Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0006336

Physical Location & Seq

Genomic Coordinates Reverse (-)

20300870 .. 20305997

Gene Structure 5128 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g16310.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1374 bp

ATGATGGAGATTGACATTCATGAGGAAAGCGATGGAAGCACACCTAGGGCAAATGAAAATGACCTGTCTAATAGGCGAGTTGCACCTGATGAGTCTGGTGAAGGTTTGCCTTATGCTCCTGTTAATTGGCCTAATCCCGGTGATACTTGGCGGTGGAAAGTGGGAAAGCGAGTTTCTGGATCTGGACACTTTCTGGATAGGTACCTTTATCTTCCAAAGAGACTTTGCCATGATGGGGCCGGGAATTCTACGGGTAGGAGAAATAGTTTTGCAAGCAAGCTTGCAGTTGAACGATATATCCAAGCATCATTTCCTGGTACAGACCTCAAAGAATTTTTTGCATCATTCTCGTGGAAGATTCCAGCGGAAATGTCACTGTTAGCAAATGGTGCTGTAAAGCTGAAGCATGTTCCAGCTGTTCCACCTATTCCACCTTGTGTGGGAATTGAAGAGTCTGATTCTCAGTCTGATGGCTTATGCTGTAAGGCCCGAAATAAGTTGTGCATCAGTTTGTTGGAAAAAGCAGAAAACCCACCCTTAGTATTCATGGAGTGTGATATTTGCTGCACTGAGGCTAAGTTCTGTCGAGATTGCTGCTGCATTCTTTGTTCGAAGACTATAAATTTCTCCTATGGAGGCTATGATTACATCAGATGTGAAGCACTGACGGGCGAGGCTGTTATATGTGGGCATGCTGCCCACATTGATTGTGCTCTCCGATGCTACATGGCTGGCACTGTAGGAGGAAGCATTGCATTGGATTCTGAGTACTATTGCCGGCGCTGTGATGCAAGGACAGATCTGGTTCCACATGTGATGAGGCTTTTACACACATGTGAATCCCTTGATTCTCGGGACAACATTGAGAAGATCTTAAATATTGGTATCTGCATCTTGCGCAGTTCCGGGAAAACCATTGCTAAGGAGTTGCTCAAACGTATTGAAGTGGCTATTGCAAAGCTCAAATATGGGACTTCTCTTGAAGATATTTGGAAGCAGGAAGATGGCTCTCATCATGAAAGTACTGCTATGGAAGTGACAACAAACCAGGAGCCCTTGAATGAAGACGTTGATCAGGTTCTGCATGCACTAAAGAACTCCCAAGAGTCTGAATATAAGGTGGTTGAGGAAAAACTTCTTGCACAGAAGAACTATATTTGTAATCTGTATCAGCAGCTTGAGAAGGAGAAGTCTGATCTGGCACGTCGCACATCAACCGACCCTGATGCTTTACTCGATATTGTATTGAGCAGGGTGAAGCAGATTAAAAGGGAAATCACAAATCTCAAGGAGATGGAGCAGATAGCCTCTGGATTTGGAAAGACTTCCAGAGCTACCCTGAAAAAGCACTTCAATTTTGAAGTTGAGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-bind_3 PHD_Oberon

Protein Analysis

458

Amino Acids

51.2

Weight (kDa)

5.84

Isoelectric Point (pI)

