FvH4_4g17260

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017286
Physical Location & Seq
Reverse (-)
21232402 .. 21233804
1403 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g17260.t1

Sequence Viewer

Length: 636 bp
ATGGGAAGAACAAAGAAGCCTGCAGTTTACTTTTGCTTGAGTTTTATTTTCCGGATGTTGTTGATTGCTGCCTCGACAATTAGCGCTGTGACCATGGCGACTAGCAATGATACCGAAGATTGGTACGATCTAGGGACTGGCTGGACTGGCTATTCCAGCTATGCATCCGATTCTGTCAAATTCACTGATTCACCCTCATTCATATATTTTGTTGCAGCATTGTCTGTGGCGGGGTTTTACGGCATCATTACCAAGTTATCTACTTGTTTACTCATCTGCTTAGCGCCATCTTCATCAATAATGTTGGTTATCTTTGCGTTTTTTGATGTGTTGATATTGGGGACAGTAGCCTCAGCAACTGGGACTGCCGGTAGTGTTGCATACAGCATATTCGTGGGTGATCTTTGCTCTACATACAGCCGCTTCTGTCGACATCTAGGAGTTGCCTTGGGGTTCGCCTTATTCGCCTCACTTTTGCTTGTCATTCTTCTATGGACCTCCCTCATTAGTCTCTACAAAAGGATCCCCAAACAAACCTTTGAGACATCACAACCTCTTGCACAGTCTCCGTTACAAACTCCACCACAAGCGCAGACGACCTTAATTACAGACTCCACCACAAGCACAAGGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

212

Amino Acids

22.8

Weight (kDa)

