Rh4BG223500

SAC3/GANP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0007937
Physical Location & Seq
Reverse (-)
38634624 .. 38645486
10863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG223500.1

Sequence Viewer

Length: 5019 bp
ATGTTCTCAAGCTTCTCCAGTCAGGCAGGACCCACCGGACCTGCCGGAGAATCACCCATTCCAGTTCAATTCGGCACTTCCGGTCGCCGCCCTCCTTCACCTTCTCCGACTACCCCTCCTTTTCCCTACTCTGCCCCTCTGCGATCTCCTTCCGGTGGACCTGAAGCTCTAGGGCAACCGCATTTGGCATTTCAAAGTTCTCATCCCGGTGGAACTCCTTCGTATCGATCGGCAGGAGTGCAAAGGTCCCCTGAATCTCTTCCTAGTTGGAATGGTGGGCAGAGACCCTTGTTAAAAAATAATGATACCAAGGTTCATCAAAGGCCATCTGATGTCACATCATTTGTTGTTTCTCGTAATTCTGGAACTAGTGTTACAGCCAAGGTTGCCAGATTCCAAGACACGCGAGGCACCAGATCCCCTCCCTTATTATCAAGAGATGTAAATATAAGAAATTCCACTCAAAGTGTTCCTAGAAGTCACGTAGTTTCTCCAAGAACGCAATCACCTGCCTCGATTTCATATAATTATCAGCCTGTTGAGGATTTCAATCATGTTGGTGCAGTTGAAGGTCACTTAGTTCCTCCAAGAACACGGTCACCTCCCTCGGCATCTTATAACTATCATCCTGTTGAGGATTTGGATCGTTTTGGTGGAGTTGAAGGTCACTTGGTTACTTCAAGAACACAATCACCTCCCTCAGGCTTCTATAACCATCATCCTGTTGAGGACTTTGATCATTCTGGTGGAGTTGAAAGGCATGCATTTTCCTCTTCTGGACTGAATAGTTCGCCGAAGTTGATAGTTGATCATGCCAGATTGCAGGCTTATCAAGAACCATCTCGAGTATCACCTTCTGTCAGCACTTTTGACTCTGGTAGAAGAACTCCGGTCAACTATGATGATGTTCAAGTCCTGAAACGAACTAGGCCTTCTGTATCTCCTATTGGGTCAAATGCTACTTCAAGTGCAGTTTTCAGTACTCGTGATTCTCGGGTTCATCAACAGTCTTCAAACAATACTTTTTCTGAAGCTGCTGCAAACAATTTGACCACCATACCGGTTGCCAAAAGAATGAGGTCACCTCCTTTACTTCCTGATGATCAAGTCTTCAAGGGAAACTCATATGCCACTCAAGATGGTACTGAACGAGAAATGCAAGCCAAAGCAAAGCGATTAGCCCGCTTCAAGGTTGAATTAAGTAAAAGTCCACAAAGTGGTATTGATATTGTAGAGCGGGGGGTTTCTGCGACTAGAAATGAACAATCTAATGTTGAGAGGAACAGATCAGTTGCGTATAGTTCTACTCAATTGGCTAGGGATGTTACTGATGGCAATGCCTTATCCGAGTGTGAAGGTGTAGAACCGTCTGGTATCATTATTGGTGTATGCCCAGATATGTGTCCAGATTCAGAAAGGGCGGAGCGAGAAAGGAAAGGGGATCTTGATCGGCATGAGCGTGTGGATGGGGATAGAAATCAAACCAGCTTGTCCCTTGCTGTTAAAAAGTATAACAGGACAGCTGAGCGGGATGCAAACTTGATAAGACCGATGCCGATCCTGCAGAAGACAATGGATTATTTGCTCAAGTTGCTTGATACACCTTATAGCGATAGGTTTCTTAGCATATATAACTTCTTGTGGGATAGAATGCGGGCGATCCGAATGGACCTAAGAATGCAACACATTTTTAACCAAGAGGCTATTAATATGTTGGAGCAAATGATAAGACTTCACATAATTGCCATGCATGAATTATGTGAATACTCAAAAGGAGAGGGGTTTGCGGAAGGATTTGATGCTCACCTTAATATTGAGCAGATGAATAAAACGTCAGTAGAATTGTTCCAAATGTATGATGATCACAGAAAGAAAGGAATAAGCATTCCTACAGAAAAAGAGTTTCGGGGTTATTATGCGCTTCTCAAGCTGGACAAACATCCTGGGCATATGGTTGAACCTGCAGAGCTCTCACTTGATCTTGCAAAGATGACCCCAGAAATAAGACAAACTTCAGAGGTGCTATTTGCACGTGATGTAGCGAGAGCTTGTAGAACTGGTAATTTTATTGCCTTCTTTCGACTTGCAAGGAAAGCAACTTATCTTCAAGCATGCCTAATGCATGCTCATTTTGCAAAGTTAAGAACCCTGGCACTTGCTTCTCTACAAGCAGGGCTCCAGAATAACCAAGGTCTTCCTATTGCGGATGTTGCCAAGTGGCTAGCTATGGAGGAAGAGGAAATTGAAAGCCTTTCAGAGTATCATGGATTCCAAATGAAGTCTTACAATAAAGAGTCATATATGGTAAAGGAAGGTCCATTTCTAAATGGTGATGAGGAATATCCTACCAAGTGTTCTAAACTTGTTGACATGAAAAAATCAAGAAGGATAATCGAGGATGTTTTAGCTTCTGGTCAAGTCGTATCCTTGCCTCCGGAAGCTTCAAAAGGAATTCAGCTGACTAAACCAAATCAGCTTGGAGCAAAAACTGCTCCATATGGTGAAAGGGGAAGTCTTGTCCATGATGTGCTATCAATTGAAGTAGCAAACTCCATTCATGAAGTTGATGAAGAAATGGCTAATTATGAAGTCGTTTCATCCCCAAAAGATGTCAGGCAAAAGCAGAAAATAATTCCAACACCAATTTTTTCACCCCAAAAGGATGTCAGACAAAAGCAGCATATGATTCAAACACCAATTGCTCCGTCCCCAAAGGGTATCAGACAAAGGCAGCAGGTGATTAACATGCCAATTCTTGGTCCACACCATGAAGATGATAGTCAGATGGCTTCTGTGTCTCCTTCCCTGTGGGATCTCTCATCATATAAACCCCAGCCTGATAAAGTTGGTTTAAATGAGAAGCCCAACAAAGATGCTCTTTACAGTAGTTCTCCAGTGAAAAGCATGCATTTTGGCATGGAAGCAACGCCATTGCAGATTGGATCAAAAACATCTCCGCAATCTCCAGTTGGTACATATAGTTATGTTGCGCAACATTCAGTAGGCCAAATTGTGTCTAATAACTTGGAAAATGAGGAACCTACAGATCTTTGTCAGGACAATGAAAATGATGATGTTATGGGAGATTATCAACATGAGGAAATTGCCGAGGCAAAACTGAAGTTGATGTTAAGGTTATGGAGGCGGCGTTCTATGAAGCTAAGGGAACTGCGAGAGCAAAAGCAGTTGGCAGCAAATGCTGCATTGAGTTCTTTATCACTGGGACCACCAATTCAACTGAAGAGAGATCAACCACGCATGTCTGGTGACTTTGACATTGATCTTATTTTGAGAGAAAGACATCAAAAGCACGGCCAGTCCTGGTCCAGACTTAATGTTTCAGATGTAATAGCAGATACATTAAGCACAAGAAACCCAGATGCTAGATGCCTATGTTGGAAAGTTGTTGTATGTTCTCAGATGAACGATGTAGAAGGTGATGAACACTGGCAGAGGAACCATGCCCTGGAAGCAGCACCCTGGTTGCTTTCAAAGATCATGCCTTCAAAAAACGATCATGAAGATCTCTTAATTTCATCTCCTGGCGTGTCCATATGGAAGAAATGGGTTCAAGGCCAATCTGGTTCAGACCTTACCTGCTGTTTGTCTGTAGTTAAGGATGCAAGTAGTGATAATCTAATTGAGTGTGTATCTGGTATGAGTGCACTTTTGTTTCTTGTATCAGAAAGCATCCCTTGGAAATTGCAAAAAGCCCAGCTCCATAACCTTCTGATGTCAGTACCTTATGGTTCCTGCTTACCTCTTCTGATCCTAGCTGGTTCATTCAAGAATGACATTGCAGATCCTTCCTCCATTATAGTTGATAATATGGGTCTCCATGACTTCGATAAGTCCCGAATAAGCAGCTTTCGGATTGTTCCCCTTGTTGAAAACCAGAAAAGAGAGCAATTGGATGGGTTCTATAGTGACAACCGACTGAGGGAGGGGCTCAGATGGCTTGCAAGTGAATCACCTACTCAACCCATTCTTCATCATGTGAAAACACATGAATTGATTCTGACCCACTTGAATTCCTCATTGGAGGCACTAGAGAAAATGAAAGATTATGAAGTAGGTCCAAACAACTGTATCCTATCCTTCAATGAAGCTTTGGATCAGTCACAGAGGGAAATTGCTGCCGCTGCCGAAGCAAATCCTGCTAGTTTCCCTTGCCCTGAAATTGCTTTGCTGGAGGAGTACAGTGAAGAACACAGGCTGGTGAAGTGGTGCTTGCCAAGCATAGGATGGAGCTCAGTAGCAAAAATAGAACCACTAATGTCAGCTCTGGGGAATTGTAGACTTCCCACTTTTCCTAATACTATTTCATGGTTGCCCAGATGTTCATATGCAAGGAAGGAGATTGGGAGCCTGAAAGTTGAACTTGAAAATGGCTTGATCAGCTACCTTGCGGATTCTAAGATGATGGGACTTGAACTTGCTATAAAGGAGGCATATGTAATGCTACAAAGAAGTTGTCGACTTGAGTGCCATGACTCATGGTGCTACATTGTGCCCAAGTGGACTATGATTTTTCGACGAATCTTCAATTGGCGGTTGATGGGTTTGGCTAATGGGACATTCTCTTCAGCTTATATCCTGGAATGCCCTCATCTCAATGCAACTTTTGGAAACCTGGGTAAGTTGACGCTTGAAGATAGTGAACCTTCAGCTTATCATCTCAATCAGCCGACTCTGGATGAAGTCATTGAAGTTTGTCGCAGCCCTCTAATGTTTCAGAGAGGTCAGTCCTTGCAAGAAACTGATCCGACTCTTCCTGAGACATCACCAAATGGTTCAATTCTTGAGACTCCGAACACAGATGTTTTTATGGATGATGAGACACGCTTAACCGATGATATAGAGGATGTGCCTGACGTAAATAGGGGACTAGAAAATGGTGGCGGAGAACTGGTGGTGGCTGGGAAAGAGACCAAGGAAGATGATAAATTGAGTACATTGTTGGAGCACTGTAACATGTTACAAAATGTGATAGATAAGAAGTTGTCCATTTATTTCTAA

