGTGAAGTTAAGGGAACTGCGAGAGCAAAGGCAGTTGATCACAAATGCTGCATTAAATTCCTTATCACTGGGACCACCAATTCAACTGAAGAGAGATCAACCCCACATGCCTGGTGGCTTTGACATTGATCGAATTTTGAGGGAGAGACATCAAAAGCAAGGCCTGTCCCAGTCCAGTCTAAATGTTTCAGATGTAATAGCAGATACACTAAGCACAAGAAACCCGGATGCTAGATGCCTGTGTTGGAAAATTGTTGTATATTCTCAGATGAACAATATGGAAGGTGATGAACTCTGGCAGAGGAACCATGCCCTCGAAGCAGCACCCTGGTTGCTTTCAAAGCTCATGCCTTCAAAAAACGATGATGAGGATCTCTTAATTTCATCTCCTGGCACCTCAATATGGAAGAAATGGTTTGAAGGCGAATCTGGTTCAGACCTGACCTGCTGTTTATCTGTAGTTAAGGATGCAAACAGTGATAATCTAAATGAGTGTGTATCTGGTATAAGTGCACTTTTGTTTCTTGTGTCAGAAAGTATCCCTTGGAAATTGCAAAAAGTCCAGCTCAATAACCTTTTGATGTCAGTACCGTATGGCTCCTGCTTACCTCTTCTGATCCTAGCTGGTTCGTTCAAGAATGTTGCCGATCCTTCCTCCATTATTGTTAGTAATATGGGTCTCCATGACTTGGATAAATCCCGAATACGCAGCTTTCGGATTGTTTCCCTTCTTGAAAATCAGAAAAGAGAGCAATTGGACGGGTTCTATAGTGACAACCGACTAAGGGAGGGACTCAGATGGCTTGCAAGTGAATCACCTCCTCAACCCATTCTTCATCATGTGAAAACACATGAATTGATTCTGACGCACCTGAATTCTTCATTGAAGGCACTAGAGAAATTGAAAGATTATGAGGTAGGTCCGAACGACTGTATCCTAGCCTTCAATGAAGCTTTGGATCAGTCCCAAAGGGAAATTGCTGCTGCTGTTCAAGCAAATCCTGCTGGTTTGCCTTGCCCTGAGATTGCTTTACTGGAGGGATTCGATGAAGAACACAGGCTAGTGAAGTGGTGCTTGCCGAGGATAGGATGGAGCTCAGTGGCAAAAATAGAATCACTAATTTCAGCTCTTGGGAACTGTAGACTTCCCACTTTTCCTAATAGTATCTCATGGTTGCCGAGATGTTCGAATGCAAGGAAGGAGATTGAAAGCCTGAGAGTTGAACTTGAAAATGGCTTAATCGGATACCTTGCAGATTCTAAGACATTGGGACCTGCACTTGCTATAAAAGAGGCACATGTAATGCTACAAAGAAGTTGTCGGCTTCAGTGCCAGGACTCATGCTGCTACATTGTGCCCAAGTGGACTATGATTTTTCGACGAATCTTTAATTGGCGGCTGATGGGTTTGGCTAACGGGACATTCGCTTCAGCTTATATCCTGGAATGCCCTCATCTCAATGCAACTTTCGGAAACCTGGGTAAGTTGGAACTTGAAGATAGAGAACCTTCAGCATATCACTTCAATCAGCTGACTCTGGATGAGGTCATTGAAGTTTGCCGCAGCCCTCTAATGTTTCAGAGAGATCAGTCGTTGCAAGAAACTGATGGAACTATTCCGGAGACATCTCCAAATGGTTCAGTTCATGAGACTCCGAGTACATATGATCTTATGGATGATGAGACATGCTTAACCGATGATATAGAGGACGTGTCTCACGTAAATAGGGGACTAGAAAGTGGTGGCAGAGAAATGATGGTGGCTGGGAAGGAAACCAAGGAAGATGATAGATTGAATATATTGTTGAAGCACTGTAACATGTTACAAAATGGCATAGATAAGAAGTTGTCCATTTATTTCTAA

Protein Analysis

1677

Amino Acids

187.44

Weight (kDa)

6.08

Isoelectric Point (pI)

57.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 417 - 710 3.6e-80 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 923
