Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0007937

Physical Location & Seq

Genomic Coordinates Forward (+)

15424714 .. 15433901

Gene Structure 9188 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1179800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4632 bp

ATGTGGTCCACAGATTCTCATCCTACTTGGGATGGTGGGCAGCAATCCTCCTTTAAGAATTATGATACCCAGGCTCATCAAAGGACATCTGCCGTCACATCATTTGTTGTTTCTCGTAATTCTGGAACTAGTGTTACTGCCAAGGTGTCCAGATTCCAAGACACTGAAGAGATCAGATCGCCTCCCTTTCTGTCTAAAGATGTCAATTTCAGAAATTCCACTCAAGGTGTCCCTAGAAGTCACTTAGTTACTCCGAGAATACGATCGCCTCCCTTGGTCTCTTATGAGGATTTGCATCCTTCTGTTGGAGTTGAAGATCGCTTAGTTTCTCCAAGAATACGATCGCCTCCCTTGGTCTCTTATGAGGATTTGCATCCTGCTGTTGGAATTGAAGAGCATGCACTTCCCTCTTCTGAAGTGGATAGTCGTCGGGAGTTACTTGATCATACTGAATTGCGGGCTCCTCAAGAACCTTCTCTAGTATCACCTTTTGATGGGTCTTATGGTACTGGTAGAAATTTTCCTCTGAAACATGACAATGGTCAAGTCCCGAAAAGAACAAGGCCTTCTGTATCTCCTGTTGGGTTCAATAGTAGATCAAATACCAGTTTTAGTACTCCTGATTCTCGGGTACACAAAAAGCCTTTGCAATCTGCAAGCAATACTATTTCTGAAGCTGCTGCAAGAAATTTGACCAGCGTCCTGGTGGCCAAAAGAACAAGGTCACCTCCTTTACTTCCTGAGGATCAAGTGTTTCACGGAAACTCTTATGCCACTCAAGATGGTACTGAACGAGAAATGCAAGCCAAAGCTAAGCGGTTAGCCCGGTTCAAGGTTGAATTAAGTAAAACTCCACAAAACAATCCTGATATTGTAGAGCCAGGGGTTTCTGCAAATCGACATGAGCAATCTAATGTTGAGGGGAACAAATTAGCTGCATATAAATCTACCAAACTGGCTACGGATGGTACTGATGGCAATGCTTTACCTGAGGATGAAGGCGTGGAGTCGTCTGGTATCATTATTGGGTTGTGCCCGGATATGTGTCCTGAGTCAGAAAGGGCAGAACGAGAAAGGAAAGGGGATCTTGATCAATATGAGCGCTTGGATGGGGATAGAAATCAAACCAGCATGTCCCTTGCTGTTAAAAAGTATAACAGAACAGCTGAGCGAGATGCAAACCTGATACGACCAATGCCAATCCTGCAGAGGACAATTGATTATTTGCTCAATTTGCTTGATAAGCCATACAATGATAGGTTTCTTAGCATATACAACTTCCTATGGGATCGAATGCGAGCAATTCGAATGGACTTGAGAATGCAGCACATTTTTAACCAAGAGGCTATTACTATGCTGGAGCAAATGATAAGACTTCACATAATTGTCATGCATGAATTATGTGAATACTCAAGAGGAGAGGGCTTTTCTGAGGGATTTGATGCACACCTCAATATCGAACAGATGAATAAAACATCTGTTGAATTGTTCCAATTGTATGATGATCACAGAAAGAAAGGAATAAACATTCCTATGGAAAAAGAGTTTCGAGGTTACTATGCCCTTCTCAAGCTGGACAAGCATCCTGGGTACATGGTTGAACCTGCAGAGCTCTCACTTGATCTTGCAAAGATGACTCCAGAAATAAGACAAACTTCAGAGGTGCTATTTGCGCGTGATGTAGCCAGAGCTTGCAGAACTGGTAATTTTATAGCTTTCTTTCGACTTGCGAGGAAAGCAAGTTACCTCCAAGCTTGCCTAATGCATGCACACTTCTCAAAGTTACGAACGCAGGCACTTGCTTCTATACATGCAGGTCTCCAGAATAACCAAGGTCTCCCTATTGCAGATGCTGCCAAGTGGCTGGCAATGGAGGAAGATGAAATTGAAAGCCTTTCAGAGTATCACGGGTTTGCAATAAAGTCATTCCAAGAGCCATACATTGTGAAGGAAGGCCCATTTCTCAATGGTGATGAGGATTATCCTACCAAGTGTTCTAAACTGGTCGATATGAAAAAGTCAAGAAGGATAGTCGAGGATGTTTTGGGTTCTAGTCAAGTCATATCCTTGTCTTCTGAAGCAACAAAAGAAATTCTGATGACTAAGACAAAGAAGCCTGAACCAAAAAATATTTCATATGCTGAAAAGGAAAGTCCCGCTCGTCATGTCCCAGCCATTGAAGTAACAAAATCTGTTAGTGAAGTTGATGAAAAAGGGCCTAATTTTGAAGTTGTTTCATCCCCAAGAGATATCAGACAAAAGAAGCAAATGATTCAAGCACCAATTTTTTCATCTCCAGAAGATGTCAGACAAAAGCAGCAAACGATTCAAATACCAATTTTTCGTCAATACAGTGAAGGTCCCCGTAGGGCATCTGCTGTGTCTCCTTCCCTGCGGGCTTTCTCATCATTTACACCCCAGCCTGATAAAGCTGATACCATGAAGAAGCCAAACTATGATGCTCTTTTTAGTAACTCTCCAGAGGGACTCATGCATTCTGGAATGGAACAAATGCCATTGCAAATTGAGTCAAAAAAAGCTGTACAAGAGAGATCATCTGTTGGTACATATAGTTCCCGTTTGGAATATCCAGTTTTCCAAAATATGGTCACCGATAAGTTGGAAGACGAGGAACCTCCAGATCTCCATCAGGTAGATGAAAACAATGATGTTATGGAGAATTCTCAACAGGAAGAAATTGAGGAGGCAAAACTCAAATTGATACTAAGATTATGGAAGCGACGTTCTCTGAAGCTAAGAGCACTGCGAGAGAAAAAGCAGTTAGCAGCAAATGCTGCATTGAATTCTTTATCATTGGGACCACCAATTCAACTCAAGACAGATCAACCAAACACTTCTGGCGAGTTTGACATTGATCTCGTTTTGAGGGAGAGATATAAAAAGCATGGTCAGTCATGGTCAAGACTTAATGTTTCAGATGTAATAGCGGATATACTAGGCAGAAGAAACCTGGATGCTAGATGCCTATGCTGGAAAACTGTTGTATGTTCTCAGATGAACAATCTAGAAGGTGATGAACTGGGGCAGAGGAGCCATGTCTCAGGAGCAGCCCCCTGGTTGCTTTCAAAGCTCATGCCTTCGGCAAATGATGTGGACGATGATGATGAGGATCTTGTAATTTCATCCCCTGGTGTCTCAATATGGAAGAAATGGGTTCGTGACCAATCTGGTTCAGATCTGAACTGCTGTTTGTCTGTGGTCAAGGATGCAAGTTCTGATAATCCAAGTGAAAGTGTATCCGGTGCAAGTGCTATTTTGTTTCTTACATCTGAAAGCATCCCTTGGAAATTGCAAAAAGTCCATCTTCATAACCTTCTGATGTCAATACCTTATGGTTCCTGCTTGCCCCTTCTGATCTTAAGTGGCTCATACAAGAAAAATGTTGATGACTCCACTATTGTTGATAATTTGGGTCTCCATGACTTGGATAAGTCAAGAATAAGCAGCTTTCATGTTGTTCCGCTTGTAGAAAACCAGCAAATGGGACAATGGGATGGGTTTTTCAGTGACAACCGACTTAGGGAAGGGCTACGATGGCTTGCAAGTGAATCGCCCCTTCAACCCATTCTTCATCATGTGAAAACACGCGAACTCATTCTGACCTACTTGAATTCTTCATTGGACCCATTAGACAAAATGAAAGATTATGAAGTAGGTCCAGACCACTGTATCCTTGCCTTCAATGAAGCCTTGGATTGGTCACAGAAGAAAATTGCTGCTGCTGTGGAATCAAATCCATCCAGTTGGCCTTGCCCTGAGATTGCTTTGCTGGAGGAGTTCAGTGATGAGTACAGGTTTGTCAAGTGGTGCCTGCCAATTGTAGGATGGAGCTCATCGGAAAAAGTTGAACCGCTAATGTCGGCTCTAGGGGATTGTAGACTTCCAACGTTTCCCGACAGTATTTCGTGGCTGCCCAGATGTAATGCAGGAAATGAGATTGGAAACCTGAGAGATGAACTTGAAAATGGCTTGATTGAATACCTGACGCATTCAAGTACGATGATGGGACCTGCACTTGCTATAAAAGAGGCACATGTTATGCTACAAAGAAGCTGCCGACTTGAGCGCCAGGACTCGTGCTGCTATATAGTTCCGAATTGGGTTATGATCTTCCGACGAATTTTCAACTGGCGGTTAATGGGTTTAGCCAATGGGGCCTTCTCTTCAGCCTATGTCTTGGAGAGTCCTCATCTTAACACAGTTTTTGGAAACCTGGGTAAGTTGGGGCTTGAAGATACCGGACCTTCGCCCTGTTACCTTAATCAGCCGACTCTGGATGAAGTCATCGAAGTTGGGTGCGGCCCTCTTTCATCCCATAGAGGGCGGCCCTTGCTAGAATCTGGTCCAGCTCTTCCTGAAACATCTCCTGATGGTGAAATCCACGAGACCCCGAATGCAAATGACTGGATGGAGGATGAACAAAGTTTAGCGCACGATGGTGAAGCGGAAATAGAAAACGTATCCCACGAAAACGGGAGGTTGGAGAATGCTGGCAGAGAAATGGTGGTGACTGGAGAAGTGACCAAGGGAGCTGAAAATTTAAGCATATTGCTAGAACAGTGTAAAATGTTACAGAATGTGATAGACGAGAAGCTGTCCATTTATTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008150 