RchiOBHm_Chr4g0419781

SAC3/GANP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007937
Physical Location & Seq
Reverse (-)
45300431 .. 45311553
11123 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38963

Sequence Viewer

Length: 5028 bp
ATGTTCTCAAGCTTCTCCAGTCAGGCAGGACCCACCGGACCTGCCGGAGAATCACCCATTCCAGTTCAATTCGGCACTTCCGGTCGCCGCCCTCCTTCACCTTCTCCGACTACCCCTCCTTTTCCCTACTCTGCCCCTCTGCGATCTCCTTCCAGTGGACCTGAAGCTCTAGGGCAACCGCATTTGGCATTTCAAAGTTCTCATCCCGGTGGAACTCCTTCGTATCGATTGGCAGGAGTGCAAAGGTCCCCTGAATCTCTTCCTAGTTGGAATGGTGGGCAGAGACCCTTGTTAAAAAATAATGATACCAAGGTTCATCAAAGGCCATCTGCCGTCACATCATTTGTTGTTTCTCGTAATTCTGGAACTAGTGTTACAGCCAAGGTTGCCAGATTCCAAGACACGCGAGGCACCAGATCCCCTCCCTTATTATCAAGAGATGTAAATATAAGAAATTCCACTCAAAGTGTTCCTAGAAGTCACGTAGTTTCTCCAAGAACGCAATCACCTGCCTCGATTTTATATAATTATCAGCCTGTTGAGGATTTCAATCATGTTGGTGCAGTTGAAGGTCACTTAGTTCCTCCAAGAACACGGTCACCTCCCTCGGCATCTTATAACTATCATCCTGTTGAGGATTTGGATCGTTTTGGTGGAGTTGAAGGTCACTTGGTTACTTCAAGAACACAATCACCTCCCTCAGGCTCCTATAACCATCATCCTGTTGAGGACTTTGATCATTCTGGTGGAGTTGAAAGGCATGCATTTTCCTCTTCTGGACTGAATAGTTCGCCGAAGTTGATAGTTGATCATACCAGATTGCAGGCTTATCAAGAACCATCTCGAGTATCACCTTCTGTCAGCACTTTTGACTCTGGTAGAAGAACTCTGGTCAACTATGATGATGTTCAAGTCCTTAAACGAACTAGGCCTTCTGTATCTCCTATTGGGTCAAATGCTACTTCAAGTGCAGTTTTCAGTACTCGTGATTCTCGGGTTCATCAACAGTCTTTGCAGTCTTCAAACAATACTTTTTCTGAAGCTGCTGCAAACAATTTGACCACCATACCGGTTGCCAAAAGAATGAGGTCACCTCCTTTACTTCCTGATGATCAAGTCTTCAAGGGAAACTCATATGCCACTCAAGATGGTACTGAACGAGAAATGCAAGCCAAAGCAAAGCGATTAGCCCGCTTCAAGGTTGAATTAAGTAAAAGTCCACAAAGTGGTATTGATATTGTAGAGCGGGGGGTTTCTGCGACTAGAAATGAACAGTCTAATGTTGAGAGGAACAGATCAGTTGCGTATAGTTCTACTCAATTGGCTAGGGATGTTACTGATGGCAATGCCTTATCTGAGTGTGAAGGTGTAGAATCGTCTGGTATCATTATTGGTGTATGCCCAGATATGTGTCCAGATTCAGAAAGGGCGGAGCGAGAAAGGAAAGGGGATCTTGATCGGCATGAGCGTGTGGATGGGGATAGAAATCAAACCAGCTTGTTCCTTGCTGTTAAAAAGTATAACAGGACAGCTGAGCGGGATGCAAACTTGATAAGACCGATGCCGATCCTGCAGAAGACAATGGATTATTTGCTCAAGTTGCTTGATACACCTTATAGCGATAGGTTTCTTAGCATATATAACTTCTTGTGGGATAGAATGCGGGCGATCCGAATGGACCTAAGAATGCAACACATTTTTAACCAAGAGGCTATTAATATGTTGGAGCAAATGATAAGACTTCACATAATTGCCATGCATGAATTATGTGAATACTCAAAAGGAGAGGGGTTTGCGGAAGGATTTGATGCTCACCTTAATATTGAGCAGATGAATAAAACATCAGTAGAATTGTTCCAAATGTATGATGATCACAGAAAGAAAGGAATAAGCATTCCTACAGAAAAAGAGTTTCGGGGTTATTATGCGCTTCTCAAGCTGGACAAACATCCTGGGCATATGGTTGAACCTGCAGAGCTCTCACTTGATCTTGCAAAGATGACCCCAGAAATAAGACAAACTTCAGAGGTGCTATTTGCACGTGATGTAGCGAGAGCTTGTAGAACTGGTAATTTTATTGCCTTCTTTCGACTTGCAAGGAAAGCAACTTATCTTCAAGCATGCCTAATGCATGCTCATTTTGCAAAGTTAAGAACCCTGGCACTTGCTTCGCTACAAGCAGGGCTCCAGAATAACCAAGGTCTTCCTATTGCGGATGTTGCCAAGTGGCTAGCTATGGAGGAAGAGGAAATTGAAAGCCTTTCAGAGTATCATGGATTCCAAATGAAGTCTTACAATAAAGAGTCATATATGGTAAAGGAAGGTCCATTTCTAAATGGTGATGAGGAATATCCTACCAAGTGTTCTAAACTTGTTGAAATGAAAAAATCAAGAAGGATAATCGAGGATGTTTTAGCTTCTGGTCAAGTCGTATCCTTGCCTGCGGAAGCTTCAAAAGGAATTCAGCTGACTAAACCAAATAAGCTTGGAGCAAAAACTGCTCCATATGGTGAAAGGGGAAGTCTTGTCCATGATGTGCTATCAATAGAAGTAGCAAACTCCATTCATGAAGTTGATGAAGAAATGGCTAATTATGAAGTCGTTTCATCCCCAAAAGATGTCAGACAAAAGCAGAAAATAATTCCAACACCAATTTTTTCACCCCAAAGGGATGTCAGACAAAAGCAGCATATGATTCAAACACCAATAGCTCCGTCCCCAAAGGGTATTAGACAAAAGCAGCAGGTGATTAACATGCCAATTCTTGGTCCACGCCATGAAGATGATAGTCAGATGGCTTTTTTGTCTCCTTCCCCGTGGGATCTCTCATCATATAAACCCCAGCCTGATAAAGTTGGTTTAAATGAGAAGCCCAACAAAGATGCTCCTTACAGTAGCTCTCCAGTGAAAAGCATGCATTTTGGCGTGGAAGCAACGCCATTGCAGATTGGATCAAAAACATCTCCGCAATCTCCAGTTGGTACATATAGTTATGATGCGCAATATTCAGTAGGCCAAATTGTGTCTAATAACTTGGAAAATGAGGAACCTACAGATCTTTGTCAGGACAATGAAAATGATGATGTTATGGGAGATTATCAACATGAGGAAATTGCCGAGGCAAAACTGAAGTTGATGTTAAGGTTATGGAGGCGGCGTTCTATGAAGCTAAGGGAACTGCGAGAGCAAAAGCAGTTGGCAGCAAATGCTGCATTGAGTTCTTTATCACTGGGACCACCAATTCAACTGAAGAGAGATCAACCACGCATGTCTGGTGACTTTGACATTGATCTTATTTTGAGAGAAAGACATCAAAAGCACGGCCAGTCCTGGTCCAGACTTAATGTTTCAGATGTAATAGCAGATACATTAAGCACAAGAAACCCGGATGCTAGATGCCTATGTTGGAAAGTTGTTGTATGTTATCAGATGAACGATGTAGAAGGTGATGAACACTGGCAGAGGAACCATGCCCTGGAAGCAGCACCCTGGTTGCTTTCAAAGATCATGCCTTCAAAAAAAGATCATGAAGATCTCTTAATTTCATCTCCTGGCGTGTCCATATGGAAGAAATGGGTTCAAGGCCAATCTGGTTCAGACCTTACCTGCTGTTTGTCTGTAGTTAAGGATGCAAATAGTGATATTCTAATTGAGTGTGTATCTGGTATGAGTGCGCTATTGTTTCTTGTATCAGAAAGCATCCCTTGGAAATTGCAAAAAGCCCAGCTCCATAACCTTCTGATGTCAGTACCTTATGGTTCCTGCTTACCTCTTCTGATCCTAGCTGGTTCATTCAAGAATGACATTGCAGATCCTTCCTCCATTATAGTTGATAATATGAGTCTCCATGACTTGGATAAGTCCCGAATAAGCAGCTTTCGGATTGTTCCCCTTGTTGAAAACCAGAAAAGAGAGCAATTGGACGGGTTCTATAGTGACAACCGACTGAGGGAGGGGCTCAGATGGCTTGCAAGTGAATCACCTCCTCAACCCATTCTTCATCATGTGAAAACACATGAATTGATTCTGACCCACTGGAATTCCTCATTGGAGGCACTAGAGAAAATGAAAGATTATGAAGTAGGTCCAAACAACTGTATCCTATCCTTCAATGAAGCTTTGGATCAGTCACAGAGGGAAATTGCTGCCACTGCCGAAGCAAATCCTGCTAGTTTCCCTTGCCCTGAAATTGCTTTGCTAGAGGAGTACAGTGAAGAACACAGGCTGGTGAAGTGGTGCTTGCCAAGCATAGGATGGAGCTCAGTAGCAAAAATAGAACCACTAATGTCAGCTCTGGGGAATTGTAGACTTCCCACTTTTCCTAATACTATTTCATGGTTGCCCAGATGTTCATATGCAAGGAAGGAGATTGAGAGCCTGAAAGTTGAACTTGAAAATGGCTTGATCAGATACCTTGCGGATTCTAAGATGATGGGACTTGAACTTGCTATAAAGGAGGCATATGTAATGCTACAAAGAAGTTGTCGACTTGAGTGCCACGACTCATGCTGCTACATTGTGCCCAAATGGACTATGATTTTTCGACGAATCTTCAATTGGCGGTTGATGGGTTTGGCTAATGGGACATTCTCTTCAGCTTATATCCTGGAATGCCCTCATCTCAATGCAACTTTTGGAAACCTGGGTAAGTTGACGCTTGAAGATAGTGAACCTTCAGCTTATCATCTCAATCAGCCGACTCTGGATGAAGTCATTGAAGTTTGCCGCAGCCCTCTAATGTTTCAGAGAGGTCAGTCCTTGCAAGAAACTGATCCGACTCTTCCTGAGACATCACCAAATGGTTCAATTCTTGAGACTCTGAACACAGATGTTTTTATGGATGATGAGACACGCTTAACCGATGATATAGAGGACGTGCCTGACGTAAATAGGGGACTAGAAAATGGTGGCGGAGAACTGGTGGTGGCTGGGAAAGAGACCAAGGAAGATGATAAATTGAGTACATTGTTGGAGCACTGTAACATGTTACAAAATGTGATAGATAAGAAGTTGTCCATTTATTTCTAA

Protein Analysis

1675

Amino Acids

187.67

Weight (kDa)

6.2

Isoelectric Point (pI)

59.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 470 - 763 3.9e-81 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
AarI CACCTGC 2 cut(s) 517, 2734
Acc16I TGCGCA 1 cut(s) 3000
Acc36I ACCTGC 5 cut(s) 49, 517, 1978, 2734, 3614
AccB1I GGYRCC 1 cut(s) 410
AccB7I CCANNNNNTGG 4 cut(s) 1226, 2765, 3475, 3853
AccBSI CCGCTC 2 cut(s) 1246, 1537
AccI GTMKAC 2 cut(s) 4306, 4486
AccII CGCG 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 3322
AcsI RAATTY 3 cut(s) 454, 2460, 4039
AcuI CTGAAG 7 cut(s) 183, 1059, 2007, 3149, 3269, 4578, 4659
AcvI CACGTG 1 cut(s) 2042
AdeI CACNNNGTG 1 cut(s) 1226
AfaI GTAC 6 cut(s) 982, 1153, 2983, 3750, 4208, 4963
AfiI CCNNNNNNNGG 9 cut(s) 155, 701, 1198, 1226, 2765, 3475, 3853, 3949, 4250
AflIII ACRYGT 1 cut(s) 4983
AgeI ACCGGT 1 cut(s) 1069
AhlI ACTAGT 1 cut(s) 368
AjiI CACGTC 1 cut(s) 4876
AjnI CCWGG 8 cut(s) 1951, 2157, 3329, 3474, 3488, 3550, 4605, 4641
AjuI GAANNNNNNNTTGG 4 cut(s) 4031, 4063, 4540, 4572
AloI GAACNNNNNNTCC 2 cut(s) 3142, 3174
Alw21I GWGCWC 3 cut(s) 1980, 4262, 4977
Alw26I GTCTC 7 cut(s) 277, 2811, 3848, 4781, 4808, 4841, 4931
Ama87I CYCGRG 2 cut(s) 843, 993
AoxI GGCC 5 cut(s) 323, 929, 3013, 3322, 3583
ApoI RAATTY 3 cut(s) 454, 2460, 4039
ArsI GACNNNNNNTTYG 2 cut(s) 4468, 4500
AseI ATTAAT 1 cut(s) 1716
AsiGI ACCGGT 1 cut(s) 1069
Asp700I GAANNNNTTC 3 cut(s) 217, 258, 4023
AspLEI GCGC 3 cut(s) 1930, 3001, 3676
AsuC2I CCSGG 2 cut(s) 207, 3386
AsuNHI GCTAGC 1 cut(s) 2230
AvaI CYCGRG 2 cut(s) 843, 993
AxyI CCTNAGG 1 cut(s) 700
BaeGI GKGCMC 1 cut(s) 4524
BaeI ACNNNNGTAYC 4 cut(s) 297, 330, 4081, 4114
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 4 cut(s) 1980, 2187, 3960, 4262
BauI CACGAG 1 cut(s) 984
BbrPI CACGTG 1 cut(s) 2042
BbsI GAAGAC 4 cut(s) 1011, 1111, 1583, 2195
