RchiOBHm_Chr4g0421181

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017286
Physical Location & Seq
Reverse (-)
46843223 .. 46844505
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39084

Sequence Viewer

Length: 768 bp
ATGGCCGGCCGGTGGAGAATGTCAACTAAACACTCCTTTGAGCCTTATGGTTTTGCTCCCTATATAACACAGTGCTTAGTTAACAACAACTTGACCTATTCACAAACACTCACAGCAGCATCATCAGCAGTCATGGGAACAACGAAGAGGCCTGTGGTTTACTTTTGCTTGAGTTTTCTTCTCCGGATGTTGTTGATCGTGGCCTCGACAATTAGTGCTGTGACCATGGTAACTAGCAAGGATACCCAATATTGGTACGATCCAGGGACTGAGTATGACAGTGGAACCTATTCCGTCAAATTCACTGATTCACCCTCATTCATATATTTTGTTGCGGCATTATCTGTGGCGGGCGTTTATGGTATCATTACCAAGTTATCATCTTGTTTAGTCATCTGCTTAGCGCCATCTTCATCAATGATGTTGGTTCTCTTTGCGTTTTTTGATGTGTTGATATTGGGAGTGGTAGCCTCAGCAACTGGGAGTGCCGGTAGTGTTGCATATAGCATACTCAAGGGTGATCTTTGCTCTACTTACAACCGCTACTGTCGACATCTAGGAGTCGCCTTGGGGTTCGCCTTATTTTCCTCAGTTTTGCTTGTCGTTCTTCTATGGACCTCCCTAATTAGTCTCTACAAAAGGATCCCCAAACAAAACTTTGAGACACCACAACCACTTTCACAGTCTCCGTTACAAACCCCACCACAAGCGCAGACCTTACAGACTCCACCACAGGCACAAGAAGGAACTAAAACATATGCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

255

Amino Acids

27.89

Weight (kDa)

