Rh4DG258300

pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0015910
Physical Location & Seq
Reverse (-)
47484610 .. 47485506
897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG258300.1

Sequence Viewer

Length: 897 bp
ATGGATCCCCTCAATCACTATTGTCAAATACCTTTCAACATTCTTTTGTTCTTCTTCGTCTTTTGCTTGAGTTCTGCAGAATCCGCATGCCCGCCAAAGAATCCATACTTGACTTATCTATTTCCCTCTCCAGCTCCAGACTGTCAACCATCTGTTCGCCCATCTAAGCCAACTCCTCCGTCAGCAATCCCAGCCTTATCTCTACCATCTTCAAATCTTAGACCACGGCTCCGGCAAATATTTCCTTCATCTCTTCAACCACCTGTGGCCTCCCCTCGGCCTCAAAACTCCGCTCCTACAGCCAGTTTTCCCCCACCATCATACTTGTCATCGTTTTTCCCTCGTCCTTTTTCTGGTTCCACCAAAACAAGCCCACGGAGCAACCTCTTTATGCAATCACGCCAGCTGAGTGCAGCCTCATCGCCATCACCAGCAGCTGTGGATCCAGCCGCTAAACAGCTATGTAACAACACCAGCTACACGGATCTTTGCATTTCATCTATTCTCCCTTTCCTTTCAGCACGAGGAAAGACATCTATAGATGCCGTTACGATGCTCCAGATGGTGATAAAGGCTTGCTCCATACACGCCCAGATGGCCCTGGCAGCTGCATCAAAATATTCCACGGAGCCGAACAATGCCCACCGAACGACCATGGCAATCATGGAGTGCAAGGATATGTATAGTGAGGCGTTGGATGGCCTTCAAACTGCATTGGATGCGATTCAGGACAAAGACATTGCCACTATTAACACTATGCTTAGCGGGGTAATTAGCGATGCACAGACATGTGAGGATGGTTTTGAAGGACATAAATCTCCACTTAGTGATTTTGATGATAAACTTCGTAAGATGGGCAGCAATTGCCTTGCCATTGCTTCTTTGATAAAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

298

Amino Acids

32.22

Weight (kDa)

8.3

Isoelectric Point (pI)