Bbv12I GWGCWC 3 cut(s) 1980, 4262, 4977
BceAI ACGGC 2 cut(s) 317, 3337
BcgI CGANNNNNNTGC 4 cut(s) 2151, 2185, 4476, 4510
BciT130I CCWGG 8 cut(s) 1953, 2159, 3331, 3476, 3490, 3552, 4607, 4643
BciVI GTATCC 2 cut(s) 2443, 4109
BclI TGATCA 5 cut(s) 736, 808, 1111, 1870, 4404
BcnI CCSGG 2 cut(s) 207, 3386
BcoDI GTCTC 7 cut(s) 277, 2811, 3848, 4781, 4808, 4841, 4931
BcuI ACTAGT 1 cut(s) 368
BfmI CTRYAG 6 cut(s) 1571, 1899, 1971, 3051, 3618, 3931
BfuAI ACCTGC 5 cut(s) 49, 517, 1978, 2734, 3614
BfuI GTATCC 2 cut(s) 2443, 4109
BglII AGATCT 2 cut(s) 3055, 3532
BlpI GCTNAGC 1 cut(s) 1533
BmcAI AGTACT 1 cut(s) 982
BmeT110I CYCGRG 2 cut(s) 843, 993
BmgBI CACGTC 1 cut(s) 4876
BmiI GGNNCC 9 cut(s) 31, 248, 412, 706, 2186, 3048, 3234, 3467, 3760
BmrI ACTGGG 1 cut(s) 3239
BmtI GCTAGC 1 cut(s) 2234
BmuI ACTGGG 1 cut(s) 3239
BpiI GAAGAC 4 cut(s) 1011, 1111, 1583, 2195
BplI GAGNNNNNCTC 2 cut(s) 1078, 1110
BpmI CTGGAG 3 cut(s) 2171, 2886, 2958
Bpu10I CCTNAGC 1 cut(s) 3170
Bpu1102I GCTNAGC 1 cut(s) 1533
BpuEI CTTGAG 5 cut(s) 1128, 1580, 1919, 4511, 4832
BpuMI CCSGG 2 cut(s) 207, 3386
Bsa29I ATCGAT 1 cut(s) 226
BsaAI YACGTR 2 cut(s) 484, 2042
BsaBI GATNNNNATC 5 cut(s) 549, 642, 1455, 1485, 1565
BsaI GGTCTC 2 cut(s) 277, 4931
BsaWI WCCGGW 3 cut(s) 35, 80, 1069
BsaXI ACNNNNNCTCC 6 cut(s) 100, 130, 3142, 3172, 4905, 4935
Bsc4I CCNNNNNNNGG 9 cut(s) 155, 701, 1198, 1226, 2765, 3475, 3853, 3949, 4250
Bse118I RCCGGY 1 cut(s) 1069
Bse21I CCTNAGG 1 cut(s) 700
Bse3DI GCAATG 3 cut(s) 1351, 2939, 3804
Bse8I GATNNNNATC 5 cut(s) 549, 642, 1455, 1485, 1565
BseBI CCWGG 8 cut(s) 1953, 2159, 3331, 3476, 3490, 3552, 4607, 4643
BseCI ATCGAT 1 cut(s) 226
BseJI GATNNNNATC 5 cut(s) 549, 642, 1455, 1485, 1565
BseLI CCNNNNNNNGG 9 cut(s) 155, 701, 1198, 1226, 2765, 3475, 3853, 3949, 4250
BseMI GCAATG 3 cut(s) 1351, 2939, 3804
BseMII CTCAG 7 cut(s) 714, 1347, 1524, 3938, 3973, 4275, 4776
BseRI GAGGAG 2 cut(s) 3975, 4217
BseSI GKGCMC 1 cut(s) 4524
BseYI CCCAGC 3 cut(s) 2841, 3723, 4928
BsgI GTGCAG 2 cut(s) 582, 990
Bsh1236I CGCG 1 cut(s) 406
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 5 cut(s) 325, 931, 3015, 3324, 3585
BshNI GGYRCC 1 cut(s) 410
BshTI ACCGGT 1 cut(s) 1069
BshVI ATCGAT 1 cut(s) 226
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 3 cut(s) 1980, 4262, 4977
BsiHKCI CYCGRG 2 cut(s) 843, 993
BsiSI CCGG 6 cut(s) 36, 45, 81, 207, 1070, 3386