8.27

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 13 - 159 6.3e-19 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017286)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g17260
malus_domestica MD13G1173400.v1.1
prunus_persica Prupe.1G141600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0421181
rosa_laevigata RLG00000007708
rosa_multiflora Rmu_sc0009290.1_g000009
rosa_roxburghii Rroxscaffold_5G00363480
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229800 Rh4BG232500 Rh4CG244100 Rh4DG228300
rosa_wichuraiana Rw4G020400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 430
AccIII TCCGGA 1 cut(s) 51
AciI CCGC 2 cut(s) 230, 421
AclWI GGATC 2 cut(s) 517, 530
AcsI RAATTY 1 cut(s) 179
AfaI GTAC 1 cut(s) 125
AfeI AGCGCT 1 cut(s) 85
AfiI CCNNNNNNNGG 1 cut(s) 120
AluBI AGCT 1 cut(s) 159
AluI AGCT 1 cut(s) 159
Alw26I GTCTC 3 cut(s) 515, 536, 570
AlwI GGATC 2 cut(s) 517, 530
AlwNI CAGNNNCTG 1 cut(s) 359
Aor13HI TCCGGA 1 cut(s) 51
Aor51HI AGCGCT 1 cut(s) 85
ApeKI GCWGC 2 cut(s) 68, 215
ApoI RAATTY 1 cut(s) 179
AspLEI GCGC 3 cut(s) 86, 286, 592
AspS9I GGNCC 1 cut(s) 495
AsuHPI GGTGA 2 cut(s) 183, 410
AvaII GGWCC 1 cut(s) 495
BamHI GGATCC 1 cut(s) 522
BbvCI CCTCAGC 1 cut(s) 352
BbvI GCAGC 2 cut(s) 55, 227
BccI CCATC 1 cut(s) 295
BceAI ACGGC 1 cut(s) 256
BcoDI GTCTC 3 cut(s) 515, 536, 570
BfaI CTAG 3 cut(s) 102, 131, 437
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 2 cut(s) 87, 287
BisI GCNGC 3 cut(s) 69, 216, 421
BlpI GCTNAGC 1 cut(s) 280
BlsI GCNGC 3 cut(s) 70, 217, 422
Bme18I GGWCC 1 cut(s) 495
BmgT120I GGNCC 1 cut(s) 495
BmiI GGNNCC 1 cut(s) 524
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 2 cut(s) 173, 252
BmuI ACTGGG 1 cut(s) 369
Bpu10I CCTNAGC 1 cut(s) 352
Bpu1102I GCTNAGC 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 58
BsaJI CCNNGG 2 cut(s) 93, 447
BsaWI WCCGGW 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse118I RCCGGY 1 cut(s) 368
Bse1I ACTGG 3 cut(s) 142, 151, 364
Bse3DI GCAATG 1 cut(s) 112
BseAI TCCGGA 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 93, 447
BseGI GGATG 2 cut(s) 60, 164
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMI GCAATG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 3 cut(s) 142, 151, 364
BseXI GCAGC 2 cut(s) 55, 227
BsiSI CCGG 2 cut(s) 52, 369
BslFI GGGAC 3 cut(s) 148, 355, 376
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 3 cut(s) 515, 536, 570
BsmFI GGGAC 3 cut(s) 148, 355, 376
Bsp13I TCCGGA 1 cut(s) 51
Bsp143I GATC 3 cut(s) 127, 400, 522
Bsp1720I GCTNAGC 1 cut(s) 280
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 2 cut(s) 230, 421
BspCNI CTCAG 1 cut(s) 365
BspEI TCCGGA 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 524
BspMAI CTGCAG 1 cut(s) 25
BspPI GGATC 2 cut(s) 517, 530
BsrDI GCAATG 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 368
BsrI ACTGG 3 cut(s) 142, 151, 364
BssAI RCCGGY 1 cut(s) 368
BssECI CCNNGG 2 cut(s) 93, 447
BssMI GATC 3 cut(s) 127, 400, 522
BssT1I CCWWGG 2 cut(s) 93, 447
Bst4CI ACNGT 2 cut(s) 346, 564
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 2 cut(s) 280, 352
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 2 cut(s) 60, 164
BstH2I RGCGCY 2 cut(s) 87, 287
BstHHI GCGC 3 cut(s) 86, 286, 592
BstKTI GATC 3 cut(s) 130, 403, 525
BstMAI GTCTC 3 cut(s) 515, 536, 570
BstMBI GATC 3 cut(s) 127, 400, 522
BstMWI GCNNNNNNNGC 3 cut(s) 147, 156, 464
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 55, 227
BstX2I RGATCY 1 cut(s) 522
BstYI RGATCY 1 cut(s) 522
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 2 cut(s) 60, 164
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 21
CaiI CAGNNNCTG 1 cut(s) 359
CfoI GCGC 3 cut(s) 86, 286, 592
Cfr10I RCCGGY 1 cut(s) 368
Cfr13I GGNCC 1 cut(s) 495