68.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 151 - 292 3.8e-26 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G36659
fragaria_vesca FvH4_4g19570
malus_domestica MD16G1155100.v1.1
prunus_persica Prupe.1G118800_v2.0.a1
pyrus_communis pycom13g13250
rosa_chinensis RchiOBHm_Chr4g0424831
rosa_laevigata RLG00000007417
rosa_multiflora Rmu_sc0003608.1_g000023
rosa_roxburghii Rroxscaffold_5G00367050
rosa_rugosa Rorug04G0199400
rosa_samantha Rh4AG257200 Rh4BG262500 Rh4CG273500 Rh4DG258300
rosa_wichuraiana Rw4G022190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 293
AciI CCGC 5 cut(s) 84, 92, 291, 450, 765
AclWI GGATC 4 cut(s) 12, 437, 450, 492
AdeI CACNNNGTG 1 cut(s) 827
AfiI CCNNNNNNNGG 2 cut(s) 276, 353
AflIII ACRYGT 1 cut(s) 788
AgsI TTSAA 5 cut(s) 37, 213, 257, 707, 806
AjnI CCWGG 1 cut(s) 600
AluBI AGCT 6 cut(s) 134, 406, 437, 460, 477, 608
AluI AGCT 6 cut(s) 134, 406, 437, 460, 477, 608
AlwI GGATC 4 cut(s) 12, 437, 450, 492
AlwNI CAGNNNCTG 1 cut(s) 437
AoxI GGCC 4 cut(s) 267, 278, 597, 700
ApeKI GCWGC 5 cut(s) 413, 434, 605, 608, 858
AspS9I GGNCC 1 cut(s) 598
AsuHPI GGTGA 2 cut(s) 420, 577
BamHI GGATCC 2 cut(s) 4, 442
BauI CACGAG 1 cut(s) 522
BbvI GCAGC 5 cut(s) 425, 446, 595, 617, 870
BceAI ACGGC 2 cut(s) 242, 530
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BciT130I CCWGG 1 cut(s) 602
BfmI CTRYAG 3 cut(s) 75, 297, 537
BglI GCCNNNNNGGC 1 cut(s) 596
BisI GCNGC 6 cut(s) 414, 435, 450, 606, 609, 859
BlpI GCTNAGC 1 cut(s) 761
BlsI GCNGC 6 cut(s) 415, 436, 451, 607, 610, 860
Bme1390I CCNGG 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 598
BmiI GGNNCC 5 cut(s) 6, 230, 358, 444, 630
BmrFI CCNGG 1 cut(s) 602
BmsI GCATC 5 cut(s) 532, 543, 620, 709, 769
BpmI CTGGAG 3 cut(s) 114, 120, 542
Bpu1102I GCTNAGC 1 cut(s) 761
BpuEI CTTGAG 1 cut(s) 88
BsaJI CCNNGG 6 cut(s) 224, 275, 374, 600, 624, 654
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 353
Bse1I ACTGG 1 cut(s) 303
Bse3DI GCAATG 2 cut(s) 738, 873
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 6 cut(s) 224, 275, 374, 600, 624, 654
BseGI GGATG 3 cut(s) 703, 724, 802
BseLI CCNNNNNNNGG 2 cut(s) 276, 353
BseMI GCAATG 2 cut(s) 738, 873
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 165
BseXI GCAGC 5 cut(s) 425, 446, 595, 617, 870
BseYI CCCAGC 1 cut(s) 190
BsgI GTGCAG 1 cut(s) 432
BshFI GGCC 4 cut(s) 269, 280, 599, 702
BsiSI CCGG 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 276, 353
BsnI GGCC 4 cut(s) 269, 280, 599, 702
Bsp143I GATC 3 cut(s) 4, 442, 484
Bsp1720I GCTNAGC 1 cut(s) 761
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 5 cut(s) 84, 92, 291, 450, 765
BspANI GGCC 4 cut(s) 269, 280, 599, 702
BspCNI CTCAG 1 cut(s) 399
BspLI GGNNCC 5 cut(s) 6, 230, 358, 444, 630
BspMAI CTGCAG 1 cut(s) 79
BspPI GGATC 4 cut(s) 12, 437, 450, 492
BsrBI CCGCTC 1 cut(s) 293
BsrDI GCAATG 2 cut(s) 738, 873
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 6 cut(s) 224, 275, 374, 600, 624, 654
BssMI GATC 3 cut(s) 4, 442, 484
BssSI CACGAG 1 cut(s) 522
BssT1I CCWWGG 1 cut(s) 654
Bst2BI CACGAG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 258
BstAPI GCANNNNNTGC 2 cut(s) 719, 864
BstC8I GCNNGC 4 cut(s) 88, 92, 404, 577
BstDEI CTNAG 5 cut(s) 165, 218, 407, 761, 824
BstDSI CCRYGG 4 cut(s) 224, 374, 624, 654
BstF5I GGATG 3 cut(s) 703, 724, 802
BstKTI GATC 3 cut(s) 7, 445, 487
BstMBI GATC 3 cut(s) 4, 442, 484
BstMWI GCNNNNNNNGC 8 cut(s) 83, 191, 299, 378, 596, 605, 719, 864
BstNI CCWGG 1 cut(s) 602
BstNSI RCATGY 2 cut(s) 90, 792
BstSCI CCNGG 1 cut(s) 600
BstSFI CTRYAG 3 cut(s) 75, 297, 537
BstV1I GCAGC 5 cut(s) 425, 446, 595, 617, 870
BstX2I RGATCY 3 cut(s) 4, 442, 484
BstYI RGATCY 3 cut(s) 4, 442, 484
BsuRI GGCC 4 cut(s) 269, 280, 599, 702
BtgI CCRYGG 4 cut(s) 224, 374, 624, 654
BtgZI GCGATG 2 cut(s) 405, 792
