FvH4_4g16340

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012372
Physical Location & Seq
Forward (+)
20324843 .. 20327262
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16340.t1

Sequence Viewer

Length: 426 bp
ATGGCTGCAGCAGTAGCACAAGGAGTCAGTCTGAACCACATTTCCCGAGAATCCTCGGACATCAGGCGCCTCGCAGAGTTCTACAAAGAGATATTTGGGTTCGAGGAGATAGAGAGCCCAAACTTTGGGGAATTCAAGGTGATATGGCTGAACTTACCCTCCACTTTTTCTATGCACCTAATCGAGAGGAACCCAACTTACCAGCTTCCTGAAGGACCCTGGAGCGCCACGTCACCTGTTGCCGACCCCAGCCACCTTCCCAGAGGCCACCATATCTGCTTCTCTGTCTCCAATTTCGACTCTTTTGTGCAAACACTCAAGGAAAAGGGAATTCAAACTTTTCAGAGGTCACTACCTAATGGCAAGGTTAGGCAGGTCTTTTTCTTTGATCCAGATGGCAACGGTTTGGAGGTTGCAGGACAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_4

Protein Analysis

142

Amino Acids

15.87

Weight (kDa)

5.68

Isoelectric Point (pI)

59.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 11 - 138 1.3e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 13 - 122 5e-08 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012372)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32090 AT2G32090
fragaria_vesca FvH4_4g16340 FvH4_4g16340
malus_domestica MD13G1194100.v1.1
prunus_persica Prupe.1G014900_v2.0.a1 Prupe.1G014900_v2.0.a1
pyrus_communis pycom13g16640
rosa_chinensis RchiOBHm_Chr4g0416051
rosa_laevigata RLG00000008022
rosa_multiflora Rmu_sc0007573.1_g000005 Rmu_sc0007573.1_g000007
rosa_roxburghii Rroxscaffold_5G00359880
rosa_rugosa Rorug04G0137700
rosa_samantha Rh4AG200000 Rh4BG197600 Rh4CG211300 Rh4DG197800
rosa_wichuraiana Rw4G016980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 364
AccB1I GGYRCC 1 cut(s) 66
AccB7I CCANNNNNTGG 1 cut(s) 125
AclWI GGATC 1 cut(s) 383
AcsI RAATTY 2 cut(s) 131, 330
AcuI CTGAAG 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 125
AgsI TTSAA 2 cut(s) 136, 335
AjiI CACGTC 1 cut(s) 231
AjnI CCWGG 1 cut(s) 218
AloI GAACNNNNNNTCC 4 cut(s) 26, 58, 143, 175
AluBI AGCT 1 cut(s) 205
AluI AGCT 1 cut(s) 205
Alw26I GTCTC 1 cut(s) 292
AlwI GGATC 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 45
AoxI GGCC 1 cut(s) 265
ApeKI GCWGC 2 cut(s) 5, 8
ApoI RAATTY 2 cut(s) 131, 330
AspLEI GCGC 2 cut(s) 69, 227
AspS9I GGNCC 1 cut(s) 215
AsuHPI GGTGA 2 cut(s) 151, 225
AvaI CYCGRG 1 cut(s) 45
AvaII GGWCC 1 cut(s) 215
BanI GGYRCC 1 cut(s) 66
BanII GRGCYC 1 cut(s) 119
BbvI GCAGC 1 cut(s) 20
BccI CCATC 1 cut(s) 389
BciT130I CCWGG 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 292
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 2 cut(s) 70, 228
BfuAI ACCTGC 1 cut(s) 364
BisI GCNGC 2 cut(s) 6, 9
BlsI GCNGC 2 cut(s) 7, 10
Bme1390I CCNGG 1 cut(s) 220
Bme18I GGWCC 1 cut(s) 215
BmeT110I CYCGRG 1 cut(s) 45
BmgBI CACGTC 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 215
BmiI GGNNCC 3 cut(s) 68, 191, 217
BmrFI CCNGG 1 cut(s) 220
BpmI CTGGAG 1 cut(s) 241
BpuEI CTTGAG 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 54, 218
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 125
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 2 cut(s) 54, 218
BseLI CCNNNNNNNGG 1 cut(s) 125
BseRI GAGGAG 1 cut(s) 119
BseXI GCAGC 1 cut(s) 20
BseYI CCCAGC 1 cut(s) 248
BshFI GGCC 1 cut(s) 267
BshNI GGYRCC 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 45
BslI CCNNNNNNNGG 1 cut(s) 125
BsmAI GTCTC 1 cut(s) 292
BsnI GGCC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 119
Bsp143I GATC 1 cut(s) 388
BspANI GGCC 1 cut(s) 267
BspLI GGNNCC 3 cut(s) 68, 191, 217
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 364