AatII GACGTC 1 cut(s) 921
Acc16I TGCGCA 1 cut(s) 291
Acc36I ACCTGC 4 cut(s) 1990, 2183, 3620, 4450
AccB1I GGYRCC 1 cut(s) 3560
AccB7I CCANNNNNTGG 3 cut(s) 1238, 2774, 3856
AccBSI CCGCTC 2 cut(s) 1442, 1549
AccI GTMKAC 1 cut(s) 4309
AccII CGCG 2 cut(s) 418, 999
AccIII TCCGGA 1 cut(s) 4786
AclWI GGATC 8 cut(s) 1131, 1470, 1573, 2835, 3546, 3778, 3809, 4134
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 6 cut(s) 466, 2569, 2652, 3223, 3300, 4042
AcuI CTGAAG 9 cut(s) 150, 1071, 1830, 2019, 3155, 3275, 4478, 4581, 4662
AcvI CACGTG 1 cut(s) 2054
AcyI GRCGYC 1 cut(s) 918
AfaI GTAC 6 cut(s) 1165, 1877, 2550, 2989, 3756, 4828
AfiI CCNNNNNNNGG 9 cut(s) 713, 1210, 1238, 2224, 2774, 3570, 3856, 3952, 4253
AflIII ACRYGT 3 cut(s) 4465, 4878, 4986
AjiI CACGTC 1 cut(s) 4879
AjnI CCWGG 8 cut(s) 24, 1963, 3277, 3494, 3556, 4500, 4608, 4644
AjuI GAANNNNNNNTTGG 2 cut(s) 4543, 4575
AloI GAACNNNNNNTCC 2 cut(s) 3148, 3180
Alw21I GWGCWC 3 cut(s) 1992, 3682, 4265
Alw44I GTGCAC 1 cut(s) 3678
AlwI GGATC 8 cut(s) 1131, 1470, 1573, 2835, 3546, 3778, 3809, 4134
Ama87I CYCGRG 4 cut(s) 19, 855, 1005, 1118
Aor13HI TCCGGA 1 cut(s) 4786
AoxI GGCC 7 cut(s) 22, 85, 837, 941, 2877, 3019, 3328
ApaLI GTGCAC 1 cut(s) 3678
ApeKI GCWGC 9 cut(s) 2698, 2749, 3215, 3488, 3876, 4148, 4151, 4512, 4731
ApoI RAATTY 6 cut(s) 466, 2569, 2652, 3223, 3300, 4042
ArsI GACNNNNNNTTYG 4 cut(s) 2498, 2530, 4471, 4503
AseI ATTAAT 1 cut(s) 1728
Asp700I GAANNNNTTC 2 cut(s) 270, 4026
AspLEI GCGC 2 cut(s) 292, 1942
AspS9I GGNCC 9 cut(s) 22, 32, 41, 258, 1690, 2336, 3239, 4088, 4439
AsuC2I CCSGG 2 cut(s) 219, 3392
AsuII TTCGAA 2 cut(s) 1684, 4355
AsuNHI GCTAGC 1 cut(s) 2242
AvaI CYCGRG 4 cut(s) 19, 855, 1005, 1118
AvaII GGWCC 8 cut(s) 32, 41, 258, 1690, 2336, 3239, 4088, 4439
AxyI CCTNAGG 2 cut(s) 712, 3069
BaeGI GKGCMC 2 cut(s) 3682, 4527
BaeI ACNNNNGTAYC 4 cut(s) 309, 342, 4084, 4117
BanI GGYRCC 1 cut(s) 3560
BanII GRGCYC 2 cut(s) 1992, 4265
BauI CACGAG 1 cut(s) 1772
BbrPI CACGTG 1 cut(s) 2054
BbsI GAAGAC 1 cut(s) 1023
Bbv12I GWGCWC 3 cut(s) 1992, 3682, 4265
BbvI GCAGC 9 cut(s) 2710, 2761, 3202, 3500, 3888, 4135, 4138, 4499, 4743
