Rorug04G0137700

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005514
Physical Location & Seq
Forward (+)
22326675 .. 22330052
3378 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0137700.1

Sequence Viewer

Length: 1068 bp
ATGGCAATAGGCACAGCACTCTCAGAGCCTCCCAAAATCTACAGATTCCCACAATCCAAGTACATAGACTCCGTCCGGTGGCTGCCTCCTATCTCAGCCTTGGACCGCTTTGCCGTGATCGCGGTTTTCGACTCGGATTCGGACTCTTCCTCCATACAAATCCACTCTTTCACCCAAGACCCATCACCAAATCTAACCCCCGACTCGCAATGGGACTCACCCTCGCGAGTTTCCTCTCTCACGGCGTCTCAATTCAAGCACAAGTCCCTCGTGGCCGCCGCAGCGTTTTCGGGTTCCCTTCACGTCTTGTTCACCGATGCAGTGGACCCTTCGTCGTTTGAATCGGAGCTTTCGTTGCCCGAAAAGGCATTGCATGATGGCCCCATTGCTTGTGTGGATGTGATGGATAGTGGGCTTGAGTGTGTCACTGTTGGGGAAGATGGGAGGGTCAATCTAGTCAGTTTGGGGGAGTCTGAGCTCAGCTATAGGAGGATTTTCGATAGCAGTGGGTTGGTTTCGTTTACTTCGGCGAAATGGGCATCGCCGAGTGAGTTTGCTACTGGAGGATTTGGGTTTAGTCTGCAGTGGTGGGATCAGAGAAAGCCTGGTGGGGCGGTTTCTCAGTTCAAGGGTGACTGGAATCAAAGAAAAGGTTCCGGGATCGTACATTCCATTGATATTCATCCATCAAGAAAGCACACTTGTCTGGCAGGAGGCTCTTTAGGTACTGTATTTGCCTGGGATCTTAGGTGGCCACAACAGCCTCTTTTACTCTCTGGGGTTGGGTCAGGTGAAGCAAGCTGCTCACCATGTGAAAGTGAGGTTTGGGAGGTTCAGTATGACCCCTATGTAAAGTCTTCGAACGTAGGAAATGTTTCTTCACGTATTTTACCTACTATGATCTGTTCTGAGGATGGAATTATTGCTGTTGTTGAACAGGGTGAGGAACCCATTGAGCTCTCAGCAGAACCTTGTGCAATCAACAGTTTTGACATAGACCAGCAATATCCTTCTGATGTGATATACAGCCTGGAGTGGGAATCTATAGCCATCTTGACAAGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_4

Protein Analysis

355

Amino Acids

38.56

Weight (kDa)