Protein Analysis

1672

Amino Acids

186.92

Weight (kDa)

6.14

Isoelectric Point (pI)

58.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 467 - 760 6.3e-82 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
AarI CACCTGC 2 cut(s) 517, 2725
Acc16I TGCGCA 1 cut(s) 2991
Acc36I ACCTGC 5 cut(s) 49, 517, 1969, 2725, 3605
AccB1I GGYRCC 1 cut(s) 410
AccB7I CCANNNNNTGG 3 cut(s) 1217, 2756, 3466
AccBSI CCGCTC 2 cut(s) 1237, 1528
AccI GTMKAC 2 cut(s) 4297, 4477
AccII CGCG 1 cut(s) 406
AccIII TCCGGA 1 cut(s) 2434
AcoI YGGCCR 1 cut(s) 3313
AcsI RAATTY 3 cut(s) 454, 2451, 4030
AcuI CTGAAG 7 cut(s) 183, 1050, 1998, 3140, 3260, 4569, 4650
AcvI CACGTG 1 cut(s) 2033
AdeI CACNNNGTG 1 cut(s) 1217
AfaI GTAC 6 cut(s) 982, 1144, 2974, 3741, 4199, 4954
AfiI CCNNNNNNNGG 8 cut(s) 155, 701, 1189, 1217, 2756, 3466, 3940, 4241
AflIII ACRYGT 1 cut(s) 4974
AgeI ACCGGT 1 cut(s) 1060
AhlI ACTAGT 1 cut(s) 368
AjnI CCWGG 8 cut(s) 1942, 2148, 3320, 3465, 3479, 3541, 4596, 4632
AjuI GAANNNNNNNTTGG 4 cut(s) 4022, 4054, 4531, 4563
AloI GAACNNNNNNTCC 2 cut(s) 3133, 3165
Alw21I GWGCWC 4 cut(s) 1971, 3667, 4253, 4968
Alw26I GTCTC 7 cut(s) 277, 2802, 3839, 4772, 4799, 4832, 4922
Alw44I GTGCAC 1 cut(s) 3663
Ama87I CYCGRG 2 cut(s) 843, 993
Aor13HI TCCGGA 1 cut(s) 2434
AoxI GGCC 5 cut(s) 323, 929, 3004, 3313, 3574
ApaLI GTGCAC 1 cut(s) 3663
ApoI RAATTY 3 cut(s) 454, 2451, 4030
ArsI GACNNNNNNTTYG 2 cut(s) 4459, 4491
AseI ATTAAT 1 cut(s) 1707
AsiGI ACCGGT 1 cut(s) 1060
Asp700I GAANNNNTTC 3 cut(s) 217, 258, 4014
AspLEI GCGC 2 cut(s) 1921, 2992
AsuC2I CCSGG 1 cut(s) 207
AsuNHI GCTAGC 1 cut(s) 2221
AvaI CYCGRG 2 cut(s) 843, 993
AxyI CCTNAGG 1 cut(s) 700
BaeGI GKGCMC 2 cut(s) 3667, 4515
BaeI ACNNNNGTAYC 4 cut(s) 297, 330, 4072, 4105
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 4 cut(s) 1971, 2178, 3951, 4253
BauI CACGAG 1 cut(s) 984
BbrPI CACGTG 1 cut(s) 2033
BbsI GAAGAC 4 cut(s) 1002, 1102, 1574, 2186
Bbv12I GWGCWC 4 cut(s) 1971, 3667, 4253, 4968
BceAI ACGGC 1 cut(s) 3328
BcgI CGANNNNNNTGC 2 cut(s) 4467, 4501
BciT130I CCWGG 8 cut(s) 1944, 2150, 3322, 3467, 3481, 3543, 4598, 4634
BciVI GTATCC 2 cut(s) 2434, 4100
BclI TGATCA 5 cut(s) 736, 808, 1102, 1861, 4395
