RLG00000007708

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0017286
Physical Location & Seq
Forward (+)
20102418 .. 20104759
2342 bp
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UTR
Exon/CDS
Intron
RLM00000007708

Sequence Viewer

Length: 576 bp
ATGGGAACAACGAAGAGGCCTGTGGTTTACTTTTGCTTGAGTTTTCTTCTCCGGATGTTGTTGATCGTGGCCTCGACAATTAGTGCTGTGACCATGGTAACTAGCAAGGATACCCAATATTGGTACGATCCAGGGACTGTCGACTATGACAGTGGAACCTATTCCGTCAAATTCACCGATTCACCCTCATTCATGTACATCATCCGCTTAGCGCCATCTTCATCAATAATGTTGGTTCTCTTTGCGTTTTTTGATGTGTTGATATTGGGAGTGGTAGCCTCAGCAACTGGGAGTGCCGGTAGTGTTGCATATAGCATACTCAAGGGTGATCTTTGCTCTACTTACAACCGCTACTGTCGACATCTAGGAGTCGCCTTGGGGTTCGCCTTATTTGCCTCAGTTTTGCTTGTAGTTCTTCTATGGACCTCCCTAATTAGTCTCTACAAAAGGATCCCCAAACAAACCTTTGAGACACCACAACCACTTGCAGAGTCTCCGTTACAAACCCCACCACAAGCGCAGACCTTACAGACTCCACCACAGGCACAAGAAGGAACTAAAACATATGCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

192

Amino Acids

21.0

Weight (kDa)