4.59

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 122, 226
AciI CCGC 5 cut(s) 106, 122, 276, 279, 614
AclWI GGATC 3 cut(s) 600, 668, 750
AcoI YGGCCR 2 cut(s) 273, 752
AcyI GRCGYC 1 cut(s) 245
AdeI CACNNNGTG 1 cut(s) 812
AfaI GTAC 3 cut(s) 62, 666, 727
AfiI CCNNNNNNNGG 2 cut(s) 78, 1036
AgsI TTSAA 4 cut(s) 256, 341, 628, 935
AhdI GACNNNNNGTC 1 cut(s) 331
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 3 cut(s) 604, 737, 1029
AluBI AGCT 5 cut(s) 349, 478, 483, 801, 958
AluI AGCT 5 cut(s) 349, 478, 483, 801, 958
Alw21I GWGCWC 2 cut(s) 480, 960
Alw26I GTCTC 1 cut(s) 252
AlwI GGATC 3 cut(s) 600, 668, 750
AoxI GGCC 4 cut(s) 273, 379, 752, 1061
ApeKI GCWGC 3 cut(s) 82, 281, 801
Asp700I GAANNNNTTC 2 cut(s) 652, 874
AspS9I GGNCC 3 cut(s) 103, 325, 380
AsuC2I CCSGG 1 cut(s) 658
AsuHPI GGTGA 8 cut(s) 163, 177, 210, 304, 644, 798, 803, 953
AsuII TTCGAA 1 cut(s) 860
AvaII GGWCC 2 cut(s) 103, 325
BalI TGGCCA 1 cut(s) 754
BanII GRGCYC 2 cut(s) 480, 960
BauI CACGAG 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 849
Bbv12I GWGCWC 2 cut(s) 480, 960
BbvI GCAGC 3 cut(s) 69, 293, 788
BccI CCATC 7 cut(s) 190, 371, 397, 434, 694, 908, 1058
BceAI ACGGC 2 cut(s) 98, 258
BcgI CGANNNNNNTGC 2 cut(s) 270, 304
BciT130I CCWGG 3 cut(s) 606, 739, 1031
BcnI CCSGG 1 cut(s) 658
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 1 cut(s) 455
BfmI CTRYAG 4 cut(s) 40, 484, 581, 1044
BisI GCNGC 5 cut(s) 83, 276, 279, 282, 802
BlpI GCTNAGC 1 cut(s) 479
BlsI GCNGC 5 cut(s) 84, 277, 280, 283, 803
Bme1390I CCNGG 4 cut(s) 606, 658, 739, 1031
Bme18I GGWCC 2 cut(s) 103, 325
BmeRI GACNNNNNGTC 1 cut(s) 331
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 3 cut(s) 103, 325, 380
BmiI GGNNCC 5 cut(s) 295, 327, 382, 655, 948
BmrFI CCNGG 4 cut(s) 606, 658, 739, 1031
BmsI GCATC 2 cut(s) 307, 548
BpiI GAAGAC 1 cut(s) 849
BpmI CTGGAG 2 cut(s) 582, 1052
Bpu1102I GCTNAGC 1 cut(s) 479
Bpu14I TTCGAA 1 cut(s) 860
BpuEI CTTGAG 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 658
BsaAI YACGTR 1 cut(s) 884
BsaBI GATNNNNATC 1 cut(s) 681
BsaHI GRCGYC 1 cut(s) 245
BsaJI CCNNGG 2 cut(s) 99, 738
BsaWI WCCGGW 1 cut(s) 75
BsaXI ACNNNNNCTCC 8 cut(s) 53, 83, 134, 164, 338, 368, 821, 851
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 1036
Bse1I ACTGG 2 cut(s) 565, 641
Bse3DI GCAATG 3 cut(s) 215, 368, 384
Bse8I GATNNNNATC 1 cut(s) 681
BseBI CCWGG 3 cut(s) 606, 739, 1031
BseDI CCNNGG 2 cut(s) 99, 738
BseGI GGATG 3 cut(s) 403, 682, 919
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 2 cut(s) 78, 1036