BceAI ACGGC 1 cut(s) 329
BcgI CGANNNNNNTGC 2 cut(s) 2493, 2527
BciT130I CCWGG 8 cut(s) 26, 1965, 3279, 3496, 3558, 4502, 4610, 4646
BciVI GTATCC 5 cut(s) 1375, 2455, 3716, 4112, 4406
BclI TGATCA 4 cut(s) 820, 1468, 1882, 3204
BcnI CCSGG 2 cut(s) 219, 3392
BfuAI ACCTGC 4 cut(s) 1990, 2183, 3620, 4450
BfuI GTATCC 5 cut(s) 1375, 2455, 3716, 4112, 4406
BglII AGATCT 2 cut(s) 428, 3061
BlpI GCTNAGC 2 cut(s) 1545, 1986
Bme18I GGWCC 8 cut(s) 32, 41, 258, 1690, 2336, 3239, 4088, 4439
BmeT110I CYCGRG 4 cut(s) 19, 855, 1005, 1118
BmgBI CACGTC 1 cut(s) 4879
BmgT120I GGNCC 9 cut(s) 22, 32, 41, 258, 1690, 2336, 3239, 4088, 4439
BmrI ACTGGG 2 cut(s) 3245, 3331
BmsI GCATC 7 cut(s) 1543, 1810, 2130, 2878, 3385, 3392, 3625
BmtI GCTAGC 1 cut(s) 2246
BmuI ACTGGG 2 cut(s) 3245, 3331
BpiI GAAGAC 1 cut(s) 1023
BplI GAGNNNNNCTC 2 cut(s) 1090, 1122
BpmI CTGGAG 3 cut(s) 267, 2183, 4223
Bpu1102I GCTNAGC 2 cut(s) 1545, 1986
Bpu14I TTCGAA 2 cut(s) 1684, 4355
BpuEI CTTGAG 3 cut(s) 1140, 1774, 1931
BpuMI CCSGG 2 cut(s) 219, 3392
BsaAI YACGTR 2 cut(s) 2054, 4888
BsaBI GATNNNNATC 6 cut(s) 561, 654, 1467, 1497, 2793, 3537
BsaHI GRCGYC 1 cut(s) 918
BsaI GGTCTC 4 cut(s) 289, 2201, 2219, 3851
BsaWI WCCGGW 1 cut(s) 4786
BsaXI ACNNNNNCTCC 2 cut(s) 3148, 3178
Bsc4I CCNNNNNNNGG 9 cut(s) 713, 1210, 1238, 2224, 2774, 3570, 3856, 3952, 4253
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 7 cut(s) 1338, 2083, 2293, 3240, 3337, 3342, 4206
Bse21I CCTNAGG 2 cut(s) 712, 3069
Bse3DI GCAATG 4 cut(s) 1083, 2088, 2945, 2946
Bse8I GATNNNNATC 6 cut(s) 561, 654, 1467, 1497, 2793, 3537
BseAI TCCGGA 1 cut(s) 4786
BseBI CCWGG 8 cut(s) 26, 1965, 3279, 3496, 3558, 4502, 4610, 4646
BseJI GATNNNNATC 6 cut(s) 561, 654, 1467, 1497, 2793, 3537
BseLI CCNNNNNNNGG 9 cut(s) 713, 1210, 1238, 2224, 2774, 3570, 3856, 3952, 4253
BseMI GCAATG 4 cut(s) 1083, 2088, 2945, 2946
BseNI ACTGG 7 cut(s) 1338, 2083, 2293, 3240, 3337, 3342, 4206
BseRI GAGGAG 4 cut(s) 144, 423, 1809, 3978
BseSI GKGCMC 2 cut(s) 3682, 4527
BseX3I CGGCCG 1 cut(s) 85
BseXI GCAGC 9 cut(s) 2710, 2761, 3202, 3500, 3888, 4135, 4138, 4499, 4743
