Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0007937

Physical Location & Seq

Genomic Coordinates Forward (+)

14879840 .. 14885499

Gene Structure 5660 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1178600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3162 bp

ATGCATGAATTATGTGAATACTCAAGAGGAGAAGGCTTTGCTGAGGGATTTGATGCACACCTCAATATTGAACAGATGAATAAAACATCTGTTGAATTGTTCCAATTGTATGATGATCACAGAAAGAAAGGAATAAACATTCCTACAGAAAAAGAGTTTCGAGGTTATTATGCCCTTCTCAAGCTGGACAAGCATCCTGGGTACATGGTTGAACCTGCAGAGCTCTCGCTTGATCTTGCAAAGATGACTCCAGAAATAAGACAAACTTCAGAGGTGCTATTTGCACGTGATGTCGCTAGAGCTTGCAGAACTGGTAATTTTATTGCTTTTTTTCGACTTGCAAGGGAAGCAAGTTACCTCCAAGCATGCCTAATGCATGCTCACTTCTCAAAGTTACGAACCCAGGCACTTGCTTCTATACACGCAGGTCTCCAGAATAACCAAGGTCTCCCTATTGCGGATGCTGCCAAGTGGCTGGCAATGGAGGAAGATGAAATTGAAAGCCTTTCAGAGTATCATGGGTTTGTAATAAAGTCATTCCAGGAGCCATATATTGTGAAGGAAGGTCCATTTCTCAATGGTGATGAGGATTATCCTACCAAGTGTGCTAAACTTGTTGATATGAAAAAGTCAAGAAGGATAGTAGAGGATGTTTTGGCTTCTAGTCAAGTCATACCCTTGTCTTCTAAAGCAACAAAAAAGACTCAGTTGACTAAGTCAAATAAGCCTGAACCAAAAGTTATTTCATATGTTGAAAAGAAAAATCCTGTTCGTCGTGCCCCAGCCATTGAAGTTACAAAACCTGTCCATGAAGTTGATGAAGAAATGCCTAATTTTGAAGCTTTTTCACCCCCAAAAGATGCCAGACAAAAGCAGCAAATGATTCAAACACCAATTTTTGGTCAATACAGTAAAGATTCCCCTAAGGTGGCTGCTGTGTCTCCTTCCCTGTGGGATAAAGTTGGTACCATTGAGAAGCGAAACTATGATGCTCCTTTTAGTAACTCTCCAGAGACAAGCATGCATTCTGGCATGGAACGAATGCCACTGCAAATTGAGTCAAAAACATATGTACAAGAGAGATCTGTTGGTATATATAGTTCCGGGAAGGAATACCCAGATATCCAAATTATGGTAACTGATAAGTTGGAAGACGAGGAACCTCCAGATCTTCATCAGAAAGATGAAAACAATGATGTTATGGAGAGTTCTCAACAGGAAGAAATTGCGGAGGCAAAACTTAAGTTGATACTGAGATTATGGACGTGGCGTGCTCGGAAGCTAAGAGAATTGCGAGAGAAAAAACAGTTGGCAGCAATTGCTGCATTGAACTCTTTATCATTGGGGCCACCAATTCAACTCAAGACAGATCAACCAAGCACTTCTGGCGAGTTTGACATTGATCTCGTCTTGAGGGAGAGATATAAAAACCATGGTCAGTCATGGTCAAGACTTAATGTTTCAGATGTAATAGCGGATATACTGGGCACAAGAAATCCGGATGCTAGATGCCTCTGTTGGAAAACTGTTTTATGTTCTCAGATGAACAATCTAGAAGGTGATGACCTAGGGCAGAGGAGCCATGTCTTGGGAGCAGCCCCCTGGTTGCTTTCAAAGCTCATGCCTTCAGCAAATGATGTGGACAATGATGATGAGGATCTTGTAGTTTCATCTCCTGGTGTCTCTGTATGGAAGAAATGGGTTCGTGGCCAATCTGGTTCAGATCTGAATTGCTATTTGTCTGTGGTCAGGGATGCAAGTTTTGATAACCTATGTCAGAGTGTATCTGGTGCAAGTGCTGTTTTGTTCCTTACATCTGACAGCATCCCTTGGAATTTGCAAAAAGTCCGTCTTCATAACCTTCTGATGTCGATACCTTATGGTTCCTGCTTGCCCCTTCTGATCTTAAGTGGCTCTTACAAGAACAATGTTAATGACTCCACTATTGTTGATAATTTGGGTCTCCATGACTTGGATAAGTCACGAATAAGCAGCTTTCTGGTTGTTCCGCTTGTTGAAAGCCAGCAAATGGGACGATTGGATGGTTTTTTCAGTGACAACCGACTTAGGGAGGGGCTCCGGTGGCTTGCAAGTGAATCGCCCCTTCAACCCATTCTTCATCATGTGAAAACACGCGAACTCGTTCTTACCCACTTGAATTCTTCACTGGAGCCACTAGGCAGAATGAAAGATTATGAAGCAGGTCCAGACAACTGTATCCTTGCCTTCAATGAAGCATTGGATTGGTCACAGAAGGAAATTGCTGCTGCTGTCAAATCAAATCCATCCAGTTGGCCTTGCCCTGAGATTGCTTTGCTGGAGGAGTTCAGTGATGAGTACAGGGTTGTGAAATGGTGCTTGCCAAGCGTAGGATGGAGCTCAGCGGAAAAAGTTGAACCGCTAATGTCAGCACTAGGGGATTGTAGGCTTCTAGCTTTTCCCGACCATATTTCGTGGCTACCCCAATGTTGTAATGCAGGAAAGGAGATTGAGAACCTGAGAGGTGAACTTGAAAATGGCTTGATCGAGTACCTGACGAATTCAAGTAAGATGATGGGACTTGCACTTGCTATAAAAGAGGCACCTGTAATGCTACAAAGAAGCTGTCGGCTGGAGCGCCATGACTCGTGCTGCTATATAGTCCCGAATTGGGTTATGATCTTCAGACGAATTTTCAACTGGCGGTTAATGGGTTTAGCCAATGGGGCCTTTTTGTCAGCTTATGTATTGGAGAGTCCTCATCTTAACGCAGTTTTTGAAAATCTGGGTAAGTTGGGGCTTGAAGATACCGGACCTTCGCCCTGTTACCTTAATCAGCTGTCTCTGGATGAAATCCTCGAAGTTGGGCGCGCCCCTCTTTCATCCCATAGAGGTCAGCCCTTGGTAGAATCTGGTGTAGCTGTTCCTGAAACATCTCCTAATGGTGAAATCCACGAGACCCCGAATAGAACGGACTGGATGGAGGATGAACGAAGTTTAGCGCACAATGGTCAAGCGGAAATAGAAAACGTACCCCACGAAAATGGGAGGTTGGAGAATGCTGGCAGAGAAATGGTGGTGACTGGAGAAGTGGGCAAGGGGGCTGAAAAATTAAGCATATTGTTAGAACAGTGTAACATGTTACAGAATGTGATAGACGAGAAGCTGTCCATTTTTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008150 GO:0010468 GO:0019219 GO:0019222 GO:0031323 GO:0031974 GO:0031981 GO:0034399 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044030 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051252 GO:0060147 GO:0060255 GO:0060966 GO:0060968 GO:0065007 GO:0070013 GO:0070920 GO:0080090 GO:0090065 GO:1900368