BseMI GCAATG 3 cut(s) 215, 368, 384
BseMII CTCAG 7 cut(s) 36, 108, 465, 493, 635, 900, 975
BseNI ACTGG 2 cut(s) 565, 641
BseXI GCAGC 3 cut(s) 69, 293, 788
Bsh1236I CGCG 2 cut(s) 122, 226
BshFI GGCC 4 cut(s) 275, 381, 754, 1063
BsiHKAI GWGCWC 2 cut(s) 480, 960
BsiSI CCGG 2 cut(s) 76, 657
BslFI GGGAC 2 cut(s) 227, 250
BslI CCNNNNNNNGG 2 cut(s) 78, 1036
BsmAI GTCTC 1 cut(s) 252
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 2 cut(s) 227, 250
BsnI GGCC 4 cut(s) 275, 381, 754, 1063
Bsp119I TTCGAA 1 cut(s) 860
Bsp1286I GDGCHC 2 cut(s) 480, 960
Bsp143I GATC 5 cut(s) 117, 592, 660, 742, 900
Bsp1720I GCTNAGC 1 cut(s) 479
Bsp68I TCGCGA 1 cut(s) 226
BspACI CCGC 5 cut(s) 106, 122, 276, 279, 614
BspANI GGCC 4 cut(s) 275, 381, 754, 1063
BspCNI CTCAG 7 cut(s) 35, 107, 466, 492, 634, 901, 974
BspFNI CGCG 2 cut(s) 122, 226
BspLI GGNNCC 5 cut(s) 295, 327, 382, 655, 948
BspMAI CTGCAG 1 cut(s) 585
BspPI GGATC 3 cut(s) 600, 668, 750
BspT104I TTCGAA 1 cut(s) 860
BsrDI GCAATG 3 cut(s) 215, 368, 384
BsrI ACTGG 2 cut(s) 565, 641
BssECI CCNNGG 2 cut(s) 99, 738
BssMI GATC 5 cut(s) 117, 592, 660, 742, 900
BssNI GRCGYC 1 cut(s) 245
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 1 cut(s) 99
Bst2BI CACGAG 1 cut(s) 269
Bst2UI CCWGG 3 cut(s) 606, 739, 1031
Bst4CI ACNGT 3 cut(s) 430, 730, 986
Bst6I CTCTTC 1 cut(s) 151
BstACI GRCGYC 1 cut(s) 245
BstBAI YACGTR 1 cut(s) 884
BstBI TTCGAA 1 cut(s) 860
BstC8I GCNNGC 1 cut(s) 799
BstDEI CTNAG 8 cut(s) 22, 94, 474, 479, 621, 746, 909, 961
BstF5I GGATG 3 cut(s) 403, 682, 919
BstFNI CGCG 2 cut(s) 122, 226
BstKTI GATC 5 cut(s) 120, 595, 663, 745, 903
BstMAI GTCTC 1 cut(s) 252
BstMBI GATC 5 cut(s) 117, 592, 660, 742, 900
BstMWI GCNNNNNNNGC 5 cut(s) 119, 281, 355, 536, 760
BstNI CCWGG 3 cut(s) 606, 739, 1031
BstSCI CCNGG 4 cut(s) 604, 656, 737, 1029
BstSFI CTRYAG 4 cut(s) 40, 484, 581, 1044
BstUI CGCG 2 cut(s) 122, 226
BstV1I GCAGC 3 cut(s) 69, 293, 788
BstV2I GAAGAC 1 cut(s) 849
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
BsuRI GGCC 4 cut(s) 275, 381, 754, 1063
BtgZI GCGATG 1 cut(s) 525
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 3 cut(s) 403, 682, 919
BtsI GCAGTG 3 cut(s) 327, 511, 590
BtsIMutI CAGTG 4 cut(s) 327, 426, 511, 590
BtuMI TCGCGA 1 cut(s) 226
Cac8I GCNNGC 1 cut(s) 799
Cfr13I GGNCC 3 cut(s) 103, 325, 380
CseI GACGC 1 cut(s) 234
Csp6I GTAC 3 cut(s) 61, 665, 726
CviAII CATG 3 cut(s) 374, 810, 1065
CviQI GTAC 3 cut(s) 61, 665, 726
DdeI CTNAG 8 cut(s) 22, 94, 474, 479, 621, 746, 909, 961