46.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7081	PF23299	27 - 122	1.1e-39	Domain of unknown function (DUF7081)
PHD_Oberon	PF07227	163 - 297	5e-34	PHD - plant homeodomain finger protein
DUF7615	PF24590	370 - 472	8.5e-39	Domain of unknown function (DUF7615)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006336)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05410 AT1G05410
fragaria_vesca	FvH4_4g16310 FvH4_4g16310 FvH4_4g16310 FvH4_4g16310 FvH4_4g16310 FvH4_4g16310 FvH4_4g16310 FvH4_4g16310
malus_domestica	MD13G1194400.v1.1
prunus_persica	Prupe.1G015300_v2.0.a1 Prupe.1G015300_v2.0.a1 Prupe.1G015300_v2.0.a1 Prupe.1G015300_v2.0.a1
pyrus_communis	pycom13g16680
rosa_chinensis	RchiOBHm_Chr4g0416001
rosa_laevigata	RLG00000008024
rosa_multiflora	Rmu_sc0005415.1_g000007
rosa_roxburghii	Rroxscaffold_5G00359850
rosa_rugosa	Rorug04G0137300 Rorug04G0137400
rosa_samantha	Rh4AG199800 Rh4BG197300 Rh4CG210900 Rh4DG197500
rosa_wichuraiana	Rw4G016940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	899
Acc65I	GGTACC	1 cut(s)	201
AccB1I	GGYRCC	1 cut(s)	201
AciI	CCGC	2 cut(s)	151, 365
AclWI	GGATC	1 cut(s)	187
AcsI	RAATTY	3 cut(s)	244, 332, 622
AcuI	CTGAAG	1 cut(s)	422
AdeI	CACNNNGTG	1 cut(s)	437
AfaI	GTAC	4 cut(s)	203, 319, 770, 1024
AfiI	CCNNNNNNNGG	3 cut(s)	137, 235, 440
AflIII	ACRYGT	2 cut(s)	811, 833
AgsI	TTSAA	7 cut(s)	290, 449, 944, 983, 1060, 1354, 1361
AjiI	CACGTC	1 cut(s)	1205
AjnI	CCWGG	2 cut(s)	313, 1047
AjuI	GAANNNNNNNTTGG	2 cut(s)	294, 326
AleI	CACNNNNGTG	1 cut(s)	834
AloI	GAACNNNNNNTCC	2 cut(s)	282, 314
AluBI	AGCT	6 cut(s)	280, 400, 416, 961, 1177, 1334
AluI	AGCT	6 cut(s)	280, 400, 416, 961, 1177, 1334
Alw21I	GWGCWC	1 cut(s)	715
Alw26I	GTCTC	1 cut(s)	214
AlwI	GGATC	1 cut(s)	187
AlwNI	CAGNNNCTG	1 cut(s)	1081
Ama87I	CYCGRG	1 cut(s)	852
AoxI	GGCC	3 cut(s)	128, 237, 486
ApeKI	GCWGC	5 cut(s)	564, 594, 597, 695, 1174
ApoI	RAATTY	3 cut(s)	244, 332, 622
Asp700I	GAANNNNTTC	2 cut(s)	1134, 1324
Asp718I	GGTACC	1 cut(s)	201
AspA2I	CCTAGG	1 cut(s)	44
AspLEI	GCGC	2 cut(s)	783, 900
AspS9I	GGNCC	2 cut(s)	237, 487
AsuC2I	CCSGG	3 cut(s)	138, 241, 907
AsuHPI	GGTGA	3 cut(s)	110, 152, 1267
AsuII	TTCGAA	1 cut(s)	611
AvaI	CYCGRG	1 cut(s)	852
AvrII	CCTAGG	1 cut(s)	44