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM3AP_GANP SAC3_GANP

Protein Analysis

1054

Amino Acids

118.38

Weight (kDa)

5.35

Isoelectric Point (pI)

51.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SAC3_GANP	PF03399	4 - 178	5.9e-37	SAC3/GANP family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06290 AT3G06290 AT3G06290 AT3G06290
fragaria_vesca	FvH4_4g13920 FvH4_4g13920 FvH4_4g13920 FvH4_4g13920 FvH4_4g13920
malus_domestica	MD13G1178600.v1.1 MD16G1179800.v1.1
prunus_persica	Prupe.1G147400_v2.0.a1
pyrus_communis	pycom13g15420 pycom16g15090
rosa_chinensis	RchiOBHm_Chr4g0419781
rosa_laevigata	RLG00000007798
rosa_multiflora	Rmu_sc0016868.1_g000004
rosa_roxburghii	Rroxscaffold_5G00362580
rosa_rugosa	Rorug04G0160400
rosa_samantha	Rh4AG221500 Rh4BG223500 Rh4CG234700 Rh4DG220600
rosa_wichuraiana	Rw4G019060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	223, 416, 2192
Acc65I	GGTACC	1 cut(s)	965
AccB1I	GGYRCC	2 cut(s)	965, 2581
AccB7I	CCANNNNNTGG	5 cut(s)	899, 1132, 1588, 1972, 2029
AccII	CGCG	2 cut(s)	2136, 2850
AccIII	TCCGGA	1 cut(s)	1498
AciI	CCGC	8 cut(s)	458, 1229, 1475, 2009, 2384, 2399, 2683, 2996
AclWI	GGATC	1 cut(s)	1665
AcoI	YGGCCR	1 cut(s)	1708
AcsI	RAATTY	4 cut(s)	1834, 2158, 2539, 2670
AcuI	CTGAAG	3 cut(s)	252, 1611, 2647
AcvI	CACGTG	1 cut(s)	287
AfaI	GTAC	6 cut(s)	203, 967, 1074, 2339, 2531, 3012
AflII	CTTAAG	2 cut(s)	1241, 1906
AflIII	ACRYGT	1 cut(s)	3117
AjiI	CACGTC	1 cut(s)	1266
AjnI	CCWGG	5 cut(s)	196, 402, 540, 1601, 1675
AjuI	GAANNNNNNNTTGG	4 cut(s)	727, 759, 1292, 1324
Alw21I	GWGCWC	3 cut(s)	225, 1276, 2381
Alw26I	GTCTC	8 cut(s)	434, 452, 945, 1007, 1687, 1967, 2826, 2930
AlwI	GGATC	1 cut(s)	1665
Aor13HI	TCCGGA	1 cut(s)	1498
AoxI	GGCC	4 cut(s)	1346, 1708, 2294, 2706
ApoI	RAATTY	4 cut(s)	1834, 2158, 2539, 2670
ArsI	GACNNNNNNTTYG	4 cut(s)	1834, 1866, 2590, 2622
AscI	GGCGCGCC	1 cut(s)	2848
Asp700I	GAANNNNTTC	1 cut(s)	2142
Asp718I	GGTACC	1 cut(s)	965
AspA2I	CCTAGG	1 cut(s)	1567
AspLEI	GCGC	4 cut(s)	2619, 2850, 2852, 2983
AspS9I	GGNCC	5 cut(s)	566, 1346, 2204, 2706, 2792
AsuC2I	CCSGG	1 cut(s)	1105
AsuHPI	GGTGA	6 cut(s)	593, 840, 1571, 2516, 2936, 3070
AvaII	GGWCC	3 cut(s)	566, 2204, 2792
AvrII	CCTAGG	1 cut(s)	1567
AxyI	CCTNAGG	1 cut(s)	924
BaeGI	GKGCMC	2 cut(s)	781, 1490
BaeI	ACNNNNGTAYC	2 cut(s)	2200, 2233
