MD16G1155100.v1.1

pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015910
Physical Location & Seq
Forward (+)
12294701 .. 12296076
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1155100.v1.1.491

Sequence Viewer

Length: 954 bp
ATGCTTAAAATTATCTCTCAACAGCCTCCCGTTCTTTCATTTTCTCCTCTTTTACTTCCTTCCACCCTGCAAGCAAATTCAAGAACAACAATGGATCCAAAGTGCTATACTCAAACGCCTCTGGTCATCTTATTCTTCTTCCTTTTCGGCTTCAACTCCGTGCAAGCCGTATGCAAGCCCCGGGACTCGAAACTGAGGTACCTAACTGTTCTCTCTCCAGCACCTAGTTCTCCTGACTCGCAGGCGTCTGTGCCTCCTAGTAAGCCACCGGCGGGTAAAGTCTTCCCTTCATCGCGGCCAGCAGGCCCTCCACCAACCCGTAGCCCTACTCCCGCCCCAACTCCCACCCCAAACCCTCCTTCACAGCCTCCACAAGTATCACCTCCTCCTGACAATTCTATTTCTCCATCTTCCTCCTCATCCTTTTTTCGCCGTCCTTTTGACAGACCGCGCGGAAACCTCTTTCCGCAGCCTTTTGTTCCCAACACGGCCTCATCATCAACCCCACCTAACCCGGCAATCCAAGAACTATGCAAAAACACAGACTACCCTGATCTTTGCATCTCATCCCTGACCCCTTTCCAACTACCCAAGGTTGACCCCATATCCGCGCTTGAGTTGGTAATCAAGGCTTGCACCGCACACGCCCAGATGTCTCTAGCCGCAGCCTCACAACTTGTGACGACGCCTAACACTGCGCGTGGCACGGTTGCTGCACTCAGCGATTGCAAGGAAATGTACTCTGATGCCTTGGACGGCCTTCAGAGCGCCATTGATGCAATCCAGAGCCGCGACATGGGCACGATTAATAGCATGCTTAGCGGGGTGGTGACCGATGCTGTGACGTGTGGGGATGGGTTTGAAGGACAGCAATCTCCGCTTGGTGATTACGATGACAAGCTTCATAAAATGGCCAGCAATGGCCTTGCCATTGCTTCTTTGATCAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

318

Amino Acids

33.55

Weight (kDa)

8.14

Isoelectric Point (pI)