BseYI CCCAGC 1 cut(s) 4931
BsgI GTGCAG 1 cut(s) 4428
Bsh1236I CGCG 2 cut(s) 418, 999
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 7 cut(s) 24, 87, 839, 943, 2879, 3021, 3330
BshNI GGYRCC 1 cut(s) 3560
BsiEI CGRYCG 1 cut(s) 88
BsiHKAI GWGCWC 3 cut(s) 1992, 3682, 4265
BsiHKCI CYCGRG 4 cut(s) 19, 855, 1005, 1118
BsiSI CCGG 6 cut(s) 39, 48, 84, 218, 3392, 4787
BslI CCNNNNNNNGG 9 cut(s) 713, 1210, 1238, 2224, 2774, 3570, 3856, 3952, 4253
BsmI GAATGC 5 cut(s) 184, 1677, 1704, 4363, 4619
BsnI GGCC 7 cut(s) 24, 87, 839, 943, 2879, 3021, 3330
Bso31I GGTCTC 4 cut(s) 289, 2201, 2219, 3851
BsoBI CYCGRG 4 cut(s) 19, 855, 1005, 1118
Bsp119I TTCGAA 2 cut(s) 1684, 4355
Bsp1286I GDGCHC 4 cut(s) 1992, 3682, 4265, 4527
Bsp13I TCCGGA 1 cut(s) 4786
Bsp1720I GCTNAGC 2 cut(s) 1545, 1986
Bsp19I CCATGG 1 cut(s) 321
Bsp68I TCGCGA 1 cut(s) 999
BspANI GGCC 7 cut(s) 24, 87, 839, 943, 2879, 3021, 3330
BspEI TCCGGA 1 cut(s) 4786
BspFNI CGCG 2 cut(s) 418, 999
BspHI TCATGA 2 cut(s) 2785, 4813
BspMAI CTGCAG 3 cut(s) 1587, 2458, 2983
BspMI ACCTGC 4 cut(s) 1990, 2183, 3620, 4450
BspOI GCTAGC 1 cut(s) 2246
BspPI GGATC 8 cut(s) 1131, 1470, 1573, 2835, 3546, 3778, 3809, 4134
BspT104I TTCGAA 2 cut(s) 1684, 4355
BspT107I GGYRCC 1 cut(s) 3560
BspTNI GGTCTC 4 cut(s) 289, 2201, 2219, 3851
BsrBI CCGCTC 2 cut(s) 1442, 1549
BsrDI GCAATG 4 cut(s) 1083, 2088, 2945, 2946
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 7 cut(s) 1338, 2083, 2293, 3240, 3337, 3342, 4206
BssAI RCCGGY 1 cut(s) 83
BssNI GRCGYC 1 cut(s) 918
BssSI CACGAG 1 cut(s) 1772
BssT1I CCWWGG 6 cut(s) 321, 2209, 2639, 2822, 3710, 4944
Bst2BI CACGAG 1 cut(s) 1772
Bst2UI CCWGG 8 cut(s) 26, 1965, 3279, 3496, 3558, 4502, 4610, 4646
Bst6I CTCTTC 5 cut(s) 276, 790, 2250, 3251, 3783
BstACI GRCGYC 1 cut(s) 918
BstAPI GCANNNNNTGC 1 cut(s) 4169
BstBAI YACGTR 2 cut(s) 2054, 4888
BstBI TTCGAA 2 cut(s) 1684, 4355
BstDSI CCRYGG 1 cut(s) 321
BstEII GGTNACC 1 cut(s) 609
BstENI CCTNNNNNAGG 1 cut(s) 711
BstFNI CGCG 2 cut(s) 418, 999
BstHHI GCGC 2 cut(s) 292, 1942
BstMCI CGRYCG 1 cut(s) 88
BstNI CCWGG 8 cut(s) 26, 1965, 3279, 3496, 3558, 4502, 4610, 4646