BalI	TGGCCA	1 cut(s)	1710
BanI	GGYRCC	2 cut(s)	965, 2581
BanII	GRGCYC	3 cut(s)	225, 2079, 2381
BauI	CACGAG	2 cut(s)	2626, 2933
BbrPI	CACGTG	1 cut(s)	287
BbsI	GAAGAC	3 cut(s)	675, 1158, 1844
Bbv12I	GWGCWC	3 cut(s)	225, 1276, 2381
BbvCI	CCTCAGC	1 cut(s)	42
BccI	CCATC	5 cut(s)	2036, 2293, 2367, 2548, 2953
BcgI	CGANNNNNNTGC	4 cut(s)	207, 241, 2079, 2113
BciT130I	CCWGG	5 cut(s)	198, 404, 542, 1603, 1677
BciVI	GTATCC	1 cut(s)	2228
BclI	TGATCA	1 cut(s)	115
BcnI	CCSGG	1 cut(s)	1105
BcoDI	GTCTC	8 cut(s)	434, 452, 945, 1007, 1687, 1967, 2826, 2930
BfaI	CTAG	8 cut(s)	297, 663, 1506, 1553, 1568, 2177, 2414, 2432
BfmI	CTRYAG	2 cut(s)	144, 216
BfoI	RGCGCY	1 cut(s)	2620
BfrI	CTTAAG	2 cut(s)	1241, 1906
BfuAI	ACCTGC	3 cut(s)	223, 416, 2192
BfuI	GTATCC	1 cut(s)	2228
BglI	GCCNNNNNGGC	1 cut(s)	2705
BglII	AGATCT	3 cut(s)	1082, 1168, 1723
BlnI	CCTAGG	1 cut(s)	1567
BlpI	GCTNAGC	1 cut(s)	2380
Bme1390I	CCNGG	6 cut(s)	198, 404, 542, 1105, 1603, 1677
Bme18I	GGWCC	3 cut(s)	566, 2204, 2792
BmgBI	CACGTC	1 cut(s)	1266
BmgT120I	GGNCC	5 cut(s)	566, 1346, 2204, 2706, 2792
BmrFI	CCNGG	6 cut(s)	198, 404, 542, 1105, 1603, 1677
BmrI	ACTGGG	1 cut(s)	1493
BmsI	GCATC	9 cut(s)	43, 202, 451, 850, 979, 1492, 1499, 1744, 1833
BmuI	ACTGGG	1 cut(s)	1493
BpiI	GAAGAC	3 cut(s)	675, 1158, 1844
BpmI	CTGGAG	8 cut(s)	234, 416, 993, 1149, 2189, 2339, 2633, 3084
Bpu10I	CCTNAGC	1 cut(s)	42
Bpu1102I	GCTNAGC	1 cut(s)	2380
BpuEI	CTTGAG	4 cut(s)	7, 164, 1346, 1432
BpuMI	CCSGG	1 cut(s)	1105
BsaAI	YACGTR	1 cut(s)	287
BsaBI	GATNNNNATC	2 cut(s)	1173, 1656
BsaI	GGTCTC	4 cut(s)	434, 452, 1967, 2930
BsaJI	CCNNGG	8 cut(s)	197, 402, 442, 1432, 1567, 1601, 1829, 2880
BsaWI	WCCGGW	3 cut(s)	1498, 2079, 2789
Bse1I	ACTGG	7 cut(s)	316, 1488, 2172, 2289, 2684, 2960, 3067
Bse21I	CCTNAGG	1 cut(s)	924
Bse3DI	GCAATG	1 cut(s)	486
Bse8I	GATNNNNATC	2 cut(s)	1173, 1656
BseAI	TCCGGA	1 cut(s)	1498
BseBI	CCWGG	5 cut(s)	198, 404, 542, 1603, 1677
BseDI	CCNNGG	8 cut(s)	197, 402, 442, 1432, 1567, 1601, 1829, 2880
BseJI	GATNNNNATC	2 cut(s)	1173, 1656
BseMI	GCAATG	1 cut(s)	486
BseMII	CTCAG	7 cut(s)	33, 719, 1244, 1553, 2295, 2394, 2489
BseNI	ACTGG	7 cut(s)	316, 1488, 2172, 2289, 2684, 2960, 3067
BsePI	GCGCGC	1 cut(s)	2848
BseRI	GAGGAG	3 cut(s)	42, 1591, 2336
BseSI	GKGCMC	2 cut(s)	781, 1490
BseYI	CCCAGC	1 cut(s)	781
