Rh4DG228300

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0017286
Physical Location & Seq
Reverse (-)
43358416 .. 43360370
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG228300.1

Sequence Viewer

Length: 750 bp
ATGTCAACTAAACACTCCTTTGAGCCTTATGGTTTTGCTCCCTATATAACAGACTGCTTAGTTAACAACAACTTGACCTTTTCACAAACACTCACAGCAGCATCATCAGCAGTCATGGGAACAACGAAGAGGCCTGTGGTTTACTTTTGCTTGAGTTTTCTTCTCCGGATGTTGTTGATCGTGGCCTCGACAATTAGTGCTGTGACCATGGTAACTAGCAAGGATACCCAATATTGGTACGATCCAGGGACTGAGTATGACAGTGGAACCTATTCCGTCAAATTCACTGATTCACCCTCATTCATATATTTTGTTGCGGCATTATCTGTGGCGGGCATTTATGGTATCATTACCAAGTTATCATCTTGTTTAGTCATCTGCTTAGCGCCATCTTCATCAATGATGTTGGTTCTCTTTGCGTTTTTTGATGTGTTGATATTGGGAGTGGTAGCCTCGGCAACTGGGAGTGCTGGTAGTGTTGCATATAGCATACTCAAGGATGATCTTTGCTCTACTTACAACCGCTACTGTCGACATCTAGGAATCGCCTTGGGGTTCGCCTTATTTGCCTCAGTTTTGCTTGTCGTTCTTCTATGGACCTCCCTAATTAGTCTCTACAAAAGGATCCCCAAACAAAACTTTGAGACACCACAACCACTTTCACAGTCTCCGTTACAAACCCCACCACAAGCGCAGACCTTACAGACTCCACCACAGGCACAAGAAGGAACTAAAACATATGCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

249

Amino Acids

27.17

Weight (kDa)

