RchiOBHm_Chr4g0420511

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0013137
Physical Location & Seq
Reverse (-)
46301675 .. 46304018
2344 bp
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UTR
Exon/CDS
Intron
PRQ39025

Sequence Viewer

Length: 321 bp
ATGGCGAGCGCGGTGGACGCAGCAGGAGATCCGATCCCGACGTCGGCGGTGCTGACGGCGGCGGCGAAGCACATTCAGTTCAGATGCCAAGAGGAGAACGTGGCCTTCCTCAAGTGCAAGAAGAAGGACCCCAATCCCGAGAAGTGTCTCGACCAAGGTCGCCAAGTCACTCGCTGCGTCCTCACCTTGCTGAAAGATCTTCATCAGAGGTGCACGAAAGAGATGGATGAGTACGTTGGATGCATGTATTACAATACAAATGAATTTGATTTATGTCGCAAAGAGCAGGAGGAATTCGAGAAAAAATGCCCATTGGAATGA

Protein Analysis

106

Amino Acids

12.15

Weight (kDa)

5.73

Isoelectric Point (pI)

34.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHCH PF06747 71 - 103 1.4e-07 CHCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18800 AT5G18800
fragaria_vesca FvH4_4g16930
malus_domestica MD05G1041600.v1.1 MD10G1048000.v1.1 MD13G1175600.v1.1 MD16G1177400.v1.1
prunus_persica Prupe.1G144300_v2.0.a1
pyrus_communis pycom05g03430 pycom10g03400
rosa_chinensis RchiOBHm_Chr4g0420511
rosa_laevigata RLG00000007755
rosa_multiflora Rmu_sc0002248.1_g000050
rosa_roxburghii Rroxscaffold_5G00363130
rosa_rugosa Rorug04G0164700 Rorug04G0164700
rosa_samantha Rh4BG228800
rosa_wichuraiana Rw4G019520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 44
AccII CGCG 1 cut(s) 11
AciI CCGC 4 cut(s) 11, 47, 59, 62
AclWI GGATC 2 cut(s) 23, 28
AcsI RAATTY 2 cut(s) 263, 293
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 1 cut(s) 233
AfiI CCNNNNNNNGG 1 cut(s) 43
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 1 cut(s) 152
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 2 cut(s) 23, 28
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 1 cut(s) 102
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 2 cut(s) 20, 174
ApoI RAATTY 2 cut(s) 263, 293
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 127
AsuHPI GGTGA 1 cut(s) 175
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 2 cut(s) 32, 161
BccI CCATC 1 cut(s) 217
BceAI ACGGC 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 152
BglII AGATCT 1 cut(s) 196
BisI GCNGC 4 cut(s) 21, 60, 63, 175
BlsI GCNGC 4 cut(s) 22, 61, 64, 176
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 1 cut(s) 129
BmsI GCATC 2 cut(s) 74, 230
BoxI GACNNNNGTC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 154
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 201
BseDI CCNNGG 1 cut(s) 154
BseGI GGATG 2 cut(s) 232, 245
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 43
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 215
BseXI GCAGC 2 cut(s) 32, 161
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 215
BsiHKCI CYCGRG 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 152
BsnI GGCC 1 cut(s) 104
BsoBI CYCGRG 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 3 cut(s) 28, 33, 196
BspACI CCGC 4 cut(s) 11, 47, 59, 62
BspANI GGCC 1 cut(s) 104
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 129
BspPI GGATC 2 cut(s) 23, 28
BssECI CCNNGG 1 cut(s) 154
BssMI GATC 3 cut(s) 28, 33, 196
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 154
BstACI GRCGYC 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 7
BstF5I GGATG 2 cut(s) 232, 245
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 3 cut(s) 31, 36, 199