GO:0010468 GO:0019219 GO:0019222 GO:0031323 GO:0031974 GO:0031981 GO:0034399 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044030 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0048583 GO:0050789 GO:0050794 GO:0051052 GO:0051171 GO:0051252 GO:0060147 GO:0060255 GO:0060966 GO:0060968 GO:0065007 GO:0070013 GO:0070920 GO:0080090 GO:0090065 GO:1900368

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM3AP_GANP SAC3_GANP

Protein Analysis

1544

Amino Acids

173.61

Weight (kDa)

5.6

Isoelectric Point (pI)

54.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SAC3_GANP	PF03399	348 - 641	6.4e-82	SAC3/GANP family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007937)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G06290 AT3G06290 AT3G06290 AT3G06290
fragaria_vesca	FvH4_4g13920 FvH4_4g13920 FvH4_4g13920 FvH4_4g13920 FvH4_4g13920
malus_domestica	MD13G1178600.v1.1 MD16G1179800.v1.1
prunus_persica	Prupe.1G147400_v2.0.a1
pyrus_communis	pycom13g15420 pycom16g15090
rosa_chinensis	RchiOBHm_Chr4g0419781
rosa_laevigata	RLG00000007798
rosa_multiflora	Rmu_sc0016868.1_g000004
rosa_roxburghii	Rroxscaffold_5G00362580
rosa_rugosa	Rorug04G0160400
rosa_samantha	Rh4AG221500 Rh4BG223500 Rh4CG234700 Rh4DG220600
rosa_wichuraiana	Rw4G019060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	1612, 1805, 4039
AccB1I	GGYRCC	1 cut(s)	3828
AccB7I	CCANNNNNTGG	3 cut(s)	2605, 3445, 3502
AccBSI	CCGCTC	1 cut(s)	2159
AccI	GTMKAC	1 cut(s)	3898
AccII	CGCG	2 cut(s)	1675, 3609
AclI	AACGTT	1 cut(s)	3908
AclWI	GGATC	4 cut(s)	753, 1092, 1296, 3138
AcoI	YGGCCR	1 cut(s)	708
AcsI	RAATTY	9 cut(s)	214, 517, 688, 2091, 2680, 2803, 3631, 4140, 4558
AcuI	CTGAAG	7 cut(s)	186, 435, 693, 1641, 2097, 2771, 4170
AfeI	AGCGCT	1 cut(s)	1103
AflII	CTTAAG	1 cut(s)	3379
AflIII	ACRYGT	1 cut(s)	4054
AhlI	ACTAGT	1 cut(s)	128
AjnI	CCWGG	9 cut(s)	69, 702, 880, 1585, 2970, 3074, 3148, 4089, 4233
AjuI	GAANNNNNNNTTGG	6 cut(s)	2116, 2148, 3623, 3655, 4163, 4195
AleI	CACNNNNGTG	1 cut(s)	4458
AloI	GAACNNNNNNTCC	2 cut(s)	2728, 2760
Alw21I	GWGCWC	3 cut(s)	1614, 2764, 3854
Alw26I	GTCTC	9 cut(s)	283, 361, 1823, 1841, 2388, 3064, 3160, 3440, 4400
AlwI	GGATC	4 cut(s)	753, 1092, 1296, 3138
