MD13G1194100.v1.1

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012372
Physical Location & Seq
Reverse (-)
16800148 .. 16802441
2294 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1194100.v1.1.491

Sequence Viewer

Length: 423 bp
ATGGCGGCAATGGCAACAGGAGTGAGTCTCAACCACATTTCGAGAGAATCCTCCGACATTCGGCGTCTCGCCGATTTCTACAAAGAGATATTTGGGCTCGAGGAGATTGAGAGCCCAAACTTCGGGGAGTTCAAGGTGATATGGCTCAAGGCGCCTGGGAATTTCGCTCTCCACCTGATCGAAAGAAACCCTAGTTTTAACCTTCCGGAAGGACCATGGAGCGCTACGTCACCGGTTGCTGACCCGAGCCACCTCCCCAGAGGCCACCACATCTGCTTCAACGTCTCCAATTTCCAGTCTTTTGTGAAAACCCTCAAGGAAAAGGGAATCCAGACGTTTGAGAGGTCCTTACCTGATGGTAAGGTCAAGCAAGTCTTCTTCTTTGATCCAGATGGCAATGGATTGGAGGTTGCAGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_4

Protein Analysis

141

Amino Acids

15.65

Weight (kDa)

5.93

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 10 - 137 2.1e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 12 - 121 5.4e-08 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012372)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32090 AT2G32090
fragaria_vesca FvH4_4g16340 FvH4_4g16340
malus_domestica MD13G1194100.v1.1
prunus_persica Prupe.1G014900_v2.0.a1 Prupe.1G014900_v2.0.a1
pyrus_communis pycom13g16640
rosa_chinensis RchiOBHm_Chr4g0416051
rosa_laevigata RLG00000008022
rosa_multiflora Rmu_sc0007573.1_g000005 Rmu_sc0007573.1_g000007
rosa_roxburghii Rroxscaffold_5G00359880
rosa_rugosa Rorug04G0137700
rosa_samantha Rh4AG200000 Rh4BG197600 Rh4CG211300 Rh4DG197800
rosa_wichuraiana Rw4G016980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 404
AccB1I GGYRCC 1 cut(s) 151
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 380
AcsI RAATTY 1 cut(s) 160
AcyI GRCGYC 2 cut(s) 64, 152
AfeI AGCGCT 1 cut(s) 223
AfiI CCNNNNNNNGG 2 cut(s) 60, 122
AgeI ACCGGT 1 cut(s) 232
AgsI TTSAA 2 cut(s) 133, 280
AjnI CCWGG 1 cut(s) 154
Alw26I GTCTC 3 cut(s) 32, 71, 289
AlwI GGATC 1 cut(s) 380
Ama87I CYCGRG 2 cut(s) 98, 244
Aor13HI TCCGGA 1 cut(s) 205
Aor51HI AGCGCT 1 cut(s) 223
AoxI GGCC 1 cut(s) 262
ApoI RAATTY 1 cut(s) 160
AsiGI ACCGGT 1 cut(s) 232
AspLEI GCGC 2 cut(s) 154, 224
AspS9I GGNCC 2 cut(s) 212, 345
AsuHPI GGTGA 2 cut(s) 148, 222
AvaI CYCGRG 2 cut(s) 98, 244
AvaII GGWCC 2 cut(s) 212, 345
BanI GGYRCC 1 cut(s) 151
BanII GRGCYC 2 cut(s) 99, 116
BbsI GAAGAC 1 cut(s) 367
BccI CCATC 2 cut(s) 350, 386
BciT130I CCWGG 1 cut(s) 156
BcoDI GTCTC 3 cut(s) 32, 71, 289
BfaI CTAG 1 cut(s) 192
BfoI RGCGCY 2 cut(s) 155, 225
BfuAI ACCTGC 1 cut(s) 404
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 156
Bme18I GGWCC 2 cut(s) 212, 345
BmeT110I CYCGRG 2 cut(s) 98, 244
BmgT120I GGNCC 2 cut(s) 212, 345
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 1 cut(s) 156
BpiI GAAGAC 1 cut(s) 367
BplI GAGNNNNNCTC 2 cut(s) 12, 44
BpuEI CTTGAG 2 cut(s) 131, 299
BsaHI GRCGYC 2 cut(s) 64, 152