8.73

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 13 - 68 1.4e-06 Casparian strip membrane protein domain
CASP_dom PF04535 73 - 135 1.4e-08 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017286)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g17260
malus_domestica MD13G1173400.v1.1
prunus_persica Prupe.1G141600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0421181
rosa_laevigata RLG00000007708
rosa_multiflora Rmu_sc0009290.1_g000009
rosa_roxburghii Rroxscaffold_5G00363480
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229800 Rh4BG232500 Rh4CG244100 Rh4DG228300
rosa_wichuraiana Rw4G020400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 141, 358
AccIII TCCGGA 1 cut(s) 51
AciI CCGC 2 cut(s) 205, 349
AclWI GGATC 3 cut(s) 122, 445, 458
AcsI RAATTY 1 cut(s) 170
AfaI GTAC 2 cut(s) 125, 197
AfiI CCNNNNNNNGG 1 cut(s) 120
AjnI CCWGG 1 cut(s) 130
Alw26I GTCTC 3 cut(s) 443, 464, 498
AlwI GGATC 3 cut(s) 122, 445, 458
AlwNI CAGNNNCTG 2 cut(s) 137, 287
Aor13HI TCCGGA 1 cut(s) 51
AoxI GGCC 2 cut(s) 17, 69
ApoI RAATTY 1 cut(s) 170
Asp700I GAANNNNTTC 1 cut(s) 160
AspLEI GCGC 2 cut(s) 214, 520
AspS9I GGNCC 1 cut(s) 423
AsuHPI GGTGA 3 cut(s) 166, 174, 338
AvaII GGWCC 1 cut(s) 423
BamHI GGATCC 1 cut(s) 450
BbvCI CCTCAGC 1 cut(s) 280
BccI CCATC 1 cut(s) 223
BciT130I CCWGG 1 cut(s) 132
BciVI GTATCC 1 cut(s) 103
BcoDI GTCTC 3 cut(s) 443, 464, 498
BfaI CTAG 2 cut(s) 102, 365
BfoI RGCGCY 1 cut(s) 215
BfuI GTATCC 1 cut(s) 103
BlpI GCTNAGC 1 cut(s) 208
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 423
BmiI GGNNCC 2 cut(s) 157, 452
BmrFI CCNGG 1 cut(s) 132
BmrI ACTGGG 1 cut(s) 297
BmuI ACTGGG 1 cut(s) 297
Bpu10I CCTNAGC 1 cut(s) 280
Bpu1102I GCTNAGC 1 cut(s) 208
BpuEI CTTGAG 2 cut(s) 58, 305
BsaJI CCNNGG 3 cut(s) 93, 131, 375
BsaWI WCCGGW 1 cut(s) 51
BsaXI ACNNNNNCTCC 2 cut(s) 283, 313
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 292
BseAI TCCGGA 1 cut(s) 51
BseBI CCWGG 1 cut(s) 132
BseDI CCNNGG 3 cut(s) 93, 131, 375
BseGI GGATG 2 cut(s) 60, 201
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMII CTCAG 2 cut(s) 294, 411
BseNI ACTGG 1 cut(s) 292
BshFI GGCC 2 cut(s) 19, 71
BsiSI CCGG 2 cut(s) 52, 297
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 120
BsmAI GTCTC 3 cut(s) 443, 464, 498
BsmFI GGGAC 1 cut(s) 148
BsnI GGCC 2 cut(s) 19, 71
Bsp13I TCCGGA 1 cut(s) 51
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 4 cut(s) 63, 127, 328, 450
Bsp1720I GCTNAGC 1 cut(s) 208
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 2 cut(s) 205, 349
BspANI GGCC 2 cut(s) 19, 71
BspCNI CTCAG 2 cut(s) 293, 410
BspEI TCCGGA 1 cut(s) 51
BspLI GGNNCC 2 cut(s) 157, 452
BspPI GGATC 3 cut(s) 122, 445, 458
BsrFI RCCGGY 1 cut(s) 296
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 1 cut(s) 292
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 3 cut(s) 93, 131, 375
BssMI GATC 4 cut(s) 63, 127, 328, 450
BssT1I CCWWGG 2 cut(s) 93, 375
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 3 cut(s) 139, 152, 356
Bst6I CTCTTC 1 cut(s) 8
BstAUI TGTACA 1 cut(s) 195
BstDEI CTNAG 3 cut(s) 208, 280, 397
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 2 cut(s) 60, 201
BstH2I RGCGCY 1 cut(s) 215
BstHHI GCGC 2 cut(s) 214, 520
BstKTI GATC 4 cut(s) 66, 130, 331, 453
BstMAI GTCTC 3 cut(s) 443, 464, 498
BstMBI GATC 4 cut(s) 63, 127, 328, 450
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 130
BstX2I RGATCY 1 cut(s) 450
BstYI RGATCY 1 cut(s) 450
BsuI GTATCC 1 cut(s) 103
BsuRI GGCC 2 cut(s) 19, 71
BtgI CCRYGG 1 cut(s) 93
BtsCI GGATG 2 cut(s) 60, 201
BtsIMutI CAGTG 1 cut(s) 157
CaiI CAGNNNCTG 2 cut(s) 137, 287
CfoI GCGC 2 cut(s) 214, 520
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 423
Csp6I GTAC 2 cut(s) 124, 196