AlwNI	CAGNNNCTG	1 cut(s)	1853
Ama87I	CYCGRG	1 cut(s)	627
Aor51HI	AGCGCT	1 cut(s)	1103
AoxI	GGCC	8 cut(s)	563, 708, 1954, 2216, 3767, 4176, 4321, 4346
ApoI	RAATTY	9 cut(s)	214, 517, 688, 2091, 2680, 2803, 3631, 4140, 4558
Asp700I	GAANNNNTTC	1 cut(s)	3615
AspLEI	GCGC	4 cut(s)	1104, 1675, 4089, 4453
AsuC2I	CCSGG	2 cut(s)	826, 1037
AsuHPI	GGTGA	8 cut(s)	477, 717, 1982, 2602, 3044, 4406, 4472, 4540
AsuII	TTCGAA	1 cut(s)	1306
AvaI	CYCGRG	1 cut(s)	627
AvaII	GGWCC	8 cut(s)	6, 2360, 2819, 3643, 3677, 4028, 4262, 4364
AxyI	CCTNAGG	2 cut(s)	741, 990
BaeGI	GKGCMC	1 cut(s)	1037
BaeI	ACNNNNGTAYC	2 cut(s)	3673, 3706
BalI	TGGCCA	1 cut(s)	710
BanI	GGYRCC	1 cut(s)	3828
BanII	GRGCYC	3 cut(s)	463, 1614, 3854
BauI	CACGAG	2 cut(s)	4096, 4403
BbsI	GAAGAC	2 cut(s)	2064, 2631
Bbv12I	GWGCWC	3 cut(s)	1614, 2764, 3854
BceAI	ACGGC	1 cut(s)	77
BcgI	CGANNNNNNTGC	2 cut(s)	3552, 3586
BciT130I	CCWGG	9 cut(s)	71, 704, 882, 1587, 2972, 3076, 3150, 4091, 4235
BciVI	GTATCC	3 cut(s)	3268, 3701, 4492
BclI	TGATCA	3 cut(s)	442, 1090, 1504
BcnI	CCSGG	2 cut(s)	826, 1037
BcoDI	GTCTC	9 cut(s)	283, 361, 1823, 1841, 2388, 3064, 3160, 3440, 4400
BcuI	ACTAGT	1 cut(s)	128
BfmI	CTRYAG	2 cut(s)	1205, 1605
BfoI	RGCGCY	2 cut(s)	1105, 4090
BfrI	CTTAAG	1 cut(s)	3379
BfuAI	ACCTGC	3 cut(s)	1612, 1805, 4039
BfuI	GTATCC	3 cut(s)	3268, 3701, 4492
BglI	GCCNNNNNGGC	1 cut(s)	4175
BglII	AGATCT	2 cut(s)	2641, 3196
BlpI	GCTNAGC	2 cut(s)	813, 1167
BmcAI	AGTACT	1 cut(s)	616
Bme18I	GGWCC	8 cut(s)	6, 2360, 2819, 3643, 3677, 4028, 4262, 4364
BmeT110I	CYCGRG	1 cut(s)	627
BmrI	ACTGGG	1 cut(s)	3050
BmuI	ACTGGG	1 cut(s)	3050
BoxI	GACNNNNGTC	2 cut(s)	540, 698
BpiI	GAAGAC	2 cut(s)	2064, 2631
BpmI	CTGGAG	8 cut(s)	1379, 1623, 1805, 2280, 2463, 2622, 3812, 4554
Bpu1102I	GCTNAGC	2 cut(s)	813, 1167
Bpu14I	TTCGAA	1 cut(s)	1306
BpuEI	CTTGAG	8 cut(s)	207, 450, 762, 1336, 1396, 1553, 2819, 4103
BpuMI	CCSGG	2 cut(s)	826, 1037
BsaBI	GATNNNNATC	7 cut(s)	18, 294, 372, 1089, 1119, 2646, 3129
BsaI	GGTCTC	6 cut(s)	283, 361, 1823, 1841, 3440, 4400
BsaWI	WCCGGW	2 cut(s)	3260, 4259
Bse21I	CCTNAGG	2 cut(s)	741, 990
Bse3DI	GCAATG	3 cut(s)	985, 1875, 2516
Bse8I	GATNNNNATC	7 cut(s)	18, 294, 372, 1089, 1119, 2646, 3129
BseBI	CCWGG	9 cut(s)	71, 704, 882, 1587, 2972, 3076, 3150, 4091, 4235
BseJI	GATNNNNATC	7 cut(s)	18, 294, 372, 1089, 1119, 2646, 3129
BseMI	GCAATG	3 cut(s)	985, 1875, 2516
BseMII	CTCAG	9 cut(s)	732, 981, 1041, 1158, 1422, 3026, 3075, 3768, 3959