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 7 cut(s) 277, 2802, 3839, 4772, 4799, 4832, 4922
BcuI ACTAGT 1 cut(s) 368
BfmI CTRYAG 6 cut(s) 1562, 1890, 1962, 3042, 3609, 3922
BfuAI ACCTGC 5 cut(s) 49, 517, 1969, 2725, 3605
BfuI GTATCC 2 cut(s) 2434, 4100
BglII AGATCT 2 cut(s) 3046, 3523
BlpI GCTNAGC 1 cut(s) 1524
BmcAI AGTACT 1 cut(s) 982
Bme1390I CCNGG 9 cut(s) 207, 1944, 2150, 3322, 3467, 3481, 3543, 4598, 4634
BmeT110I CYCGRG 2 cut(s) 843, 993
BmiI GGNNCC 9 cut(s) 31, 248, 412, 2177, 3039, 3225, 3458, 3751, 4367
BmrFI CCNGG 9 cut(s) 207, 1944, 2150, 3322, 3467, 3481, 3543, 4598, 4634
BmrI ACTGGG 1 cut(s) 3230
BmsI GCATC 9 cut(s) 620, 1522, 1542, 1789, 2863, 3370, 3377, 3610, 3699
BmtI GCTAGC 1 cut(s) 2225
BmuI ACTGGG 1 cut(s) 3230
BpiI GAAGAC 4 cut(s) 1002, 1102, 1574, 2186
BplI GAGNNNNNCTC 2 cut(s) 1069, 1101
BpmI CTGGAG 4 cut(s) 2162, 2877, 2949, 4211
Bpu10I CCTNAGC 1 cut(s) 3161
Bpu1102I GCTNAGC 1 cut(s) 1524
BpuEI CTTGAG 5 cut(s) 1119, 1571, 1910, 4502, 4823
BpuMI CCSGG 1 cut(s) 207
Bsa29I ATCGAT 1 cut(s) 226
BsaAI YACGTR 2 cut(s) 484, 2033
BsaBI GATNNNNATC 5 cut(s) 549, 642, 1446, 1476, 1556
BsaI GGTCTC 3 cut(s) 277, 3839, 4922
BsaWI WCCGGW 6 cut(s) 35, 80, 152, 889, 1060, 2434
BsaXI ACNNNNNCTCC 6 cut(s) 100, 130, 3133, 3163, 4896, 4926
Bsc4I CCNNNNNNNGG 8 cut(s) 155, 701, 1189, 1217, 2756, 3466, 3940, 4241
Bse118I RCCGGY 1 cut(s) 1060
Bse1I ACTGG 9 cut(s) 18, 62, 2062, 2894, 2966, 3225, 3316, 3452, 4914
Bse21I CCTNAGG 1 cut(s) 700
Bse3DI GCAATG 3 cut(s) 1342, 2930, 3795
Bse8I GATNNNNATC 5 cut(s) 549, 642, 1446, 1476, 1556
BseAI TCCGGA 1 cut(s) 2434
BseBI CCWGG 8 cut(s) 1944, 2150, 3322, 3467, 3481, 3543, 4598, 4634
BseCI ATCGAT 1 cut(s) 226
BseJI GATNNNNATC 5 cut(s) 549, 642, 1446, 1476, 1556
BseLI CCNNNNNNNGG 8 cut(s) 155, 701, 1189, 1217, 2756, 3466, 3940, 4241
BseMI GCAATG 3 cut(s) 1342, 2930, 3795
BseMII CTCAG 7 cut(s) 714, 1515, 3431, 3929, 3964, 4266, 4767
BseNI ACTGG 9 cut(s) 18, 62, 2062, 2894, 2966, 3225, 3316, 3452, 4914
BseRI GAGGAG 1 cut(s) 4208
BseSI GKGCMC 2 cut(s) 3667, 4515
BseYI CCCAGC 3 cut(s) 2832, 3714, 4919
BsgI GTGCAG 2 cut(s) 582, 990
Bsh1236I CGCG 1 cut(s) 406
Bsh1285I CGRYCG 2 cut(s) 85, 230
BshFI GGCC 5 cut(s) 325, 931, 3006, 3315, 3576
BshNI GGYRCC 1 cut(s) 410
BshTI ACCGGT 1 cut(s) 1060
BshVI ATCGAT 1 cut(s) 226
BsiEI CGRYCG 2 cut(s) 85, 230
BsiHKAI GWGCWC 4 cut(s) 1971, 3667, 4253, 4968
BsiHKCI CYCGRG 2 cut(s) 843, 993
BsiSI CCGG 8 cut(s) 36, 45, 81, 153, 207, 890, 1061, 2435