DpnI GATC 5 cut(s) 119, 594, 662, 744, 902
DpnII GATC 5 cut(s) 117, 592, 660, 742, 900
DraIII CACNNNGTG 1 cut(s) 812
DriI GACNNNNNGTC 1 cut(s) 331
EaeI YGGCCR 2 cut(s) 273, 752
Eam1104I CTCTTC 1 cut(s) 151
Eam1105I GACNNNNNGTC 1 cut(s) 331
EarI CTCTTC 1 cut(s) 151
Ecl136II GAGCTC 2 cut(s) 478, 958
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 480, 960
Eco47I GGWCC 2 cut(s) 103, 325
Eco53kI GAGCTC 2 cut(s) 478, 958
EcoICRI GAGCTC 2 cut(s) 478, 958
EcoRII CCWGG 3 cut(s) 604, 737, 1029
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 480, 960
ErhI CCWWGG 1 cut(s) 99
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 3 cut(s) 377, 813, 1068
FaqI GGGAC 2 cut(s) 227, 250
FatI CATG 3 cut(s) 373, 809, 1064
Fnu4HI GCNGC 5 cut(s) 83, 276, 279, 282, 802
FokI GGATG 3 cut(s) 410, 669, 926
FriOI GRGCYC 2 cut(s) 480, 960
Fsp4HI GCNGC 5 cut(s) 83, 276, 279, 282, 802
FspBI CTAG 1 cut(s) 455
GluI GCNGC 5 cut(s) 83, 276, 279, 282, 802
GsuI CTGGAG 2 cut(s) 582, 1052
HaeIII GGCC 4 cut(s) 275, 381, 754, 1063
HapII CCGG 2 cut(s) 76, 657
HgaI GACGC 1 cut(s) 234
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 3 cut(s) 377, 813, 1068
HpaII CCGG 2 cut(s) 76, 657
HphI GGTGA 8 cut(s) 163, 177, 210, 304, 644, 798, 803, 953
Hpy166II GTNNAC 3 cut(s) 312, 325, 522
Hpy188I TCNGA 8 cut(s) 25, 136, 142, 346, 475, 597, 910, 1015
Hpy188III TCNNGA 3 cut(s) 225, 690, 1054
Hpy8I GTNNAC 3 cut(s) 312, 325, 522
Hpy99I CGWCG 1 cut(s) 337
HpyAV CCTTC 3 cut(s) 308, 339, 1020
HpyCH4III ACNGT 3 cut(s) 430, 730, 986
HpyCH4IV ACGT 3 cut(s) 303, 864, 883
HpyCH4V TGCA 4 cut(s) 320, 373, 583, 977
HpyF10VI GCNNNNNNNGC 5 cut(s) 119, 281, 355, 536, 760
HpyF3I CTNAG 8 cut(s) 22, 94, 474, 479, 621, 746, 909, 961
HpySE526I ACGT 3 cut(s) 303, 864, 883
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 3 cut(s) 377, 813, 1068
Kzo9I GATC 5 cut(s) 117, 592, 660, 742, 900
LmnI GCTCC 1 cut(s) 346
Lsp1109I GCAGC 3 cut(s) 69, 293, 788
LweI GCATC 2 cut(s) 307, 548
MaeI CTAG 1 cut(s) 455
MaeII ACGT 3 cut(s) 303, 864, 883
MaeIII GTNAC 2 cut(s) 424, 632
MalI GATC 5 cut(s) 119, 594, 662, 744, 902
MboI GATC 5 cut(s) 117, 592, 660, 742, 900
MboII GAAGA 4 cut(s) 138, 449, 849, 870
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 2 cut(s) 480, 960
MlsI TGGCCA 1 cut(s) 754
MluCI AATT 2 cut(s) 251, 918
MluNI TGGCCA 1 cut(s) 754
MlyI GAGTC 6 cut(s) 62, 125, 137, 197, 209, 479
Mox20I TGGCCA 1 cut(s) 754
MroXI GAANNNNTTC 2 cut(s) 652, 874
MscI TGGCCA 1 cut(s) 754
Msp20I TGGCCA 1 cut(s) 754