BseRI	GAGGAG	5 cut(s)	453, 1431, 2717, 3064, 3809
BseSI	GKGCMC	1 cut(s)	1037
BseYI	CCCAGC	2 cut(s)	2170, 2418
BsgI	GTGCAG	1 cut(s)	4017
Bsh1236I	CGCG	2 cut(s)	1675, 3609
Bsh1285I	CGRYCG	2 cut(s)	266, 344
BshFI	GGCC	8 cut(s)	565, 710, 1956, 2218, 3769, 4178, 4323, 4348
BshNI	GGYRCC	1 cut(s)	3828
BsiEI	CGRYCG	2 cut(s)	266, 344
BsiHKAI	GWGCWC	3 cut(s)	1614, 2764, 3854
BsiHKCI	CYCGRG	1 cut(s)	627
BsiSI	CCGG	4 cut(s)	826, 1037, 3261, 4260
BsmAI	GTCTC	9 cut(s)	283, 361, 1823, 1841, 2388, 3064, 3160, 3440, 4400
BsmI	GAATGC	6 cut(s)	1299, 1326, 2494, 4009, 4420, 4513
BsnI	GGCC	8 cut(s)	565, 710, 1956, 2218, 3769, 4178, 4323, 4348
Bso31I	GGTCTC	6 cut(s)	283, 361, 1823, 1841, 3440, 4400
BsoBI	CYCGRG	1 cut(s)	627
Bsp119I	TTCGAA	1 cut(s)	1306
Bsp1286I	GDGCHC	5 cut(s)	463, 1037, 1614, 2764, 3854
Bsp1407I	TGTACA	1 cut(s)	2542
Bsp1720I	GCTNAGC	2 cut(s)	813, 1167
BspANI	GGCC	8 cut(s)	565, 710, 1956, 2218, 3769, 4178, 4323, 4348
BspCNI	CTCAG	9 cut(s)	733, 982, 1042, 1159, 1423, 3025, 3074, 3769, 3960
BspFNI	CGCG	2 cut(s)	1675, 3609
BspMAI	CTGCAG	2 cut(s)	1209, 1609
BspMI	ACCTGC	3 cut(s)	1612, 1805, 4039
BspPI	GGATC	4 cut(s)	753, 1092, 1296, 3138
BspQI	GCTCTTC	2 cut(s)	387, 4377
BspT104I	TTCGAA	1 cut(s)	1306
BspT107I	GGYRCC	1 cut(s)	3828
BspTI	CTTAAG	1 cut(s)	3379
BspTNI	GGTCTC	6 cut(s)	283, 361, 1823, 1841, 3440, 4400
BsrBI	CCGCTC	1 cut(s)	2159
BsrDI	GCAATG	3 cut(s)	985, 1875, 2516
BsrGI	TGTACA	1 cut(s)	2542
BssSI	CACGAG	2 cut(s)	4096, 4403
BssT1I	CCWWGG	7 cut(s)	141, 273, 351, 1831, 3302, 3711, 4545
Bst2BI	CACGAG	2 cut(s)	4096, 4403
Bst2UI	CCWGG	9 cut(s)	71, 704, 882, 1587, 2972, 3076, 3150, 4091, 4235
Bst4CI	ACNGT	6 cut(s)	2354, 3001, 3689, 3920, 4222, 4581
Bst6I	CTCTTC	5 cut(s)	162, 387, 415, 4189, 4377
BstAFI	CTTAAG	1 cut(s)	3379
BstAPI	GCANNNNNTGC	3 cut(s)	1853, 2792, 2795
BstAUI	TGTACA	1 cut(s)	2542
BstBI	TTCGAA	1 cut(s)	1306
BstEII	GGTNACC	2 cut(s)	723, 2608
BstFNI	CGCG	2 cut(s)	1675, 3609
BstH2I	RGCGCY	2 cut(s)	1105, 4090
BstHHI	GCGC	4 cut(s)	1104, 1675, 4089, 4453
BstMAI	GTCTC	9 cut(s)	283, 361, 1823, 1841, 2388, 3064, 3160, 3440, 4400
BstMCI	CGRYCG	2 cut(s)	266, 344
BstNI	CCWGG	9 cut(s)	71, 704, 882, 1587, 2972, 3076, 3150, 4091, 4235
BstNSI	RCATGY	5 cut(s)	401, 1135, 1769, 1814, 4058
BstPAI	GACNNNNGTC	2 cut(s)	540, 698
BstPI	GGTNACC	2 cut(s)	723, 2608
BstSFI	CTRYAG	2 cut(s)	1205, 1605
BstSLI	GKGCMC	1 cut(s)	1037
