RLG00000008022

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012372
Physical Location & Seq
Reverse (-)
24455874 .. 24459847
3974 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008022

Sequence Viewer

Length: 723 bp
ATGGCTGCAGCACCAGTAGCACAAGGAGTGAGTCTGAACCACATTTCCAGAGAATCCTCGGACATTAGGCGCCTCGCCGAGTTCTACAAAGAGACATTTGGGTTCGAGGAGATAGAGAGCCCAAACTTTGGGGAATTCAAGGTGATATGGCTGAATTTACCTTCAGCTTTTTCTATGCACCTTATCGAGAGGAACCCAACTTACCAGCTTCCTGAAGGACCCTGGAGCGCCACGTCACCTGTTGCCGACCCAAGCCACCTTCCCAGAGGCCACCATATCTGCTTCTCTGTCTCCAATTTCGAATCTTTTGTGCAAACACTCAAGGAAAAGGGAATTCAGACTTTTCAGAGGTCACTACCTAATGGCAAGGTTAAGCAAGTATTTTTCTTTGATCCCGATGGCAACGGTTTGGAGGTTGCAGAGTCAGCGTGGGCCTTTGGCTCTGCTAATGTGTGGGGTTTGCTGCCGAACGAGTTTTGGGAGGCTCTGTGGTGGTTGAGGCGGTGCGTTGCTGAACTAGATGGCGTCATGCCTGACAGGAAGACGGGACCGGTGGCCTTCTGCATTGGATATGGACGACGATGGTTGTGGGTGAAGATAGGCAAGGAAGCATGCTCAGGCATGGGTCGCTATGGTGGTGGAGTATACAGTTTTGCTTGGTGTCCCATCGGTGTTCTAGATCTCTTGGAGGGGAAGACAGTGCCGTTTTATGTTCGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_4

Protein Analysis

241

Amino Acids

27.02

Weight (kDa)

6.23

Isoelectric Point (pI)