8.15

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 51 - 193 9.5e-23 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017286)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g17260
malus_domestica MD13G1173400.v1.1
prunus_persica Prupe.1G141600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0421181
rosa_laevigata RLG00000007708
rosa_multiflora Rmu_sc0009290.1_g000009
rosa_roxburghii Rroxscaffold_5G00363480
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229800 Rh4BG232500 Rh4CG244100 Rh4DG228300
rosa_wichuraiana Rw4G020400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 532
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 3 cut(s) 317, 332, 523
AclWI GGATC 3 cut(s) 236, 619, 632
AcsI RAATTY 1 cut(s) 281
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 234
AjnI CCWGG 1 cut(s) 244
Alw26I GTCTC 3 cut(s) 617, 638, 672
AlwI GGATC 3 cut(s) 236, 619, 632
AlwNI CAGNNNCTG 1 cut(s) 251
Aor13HI TCCGGA 1 cut(s) 165
AoxI GGCC 2 cut(s) 131, 183
ApeKI GCWGC 1 cut(s) 98
ApoI RAATTY 1 cut(s) 281
Asp700I GAANNNNTTC 1 cut(s) 271
AspLEI GCGC 2 cut(s) 388, 694
AspS9I GGNCC 1 cut(s) 597
AsuHPI GGTGA 1 cut(s) 285
AvaII GGWCC 1 cut(s) 597
BamHI GGATCC 1 cut(s) 624
BbvI GCAGC 1 cut(s) 110
BccI CCATC 1 cut(s) 397
BciT130I CCWGG 1 cut(s) 246
BciVI GTATCC 1 cut(s) 217
BcoDI GTCTC 3 cut(s) 617, 638, 672
BfaI CTAG 2 cut(s) 216, 539
BfoI RGCGCY 1 cut(s) 389
BfuI GTATCC 1 cut(s) 217
BisI GCNGC 2 cut(s) 99, 318
BlpI GCTNAGC 1 cut(s) 382
BlsI GCNGC 2 cut(s) 100, 319
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 1 cut(s) 597
BmgT120I GGNCC 1 cut(s) 597
BmiI GGNNCC 2 cut(s) 268, 626
BmrFI CCNGG 1 cut(s) 246
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 1 cut(s) 110
BmuI ACTGGG 1 cut(s) 471
Bpu1102I GCTNAGC 1 cut(s) 382
BpuEI CTTGAG 2 cut(s) 172, 479
BsaJI CCNNGG 4 cut(s) 207, 245, 453, 549
BsaWI WCCGGW 1 cut(s) 165
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse1I ACTGG 1 cut(s) 466
BseAI TCCGGA 1 cut(s) 165
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 4 cut(s) 207, 245, 453, 549
BseGI GGATG 2 cut(s) 174, 505
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMII CTCAG 2 cut(s) 243, 585
BseNI ACTGG 1 cut(s) 466
BseXI GCAGC 1 cut(s) 110
BshFI GGCC 2 cut(s) 133, 185
BsiSI CCGG 1 cut(s) 166
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 1 cut(s) 234
BsmAI GTCTC 3 cut(s) 617, 638, 672
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 2 cut(s) 133, 185
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 4 cut(s) 177, 241, 502, 624
Bsp1720I GCTNAGC 1 cut(s) 382
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 3 cut(s) 317, 332, 523
BspANI GGCC 2 cut(s) 133, 185
BspCNI CTCAG 2 cut(s) 244, 584
BspEI TCCGGA 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 268, 626
BspPI GGATC 3 cut(s) 236, 619, 632
BsrI ACTGG 1 cut(s) 466
BssECI CCNNGG 4 cut(s) 207, 245, 453, 549
BssMI GATC 4 cut(s) 177, 241, 502, 624
BssT1I CCWWGG 2 cut(s) 207, 549
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 3 cut(s) 263, 530, 666
Bst6I CTCTTC 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 334
BstDEI CTNAG 4 cut(s) 58, 252, 382, 571
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 174, 505
BstH2I RGCGCY 1 cut(s) 389
BstHHI GCGC 2 cut(s) 388, 694
BstKTI GATC 4 cut(s) 180, 244, 505, 627
BstMAI GTCTC 3 cut(s) 617, 638, 672
BstMBI GATC 4 cut(s) 177, 241, 502, 624
BstMWI GCNNNNNNNGC 2 cut(s) 107, 566
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 1 cut(s) 244
BstV1I GCAGC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 624
BstYI RGATCY 1 cut(s) 624
BsuI GTATCC 1 cut(s) 217
BsuRI GGCC 2 cut(s) 133, 185
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 2 cut(s) 174, 505
BtsIMutI CAGTG 2 cut(s) 268, 285
Cac8I GCNNGC 1 cut(s) 334
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 2 cut(s) 388, 694
Cfr13I GGNCC 1 cut(s) 597
Csp6I GTAC 1 cut(s) 238
CviAII CATG 2 cut(s) 115, 208
CviJI RGCY 4 cut(s) 25, 133, 185, 452
CviKI_1 RGCY 4 cut(s) 25, 133, 185, 452
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 4 cut(s) 58, 252, 382, 571
