Rroxscaffold_5G00367050

pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015910
Physical Location & Seq
Reverse (-)
48991642 .. 48992535
894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367050.1

Sequence Viewer

Length: 894 bp
ATGGATCCCCTCAATAACTATTGTCAAATACCTTTCAACATTCTTTTGTTCTTCTTCGTCTTTTGCTTGAGTTCTGCAGAAGCCGCATGCATGCCAAAGAATCCATACTTGACTTATCTATTTCCCTCTCCAGGTCCAGACTGCCAACCATCTGTTGCCCCATCTGAGCCAACTCCTCCGTCAGCAATCCCAGCCTTATCTCTACCATCTTCAAATCTTAAACCACGGCTCCAGCAAATATTTCCTTCATCTCTTCAACCACCTGTGGCCTCCCCTCGGCCTCAAAACTCCGTTCCTACAGCCAGTTTTTCCTCACCATCATACTTGTCATCGTTTTCTCGTCCTTTTTCTGGTTCCACCAAAACAAGCCCACGGAGCAACCTCTTTATGCAATCACGCCAGCTGAGTGCAGCCTCATCGCCATCACCAGCAGCTGTGGATCCAGCCGCTAAACAGCTATGTAACAACACCAACTACACGGATCTTTGCATTTCATCTATTCTCCCTTTCCTTTCAGCACGAGGAAAGACATCTATAGATGCCGTTACGATGCTCCAGATGGTGATAAAGGCTTGCTCCATACACGCCCAGATGGCCCTGGCAGCTGCATCAAAACACTCCACGGAGCCGAACAATGCCCACCGAACGACCATGGCAATCATGGAGTGCAAGGATATGTATAGTGAGGCGTTGGATGGCCTTCAAACTGCATTGGATGCGATTCTGGACAAAGACATTGCCACTATTAACACTATGCTTAGCGGGGTAATTAGTGATGCACAGACATGTGAGGATGGTTTTGAAGGACATAAATCTCCACTTAGTGATTTTGATGATAAACTTCGTAAGATGGGCAGCAATTGCCTTGCCATTGCTTCTTTGATAAAGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

297

Amino Acids

31.9

Weight (kDa)

6.41

Isoelectric Point (pI)