BstNSI RCATGY 9 cut(s) 776, 1513, 2136, 2767, 2923, 3277, 4469, 4857, 4990
BstPI GGTNACC 1 cut(s) 609
BstSLI GKGCMC 2 cut(s) 3682, 4527
BstUI CGCG 2 cut(s) 418, 999
BstV1I GCAGC 9 cut(s) 2710, 2761, 3202, 3500, 3888, 4135, 4138, 4499, 4743
BstV2I GAAGAC 1 cut(s) 1023
BstX2I RGATCY 5 cut(s) 428, 1462, 2827, 3061, 3538
BstXI CCANNNNNNTGG 1 cut(s) 3278
BstYI RGATCY 5 cut(s) 428, 1462, 2827, 3061, 3538
BstZI CGGCCG 1 cut(s) 85
Bsu36I CCTNAGG 2 cut(s) 712, 3069
BsuI GTATCC 5 cut(s) 1375, 2455, 3716, 4112, 4406
BsuRI GGCC 7 cut(s) 24, 87, 839, 943, 2879, 3021, 3330
BtgI CCRYGG 1 cut(s) 321
BtrI CACGTC 1 cut(s) 4879
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 7 cut(s) 78, 2283, 3233, 3649, 4272, 4502, 4978
BtuMI TCGCGA 1 cut(s) 999
BveI ACCTGC 4 cut(s) 1990, 2183, 3620, 4450
CciI TCATGA 2 cut(s) 2785, 4813
CfoI GCGC 2 cut(s) 292, 1942
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 9 cut(s) 22, 32, 41, 258, 1690, 2336, 3239, 4088, 4439
CseI GACGC 2 cut(s) 1260, 4042
Csp6I GTAC 6 cut(s) 1164, 1876, 2549, 2988, 3755, 4827
CspCI CAANNNNNGTGG 2 cut(s) 2706, 2741
CviQI GTAC 6 cut(s) 1164, 1876, 2549, 2988, 3755, 4827
DraI TTTAAA 3 cut(s) 10, 2842, 2868
DrdI GACNNNNNNGTC 1 cut(s) 923
DseDI GACNNNNNNGTC 1 cut(s) 923
EaeI YGGCCR 1 cut(s) 85
EagI CGGCCG 1 cut(s) 85
Eam1104I CTCTTC 5 cut(s) 276, 790, 2250, 3251, 3783
EarI CTCTTC 5 cut(s) 276, 790, 2250, 3251, 3783
EciI GGCGGA 2 cut(s) 127, 3020
Ecl136II GAGCTC 2 cut(s) 1990, 4263
EclXI CGGCCG 1 cut(s) 85
Eco130I CCWWGG 6 cut(s) 321, 2209, 2639, 2822, 3710, 4944
Eco147I AGGCCT 3 cut(s) 839, 943, 3330
Eco24I GRGCYC 2 cut(s) 1992, 4265
Eco31I GGTCTC 4 cut(s) 289, 2201, 2219, 3851
Eco32I GATATC 2 cut(s) 1249, 2632
Eco47I GGWCC 8 cut(s) 32, 41, 258, 1690, 2336, 3239, 4088, 4439
Eco52I CGGCCG 1 cut(s) 85
Eco53kI GAGCTC 2 cut(s) 1990, 4263
Eco57I CTGAAG 9 cut(s) 150, 1071, 1830, 2019, 3155, 3275, 4478, 4581, 4662
Eco72I CACGTG 1 cut(s) 2054
Eco81I CCTNAGG 2 cut(s) 712, 3069
Eco88I CYCGRG 4 cut(s) 19, 855, 1005, 1118
Eco91I GGTNACC 1 cut(s) 609
EcoICRI GAGCTC 2 cut(s) 1990, 4263