BsaJI CCNNGG 2 cut(s) 155, 215
BsaWI WCCGGW 2 cut(s) 205, 232
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 122
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 1 cut(s) 295
Bse3DI GCAATG 2 cut(s) 15, 403
BseAI TCCGGA 1 cut(s) 205
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 155, 215
BseLI CCNNNNNNNGG 2 cut(s) 60, 122
BseMI GCAATG 2 cut(s) 15, 403
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 1 cut(s) 116
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 1 cut(s) 151
BshTI ACCGGT 1 cut(s) 232
BsiHKCI CYCGRG 2 cut(s) 98, 244
BsiSI CCGG 2 cut(s) 206, 233
BslI CCNNNNNNNGG 2 cut(s) 60, 122
BsmAI GTCTC 3 cut(s) 32, 71, 289
BsmBI CGTCTC 2 cut(s) 71, 289
BsnI GGCC 1 cut(s) 264
BsoBI CYCGRG 2 cut(s) 98, 244
Bsp1286I GDGCHC 2 cut(s) 99, 116
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 2 cut(s) 177, 385
Bsp19I CCATGG 1 cut(s) 215
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 264
BspEI TCCGGA 1 cut(s) 205
BspLI GGNNCC 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 404
BspPI GGATC 1 cut(s) 380
BspT107I GGYRCC 1 cut(s) 151
BsrDI GCAATG 2 cut(s) 15, 403
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 1 cut(s) 295
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 2 cut(s) 155, 215
BssMI GATC 2 cut(s) 177, 385
BssNI GRCGYC 2 cut(s) 64, 152
BssT1I CCWWGG 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 156
BstACI GRCGYC 2 cut(s) 64, 152
BstDSI CCRYGG 1 cut(s) 215
BstH2I RGCGCY 2 cut(s) 155, 225
BstHHI GCGC 2 cut(s) 154, 224
BstKTI GATC 2 cut(s) 180, 388
BstMAI GTCTC 3 cut(s) 32, 71, 289
BstMBI GATC 2 cut(s) 177, 385
BstMWI GCNNNNNNNGC 2 cut(s) 11, 151
BstNI CCWGG 1 cut(s) 156
BstSCI CCNGG 1 cut(s) 154
BstV2I GAAGAC 1 cut(s) 367
BsuRI GGCC 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 215
BveI ACCTGC 1 cut(s) 404
CfoI GCGC 2 cut(s) 154, 224
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 2 cut(s) 212, 345
CseI GACGC 1 cut(s) 53
CspAI ACCGGT 1 cut(s) 232
CviAII CATG 1 cut(s) 216
CviJI RGCY 5 cut(s) 97, 114, 145, 249, 264
CviKI_1 RGCY 5 cut(s) 97, 114, 145, 249, 264
DinI GGCGCC 1 cut(s) 153
DpnI GATC 2 cut(s) 179, 387
DpnII GATC 2 cut(s) 177, 385
Eco130I CCWWGG 1 cut(s) 215
Eco24I GRGCYC 2 cut(s) 99, 116
Eco47I GGWCC 2 cut(s) 212, 345
Eco47III AGCGCT 1 cut(s) 223
Eco88I CYCGRG 2 cut(s) 98, 244
EcoO109I RGGNCCY 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 215
EcoT38I GRGCYC 2 cut(s) 99, 116
EgeI GGCGCC 1 cut(s) 153
EheI GGCGCC 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 215
Esp3I CGTCTC 2 cut(s) 71, 289
FaeI CATG 1 cut(s) 219
FaiI YATR 2 cut(s) 142, 217
FalI AAGNNNNNCTT 2 cut(s) 359, 391
FatI CATG 1 cut(s) 215
Fnu4HI GCNGC 1 cut(s) 6
FriOI GRGCYC 2 cut(s) 99, 116
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 192
GlaI GCGC 2 cut(s) 153, 223
GluI GCNGC 1 cut(s) 6
