FvH4_4g19570

pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015910
Physical Location & Seq
Reverse (-)
23190825 .. 23195113
4289 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19570.t1

Sequence Viewer

Length: 735 bp
ATGCAATCACTCCAGCCGACTGCAGCCCCATCGCCACCACCAGCTGCCGTGGATCCAGTTGTTAAACAGCTATGTAACAACACCAACCACTCGGATCTTTGCATTTCATCCCTCATCCCTTTCCTTTCAGCACAAGGAAAGACATCTCTAGATGCCGTAACCGTACTCAAAATGTTGATAAAGGCTTGTTCCATACAGGCAGAGCTGGCCCTGGCAGTGGCATCAAAACTCTCCACAGAGCCGAAGAATGCGCACCCGACGGCCATGGCCATCATGGATTGCAAGGATATGTATAGCGATGTGTTGGACGGCCTTCAAACCGCAACGGATGCAATTGCGAAAAAAGACATGAGCACTGTTAAGAGTATGCTTACCGGGGTAATTAGTGATGCTGTGACATGTGAGGATGGTTTTGCAGGACAAAAATCACCACTTGGTGATTTTGATGATAAACTTCGTAAGATGGGCGACAATTGCCTTGCCGTTGCTTTTTGGATAAAGTGTCTGGAGGAAAATGTAGCAGATAATAGAGAGCTCCATTCCCATGCTTTACCCAAAACTAGTCCAAACTTCCCCAAACCCCTCTCACCAGCATTTACTTCAGCTACCCTCTTCACCCACCTAGTAACCCTAGCTCCCTCCACCTCCAGGCTTCACAGCCCGTCTCCAGCTTCATCAAGGAGCAATGCGGTGTTCTTAATGACTTGTATTGGACTTCATCCCTTTGTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

245

Amino Acids

25.97

Weight (kDa)