BslFI GGGAC 7 cut(s) 232, 2701, 3246, 3847, 4449, 4597, 4908
BslI CCNNNNNNNGG 9 cut(s) 155, 701, 1198, 1226, 2765, 3475, 3853, 3949, 4250
BsmAI GTCTC 7 cut(s) 277, 2811, 3848, 4781, 4808, 4841, 4931
BsmFI GGGAC 7 cut(s) 232, 2701, 3246, 3847, 4449, 4597, 4908
BsmI GAATGC 4 cut(s) 1665, 1692, 1893, 4616
BsnI GGCC 5 cut(s) 325, 931, 3015, 3324, 3585
Bso31I GGTCTC 2 cut(s) 277, 4931
BsoBI CYCGRG 2 cut(s) 843, 993
Bsp1286I GDGCHC 6 cut(s) 1980, 2187, 3960, 4262, 4524, 4977
Bsp1720I GCTNAGC 1 cut(s) 1533
BspANI GGCC 5 cut(s) 325, 931, 3015, 3324, 3585
BspCNI CTCAG 7 cut(s) 713, 1348, 1525, 3939, 3972, 4274, 4777
BspDI ATCGAT 1 cut(s) 226
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 2 cut(s) 2566, 3526
BspLI GGNNCC 9 cut(s) 31, 248, 412, 706, 2186, 3048, 3234, 3467, 3760
BspMAI CTGCAG 2 cut(s) 1575, 1975
BspMI ACCTGC 5 cut(s) 49, 517, 1978, 2734, 3614
BspOI GCTAGC 1 cut(s) 2234
BspT107I GGYRCC 1 cut(s) 410
BspTNI GGTCTC 2 cut(s) 277, 4931
BsrBI CCGCTC 2 cut(s) 1246, 1537
BsrDI GCAATG 3 cut(s) 1351, 2939, 3804
BsrFI RCCGGY 1 cut(s) 1069
BssAI RCCGGY 1 cut(s) 1069
BssSI CACGAG 1 cut(s) 984
BssT1I CCWWGG 5 cut(s) 309, 381, 2197, 3704, 4941
Bst2BI CACGAG 1 cut(s) 984
Bst2UI CCWGG 8 cut(s) 1953, 2159, 3331, 3476, 3490, 3552, 4607, 4643
Bst4CI ACNGT 7 cut(s) 597, 1008, 1275, 2894, 4097, 4211, 4979
Bst6I CTCTTC 7 cut(s) 264, 778, 2238, 3245, 3777, 4597, 4785
BstAPI GCANNNNNTGC 4 cut(s) 2498, 3206, 3209, 4166
BstBAI YACGTR 2 cut(s) 484, 2042
BstDSI CCRYGG 1 cut(s) 2816
BstEII GGTNACC 2 cut(s) 597, 1089
BstENI CCTNNNNNAGG 1 cut(s) 699
BstFNI CGCG 1 cut(s) 406
BstHHI GCGC 3 cut(s) 1930, 3001, 3676
BstMAI GTCTC 7 cut(s) 277, 2811, 3848, 4781, 4808, 4841, 4931
BstMCI CGRYCG 1 cut(s) 85
BstNI CCWGG 8 cut(s) 1953, 2159, 3331, 3476, 3490, 3552, 4607, 4643
BstNSI RCATGY 7 cut(s) 764, 2124, 2135, 2758, 2917, 3271, 4987
BstPI GGTNACC 2 cut(s) 597, 1089
BstSFI CTRYAG 6 cut(s) 1571, 1899, 1971, 3051, 3618, 3931
BstSLI GKGCMC 1 cut(s) 4524
BstUI CGCG 1 cut(s) 406
BstV2I GAAGAC 4 cut(s) 1011, 1111, 1583, 2195
BstX2I RGATCY 6 cut(s) 416, 1450, 2821, 3055, 3532, 3811
BstYI RGATCY 6 cut(s) 416, 1450, 2821, 3055, 3532, 3811
Bsu15I ATCGAT 1 cut(s) 226
Bsu36I CCTNAGG 1 cut(s) 700
BsuI GTATCC 2 cut(s) 2443, 4109
BsuRI GGCC 5 cut(s) 325, 931, 3015, 3324, 3585
BsuTUI ATCGAT 1 cut(s) 226
BtgI CCRYGG 1 cut(s) 2816
BtrI CACGTC 1 cut(s) 4876
