MD13G1175600.v1.1

NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013137
Physical Location & Seq
Forward (+)
14515298 .. 14517201
1904 bp
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UTR
Exon/CDS
Intron
MD13G1175600.v1.1.491

Sequence Viewer

Length: 321 bp
ATGGCGAGCGCGGTGGACCCAGCGGGAGATCCAATCCCAACGTCGGCGGTGCTGACGGCGGCGGCGAAGCACATTCAGTTCAGCTGCCAGGCCGAGAACGTGGCTTTCCTCAAGTGCAAGAAGAAGGACCCGAACCCCGACAAGTGTCTCGACAAAGGTCGTCAGGTCACCCGATGCGTCCTCACCCTGCTGAAAGATCTTCATCAGAGGTGCACAAAGGAGATGGATGCTTACGTCGGGTGCATGTATTACAATACAAATGAGTTTGATTTATGTCGCACAGAGCAGGAGGAATTCGAGAAAAAGTGCCCATTGGCATGA

Protein Analysis

107

Amino Acids

11.89

Weight (kDa)

6.7

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHCH PF06747 71 - 103 8e-07 CHCH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18800 AT5G18800
fragaria_vesca FvH4_4g16930
malus_domestica MD05G1041600.v1.1 MD10G1048000.v1.1 MD13G1175600.v1.1 MD16G1177400.v1.1
prunus_persica Prupe.1G144300_v2.0.a1
pyrus_communis pycom05g03430 pycom10g03400
rosa_chinensis RchiOBHm_Chr4g0420511
rosa_laevigata RLG00000007755
rosa_multiflora Rmu_sc0002248.1_g000050
rosa_roxburghii Rroxscaffold_5G00363130
rosa_rugosa Rorug04G0164700 Rorug04G0164700
rosa_samantha Rh4BG228800
rosa_wichuraiana Rw4G019520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 11
AciI CCGC 5 cut(s) 11, 23, 47, 59, 62
AclWI GGATC 1 cut(s) 23
AcsI RAATTY 1 cut(s) 293
AfiI CCNNNNNNNGG 1 cut(s) 43
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 1 cut(s) 84
AluI AGCT 1 cut(s) 84
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 1 cut(s) 152
Alw44I GTGCAC 1 cut(s) 211
AlwI GGATC 1 cut(s) 23
AoxI GGCC 1 cut(s) 90
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 1 cut(s) 84
ApoI RAATTY 1 cut(s) 293
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 16, 127
AsuHPI GGTGA 2 cut(s) 160, 175
AvaII GGWCC 2 cut(s) 16, 127
BaeGI GKGCMC 2 cut(s) 215, 311
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 71
BccI CCATC 1 cut(s) 217
BceAI ACGGC 1 cut(s) 72
BciT130I CCWGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 152
BglII AGATCT 1 cut(s) 196
BisI GCNGC 3 cut(s) 60, 63, 85
BlsI GCNGC 3 cut(s) 61, 64, 86
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 2 cut(s) 16, 127
BmgT120I GGNCC 2 cut(s) 16, 127
BmiI GGNNCC 2 cut(s) 18, 129
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 2 cut(s) 164, 217
BoxI GACNNNNGTC 2 cut(s) 144, 156
BpuEI CTTGAG 1 cut(s) 95
BsaBI GATNNNNATC 1 cut(s) 201
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 1 cut(s) 89
BseGI GGATG 1 cut(s) 232
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 1 cut(s) 43
BseSI GKGCMC 2 cut(s) 215, 311
BseXI GCAGC 1 cut(s) 71
BseYI CCCAGC 1 cut(s) 19
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 152
BsnI GGCC 1 cut(s) 92
Bsp1286I GDGCHC 2 cut(s) 215, 311
Bsp143I GATC 2 cut(s) 28, 196
BspACI CCGC 5 cut(s) 11, 23, 47, 59, 62
BspANI GGCC 1 cut(s) 92
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 2 cut(s) 18, 129
BspPI GGATC 1 cut(s) 23
BssMI GATC 2 cut(s) 28, 196
Bst2UI CCWGG 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 7
BstEII GGTNACC 1 cut(s) 166
BstF5I GGATG 1 cut(s) 232
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 2 cut(s) 31, 199
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 2 cut(s) 28, 196
BstNI CCWGG 1 cut(s) 89
BstNSI RCATGY 1 cut(s) 247
