Rroxscaffold_5G00359880

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012372
Physical Location & Seq
Forward (+)
40188371 .. 40191145
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00359880.1

Sequence Viewer

Length: 534 bp
ATGGCTGCAGCGCCAGTAGCACAAGGAGTGAGTCTGAACCACATTTCCAGAGAATCCTCGGACATTAGGCGCCTCGCCGAGTTCTACAAAGAGACATTTGGGTTCGAGGAGATTGAGAGCCCAAACTTCGGGGAATTCAAGGTGATATGGCTGAATTTACCTTCAGCTTTTTCTATGCACCTTATCGAGAGGAACCCAACTTACCAGCTTCCTGAAGGACCCTGGAGCGCCACGTCACCTGTTGCCGACCCAAGCCACCTTCCCAGGGGTCACCACATCTGCTTCTCTGTTTCCAATTTCGAATCTTTTGTGCAGACACTCAAGTATCTTATAAATGAGGTCTATGCTATGTTTGAAACAAGTATGCAACCTCAATTGTTTGCTCATCATCTTCCTCGTTTTCTGCTTCGCTCTGCTTCTTTTGGCGCTTTGTTCAGCATTTCCTATTCAAAGATGAAGCAACGGTTTGGAGGTTGCAGGTCAATAAGCTCCATGGCGGCACAGAGGGTTGCTCGAAGTGGTAAAGGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_4

Protein Analysis

177

Amino Acids

19.94

Weight (kDa)

8.62

Isoelectric Point (pI)

