Rorug02G0022900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005514
Physical Location & Seq
Reverse (-)
1824537 .. 1825109
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0022900.1

Sequence Viewer

Length: 573 bp
ATGGAGGGAAGACCAGTCGATTGTAATATTGAAGCGGCTGATGATATTACGTGCAAACTGGGGCAGATTATCCCTCCATTCATGAATCTAGTCACTGAGATAAATGTGGCAGATGATGTCTGTTTTATCTTATTTGTGGAAAAATCTCCTACTTTTCTAGAACTGACACTAGAAAACTTCCACAATAAATATCATTGTATCATTATTACCGGAACGGGCCACGCTGATGTTGTATCCAGACAACTCACGAAAATACTAATGGATGATTTCAAGCTACCAGCGTTCTGCCTTGTAGATTGTGATCCATGGGGCATTGAGATTTCAGCTATTATCAGGCACGGCTCGGTGAATATGGCGTATGACAGTAAAAATTTAGCCAGCCCCTTGATGTATTGGTTAGGTATTTGGCCAAGTGATTTGGAAAATCTTAATATACCTTCAGAGACTTTCACTAAGAAAGATTTGAAGAAATTAGAGGATTTGATGGAGAAAGAATTTTTTGATGACGATAAGACGTTGAAAAAGGAGCTGAATTATATGATTCGATCTACACCAAAAGAAGGTAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

190

Amino Acids

21.69

Weight (kDa)