BtsCI GGATG 3 cut(s) 703, 724, 802
Cac8I GCNNGC 4 cut(s) 88, 92, 404, 577
CaiI CAGNNNCTG 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 598
CviAII CATG 4 cut(s) 87, 655, 664, 789
DdeI CTNAG 5 cut(s) 165, 218, 407, 761, 824
DpnI GATC 3 cut(s) 6, 444, 486
DpnII GATC 3 cut(s) 4, 442, 484
DraIII CACNNNGTG 1 cut(s) 827
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 654
EcoRII CCWGG 1 cut(s) 600
EcoT14I CCWWGG 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 654
FaeI CATG 4 cut(s) 90, 658, 667, 792
FatI CATG 4 cut(s) 86, 654, 663, 788
FauI CCCGC 2 cut(s) 99, 758
Fnu4HI GCNGC 6 cut(s) 414, 435, 450, 606, 609, 859
FokI GGATG 3 cut(s) 710, 731, 809
Fsp4HI GCNGC 6 cut(s) 414, 435, 450, 606, 609, 859
GluI GCNGC 6 cut(s) 414, 435, 450, 606, 609, 859
GsaI CCCAGC 1 cut(s) 194
GsuI CTGGAG 3 cut(s) 114, 120, 542
HaeIII GGCC 4 cut(s) 269, 280, 599, 702
HapII CCGG 1 cut(s) 232
Hin1II CATG 4 cut(s) 90, 658, 667, 792
HincII GTYRAC 1 cut(s) 146
HindII GTYRAC 1 cut(s) 146
HinfI GANTC 3 cut(s) 80, 100, 724
HpaII CCGG 1 cut(s) 232
HphI GGTGA 2 cut(s) 420, 577
Hpy166II GTNNAC 1 cut(s) 146
Hpy188III TCNNGA 3 cut(s) 137, 559, 728
Hpy8I GTNNAC 1 cut(s) 146
HpyAV CCTTC 3 cut(s) 255, 713, 800
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4V TGCA 8 cut(s) 77, 394, 413, 492, 611, 672, 713, 782
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 191, 299, 378, 596, 605, 719, 864
HpyF3I CTNAG 5 cut(s) 165, 218, 407, 761, 824
Hsp92II CATG 4 cut(s) 90, 658, 667, 792
Kzo9I GATC 3 cut(s) 4, 442, 484
LmnI GCTCC 7 cut(s) 139, 234, 298, 378, 561, 584, 628
Lsp1109I GCAGC 5 cut(s) 425, 446, 595, 617, 870
LweI GCATC 5 cut(s) 532, 543, 620, 709, 769
MaeIII GTNAC 2 cut(s) 464, 547
MalI GATC 3 cut(s) 6, 444, 486
MbiI CCGCTC 1 cut(s) 293
MboI GATC 3 cut(s) 4, 442, 484
MboII GAAGA 4 cut(s) 43, 46, 201, 245
MfeI CAATTG 1 cut(s) 862
MflI RGATCY 3 cut(s) 4, 442, 484
MluCI AATT 2 cut(s) 771, 862
MmeI TCCRAC 1 cut(s) 675
MseI TTAA 1 cut(s) 750
MslI CAYNNNNRTG 1 cut(s) 787
MspA1I CMGCKG 3 cut(s) 406, 437, 608
MspI CCGG 1 cut(s) 232
MspR9I CCNGG 1 cut(s) 602
MunI CAATTG 1 cut(s) 862
MvaI CCWGG 1 cut(s) 602
MwoI GCNNNNNNNGC 8 cut(s) 83, 191, 299, 378, 596, 605, 719, 864
NcoI CCATGG 1 cut(s) 654
NdeII GATC 3 cut(s) 4, 442, 484
NlaIII CATG 4 cut(s) 90, 658, 667, 792
NlaIV GGNNCC 5 cut(s) 6, 230, 358, 444, 630
NmeAIII GCCGAG 1 cut(s) 256
NspI RCATGY 2 cut(s) 90, 792
PaeI GCATGC 1 cut(s) 90
PciI ACATGT 1 cut(s) 788
PfeI GAWTC 3 cut(s) 80, 100, 724
PkrI GCNGC 6 cut(s) 415, 436, 451, 607, 610, 860
PscI ACATGT 1 cut(s) 788
Psp6I CCWGG 1 cut(s) 600
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 5 cut(s) 6, 230, 358, 444, 630
PspPI GGNCC 1 cut(s) 598
PstI CTGCAG 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 437
PsuI RGATCY 3 cut(s) 4, 442, 484
PvuII CAGCTG 3 cut(s) 406, 437, 608
RseI CAYNNNNRTG 1 cut(s) 787
SaqAI TTAA 1 cut(s) 750
SatI GCNGC 6 cut(s) 414, 435, 450, 606, 609, 859
Sau3AI GATC 3 cut(s) 4, 442, 484
Sau96I GGNCC 1 cut(s) 598
ScrFI CCNGG 1 cut(s) 602
SetI ASST 9 cut(s) 34, 136, 265, 387, 408, 439, 462, 479, 610
SfaNI GCATC 5 cut(s) 532, 543, 620, 709, 769
SfcI CTRYAG 3 cut(s) 75, 297, 537
SmiMI CAYNNNNRTG 1 cut(s) 787
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
SphI GCATGC 1 cut(s) 90
Sse9I AATT 2 cut(s) 771, 862
SsiI CCGC 5 cut(s) 84, 92, 291, 450, 765
SspI AATATT 2 cut(s) 240, 620
StyD4I CCNGG 1 cut(s) 600
StyI CCWWGG 1 cut(s) 654
TaaI ACNGT 1 cut(s) 143
TasI AATT 2 cut(s) 771, 862
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 3 cut(s) 80, 100, 724
Tru1I TTAA 1 cut(s) 750
Tru9I TTAA 1 cut(s) 750
TseI GCWGC 5 cut(s) 413, 434, 605, 608, 858
TspDTI ATGAA 2 cut(s) 237, 486
TspGWI ACGGA 4 cut(s) 168, 391, 497, 641
XceI RCATGY 2 cut(s) 90, 792
XcmI CCANNNNNNNNNTGG 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.