MD16G1177400.v1.1

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013137
Physical Location & Seq
Forward (+)
15082146 .. 15084450
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1177400.v1.1.491

Sequence Viewer

Length: 321 bp
ATGGCTAGCGCGGTGGGCCCAGCGGGAGAACCAATCCCAGCGTCGGCGGTGCTGACGGCGGCGGCGAAGCACATTCAGTTCAACTGCCAGGCCGAGAACGTGGCGTTTCTCAAGTGCAAGAAGAAGGACCCGAACCCGGAGAAGTGTCTCGACAAAGGTCGTCAGGTCACCCGATGCGTGCTCACCCTGCTGAAGGATCTTCACCAGAGGTGCACAACGGAGATGGATGGTTACGTTGGATGCATATATTACAATACAAATGAGTTTGATTTATGTCGCAAAGAGCAGGAGGAATTCGAGAAGAAGTGCCCGTTGGCATGA

Protein Analysis

107

Amino Acids

11.82

Weight (kDa)

7.52

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHCH PF06747 71 - 103 1.3e-07 CHCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18800 AT5G18800
fragaria_vesca FvH4_4g16930
malus_domestica MD05G1041600.v1.1 MD10G1048000.v1.1 MD13G1175600.v1.1 MD16G1177400.v1.1
prunus_persica Prupe.1G144300_v2.0.a1
pyrus_communis pycom05g03430 pycom10g03400
rosa_chinensis RchiOBHm_Chr4g0420511
rosa_laevigata RLG00000007755
rosa_multiflora Rmu_sc0002248.1_g000050
rosa_roxburghii Rroxscaffold_5G00363130
rosa_rugosa Rorug04G0164700 Rorug04G0164700
rosa_samantha Rh4BG228800
rosa_wichuraiana Rw4G019520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 11
AciI CCGC 5 cut(s) 11, 23, 47, 59, 62
AclWI GGATC 1 cut(s) 204
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 1 cut(s) 212
AfiI CCNNNNNNNGG 3 cut(s) 43, 136, 193
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 1 cut(s) 87
AjuI GAANNNNNNNTTGG 1 cut(s) 296
Alw21I GWGCWC 2 cut(s) 183, 215
Alw26I GTCTC 1 cut(s) 152
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 1 cut(s) 204
AoxI GGCC 2 cut(s) 16, 90
ApaI GGGCCC 1 cut(s) 20
ApaLI GTGCAC 1 cut(s) 211
ApoI RAATTY 1 cut(s) 293
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 3 cut(s) 16, 17, 127
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 3 cut(s) 160, 175, 194
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 1 cut(s) 127
BaeGI GKGCMC 3 cut(s) 20, 215, 311
BanII GRGCYC 1 cut(s) 20
Bbv12I GWGCWC 2 cut(s) 183, 215
BccI CCATC 2 cut(s) 217, 221
BceAI ACGGC 1 cut(s) 72
BciT130I CCWGG 1 cut(s) 89
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 1 cut(s) 152
BfaI CTAG 1 cut(s) 6
BisI GCNGC 2 cut(s) 60, 63
BlsI GCNGC 2 cut(s) 61, 64
Bme1390I CCNGG 2 cut(s) 89, 137
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 3 cut(s) 16, 17, 127
BmiI GGNNCC 2 cut(s) 18, 129
BmrFI CCNGG 2 cut(s) 89, 137
BmsI GCATC 2 cut(s) 164, 230
BmtI GCTAGC 1 cut(s) 9
BoxI GACNNNNGTC 1 cut(s) 156
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 137
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 136, 193
BseBI CCWGG 1 cut(s) 89
BseGI GGATG 2 cut(s) 232, 245
BseLI CCNNNNNNNGG 3 cut(s) 43, 136, 193
BseSI GKGCMC 3 cut(s) 20, 215, 311
BseYI CCCAGC 2 cut(s) 19, 37
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 2 cut(s) 18, 92
BsiHKAI GWGCWC 2 cut(s) 183, 215
BsiSI CCGG 1 cut(s) 137
BslI CCNNNNNNNGG 3 cut(s) 43, 136, 193
BsmAI GTCTC 1 cut(s) 152
BsnI GGCC 2 cut(s) 18, 92
Bsp120I GGGCCC 1 cut(s) 16
Bsp1286I GDGCHC 4 cut(s) 20, 183, 215, 311
Bsp143I GATC 1 cut(s) 196
BspACI CCGC 5 cut(s) 11, 23, 47, 59, 62
BspANI GGCC 2 cut(s) 18, 92
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 2 cut(s) 18, 129
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 204
BssMI GATC 1 cut(s) 196
Bst2UI CCWGG 1 cut(s) 89
BstC8I GCNNGC 2 cut(s) 7, 179
BstEII GGTNACC 1 cut(s) 166
BstENI CCTNNNNNAGG 1 cut(s) 191
BstF5I GGATG 2 cut(s) 232, 245
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 1 cut(s) 199
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 1 cut(s) 196
BstMWI GCNNNNNNNGC 2 cut(s) 15, 187
BstNI CCWGG 1 cut(s) 89
BstPAI GACNNNNGTC 1 cut(s) 156
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 2 cut(s) 87, 135
BstSLI GKGCMC 3 cut(s) 20, 215, 311
BstUI CGCG 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 2 cut(s) 18, 92
BtsCI GGATG 2 cut(s) 232, 245
Cac8I GCNNGC 2 cut(s) 7, 179
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 3 cut(s) 16, 17, 127
CseI GACGC 1 cut(s) 30
CviAII CATG 1 cut(s) 318
CviJI RGCY 3 cut(s) 5, 18, 92
CviKI_1 RGCY 3 cut(s) 5, 18, 92
DpnI GATC 1 cut(s) 198
DpnII GATC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 212
Eco91I GGTNACC 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 87
EcoT22I ATGCAT 1 cut(s) 245
EcoT38I GRGCYC 1 cut(s) 20
FaeI CATG 1 cut(s) 321
FaiI YATR 4 cut(s) 245, 247, 274, 319
FatI CATG 1 cut(s) 317
FauI CCCGC 1 cut(s) 16
Fnu4HI GCNGC 2 cut(s) 60, 63
FokI GGATG 2 cut(s) 239, 252
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 2 cut(s) 60, 63
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 10
GluI GCNGC 2 cut(s) 60, 63
GsaI CCCAGC 2 cut(s) 23, 41
HaeIII GGCC 2 cut(s) 18, 92
HapII CCGG 1 cut(s) 137
HgaI GACGC 1 cut(s) 30
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 321
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HpaII CCGG 1 cut(s) 137
HphI GGTGA 3 cut(s) 160, 175, 194
Hpy166II GTNNAC 1 cut(s) 213
Hpy188III TCNNGA 2 cut(s) 149, 298
Hpy8I GTNNAC 1 cut(s) 213
Hpy99I CGWCG 1 cut(s) 46
HpyAV CCTTC 2 cut(s) 118, 187
HpyCH4IV ACGT 2 cut(s) 99, 234
HpyCH4V TGCA 3 cut(s) 117, 213, 243
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 187
HpySE526I ACGT 2 cut(s) 99, 234
Hsp92II CATG 1 cut(s) 321
HspAI GCGC 1 cut(s) 9
Kzo9I GATC 1 cut(s) 196
LpnPI CCDG 9 cut(s) 33, 51, 74, 101, 149, 150, 200, 218, 272
LweI GCATC 2 cut(s) 164, 230
MaeI CTAG 1 cut(s) 6
MaeII ACGT 2 cut(s) 99, 234
MaeIII GTNAC 2 cut(s) 166, 230
MalI GATC 1 cut(s) 198
MboI GATC 1 cut(s) 196
MboII GAAGA 3 cut(s) 133, 191, 313
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 4 cut(s) 20, 183, 215, 311
MluCI AATT 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 2 cut(s) 201, 283
Mph1103I ATGCAT 1 cut(s) 245
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 89, 137
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 2 cut(s) 15, 187
NciI CCSGG 1 cut(s) 137
NdeII GATC 1 cut(s) 196
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 321
NlaIV GGNNCC 2 cut(s) 18, 129
NmeAIII GCCGAG 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 245
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PkrI GCNGC 2 cut(s) 61, 64
PpuMI RGGWCCY 1 cut(s) 127
PshAI GACNNNNGTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 87
PspEI GGTNACC 1 cut(s) 166
PspFI CCCAGC 2 cut(s) 19, 37
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 18, 129
PspOMI GGGCCC 1 cut(s) 16
PspPI GGNCC 3 cut(s) 16, 17, 127
PspPPI RGGWCCY 1 cut(s) 127
PsuI RGATCY 1 cut(s) 196
SatI GCNGC 2 cut(s) 60, 63
Sau3AI GATC 1 cut(s) 196
Sau96I GGNCC 3 cut(s) 16, 17, 127
ScrFI CCNGG 2 cut(s) 89, 137
SduI GDGCHC 4 cut(s) 20, 183, 215, 311
SetI ASST 5 cut(s) 102, 160, 168, 212, 237
SfaNI GCATC 2 cut(s) 164, 230
SinI GGWCC 1 cut(s) 127
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 1 cut(s) 293
SsiI CCGC 5 cut(s) 11, 23, 47, 59, 62
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 2 cut(s) 87, 135
TaiI ACGT 2 cut(s) 102, 237
TaqI TCGA 2 cut(s) 150, 297
TasI AATT 1 cut(s) 293
TauI GCSGC 2 cut(s) 62, 65
TseFI GTSAC 1 cut(s) 166
Tsp45I GTSAC 1 cut(s) 166
TspGWI ACGGA 1 cut(s) 233
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 127
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 1 cut(s) 293
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.