Bsh1236I	CGCG	2 cut(s)	2136, 2850
BshFI	GGCC	4 cut(s)	1348, 1710, 2296, 2708
BshNI	GGYRCC	2 cut(s)	965, 2581
BsiHKAI	GWGCWC	3 cut(s)	225, 1276, 2381
BsiSI	CCGG	4 cut(s)	1104, 1499, 2080, 2790
BslFI	GGGAC	3 cut(s)	2046, 2571, 2627
BsmAI	GTCTC	8 cut(s)	434, 452, 945, 1007, 1687, 1967, 2826, 2930
BsmFI	GGGAC	3 cut(s)	2046, 2571, 2627
BsmI	GAATGC	3 cut(s)	1024, 1047, 3043
BsnI	GGCC	4 cut(s)	1348, 1710, 2296, 2708
Bso31I	GGTCTC	4 cut(s)	434, 452, 1967, 2930
Bsp1286I	GDGCHC	6 cut(s)	225, 781, 1276, 1490, 2079, 2381
Bsp13I	TCCGGA	1 cut(s)	1498
Bsp1407I	TGTACA	1 cut(s)	1072
Bsp1720I	GCTNAGC	1 cut(s)	2380
Bsp19I	CCATGG	1 cut(s)	1432
BspACI	CCGC	8 cut(s)	458, 1229, 1475, 2009, 2384, 2399, 2683, 2996
BspANI	GGCC	4 cut(s)	1348, 1710, 2296, 2708
BspCNI	CTCAG	7 cut(s)	34, 718, 1245, 1552, 2296, 2393, 2490
BspEI	TCCGGA	1 cut(s)	1498
BspFNI	CGCG	2 cut(s)	2136, 2850
BspMAI	CTGCAG	1 cut(s)	220
BspMI	ACCTGC	3 cut(s)	223, 416, 2192
BspPI	GGATC	1 cut(s)	1665
BspT107I	GGYRCC	2 cut(s)	965, 2581
BspTI	CTTAAG	2 cut(s)	1241, 1906
BspTNI	GGTCTC	4 cut(s)	434, 452, 1967, 2930
BsrDI	GCAATG	1 cut(s)	486
BsrGI	TGTACA	1 cut(s)	1072
BsrI	ACTGG	7 cut(s)	316, 1488, 2172, 2289, 2684, 2960, 3067
BssECI	CCNNGG	8 cut(s)	197, 402, 442, 1432, 1567, 1601, 1829, 2880
BssHII	GCGCGC	1 cut(s)	2848
BssSI	CACGAG	2 cut(s)	2626, 2933
BssT1I	CCWWGG	5 cut(s)	442, 1432, 1567, 1829, 2880
Bst2BI	CACGAG	2 cut(s)	2626, 2933
Bst2UI	CCWGG	5 cut(s)	198, 404, 542, 1603, 1677
Bst4CI	ACNGT	5 cut(s)	911, 1308, 1528, 2216, 3111
BstAFI	CTTAAG	2 cut(s)	1241, 1906
BstAPI	GCANNNNNTGC	2 cut(s)	1319, 1322
BstAUI	TGTACA	1 cut(s)	1072
BstBAI	YACGTR	1 cut(s)	287
BstDSI	CCRYGG	1 cut(s)	1432
BstFNI	CGCG	2 cut(s)	2136, 2850
BstH2I	RGCGCY	1 cut(s)	2620
BstHHI	GCGC	4 cut(s)	2619, 2850, 2852, 2983
BstMAI	GTCTC	8 cut(s)	434, 452, 945, 1007, 1687, 1967, 2826, 2930
BstNI	CCWGG	5 cut(s)	198, 404, 542, 1603, 1677
BstNSI	RCATGY	4 cut(s)	369, 380, 1024, 3121
BstSCI	CCNGG	6 cut(s)	196, 402, 540, 1103, 1601, 1675
BstSFI	CTRYAG	2 cut(s)	144, 216
BstSLI	GKGCMC	2 cut(s)	781, 1490
BstUI	CGCG	2 cut(s)	2136, 2850
BstV2I	GAAGAC	3 cut(s)	675, 1158, 1844
BstX2I	RGATCY	4 cut(s)	1082, 1168, 1657, 1723
BstXI	CCANNNNNNTGG	3 cut(s)	475, 2292, 3023
BstYI	RGATCY	4 cut(s)	1082, 1168, 1657, 1723
Bsu36I	CCTNAGG	1 cut(s)	924
BsuI	GTATCC	1 cut(s)	2228