56.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 12 - 139 7.1e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 14 - 123 4.7e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012372)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32090 AT2G32090
fragaria_vesca FvH4_4g16340 FvH4_4g16340
malus_domestica MD13G1194100.v1.1
prunus_persica Prupe.1G014900_v2.0.a1 Prupe.1G014900_v2.0.a1
pyrus_communis pycom13g16640
rosa_chinensis RchiOBHm_Chr4g0416051
rosa_laevigata RLG00000008022
rosa_multiflora Rmu_sc0007573.1_g000005 Rmu_sc0007573.1_g000007
rosa_roxburghii Rroxscaffold_5G00359880
rosa_rugosa Rorug04G0137700
rosa_samantha Rh4AG200000 Rh4BG197600 Rh4CG211300 Rh4DG197800
rosa_wichuraiana Rw4G016980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 69
AccB7I CCANNNNNTGG 1 cut(s) 128
AccI GTMKAC 1 cut(s) 645
AciI CCGC 1 cut(s) 502
AclWI GGATC 1 cut(s) 386
AcsI RAATTY 3 cut(s) 134, 154, 333
AcuI CTGAAG 2 cut(s) 147, 234
AcyI GRCGYC 2 cut(s) 70, 525
AfiI CCNNNNNNNGG 1 cut(s) 128
AgeI ACCGGT 1 cut(s) 550
AgsI TTSAA 1 cut(s) 139
AjiI CACGTC 1 cut(s) 234
AjnI CCWGG 1 cut(s) 221
AloI GAACNNNNNNTCC 2 cut(s) 29, 61
AluBI AGCT 2 cut(s) 167, 208
AluI AGCT 2 cut(s) 167, 208
Alw26I GTCTC 2 cut(s) 86, 295
AlwI GGATC 1 cut(s) 386
AoxI GGCC 3 cut(s) 268, 432, 555
ApeKI GCWGC 3 cut(s) 5, 8, 463
ApoI RAATTY 3 cut(s) 134, 154, 333
AsiGI ACCGGT 1 cut(s) 550
AspLEI GCGC 2 cut(s) 72, 230
AspS9I GGNCC 3 cut(s) 218, 432, 548
AsuHPI GGTGA 3 cut(s) 154, 228, 604
AsuII TTCGAA 1 cut(s) 300
AvaII GGWCC 2 cut(s) 218, 548
BanI GGYRCC 1 cut(s) 69
BanII GRGCYC 1 cut(s) 122
BbsI GAAGAC 2 cut(s) 548, 701
BbvI GCAGC 2 cut(s) 20, 450
BccI CCATC 4 cut(s) 392, 515, 576, 674
BceAI ACGGC 1 cut(s) 688
BciT130I CCWGG 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 86, 295
BfaI CTAG 2 cut(s) 518, 677
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 2 cut(s) 73, 231
BglII AGATCT 1 cut(s) 679
BisI GCNGC 3 cut(s) 6, 9, 464
BlsI GCNGC 3 cut(s) 7, 10, 465
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 2 cut(s) 218, 548
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 3 cut(s) 218, 432, 548
BmiI GGNNCC 4 cut(s) 71, 194, 220, 549
BmrFI CCNGG 1 cut(s) 223
BpiI GAAGAC 2 cut(s) 548, 701
BpmI CTGGAG 1 cut(s) 244
Bpu10I CCTNAGC 1 cut(s) 616
Bpu14I TTCGAA 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 305
BsaHI GRCGYC 2 cut(s) 70, 525
BsaJI CCNNGG 2 cut(s) 57, 221
BsaWI WCCGGW 1 cut(s) 550
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse118I RCCGGY 1 cut(s) 550
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 2 cut(s) 57, 221
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 1 cut(s) 122
BseXI GCAGC 2 cut(s) 20, 450
BshFI GGCC 3 cut(s) 270, 434, 557
BshNI GGYRCC 1 cut(s) 69
BshTI ACCGGT 1 cut(s) 550
BsiSI CCGG 1 cut(s) 551
BslFI GGGAC 2 cut(s) 561, 648
BslI CCNNNNNNNGG 1 cut(s) 128
BsmAI GTCTC 2 cut(s) 86, 295
BsmFI GGGAC 2 cut(s) 561, 648
BsnI GGCC 3 cut(s) 270, 434, 557
Bsp119I TTCGAA 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 122
Bsp143I GATC 2 cut(s) 391, 679
BspACI CCGC 1 cut(s) 502
BspANI GGCC 3 cut(s) 270, 434, 557
BspCNI CTCAG 1 cut(s) 629
BspLI GGNNCC 4 cut(s) 71, 194, 220, 549
BspMAI CTGCAG 1 cut(s) 10
BspPI GGATC 1 cut(s) 386
BspT104I TTCGAA 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 550
BsrI ACTGG 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 2 cut(s) 57, 221
BssMI GATC 2 cut(s) 391, 679
BssNAI GTATAC 1 cut(s) 646
BssNI GRCGYC 2 cut(s) 70, 525
Bst1107I GTATAC 1 cut(s) 646
Bst2UI CCWGG 1 cut(s) 223
Bst4CI ACNGT 3 cut(s) 407, 650, 700
BstACI GRCGYC 2 cut(s) 70, 525
BstBI TTCGAA 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 613
BstDEI CTNAG 1 cut(s) 616
BstH2I RGCGCY 2 cut(s) 73, 231
BstHHI GCGC 2 cut(s) 72, 230
BstKTI GATC 2 cut(s) 394, 682
BstMAI GTCTC 2 cut(s) 86, 295
BstMBI GATC 2 cut(s) 391, 679
BstMWI GCNNNNNNNGC 3 cut(s) 17, 425, 627
BstNI CCWGG 1 cut(s) 223
BstNSI RCATGY 1 cut(s) 615
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 2 cut(s) 20, 450
BstV2I GAAGAC 2 cut(s) 548, 701
BstX2I RGATCY 1 cut(s) 679
BstYI RGATCY 1 cut(s) 679
BstZ17I GTATAC 1 cut(s) 646
BsuRI GGCC 3 cut(s) 270, 434, 557
BtrI CACGTC 1 cut(s) 234
BtsIMutI CAGTG 1 cut(s) 705
Cac8I GCNNGC 1 cut(s) 613