BspPI GGATC 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 66
BssECI CCNNGG 2 cut(s) 54, 218
BssMI GATC 1 cut(s) 388
BssNI GRCGYC 1 cut(s) 67
Bst2UI CCWGG 1 cut(s) 220
Bst4CI ACNGT 2 cut(s) 404, 423
BstACI GRCGYC 1 cut(s) 67
BstH2I RGCGCY 2 cut(s) 70, 228
BstHHI GCGC 2 cut(s) 69, 227
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNI CCWGG 1 cut(s) 220
BstSCI CCNGG 1 cut(s) 218
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 1 cut(s) 20
BsuRI GGCC 1 cut(s) 267
BtrI CACGTC 1 cut(s) 231
BveI ACCTGC 1 cut(s) 364
CfoI GCGC 2 cut(s) 69, 227
Cfr13I GGNCC 1 cut(s) 215
CviJI RGCY 6 cut(s) 5, 117, 148, 205, 252, 267
CviKI_1 RGCY 6 cut(s) 5, 117, 148, 205, 252, 267
DinI GGCGCC 1 cut(s) 68
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 231
Eco88I CYCGRG 1 cut(s) 45
EcoO109I RGGNCCY 1 cut(s) 215
EcoRI GAATTC 2 cut(s) 131, 330
EcoRII CCWGG 1 cut(s) 218
EcoT38I GRGCYC 1 cut(s) 119
EgeI GGCGCC 1 cut(s) 68
EheI GGCGCC 1 cut(s) 68
FaiI YATR 3 cut(s) 145, 173, 273
Fnu4HI GCNGC 2 cut(s) 6, 9
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 2 cut(s) 6, 9
GlaI GCGC 2 cut(s) 68, 226
GluI GCNGC 2 cut(s) 6, 9
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 1 cut(s) 241
HaeII RGCGCY 2 cut(s) 70, 228
HaeIII GGCC 1 cut(s) 267
HhaI GCGC 2 cut(s) 69, 227
Hin1I GRCGYC 1 cut(s) 67
Hin6I GCGC 2 cut(s) 67, 225
HinP1I GCGC 2 cut(s) 67, 225
HinfI GANTC 3 cut(s) 24, 50, 299
HphI GGTGA 2 cut(s) 151, 225
Hpy188I TCNGA 3 cut(s) 33, 58, 345
Hpy188III TCNNGA 4 cut(s) 45, 184, 209, 392
HpyAV CCTTC 2 cut(s) 206, 266
HpyCH4III ACNGT 2 cut(s) 404, 423
HpyCH4IV ACGT 1 cut(s) 230
HpyCH4V TGCA 4 cut(s) 8, 175, 310, 416
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpySE526I ACGT 1 cut(s) 230
Hsp92I GRCGYC 1 cut(s) 67
HspAI GCGC 2 cut(s) 67, 225
KasI GGCGCC 1 cut(s) 66
Kzo9I GATC 1 cut(s) 388
LmnI GCTCC 1 cut(s) 222
Lsp1109I GCAGC 1 cut(s) 20
MaeII ACGT 1 cut(s) 230
MaeIII GTNAC 2 cut(s) 231, 348
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 119
MluCI AATT 3 cut(s) 131, 292, 330
Mly113I GGCGCC 1 cut(s) 67
MlyI GAGTC 2 cut(s) 33, 293
MnlI CCTC 8 cut(s) 64, 80, 97, 169, 180, 257, 339, 403
MspR9I CCNGG 1 cut(s) 220
MvaI CCWGG 1 cut(s) 220
MwoI GCNNNNNNNGC 1 cut(s) 14
NarI GGCGCC 1 cut(s) 67
NdeII GATC 1 cut(s) 388
NlaIV GGNNCC 3 cut(s) 68, 191, 217
NmuCI GTSAC 2 cut(s) 231, 348
PfeI GAWTC 1 cut(s) 50
PflMI CCANNNNNTGG 1 cut(s) 125
PkrI GCNGC 2 cut(s) 7, 10
PleI GAGTC 2 cut(s) 32, 293
PluTI GGCGCC 1 cut(s) 70
PpsI GAGTC 2 cut(s) 32, 293
PpuMI RGGWCCY 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 218
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 3 cut(s) 68, 191, 217
PspPI GGNCC 1 cut(s) 215
PspPPI RGGWCCY 1 cut(s) 215
PsrI GAACNNNNNNTAC 2 cut(s) 182, 214
PstI CTGCAG 1 cut(s) 10
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 1 cut(s) 215
SchI GAGTC 2 cut(s) 33, 293
ScrFI CCNGG 1 cut(s) 220
SduI GDGCHC 1 cut(s) 119
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 68
SinI GGWCC 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 3 cut(s) 131, 292, 330
SspDI GGCGCC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 218
TaaI ACNGT 2 cut(s) 404, 423
TaiI ACGT 1 cut(s) 233
TaqI TCGA 3 cut(s) 102, 183, 297
TasI AATT 3 cut(s) 131, 292, 330
TfiI GAWTC 1 cut(s) 50
TseFI GTSAC 2 cut(s) 231, 348
TseI GCWGC 2 cut(s) 5, 8
Tsp45I GTSAC 2 cut(s) 231, 348
Van91I CCANNNNNTGG 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 2 cut(s) 131, 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.