HaeII RGCGCY 2 cut(s) 155, 225
HaeIII GGCC 1 cut(s) 264
HapII CCGG 2 cut(s) 206, 233
HgaI GACGC 1 cut(s) 53
HhaI GCGC 2 cut(s) 154, 224
Hin1I GRCGYC 2 cut(s) 64, 152
Hin1II CATG 1 cut(s) 219
Hin6I GCGC 2 cut(s) 152, 222
HinP1I GCGC 2 cut(s) 152, 222
HinfI GANTC 3 cut(s) 25, 47, 327
HpaII CCGG 2 cut(s) 206, 233
HphI GGTGA 2 cut(s) 148, 222
Hpy188I TCNGA 1 cut(s) 55
Hpy188III TCNNGA 4 cut(s) 42, 206, 331, 389
HpyAV CCTTC 2 cut(s) 203, 212
HpyCH4IV ACGT 3 cut(s) 227, 282, 335
HpyCH4V TGCA 1 cut(s) 413
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 151
HpySE526I ACGT 3 cut(s) 227, 282, 335
Hsp92I GRCGYC 2 cut(s) 64, 152
Hsp92II CATG 1 cut(s) 219
HspAI GCGC 2 cut(s) 152, 222
KasI GGCGCC 1 cut(s) 151
Kpn2I TCCGGA 1 cut(s) 205
Kzo9I GATC 2 cut(s) 177, 385
LmnI GCTCC 1 cut(s) 219
MaeI CTAG 1 cut(s) 192
MaeII ACGT 3 cut(s) 227, 282, 335
MaeIII GTNAC 1 cut(s) 228
MalI GATC 2 cut(s) 179, 387
MboI GATC 2 cut(s) 177, 385
MboII GAAGA 2 cut(s) 367, 370
MhlI GDGCHC 2 cut(s) 99, 116
MluCI AATT 2 cut(s) 160, 289
Mly113I GGCGCC 1 cut(s) 152
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 7 cut(s) 61, 94, 254, 263, 323, 336, 400
MroI TCCGGA 1 cut(s) 205
MseI TTAA 1 cut(s) 198
MspI CCGG 2 cut(s) 206, 233
MspR9I CCNGG 1 cut(s) 156
MvaI CCWGG 1 cut(s) 156
MwoI GCNNNNNNNGC 2 cut(s) 11, 151
NarI GGCGCC 1 cut(s) 152
NcoI CCATGG 1 cut(s) 215
NdeII GATC 2 cut(s) 177, 385
NlaIII CATG 1 cut(s) 219
NlaIV GGNNCC 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 228
PaeR7I CTCGAG 1 cut(s) 98
PfeI GAWTC 2 cut(s) 47, 327
PinAI ACCGGT 1 cut(s) 232
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 33
PluTI GGCGCC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 33
PpuMI RGGWCCY 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 212, 345
PspPPI RGGWCCY 1 cut(s) 345
PspXI VCTCGAGB 1 cut(s) 98
SaqAI TTAA 1 cut(s) 198
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 2 cut(s) 177, 385
Sau96I GGNCC 2 cut(s) 212, 345
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 156
SduI GDGCHC 2 cut(s) 99, 116
SfoI GGCGCC 1 cut(s) 153
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 2 cut(s) 212, 345
SlaI CTCGAG 1 cut(s) 98
SmlI CTYRAG 3 cut(s) 98, 146, 314
SmoI CTYRAG 3 cut(s) 98, 146, 314
Sse9I AATT 2 cut(s) 160, 289
SsiI CCGC 1 cut(s) 5
SspDI GGCGCC 1 cut(s) 151
SspMI CTAG 1 cut(s) 192
StyD4I CCNGG 1 cut(s) 154
StyI CCWWGG 1 cut(s) 215
TaiI ACGT 3 cut(s) 230, 285, 338
TaqI TCGA 3 cut(s) 41, 99, 180
TasI AATT 2 cut(s) 160, 289
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 47, 327
Tru1I TTAA 1 cut(s) 198
Tru9I TTAA 1 cut(s) 198
TseFI GTSAC 1 cut(s) 228
Tsp45I GTSAC 1 cut(s) 228
VpaK11BI GGWCC 2 cut(s) 212, 345
XapI RAATTY 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 98
XspI CTAG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.