4.78

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOP6A-Spo11_Toprim PF21180 42 - 186 1.2e-43 Topoisomerase 6 subunit A/Spo11, Toprim domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 35
AclWI GGATC 1 cut(s) 296
AcoI YGGCCR 1 cut(s) 407
AcsI RAATTY 2 cut(s) 370, 494
AcuI CTGAAG 1 cut(s) 423
AfiI CCNNNNNNNGG 1 cut(s) 560
AgsI TTSAA 4 cut(s) 32, 271, 466, 520
AluBI AGCT 4 cut(s) 274, 326, 529, 569
AluI AGCT 4 cut(s) 274, 326, 529, 569
Alw26I GTCTC 1 cut(s) 437
AlwI GGATC 1 cut(s) 296
AoxI GGCC 2 cut(s) 217, 407
ApoI RAATTY 2 cut(s) 370, 494
AspS9I GGNCC 1 cut(s) 217
AsuHPI GGTGA 1 cut(s) 358
BalI TGGCCA 1 cut(s) 409
BbsI GAAGAC 1 cut(s) 16
BccI CCATC 1 cut(s) 478
BceAI ACGGC 1 cut(s) 355
BciVI GTATCC 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 3 cut(s) 89, 158, 170
BfuI GTATCC 1 cut(s) 244
BisI GCNGC 1 cut(s) 36
BlsI GCNGC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 217
BmrI ACTGGG 1 cut(s) 68
BmuI ACTGGG 1 cut(s) 68
BpiI GAAGAC 1 cut(s) 16
BsaAI YACGTR 1 cut(s) 51
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 305
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 1 cut(s) 560
Bse1I ACTGG 2 cut(s) 14, 63
Bse8I GATNNNNATC 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 305
BseGI GGATG 1 cut(s) 268
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 560
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 2 cut(s) 14, 63
BshFI GGCC 2 cut(s) 219, 409
BsiSI CCGG 1 cut(s) 210
BslI CCNNNNNNNGG 1 cut(s) 560
BsmAI GTCTC 1 cut(s) 437
BsnI GGCC 2 cut(s) 219, 409
Bsp143I GATC 2 cut(s) 301, 545
Bsp19I CCATGG 1 cut(s) 305
BspACI CCGC 1 cut(s) 35
BspANI GGCC 2 cut(s) 219, 409
BspCNI CTCAG 1 cut(s) 88
BspHI TCATGA 1 cut(s) 81
BspPI GGATC 1 cut(s) 296
BsrI ACTGG 2 cut(s) 14, 63
BssECI CCNNGG 1 cut(s) 305
BssMI GATC 2 cut(s) 301, 545
BssT1I CCWWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 365
BstBAI YACGTR 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 379
BstDEI CTNAG 2 cut(s) 96, 453
BstDSI CCRYGG 1 cut(s) 305
BstF5I GGATG 1 cut(s) 268
BstKTI GATC 2 cut(s) 304, 548
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 2 cut(s) 301, 545
BstV2I GAAGAC 1 cut(s) 16
BsuI GTATCC 1 cut(s) 244
BsuRI GGCC 2 cut(s) 219, 409
BtgI CCRYGG 1 cut(s) 305
BtsCI GGATG 1 cut(s) 268
BtsIMutI CAGTG 1 cut(s) 93
Cac8I GCNNGC 1 cut(s) 379
CciI TCATGA 1 cut(s) 81
Cfr13I GGNCC 1 cut(s) 217
CviAII CATG 2 cut(s) 82, 306
DdeI CTNAG 2 cut(s) 96, 453
DpnI GATC 2 cut(s) 303, 547
DpnII GATC 2 cut(s) 301, 545
EaeI YGGCCR 1 cut(s) 407
Eco130I CCWWGG 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 2 cut(s) 85, 309
FaiI YATR 7 cut(s) 83, 307, 353, 360, 434, 537, 539
FatI CATG 2 cut(s) 81, 305
Fnu4HI GCNGC 1 cut(s) 36
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 1 cut(s) 36
FspBI CTAG 3 cut(s) 89, 158, 170
GluI GCNGC 1 cut(s) 36
HaeIII GGCC 2 cut(s) 219, 409
HapII CCGG 1 cut(s) 210
Hin1II CATG 2 cut(s) 85, 309
HinfI GANTC 2 cut(s) 85, 541
HpaII CCGG 1 cut(s) 210
HphI GGTGA 1 cut(s) 358
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 4 cut(s) 82, 158, 237, 247
HpyAV CCTTC 2 cut(s) 447, 554
HpyCH4III ACNGT 1 cut(s) 365
HpyCH4IV ACGT 2 cut(s) 50, 515
HpyCH4V TGCA 1 cut(s) 54
HpyF3I CTNAG 2 cut(s) 96, 453
HpySE526I ACGT 2 cut(s) 50, 515
Hsp92II CATG 2 cut(s) 85, 309
Kzo9I GATC 2 cut(s) 301, 545
LmnI GCTCC 1 cut(s) 526
LpnPI CCDG 7 cut(s) 27, 44, 223, 250, 291, 319, 391
MaeI CTAG 3 cut(s) 89, 158, 170
MaeII ACGT 2 cut(s) 50, 515
MaeIII GTNAC 1 cut(s) 91
MalI GATC 2 cut(s) 303, 547
MboI GATC 2 cut(s) 301, 545
MboII GAAGA 2 cut(s) 21, 478
MlsI TGGCCA 1 cut(s) 409
MluCI AATT 4 cut(s) 370, 470, 494, 532
MluNI TGGCCA 1 cut(s) 409
MnlI CCTC 2 cut(s) 84, 469
Mox20I TGGCCA 1 cut(s) 409
MscI TGGCCA 1 cut(s) 409
MseI TTAA 1 cut(s) 429
MslI CAYNNNNRTG 1 cut(s) 225
Msp20I TGGCCA 1 cut(s) 409
MspI CCGG 1 cut(s) 210
NcoI CCATGG 1 cut(s) 305
NdeII GATC 2 cut(s) 301, 545
NlaIII CATG 2 cut(s) 85, 309
NmuCI GTSAC 1 cut(s) 91
PagI TCATGA 1 cut(s) 81
PfeI GAWTC 2 cut(s) 85, 541
PkrI GCNGC 1 cut(s) 37
Ppu21I YACGTR 1 cut(s) 51
PspPI GGNCC 1 cut(s) 217
RseI CAYNNNNRTG 1 cut(s) 225
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 1 cut(s) 36
Sau3AI GATC 2 cut(s) 301, 545
Sau96I GGNCC 1 cut(s) 217
SetI ASST 9 cut(s) 53, 276, 328, 403, 439, 518, 531, 565, 571
SmiMI CAYNNNNRTG 1 cut(s) 225
Sse9I AATT 4 cut(s) 370, 470, 494, 532
SsiI CCGC 1 cut(s) 35
SspI AATATT 1 cut(s) 28
SspMI CTAG 3 cut(s) 89, 158, 170
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 1 cut(s) 365
TaiI ACGT 2 cut(s) 53, 518
TaqI TCGA 2 cut(s) 18, 544
TasI AATT 4 cut(s) 370, 470, 494, 532
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 2 cut(s) 85, 541
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TscAI CASTG 1 cut(s) 100
TseFI GTSAC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 2 cut(s) 70, 98
TspRI CASTG 1 cut(s) 100
XapI RAATTY 2 cut(s) 370, 494
XbaI TCTAGA 1 cut(s) 157
XspI CTAG 3 cut(s) 89, 158, 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.