8.97

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 57 - 199 3.6e-23 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017286)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g17260
malus_domestica MD13G1173400.v1.1
prunus_persica Prupe.1G141600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0421181
rosa_laevigata RLG00000007708
rosa_multiflora Rmu_sc0009290.1_g000009
rosa_roxburghii Rroxscaffold_5G00363480
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229800 Rh4BG232500 Rh4CG244100 Rh4DG228300
rosa_wichuraiana Rw4G020400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 550
AccIII TCCGGA 1 cut(s) 183
AciI CCGC 3 cut(s) 335, 350, 541
AclWI GGATC 3 cut(s) 254, 637, 650
AcoI YGGCCR 2 cut(s) 3, 7
AcsI RAATTY 1 cut(s) 299
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 2 cut(s) 12, 252
AjnI CCWGG 1 cut(s) 262
Alw26I GTCTC 3 cut(s) 635, 656, 690
AlwI GGATC 3 cut(s) 254, 637, 650
AlwNI CAGNNNCTG 2 cut(s) 269, 479
Aor13HI TCCGGA 1 cut(s) 183
AoxI GGCC 4 cut(s) 3, 7, 149, 201
ApeKI GCWGC 1 cut(s) 116
ApoI RAATTY 1 cut(s) 299
Asp700I GAANNNNTTC 1 cut(s) 289
AspLEI GCGC 2 cut(s) 406, 712
AspS9I GGNCC 1 cut(s) 615
AsuHPI GGTGA 2 cut(s) 303, 530
AvaII GGWCC 1 cut(s) 615
BamHI GGATCC 1 cut(s) 642
BbvCI CCTCAGC 1 cut(s) 472
BbvI GCAGC 1 cut(s) 128
BccI CCATC 1 cut(s) 415
BciT130I CCWGG 1 cut(s) 264
BciVI GTATCC 1 cut(s) 235
BcoDI GTCTC 3 cut(s) 635, 656, 690
BfaI CTAG 2 cut(s) 234, 557
BfoI RGCGCY 1 cut(s) 407
BfuI GTATCC 1 cut(s) 235
BisI GCNGC 2 cut(s) 117, 336
BlpI GCTNAGC 1 cut(s) 400
BlsI GCNGC 2 cut(s) 118, 337
Bme1390I CCNGG 1 cut(s) 264
Bme18I GGWCC 1 cut(s) 615
BmgT120I GGNCC 1 cut(s) 615
BmiI GGNNCC 2 cut(s) 286, 644
BmrFI CCNGG 1 cut(s) 264
BmrI ACTGGG 1 cut(s) 489
BmsI GCATC 1 cut(s) 128
BmuI ACTGGG 1 cut(s) 489
Bpu10I CCTNAGC 1 cut(s) 472
Bpu1102I GCTNAGC 1 cut(s) 400
BpuEI CTTGAG 2 cut(s) 190, 497
BsaJI CCNNGG 3 cut(s) 225, 263, 567
BsaWI WCCGGW 1 cut(s) 183
BsaXI ACNNNNNCTCC 2 cut(s) 475, 505
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 252
Bse118I RCCGGY 3 cut(s) 5, 9, 488
Bse1I ACTGG 1 cut(s) 484
BseAI TCCGGA 1 cut(s) 183
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 225, 263, 567
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 12, 252
BseMII CTCAG 3 cut(s) 261, 486, 603
BseNI ACTGG 1 cut(s) 484
BseX3I CGGCCG 1 cut(s) 7
BseXI GCAGC 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 4 cut(s) 5, 9, 151, 203
BsiEI CGRYCG 1 cut(s) 10
BsiSI CCGG 4 cut(s) 6, 10, 184, 489
BslFI GGGAC 1 cut(s) 280
BslI CCNNNNNNNGG 2 cut(s) 12, 252
BsmAI GTCTC 3 cut(s) 635, 656, 690
BsmFI GGGAC 1 cut(s) 280
BsnI GGCC 4 cut(s) 5, 9, 151, 203
Bsp13I TCCGGA 1 cut(s) 183
Bsp143I GATC 4 cut(s) 195, 259, 520, 642
Bsp1720I GCTNAGC 1 cut(s) 400
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 3 cut(s) 335, 350, 541
BspANI GGCC 4 cut(s) 5, 9, 151, 203
BspCNI CTCAG 3 cut(s) 262, 485, 602
BspEI TCCGGA 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 286, 644
BspPI GGATC 3 cut(s) 254, 637, 650
BsrFI RCCGGY 3 cut(s) 5, 9, 488
BsrI ACTGG 1 cut(s) 484
BssAI RCCGGY 3 cut(s) 5, 9, 488
BssECI CCNNGG 3 cut(s) 225, 263, 567
BssMI GATC 4 cut(s) 195, 259, 520, 642
BssT1I CCWWGG 2 cut(s) 225, 567
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 4 cut(s) 72, 281, 548, 684
Bst6I CTCTTC 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 7, 352
BstDEI CTNAG 5 cut(s) 76, 270, 400, 472, 589
BstDSI CCRYGG 1 cut(s) 225
BstF5I GGATG 1 cut(s) 192
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 2 cut(s) 406, 712
BstKTI GATC 4 cut(s) 198, 262, 523, 645
BstMAI GTCTC 3 cut(s) 635, 656, 690
BstMBI GATC 4 cut(s) 195, 259, 520, 642
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNI CCWGG 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 262
BstV1I GCAGC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 642
BstYI RGATCY 1 cut(s) 642
BstZI CGGCCG 1 cut(s) 7
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 4 cut(s) 5, 9, 151, 203
BtgI CCRYGG 1 cut(s) 225
BtsCI GGATG 1 cut(s) 192
BtsIMutI CAGTG 3 cut(s) 77, 286, 303
Cac8I GCNNGC 2 cut(s) 7, 352
CaiI CAGNNNCTG 2 cut(s) 269, 479
CfoI GCGC 2 cut(s) 406, 712
Cfr10I RCCGGY 3 cut(s) 5, 9, 488
Cfr13I GGNCC 1 cut(s) 615
Csp6I GTAC 1 cut(s) 256
CviAII CATG 2 cut(s) 133, 226
CviJI RGCY 6 cut(s) 5, 9, 43, 151, 203, 470
CviKI_1 RGCY 6 cut(s) 5, 9, 43, 151, 203, 470