DpnI GATC 4 cut(s) 179, 243, 504, 626
DpnII GATC 4 cut(s) 177, 241, 502, 624
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco130I CCWWGG 2 cut(s) 207, 549
Eco147I AGGCCT 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 597
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 207, 549
EcoT22I ATGCAT 1 cut(s) 745
ErhI CCWWGG 2 cut(s) 207, 549
FaeI CATG 2 cut(s) 118, 211
FaqI GGGAC 1 cut(s) 262
FatI CATG 2 cut(s) 114, 207
FauI CCCGC 1 cut(s) 325
FauNDI CATATG 1 cut(s) 739
FblI GTMKAC 1 cut(s) 532
Fnu4HI GCNGC 2 cut(s) 99, 318
FokI GGATG 2 cut(s) 181, 512
Fsp4HI GCNGC 2 cut(s) 99, 318
FspBI CTAG 2 cut(s) 216, 539
GlaI GCGC 2 cut(s) 387, 693
GluI GCNGC 2 cut(s) 99, 318
HaeII RGCGCY 1 cut(s) 389
HaeIII GGCC 2 cut(s) 133, 185
HapII CCGG 1 cut(s) 166
HhaI GCGC 2 cut(s) 388, 694
Hin1II CATG 2 cut(s) 118, 211
Hin6I GCGC 2 cut(s) 386, 692
HinP1I GCGC 2 cut(s) 386, 692
HincII GTYRAC 3 cut(s) 6, 64, 533
HindII GTYRAC 3 cut(s) 6, 64, 533
HinfI GANTC 3 cut(s) 290, 543, 706
HpaI GTTAAC 1 cut(s) 64
HpaII CCGG 1 cut(s) 166
HphI GGTGA 1 cut(s) 285
Hpy166II GTNNAC 4 cut(s) 6, 64, 142, 533
Hpy188III TCNNGA 1 cut(s) 166
Hpy8I GTNNAC 4 cut(s) 6, 64, 142, 533
HpyAV CCTTC 1 cut(s) 719
HpyCH4III ACNGT 3 cut(s) 263, 530, 666
HpyCH4V TGCA 2 cut(s) 482, 743
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 566
HpyF3I CTNAG 4 cut(s) 58, 252, 382, 571
Hsp92II CATG 2 cut(s) 118, 211
HspAI GCGC 2 cut(s) 386, 692
Kpn2I TCCGGA 1 cut(s) 165
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 4 cut(s) 177, 241, 502, 624
LmnI GCTCC 1 cut(s) 43
LpnPI CCDG 7 cut(s) 147, 179, 231, 258, 447, 456, 701
Lsp1109I GCAGC 1 cut(s) 110
LweI GCATC 1 cut(s) 110
MaeI CTAG 2 cut(s) 216, 539
MaeIII GTNAC 3 cut(s) 202, 211, 672
MalI GATC 4 cut(s) 179, 243, 504, 626
MboI GATC 4 cut(s) 177, 241, 502, 624
MboII GAAGA 4 cut(s) 139, 152, 384, 581
MflI RGATCY 1 cut(s) 624
MluCI AATT 3 cut(s) 192, 281, 606
MlyI GAGTC 1 cut(s) 700
MnlI CCTC 6 cut(s) 123, 196, 307, 463, 580, 610
Mph1103I ATGCAT 1 cut(s) 745
MroI TCCGGA 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 271
MseI TTAA 2 cut(s) 63, 748
MspI CCGG 1 cut(s) 166
MspR9I CCNGG 1 cut(s) 246
MvaI CCWGG 1 cut(s) 246
MwoI GCNNNNNNNGC 2 cut(s) 107, 566
NcoI CCATGG 1 cut(s) 207
NdeI CATATG 1 cut(s) 739
NdeII GATC 4 cut(s) 177, 241, 502, 624
NlaIII CATG 2 cut(s) 118, 211
NlaIV GGNNCC 2 cut(s) 268, 626
NmeAIII GCCGAG 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 202
NsiI ATGCAT 1 cut(s) 745
PceI AGGCCT 1 cut(s) 133
PdmI GAANNNNTTC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 290, 543
PkrI GCNGC 2 cut(s) 100, 319
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 268, 626
PspPI GGNCC 1 cut(s) 597
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 1 cut(s) 624
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SalI GTCGAC 1 cut(s) 531
SaqAI TTAA 2 cut(s) 63, 748
SatI GCNGC 2 cut(s) 99, 318
Sau3AI GATC 4 cut(s) 177, 241, 502, 624
Sau96I GGNCC 1 cut(s) 597
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 1 cut(s) 246
SetI ASST 4 cut(s) 80, 272, 602, 701
SfaNI GCATC 1 cut(s) 110
SinI GGWCC 1 cut(s) 597
SmlI CTYRAG 2 cut(s) 151, 494
SmoI CTYRAG 2 cut(s) 151, 494
Sse9I AATT 3 cut(s) 192, 281, 606
SseBI AGGCCT 1 cut(s) 133
SsiI CCGC 3 cut(s) 317, 332, 523
SspI AATATT 1 cut(s) 233
SspMI CTAG 2 cut(s) 216, 539
StuI AGGCCT 1 cut(s) 133
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 2 cut(s) 207, 549
TaaI ACNGT 3 cut(s) 263, 530, 666
TaqI TCGA 2 cut(s) 188, 532
TasI AATT 3 cut(s) 192, 281, 606
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 2 cut(s) 290, 543
Tru1I TTAA 2 cut(s) 63, 748
Tru9I TTAA 2 cut(s) 63, 748
TscAI CASTG 2 cut(s) 268, 292
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 1 cut(s) 98
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 2 cut(s) 292, 384
TspGWI ACGGA 2 cut(s) 265, 660
TspRI CASTG 2 cut(s) 268, 292
VpaK11BI GGWCC 1 cut(s) 597
XapI RAATTY 1 cut(s) 281
XmiI GTMKAC 1 cut(s) 532
XmnI GAANNNNTTC 1 cut(s) 271
XspI CTAG 2 cut(s) 216, 539
Zsp2I ATGCAT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.