66.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 150 - 291 2.1e-25 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G36659
fragaria_vesca FvH4_4g19570
malus_domestica MD16G1155100.v1.1
prunus_persica Prupe.1G118800_v2.0.a1
pyrus_communis pycom13g13250
rosa_chinensis RchiOBHm_Chr4g0424831
rosa_laevigata RLG00000007417
rosa_multiflora Rmu_sc0003608.1_g000023
rosa_roxburghii Rroxscaffold_5G00367050
rosa_rugosa Rorug04G0199400
rosa_samantha Rh4AG257200 Rh4BG262500 Rh4CG273500 Rh4DG258300
rosa_wichuraiana Rw4G022190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 84, 447, 762
AclWI GGATC 4 cut(s) 12, 434, 447, 489
AdeI CACNNNGTG 1 cut(s) 824
AfiI CCNNNNNNNGG 3 cut(s) 131, 276, 350
AflIII ACRYGT 1 cut(s) 785
AgsI TTSAA 5 cut(s) 37, 213, 257, 704, 803
AjnI CCWGG 2 cut(s) 130, 597
AluBI AGCT 4 cut(s) 403, 434, 457, 605
AluI AGCT 4 cut(s) 403, 434, 457, 605
AlwI GGATC 4 cut(s) 12, 434, 447, 489
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 4 cut(s) 267, 278, 594, 697
ApeKI GCWGC 5 cut(s) 410, 431, 602, 605, 855
AspS9I GGNCC 2 cut(s) 134, 595
AsuHPI GGTGA 3 cut(s) 306, 417, 574
AvaII GGWCC 1 cut(s) 134
BamHI GGATCC 2 cut(s) 4, 439
BauI CACGAG 1 cut(s) 519
BbvI GCAGC 5 cut(s) 422, 443, 592, 614, 867
BceAI ACGGC 2 cut(s) 242, 527
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BciT130I CCWGG 2 cut(s) 132, 599
BfmI CTRYAG 3 cut(s) 75, 297, 534
BglI GCCNNNNNGGC 1 cut(s) 593
BisI GCNGC 7 cut(s) 84, 411, 432, 447, 603, 606, 856
BlpI GCTNAGC 1 cut(s) 758
BlsI GCNGC 7 cut(s) 85, 412, 433, 448, 604, 607, 857
Bme1390I CCNGG 2 cut(s) 132, 599
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 2 cut(s) 134, 595
BmiI GGNNCC 5 cut(s) 6, 230, 355, 441, 627
BmrFI CCNGG 2 cut(s) 132, 599
BmsI GCATC 5 cut(s) 529, 540, 617, 706, 766
BpmI CTGGAG 3 cut(s) 114, 215, 539
Bpu1102I GCTNAGC 1 cut(s) 758
BpuEI CTTGAG 1 cut(s) 88
BsaJI CCNNGG 6 cut(s) 224, 275, 371, 597, 621, 651
BsaXI ACNNNNNCTCC 2 cut(s) 213, 243
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 276, 350
Bse1I ACTGG 1 cut(s) 303
Bse3DI GCAATG 2 cut(s) 735, 870
BseBI CCWGG 2 cut(s) 132, 599
BseDI CCNNGG 6 cut(s) 224, 275, 371, 597, 621, 651
BseGI GGATG 3 cut(s) 700, 721, 799
BseLI CCNNNNNNNGG 3 cut(s) 131, 276, 350
BseMI GCAATG 2 cut(s) 735, 870
BseMII CTCAG 2 cut(s) 156, 395
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 165
BseXI GCAGC 5 cut(s) 422, 443, 592, 614, 867
BseYI CCCAGC 1 cut(s) 190
BsgI GTGCAG 1 cut(s) 429
BshFI GGCC 4 cut(s) 269, 280, 596, 699
BslI CCNNNNNNNGG 3 cut(s) 131, 276, 350
BsnI GGCC 4 cut(s) 269, 280, 596, 699
Bsp143I GATC 3 cut(s) 4, 439, 481
Bsp1720I GCTNAGC 1 cut(s) 758
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 3 cut(s) 84, 447, 762
BspANI GGCC 4 cut(s) 269, 280, 596, 699
BspCNI CTCAG 2 cut(s) 157, 396
BspLI GGNNCC 5 cut(s) 6, 230, 355, 441, 627
BspMAI CTGCAG 1 cut(s) 79
BspPI GGATC 4 cut(s) 12, 434, 447, 489
BsrDI GCAATG 2 cut(s) 735, 870
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 6 cut(s) 224, 275, 371, 597, 621, 651
BssMI GATC 3 cut(s) 4, 439, 481
BssSI CACGAG 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 651
Bst2BI CACGAG 1 cut(s) 519
Bst2UI CCWGG 2 cut(s) 132, 599
Bst6I CTCTTC 1 cut(s) 258
BstAPI GCANNNNNTGC 2 cut(s) 716, 861
BstC8I GCNNGC 4 cut(s) 88, 92, 401, 574
BstDEI CTNAG 4 cut(s) 165, 404, 758, 821
BstDSI CCRYGG 4 cut(s) 224, 371, 621, 651
BstF5I GGATG 3 cut(s) 700, 721, 799
BstKTI GATC 3 cut(s) 7, 442, 484
BstMBI GATC 3 cut(s) 4, 439, 481
BstMWI GCNNNNNNNGC 7 cut(s) 83, 191, 375, 593, 602, 716, 861
BstNI CCWGG 2 cut(s) 132, 599
BstNSI RCATGY 3 cut(s) 90, 94, 789
BstSCI CCNGG 2 cut(s) 130, 597
BstSFI CTRYAG 3 cut(s) 75, 297, 534
BstV1I GCAGC 5 cut(s) 422, 443, 592, 614, 867
BstX2I RGATCY 3 cut(s) 4, 439, 481
BstYI RGATCY 3 cut(s) 4, 439, 481
BsuRI GGCC 4 cut(s) 269, 280, 596, 699
BtgI CCRYGG 4 cut(s) 224, 371, 621, 651
BtgZI GCGATG 1 cut(s) 402