BtsI GCAGTG 1 cut(s) 4149
BtsIMutI CAGTG 8 cut(s) 160, 2910, 3227, 3454, 4033, 4149, 4216, 4975
BveI ACCTGC 5 cut(s) 49, 517, 1978, 2734, 3614
CciI TCATGA 2 cut(s) 2566, 3526
CfoI GCGC 3 cut(s) 1930, 3001, 3676
Cfr10I RCCGGY 1 cut(s) 1069
ClaI ATCGAT 1 cut(s) 226
CseI GACGC 1 cut(s) 4663
Csp6I GTAC 6 cut(s) 981, 1152, 2982, 3749, 4207, 4962
CspAI ACCGGT 1 cut(s) 1069
CviQI GTAC 6 cut(s) 981, 1152, 2982, 3749, 4207, 4962
DraI TTTAAA 1 cut(s) 2862
DraIII CACNNNGTG 1 cut(s) 1226
EaeI YGGCCR 1 cut(s) 3322
Eam1104I CTCTTC 7 cut(s) 264, 778, 2238, 3245, 3777, 4597, 4785
EarI CTCTTC 7 cut(s) 264, 778, 2238, 3245, 3777, 4597, 4785
EciI GGCGGA 2 cut(s) 1445, 4926
Ecl136II GAGCTC 2 cut(s) 1978, 4260
Eco130I CCWWGG 5 cut(s) 309, 381, 2197, 3704, 4941
Eco147I AGGCCT 1 cut(s) 931
Eco24I GRGCYC 4 cut(s) 1980, 2187, 3960, 4262
Eco31I GGTCTC 2 cut(s) 277, 4931
Eco53kI GAGCTC 2 cut(s) 1978, 4260
Eco57I CTGAAG 7 cut(s) 183, 1059, 2007, 3149, 3269, 4578, 4659
Eco72I CACGTG 1 cut(s) 2042
Eco81I CCTNAGG 1 cut(s) 700
Eco88I CYCGRG 2 cut(s) 843, 993
Eco91I GGTNACC 2 cut(s) 597, 1089
EcoICRI GAGCTC 2 cut(s) 1978, 4260
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoO109I RGGNCCY 2 cut(s) 29, 246
EcoO65I GGTNACC 2 cut(s) 597, 1089
EcoRI GAATTC 2 cut(s) 2460, 4039
EcoRII CCWGG 8 cut(s) 1951, 2157, 3329, 3474, 3488, 3550, 4605, 4641
EcoT14I CCWWGG 5 cut(s) 309, 381, 2197, 3704, 4941
EcoT22I ATGCAT 4 cut(s) 766, 1761, 2133, 2919
EcoT38I GRGCYC 4 cut(s) 1980, 2187, 3960, 4262
ErhI CCWWGG 5 cut(s) 309, 381, 2197, 3704, 4941
FaqI GGGAC 7 cut(s) 232, 2701, 3246, 3847, 4449, 4597, 4908
FauI CCCGC 4 cut(s) 1199, 1239, 1530, 1656
FauNDI CATATG 7 cut(s) 1135, 1959, 2506, 2691, 3563, 4354, 4462
FbaI TGATCA 5 cut(s) 736, 808, 1111, 1870, 4404
FblI GTMKAC 2 cut(s) 4306, 4486
FriOI GRGCYC 4 cut(s) 1980, 2187, 3960, 4262
FspI TGCGCA 1 cut(s) 3000
GlaI GCGC 3 cut(s) 1929, 3000, 3675
GsaI CCCAGC 3 cut(s) 2845, 3727, 4932
GsuI CTGGAG 3 cut(s) 2171, 2886, 2958
HaeIII GGCC 5 cut(s) 325, 931, 3015, 3324, 3585
HapII CCGG 6 cut(s) 36, 45, 81, 207, 1070, 3386
HgaI GACGC 1 cut(s) 4663
HhaI GCGC 3 cut(s) 1930, 3001, 3676
Hin6I GCGC 3 cut(s) 1928, 2999, 3674
HinP1I GCGC 3 cut(s) 1928, 2999, 3674
HincII GTYRAC 3 cut(s) 895, 4487, 4653
HindII GTYRAC 3 cut(s) 895, 4487, 4653
HindIII AAGCTT 4 cut(s) 10, 2448, 2483, 4116
HpaII CCGG 6 cut(s) 36, 45, 81, 207, 1070, 3386