BanI	GGYRCC	1 cut(s)	201
BanII	GRGCYC	1 cut(s)	1056
BauI	CACGAG	1 cut(s)	349
BbsI	GAAGAC	2 cut(s)	620, 1071
Bbv12I	GWGCWC	1 cut(s)	715
BbvI	GCAGC	5 cut(s)	551, 581, 584, 682, 1186
BccI	CCATC	5 cut(s)	26, 227, 464, 998, 1288
BcgI	CGANNNNNNTGC	6 cut(s)	330, 364, 576, 610, 1208, 1242
BciT130I	CCWGG	2 cut(s)	315, 1049
BclI	TGATCA	1 cut(s)	1072
BcnI	CCSGG	3 cut(s)	138, 241, 907
BcoDI	GTCTC	1 cut(s)	214
BfaI	CTAG	1 cut(s)	45
BfmI	CTRYAG	1 cut(s)	738
BfoI	RGCGCY	1 cut(s)	784
BglII	AGATCT	2 cut(s)	799, 870
BisI	GCNGC	5 cut(s)	565, 595, 598, 696, 1175
BlnI	CCTAGG	1 cut(s)	44
BlsI	GCNGC	5 cut(s)	566, 596, 599, 697, 1176
BmcAI	AGTACT	2 cut(s)	770, 1024
Bme1390I	CCNGG	5 cut(s)	138, 241, 315, 907, 1049
BmeT110I	CYCGRG	1 cut(s)	852
BmgBI	CACGTC	1 cut(s)	1205
BmgT120I	GGNCC	2 cut(s)	237, 487
BmiI	GGNNCC	4 cut(s)	203, 238, 807, 1053
BmrFI	CCNGG	5 cut(s)	138, 241, 315, 907, 1049
BmsI	GCATC	7 cut(s)	314, 350, 513, 710, 778, 900, 1216
BpiI	GAAGAC	2 cut(s)	620, 1071
Bpu10I	CCTNAGC	1 cut(s)	921
Bpu14I	TTCGAA	1 cut(s)	611
BpuEI	CTTGAG	2 cut(s)	1199, 1271
BpuMI	CCSGG	3 cut(s)	138, 241, 907
BsaJI	CCNNGG	1 cut(s)	44
BsaXI	ACNNNNNCTCC	2 cut(s)	250, 280
Bsc4I	CCNNNNNNNGG	3 cut(s)	137, 235, 440
Bse118I	RCCGGY	1 cut(s)	777
Bse3DI	GCAATG	2 cut(s)	750, 915
BseBI	CCWGG	2 cut(s)	315, 1049
BseDI	CCNNGG	1 cut(s)	44
BseLI	CCNNNNNNNGG	3 cut(s)	137, 235, 440
BseMI	GCAATG	2 cut(s)	750, 915
BseMII	CTCAG	3 cut(s)	476, 561, 756
BseXI	GCAGC	5 cut(s)	551, 581, 584, 682, 1186
BsgI	GTGCAG	1 cut(s)	550
BshFI	GGCC	3 cut(s)	130, 239, 488
BshNI	GGYRCC	1 cut(s)	201
BsiHKAI	GWGCWC	1 cut(s)	715
BsiHKCI	CYCGRG	1 cut(s)	852
BsiSI	CCGG	4 cut(s)	138, 240, 778, 906
BslFI	GGGAC	2 cut(s)	869, 985
BslI	CCNNNNNNNGG	3 cut(s)	137, 235, 440
BsmAI	GTCTC	1 cut(s)	214
BsmFI	GGGAC	2 cut(s)	869, 985
BsmI	GAATGC	1 cut(s)	600
BsnI	GGCC	3 cut(s)	130, 239, 488
BsoBI	CYCGRG	1 cut(s)	852
Bsp119I	TTCGAA	1 cut(s)	611
Bsp1286I	GDGCHC	2 cut(s)	715, 1056
Bsp143I	GATC	5 cut(s)	179, 799, 870, 1072, 1195
BspACI	CCGC	2 cut(s)	151, 365
BspANI	GGCC	3 cut(s)	130, 239, 488
BspCNI	CTCAG	3 cut(s)	475, 562, 757
BspHI	TCATGA	2 cut(s)	19, 1015
BspLI	GGNNCC	4 cut(s)	203, 238, 807, 1053
BspPI	GGATC	1 cut(s)	187
BspT104I	TTCGAA	1 cut(s)	611