BsuRI	GGCC	4 cut(s)	1348, 1710, 2296, 2708
BtgI	CCRYGG	1 cut(s)	1432
BtrI	CACGTC	1 cut(s)	1266
BtsI	GCAGTG	1 cut(s)	1046
BtsIMutI	CAGTG	5 cut(s)	1046, 2059, 2165, 2335, 3116
BveI	ACCTGC	3 cut(s)	223, 416, 2192
CfoI	GCGC	4 cut(s)	2619, 2850, 2852, 2983
Cfr13I	GGNCC	5 cut(s)	566, 1346, 2204, 2706, 2792
Csp6I	GTAC	6 cut(s)	202, 966, 1073, 2338, 2530, 3011
CspCI	CAANNNNNGTGG	4 cut(s)	1620, 1655, 1930, 1965
CviQI	GTAC	6 cut(s)	202, 966, 1073, 2338, 2530, 3011
EaeI	YGGCCR	1 cut(s)	1708
Ecl136II	GAGCTC	2 cut(s)	223, 2379
Eco130I	CCWWGG	5 cut(s)	442, 1432, 1567, 1829, 2880
Eco24I	GRGCYC	3 cut(s)	225, 2079, 2381
Eco31I	GGTCTC	4 cut(s)	434, 452, 1967, 2930
Eco32I	GATATC	1 cut(s)	1123
Eco47I	GGWCC	3 cut(s)	566, 2204, 2792
Eco53kI	GAGCTC	2 cut(s)	223, 2379
Eco57I	CTGAAG	3 cut(s)	252, 1611, 2647
Eco72I	CACGTG	1 cut(s)	287
Eco81I	CCTNAGG	1 cut(s)	924
EcoICRI	GAGCTC	2 cut(s)	223, 2379
EcoO109I	RGGNCCY	1 cut(s)	2706
EcoRI	GAATTC	2 cut(s)	2158, 2539
EcoRII	CCWGG	5 cut(s)	196, 402, 540, 1601, 1675
EcoRV	GATATC	1 cut(s)	1123
EcoT14I	CCWWGG	5 cut(s)	442, 1432, 1567, 1829, 2880
EcoT22I	ATGCAT	3 cut(s)	6, 378, 1026
EcoT38I	GRGCYC	3 cut(s)	225, 2079, 2381
ErhI	CCWWGG	5 cut(s)	442, 1432, 1567, 1829, 2880
FalI	AAGNNNNNCTT	6 cut(s)	250, 282, 1836, 1868, 1900, 1932
FaqI	GGGAC	3 cut(s)	2046, 2571, 2627
FauNDI	CATATG	2 cut(s)	748, 1069
FbaI	TGATCA	1 cut(s)	115
FriOI	GRGCYC	3 cut(s)	225, 2079, 2381
FspBI	CTAG	8 cut(s)	297, 663, 1506, 1553, 1568, 2177, 2414, 2432
GlaI	GCGC	4 cut(s)	2618, 2849, 2851, 2982
GsaI	CCCAGC	1 cut(s)	785
GsuI	CTGGAG	8 cut(s)	234, 416, 993, 1149, 2189, 2339, 2633, 3084
HaeII	RGCGCY	1 cut(s)	2620
HaeIII	GGCC	4 cut(s)	1348, 1710, 2296, 2708
HapII	CCGG	4 cut(s)	1104, 1499, 2080, 2790
HhaI	GCGC	4 cut(s)	2619, 2850, 2852, 2983
Hin6I	GCGC	4 cut(s)	2617, 2848, 2850, 2981
HinP1I	GCGC	4 cut(s)	2617, 2848, 2850, 2981
HincII	GTYRAC	1 cut(s)	711
HindII	GTYRAC	1 cut(s)	711
HindIII	AAGCTT	1 cut(s)	840
HpaII	CCGG	4 cut(s)	1104, 1499, 2080, 2790
HphI	GGTGA	6 cut(s)	593, 840, 1571, 2516, 2936, 3070
Hpy166II	GTNNAC	3 cut(s)	711, 1642, 2507
Hpy8I	GTNNAC	3 cut(s)	711, 1642, 2507
Hpy99I	CGWCG	1 cut(s)	777
HpyCH4III	ACNGT	5 cut(s)	911, 1308, 1528, 2216, 3111
HpyCH4IV	ACGT	3 cut(s)	286, 1265, 3009
HpySE526I	ACGT	3 cut(s)	286, 1265, 3009
HspAI	GCGC	4 cut(s)	2617, 2848, 2850, 2981