BstPAI GACNNNNGTC 2 cut(s) 144, 156
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 87
BstSLI GKGCMC 2 cut(s) 215, 311
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 1 cut(s) 71
BstX2I RGATCY 2 cut(s) 28, 196
BstYI RGATCY 2 cut(s) 28, 196
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 16, 127
CseI GACGC 1 cut(s) 166
CviAII CATG 2 cut(s) 244, 318
CviJI RGCY 3 cut(s) 84, 92, 104
CviKI_1 RGCY 3 cut(s) 84, 92, 104
DpnI GATC 2 cut(s) 30, 198
DpnII GATC 2 cut(s) 28, 196
Eco47I GGWCC 2 cut(s) 16, 127
Eco91I GGTNACC 1 cut(s) 166
EcoO109I RGGNCCY 1 cut(s) 127
EcoO65I GGTNACC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 87
FaeI CATG 2 cut(s) 247, 321
FaiI YATR 3 cut(s) 245, 274, 319
FatI CATG 2 cut(s) 243, 317
FauI CCCGC 1 cut(s) 16
Fnu4HI GCNGC 3 cut(s) 60, 63, 85
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 3 cut(s) 60, 63, 85
GlaI GCGC 1 cut(s) 10
GluI GCNGC 3 cut(s) 60, 63, 85
GsaI CCCAGC 1 cut(s) 23
HaeIII GGCC 1 cut(s) 92
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 11
Hin1II CATG 2 cut(s) 247, 321
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HphI GGTGA 2 cut(s) 160, 175
Hpy166II GTNNAC 2 cut(s) 16, 213
Hpy188I TCNGA 1 cut(s) 207
Hpy188III TCNNGA 2 cut(s) 149, 298
Hpy8I GTNNAC 2 cut(s) 16, 213
Hpy99I CGWCG 2 cut(s) 46, 239
HpyAV CCTTC 1 cut(s) 118
HpyCH4IV ACGT 3 cut(s) 41, 99, 234
HpyCH4V TGCA 3 cut(s) 117, 213, 243
HpySE526I ACGT 3 cut(s) 41, 99, 234
Hsp92II CATG 2 cut(s) 247, 321
HspAI GCGC 1 cut(s) 9
Kzo9I GATC 2 cut(s) 28, 196
LpnPI CCDG 6 cut(s) 33, 74, 101, 149, 200, 272
Lsp1109I GCAGC 1 cut(s) 71
LweI GCATC 2 cut(s) 164, 217
MaeII ACGT 3 cut(s) 41, 99, 234
MaeIII GTNAC 1 cut(s) 166
MalI GATC 2 cut(s) 30, 198
MboI GATC 2 cut(s) 28, 196
MboII GAAGA 2 cut(s) 133, 191
MflI RGATCY 2 cut(s) 28, 196
MhlI GDGCHC 2 cut(s) 215, 311
MluCI AATT 1 cut(s) 293
MnlI CCTC 4 cut(s) 119, 191, 201, 283
MslI CAYNNNNRTG 1 cut(s) 316
MspA1I CMGCKG 2 cut(s) 23, 84
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 11
NdeII GATC 2 cut(s) 28, 196
NlaIII CATG 2 cut(s) 247, 321
NlaIV GGNNCC 2 cut(s) 18, 129
NmeAIII GCCGAG 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 62
PkrI GCNGC 3 cut(s) 61, 64, 86
PpuMI RGGWCCY 1 cut(s) 127
PshAI GACNNNNGTC 2 cut(s) 144, 156
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 87
PspEI GGTNACC 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 2 cut(s) 18, 129
PspPI GGNCC 2 cut(s) 16, 127
PspPPI RGGWCCY 1 cut(s) 127
PsuI RGATCY 2 cut(s) 28, 196
PvuII CAGCTG 1 cut(s) 84
RseI CAYNNNNRTG 1 cut(s) 316
SatI GCNGC 3 cut(s) 60, 63, 85
Sau3AI GATC 2 cut(s) 28, 196
Sau96I GGNCC 2 cut(s) 16, 127
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 2 cut(s) 215, 311
SetI ASST 7 cut(s) 44, 86, 102, 160, 168, 212, 237
SfaNI GCATC 2 cut(s) 164, 217
SinI GGWCC 2 cut(s) 16, 127
SmiMI CAYNNNNRTG 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 1 cut(s) 293
SsiI CCGC 5 cut(s) 11, 23, 47, 59, 62
StyD4I CCNGG 1 cut(s) 87
TaiI ACGT 3 cut(s) 44, 102, 237
TaqI TCGA 2 cut(s) 150, 297
TasI AATT 1 cut(s) 293
TauI GCSGC 2 cut(s) 62, 65
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 1 cut(s) 84
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 1 cut(s) 191
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 16, 127
XapI RAATTY 1 cut(s) 293
XceI RCATGY 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.