Hpy166II GTNNAC 8 cut(s) 158, 895, 1220, 2771, 4307, 4487, 4653, 4670
Hpy8I GTNNAC 8 cut(s) 158, 895, 1220, 2771, 4307, 4487, 4653, 4670
Hpy99I CGWCG 1 cut(s) 4548
HpyCH4III ACNGT 7 cut(s) 597, 1008, 1275, 2894, 4097, 4211, 4979
HpyCH4IV ACGT 4 cut(s) 483, 2041, 4875, 4884
HpySE526I ACGT 4 cut(s) 483, 2041, 4875, 4884
HspAI GCGC 3 cut(s) 1928, 2999, 3674
Ksp22I TGATCA 5 cut(s) 736, 808, 1111, 1870, 4404
MaeII ACGT 4 cut(s) 483, 2041, 4875, 4884
MbiI CCGCTC 2 cut(s) 1246, 1537
MfeI CAATTG 3 cut(s) 1319, 3917, 4555
MflI RGATCY 6 cut(s) 416, 1450, 2821, 3055, 3532, 3811
MhlI GDGCHC 6 cut(s) 1980, 2187, 3960, 4262, 4524, 4977
MlyI GAGTC 7 cut(s) 866, 2312, 3850, 4496, 4693, 4771, 4810
MmeI TCCRAC 7 cut(s) 131, 248, 1704, 2669, 3386, 4799, 4950
Mph1103I ATGCAT 4 cut(s) 766, 1761, 2133, 2919
MroXI GAANNNNTTC 3 cut(s) 217, 258, 4023
MslI CAYNNNNRTG 6 cut(s) 207, 558, 744, 1467, 2781, 4623
MspA1I CMGCKG 2 cut(s) 1532, 2467
MspI CCGG 6 cut(s) 36, 45, 81, 207, 1070, 3386
MunI CAATTG 3 cut(s) 1319, 3917, 4555
Mva1269I GAATGC 4 cut(s) 1665, 1692, 1893, 4616
MvaI CCWGG 8 cut(s) 1953, 2159, 3331, 3476, 3490, 3552, 4607, 4643
MvnI CGCG 1 cut(s) 406
NciI CCSGG 2 cut(s) 207, 3386
NdeI CATATG 7 cut(s) 1135, 1959, 2506, 2691, 3563, 4354, 4462
NheI GCTAGC 1 cut(s) 2230
NlaIV GGNNCC 9 cut(s) 31, 248, 412, 706, 2186, 3048, 3234, 3467, 3760
NmeAIII GCCGAG 2 cut(s) 587, 3142
NmuCI GTSAC 9 cut(s) 334, 479, 572, 597, 665, 1089, 3275, 3935, 4128
NsbI TGCGCA 1 cut(s) 3000
NsiI ATGCAT 4 cut(s) 766, 1761, 2133, 2919
NspI RCATGY 7 cut(s) 764, 2124, 2135, 2758, 2917, 3271, 4987
PaeI GCATGC 4 cut(s) 764, 2124, 2135, 2917
PaeR7I CTCGAG 1 cut(s) 843
PagI TCATGA 2 cut(s) 2566, 3526
PaqCI CACCTGC 2 cut(s) 517, 2734
PceI AGGCCT 1 cut(s) 931
PciI ACATGT 1 cut(s) 4983
PcsI WCGNNNNNNNCGW 2 cut(s) 1465, 4881
PctI GAATGC 4 cut(s) 1665, 1692, 1893, 4616
PdmI GAANNNNTTC 3 cut(s) 217, 258, 4023
PflMI CCANNNNNTGG 4 cut(s) 1226, 2765, 3475, 3853
PfoI TCCNGGA 1 cut(s) 4605
PinAI ACCGGT 1 cut(s) 1069
PleI GAGTC 7 cut(s) 866, 2311, 3849, 4496, 4693, 4771, 4810
PmaCI CACGTG 1 cut(s) 2042
PmlI CACGTG 1 cut(s) 2042
PpsI GAGTC 7 cut(s) 866, 2311, 3849, 4496, 4693, 4771, 4810
Ppu21I YACGTR 2 cut(s) 484, 2042
PpuMI RGGWCCY 2 cut(s) 29, 246
PscI ACATGT 1 cut(s) 4983
PshBI ATTAAT 1 cut(s) 1716
PsiI TTATAA 1 cut(s) 618
Psp124BI GAGCTC 2 cut(s) 1980, 4262
Psp5II RGGWCCY 2 cut(s) 29, 246
