Rroxscaffold_5G00363480

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0017286
Physical Location & Seq
Reverse (-)
44692451 .. 44693519
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00363480.1

Sequence Viewer

Length: 543 bp
ATGGTAACTAGCAAGGATACCCAATATTGGTACGATCCAGGGACTGACTATGACAGTGGAACCTATTCCGTCAAATTCACTGATTCACCCTCATTCATATATTTTGTTGCGGCATTATCTGTGGCGGGCGTTTATGGTATCATTACCAAGTTATCATCTTGTTTAGTCATCTGCTTAGCGCCATCTTCATCAATGATGTTGGTTCTCTTTGCGTTTTTTGATGTGTTGATATTGGGAGTGGTAGCCTCAGCAACTGGGAGTGCCGGTAGTGTTGCATATAGCATACTCAAGGGTGATCTTTGCTCTACTTACACCTGCTACTGTCGACATCTAGGAGTCGCCTTGGGGTTCGCCTTATTTGCCTCAGTTTTGCTTATCGTTCTTCTATGGACCTCCCTAATTAGTCTCTACAAAAGGATCCCCAAACAAACCTTTGAGACACCACAACCACTTGCACAGTCTCCGTTACAAACCCCACCACAAGCGCAGACCTTACAGACTCCACCACAGGCACAAGAAGGAACTAAAACATATGCATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

180

Amino Acids

19.46

Weight (kDa)