BspT107I	GGYRCC	1 cut(s)	201
BsrDI	GCAATG	2 cut(s)	750, 915
BsrFI	RCCGGY	1 cut(s)	777
BssAI	RCCGGY	1 cut(s)	777
BssECI	CCNNGG	1 cut(s)	44
BssMI	GATC	5 cut(s)	179, 799, 870, 1072, 1195
BssSI	CACGAG	1 cut(s)	349
BssT1I	CCWWGG	1 cut(s)	44
Bst2BI	CACGAG	1 cut(s)	349
Bst2UI	CCWGG	2 cut(s)	315, 1049
Bst4CI	ACNGT	2 cut(s)	378, 739
Bst6I	CTCTTC	1 cut(s)	444
BstAPI	GCANNNNNTGC	1 cut(s)	389
BstBI	TTCGAA	1 cut(s)	611
BstC8I	GCNNGC	7 cut(s)	274, 278, 282, 693, 733, 779, 1086
BstDEI	CTNAG	6 cut(s)	462, 538, 570, 576, 765, 921
BstH2I	RGCGCY	1 cut(s)	784
BstHHI	GCGC	2 cut(s)	783, 900
BstKTI	GATC	5 cut(s)	182, 802, 873, 1075, 1198
BstMAI	GTCTC	1 cut(s)	214
BstMBI	GATC	5 cut(s)	179, 799, 870, 1072, 1195
BstMWI	GCNNNNNNNGC	3 cut(s)	36, 389, 897
BstNI	CCWGG	2 cut(s)	315, 1049
BstNSI	RCATGY	5 cut(s)	410, 695, 815, 837, 1088
BstSCI	CCNGG	5 cut(s)	136, 239, 313, 905, 1047
BstSFI	CTRYAG	1 cut(s)	738
BstV1I	GCAGC	5 cut(s)	551, 581, 584, 682, 1186
BstV2I	GAAGAC	2 cut(s)	620, 1071
BstX2I	RGATCY	3 cut(s)	179, 799, 870
BstYI	RGATCY	3 cut(s)	179, 799, 870
BsuRI	GGCC	3 cut(s)	130, 239, 488
BtgZI	GCGATG	1 cut(s)	45
BtrI	CACGTC	1 cut(s)	1205
BtsIMutI	CAGTG	4 cut(s)	374, 567, 662, 735
Cac8I	GCNNGC	7 cut(s)	274, 278, 282, 693, 733, 779, 1086
CaiI	CAGNNNCTG	1 cut(s)	1081
CciI	TCATGA	2 cut(s)	19, 1015
CfoI	GCGC	2 cut(s)	783, 900
Cfr10I	RCCGGY	1 cut(s)	777
Cfr13I	GGNCC	2 cut(s)	237, 487
Csp6I	GTAC	4 cut(s)	202, 318, 769, 1023
CspCI	CAANNNNNGTGG	2 cut(s)	689, 724
CviQI	GTAC	4 cut(s)	202, 318, 769, 1023
DdeI	CTNAG	6 cut(s)	462, 538, 570, 576, 765, 921
DpnI	GATC	5 cut(s)	181, 801, 872, 1074, 1197
DpnII	GATC	5 cut(s)	179, 799, 870, 1072, 1195
DraIII	CACNNNGTG	1 cut(s)	437
Eam1104I	CTCTTC	1 cut(s)	444
EarI	CTCTTC	1 cut(s)	444
Eco130I	CCWWGG	1 cut(s)	44
Eco24I	GRGCYC	1 cut(s)	1056
Eco57I	CTGAAG	1 cut(s)	422
Eco88I	CYCGRG	1 cut(s)	852
EcoRI	GAATTC	1 cut(s)	244
EcoRII	CCWGG	2 cut(s)	313, 1047
EcoT14I	CCWWGG	1 cut(s)	44
EcoT38I	GRGCYC	1 cut(s)	1056
ErhI	CCWWGG	1 cut(s)	44
FaqI	GGGAC	2 cut(s)	869, 985
FbaI	TGATCA	1 cut(s)	1072
Fnu4HI	GCNGC	5 cut(s)	565, 595, 598, 696, 1175
FriOI	GRGCYC	1 cut(s)	1056
Fsp4HI	GCNGC	5 cut(s)	565, 595, 598, 696, 1175
FspBI	CTAG	1 cut(s)	45
FspI	TGCGCA	1 cut(s)	899
GlaI	GCGC	2 cut(s)	782, 899