6.36

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 20 - 162 2.1e-24 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G36659
fragaria_vesca FvH4_4g19570
malus_domestica MD16G1155100.v1.1
prunus_persica Prupe.1G118800_v2.0.a1
pyrus_communis pycom13g13250
rosa_chinensis RchiOBHm_Chr4g0424831
rosa_laevigata RLG00000007417
rosa_multiflora Rmu_sc0003608.1_g000023
rosa_roxburghii Rroxscaffold_5G00367050
rosa_rugosa Rorug04G0199400
rosa_samantha Rh4AG257200 Rh4BG262500 Rh4CG273500 Rh4DG258300
rosa_wichuraiana Rw4G022190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 252
AciI CCGC 2 cut(s) 321, 689
AclWI GGATC 3 cut(s) 47, 60, 102
AcoI YGGCCR 2 cut(s) 261, 267
AcuI CTGAAG 1 cut(s) 585
AdeI CACNNNGTG 1 cut(s) 437
AfaI GTAC 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 217, 648
AflIII ACRYGT 1 cut(s) 398
AgsI TTSAA 1 cut(s) 317
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 2 cut(s) 210, 647
AluBI AGCT 7 cut(s) 44, 70, 205, 535, 605, 635, 671
AluI AGCT 7 cut(s) 44, 70, 205, 535, 605, 635, 671
Alw21I GWGCWC 2 cut(s) 356, 537
Alw26I GTCTC 1 cut(s) 669
AlwI GGATC 3 cut(s) 47, 60, 102
AoxI GGCC 4 cut(s) 207, 261, 267, 310
ApeKI GCWGC 2 cut(s) 23, 44
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 4 cut(s) 420, 449, 579, 607
BalI TGGCCA 1 cut(s) 269
BamHI GGATCC 1 cut(s) 52
BanII GRGCYC 1 cut(s) 537
Bbv12I GWGCWC 2 cut(s) 356, 537
BbvI GCAGC 2 cut(s) 31, 35
BccI CCATC 4 cut(s) 37, 278, 401, 457
BceAI ACGGC 5 cut(s) 32, 140, 276, 325, 467
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 2 cut(s) 212, 649
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 669
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 4 cut(s) 149, 561, 623, 632
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 2 cut(s) 24, 45
BlsI GCNGC 2 cut(s) 25, 46
Bme1390I CCNGG 3 cut(s) 212, 376, 649
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 3 cut(s) 212, 376, 649
BmsI GCATC 4 cut(s) 142, 230, 319, 379
BpmI CTGGAG 3 cut(s) 527, 631, 651
BpuMI CCSGG 1 cut(s) 376
BsaJI CCNNGG 4 cut(s) 48, 210, 264, 375
BsaXI ACNNNNNCTCC 2 cut(s) 619, 649
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 648
Bse1I ACTGG 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 691
BseBI CCWGG 2 cut(s) 212, 649
BseDI CCNNGG 4 cut(s) 48, 210, 264, 375
BseGI GGATG 5 cut(s) 107, 114, 334, 412, 718
BseLI CCNNNNNNNGG 2 cut(s) 217, 648
BseMI GCAATG 1 cut(s) 691
BseNI ACTGG 1 cut(s) 56
BseXI GCAGC 2 cut(s) 31, 35
BshFI GGCC 4 cut(s) 209, 263, 269, 312
BsiHKAI GWGCWC 2 cut(s) 356, 537
BsiSI CCGG 1 cut(s) 375
BslI CCNNNNNNNGG 2 cut(s) 217, 648
BsmAI GTCTC 1 cut(s) 669
BsmBI CGTCTC 1 cut(s) 669
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 4 cut(s) 209, 263, 269, 312
Bsp1286I GDGCHC 2 cut(s) 356, 537
Bsp143I GATC 2 cut(s) 52, 94
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 2 cut(s) 321, 689
BspANI GGCC 4 cut(s) 209, 263, 269, 312
BspLI GGNNCC 1 cut(s) 54
BspMAI CTGCAG 1 cut(s) 25
BspPI GGATC 3 cut(s) 47, 60, 102
BsrDI GCAATG 1 cut(s) 691
BsrI ACTGG 1 cut(s) 56
BssECI CCNNGG 4 cut(s) 48, 210, 264, 375
BssMI GATC 2 cut(s) 52, 94
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 2 cut(s) 212, 649
Bst4CI ACNGT 2 cut(s) 163, 358
Bst6I CTCTTC 1 cut(s) 617
BstAPI GCANNNNNTGC 1 cut(s) 329
BstC8I GCNNGC 1 cut(s) 207
BstDSI CCRYGG 2 cut(s) 48, 264
BstF5I GGATG 5 cut(s) 107, 114, 334, 412, 718
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 2 cut(s) 55, 97
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 2 cut(s) 52, 94
BstMWI GCNNNNNNNGC 3 cut(s) 206, 329, 474
BstNI CCWGG 2 cut(s) 212, 649
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 3 cut(s) 210, 374, 647
BstSFI CTRYAG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 31, 35
BstX2I RGATCY 2 cut(s) 52, 94
BstYI RGATCY 2 cut(s) 52, 94
BsuRI GGCC 4 cut(s) 209, 263, 269, 312
BtgI CCRYGG 2 cut(s) 48, 264
BtgZI GCGATG 2 cut(s) 15, 312
BtsCI GGATG 5 cut(s) 107, 114, 334, 412, 718
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 2 cut(s) 222, 354
Cac8I GCNNGC 1 cut(s) 207
CfoI GCGC 1 cut(s) 253
Cfr13I GGNCC 1 cut(s) 208
Csp6I GTAC 1 cut(s) 164
CviAII CATG 5 cut(s) 265, 274, 349, 399, 545