Kpn2I	TCCGGA	1 cut(s)	1498
KpnI	GGTACC	1 cut(s)	969
Ksp22I	TGATCA	1 cut(s)	115
LmnI	GCTCC	8 cut(s)	544, 997, 1578, 1592, 2082, 2170, 2376, 2614
LweI	GCATC	9 cut(s)	43, 202, 451, 850, 979, 1492, 1499, 1744, 1833
MaeI	CTAG	8 cut(s)	297, 663, 1506, 1553, 1568, 2177, 2414, 2432
MaeII	ACGT	3 cut(s)	286, 1265, 3009
MfeI	CAATTG	2 cut(s)	104, 1317
MflI	RGATCY	4 cut(s)	1082, 1168, 1657, 1723
MhlI	GDGCHC	6 cut(s)	225, 781, 1276, 1490, 2079, 2381
MlsI	TGGCCA	1 cut(s)	1710
MluNI	TGGCCA	1 cut(s)	1710
MlyI	GAGTC	6 cut(s)	241, 697, 1067, 1931, 2618, 2743
MmeI	TCCRAC	3 cut(s)	1128, 1499, 3012
Mox20I	TGGCCA	1 cut(s)	1710
Mph1103I	ATGCAT	3 cut(s)	6, 378, 1026
MroI	TCCGGA	1 cut(s)	1498
MroXI	GAANNNNTTC	1 cut(s)	2142
MscI	TGGCCA	1 cut(s)	1710
MseI	TTAA	8 cut(s)	1242, 1455, 1907, 1932, 2687, 2745, 2811, 3092
MslI	CAYNNNNRTG	1 cut(s)	3021
Msp20I	TGGCCA	1 cut(s)	1710
MspA1I	CMGCKG	2 cut(s)	2384, 2818
MspCI	CTTAAG	2 cut(s)	1241, 1906
MspI	CCGG	4 cut(s)	1104, 1499, 2080, 2790
MspR9I	CCNGG	6 cut(s)	198, 404, 542, 1105, 1603, 1677
MunI	CAATTG	2 cut(s)	104, 1317
Mva1269I	GAATGC	3 cut(s)	1024, 1047, 3043
MvaI	CCWGG	5 cut(s)	198, 404, 542, 1603, 1677
MvnI	CGCG	2 cut(s)	2136, 2850
NciI	CCSGG	1 cut(s)	1105
NcoI	CCATGG	1 cut(s)	1432
NdeI	CATATG	2 cut(s)	748, 1069
NmuCI	GTSAC	4 cut(s)	1980, 2054, 2247, 3058
NsiI	ATGCAT	3 cut(s)	6, 378, 1026
NspI	RCATGY	4 cut(s)	369, 380, 1024, 3121
PaeI	GCATGC	3 cut(s)	369, 380, 1024
PalAI	GGCGCGCC	1 cut(s)	2848
PauI	GCGCGC	1 cut(s)	2848
PciI	ACATGT	1 cut(s)	3117
PcsI	WCGNNNNNNNCGW	1 cut(s)	3015
PctI	GAATGC	3 cut(s)	1024, 1047, 3043
PdmI	GAANNNNTTC	1 cut(s)	2142
PfeI	GAWTC	4 cut(s)	883, 917, 2096, 2888
PflFI	GACNNNGTC	1 cut(s)	715
PflMI	CCANNNNNTGG	5 cut(s)	899, 1132, 1588, 1972, 2029
PfoI	TCCNGGA	2 cut(s)	540, 1103
PleI	GAGTC	6 cut(s)	241, 697, 1066, 1931, 2618, 2742
PmaCI	CACGTG	1 cut(s)	287
PmlI	CACGTG	1 cut(s)	287
PpsI	GAGTC	6 cut(s)	241, 697, 1066, 1931, 2618, 2742
Ppu21I	YACGTR	1 cut(s)	287
PscI	ACATGT	1 cut(s)	3117
Psp124BI	GAGCTC	2 cut(s)	225, 2381
Psp6I	CCWGG	5 cut(s)	196, 402, 540, 1601, 1675
PspCI	CACGTG	1 cut(s)	287
PspFI	CCCAGC	1 cut(s)	781
PspGI	CCWGG	5 cut(s)	196, 402, 540, 1601, 1675
PspPI	GGNCC	5 cut(s)	566, 1346, 2204, 2706, 2792
PsrI	GAACNNNNNNTAC	2 cut(s)	1084, 1116
PstI	CTGCAG	1 cut(s)	220