Psp6I CCWGG 8 cut(s) 1951, 2157, 3329, 3474, 3488, 3550, 4605, 4641
PspCI CACGTG 1 cut(s) 2042
PspEI GGTNACC 2 cut(s) 597, 1089
PspFI CCCAGC 3 cut(s) 2841, 3723, 4928
PspGI CCWGG 8 cut(s) 1951, 2157, 3329, 3474, 3488, 3550, 4605, 4641
PspN4I GGNNCC 9 cut(s) 31, 248, 412, 706, 2186, 3048, 3234, 3467, 3760
PspPPI RGGWCCY 2 cut(s) 29, 246
PsrI GAACNNNNNNTAC 2 cut(s) 358, 390
PstI CTGCAG 2 cut(s) 1575, 1975
PsuI RGATCY 6 cut(s) 416, 1450, 2821, 3055, 3532, 3811
PvuII CAGCTG 2 cut(s) 1532, 2467
RsaI GTAC 6 cut(s) 982, 1153, 2983, 3750, 4208, 4963
RsaNI GTAC 6 cut(s) 981, 1152, 2982, 3749, 4207, 4962
RseI CAYNNNNRTG 6 cut(s) 207, 558, 744, 1467, 2781, 4623
SacI GAGCTC 2 cut(s) 1980, 4262
SalI GTCGAC 1 cut(s) 4485
ScaI AGTACT 1 cut(s) 982
SchI GAGTC 7 cut(s) 866, 2312, 3850, 4496, 4693, 4771, 4810
SduI GDGCHC 6 cut(s) 1980, 2187, 3960, 4262, 4524, 4977
SfcI CTRYAG 6 cut(s) 1571, 1899, 1971, 3051, 3618, 3931
Sfr274I CTCGAG 1 cut(s) 843
SlaI CTCGAG 1 cut(s) 843
SmiMI CAYNNNNRTG 6 cut(s) 207, 558, 744, 1467, 2781, 4623
SmlI CTYRAG 7 cut(s) 7, 843, 1143, 1595, 1934, 4490, 4811
SmoI CTYRAG 7 cut(s) 7, 843, 1143, 1595, 1934, 4490, 4811
SpeI ACTAGT 1 cut(s) 368
SphI GCATGC 4 cut(s) 764, 2124, 2135, 2917
SseBI AGGCCT 1 cut(s) 931
SspI AATATT 2 cut(s) 1822, 3005
SstI GAGCTC 2 cut(s) 1980, 4262
StuI AGGCCT 1 cut(s) 931
StyI CCWWGG 5 cut(s) 309, 381, 2197, 3704, 4941
TaaI ACNGT 7 cut(s) 597, 1008, 1275, 2894, 4097, 4211, 4979
TaiI ACGT 4 cut(s) 486, 2044, 4878, 4887
TaqI TCGA 7 cut(s) 226, 515, 844, 2089, 2403, 4486, 4543
TaqII GACCGA 1 cut(s) 1573
TatI WGTACW 3 cut(s) 980, 4206, 4961
TauI GCSGC 3 cut(s) 90, 3157, 4728
TscAI CASTG 8 cut(s) 160, 2910, 3234, 3461, 4040, 4156, 4216, 4982
TseFI GTSAC 9 cut(s) 334, 479, 572, 597, 665, 1089, 3275, 3935, 4128
Tsp45I GTSAC 9 cut(s) 334, 479, 572, 597, 665, 1089, 3275, 3935, 4128
TspGWI ACGGA 1 cut(s) 2703
TspRI CASTG 8 cut(s) 160, 2910, 3234, 3461, 4040, 4156, 4216, 4982
Van91I CCANNNNNTGG 4 cut(s) 1226, 2765, 3475, 3853
VspI ATTAAT 1 cut(s) 1716
XagI CCTNNNNNAGG 1 cut(s) 699
XapI RAATTY 3 cut(s) 454, 2460, 4039
XceI RCATGY 7 cut(s) 764, 2124, 2135, 2758, 2917, 3271, 4987
XcmI CCANNNNNNNNNTGG 1 cut(s) 4251
XhoI CTCGAG 1 cut(s) 843
XmiI GTMKAC 2 cut(s) 4306, 4486
XmnI GAANNNNTTC 3 cut(s) 217, 258, 4023
ZrmI AGTACT 1 cut(s) 982
Zsp2I ATGCAT 4 cut(s) 766, 1761, 2133, 2919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.