EcoNI CCTNNNNNAGG 1 cut(s) 711
EcoO109I RGGNCCY 3 cut(s) 32, 258, 4439
EcoO65I GGTNACC 1 cut(s) 609
EcoRI GAATTC 2 cut(s) 2652, 4042
EcoRII CCWGG 8 cut(s) 24, 1963, 3277, 3494, 3556, 4500, 4608, 4644
EcoRV GATATC 2 cut(s) 1249, 2632
EcoT14I CCWWGG 6 cut(s) 321, 2209, 2639, 2822, 3710, 4944
EcoT22I ATGCAT 1 cut(s) 2925
EcoT38I GRGCYC 2 cut(s) 1992, 4265
ErhI CCWWGG 6 cut(s) 321, 2209, 2639, 2822, 3710, 4944
FalI AAGNNNNNCTT 8 cut(s) 1449, 1481, 2017, 2049, 3694, 3726, 4226, 4258
FauI CCCGC 6 cut(s) 22, 43, 52, 1211, 1542, 1668
FauNDI CATATG 5 cut(s) 1147, 1971, 2518, 2703, 4831
FbaI TGATCA 4 cut(s) 820, 1468, 1882, 3204
FblI GTMKAC 1 cut(s) 4309
FriOI GRGCYC 2 cut(s) 1992, 4265
FspI TGCGCA 1 cut(s) 291
GlaI GCGC 2 cut(s) 291, 1941
GsaI CCCAGC 1 cut(s) 4935
GsuI CTGGAG 3 cut(s) 267, 2183, 4223
HaeIII GGCC 7 cut(s) 24, 87, 839, 943, 2879, 3021, 3330
HapII CCGG 6 cut(s) 39, 48, 84, 218, 3392, 4787
HgaI GACGC 2 cut(s) 1260, 4042
HhaI GCGC 2 cut(s) 292, 1942
Hin1I GRCGYC 1 cut(s) 918
Hin6I GCGC 2 cut(s) 290, 1940
HinP1I GCGC 2 cut(s) 290, 1940
HincII GTYRAC 3 cut(s) 673, 883, 907
HindII GTYRAC 3 cut(s) 673, 883, 907
HindIII AAGCTT 3 cut(s) 13, 2460, 4119
HpaII CCGG 6 cut(s) 39, 48, 84, 218, 3392, 4787
Hpy166II GTNNAC 8 cut(s) 673, 883, 907, 1232, 2389, 3680, 4310, 4533
Hpy8I GTNNAC 8 cut(s) 673, 883, 907, 1232, 2389, 3680, 4310, 4533
Hpy99I CGWCG 2 cut(s) 2784, 4551
HpyCH4IV ACGT 5 cut(s) 918, 1637, 2053, 4878, 4887
HpySE526I ACGT 5 cut(s) 918, 1637, 2053, 4878, 4887
Hsp92I GRCGYC 1 cut(s) 918
HspAI GCGC 2 cut(s) 290, 1940
KflI GGGWCCC 1 cut(s) 32
Kpn2I TCCGGA 1 cut(s) 4786
KroI GCCGGC 1 cut(s) 83
KroNI GCCGGC 1 cut(s) 85
Ksp22I TGATCA 4 cut(s) 820, 1468, 1882, 3204
LmnI GCTCC 7 cut(s) 722, 1444, 1738, 2314, 2501, 3770, 4260
Lsp1109I GCAGC 9 cut(s) 2710, 2761, 3202, 3500, 3888, 4135, 4138, 4499, 4743
LweI GCATC 7 cut(s) 1543, 1810, 2130, 2878, 3385, 3392, 3625
MaeII ACGT 5 cut(s) 918, 1637, 2053, 4878, 4887
MbiI CCGCTC 2 cut(s) 1442, 1549
MfeI CAATTG 3 cut(s) 1871, 2602, 3920
MflI RGATCY 5 cut(s) 428, 1462, 2827, 3061, 3538
MhlI GDGCHC 4 cut(s) 1992, 3682, 4265, 4527