66.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 173 - 311 1.1e-29 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G36659
fragaria_vesca FvH4_4g19570
malus_domestica MD16G1155100.v1.1
prunus_persica Prupe.1G118800_v2.0.a1
pyrus_communis pycom13g13250
rosa_chinensis RchiOBHm_Chr4g0424831
rosa_laevigata RLG00000007417
rosa_multiflora Rmu_sc0003608.1_g000023
rosa_roxburghii Rroxscaffold_5G00367050
rosa_rugosa Rorug04G0199400
rosa_samantha Rh4AG257200 Rh4BG262500 Rh4CG273500 Rh4DG258300
rosa_wichuraiana Rw4G022190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 198
AccB1I GGYRCC 1 cut(s) 198
AccII CGCG 6 cut(s) 295, 451, 453, 611, 700, 792
AclWI GGATC 2 cut(s) 89, 102
AcoI YGGCCR 2 cut(s) 296, 912
AcsI RAATTY 1 cut(s) 76
AcuI CTGAAG 1 cut(s) 746
AcyI GRCGYC 2 cut(s) 245, 686
AfaI GTAC 2 cut(s) 200, 740
AfiI CCNNNNNNNGG 2 cut(s) 272, 796
AflIII ACRYGT 1 cut(s) 845
AgsI TTSAA 3 cut(s) 81, 154, 863
AjiI CACGTC 1 cut(s) 846
AjuI GAANNNNNNNTTGG 2 cut(s) 343, 375
AluBI AGCT 1 cut(s) 901
AluI AGCT 1 cut(s) 901
Alw26I GTCTC 1 cut(s) 660
AlwI GGATC 2 cut(s) 89, 102
Ama87I CYCGRG 1 cut(s) 180
AoxI GGCC 6 cut(s) 296, 304, 489, 757, 912, 922
ApeKI GCWGC 3 cut(s) 469, 665, 713
ApoI RAATTY 1 cut(s) 76
AseI ATTAAT 1 cut(s) 807
Asp718I GGTACC 1 cut(s) 198
AspLEI GCGC 4 cut(s) 453, 613, 700, 770
AspS9I GGNCC 1 cut(s) 305
AsuC2I CCSGG 3 cut(s) 181, 182, 515
AsuHPI GGTGA 3 cut(s) 372, 841, 896
AvaI CYCGRG 1 cut(s) 180
BaeGI GKGCMC 1 cut(s) 803
BaeI ACNNNNGTAYC 2 cut(s) 182, 215
BalI TGGCCA 1 cut(s) 914
BamHI GGATCC 1 cut(s) 94
BanI GGYRCC 1 cut(s) 198
BbsI GAAGAC 1 cut(s) 274
BbvI GCAGC 3 cut(s) 481, 677, 700
BccI CCATC 2 cut(s) 415, 848
BceAI ACGGC 4 cut(s) 152, 417, 504, 772
BclI TGATCA 1 cut(s) 942
BcnI CCSGG 3 cut(s) 181, 182, 515
BcoDI GTCTC 1 cut(s) 660
BfaI CTAG 3 cut(s) 225, 258, 659
BfoI RGCGCY 1 cut(s) 771
BisI GCNGC 6 cut(s) 296, 470, 663, 666, 714, 790
BlpI GCTNAGC 1 cut(s) 818
BlsI GCNGC 6 cut(s) 297, 471, 664, 667, 715, 791
Bme1390I CCNGG 3 cut(s) 181, 182, 515
BmeT110I CYCGRG 1 cut(s) 180
BmgBI CACGTC 1 cut(s) 846
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 2 cut(s) 96, 200
BmrFI CCNGG 3 cut(s) 181, 182, 515
BmsI GCATC 4 cut(s) 570, 736, 766, 826
BpiI GAAGAC 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 201
Bpu1102I GCTNAGC 1 cut(s) 818
BpuEI CTTGAG 1 cut(s) 635
BpuMI CCSGG 3 cut(s) 181, 182, 515
BsaHI GRCGYC 2 cut(s) 245, 686
BsaJI CCNNGG 4 cut(s) 179, 180, 591, 750
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 796
Bse118I RCCGGY 1 cut(s) 268
Bse3DI GCAATG 2 cut(s) 925, 930
BseDI CCNNGG 4 cut(s) 179, 180, 591, 750
BseGI GGATG 3 cut(s) 419, 566, 859
BseLI CCNNNNNNNGG 2 cut(s) 272, 796
BseMI GCAATG 2 cut(s) 925, 930
BseMII CTCAG 2 cut(s) 185, 733
BseRI GAGGAG 3 cut(s) 36, 375, 406
BseSI GKGCMC 1 cut(s) 803
BseXI GCAGC 3 cut(s) 481, 677, 700
BsgI GTGCAG 1 cut(s) 699
Bsh1236I CGCG 6 cut(s) 295, 451, 453, 611, 700, 792
BshFI GGCC 6 cut(s) 298, 306, 491, 759, 914, 924
BshNI GGYRCC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 180
BsiSI CCGG 3 cut(s) 181, 269, 515
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 2 cut(s) 272, 796
BsmAI GTCTC 1 cut(s) 660
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 6 cut(s) 298, 306, 491, 759, 914, 924