Csp6I GTAC 1 cut(s) 124
CviAII CATG 1 cut(s) 94
CviJI RGCY 6 cut(s) 19, 141, 150, 159, 350, 420
CviKI_1 RGCY 6 cut(s) 19, 141, 150, 159, 350, 420
CviQI GTAC 1 cut(s) 124
DdeI CTNAG 2 cut(s) 280, 352
DpnI GATC 3 cut(s) 129, 402, 524
DpnII GATC 3 cut(s) 127, 400, 522
Eco130I CCWWGG 2 cut(s) 93, 447
Eco47I GGWCC 1 cut(s) 495
Eco47III AGCGCT 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 93, 447
EcoT22I ATGCAT 1 cut(s) 166
ErhI CCWWGG 2 cut(s) 93, 447
FaeI CATG 1 cut(s) 97
FaiI YATR 8 cut(s) 95, 162, 203, 205, 382, 389, 415, 493
FaqI GGGAC 3 cut(s) 148, 355, 376
FatI CATG 1 cut(s) 93
FauI CCCGC 1 cut(s) 223
FblI GTMKAC 1 cut(s) 430
Fnu4HI GCNGC 3 cut(s) 69, 216, 421
FokI GGATG 2 cut(s) 67, 151
Fsp4HI GCNGC 3 cut(s) 69, 216, 421
FspBI CTAG 3 cut(s) 102, 131, 437
GlaI GCGC 3 cut(s) 85, 285, 591
GluI GCNGC 3 cut(s) 69, 216, 421
HaeII RGCGCY 2 cut(s) 87, 287
HapII CCGG 2 cut(s) 52, 369
HhaI GCGC 3 cut(s) 86, 286, 592
Hin1II CATG 1 cut(s) 97
Hin6I GCGC 3 cut(s) 84, 284, 590
HinP1I GCGC 3 cut(s) 84, 284, 590
HincII GTYRAC 1 cut(s) 431
HindII GTYRAC 1 cut(s) 431
HinfI GANTC 3 cut(s) 170, 188, 611
HpaII CCGG 2 cut(s) 52, 369
HphI GGTGA 2 cut(s) 183, 410
Hpy166II GTNNAC 3 cut(s) 28, 269, 431
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 1 cut(s) 52
Hpy8I GTNNAC 3 cut(s) 28, 269, 431
HpyCH4III ACNGT 2 cut(s) 346, 564
HpyCH4V TGCA 5 cut(s) 23, 164, 215, 380, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 147, 156, 464
HpyF3I CTNAG 2 cut(s) 280, 352
Hsp92II CATG 1 cut(s) 97
HspAI GCGC 3 cut(s) 84, 284, 590
Kpn2I TCCGGA 1 cut(s) 51
Kzo9I GATC 3 cut(s) 127, 400, 522
LpnPI CCDG 8 cut(s) 33, 65, 123, 127, 132, 169, 345, 382
Lsp1109I GCAGC 2 cut(s) 55, 227
LweI GCATC 2 cut(s) 173, 252
MaeI CTAG 3 cut(s) 102, 131, 437
MaeIII GTNAC 2 cut(s) 88, 570
MalI GATC 3 cut(s) 129, 402, 524
MboI GATC 3 cut(s) 127, 400, 522
MboII GAAGA 4 cut(s) 18, 128, 282, 479
MflI RGATCY 1 cut(s) 522
MluCI AATT 3 cut(s) 78, 179, 603
MlyI GAGTC 1 cut(s) 605
MnlI CCTC 7 cut(s) 82, 205, 361, 478, 508, 512, 564
Mph1103I ATGCAT 1 cut(s) 166
MroI TCCGGA 1 cut(s) 51
MseI TTAA 1 cut(s) 602
MslI CAYNNNNRTG 1 cut(s) 392
MspI CCGG 2 cut(s) 52, 369
MwoI GCNNNNNNNGC 3 cut(s) 147, 156, 464
NcoI CCATGG 1 cut(s) 93
NdeII GATC 3 cut(s) 127, 400, 522
NlaIII CATG 1 cut(s) 97
NlaIV GGNNCC 1 cut(s) 524
NmuCI GTSAC 1 cut(s) 88
NsiI ATGCAT 1 cut(s) 166
PfeI GAWTC 2 cut(s) 170, 188
PkrI GCNGC 3 cut(s) 70, 217, 422
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
PspN4I GGNNCC 1 cut(s) 524
PspPI GGNCC 1 cut(s) 495
PstI CTGCAG 1 cut(s) 25
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 1 cut(s) 522
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
RseI CAYNNNNRTG 1 cut(s) 392
SalI GTCGAC 1 cut(s) 429
SaqAI TTAA 1 cut(s) 602
SatI GCNGC 3 cut(s) 69, 216, 421
Sau3AI GATC 3 cut(s) 127, 400, 522
Sau96I GGNCC 1 cut(s) 495
SchI GAGTC 1 cut(s) 605
SetI ASST 5 cut(s) 161, 500, 539, 556, 602
SfaNI GCATC 2 cut(s) 173, 252
SfcI CTRYAG 1 cut(s) 21
SinI GGWCC 1 cut(s) 495
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 3 cut(s) 78, 179, 603
SsiI CCGC 2 cut(s) 230, 421
SspMI CTAG 3 cut(s) 102, 131, 437
StyI CCWWGG 2 cut(s) 93, 447
TaaI ACNGT 2 cut(s) 346, 564
TaqI TCGA 2 cut(s) 74, 430
TasI AATT 3 cut(s) 78, 179, 603
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 2 cut(s) 170, 188
Tru1I TTAA 1 cut(s) 602
Tru9I TTAA 1 cut(s) 602
TscAI CASTG 1 cut(s) 190
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 2 cut(s) 68, 215
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 2 cut(s) 190, 282
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 495
XapI RAATTY 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 430
XspI CTAG 3 cut(s) 102, 131, 437
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.