CviAII CATG 2 cut(s) 94, 193
CviJI RGCY 3 cut(s) 19, 71, 278
CviKI_1 RGCY 3 cut(s) 19, 71, 278
CviQI GTAC 2 cut(s) 124, 196
DdeI CTNAG 3 cut(s) 208, 280, 397
DpnI GATC 4 cut(s) 65, 129, 330, 452
DpnII GATC 4 cut(s) 63, 127, 328, 450
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 93, 375
Eco147I AGGCCT 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 130
EcoT14I CCWWGG 2 cut(s) 93, 375
EcoT22I ATGCAT 1 cut(s) 571
ErhI CCWWGG 2 cut(s) 93, 375
FaeI CATG 2 cut(s) 97, 196
FaqI GGGAC 1 cut(s) 148
FatI CATG 2 cut(s) 93, 192
FauNDI CATATG 1 cut(s) 565
FblI GTMKAC 2 cut(s) 141, 358
FokI GGATG 2 cut(s) 67, 188
FspBI CTAG 2 cut(s) 102, 365
GlaI GCGC 2 cut(s) 213, 519
HaeII RGCGCY 1 cut(s) 215
HaeIII GGCC 2 cut(s) 19, 71
HapII CCGG 2 cut(s) 52, 297
HhaI GCGC 2 cut(s) 214, 520
Hin1II CATG 2 cut(s) 97, 196
Hin6I GCGC 2 cut(s) 212, 518
HinP1I GCGC 2 cut(s) 212, 518
HincII GTYRAC 2 cut(s) 142, 359
HindII GTYRAC 2 cut(s) 142, 359
HinfI GANTC 4 cut(s) 179, 369, 491, 532
HpaII CCGG 2 cut(s) 52, 297
HphI GGTGA 3 cut(s) 166, 174, 338
Hpy166II GTNNAC 3 cut(s) 28, 142, 359
Hpy188III TCNNGA 1 cut(s) 52
Hpy8I GTNNAC 3 cut(s) 28, 142, 359
HpyAV CCTTC 1 cut(s) 545
HpyCH4III ACNGT 3 cut(s) 139, 152, 356
HpyCH4V TGCA 3 cut(s) 308, 488, 569
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 3 cut(s) 208, 280, 397
Hsp92II CATG 2 cut(s) 97, 196
HspAI GCGC 2 cut(s) 212, 518
Kpn2I TCCGGA 1 cut(s) 51
Kzo9I GATC 4 cut(s) 63, 127, 328, 450
LpnPI CCDG 7 cut(s) 33, 65, 117, 144, 273, 310, 527
MaeI CTAG 2 cut(s) 102, 365
MaeIII GTNAC 3 cut(s) 88, 97, 498
MalI GATC 4 cut(s) 65, 129, 330, 452
MboI GATC 4 cut(s) 63, 127, 328, 450
MboII GAAGA 4 cut(s) 25, 38, 210, 407
MflI RGATCY 1 cut(s) 450
MluCI AATT 3 cut(s) 78, 170, 432
MlyI GAGTC 3 cut(s) 378, 500, 526
MnlI CCTC 6 cut(s) 9, 82, 196, 289, 406, 436
Mph1103I ATGCAT 1 cut(s) 571
MroI TCCGGA 1 cut(s) 51
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 1 cut(s) 574
MspI CCGG 2 cut(s) 52, 297
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 392
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 565
NdeII GATC 4 cut(s) 63, 127, 328, 450
NlaIII CATG 2 cut(s) 97, 196
NlaIV GGNNCC 2 cut(s) 157, 452
NmuCI GTSAC 1 cut(s) 88
NsiI ATGCAT 1 cut(s) 571
PceI AGGCCT 1 cut(s) 19
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 179
PleI GAGTC 3 cut(s) 377, 499, 526
PpsI GAGTC 3 cut(s) 377, 499, 526
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 157, 452
PspPI GGNCC 1 cut(s) 423
PstNI CAGNNNCTG 2 cut(s) 137, 287
PsuI RGATCY 1 cut(s) 450
RsaI GTAC 2 cut(s) 125, 197
RsaNI GTAC 2 cut(s) 124, 196
SalI GTCGAC 2 cut(s) 140, 357
SaqAI TTAA 1 cut(s) 574
Sau3AI GATC 4 cut(s) 63, 127, 328, 450
Sau96I GGNCC 1 cut(s) 423
SchI GAGTC 3 cut(s) 378, 500, 526
ScrFI CCNGG 1 cut(s) 132
SetI ASST 4 cut(s) 161, 428, 467, 527
SinI GGWCC 1 cut(s) 423
SmlI CTYRAG 2 cut(s) 37, 320
SmoI CTYRAG 2 cut(s) 37, 320
Sse9I AATT 3 cut(s) 78, 170, 432
SseBI AGGCCT 1 cut(s) 19
SsiI CCGC 2 cut(s) 205, 349
SspI AATATT 1 cut(s) 119
SspMI CTAG 2 cut(s) 102, 365
StuI AGGCCT 1 cut(s) 19
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 2 cut(s) 93, 375
TaaI ACNGT 3 cut(s) 139, 152, 356
TaqI TCGA 3 cut(s) 74, 141, 358
TasI AATT 3 cut(s) 78, 170, 432
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 1 cut(s) 574
Tru9I TTAA 1 cut(s) 574
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 88
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 2 cut(s) 181, 210
TspGWI ACGGA 2 cut(s) 154, 486
TspRI CASTG 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 423
XapI RAATTY 1 cut(s) 170
XmiI GTMKAC 2 cut(s) 141, 358
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 2 cut(s) 102, 365
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.