GluI	GCNGC	5 cut(s)	565, 595, 598, 696, 1175
HaeII	RGCGCY	1 cut(s)	784
HaeIII	GGCC	3 cut(s)	130, 239, 488
HapII	CCGG	4 cut(s)	138, 240, 778, 906
HhaI	GCGC	2 cut(s)	783, 900
Hin6I	GCGC	2 cut(s)	781, 898
HinP1I	GCGC	2 cut(s)	781, 898
HindIII	AAGCTT	1 cut(s)	278
HinfI	GANTC	8 cut(s)	92, 358, 452, 458, 761, 839, 848, 1106
HpaII	CCGG	4 cut(s)	138, 240, 778, 906
HphI	GGTGA	3 cut(s)	110, 152, 1267
Hpy188I	TCNGA	7 cut(s)	457, 469, 653, 719, 766, 1111, 1195
Hpy99I	CGWCG	1 cut(s)	1209
HpyAV	CCTTC	2 cut(s)	95, 1177
HpyCH4III	ACNGT	2 cut(s)	378, 739
HpyCH4IV	ACGT	3 cut(s)	937, 1068, 1204
HpyF10VI	GCNNNNNNNGC	3 cut(s)	36, 389, 897
HpyF3I	CTNAG	6 cut(s)	462, 538, 570, 576, 765, 921
HpySE526I	ACGT	3 cut(s)	937, 1068, 1204
HspAI	GCGC	2 cut(s)	781, 898
KpnI	GGTACC	1 cut(s)	205
KroI	GCCGGC	1 cut(s)	777
KroNI	GCCGGC	1 cut(s)	779
Ksp22I	TGATCA	1 cut(s)	1072
Kzo9I	GATC	5 cut(s)	179, 799, 870, 1072, 1195
LmnI	GCTCC	3 cut(s)	121, 1051, 1297
Lsp1109I	GCAGC	5 cut(s)	551, 581, 584, 682, 1186
LweI	GCATC	7 cut(s)	314, 350, 513, 710, 778, 900, 1216
MaeI	CTAG	1 cut(s)	45
MaeII	ACGT	3 cut(s)	937, 1068, 1204
MaeIII	GTNAC	2 cut(s)	372, 1036
MalI	GATC	5 cut(s)	181, 801, 872, 1074, 1197
MboI	GATC	5 cut(s)	179, 799, 870, 1072, 1195
MboII	GAAGA	9 cut(s)	203, 367, 461, 625, 880, 995, 1013, 1076, 1159
MflI	RGATCY	3 cut(s)	179, 799, 870
MhlI	GDGCHC	2 cut(s)	715, 1056
MluCI	AATT	6 cut(s)	124, 244, 332, 444, 622, 1354
MlyI	GAGTC	3 cut(s)	101, 461, 1115
MmeI	TCCRAC	1 cut(s)	495
MroNI	GCCGGC	1 cut(s)	777
MroXI	GAANNNNTTC	2 cut(s)	1134, 1324
MseI	TTAA	3 cut(s)	123, 875, 1266
MslI	CAYNNNNRTG	2 cut(s)	349, 834
MspA1I	CMGCKG	2 cut(s)	365, 416
MspI	CCGG	4 cut(s)	138, 240, 778, 906
MspR9I	CCNGG	5 cut(s)	138, 241, 315, 907, 1049
Mva1269I	GAATGC	1 cut(s)	600
MvaI	CCWGG	2 cut(s)	315, 1049
MwoI	GCNNNNNNNGC	3 cut(s)	36, 389, 897
NaeI	GCCGGC	1 cut(s)	779
NciI	CCSGG	3 cut(s)	138, 241, 907
NdeII	GATC	5 cut(s)	179, 799, 870, 1072, 1195
NgoMIV	GCCGGC	1 cut(s)	777
NlaIV	GGNNCC	4 cut(s)	203, 238, 807, 1053
NmuCI	GTSAC	2 cut(s)	372, 1036
NsbI	TGCGCA	1 cut(s)	899
NspI	RCATGY	5 cut(s)	410, 695, 815, 837, 1088
NspV	TTCGAA	1 cut(s)	611
OliI	CACNNNNGTG	1 cut(s)	834
PaeI	GCATGC	2 cut(s)	695, 1088