BslFI GGGAC 8 cut(s) 232, 1477, 2692, 3237, 3838, 4440, 4588, 4899
BslI CCNNNNNNNGG 8 cut(s) 155, 701, 1189, 1217, 2756, 3466, 3940, 4241
BsmAI GTCTC 7 cut(s) 277, 2802, 3839, 4772, 4799, 4832, 4922
BsmFI GGGAC 8 cut(s) 232, 1477, 2692, 3237, 3838, 4440, 4588, 4899
BsmI GAATGC 4 cut(s) 1656, 1683, 1884, 4607
BsnI GGCC 5 cut(s) 325, 931, 3006, 3315, 3576
Bso31I GGTCTC 3 cut(s) 277, 3839, 4922
BsoBI CYCGRG 2 cut(s) 843, 993
Bsp1286I GDGCHC 7 cut(s) 1971, 2178, 3667, 3951, 4253, 4515, 4968
Bsp13I TCCGGA 1 cut(s) 2434
Bsp1720I GCTNAGC 1 cut(s) 1524
BspANI GGCC 5 cut(s) 325, 931, 3006, 3315, 3576
BspCNI CTCAG 7 cut(s) 713, 1516, 3430, 3930, 3963, 4265, 4768
BspDI ATCGAT 1 cut(s) 226
BspEI TCCGGA 1 cut(s) 2434
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 2 cut(s) 2557, 3517
BspLI GGNNCC 9 cut(s) 31, 248, 412, 2177, 3039, 3225, 3458, 3751, 4367
BspMAI CTGCAG 2 cut(s) 1566, 1966
BspMI ACCTGC 5 cut(s) 49, 517, 1969, 2725, 3605
BspOI GCTAGC 1 cut(s) 2225
BspT107I GGYRCC 1 cut(s) 410
BspTNI GGTCTC 3 cut(s) 277, 3839, 4922
BsrBI CCGCTC 2 cut(s) 1237, 1528
BsrDI GCAATG 3 cut(s) 1342, 2930, 3795
BsrFI RCCGGY 1 cut(s) 1060
BsrI ACTGG 9 cut(s) 18, 62, 2062, 2894, 2966, 3225, 3316, 3452, 4914
BssAI RCCGGY 1 cut(s) 1060
BssSI CACGAG 1 cut(s) 984
BssT1I CCWWGG 5 cut(s) 309, 381, 2188, 3695, 4932
Bst2BI CACGAG 1 cut(s) 984
Bst2UI CCWGG 8 cut(s) 1944, 2150, 3322, 3467, 3481, 3543, 4598, 4634
Bst4CI ACNGT 7 cut(s) 597, 1008, 1368, 2885, 4088, 4202, 4970
Bst6I CTCTTC 7 cut(s) 264, 778, 2229, 3236, 3768, 4588, 4776
BstAPI GCANNNNNTGC 4 cut(s) 2489, 3197, 3200, 4157
BstBAI YACGTR 2 cut(s) 484, 2033
BstEII GGTNACC 2 cut(s) 597, 1080
BstENI CCTNNNNNAGG 1 cut(s) 699
BstFNI CGCG 1 cut(s) 406
BstHHI GCGC 2 cut(s) 1921, 2992
BstMAI GTCTC 7 cut(s) 277, 2802, 3839, 4772, 4799, 4832, 4922
BstMCI CGRYCG 2 cut(s) 85, 230
BstNI CCWGG 8 cut(s) 1944, 2150, 3322, 3467, 3481, 3543, 4598, 4634
BstNSI RCATGY 7 cut(s) 764, 2115, 2126, 2749, 2908, 3262, 4978
BstPI GGTNACC 2 cut(s) 597, 1080
BstSCI CCNGG 9 cut(s) 205, 1942, 2148, 3320, 3465, 3479, 3541, 4596, 4632
BstSFI CTRYAG 6 cut(s) 1562, 1890, 1962, 3042, 3609, 3922
BstSLI GKGCMC 2 cut(s) 3667, 4515
BstUI CGCG 1 cut(s) 406
BstV2I GAAGAC 4 cut(s) 1002, 1102, 1574, 2186
BstX2I RGATCY 6 cut(s) 416, 1441, 2812, 3046, 3523, 3802
BstYI RGATCY 6 cut(s) 416, 1441, 2812, 3046, 3523, 3802
Bsu15I ATCGAT 1 cut(s) 226
Bsu36I CCTNAGG 1 cut(s) 700
BsuI GTATCC 2 cut(s) 2434, 4100
BsuRI GGCC 5 cut(s) 325, 931, 3006, 3315, 3576