BtsCI GGATG 3 cut(s) 700, 721, 799
Cac8I GCNNGC 4 cut(s) 88, 92, 401, 574
CaiI CAGNNNCTG 1 cut(s) 434
Cfr13I GGNCC 2 cut(s) 134, 595
CviAII CATG 5 cut(s) 87, 91, 652, 661, 786
DdeI CTNAG 4 cut(s) 165, 404, 758, 821
DpnI GATC 3 cut(s) 6, 441, 483
DpnII GATC 3 cut(s) 4, 439, 481
DraIII CACNNNGTG 1 cut(s) 824
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 651
Eco47I GGWCC 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 130, 597
EcoT14I CCWWGG 1 cut(s) 651
EcoT22I ATGCAT 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 651
FaeI CATG 5 cut(s) 90, 94, 655, 664, 789
FatI CATG 5 cut(s) 86, 90, 651, 660, 785
FauI CCCGC 1 cut(s) 755
Fnu4HI GCNGC 7 cut(s) 84, 411, 432, 447, 603, 606, 856
FokI GGATG 3 cut(s) 707, 728, 806
Fsp4HI GCNGC 7 cut(s) 84, 411, 432, 447, 603, 606, 856
GluI GCNGC 7 cut(s) 84, 411, 432, 447, 603, 606, 856
GsaI CCCAGC 1 cut(s) 194
GsuI CTGGAG 3 cut(s) 114, 215, 539
HaeIII GGCC 4 cut(s) 269, 280, 596, 699
Hin1II CATG 5 cut(s) 90, 94, 655, 664, 789
HinfI GANTC 2 cut(s) 100, 721
HphI GGTGA 3 cut(s) 306, 417, 574
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 3 cut(s) 137, 556, 725
HpyAV CCTTC 3 cut(s) 255, 710, 797
HpyCH4V TGCA 9 cut(s) 77, 90, 391, 410, 489, 608, 669, 710, 779
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 191, 375, 593, 602, 716, 861
HpyF3I CTNAG 4 cut(s) 165, 404, 758, 821
Hsp92II CATG 5 cut(s) 90, 94, 655, 664, 789
Kzo9I GATC 3 cut(s) 4, 439, 481
LmnI GCTCC 5 cut(s) 234, 375, 558, 581, 625
Lsp1109I GCAGC 5 cut(s) 422, 443, 592, 614, 867
LweI GCATC 5 cut(s) 529, 540, 617, 706, 766
MaeIII GTNAC 2 cut(s) 461, 544
MalI GATC 3 cut(s) 6, 441, 483
MboI GATC 3 cut(s) 4, 439, 481
MboII GAAGA 4 cut(s) 43, 46, 201, 245
MfeI CAATTG 1 cut(s) 859
MflI RGATCY 3 cut(s) 4, 439, 481
MluCI AATT 2 cut(s) 768, 859
MmeI TCCRAC 1 cut(s) 672
Mph1103I ATGCAT 1 cut(s) 92
MseI TTAA 2 cut(s) 219, 747
MslI CAYNNNNRTG 1 cut(s) 784
MspA1I CMGCKG 3 cut(s) 403, 434, 605
MspR9I CCNGG 2 cut(s) 132, 599
MunI CAATTG 1 cut(s) 859
MvaI CCWGG 2 cut(s) 132, 599
MwoI GCNNNNNNNGC 7 cut(s) 83, 191, 375, 593, 602, 716, 861
NcoI CCATGG 1 cut(s) 651
NdeII GATC 3 cut(s) 4, 439, 481
NlaIII CATG 5 cut(s) 90, 94, 655, 664, 789
NlaIV GGNNCC 5 cut(s) 6, 230, 355, 441, 627
NmeAIII GCCGAG 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 92
NspI RCATGY 3 cut(s) 90, 94, 789
PaeI GCATGC 2 cut(s) 90, 94
PciI ACATGT 1 cut(s) 785
PfeI GAWTC 2 cut(s) 100, 721
PkrI GCNGC 7 cut(s) 85, 412, 433, 448, 604, 607, 857
PscI ACATGT 1 cut(s) 785
Psp6I CCWGG 2 cut(s) 130, 597
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 2 cut(s) 130, 597
PspN4I GGNNCC 5 cut(s) 6, 230, 355, 441, 627
PspPI GGNCC 2 cut(s) 134, 595
PstI CTGCAG 1 cut(s) 79
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 3 cut(s) 4, 439, 481
PvuII CAGCTG 3 cut(s) 403, 434, 605
RseI CAYNNNNRTG 1 cut(s) 784
SaqAI TTAA 2 cut(s) 219, 747
SatI GCNGC 7 cut(s) 84, 411, 432, 447, 603, 606, 856
Sau3AI GATC 3 cut(s) 4, 439, 481
Sau96I GGNCC 2 cut(s) 134, 595
ScrFI CCNGG 2 cut(s) 132, 599
SetI ASST 8 cut(s) 34, 136, 265, 384, 405, 436, 459, 607
SfaNI GCATC 5 cut(s) 529, 540, 617, 706, 766
SfcI CTRYAG 3 cut(s) 75, 297, 534
SinI GGWCC 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 784
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
SphI GCATGC 2 cut(s) 90, 94
Sse9I AATT 2 cut(s) 768, 859
SsiI CCGC 3 cut(s) 84, 447, 762
SspI AATATT 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 130, 597
StyI CCWWGG 1 cut(s) 651
TasI AATT 2 cut(s) 768, 859
TauI GCSGC 2 cut(s) 86, 449
TfiI GAWTC 2 cut(s) 100, 721
Tru1I TTAA 2 cut(s) 219, 747
Tru9I TTAA 2 cut(s) 219, 747
TseI GCWGC 5 cut(s) 410, 431, 602, 605, 855
TspDTI ATGAA 2 cut(s) 237, 483
TspGWI ACGGA 5 cut(s) 168, 280, 388, 494, 638
VpaK11BI GGWCC 1 cut(s) 134
XceI RCATGY 3 cut(s) 90, 94, 789
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
Zsp2I ATGCAT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.