CviQI GTAC 1 cut(s) 164
DpnI GATC 2 cut(s) 54, 96
DpnII GATC 2 cut(s) 52, 94
DraIII CACNNNGTG 1 cut(s) 437
EaeI YGGCCR 2 cut(s) 261, 267
Eam1104I CTCTTC 1 cut(s) 617
EarI CTCTTC 1 cut(s) 617
Ecl136II GAGCTC 1 cut(s) 535
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 537
Eco53kI GAGCTC 1 cut(s) 535
Eco57I CTGAAG 1 cut(s) 585
EcoICRI GAGCTC 1 cut(s) 535
EcoRII CCWGG 2 cut(s) 210, 647
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 264
Esp3I CGTCTC 1 cut(s) 669
FaeI CATG 5 cut(s) 268, 277, 352, 402, 548
FatI CATG 5 cut(s) 264, 273, 348, 398, 544
Fnu4HI GCNGC 2 cut(s) 24, 45
FokI GGATG 5 cut(s) 94, 101, 341, 419, 705
FriOI GRGCYC 1 cut(s) 537
Fsp4HI GCNGC 2 cut(s) 24, 45
FspAI RTGCGCAY 1 cut(s) 252
FspBI CTAG 4 cut(s) 149, 561, 623, 632
FspI TGCGCA 1 cut(s) 252
GlaI GCGC 1 cut(s) 252
GluI GCNGC 2 cut(s) 24, 45
GsuI CTGGAG 3 cut(s) 527, 631, 651
HaeIII GGCC 4 cut(s) 209, 263, 269, 312
HapII CCGG 1 cut(s) 375
HhaI GCGC 1 cut(s) 253
Hin1II CATG 5 cut(s) 268, 277, 352, 402, 548
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HpaII CCGG 1 cut(s) 375
HphI GGTGA 4 cut(s) 420, 449, 579, 607
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 2 cut(s) 149, 506
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 323
HpyCH4III ACNGT 2 cut(s) 163, 358
HpyCH4V TGCA 6 cut(s) 4, 23, 102, 282, 332, 416
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 329, 474
Hsp92II CATG 5 cut(s) 268, 277, 352, 402, 548
HspAI GCGC 1 cut(s) 251
Kzo9I GATC 2 cut(s) 52, 94
LmnI GCTCC 3 cut(s) 540, 640, 681
Lsp1109I GCAGC 2 cut(s) 31, 35
LweI GCATC 4 cut(s) 142, 230, 319, 379
MaeI CTAG 4 cut(s) 149, 561, 623, 632
MaeIII GTNAC 4 cut(s) 74, 157, 394, 625
MalI GATC 2 cut(s) 54, 96
MboI GATC 2 cut(s) 52, 94
MboII GAAGA 2 cut(s) 256, 604
MfeI CAATTG 2 cut(s) 333, 472
MflI RGATCY 2 cut(s) 52, 94
MhlI GDGCHC 2 cut(s) 356, 537
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 3 cut(s) 333, 381, 472
MluNI TGGCCA 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 7 cut(s) 122, 397, 502, 593, 620, 649, 655
Mox20I TGGCCA 1 cut(s) 269
MscI TGGCCA 1 cut(s) 269
MseI TTAA 3 cut(s) 63, 360, 698
MslI CAYNNNNRTG 1 cut(s) 543
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 3 cut(s) 212, 376, 649
MunI CAATTG 2 cut(s) 333, 472
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 2 cut(s) 212, 649
MwoI GCNNNNNNNGC 3 cut(s) 206, 329, 474
NciI CCSGG 1 cut(s) 376
NcoI CCATGG 1 cut(s) 264
NdeII GATC 2 cut(s) 52, 94
NlaIII CATG 5 cut(s) 268, 277, 352, 402, 548
NlaIV GGNNCC 1 cut(s) 54
NmuCI GTSAC 1 cut(s) 394
NsbI TGCGCA 1 cut(s) 252
NspI RCATGY 1 cut(s) 402
PciI ACATGT 1 cut(s) 398
PctI GAATGC 1 cut(s) 253
PkrI GCNGC 2 cut(s) 25, 46
PscI ACATGT 1 cut(s) 398
Psp124BI GAGCTC 1 cut(s) 537
Psp6I CCWGG 2 cut(s) 210, 647
PspGI CCWGG 2 cut(s) 210, 647
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 1 cut(s) 208
PstI CTGCAG 1 cut(s) 25
PsuI RGATCY 2 cut(s) 52, 94
PvuII CAGCTG 1 cut(s) 44
RsaI GTAC 1 cut(s) 165
RsaNI GTAC 1 cut(s) 164
RseI CAYNNNNRTG 1 cut(s) 543
SacI GAGCTC 1 cut(s) 537
SaqAI TTAA 3 cut(s) 63, 360, 698
SatI GCNGC 2 cut(s) 24, 45
Sau3AI GATC 2 cut(s) 52, 94
Sau96I GGNCC 1 cut(s) 208
ScrFI CCNGG 3 cut(s) 212, 376, 649
SduI GDGCHC 2 cut(s) 356, 537
SetI ASST 9 cut(s) 46, 72, 207, 537, 607, 624, 637, 647, 673
SfaNI GCATC 4 cut(s) 142, 230, 319, 379
SfcI CTRYAG 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 543
SpeI ACTAGT 1 cut(s) 560
Sse9I AATT 3 cut(s) 333, 381, 472
SsiI CCGC 2 cut(s) 321, 689
SspMI CTAG 4 cut(s) 149, 561, 623, 632
SstI GAGCTC 1 cut(s) 537
StyD4I CCNGG 3 cut(s) 210, 374, 647
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 163, 358
TasI AATT 3 cut(s) 333, 381, 472
Tru1I TTAA 3 cut(s) 63, 360, 698
Tru9I TTAA 3 cut(s) 63, 360, 698
TscAI CASTG 2 cut(s) 222, 361
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 2 cut(s) 23, 44
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 3 cut(s) 96, 663, 707
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 2 cut(s) 222, 361
XbaI TCTAGA 1 cut(s) 148
XceI RCATGY 1 cut(s) 402
XcmI CCANNNNNNNNNTGG 1 cut(s) 271
XspI CTAG 4 cut(s) 149, 561, 623, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.