BsoBI CYCGRG 1 cut(s) 180
Bsp1286I GDGCHC 1 cut(s) 803
Bsp143I GATC 3 cut(s) 94, 553, 942
Bsp1720I GCTNAGC 1 cut(s) 818
BspANI GGCC 6 cut(s) 298, 306, 491, 759, 914, 924
BspCNI CTCAG 2 cut(s) 186, 732
BspFNI CGCG 6 cut(s) 295, 451, 453, 611, 700, 792
BspLI GGNNCC 2 cut(s) 96, 200
BspPI GGATC 2 cut(s) 89, 102
BspT107I GGYRCC 1 cut(s) 198
BsrDI GCAATG 2 cut(s) 925, 930
BsrFI RCCGGY 1 cut(s) 268
BssAI RCCGGY 1 cut(s) 268
BssECI CCNNGG 4 cut(s) 179, 180, 591, 750
BssMI GATC 3 cut(s) 94, 553, 942
BssNI GRCGYC 2 cut(s) 245, 686
BssT1I CCWWGG 2 cut(s) 591, 750
Bst4CI ACNGT 2 cut(s) 208, 709
BstACI GRCGYC 2 cut(s) 245, 686
BstC8I GCNNGC 9 cut(s) 72, 165, 176, 243, 300, 304, 634, 815, 916
BstDEI CTNAG 3 cut(s) 194, 719, 818
BstEII GGTNACC 1 cut(s) 829
BstF5I GGATG 3 cut(s) 419, 566, 859
BstFNI CGCG 6 cut(s) 295, 451, 453, 611, 700, 792
BstH2I RGCGCY 1 cut(s) 771
BstHHI GCGC 4 cut(s) 453, 613, 700, 770
BstKTI GATC 3 cut(s) 97, 556, 945
BstMAI GTCTC 1 cut(s) 660
BstMBI GATC 3 cut(s) 94, 553, 942
BstMWI GCNNNNNNNGC 6 cut(s) 638, 765, 776, 798, 819, 877
BstNSI RCATGY 1 cut(s) 817
BstPI GGTNACC 1 cut(s) 829
BstSCI CCNGG 3 cut(s) 179, 180, 513
BstSLI GKGCMC 1 cut(s) 803
BstUI CGCG 6 cut(s) 295, 451, 453, 611, 700, 792
BstV1I GCAGC 3 cut(s) 481, 677, 700
BstV2I GAAGAC 1 cut(s) 274
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuRI GGCC 6 cut(s) 298, 306, 491, 759, 914, 924
BtgZI GCGATG 1 cut(s) 276
BtrI CACGTC 1 cut(s) 846
BtsCI GGATG 3 cut(s) 419, 566, 859
BtsI GCAGTG 1 cut(s) 693
BtsIMutI CAGTG 1 cut(s) 693
Cac8I GCNNGC 9 cut(s) 72, 165, 176, 243, 300, 304, 634, 815, 916
CfoI GCGC 4 cut(s) 453, 613, 700, 770
Cfr10I RCCGGY 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 305
Cfr9I CCCGGG 1 cut(s) 180
CseI GACGC 2 cut(s) 234, 694
Csp6I GTAC 2 cut(s) 199, 739
CviAII CATG 2 cut(s) 796, 814
CviQI GTAC 2 cut(s) 199, 739
DdeI CTNAG 3 cut(s) 194, 719, 818
DpnI GATC 3 cut(s) 96, 555, 944
DpnII GATC 3 cut(s) 94, 553, 942
EaeI YGGCCR 2 cut(s) 296, 912
Eco130I CCWWGG 2 cut(s) 591, 750
Eco57I CTGAAG 1 cut(s) 746
Eco88I CYCGRG 1 cut(s) 180
Eco91I GGTNACC 1 cut(s) 829
EcoO109I RGGNCCY 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 829
EcoT14I CCWWGG 2 cut(s) 591, 750
ErhI CCWWGG 2 cut(s) 591, 750
FaeI CATG 2 cut(s) 799, 817
FaiI YATR 7 cut(s) 108, 172, 532, 605, 797, 815, 906
FaqI GGGAC 1 cut(s) 197
FatI CATG 2 cut(s) 795, 813
FauI CCCGC 3 cut(s) 265, 340, 815
FbaI TGATCA 1 cut(s) 942
Fnu4HI GCNGC 6 cut(s) 296, 470, 663, 666, 714, 790
FokI GGATG 3 cut(s) 406, 553, 866
Fsp4HI GCNGC 6 cut(s) 296, 470, 663, 666, 714, 790
FspBI CTAG 3 cut(s) 225, 258, 659
GlaI GCGC 4 cut(s) 452, 612, 699, 769
GluI GCNGC 6 cut(s) 296, 470, 663, 666, 714, 790
GsuI CTGGAG 1 cut(s) 201
HaeII RGCGCY 1 cut(s) 771
HaeIII GGCC 6 cut(s) 298, 306, 491, 759, 914, 924
HapII CCGG 3 cut(s) 181, 269, 515
HgaI GACGC 2 cut(s) 234, 694
HhaI GCGC 4 cut(s) 453, 613, 700, 770
Hin1I GRCGYC 2 cut(s) 245, 686
Hin1II CATG 2 cut(s) 799, 817
Hin6I GCGC 4 cut(s) 451, 611, 698, 768
HinP1I GCGC 4 cut(s) 451, 611, 698, 768
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 899
HinfI GANTC 2 cut(s) 185, 236
HpaII CCGG 3 cut(s) 181, 269, 515
HphI GGTGA 3 cut(s) 372, 841, 896