CfoI GCGC 2 cut(s) 72, 230
Cfr10I RCCGGY 1 cut(s) 550
Cfr13I GGNCC 3 cut(s) 218, 432, 548
CseI GACGC 1 cut(s) 514
CspAI ACCGGT 1 cut(s) 550
CviAII CATG 3 cut(s) 529, 612, 622
DdeI CTNAG 1 cut(s) 616
DinI GGCGCC 1 cut(s) 71
DpnI GATC 2 cut(s) 393, 681
DpnII GATC 2 cut(s) 391, 679
Eco24I GRGCYC 1 cut(s) 122
Eco47I GGWCC 2 cut(s) 218, 548
Eco57I CTGAAG 2 cut(s) 147, 234
EcoO109I RGGNCCY 1 cut(s) 218
EcoRI GAATTC 2 cut(s) 134, 333
EcoRII CCWGG 1 cut(s) 221
EcoT38I GRGCYC 1 cut(s) 122
EgeI GGCGCC 1 cut(s) 71
EheI GGCGCC 1 cut(s) 71
FaeI CATG 3 cut(s) 532, 615, 625
FaqI GGGAC 2 cut(s) 561, 648
FatI CATG 3 cut(s) 528, 611, 621
FblI GTMKAC 1 cut(s) 645
Fnu4HI GCNGC 3 cut(s) 6, 9, 464
FriOI GRGCYC 1 cut(s) 122
Fsp4HI GCNGC 3 cut(s) 6, 9, 464
FspBI CTAG 2 cut(s) 518, 677
GlaI GCGC 2 cut(s) 71, 229
GluI GCNGC 3 cut(s) 6, 9, 464
GsuI CTGGAG 1 cut(s) 244
HaeII RGCGCY 2 cut(s) 73, 231
HaeIII GGCC 3 cut(s) 270, 434, 557
HapII CCGG 1 cut(s) 551
HgaI GACGC 1 cut(s) 514
HhaI GCGC 2 cut(s) 72, 230
Hin1I GRCGYC 2 cut(s) 70, 525
Hin1II CATG 3 cut(s) 532, 615, 625
Hin6I GCGC 2 cut(s) 70, 228
HinP1I GCGC 2 cut(s) 70, 228
HinfI GANTC 4 cut(s) 31, 53, 302, 422
HpaII CCGG 1 cut(s) 551
HphI GGTGA 3 cut(s) 154, 228, 604
Hpy166II GTNNAC 1 cut(s) 646
Hpy188I TCNGA 4 cut(s) 36, 61, 339, 348
Hpy188III TCNNGA 5 cut(s) 48, 187, 212, 395, 677
Hpy8I GTNNAC 1 cut(s) 646
Hpy99I CGWCG 1 cut(s) 582
HpyAV CCTTC 4 cut(s) 171, 209, 269, 568
HpyCH4III ACNGT 3 cut(s) 407, 650, 700
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 5 cut(s) 8, 178, 313, 419, 564
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 425, 627
HpyF3I CTNAG 1 cut(s) 616
HpySE526I ACGT 1 cut(s) 233
Hsp92I GRCGYC 2 cut(s) 70, 525
Hsp92II CATG 3 cut(s) 532, 615, 625
HspAI GCGC 2 cut(s) 70, 228
KasI GGCGCC 1 cut(s) 69
Kzo9I GATC 2 cut(s) 391, 679
LmnI GCTCC 1 cut(s) 225
Lsp1109I GCAGC 2 cut(s) 20, 450
MaeI CTAG 2 cut(s) 518, 677
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 2 cut(s) 234, 351
MalI GATC 2 cut(s) 393, 681
MboI GATC 2 cut(s) 391, 679
MboII GAAGA 3 cut(s) 553, 607, 706
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 4 cut(s) 134, 154, 295, 333
Mly113I GGCGCC 1 cut(s) 70
MlyI GAGTC 2 cut(s) 40, 431
MseI TTAA 1 cut(s) 372
MspI CCGG 1 cut(s) 551
MspR9I CCNGG 1 cut(s) 223
MvaI CCWGG 1 cut(s) 223
MwoI GCNNNNNNNGC 3 cut(s) 17, 425, 627
NarI GGCGCC 1 cut(s) 70
NdeII GATC 2 cut(s) 391, 679
NlaIII CATG 3 cut(s) 532, 615, 625
NlaIV GGNNCC 4 cut(s) 71, 194, 220, 549
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 2 cut(s) 234, 351
NspI RCATGY 1 cut(s) 615
NspV TTCGAA 1 cut(s) 300
PaeI GCATGC 1 cut(s) 615
PfeI GAWTC 2 cut(s) 53, 302
PflMI CCANNNNNTGG 1 cut(s) 128
PinAI ACCGGT 1 cut(s) 550
PkrI GCNGC 3 cut(s) 7, 10, 465
PleI GAGTC 2 cut(s) 39, 430
PluTI GGCGCC 1 cut(s) 73
PpsI GAGTC 2 cut(s) 39, 430
PpuMI RGGWCCY 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspN4I GGNNCC 4 cut(s) 71, 194, 220, 549
PspPI GGNCC 3 cut(s) 218, 432, 548
PspPPI RGGWCCY 1 cut(s) 218
PsrI GAACNNNNNNTAC 2 cut(s) 185, 217
PstI CTGCAG 1 cut(s) 10
PsuI RGATCY 1 cut(s) 679
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 3 cut(s) 6, 9, 464
Sau3AI GATC 2 cut(s) 391, 679
Sau96I GGNCC 3 cut(s) 218, 432, 548
SchI GAGTC 2 cut(s) 40, 431
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 1 cut(s) 122
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 71
SfuI TTCGAA 1 cut(s) 300
SinI GGWCC 2 cut(s) 218, 548
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SphI GCATGC 1 cut(s) 615
Sse9I AATT 4 cut(s) 134, 154, 295, 333
SsiI CCGC 1 cut(s) 502
SspDI GGCGCC 1 cut(s) 69
SspMI CTAG 2 cut(s) 518, 677
StyD4I CCNGG 1 cut(s) 221
TaaI ACNGT 3 cut(s) 407, 650, 700
TaiI ACGT 1 cut(s) 236
TaqI TCGA 3 cut(s) 105, 186, 300
TasI AATT 4 cut(s) 134, 154, 295, 333
TfiI GAWTC 2 cut(s) 53, 302
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 705
TseFI GTSAC 2 cut(s) 234, 351
TseI GCWGC 3 cut(s) 5, 8, 463
Tsp45I GTSAC 2 cut(s) 234, 351
TspRI CASTG 1 cut(s) 705
Van91I CCANNNNNTGG 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 218, 548
XapI RAATTY 3 cut(s) 134, 154, 333
XbaI TCTAGA 1 cut(s) 676
XceI RCATGY 1 cut(s) 615
XmiI GTMKAC 1 cut(s) 645
XspI CTAG 2 cut(s) 518, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.