BsuTUI ATCGAT 1 cut(s) 226
BtsIMutI CAGTG 5 cut(s) 2901, 3218, 3445, 4207, 4966
BveI ACCTGC 5 cut(s) 49, 517, 1969, 2725, 3605
CciI TCATGA 2 cut(s) 2557, 3517
CfoI GCGC 2 cut(s) 1921, 2992
Cfr10I RCCGGY 1 cut(s) 1060
ClaI ATCGAT 1 cut(s) 226
CseI GACGC 1 cut(s) 4654
Csp6I GTAC 6 cut(s) 981, 1143, 2973, 3740, 4198, 4953
CspAI ACCGGT 1 cut(s) 1060
CviQI GTAC 6 cut(s) 981, 1143, 2973, 3740, 4198, 4953
DraI TTTAAA 1 cut(s) 2853
DraIII CACNNNGTG 1 cut(s) 1217
EaeI YGGCCR 1 cut(s) 3313
Eam1104I CTCTTC 7 cut(s) 264, 778, 2229, 3236, 3768, 4588, 4776
EarI CTCTTC 7 cut(s) 264, 778, 2229, 3236, 3768, 4588, 4776
EciI GGCGGA 2 cut(s) 1436, 4917
Ecl136II GAGCTC 2 cut(s) 1969, 4251
Eco130I CCWWGG 5 cut(s) 309, 381, 2188, 3695, 4932
Eco147I AGGCCT 1 cut(s) 931
Eco24I GRGCYC 4 cut(s) 1971, 2178, 3951, 4253
Eco31I GGTCTC 3 cut(s) 277, 3839, 4922
Eco53kI GAGCTC 2 cut(s) 1969, 4251
Eco57I CTGAAG 7 cut(s) 183, 1050, 1998, 3140, 3260, 4569, 4650
Eco72I CACGTG 1 cut(s) 2033
Eco81I CCTNAGG 1 cut(s) 700
Eco88I CYCGRG 2 cut(s) 843, 993
Eco91I GGTNACC 2 cut(s) 597, 1080
EcoICRI GAGCTC 2 cut(s) 1969, 4251
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoO109I RGGNCCY 2 cut(s) 29, 246
EcoO65I GGTNACC 2 cut(s) 597, 1080
EcoRI GAATTC 2 cut(s) 2451, 4030
EcoRII CCWGG 8 cut(s) 1942, 2148, 3320, 3465, 3479, 3541, 4596, 4632
EcoT14I CCWWGG 5 cut(s) 309, 381, 2188, 3695, 4932
EcoT22I ATGCAT 4 cut(s) 766, 1752, 2124, 2910
EcoT38I GRGCYC 4 cut(s) 1971, 2178, 3951, 4253
ErhI CCWWGG 5 cut(s) 309, 381, 2188, 3695, 4932
FaqI GGGAC 8 cut(s) 232, 1477, 2692, 3237, 3838, 4440, 4588, 4899
FauI CCCGC 4 cut(s) 1190, 1230, 1521, 1647
FauNDI CATATG 7 cut(s) 1126, 1950, 2497, 2682, 3554, 4345, 4453
FbaI TGATCA 5 cut(s) 736, 808, 1102, 1861, 4395
FblI GTMKAC 2 cut(s) 4297, 4477
FriOI GRGCYC 4 cut(s) 1971, 2178, 3951, 4253
FspI TGCGCA 1 cut(s) 2991
GlaI GCGC 2 cut(s) 1920, 2991
GsaI CCCAGC 3 cut(s) 2836, 3718, 4923
GsuI CTGGAG 4 cut(s) 2162, 2877, 2949, 4211
HaeIII GGCC 5 cut(s) 325, 931, 3006, 3315, 3576
HapII CCGG 8 cut(s) 36, 45, 81, 153, 207, 890, 1061, 2435
HgaI GACGC 1 cut(s) 4654
HhaI GCGC 2 cut(s) 1921, 2992
Hin6I GCGC 2 cut(s) 1919, 2990
HinP1I GCGC 2 cut(s) 1919, 2990
HincII GTYRAC 4 cut(s) 895, 2368, 4478, 4644
HindII GTYRAC 4 cut(s) 895, 2368, 4478, 4644
HindIII AAGCTT 3 cut(s) 10, 2439, 4107
HpaII CCGG 8 cut(s) 36, 45, 81, 153, 207, 890, 1061, 2435
Hpy99I CGWCG 1 cut(s) 4539
HpyCH4III ACNGT 7 cut(s) 597, 1008, 1368, 2885, 4088, 4202, 4970
HpyCH4IV ACGT 4 cut(s) 483, 1832, 2032, 4875