Hpy166II GTNNAC 1 cut(s) 598
Hpy188I TCNGA 2 cut(s) 745, 765
Hpy188III TCNNGA 4 cut(s) 81, 233, 389, 784
Hpy8I GTNNAC 1 cut(s) 598
Hpy99I CGWCG 1 cut(s) 688
HpyAV CCTTC 5 cut(s) 69, 297, 369, 770, 857
HpyCH4III ACNGT 2 cut(s) 208, 709
HpyCH4IV ACGT 1 cut(s) 845
HpyCH4V TGCA 9 cut(s) 70, 163, 174, 534, 561, 636, 716, 729, 779
HpyF10VI GCNNNNNNNGC 6 cut(s) 638, 765, 776, 798, 819, 877
HpyF3I CTNAG 3 cut(s) 194, 719, 818
HpySE526I ACGT 1 cut(s) 845
Hsp92I GRCGYC 2 cut(s) 245, 686
Hsp92II CATG 2 cut(s) 799, 817
HspAI GCGC 4 cut(s) 451, 611, 698, 768
KpnI GGTACC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 942
Kzo9I GATC 3 cut(s) 94, 553, 942
Lsp1109I GCAGC 3 cut(s) 481, 677, 700
LweI GCATC 4 cut(s) 570, 736, 766, 826
MaeI CTAG 3 cut(s) 225, 258, 659
MaeII ACGT 1 cut(s) 845
MaeIII GTNAC 3 cut(s) 679, 829, 841
MalI GATC 3 cut(s) 96, 555, 944
MboI GATC 3 cut(s) 94, 553, 942
MboII GAAGA 4 cut(s) 127, 130, 274, 402
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 803
MlsI TGGCCA 1 cut(s) 914
MluCI AATT 3 cut(s) 9, 76, 394
MluNI TGGCCA 1 cut(s) 914
MlyI GAGTC 2 cut(s) 179, 230
MmeI TCCRAC 1 cut(s) 607
Mox20I TGGCCA 1 cut(s) 914
MscI TGGCCA 1 cut(s) 914
MseI TTAA 2 cut(s) 6, 807
Msp20I TGGCCA 1 cut(s) 914
MspI CCGG 3 cut(s) 181, 269, 515
MspR9I CCNGG 3 cut(s) 181, 182, 515
MvnI CGCG 6 cut(s) 295, 451, 453, 611, 700, 792
MwoI GCNNNNNNNGC 6 cut(s) 638, 765, 776, 798, 819, 877
NciI CCSGG 3 cut(s) 181, 182, 515
NdeII GATC 3 cut(s) 94, 553, 942
NlaIII CATG 2 cut(s) 799, 817
NlaIV GGNNCC 2 cut(s) 96, 200
NmuCI GTSAC 3 cut(s) 679, 829, 841
NspI RCATGY 1 cut(s) 817
PaeI GCATGC 1 cut(s) 817
PkrI GCNGC 6 cut(s) 297, 471, 664, 667, 715, 791
PleI GAGTC 2 cut(s) 179, 230
PpsI GAGTC 2 cut(s) 179, 230
PshBI ATTAAT 1 cut(s) 807
PspEI GGTNACC 1 cut(s) 829
PspN4I GGNNCC 2 cut(s) 96, 200
PspPI GGNCC 1 cut(s) 305
PsuI RGATCY 1 cut(s) 94
RsaI GTAC 2 cut(s) 200, 740
RsaNI GTAC 2 cut(s) 199, 739
SaqAI TTAA 2 cut(s) 6, 807
SatI GCNGC 6 cut(s) 296, 470, 663, 666, 714, 790
Sau3AI GATC 3 cut(s) 94, 553, 942
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 2 cut(s) 179, 230
ScrFI CCNGG 3 cut(s) 181, 182, 515
SduI GDGCHC 1 cut(s) 803
SetI ASST 9 cut(s) 200, 204, 226, 385, 462, 511, 597, 848, 903
SfaNI GCATC 4 cut(s) 570, 736, 766, 826
SgrAI CRCCGGYG 1 cut(s) 268
SmaI CCCGGG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SphI GCATGC 1 cut(s) 817
Sse9I AATT 3 cut(s) 9, 76, 394
SspMI CTAG 3 cut(s) 225, 258, 659
StyD4I CCNGG 3 cut(s) 179, 180, 513
StyI CCWWGG 2 cut(s) 591, 750
TaaI ACNGT 2 cut(s) 208, 709
TaiI ACGT 1 cut(s) 848
TaqI TCGA 1 cut(s) 188
TaqII GACCGA 1 cut(s) 848
TasI AATT 3 cut(s) 9, 76, 394
TatI WGTACW 1 cut(s) 738
TauI GCSGC 3 cut(s) 298, 665, 792
Tru1I TTAA 2 cut(s) 6, 807
Tru9I TTAA 2 cut(s) 6, 807
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 3 cut(s) 679, 829, 841
TseI GCWGC 3 cut(s) 469, 665, 713
Tsp45I GTSAC 3 cut(s) 679, 829, 841
TspDTI ATGAA 3 cut(s) 27, 279, 893
TspGWI ACGGA 1 cut(s) 148
TspMI CCCGGG 1 cut(s) 180
TspRI CASTG 1 cut(s) 700
VspI ATTAAT 1 cut(s) 807
XapI RAATTY 1 cut(s) 76
XceI RCATGY 1 cut(s) 817
XmaI CCCGGG 1 cut(s) 180
XspI CTAG 3 cut(s) 225, 258, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.