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 5 cut(s) 76, 270, 400, 472, 589
DpnI GATC 4 cut(s) 197, 261, 522, 644
DpnII GATC 4 cut(s) 195, 259, 520, 642
EaeI YGGCCR 2 cut(s) 3, 7
EagI CGGCCG 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
EclXI CGGCCG 1 cut(s) 7
Eco130I CCWWGG 2 cut(s) 225, 567
Eco147I AGGCCT 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 615
Eco52I CGGCCG 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 262
EcoT14I CCWWGG 2 cut(s) 225, 567
EcoT22I ATGCAT 1 cut(s) 763
ErhI CCWWGG 2 cut(s) 225, 567
FaeI CATG 2 cut(s) 136, 229
FaqI GGGAC 1 cut(s) 280
FatI CATG 2 cut(s) 132, 225
FauI CCCGC 1 cut(s) 343
FauNDI CATATG 1 cut(s) 757
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 2 cut(s) 117, 336
FokI GGATG 1 cut(s) 199
FseI GGCCGGCC 1 cut(s) 9
Fsp4HI GCNGC 2 cut(s) 117, 336
FspBI CTAG 2 cut(s) 234, 557
GlaI GCGC 2 cut(s) 405, 711
GluI GCNGC 2 cut(s) 117, 336
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 4 cut(s) 5, 9, 151, 203
HapII CCGG 4 cut(s) 6, 10, 184, 489
HhaI GCGC 2 cut(s) 406, 712
Hin1II CATG 2 cut(s) 136, 229
Hin6I GCGC 2 cut(s) 404, 710
HinP1I GCGC 2 cut(s) 404, 710
HincII GTYRAC 3 cut(s) 24, 82, 551
HindII GTYRAC 3 cut(s) 24, 82, 551
HinfI GANTC 3 cut(s) 308, 561, 724
HpaI GTTAAC 1 cut(s) 82
HpaII CCGG 4 cut(s) 6, 10, 184, 489
HphI GGTGA 2 cut(s) 303, 530
Hpy166II GTNNAC 4 cut(s) 24, 82, 160, 551
Hpy188III TCNNGA 1 cut(s) 184
Hpy8I GTNNAC 4 cut(s) 24, 82, 160, 551
HpyAV CCTTC 1 cut(s) 737
HpyCH4III ACNGT 4 cut(s) 72, 281, 548, 684
HpyCH4V TGCA 2 cut(s) 500, 761
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 5 cut(s) 76, 270, 400, 472, 589
Hsp92II CATG 2 cut(s) 136, 229
HspAI GCGC 2 cut(s) 404, 710
Kpn2I TCCGGA 1 cut(s) 183
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
KspAI GTTAAC 1 cut(s) 82
Kzo9I GATC 4 cut(s) 195, 259, 520, 642
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 9 cut(s) 19, 23, 165, 197, 249, 276, 465, 502, 719
Lsp1109I GCAGC 1 cut(s) 128
LweI GCATC 1 cut(s) 128
MaeI CTAG 2 cut(s) 234, 557
MaeIII GTNAC 3 cut(s) 220, 229, 690
MalI GATC 4 cut(s) 197, 261, 522, 644
MboI GATC 4 cut(s) 195, 259, 520, 642
MboII GAAGA 4 cut(s) 157, 170, 402, 599
MflI RGATCY 1 cut(s) 642
MluCI AATT 3 cut(s) 210, 299, 624
MlyI GAGTC 2 cut(s) 570, 718
MnlI CCTC 6 cut(s) 141, 214, 325, 481, 598, 628
Mph1103I ATGCAT 1 cut(s) 763
MroI TCCGGA 1 cut(s) 183
MroNI GCCGGC 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 2 cut(s) 81, 766
MspI CCGG 4 cut(s) 6, 10, 184, 489
MspR9I CCNGG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 1 cut(s) 125
NaeI GCCGGC 1 cut(s) 7
NcoI CCATGG 1 cut(s) 225
NdeI CATATG 1 cut(s) 757
NdeII GATC 4 cut(s) 195, 259, 520, 642
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 136, 229
NlaIV GGNNCC 2 cut(s) 286, 644
NmuCI GTSAC 1 cut(s) 220
NsiI ATGCAT 1 cut(s) 763
PceI AGGCCT 1 cut(s) 151
PdiI GCCGGC 1 cut(s) 7
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 1 cut(s) 308
PkrI GCNGC 2 cut(s) 118, 337
PleI GAGTC 2 cut(s) 569, 718
PpsI GAGTC 2 cut(s) 569, 718
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 2 cut(s) 286, 644
PspPI GGNCC 1 cut(s) 615
PstNI CAGNNNCTG 2 cut(s) 269, 479
PsuI RGATCY 1 cut(s) 642
RigI GGCCGGCC 1 cut(s) 9
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
SalI GTCGAC 1 cut(s) 549
SaqAI TTAA 2 cut(s) 81, 766
SatI GCNGC 2 cut(s) 117, 336
Sau3AI GATC 4 cut(s) 195, 259, 520, 642
Sau96I GGNCC 1 cut(s) 615
SchI GAGTC 2 cut(s) 570, 718
ScrFI CCNGG 1 cut(s) 264
SetI ASST 4 cut(s) 98, 290, 620, 719
SfaNI GCATC 1 cut(s) 128
SinI GGWCC 1 cut(s) 615
SmlI CTYRAG 2 cut(s) 169, 512
SmoI CTYRAG 2 cut(s) 169, 512
Sse9I AATT 3 cut(s) 210, 299, 624
SseBI AGGCCT 1 cut(s) 151
SsiI CCGC 3 cut(s) 335, 350, 541
SspI AATATT 1 cut(s) 251
SspMI CTAG 2 cut(s) 234, 557
StuI AGGCCT 1 cut(s) 151
StyD4I CCNGG 1 cut(s) 262
StyI CCWWGG 2 cut(s) 225, 567
TaaI ACNGT 4 cut(s) 72, 281, 548, 684
TaqI TCGA 2 cut(s) 206, 550
TasI AATT 3 cut(s) 210, 299, 624
TauI GCSGC 1 cut(s) 338
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 2 cut(s) 81, 766
Tru9I TTAA 2 cut(s) 81, 766
TscAI CASTG 3 cut(s) 77, 286, 310
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 310, 402
TspGWI ACGGA 2 cut(s) 283, 678
TspRI CASTG 3 cut(s) 77, 286, 310
VpaK11BI GGWCC 1 cut(s) 615
XapI RAATTY 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 2 cut(s) 234, 557
Zsp2I ATGCAT 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.