FvH4_4g16930

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013137
Physical Location & Seq
Reverse (-)
20870096 .. 20873030
2935 bp
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UTR
Exon/CDS
Intron
FvH4_4g16930.t1

Sequence Viewer

Length: 321 bp
ATGGCGAGCGCGGTGGACGCAGCAGGAGATCCGATCCCGACGTCGGCGGTGCTGACGTCGGCGGCGAAGCACATTCAATTCAGGTGCCAAGAGGAGAACGTGGCCTTTCTCAAGTGCAAGAAGAAGGACGCTAATCCCGAGAAGTGTCTCGACCAAGGTCGCCAAGTCACTCGCTGCGTCCTTTCCTTGCTGAAAGATCTTCATCAGAGGTGCACCAAAGAGATGGACGAGTATGTCGGATGCATGTATTACAATACAAATGAATTTGATTTATGTCGCAAAGAGCAGGAGAAATTTGAGAAAAAATGCCCATTGGAATGA

Protein Analysis

107

Amino Acids

12.12

Weight (kDa)

6.72

Isoelectric Point (pI)

31.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHCH PF06747 71 - 103 1.9e-07 CHCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18800 AT5G18800
fragaria_vesca FvH4_4g16930
malus_domestica MD05G1041600.v1.1 MD10G1048000.v1.1 MD13G1175600.v1.1 MD16G1177400.v1.1
prunus_persica Prupe.1G144300_v2.0.a1
pyrus_communis pycom05g03430 pycom10g03400
rosa_chinensis RchiOBHm_Chr4g0420511
rosa_laevigata RLG00000007755
rosa_multiflora Rmu_sc0002248.1_g000050
rosa_roxburghii Rroxscaffold_5G00363130
rosa_rugosa Rorug04G0164700 Rorug04G0164700
rosa_samantha Rh4BG228800
rosa_wichuraiana Rw4G019520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AatII GACGTC 2 cut(s) 44, 59
AccB1I GGYRCC 1 cut(s) 84
AccII CGCG 1 cut(s) 11
AciI CCGC 3 cut(s) 11, 47, 62
AclWI GGATC 2 cut(s) 23, 28
AcsI RAATTY 2 cut(s) 263, 293
AcyI GRCGYC 2 cut(s) 41, 56
AfiI CCNNNNNNNGG 1 cut(s) 43
AgsI TTSAA 1 cut(s) 77
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 1 cut(s) 152
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 2 cut(s) 23, 28
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 1 cut(s) 102
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 2 cut(s) 20, 174
ApoI RAATTY 2 cut(s) 263, 293
AspLEI GCGC 1 cut(s) 11
AvaI CYCGRG 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 215
BanI GGYRCC 1 cut(s) 84
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 2 cut(s) 32, 161
BccI CCATC 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 152
BglII AGATCT 1 cut(s) 196
BisI GCNGC 3 cut(s) 21, 63, 175
BlsI GCNGC 3 cut(s) 22, 64, 176
BmeT110I CYCGRG 1 cut(s) 137
BmiI GGNNCC 1 cut(s) 86
BmsI GCATC 1 cut(s) 230
BoxI GACNNNNGTC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 201
BsaHI GRCGYC 2 cut(s) 41, 56
BsaJI CCNNGG 1 cut(s) 154
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 201
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 1 cut(s) 245
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 43
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 2 cut(s) 32, 161
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 1 cut(s) 104
BshNI GGYRCC 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 215
BsiHKCI CYCGRG 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 152
BsnI GGCC 1 cut(s) 104
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 3 cut(s) 28, 33, 196
BspACI CCGC 3 cut(s) 11, 47, 62
BspANI GGCC 1 cut(s) 104
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 86
BspPI GGATC 2 cut(s) 23, 28
BspT107I GGYRCC 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 3 cut(s) 28, 33, 196
BssNI GRCGYC 2 cut(s) 41, 56
BssT1I CCWWGG 1 cut(s) 154
BstACI GRCGYC 2 cut(s) 41, 56
BstC8I GCNNGC 1 cut(s) 7
BstF5I GGATG 1 cut(s) 245