HpySE526I ACGT 4 cut(s) 483, 1832, 2032, 4875
HspAI GCGC 2 cut(s) 1919, 2990
Kpn2I TCCGGA 1 cut(s) 2434
Ksp22I TGATCA 5 cut(s) 736, 808, 1102, 1861, 4395
LweI GCATC 9 cut(s) 620, 1522, 1542, 1789, 2863, 3370, 3377, 3610, 3699
MaeII ACGT 4 cut(s) 483, 1832, 2032, 4875
MbiI CCGCTC 2 cut(s) 1237, 1528
MfeI CAATTG 5 cut(s) 1310, 2535, 2697, 3908, 4546
MflI RGATCY 6 cut(s) 416, 1441, 2812, 3046, 3523, 3802
MhlI GDGCHC 7 cut(s) 1971, 2178, 3667, 3951, 4253, 4515, 4968
MlyI GAGTC 6 cut(s) 866, 2303, 4487, 4684, 4762, 4801
MmeI TCCRAC 7 cut(s) 131, 248, 1695, 2660, 3377, 4790, 4941
Mph1103I ATGCAT 4 cut(s) 766, 1752, 2124, 2910
MroI TCCGGA 1 cut(s) 2434
MroXI GAANNNNTTC 3 cut(s) 217, 258, 4014
MslI CAYNNNNRTG 6 cut(s) 207, 558, 744, 1458, 2772, 4614
MspA1I CMGCKG 3 cut(s) 1523, 2458, 4142
MspI CCGG 8 cut(s) 36, 45, 81, 153, 207, 890, 1061, 2435
MspR9I CCNGG 9 cut(s) 207, 1944, 2150, 3322, 3467, 3481, 3543, 4598, 4634
MunI CAATTG 5 cut(s) 1310, 2535, 2697, 3908, 4546
Mva1269I GAATGC 4 cut(s) 1656, 1683, 1884, 4607
MvaI CCWGG 8 cut(s) 1944, 2150, 3322, 3467, 3481, 3543, 4598, 4634
MvnI CGCG 1 cut(s) 406
NciI CCSGG 1 cut(s) 207
NdeI CATATG 7 cut(s) 1126, 1950, 2497, 2682, 3554, 4345, 4453
NheI GCTAGC 1 cut(s) 2221
NlaIV GGNNCC 9 cut(s) 31, 248, 412, 2177, 3039, 3225, 3458, 3751, 4367
NmeAIII GCCGAG 2 cut(s) 587, 3133
NmuCI GTSAC 9 cut(s) 334, 479, 572, 597, 665, 1080, 3266, 3926, 4119
NsbI TGCGCA 1 cut(s) 2991
NsiI ATGCAT 4 cut(s) 766, 1752, 2124, 2910
NspI RCATGY 7 cut(s) 764, 2115, 2126, 2749, 2908, 3262, 4978
PaeI GCATGC 4 cut(s) 764, 2115, 2126, 2908
PaeR7I CTCGAG 1 cut(s) 843
PagI TCATGA 2 cut(s) 2557, 3517
PaqCI CACCTGC 2 cut(s) 517, 2725
PceI AGGCCT 1 cut(s) 931
PciI ACATGT 1 cut(s) 4974
PcsI WCGNNNNNNNCGW 1 cut(s) 1456
PctI GAATGC 4 cut(s) 1656, 1683, 1884, 4607
PdmI GAANNNNTTC 3 cut(s) 217, 258, 4014
PflMI CCANNNNNTGG 3 cut(s) 1217, 2756, 3466
PfoI TCCNGGA 1 cut(s) 4596
PinAI ACCGGT 1 cut(s) 1060
Ple19I CGATCG 1 cut(s) 230
PleI GAGTC 6 cut(s) 866, 2302, 4487, 4684, 4762, 4801
PmaCI CACGTG 1 cut(s) 2033
PmlI CACGTG 1 cut(s) 2033
PpsI GAGTC 6 cut(s) 866, 2302, 4487, 4684, 4762, 4801
Ppu21I YACGTR 2 cut(s) 484, 2033
PpuMI RGGWCCY 2 cut(s) 29, 246
PscI ACATGT 1 cut(s) 4974
PshBI ATTAAT 1 cut(s) 1707
PsiI TTATAA 1 cut(s) 618
Psp124BI GAGCTC 2 cut(s) 1971, 4253
Psp5II RGGWCCY 2 cut(s) 29, 246
Psp6I CCWGG 8 cut(s) 1942, 2148, 3320, 3465, 3479, 3541, 4596, 4632
PspCI CACGTG 1 cut(s) 2033
PspEI GGTNACC 2 cut(s) 597, 1080