BstUI	CGCG	2 cut(s)	1675, 3609
BstV2I	GAAGAC	2 cut(s)	2064, 2631
BstX2I	RGATCY	4 cut(s)	1084, 2641, 3130, 3196
BstXI	CCANNNNNNTGG	3 cut(s)	703, 1864, 3765
BstYI	RGATCY	4 cut(s)	1084, 2641, 3130, 3196
Bsu36I	CCTNAGG	2 cut(s)	741, 990
BsuI	GTATCC	3 cut(s)	3268, 3701, 4492
BsuRI	GGCC	8 cut(s)	565, 710, 1956, 2218, 3769, 4178, 4323, 4348
BtsI	GCAGTG	1 cut(s)	2762
BtsIMutI	CAGTG	7 cut(s)	162, 2359, 2762, 3532, 3685, 3808, 4586
BveI	ACCTGC	3 cut(s)	1612, 1805, 4039
CaiI	CAGNNNCTG	1 cut(s)	1853
CfoI	GCGC	4 cut(s)	1104, 1675, 4089, 4453
CseI	GACGC	2 cut(s)	688, 4015
CspCI	CAANNNNNGTGG	4 cut(s)	3093, 3128, 3403, 3438
EaeI	YGGCCR	1 cut(s)	708
Eam1104I	CTCTTC	5 cut(s)	162, 387, 415, 4189, 4377
EarI	CTCTTC	5 cut(s)	162, 387, 415, 4189, 4377
Ecl136II	GAGCTC	2 cut(s)	1612, 3852
Eco130I	CCWWGG	7 cut(s)	141, 273, 351, 1831, 3302, 3711, 4545
Eco147I	AGGCCT	1 cut(s)	565
Eco24I	GRGCYC	3 cut(s)	463, 1614, 3854
Eco31I	GGTCTC	6 cut(s)	283, 361, 1823, 1841, 3440, 4400
Eco32I	GATATC	1 cut(s)	2251
Eco47I	GGWCC	8 cut(s)	6, 2360, 2819, 3643, 3677, 4028, 4262, 4364
Eco47III	AGCGCT	1 cut(s)	1103
Eco53kI	GAGCTC	2 cut(s)	1612, 3852
Eco57I	CTGAAG	7 cut(s)	186, 435, 693, 1641, 2097, 2771, 4170
Eco81I	CCTNAGG	2 cut(s)	741, 990
Eco88I	CYCGRG	1 cut(s)	627
Eco91I	GGTNACC	2 cut(s)	723, 2608
EcoICRI	GAGCTC	2 cut(s)	1612, 3852
EcoO109I	RGGNCCY	4 cut(s)	2216, 2360, 4028, 4176
EcoO65I	GGTNACC	2 cut(s)	723, 2608
EcoRI	GAATTC	3 cut(s)	2680, 2803, 3631
EcoRII	CCWGG	9 cut(s)	69, 702, 880, 1585, 2970, 3074, 3148, 4089, 4233
EcoRV	GATATC	1 cut(s)	2251
EcoT14I	CCWWGG	7 cut(s)	141, 273, 351, 1831, 3302, 3711, 4545
EcoT22I	ATGCAT	3 cut(s)	1395, 1767, 2496
EcoT38I	GRGCYC	3 cut(s)	463, 1614, 3854
ErhI	CCWWGG	7 cut(s)	141, 273, 351, 1831, 3302, 3711, 4545
FalI	AAGNNNNNCTT	8 cut(s)	1071, 1103, 1639, 1671, 3286, 3318, 3309, 3341
FauI	CCCGC	3 cut(s)	450, 2164, 2388
FauNDI	CATATG	1 cut(s)	2137
FbaI	TGATCA	3 cut(s)	442, 1090, 1504
FblI	GTMKAC	1 cut(s)	3898
FriOI	GRGCYC	3 cut(s)	463, 1614, 3854
GlaI	GCGC	4 cut(s)	1103, 1674, 4088, 4452
GsaI	CCCAGC	2 cut(s)	2174, 2422
GsuI	CTGGAG	8 cut(s)	1379, 1623, 1805, 2280, 2463, 2622, 3812, 4554
HaeII	RGCGCY	2 cut(s)	1105, 4090
HaeIII	GGCC	8 cut(s)	565, 710, 1956, 2218, 3769, 4178, 4323, 4348
HapII	CCGG	4 cut(s)	826, 1037, 3261, 4260
HgaI	GACGC	2 cut(s)	688, 4015
HhaI	GCGC	4 cut(s)	1104, 1675, 4089, 4453
Hin6I	GCGC	4 cut(s)	1102, 1673, 4087, 4451