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 3 cut(s) 28, 33, 196
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 247
BstPAI GACNNNNGTC 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 32, 161
BstX2I RGATCY 2 cut(s) 28, 196
BstYI RGATCY 2 cut(s) 28, 196
BsuRI GGCC 1 cut(s) 104
BtsCI GGATG 2 cut(s) 232, 245
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 2 cut(s) 26, 166
Csp6I GTAC 1 cut(s) 232
CviAII CATG 1 cut(s) 244
CviJI RGCY 1 cut(s) 104
CviKI_1 RGCY 1 cut(s) 104
CviQI GTAC 1 cut(s) 232
DpnI GATC 3 cut(s) 30, 35, 198
DpnII GATC 3 cut(s) 28, 33, 196
Eco130I CCWWGG 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 293
EcoT14I CCWWGG 1 cut(s) 154
EcoT22I ATGCAT 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 1 cut(s) 247
FaiI YATR 2 cut(s) 245, 274
FatI CATG 1 cut(s) 243
Fnu4HI GCNGC 4 cut(s) 21, 60, 63, 175
FokI GGATG 2 cut(s) 239, 252
Fsp4HI GCNGC 4 cut(s) 21, 60, 63, 175
GlaI GCGC 1 cut(s) 10
GluI GCNGC 4 cut(s) 21, 60, 63, 175
HaeIII GGCC 1 cut(s) 104
HgaI GACGC 2 cut(s) 26, 166
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 2 cut(s) 16, 213
Hpy188I TCNGA 3 cut(s) 33, 83, 207
Hpy188III TCNNGA 4 cut(s) 37, 137, 149, 298
Hpy8I GTNNAC 2 cut(s) 16, 213
Hpy99I CGWCG 2 cut(s) 43, 46
HpyAV CCTTC 2 cut(s) 115, 118
HpyCH4IV ACGT 3 cut(s) 41, 99, 234
HpyCH4V TGCA 3 cut(s) 117, 213, 243
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpySE526I ACGT 3 cut(s) 41, 99, 234
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 1 cut(s) 9
Kzo9I GATC 3 cut(s) 28, 33, 196
LpnPI CCDG 2 cut(s) 9, 272
Lsp1109I GCAGC 2 cut(s) 32, 161
LweI GCATC 2 cut(s) 74, 230
MaeII ACGT 3 cut(s) 41, 99, 234
MaeIII GTNAC 1 cut(s) 166
MalI GATC 3 cut(s) 30, 35, 198
MboI GATC 3 cut(s) 28, 33, 196
MboII GAAGA 2 cut(s) 133, 191
MflI RGATCY 2 cut(s) 28, 196
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 2 cut(s) 263, 293
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 5 cut(s) 85, 119, 191, 201, 283
Mph1103I ATGCAT 1 cut(s) 245
MslI CAYNNNNRTG 1 cut(s) 316
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 17
NdeII GATC 3 cut(s) 28, 33, 196
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 245
NspI RCATGY 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PkrI GCNGC 4 cut(s) 22, 61, 64, 176
PpuMI RGGWCCY 1 cut(s) 127
PshAI GACNNNNGTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 127
PspN4I GGNNCC 1 cut(s) 129
PspPI GGNCC 1 cut(s) 127
PspPPI RGGWCCY 1 cut(s) 127
PsuI RGATCY 2 cut(s) 28, 196
RsaI GTAC 1 cut(s) 233
RsaNI GTAC 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 316
SatI GCNGC 4 cut(s) 21, 60, 63, 175
Sau3AI GATC 3 cut(s) 28, 33, 196
Sau96I GGNCC 1 cut(s) 127
SduI GDGCHC 1 cut(s) 215
SetI ASST 6 cut(s) 44, 102, 160, 188, 212, 237
SfaNI GCATC 2 cut(s) 74, 230
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 2 cut(s) 263, 293
SsiI CCGC 4 cut(s) 11, 47, 59, 62
StyI CCWWGG 1 cut(s) 154
TaiI ACGT 3 cut(s) 44, 102, 237
TaqI TCGA 2 cut(s) 150, 297
TasI AATT 2 cut(s) 263, 293
TauI GCSGC 2 cut(s) 62, 65
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 2 cut(s) 20, 174
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 2 cut(s) 191, 276
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 2 cut(s) 263, 293
XceI RCATGY 1 cut(s) 247
ZraI GACGTC 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.