Rmu_sc0002316.1_g000052

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002316.1
OG0005514
Physical Location & Seq
Reverse (-)
247015 .. 247629
615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002316.1_g000052.1.cds

Sequence Viewer

Length: 345 bp
atgcttcagttcccagtctcagaagcttctgatgccatcttggcgcggcgcgtggcggctcgttggtggcgatctggaggagttacagaggagaagaagaagaagaagatgaagaacagtggaaacgagaagagtcacaaaggaaatgcgagagaggatcaggtgaagaaggacaggaaatcaggatcgagcatgaatggatcgcccaagaaaggtggccatggtggcaagttcacctgggccggtgatctgggctactttacagccgagatgggttttcagaaggaagtcatcgatgccaaggatccaaactttgaagacatagtcgagatcccaagcgtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

114

Amino Acids

12.62

Weight (kDa)

9.62

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 46, 51
AciI CCGC 2 cut(s) 46, 56
AclWI GGATC 6 cut(s) 165, 193, 208, 299, 312, 325
AcoI YGGCCR 1 cut(s) 217
AfiI CCNNNNNNNGG 1 cut(s) 212
AgsI TTSAA 1 cut(s) 317
AjnI CCWGG 1 cut(s) 236
AluBI AGCT 1 cut(s) 26
AluI AGCT 1 cut(s) 26
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 6 cut(s) 165, 193, 208, 299, 312, 325
AoxI GGCC 2 cut(s) 217, 240
AspLEI GCGC 2 cut(s) 46, 51
AspS9I GGNCC 1 cut(s) 240
AsuHPI GGTGA 3 cut(s) 175, 226, 257
BalI TGGCCA 1 cut(s) 219
BamHI GGATCC 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 324
BccI CCATC 2 cut(s) 44, 265
BciT130I CCWGG 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 22
BglI GCCNNNNNGGC 2 cut(s) 41, 225
BisI GCNGC 2 cut(s) 47, 57
BlsI GCNGC 2 cut(s) 48, 58
Bme1390I CCNGG 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 240
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 1 cut(s) 238
BmrI ACTGGG 1 cut(s) 8
BmsI GCATC 2 cut(s) 22, 286
BmuI ACTGGG 1 cut(s) 8
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 96
Bsa29I ATCGAT 1 cut(s) 294
BsaJI CCNNGG 3 cut(s) 220, 237, 300
Bsc4I CCNNNNNNNGG 1 cut(s) 212
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 238
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 3 cut(s) 220, 237, 300
BseLI CCNNNNNNNGG 1 cut(s) 212
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 2 cut(s) 93, 104
Bsh1236I CGCG 2 cut(s) 46, 51
BshFI GGCC 2 cut(s) 219, 242
BshVI ATCGAT 1 cut(s) 294
BsiSI CCGG 1 cut(s) 243
BslI CCNNNNNNNGG 1 cut(s) 212
BsmAI GTCTC 1 cut(s) 22
BsnI GGCC 2 cut(s) 219, 242
Bsp143I GATC 7 cut(s) 71, 157, 185, 200, 247, 304, 330
Bsp19I CCATGG 1 cut(s) 220
BspACI CCGC 2 cut(s) 46, 56
BspANI GGCC 2 cut(s) 219, 242
BspCNI CTCAG 1 cut(s) 32
BspDI ATCGAT 1 cut(s) 294
BspFNI CGCG 2 cut(s) 46, 51
BspLI GGNNCC 1 cut(s) 306
BspPI GGATC 6 cut(s) 165, 193, 208, 299, 312, 325
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 3 cut(s) 220, 237, 300
BssMI GATC 7 cut(s) 71, 157, 185, 200, 247, 304, 330
BssT1I CCWWGG 2 cut(s) 220, 300
Bst2UI CCWGG 1 cut(s) 238
Bst4CI ACNGT 1 cut(s) 119
Bst6I CTCTTC 1 cut(s) 125
BstDEI CTNAG 1 cut(s) 19
BstDSI CCRYGG 1 cut(s) 220
BstFNI CGCG 2 cut(s) 46, 51
BstHHI GCGC 2 cut(s) 46, 51
BstKTI GATC 7 cut(s) 74, 160, 188, 203, 250, 307, 333
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 7 cut(s) 71, 157, 185, 200, 247, 304, 330
BstMWI GCNNNNNNNGC 3 cut(s) 32, 41, 225
BstNI CCWGG 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 236
BstUI CGCG 2 cut(s) 46, 51
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 2 cut(s) 304, 330
BstYI RGATCY 2 cut(s) 304, 330
Bsu15I ATCGAT 1 cut(s) 294
BsuRI GGCC 2 cut(s) 219, 242