63.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012372)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32090 AT2G32090
fragaria_vesca FvH4_4g16340 FvH4_4g16340
malus_domestica MD13G1194100.v1.1
prunus_persica Prupe.1G014900_v2.0.a1 Prupe.1G014900_v2.0.a1
pyrus_communis pycom13g16640
rosa_chinensis RchiOBHm_Chr4g0416051
rosa_laevigata RLG00000008022
rosa_multiflora Rmu_sc0007573.1_g000005 Rmu_sc0007573.1_g000007
rosa_roxburghii Rroxscaffold_5G00359880
rosa_rugosa Rorug04G0137700
rosa_samantha Rh4AG200000 Rh4BG197600 Rh4CG211300 Rh4DG197800
rosa_wichuraiana Rw4G016980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 468
AccB1I GGYRCC 1 cut(s) 69
AciI CCGC 1 cut(s) 497
AcsI RAATTY 2 cut(s) 134, 154
AcuI CTGAAG 2 cut(s) 147, 234
AcyI GRCGYC 1 cut(s) 70
AfiI CCNNNNNNNGG 2 cut(s) 128, 265
AgsI TTSAA 3 cut(s) 139, 356, 450
AjiI CACGTC 1 cut(s) 234
AjnI CCWGG 2 cut(s) 221, 263
AloI GAACNNNNNNTCC 2 cut(s) 29, 61
AluBI AGCT 3 cut(s) 167, 208, 489
AluI AGCT 3 cut(s) 167, 208, 489
Alw26I GTCTC 1 cut(s) 86
ApeKI GCWGC 2 cut(s) 5, 8
ApoI RAATTY 2 cut(s) 134, 154
AspLEI GCGC 4 cut(s) 13, 72, 230, 428
AspS9I GGNCC 1 cut(s) 218
AsuHPI GGTGA 3 cut(s) 154, 228, 263
AsuII TTCGAA 1 cut(s) 300
AvaII GGWCC 1 cut(s) 218
BaeI ACNNNNGTAYC 2 cut(s) 308, 341
BanI GGYRCC 1 cut(s) 69
BanII GRGCYC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 20
BciT130I CCWGG 2 cut(s) 223, 265
BcoDI GTCTC 1 cut(s) 86
BfmI CTRYAG 1 cut(s) 6
BfoI RGCGCY 4 cut(s) 14, 73, 231, 429
BfuAI ACCTGC 1 cut(s) 468
BisI GCNGC 3 cut(s) 6, 9, 498
BlsI GCNGC 3 cut(s) 7, 10, 499
Bme1390I CCNGG 2 cut(s) 223, 265
Bme18I GGWCC 1 cut(s) 218
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 3 cut(s) 71, 194, 220
BmrFI CCNGG 2 cut(s) 223, 265
BplI GAGNNNNNCTC 2 cut(s) 496, 528
BpmI CTGGAG 1 cut(s) 244
Bpu14I TTCGAA 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 305
BsaHI GRCGYC 1 cut(s) 70
BsaJI CCNNGG 5 cut(s) 57, 221, 263, 264, 492
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 265
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 2 cut(s) 223, 265
BseDI CCNNGG 5 cut(s) 57, 221, 263, 264, 492
BseLI CCNNNNNNNGG 2 cut(s) 128, 265
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 1 cut(s) 122
BseXI GCAGC 1 cut(s) 20
BsgI GTGCAG 1 cut(s) 332
BshNI GGYRCC 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 128, 265
BsmAI GTCTC 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 122
Bsp19I CCATGG 1 cut(s) 492
BspACI CCGC 1 cut(s) 497
BspLI GGNNCC 3 cut(s) 71, 194, 220
BspMAI CTGCAG 1 cut(s) 10
BspMI ACCTGC 1 cut(s) 468
BspT104I TTCGAA 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 69
BsrI ACTGG 1 cut(s) 14
BssECI CCNNGG 5 cut(s) 57, 221, 263, 264, 492
BssNI GRCGYC 1 cut(s) 70
BssT1I CCWWGG 1 cut(s) 492
Bst2UI CCWGG 2 cut(s) 223, 265
Bst4CI ACNGT 1 cut(s) 465
BstACI GRCGYC 1 cut(s) 70
BstBI TTCGAA 1 cut(s) 300
BstDSI CCRYGG 1 cut(s) 492
BstEII GGTNACC 1 cut(s) 269
BstH2I RGCGCY 4 cut(s) 14, 73, 231, 429
BstHHI GCGC 4 cut(s) 13, 72, 230, 428
BstMAI GTCTC 1 cut(s) 86
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNI CCWGG 2 cut(s) 223, 265
BstPI GGTNACC 1 cut(s) 269
BstSCI CCNGG 2 cut(s) 221, 263
BstSFI CTRYAG 1 cut(s) 6
BstV1I GCAGC 1 cut(s) 20
BtgI CCRYGG 1 cut(s) 492
BtrI CACGTC 1 cut(s) 234
BveI ACCTGC 1 cut(s) 468
CfoI GCGC 4 cut(s) 13, 72, 230, 428
Cfr13I GGNCC 1 cut(s) 218
CviAII CATG 1 cut(s) 493
CviJI RGCY 7 cut(s) 5, 120, 151, 167, 208, 255, 489
CviKI_1 RGCY 7 cut(s) 5, 120, 151, 167, 208, 255, 489