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 3 cut(s) 31, 36, 199
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 3 cut(s) 28, 33, 196
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 247
BstPAI GACNNNNGTC 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 32, 161
BstX2I RGATCY 2 cut(s) 28, 196
BstXI CCANNNNNNTGG 1 cut(s) 223
BstYI RGATCY 2 cut(s) 28, 196
BsuRI GGCC 1 cut(s) 104
BtsCI GGATG 1 cut(s) 245
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 11
CseI GACGC 3 cut(s) 26, 137, 166
CviAII CATG 1 cut(s) 244
CviJI RGCY 1 cut(s) 104
CviKI_1 RGCY 1 cut(s) 104
DpnI GATC 3 cut(s) 30, 35, 198
DpnII GATC 3 cut(s) 28, 33, 196
DrdI GACNNNNNNGTC 1 cut(s) 233
DseDI GACNNNNNNGTC 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 154
Eco88I CYCGRG 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 154
EcoT22I ATGCAT 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 1 cut(s) 247
FaiI YATR 3 cut(s) 234, 245, 274
FatI CATG 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 21, 63, 175
FokI GGATG 1 cut(s) 252
Fsp4HI GCNGC 3 cut(s) 21, 63, 175
GlaI GCGC 1 cut(s) 10
GluI GCNGC 3 cut(s) 21, 63, 175
HaeIII GGCC 1 cut(s) 104
HgaI GACGC 3 cut(s) 26, 137, 166
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 2 cut(s) 41, 56
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
Hpy166II GTNNAC 2 cut(s) 16, 213
Hpy188I TCNGA 3 cut(s) 33, 207, 239
Hpy188III TCNNGA 3 cut(s) 37, 137, 149
Hpy8I GTNNAC 2 cut(s) 16, 213
Hpy99I CGWCG 3 cut(s) 43, 46, 61
HpyAV CCTTC 1 cut(s) 118
HpyCH4IV ACGT 3 cut(s) 41, 56, 99
HpyCH4V TGCA 3 cut(s) 117, 213, 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpySE526I ACGT 3 cut(s) 41, 56, 99
Hsp92I GRCGYC 2 cut(s) 41, 56
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 1 cut(s) 9
Kzo9I GATC 3 cut(s) 28, 33, 196
LpnPI CCDG 3 cut(s) 9, 67, 272
Lsp1109I GCAGC 2 cut(s) 32, 161
LweI GCATC 1 cut(s) 230
MaeII ACGT 3 cut(s) 41, 56, 99
MaeIII GTNAC 1 cut(s) 166
MalI GATC 3 cut(s) 30, 35, 198
MboI GATC 3 cut(s) 28, 33, 196
MboII GAAGA 2 cut(s) 133, 191
MflI RGATCY 2 cut(s) 28, 196
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 3 cut(s) 77, 263, 293
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 2 cut(s) 85, 201
Mph1103I ATGCAT 1 cut(s) 245
MslI CAYNNNNRTG 1 cut(s) 316
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 17
NdeII GATC 3 cut(s) 28, 33, 196
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 1 cut(s) 86
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 1 cut(s) 247
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 135
PkrI GCNGC 3 cut(s) 22, 64, 176
PshAI GACNNNNGTC 1 cut(s) 156
PspN4I GGNNCC 1 cut(s) 86
PsuI RGATCY 2 cut(s) 28, 196
RseI CAYNNNNRTG 1 cut(s) 316
SatI GCNGC 3 cut(s) 21, 63, 175
Sau3AI GATC 3 cut(s) 28, 33, 196
SduI GDGCHC 1 cut(s) 215
SetI ASST 6 cut(s) 44, 59, 86, 102, 160, 212
SfaNI GCATC 1 cut(s) 230
SmiMI CAYNNNNRTG 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 3 cut(s) 77, 263, 293
SsiI CCGC 3 cut(s) 11, 47, 62
StyI CCWWGG 1 cut(s) 154
TaiI ACGT 3 cut(s) 44, 59, 102
TaqI TCGA 1 cut(s) 150
TasI AATT 3 cut(s) 77, 263, 293
TauI GCSGC 1 cut(s) 65
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 2 cut(s) 20, 174
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 191, 276
VneI GTGCAC 1 cut(s) 211
XapI RAATTY 2 cut(s) 263, 293
XceI RCATGY 1 cut(s) 247
ZraI GACGTC 2 cut(s) 42, 57
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.