PspFI CCCAGC 3 cut(s) 2832, 3714, 4919
PspGI CCWGG 8 cut(s) 1942, 2148, 3320, 3465, 3479, 3541, 4596, 4632
PspN4I GGNNCC 9 cut(s) 31, 248, 412, 2177, 3039, 3225, 3458, 3751, 4367
PspPPI RGGWCCY 2 cut(s) 29, 246
PsrI GAACNNNNNNTAC 2 cut(s) 358, 390
PstI CTGCAG 2 cut(s) 1566, 1966
PsuI RGATCY 6 cut(s) 416, 1441, 2812, 3046, 3523, 3802
PvuI CGATCG 1 cut(s) 230
PvuII CAGCTG 2 cut(s) 1523, 2458
RsaI GTAC 6 cut(s) 982, 1144, 2974, 3741, 4199, 4954
RsaNI GTAC 6 cut(s) 981, 1143, 2973, 3740, 4198, 4953
RseI CAYNNNNRTG 6 cut(s) 207, 558, 744, 1458, 2772, 4614
SacI GAGCTC 2 cut(s) 1971, 4253
SalI GTCGAC 1 cut(s) 4476
ScaI AGTACT 1 cut(s) 982
SchI GAGTC 6 cut(s) 866, 2303, 4487, 4684, 4762, 4801
ScrFI CCNGG 9 cut(s) 207, 1944, 2150, 3322, 3467, 3481, 3543, 4598, 4634
SduI GDGCHC 7 cut(s) 1971, 2178, 3667, 3951, 4253, 4515, 4968
SfaNI GCATC 9 cut(s) 620, 1522, 1542, 1789, 2863, 3370, 3377, 3610, 3699
SfcI CTRYAG 6 cut(s) 1562, 1890, 1962, 3042, 3609, 3922
Sfr274I CTCGAG 1 cut(s) 843
SlaI CTCGAG 1 cut(s) 843
SmiMI CAYNNNNRTG 6 cut(s) 207, 558, 744, 1458, 2772, 4614
SmlI CTYRAG 7 cut(s) 7, 843, 1134, 1586, 1925, 4481, 4802
SmoI CTYRAG 7 cut(s) 7, 843, 1134, 1586, 1925, 4481, 4802
SpeI ACTAGT 1 cut(s) 368
SphI GCATGC 4 cut(s) 764, 2115, 2126, 2908
SseBI AGGCCT 1 cut(s) 931
SspI AATATT 1 cut(s) 1813
SstI GAGCTC 2 cut(s) 1971, 4253
StuI AGGCCT 1 cut(s) 931
StyD4I CCNGG 9 cut(s) 205, 1942, 2148, 3320, 3465, 3479, 3541, 4596, 4632
StyI CCWWGG 5 cut(s) 309, 381, 2188, 3695, 4932
TaaI ACNGT 7 cut(s) 597, 1008, 1368, 2885, 4088, 4202, 4970
TaiI ACGT 4 cut(s) 486, 1835, 2035, 4878
TaqI TCGA 8 cut(s) 226, 515, 844, 2080, 2394, 3846, 4477, 4534
TaqII GACCGA 1 cut(s) 1564
TatI WGTACW 3 cut(s) 980, 4197, 4952
TauI GCSGC 3 cut(s) 90, 3148, 4142
TscAI CASTG 5 cut(s) 2901, 3225, 3452, 4207, 4973
TseFI GTSAC 9 cut(s) 334, 479, 572, 597, 665, 1080, 3266, 3926, 4119
Tsp45I GTSAC 9 cut(s) 334, 479, 572, 597, 665, 1080, 3266, 3926, 4119
TspGWI ACGGA 1 cut(s) 2694
TspRI CASTG 5 cut(s) 2901, 3225, 3452, 4207, 4973
Van91I CCANNNNNTGG 3 cut(s) 1217, 2756, 3466
VneI GTGCAC 1 cut(s) 3663
VspI ATTAAT 1 cut(s) 1707
XagI CCTNNNNNAGG 1 cut(s) 699
XapI RAATTY 3 cut(s) 454, 2451, 4030
XceI RCATGY 7 cut(s) 764, 2115, 2126, 2749, 2908, 3262, 4978
XcmI CCANNNNNNNNNTGG 1 cut(s) 4242
XhoI CTCGAG 1 cut(s) 843
XmiI GTMKAC 2 cut(s) 4297, 4477
XmnI GAANNNNTTC 3 cut(s) 217, 258, 4014
ZrmI AGTACT 1 cut(s) 982
Zsp2I ATGCAT 4 cut(s) 766, 1752, 2124, 2910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.