PsuI	RGATCY	4 cut(s)	1082, 1168, 1657, 1723
PsyI	GACNNNGTC	1 cut(s)	715
PteI	GCGCGC	1 cut(s)	2848
PvuII	CAGCTG	1 cut(s)	2818
RsaI	GTAC	6 cut(s)	203, 967, 1074, 2339, 2531, 3012
RsaNI	GTAC	6 cut(s)	202, 966, 1073, 2338, 2530, 3011
RseI	CAYNNNNRTG	1 cut(s)	3021
SacI	GAGCTC	2 cut(s)	225, 2381
SaqAI	TTAA	8 cut(s)	1242, 1455, 1907, 1932, 2687, 2745, 2811, 3092
Sau96I	GGNCC	5 cut(s)	566, 1346, 2204, 2706, 2792
SchI	GAGTC	6 cut(s)	241, 697, 1067, 1931, 2618, 2743
ScrFI	CCNGG	6 cut(s)	198, 404, 542, 1105, 1603, 1677
SduI	GDGCHC	6 cut(s)	225, 781, 1276, 1490, 2079, 2381
SfaNI	GCATC	9 cut(s)	43, 202, 451, 850, 979, 1492, 1499, 1744, 1833
SfcI	CTRYAG	2 cut(s)	144, 216
SgsI	GGCGCGCC	1 cut(s)	2848
SinI	GGWCC	3 cut(s)	566, 2204, 2792
SmiMI	CAYNNNNRTG	1 cut(s)	3021
SmlI	CTYRAG	6 cut(s)	22, 179, 1241, 1361, 1411, 1906
SmoI	CTYRAG	6 cut(s)	22, 179, 1241, 1361, 1411, 1906
SphI	GCATGC	3 cut(s)	369, 380, 1024
SsiI	CCGC	8 cut(s)	458, 1229, 1475, 2009, 2384, 2399, 2683, 2996
SspI	AATATT	1 cut(s)	67
SspMI	CTAG	8 cut(s)	297, 663, 1506, 1553, 1568, 2177, 2414, 2432
SstI	GAGCTC	2 cut(s)	225, 2381
StyD4I	CCNGG	6 cut(s)	196, 402, 540, 1103, 1601, 1675
StyI	CCWWGG	5 cut(s)	442, 1432, 1567, 1829, 2880
TaaI	ACNGT	5 cut(s)	911, 1308, 1528, 2216, 3111
TaiI	ACGT	3 cut(s)	289, 1268, 3012
TaqI	TCGA	5 cut(s)	160, 334, 1871, 2526, 2838
TatI	WGTACW	2 cut(s)	1072, 2337
TfiI	GAWTC	4 cut(s)	883, 917, 2096, 2888
Tru1I	TTAA	8 cut(s)	1242, 1455, 1907, 1932, 2687, 2745, 2811, 3092
Tru9I	TTAA	8 cut(s)	1242, 1455, 1907, 1932, 2687, 2745, 2811, 3092
TscAI	CASTG	5 cut(s)	1053, 2059, 2172, 2335, 3116
TseFI	GTSAC	4 cut(s)	1980, 2054, 2247, 3058
Tsp45I	GTSAC	4 cut(s)	1980, 2054, 2247, 3058
TspGWI	ACGGA	2 cut(s)	1838, 2966
TspRI	CASTG	5 cut(s)	1053, 2059, 2172, 2335, 3116
Tth111I	GACNNNGTC	1 cut(s)	715
Van91I	CCANNNNNTGG	5 cut(s)	899, 1132, 1588, 1972, 2029
Vha464I	CTTAAG	2 cut(s)	1241, 1906
VpaK11BI	GGWCC	3 cut(s)	566, 2204, 2792
XapI	RAATTY	4 cut(s)	1834, 2158, 2539, 2670
XbaI	TCTAGA	1 cut(s)	1552
XceI	RCATGY	4 cut(s)	369, 380, 1024, 3121
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2370
XmaJI	CCTAGG	1 cut(s)	1567
XmnI	GAANNNNTTC	1 cut(s)	2142
XspI	CTAG	8 cut(s)	297, 663, 1506, 1553, 1568, 2177, 2414, 2432
Zsp2I	ATGCAT	3 cut(s)	6, 378, 1026

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.