DinI GGCGCC 1 cut(s) 71
Eco130I CCWWGG 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 218
Eco57I CTGAAG 2 cut(s) 147, 234
Eco91I GGTNACC 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 218
EcoO65I GGTNACC 1 cut(s) 269
EcoRI GAATTC 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 221, 263
EcoT14I CCWWGG 1 cut(s) 492
EcoT38I GRGCYC 1 cut(s) 122
EgeI GGCGCC 1 cut(s) 71
EheI GGCGCC 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 1 cut(s) 496
FaiI YATR 7 cut(s) 148, 176, 332, 345, 350, 365, 494
FatI CATG 1 cut(s) 492
Fnu4HI GCNGC 3 cut(s) 6, 9, 498
FriOI GRGCYC 1 cut(s) 122
Fsp4HI GCNGC 3 cut(s) 6, 9, 498
GlaI GCGC 4 cut(s) 12, 71, 229, 427
GluI GCNGC 3 cut(s) 6, 9, 498
GsuI CTGGAG 1 cut(s) 244
HaeII RGCGCY 4 cut(s) 14, 73, 231, 429
HhaI GCGC 4 cut(s) 13, 72, 230, 428
Hin1I GRCGYC 1 cut(s) 70
Hin1II CATG 1 cut(s) 496
Hin6I GCGC 4 cut(s) 11, 70, 228, 426
HinP1I GCGC 4 cut(s) 11, 70, 228, 426
HinfI GANTC 3 cut(s) 31, 53, 302
HphI GGTGA 3 cut(s) 154, 228, 263
Hpy188I TCNGA 2 cut(s) 36, 61
Hpy188III TCNNGA 3 cut(s) 48, 187, 212
HpyAV CCTTC 3 cut(s) 171, 209, 269
HpyCH4III ACNGT 1 cut(s) 465
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 5 cut(s) 8, 178, 313, 367, 477
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpySE526I ACGT 1 cut(s) 233
Hsp92I GRCGYC 1 cut(s) 70
Hsp92II CATG 1 cut(s) 496
HspAI GCGC 4 cut(s) 11, 70, 228, 426
KasI GGCGCC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 225, 494
Lsp1109I GCAGC 1 cut(s) 20
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 2 cut(s) 234, 269
MboII GAAGA 1 cut(s) 383
MfeI CAATTG 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 122
MluCI AATT 4 cut(s) 134, 154, 295, 374
Mly113I GGCGCC 1 cut(s) 70
MlyI GAGTC 1 cut(s) 40
MnlI CCTC 9 cut(s) 67, 83, 100, 183, 331, 381, 405, 464, 498
MspR9I CCNGG 2 cut(s) 223, 265
MunI CAATTG 1 cut(s) 374
MvaI CCWGG 2 cut(s) 223, 265
MwoI GCNNNNNNNGC 1 cut(s) 17
NarI GGCGCC 1 cut(s) 70
NcoI CCATGG 1 cut(s) 492
NlaIII CATG 1 cut(s) 496
NlaIV GGNNCC 3 cut(s) 71, 194, 220
NmeAIII GCCGAG 1 cut(s) 103
NmuCI GTSAC 2 cut(s) 234, 269
NspV TTCGAA 1 cut(s) 300
PasI CCCWGGG 1 cut(s) 264
PfeI GAWTC 2 cut(s) 53, 302
PkrI GCNGC 3 cut(s) 7, 10, 499
PleI GAGTC 1 cut(s) 39
PluTI GGCGCC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 39
PpuMI RGGWCCY 1 cut(s) 218
PsiI TTATAA 1 cut(s) 332
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 2 cut(s) 221, 263
PspEI GGTNACC 1 cut(s) 269
PspGI CCWGG 2 cut(s) 221, 263
PspN4I GGNNCC 3 cut(s) 71, 194, 220
PspPI GGNCC 1 cut(s) 218
PspPPI RGGWCCY 1 cut(s) 218
PsrI GAACNNNNNNTAC 2 cut(s) 185, 217
PstI CTGCAG 1 cut(s) 10
SatI GCNGC 3 cut(s) 6, 9, 498
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 1 cut(s) 40
ScrFI CCNGG 2 cut(s) 223, 265
SduI GDGCHC 1 cut(s) 122
SfcI CTRYAG 1 cut(s) 6
SfoI GGCGCC 1 cut(s) 71
SfuI TTCGAA 1 cut(s) 300
SinI GGWCC 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 4 cut(s) 134, 154, 295, 374
SsiI CCGC 1 cut(s) 497
SspDI GGCGCC 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 221, 263
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 1 cut(s) 465
TaiI ACGT 1 cut(s) 236
TaqI TCGA 4 cut(s) 105, 186, 300, 514
TasI AATT 4 cut(s) 134, 154, 295, 374
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 2 cut(s) 53, 302
TseFI GTSAC 2 cut(s) 234, 269
TseI GCWGC 2 cut(s) 5, 8
Tsp45I GTSAC 2 cut(s) 234, 269
TspDTI ATGAA 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 2 cut(s) 134, 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.