MspI CCGG 2 cut(s) 76, 657
MspR9I CCNGG 4 cut(s) 606, 658, 739, 1031
MvaI CCWGG 3 cut(s) 606, 739, 1031
MvnI CGCG 2 cut(s) 122, 226
MwoI GCNNNNNNNGC 5 cut(s) 119, 281, 355, 536, 760
NciI CCSGG 1 cut(s) 658
NdeII GATC 5 cut(s) 117, 592, 660, 742, 900
NlaIII CATG 3 cut(s) 377, 813, 1068
NlaIV GGNNCC 5 cut(s) 295, 327, 382, 655, 948
NmeAIII GCCGAG 1 cut(s) 570
NmuCI GTSAC 2 cut(s) 424, 632
NruI TCGCGA 1 cut(s) 226
NspV TTCGAA 1 cut(s) 860
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 350
PdmI GAANNNNTTC 2 cut(s) 652, 874
PfeI GAWTC 5 cut(s) 45, 137, 341, 640, 1040
PflFI GACNNNGTC 1 cut(s) 71
PfoI TCCNGGA 1 cut(s) 656
PkrI GCNGC 5 cut(s) 84, 277, 280, 283, 803
PleI GAGTC 6 cut(s) 62, 125, 137, 197, 209, 478
PpsI GAGTC 6 cut(s) 62, 125, 137, 197, 209, 478
Ppu21I YACGTR 1 cut(s) 884
Psp124BI GAGCTC 2 cut(s) 480, 960
Psp6I CCWGG 3 cut(s) 604, 737, 1029
PspGI CCWGG 3 cut(s) 604, 737, 1029
PspN4I GGNNCC 5 cut(s) 295, 327, 382, 655, 948
PspPI GGNCC 3 cut(s) 103, 325, 380
PstI CTGCAG 1 cut(s) 585
PsuI RGATCY 1 cut(s) 742
PsyI GACNNNGTC 1 cut(s) 71
RruI TCGCGA 1 cut(s) 226
RsaI GTAC 3 cut(s) 62, 666, 727
RsaNI GTAC 3 cut(s) 61, 665, 726
SacI GAGCTC 2 cut(s) 480, 960
SatI GCNGC 5 cut(s) 83, 276, 279, 282, 802
Sau3AI GATC 5 cut(s) 117, 592, 660, 742, 900
Sau96I GGNCC 3 cut(s) 103, 325, 380
SchI GAGTC 6 cut(s) 62, 125, 137, 197, 209, 479
ScrFI CCNGG 4 cut(s) 606, 658, 739, 1031
SduI GDGCHC 2 cut(s) 480, 960
SfaNI GCATC 2 cut(s) 307, 548
SfcI CTRYAG 4 cut(s) 40, 484, 581, 1044
SfuI TTCGAA 1 cut(s) 860
SinI GGWCC 2 cut(s) 103, 325
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 2 cut(s) 251, 918
SsiI CCGC 5 cut(s) 106, 122, 276, 279, 614
SspMI CTAG 1 cut(s) 455
SstI GAGCTC 2 cut(s) 480, 960
StyD4I CCNGG 4 cut(s) 604, 656, 737, 1029
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 3 cut(s) 430, 730, 986
TaiI ACGT 3 cut(s) 306, 867, 886
TaqI TCGA 3 cut(s) 129, 498, 860
TasI AATT 2 cut(s) 251, 918
TatI WGTACW 1 cut(s) 60
TauI GCSGC 2 cut(s) 278, 281
TfiI GAWTC 5 cut(s) 45, 137, 341, 640, 1040
TscAI CASTG 4 cut(s) 327, 433, 511, 590
TseFI GTSAC 2 cut(s) 424, 632
TseI GCWGC 3 cut(s) 82, 281, 801
Tsp45I GTSAC 2 cut(s) 424, 632
TspDTI ATGAA 1 cut(s) 671
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 4 cut(s) 327, 433, 511, 590
Tth111I GACNNNGTC 1 cut(s) 71
VpaK11BI GGWCC 2 cut(s) 103, 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmnI GAANNNNTTC 2 cut(s) 652, 874
XspI CTAG 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.