MlyI GAGTC 6 cut(s) 878, 1136, 3954, 4499, 4696, 4813
MmeI TCCRAC 5 cut(s) 260, 1716, 2681, 3392, 4635
Mph1103I ATGCAT 1 cut(s) 2925
MroI TCCGGA 1 cut(s) 4786
MroNI GCCGGC 1 cut(s) 83
MroXI GAANNNNTTC 2 cut(s) 270, 4026
MslI CAYNNNNRTG 4 cut(s) 219, 570, 1479, 4626
MspA1I CMGCKG 4 cut(s) 170, 1544, 2479, 4699
MspI CCGG 6 cut(s) 39, 48, 84, 218, 3392, 4787
MunI CAATTG 3 cut(s) 1871, 2602, 3920
Mva1269I GAATGC 5 cut(s) 184, 1677, 1704, 4363, 4619
MvaI CCWGG 8 cut(s) 26, 1965, 3279, 3496, 3558, 4502, 4610, 4646
MvnI CGCG 2 cut(s) 418, 999
NaeI GCCGGC 1 cut(s) 85
NciI CCSGG 2 cut(s) 219, 3392
NcoI CCATGG 1 cut(s) 321
NdeI CATATG 5 cut(s) 1147, 1971, 2518, 2703, 4831
NgoMIV GCCGGC 1 cut(s) 83
NheI GCTAGC 1 cut(s) 2242
NmeAIII GCCGAG 3 cut(s) 3148, 4272, 4371
NmuCI GTSAC 5 cut(s) 346, 584, 609, 3083, 3938
NruI TCGCGA 1 cut(s) 999
NsbI TGCGCA 1 cut(s) 291
NsiI ATGCAT 1 cut(s) 2925
NspI RCATGY 9 cut(s) 776, 1513, 2136, 2767, 2923, 3277, 4469, 4857, 4990
NspV TTCGAA 2 cut(s) 1684, 4355
PaeI GCATGC 2 cut(s) 2136, 2923
PaeR7I CTCGAG 1 cut(s) 855
PagI TCATGA 2 cut(s) 2785, 4813
PceI AGGCCT 3 cut(s) 839, 943, 3330
PciI ACATGT 2 cut(s) 4465, 4986
PcsI WCGNNNNNNNCGW 3 cut(s) 809, 2720, 4884
PctI GAATGC 5 cut(s) 184, 1677, 1704, 4363, 4619
PdiI GCCGGC 1 cut(s) 85
PdmI GAANNNNTTC 2 cut(s) 270, 4026
PflMI CCANNNNNTGG 3 cut(s) 1238, 2774, 3856
PfoI TCCNGGA 1 cut(s) 4608
PleI GAGTC 6 cut(s) 878, 1136, 3954, 4499, 4696, 4813
PmaCI CACGTG 1 cut(s) 2054
PmlI CACGTG 1 cut(s) 2054
PpsI GAGTC 6 cut(s) 878, 1136, 3954, 4499, 4696, 4813
Ppu21I YACGTR 2 cut(s) 2054, 4888
PpuMI RGGWCCY 3 cut(s) 32, 258, 4439
PscI ACATGT 2 cut(s) 4465, 4986
PshBI ATTAAT 1 cut(s) 1728
Psp124BI GAGCTC 2 cut(s) 1992, 4265
Psp5II RGGWCCY 3 cut(s) 32, 258, 4439
Psp6I CCWGG 8 cut(s) 24, 1963, 3277, 3494, 3556, 4500, 4608, 4644
PspCI CACGTG 1 cut(s) 2054
PspEI GGTNACC 1 cut(s) 609
PspFI CCCAGC 1 cut(s) 4931
PspGI CCWGG 8 cut(s) 24, 1963, 3277, 3494, 3556, 4500, 4608, 4644
PspPI GGNCC 9 cut(s) 22, 32, 41, 258, 1690, 2336, 3239, 4088, 4439
PspPPI RGGWCCY 3 cut(s) 32, 258, 4439
PsrI GAACNNNNNNTAC 2 cut(s) 2541, 2573