HinP1I	GCGC	4 cut(s)	1102, 1673, 4087, 4451
HindIII	AAGCTT	1 cut(s)	1752
HpaII	CCGG	4 cut(s)	826, 1037, 3261, 4260
HphI	GGTGA	8 cut(s)	477, 717, 1982, 2602, 3044, 4406, 4472, 4540
Hpy166II	GTNNAC	4 cut(s)	9, 634, 3115, 3899
Hpy8I	GTNNAC	4 cut(s)	9, 634, 3115, 3899
Hpy99I	CGWCG	3 cut(s)	432, 2745, 4140
HpyCH4III	ACNGT	6 cut(s)	2354, 3001, 3689, 3920, 4222, 4581
HpyCH4IV	ACGT	3 cut(s)	2743, 3908, 4479
HpySE526I	ACGT	3 cut(s)	2743, 3908, 4479
HspAI	GCGC	4 cut(s)	1102, 1673, 4087, 4451
Ksp22I	TGATCA	3 cut(s)	442, 1090, 1504
LguI	GCTCTTC	2 cut(s)	387, 4377
LmnI	GCTCC	6 cut(s)	466, 1360, 3051, 3065, 3849, 4550
MaeII	ACGT	3 cut(s)	2743, 3908, 4479
MbiI	CCGCTC	1 cut(s)	2159
MfeI	CAATTG	3 cut(s)	1215, 1493, 3837
MflI	RGATCY	4 cut(s)	1084, 2641, 3130, 3196
MhlI	GDGCHC	5 cut(s)	463, 1037, 1614, 2764, 3854
MlsI	TGGCCA	1 cut(s)	710
MluNI	TGGCCA	1 cut(s)	710
MlyI	GAGTC	9 cut(s)	1016, 1061, 1630, 2481, 2537, 3404, 4088, 4213, 4285
MmeI	TCCRAC	6 cut(s)	286, 364, 2601, 3929, 4159, 4482
Mox20I	TGGCCA	1 cut(s)	710
Mph1103I	ATGCAT	3 cut(s)	1395, 1767, 2496
MroXI	GAANNNNTTC	1 cut(s)	3615
MscI	TGGCCA	1 cut(s)	710
MslI	CAYNNNNRTG	3 cut(s)	537, 1532, 4458
Msp20I	TGGCCA	1 cut(s)	710
MspA1I	CMGCKG	1 cut(s)	1166
MspCI	CTTAAG	1 cut(s)	3379
MspI	CCGG	4 cut(s)	826, 1037, 3261, 4260
MunI	CAATTG	3 cut(s)	1215, 1493, 3837
Mva1269I	GAATGC	6 cut(s)	1299, 1326, 2494, 4009, 4420, 4513
MvaI	CCWGG	9 cut(s)	71, 704, 882, 1587, 2972, 3076, 3150, 4091, 4235
MvnI	CGCG	2 cut(s)	1675, 3609
NciI	CCSGG	2 cut(s)	826, 1037
NdeI	CATATG	1 cut(s)	2137
NmuCI	GTSAC	9 cut(s)	94, 239, 723, 2608, 3179, 3527, 3720, 4528, 4540
NsiI	ATGCAT	3 cut(s)	1395, 1767, 2496
NspI	RCATGY	5 cut(s)	401, 1135, 1769, 1814, 4058
NspV	TTCGAA	1 cut(s)	1306
OliI	CACNNNNGTG	1 cut(s)	4458
PaeI	GCATGC	2 cut(s)	401, 1769
PceI	AGGCCT	1 cut(s)	565
PciI	ACATGT	1 cut(s)	4054
PciSI	GCTCTTC	2 cut(s)	387, 4377
PcsI	WCGNNNNNNNCGW	1 cut(s)	4485
PctI	GAATGC	6 cut(s)	1299, 1326, 2494, 4009, 4420, 4513
PdmI	GAANNNNTTC	1 cut(s)	3615
PfeI	GAWTC	8 cut(s)	14, 153, 623, 2272, 2326, 3569, 3749, 4358
PflMI	CCANNNNNTGG	3 cut(s)	2605, 3445, 3502
Ple19I	CGATCG	2 cut(s)	266, 344
PleI	GAGTC	9 cut(s)	1015, 1060, 1630, 2481, 2536, 3404, 4088, 4212, 4285
PpsI	GAGTC	9 cut(s)	1015, 1060, 1630, 2481, 2536, 3404, 4088, 4212, 4285
PpuMI	RGGWCCY	2 cut(s)	2360, 4028
PscI	ACATGT	1 cut(s)	4054
PshAI	GACNNNNGTC	2 cut(s)	540, 698
Psp124BI	GAGCTC	2 cut(s)	1614, 3854