6.51

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 1 - 124 3.7e-17 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017286)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g17260
malus_domestica MD13G1173400.v1.1
prunus_persica Prupe.1G141600_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0421181
rosa_laevigata RLG00000007708
rosa_multiflora Rmu_sc0009290.1_g000009
rosa_roxburghii Rroxscaffold_5G00363480
rosa_rugosa Rorug04G0170000
rosa_samantha Rh4AG229800 Rh4BG232500 Rh4CG244100 Rh4DG228300
rosa_wichuraiana Rw4G020400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 323
AccI GTMKAC 1 cut(s) 325
AciI CCGC 2 cut(s) 110, 125
AclWI GGATC 3 cut(s) 29, 412, 425
AcsI RAATTY 1 cut(s) 74
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 1 cut(s) 27
AjnI CCWGG 1 cut(s) 37
Alw26I GTCTC 3 cut(s) 410, 431, 465
AlwI GGATC 3 cut(s) 29, 412, 425
AlwNI CAGNNNCTG 2 cut(s) 44, 254
ApoI RAATTY 1 cut(s) 74
Asp700I GAANNNNTTC 1 cut(s) 64
AspLEI GCGC 2 cut(s) 181, 487
AspS9I GGNCC 1 cut(s) 390
AsuHPI GGTGA 2 cut(s) 78, 305
AvaII GGWCC 1 cut(s) 390
BamHI GGATCC 1 cut(s) 417
BbvCI CCTCAGC 1 cut(s) 247
BccI CCATC 1 cut(s) 190
BciT130I CCWGG 1 cut(s) 39
BciVI GTATCC 1 cut(s) 10
BcoDI GTCTC 3 cut(s) 410, 431, 465
BfaI CTAG 2 cut(s) 9, 332
BfoI RGCGCY 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 323
BfuI GTATCC 1 cut(s) 10
BisI GCNGC 1 cut(s) 111
BlpI GCTNAGC 1 cut(s) 175
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 39
Bme18I GGWCC 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 390
BmiI GGNNCC 2 cut(s) 61, 419
BmrFI CCNGG 1 cut(s) 39
BmrI ACTGGG 1 cut(s) 264
BmuI ACTGGG 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 247
Bpu1102I GCTNAGC 1 cut(s) 175
BpuEI CTTGAG 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 38, 342
BsaXI ACNNNNNCTCC 2 cut(s) 250, 280
Bsc4I CCNNNNNNNGG 1 cut(s) 27
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 2 cut(s) 38, 342
BseLI CCNNNNNNNGG 1 cut(s) 27
BseMII CTCAG 2 cut(s) 261, 378
BseNI ACTGG 1 cut(s) 259
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 1 cut(s) 27
BsmAI GTCTC 3 cut(s) 410, 431, 465
BsmFI GGGAC 1 cut(s) 55
Bsp143I GATC 3 cut(s) 34, 295, 417
Bsp1720I GCTNAGC 1 cut(s) 175
BspACI CCGC 2 cut(s) 110, 125
BspCNI CTCAG 2 cut(s) 260, 377
BspLI GGNNCC 2 cut(s) 61, 419
BspMI ACCTGC 1 cut(s) 323
BspPI GGATC 3 cut(s) 29, 412, 425
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 38, 342
BssMI GATC 3 cut(s) 34, 295, 417
BssT1I CCWWGG 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 3 cut(s) 56, 323, 459
BstC8I GCNNGC 1 cut(s) 127
BstDEI CTNAG 3 cut(s) 175, 247, 364
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 2 cut(s) 181, 487
BstKTI GATC 3 cut(s) 37, 298, 420
BstMAI GTCTC 3 cut(s) 410, 431, 465
BstMBI GATC 3 cut(s) 34, 295, 417
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 1 cut(s) 39
BstSCI CCNGG 1 cut(s) 37
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
BsuI GTATCC 1 cut(s) 10
BtsIMutI CAGTG 2 cut(s) 61, 78
BveI ACCTGC 1 cut(s) 323
Cac8I GCNNGC 1 cut(s) 127
CaiI CAGNNNCTG 2 cut(s) 44, 254
CfoI GCGC 2 cut(s) 181, 487
Cfr10I RCCGGY 1 cut(s) 263
Cfr13I GGNCC 1 cut(s) 390
Csp6I GTAC 1 cut(s) 31
CviJI RGCY 1 cut(s) 245
CviKI_1 RGCY 1 cut(s) 245
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 3 cut(s) 175, 247, 364
DpnI GATC 3 cut(s) 36, 297, 419
DpnII GATC 3 cut(s) 34, 295, 417
Eco130I CCWWGG 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 390
EcoRII CCWGG 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 342
EcoT22I ATGCAT 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 342
FaqI GGGAC 1 cut(s) 55
FauI CCCGC 1 cut(s) 118
FauNDI CATATG 1 cut(s) 532
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 2 cut(s) 9, 332
GlaI GCGC 2 cut(s) 180, 486
GluI GCNGC 1 cut(s) 111
HaeII RGCGCY 1 cut(s) 182
HapII CCGG 1 cut(s) 264
HhaI GCGC 2 cut(s) 181, 487
Hin6I GCGC 2 cut(s) 179, 485
HinP1I GCGC 2 cut(s) 179, 485
HincII GTYRAC 1 cut(s) 326
HindII GTYRAC 1 cut(s) 326
HinfI GANTC 3 cut(s) 83, 336, 499
HpaII CCGG 1 cut(s) 264
HphI GGTGA 2 cut(s) 78, 305
Hpy166II GTNNAC 1 cut(s) 326
Hpy8I GTNNAC 1 cut(s) 326
HpyAV CCTTC 1 cut(s) 512
HpyCH4III ACNGT 3 cut(s) 56, 323, 459
HpyCH4V TGCA 3 cut(s) 275, 455, 536
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 3 cut(s) 175, 247, 364
HspAI GCGC 2 cut(s) 179, 485
Kzo9I GATC 3 cut(s) 34, 295, 417
LpnPI CCDG 6 cut(s) 24, 51, 240, 277, 328, 494
MaeI CTAG 2 cut(s) 9, 332
MaeIII GTNAC 2 cut(s) 4, 465
MalI GATC 3 cut(s) 36, 297, 419
MboI GATC 3 cut(s) 34, 295, 417
MboII GAAGA 2 cut(s) 177, 374
MflI RGATCY 1 cut(s) 417
MluCI AATT 2 cut(s) 74, 399
MlyI GAGTC 2 cut(s) 345, 493
MnlI CCTC 4 cut(s) 100, 256, 373, 403
Mph1103I ATGCAT 1 cut(s) 538
MroXI GAANNNNTTC 1 cut(s) 64
MseI TTAA 1 cut(s) 541
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 39
MvaI CCWGG 1 cut(s) 39
MwoI GCNNNNNNNGC 1 cut(s) 359
NdeI CATATG 1 cut(s) 532
NdeII GATC 3 cut(s) 34, 295, 417
NlaIV GGNNCC 2 cut(s) 61, 419
NsiI ATGCAT 1 cut(s) 538
PaqCI CACCTGC 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 1 cut(s) 112
PleI GAGTC 2 cut(s) 344, 493
PpsI GAGTC 2 cut(s) 344, 493
Psp6I CCWGG 1 cut(s) 37
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 2 cut(s) 61, 419
PspPI GGNCC 1 cut(s) 390
PstNI CAGNNNCTG 2 cut(s) 44, 254
PsuI RGATCY 1 cut(s) 417
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
SalI GTCGAC 1 cut(s) 324
SaqAI TTAA 1 cut(s) 541
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 3 cut(s) 34, 295, 417
Sau96I GGNCC 1 cut(s) 390
SchI GAGTC 2 cut(s) 345, 493
ScrFI CCNGG 1 cut(s) 39
SetI ASST 5 cut(s) 65, 317, 395, 434, 494
SinI GGWCC 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 2 cut(s) 74, 399
SsiI CCGC 2 cut(s) 110, 125
SspI AATATT 1 cut(s) 26
SspMI CTAG 2 cut(s) 9, 332
StyD4I CCNGG 1 cut(s) 37
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 3 cut(s) 56, 323, 459
TaqI TCGA 1 cut(s) 325
TasI AATT 2 cut(s) 74, 399
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 541
Tru9I TTAA 1 cut(s) 541
TscAI CASTG 2 cut(s) 61, 85
TspDTI ATGAA 2 cut(s) 85, 177
TspGWI ACGGA 2 cut(s) 58, 453
TspRI CASTG 2 cut(s) 61, 85
VpaK11BI GGWCC 1 cut(s) 390
XapI RAATTY 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 64
XspI CTAG 2 cut(s) 9, 332
Zsp2I ATGCAT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.