BsuTUI ATCGAT 1 cut(s) 294
BtgI CCRYGG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 124
CfoI GCGC 2 cut(s) 46, 51
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 1 cut(s) 240
ClaI ATCGAT 1 cut(s) 294
CseI GACGC 1 cut(s) 328
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviAII CATG 2 cut(s) 193, 221
CviJI RGCY 6 cut(s) 26, 59, 219, 242, 255, 266
CviKI_1 RGCY 6 cut(s) 26, 59, 219, 242, 255, 266
DdeI CTNAG 1 cut(s) 19
DpnI GATC 7 cut(s) 73, 159, 187, 202, 249, 306, 332
DpnII GATC 7 cut(s) 71, 157, 185, 200, 247, 304, 330
EaeI YGGCCR 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Eco130I CCWWGG 2 cut(s) 220, 300
EcoRII CCWGG 1 cut(s) 236
EcoT14I CCWWGG 2 cut(s) 220, 300
ErhI CCWWGG 2 cut(s) 220, 300
FaeI CATG 2 cut(s) 196, 224
FaiI YATR 3 cut(s) 194, 222, 323
FatI CATG 2 cut(s) 192, 220
Fnu4HI GCNGC 2 cut(s) 47, 57
Fsp4HI GCNGC 2 cut(s) 47, 57
GlaI GCGC 2 cut(s) 45, 50
GluI GCNGC 2 cut(s) 47, 57
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 2 cut(s) 219, 242
HapII CCGG 1 cut(s) 243
HgaI GACGC 1 cut(s) 328
HhaI GCGC 2 cut(s) 46, 51
Hin1II CATG 2 cut(s) 196, 224
Hin6I GCGC 2 cut(s) 44, 49
HinP1I GCGC 2 cut(s) 44, 49
HindIII AAGCTT 1 cut(s) 24
HinfI GANTC 1 cut(s) 133
HpaII CCGG 1 cut(s) 243
HphI GGTGA 3 cut(s) 175, 226, 257
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 4 cut(s) 22, 31, 282, 344
Hpy188III TCNNGA 3 cut(s) 75, 183, 328
Hpy8I GTNNAC 1 cut(s) 234
HpyAV CCTTC 2 cut(s) 163, 277
HpyCH4III ACNGT 1 cut(s) 119
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 41, 225
HpyF3I CTNAG 1 cut(s) 19
Hsp92II CATG 2 cut(s) 196, 224
HspAI GCGC 2 cut(s) 44, 49
Kzo9I GATC 7 cut(s) 71, 157, 185, 200, 247, 304, 330
LpnPI CCDG 9 cut(s) 27, 60, 146, 160, 168, 223, 236, 250, 256
LweI GCATC 2 cut(s) 22, 286
MaeIII GTNAC 2 cut(s) 82, 134
MalI GATC 7 cut(s) 73, 159, 187, 202, 249, 306, 332
MboI GATC 7 cut(s) 71, 157, 185, 200, 247, 304, 330
MboII GAAGA 9 cut(s) 106, 109, 112, 115, 118, 124, 142, 178, 329
MflI RGATCY 2 cut(s) 304, 330
MlsI TGGCCA 1 cut(s) 219
MluNI TGGCCA 1 cut(s) 219
MlyI GAGTC 1 cut(s) 142
MnlI CCTC 3 cut(s) 71, 82, 148
Mox20I TGGCCA 1 cut(s) 219
MscI TGGCCA 1 cut(s) 219
Msp20I TGGCCA 1 cut(s) 219
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 238
MteI GCGCNGCGC 1 cut(s) 47
MvaI CCWGG 1 cut(s) 238
MvnI CGCG 2 cut(s) 46, 51
MwoI GCNNNNNNNGC 3 cut(s) 32, 41, 225
NcoI CCATGG 1 cut(s) 220
NdeII GATC 7 cut(s) 71, 157, 185, 200, 247, 304, 330
NlaIII CATG 2 cut(s) 196, 224
NlaIV GGNNCC 1 cut(s) 306
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 67
PflFI GACNNNGTC 1 cut(s) 323
PkrI GCNGC 2 cut(s) 48, 58
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Psp6I CCWGG 1 cut(s) 236
PspGI CCWGG 1 cut(s) 236
PspN4I GGNNCC 1 cut(s) 306
PspPI GGNCC 1 cut(s) 240
PsuI RGATCY 2 cut(s) 304, 330
PsyI GACNNNGTC 1 cut(s) 323
SatI GCNGC 2 cut(s) 47, 57
Sau3AI GATC 7 cut(s) 71, 157, 185, 200, 247, 304, 330
Sau96I GGNCC 1 cut(s) 240
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 238
SetI ASST 4 cut(s) 28, 165, 217, 239
SfaNI GCATC 2 cut(s) 22, 286
SsiI CCGC 2 cut(s) 46, 56
StyD4I CCNGG 1 cut(s) 236
StyI CCWWGG 2 cut(s) 220, 300
TaaI ACNGT 1 cut(s) 119
TaqI TCGA 3 cut(s) 188, 294, 327
TauI GCSGC 2 cut(s) 49, 59
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 2 cut(s) 125, 209
TspRI CASTG 1 cut(s) 124
Tth111I GACNNNGTC 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.