PagI	TCATGA	2 cut(s)	19, 1015
PciI	ACATGT	2 cut(s)	811, 833
PctI	GAATGC	1 cut(s)	600
PdiI	GCCGGC	1 cut(s)	779
PdmI	GAANNNNTTC	2 cut(s)	1134, 1324
PfeI	GAWTC	5 cut(s)	358, 458, 761, 839, 848
PfoI	TCCNGGA	1 cut(s)	905
PkrI	GCNGC	5 cut(s)	566, 596, 599, 697, 1176
PleI	GAGTC	3 cut(s)	100, 460, 1114
PpsI	GAGTC	3 cut(s)	100, 460, 1114
PscI	ACATGT	2 cut(s)	811, 833
Psp6I	CCWGG	2 cut(s)	313, 1047
PspGI	CCWGG	2 cut(s)	313, 1047
PspN4I	GGNNCC	4 cut(s)	203, 238, 807, 1053
PspPI	GGNCC	2 cut(s)	237, 487
PstNI	CAGNNNCTG	1 cut(s)	1081
PsuI	RGATCY	3 cut(s)	179, 799, 870
PvuII	CAGCTG	1 cut(s)	416
RsaI	GTAC	4 cut(s)	203, 319, 770, 1024
RsaNI	GTAC	4 cut(s)	202, 318, 769, 1023
RseI	CAYNNNNRTG	2 cut(s)	349, 834
SaqAI	TTAA	3 cut(s)	123, 875, 1266
SatI	GCNGC	5 cut(s)	565, 595, 598, 696, 1175
Sau3AI	GATC	5 cut(s)	179, 799, 870, 1072, 1195
Sau96I	GGNCC	2 cut(s)	237, 487
ScaI	AGTACT	2 cut(s)	770, 1024
SchI	GAGTC	3 cut(s)	101, 461, 1115
ScrFI	CCNGG	5 cut(s)	138, 241, 315, 907, 1049
SduI	GDGCHC	2 cut(s)	715, 1056
SfaNI	GCATC	7 cut(s)	314, 350, 513, 710, 778, 900, 1216
SfcI	CTRYAG	1 cut(s)	738
SfuI	TTCGAA	1 cut(s)	611
SmiMI	CAYNNNNRTG	2 cut(s)	349, 834
SmlI	CTYRAG	2 cut(s)	1178, 1286
SmoI	CTYRAG	2 cut(s)	1178, 1286
SphI	GCATGC	2 cut(s)	695, 1088
Sse9I	AATT	6 cut(s)	124, 244, 332, 444, 622, 1354
SsiI	CCGC	2 cut(s)	151, 365
SspI	AATATT	1 cut(s)	880
SspMI	CTAG	1 cut(s)	45
StyD4I	CCNGG	5 cut(s)	136, 239, 313, 905, 1047
StyI	CCWWGG	1 cut(s)	44
TaaI	ACNGT	2 cut(s)	378, 739
TaiI	ACGT	3 cut(s)	940, 1071, 1207
TaqI	TCGA	3 cut(s)	586, 611, 1236
TasI	AATT	6 cut(s)	124, 244, 332, 444, 622, 1354
TatI	WGTACW	2 cut(s)	768, 1022
TfiI	GAWTC	5 cut(s)	358, 458, 761, 839, 848
Tru1I	TTAA	3 cut(s)	123, 875, 1266
Tru9I	TTAA	3 cut(s)	123, 875, 1266
TscAI	CASTG	4 cut(s)	381, 574, 669, 742
TseFI	GTSAC	2 cut(s)	372, 1036
TseI	GCWGC	5 cut(s)	564, 594, 597, 695, 1174
Tsp45I	GTSAC	2 cut(s)	372, 1036
TspDTI	ATGAA	5 cut(s)	8, 69, 535, 1032, 1077
TspRI	CASTG	4 cut(s)	381, 574, 669, 742
XapI	RAATTY	3 cut(s)	244, 332, 622
XceI	RCATGY	5 cut(s)	410, 695, 815, 837, 1088
XmaJI	CCTAGG	1 cut(s)	44
XmnI	GAANNNNTTC	2 cut(s)	1134, 1324
XspI	CTAG	1 cut(s)	45
ZrmI	AGTACT	2 cut(s)	770, 1024

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.