Psp1406I	AACGTT	1 cut(s)	3908
Psp5II	RGGWCCY	2 cut(s)	2360, 4028
Psp6I	CCWGG	9 cut(s)	69, 702, 880, 1585, 2970, 3074, 3148, 4089, 4233
PspEI	GGTNACC	2 cut(s)	723, 2608
PspFI	CCCAGC	2 cut(s)	2170, 2418
PspGI	CCWGG	9 cut(s)	69, 702, 880, 1585, 2970, 3074, 3148, 4089, 4233
PspPPI	RGGWCCY	2 cut(s)	2360, 4028
PsrI	GAACNNNNNNTAC	4 cut(s)	118, 150, 2557, 2589
PstI	CTGCAG	2 cut(s)	1209, 1609
PstNI	CAGNNNCTG	1 cut(s)	1853
PsuI	RGATCY	4 cut(s)	1084, 2641, 3130, 3196
PvuI	CGATCG	2 cut(s)	266, 344
PvuII	CAGCTG	1 cut(s)	1166
RseI	CAYNNNNRTG	3 cut(s)	537, 1532, 4458
SacI	GAGCTC	2 cut(s)	1614, 3854
SapI	GCTCTTC	2 cut(s)	387, 4377
ScaI	AGTACT	1 cut(s)	616
SchI	GAGTC	9 cut(s)	1016, 1061, 1630, 2481, 2537, 3404, 4088, 4213, 4285
SduI	GDGCHC	5 cut(s)	463, 1037, 1614, 2764, 3854
SfcI	CTRYAG	2 cut(s)	1205, 1605
SfuI	TTCGAA	1 cut(s)	1306
SinI	GGWCC	8 cut(s)	6, 2360, 2819, 3643, 3677, 4028, 4262, 4364
SmiMI	CAYNNNNRTG	3 cut(s)	537, 1532, 4458
SmlI	CTYRAG	9 cut(s)	222, 465, 777, 1315, 1411, 1568, 2834, 3379, 4082
SmoI	CTYRAG	9 cut(s)	222, 465, 777, 1315, 1411, 1568, 2834, 3379, 4082
SpeI	ACTAGT	1 cut(s)	128
SphI	GCATGC	2 cut(s)	401, 1769
SseBI	AGGCCT	1 cut(s)	565
SspI	AATATT	1 cut(s)	2131
SstI	GAGCTC	2 cut(s)	1614, 3854
StuI	AGGCCT	1 cut(s)	565
StyI	CCWWGG	7 cut(s)	141, 273, 351, 1831, 3302, 3711, 4545
TaaI	ACNGT	6 cut(s)	2354, 3001, 3689, 3920, 4222, 4581
TaiI	ACGT	3 cut(s)	2746, 3911, 4482
TaqI	TCGA	9 cut(s)	898, 1291, 1306, 1458, 1549, 1723, 2007, 2034, 4308
TatI	WGTACW	3 cut(s)	614, 2542, 3810
TauI	GCSGC	2 cut(s)	4323, 4348
TfiI	GAWTC	8 cut(s)	14, 153, 623, 2272, 2326, 3569, 3749, 4358
TscAI	CASTG	7 cut(s)	169, 2359, 2769, 3532, 3692, 3808, 4586
TseFI	GTSAC	9 cut(s)	94, 239, 723, 2608, 3179, 3527, 3720, 4528, 4540
Tsp45I	GTSAC	9 cut(s)	94, 239, 723, 2608, 3179, 3527, 3720, 4528, 4540
TspGWI	ACGGA	2 cut(s)	774, 977
TspRI	CASTG	7 cut(s)	169, 2359, 2769, 3532, 3692, 3808, 4586
Van91I	CCANNNNNTGG	3 cut(s)	2605, 3445, 3502
Vha464I	CTTAAG	1 cut(s)	3379
VpaK11BI	GGWCC	8 cut(s)	6, 2360, 2819, 3643, 3677, 4028, 4262, 4364
XapI	RAATTY	9 cut(s)	214, 517, 688, 2091, 2680, 2803, 3631, 4140, 4558
XbaI	TCTAGA	1 cut(s)	3025
XceI	RCATGY	5 cut(s)	401, 1135, 1769, 1814, 4058
XcmI	CCANNNNNNNNNTGG	2 cut(s)	703, 3843
XmiI	GTMKAC	1 cut(s)	3898
XmnI	GAANNNNTTC	1 cut(s)	3615
ZrmI	AGTACT	1 cut(s)	616
Zsp2I	ATGCAT	3 cut(s)	1395, 1767, 2496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.