PstI CTGCAG 3 cut(s) 1587, 2458, 2983
PsuI RGATCY 5 cut(s) 428, 1462, 2827, 3061, 3538
PvuII CAGCTG 4 cut(s) 170, 1544, 2479, 4699
RruI TCGCGA 1 cut(s) 999
RsaI GTAC 6 cut(s) 1165, 1877, 2550, 2989, 3756, 4828
RsaNI GTAC 6 cut(s) 1164, 1876, 2549, 2988, 3755, 4827
RseI CAYNNNNRTG 4 cut(s) 219, 570, 1479, 4626
SacI GAGCTC 2 cut(s) 1992, 4265
Sau96I GGNCC 9 cut(s) 22, 32, 41, 258, 1690, 2336, 3239, 4088, 4439
SchI GAGTC 6 cut(s) 878, 1136, 3954, 4499, 4696, 4813
SduI GDGCHC 4 cut(s) 1992, 3682, 4265, 4527
SfaNI GCATC 7 cut(s) 1543, 1810, 2130, 2878, 3385, 3392, 3625
Sfr274I CTCGAG 1 cut(s) 855
SfuI TTCGAA 2 cut(s) 1684, 4355
SinI GGWCC 8 cut(s) 32, 41, 258, 1690, 2336, 3239, 4088, 4439
SlaI CTCGAG 1 cut(s) 855
SmiMI CAYNNNNRTG 4 cut(s) 219, 570, 1479, 4626
SmlI CTYRAG 4 cut(s) 855, 1155, 1789, 1946
SmoI CTYRAG 4 cut(s) 855, 1155, 1789, 1946
SphI GCATGC 2 cut(s) 2136, 2923
SseBI AGGCCT 3 cut(s) 839, 943, 3330
SspI AATATT 2 cut(s) 1834, 1906
SstI GAGCTC 2 cut(s) 1992, 4265
StuI AGGCCT 3 cut(s) 839, 943, 3330
StyI CCWWGG 6 cut(s) 321, 2209, 2639, 2822, 3710, 4944
TaiI ACGT 5 cut(s) 921, 1640, 2056, 4881, 4890
TaqII GACCGA 1 cut(s) 1088
TatI WGTACW 2 cut(s) 2548, 4826
TauI GCSGC 5 cut(s) 90, 93, 3163, 4567, 4731
TscAI CASTG 7 cut(s) 85, 2283, 3240, 3649, 4272, 4502, 4985
TseFI GTSAC 5 cut(s) 346, 584, 609, 3083, 3938
TseI GCWGC 9 cut(s) 2698, 2749, 3215, 3488, 3876, 4148, 4151, 4512, 4731
Tsp45I GTSAC 5 cut(s) 346, 584, 609, 3083, 3938
TspRI CASTG 7 cut(s) 85, 2283, 3240, 3649, 4272, 4502, 4985
Van91I CCANNNNNTGG 3 cut(s) 1238, 2774, 3856
VneI GTGCAC 1 cut(s) 3678
VpaK11BI GGWCC 8 cut(s) 32, 41, 258, 1690, 2336, 3239, 4088, 4439
VspI ATTAAT 1 cut(s) 1728
XagI CCTNNNNNAGG 1 cut(s) 711
XapI RAATTY 6 cut(s) 466, 2569, 2652, 3223, 3300, 4042
XbaI TCTAGA 1 cut(s) 1274
XceI RCATGY 9 cut(s) 776, 1513, 2136, 2767, 2923, 3277, 4469, 4857, 4990
XcmI CCANNNNNNNNNTGG 1 cut(s) 3278
XhoI CTCGAG 1 cut(s) 855
XmiI GTMKAC 1 cut(s) 4309
XmnI GAANNNNTTC 2 cut(s) 270, 4026
ZraI GACGTC 1 cut(s) 919
Zsp2I ATGCAT 1 cut(s) 2925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.